A composition, preparation method and application for improving male reproductive ability

Abstract

The present invention provides a composition, a preparation method and an application for improving male reproductive ability, belonging to the field of bioengineering technology. It is prepared from the following raw materials by weight: 10-30 parts of Bacillus subtilis fibrinolytic enzyme, 5-15 parts of Haematococcus pluvialis, 3-10 parts of walnut kernel extract, 3-10 parts of Cordyceps militaris extract, and 0.3-0.5 parts of rice bran alkanol; the Bacillus subtilis fibrinolytic enzyme is prepared by fermenting the strain Bacillus subtilis QK02, and the strain is preserved in the China Center for Type Culture Collection with the preservation number CCTCC NO: M 203078. The present invention provides a Bacillus subtilis fibrinolytic enzyme composition with the efficacy of improving male reproductive ability, rationally compatible with medicinal and edible ingredients, having a significant synergistic effect, promoting human blood circulation and metabolism, eliminating fatigue, supplementing androgen, and significantly improving human immunity.

Description

Technical Field

[0001] The present invention relates to the field of bioengineering technology, and particularly relates to a composition for improving male reproductive ability, a preparation method and an application thereof. Background Art

[0002] With the improvement of people's living standards, their awareness of their own health is also gradually increasing. However, due to the influence of various factors such as the change of diet structure, the acceleration of life rhythm, psychological and environmental factors, and obesity of modern people, the phenomenon of declining male reproductive ability has emerged. The blood testosterone level of today's young men is generally low, and the incidence of sexual dysfunction is increasing. The testosterone level and sexual function of young people are often inferior to those of elderly men aged 50 - 70 years old. This seriously affects the quality of life and happiness index of young men.

[0003] The phenomenon of the general decline in male reproductive ability has attracted the attention of researchers. Epidemiological studies show that continuous excessive electromagnetic radiation is likely to damage the testicles and cause male sexual function and reproductive function disorders. Electromagnetic radiation can also reduce the male androgen level, disrupt male sexual function, and bring diseases of important androgen-related organs. Environmental estrogen is mainly bisphenol A. Its toxic effect mainly damages the male reproductive system and directly affects male fertility. Nicotine can cause DNA breaks in spermatogenic cells (spermatogonia, spermatocytes) in the male reproductive system, promote apoptosis of Leydig cells and Sertoli cells; cause abnormal sperm morphology, reduce sperm motility and hinder the fertilization process; promote sperm apoptosis and reduce sperm activity, etc. High blood sugar can increase the oxidative stress response of sperm, thereby damaging the integrity of sperm DNA and ultimately leading to a decline in fertility. Hyperlipidemia has an adverse effect on sperm function. High cholesterol and high phospholipids often lead to incomplete acrosome and head structure of sperm. Obesity can also induce many problems that affect reproductive ability, such as erectile dysfunction (ED), scrotal lipomatosis, low levels of serum total testosterone (TT) and free testosterone (FT) in androgens, and increased estrogen, etc.

[0004] Drugs for treating male reproductive ability mainly treat by supplementing androgens and inhibitors. Although inhibitor drugs have good curative effects, some adverse reactions will also occur after taking them, mainly including headache, nausea, dry mouth, and diarrhea. The side effects are large, and it is extremely easy to relapse after stopping the drug.

[0005] Bacillus subtilis fibrinolytic enzyme is prepared from soybean peptone through liquid fermentation of Bacillus subtilis, followed by concentration and drying. A variety of physiologically active substances are produced during the fermentation process, including a large amount of Bacillus subtilis fibrinolytic enzyme, vitamins, etc. It has the functions of dissolving fibrin and thrombus in the body, dissolving old thrombus plaques, reducing blood lipid and blood sugar, promoting human blood circulation and metabolism, eliminating fatigue, and enhancing human immunity. However, there is currently no report on the application of a composition with Bacillus subtilis fibrinolytic enzyme as the main component, reasonable formulation, and high activity in the field of health products for improving male reproductive ability. Summary of the Invention

[0006] The object of the present invention is to provide a composition, preparation method, and application for improving male reproductive ability. By reasonably combining food and medicine ingredients, it has a significant synergistic effect, promotes human blood circulation and metabolism, eliminates fatigue, supplements androgen, and significantly enhances human immunity.

[0007] The technical solution of the present invention is realized as follows:

[0008] The present invention provides a composition for improving male reproductive ability, which is prepared from the following raw materials by weight: 10 - 30 parts of Bacillus subtilis fibrinolytic enzyme, 5 - 15 parts of Haematococcus pluvialis, 3 - 10 parts of walnut kernel extract, 3 - 10 parts of Cordyceps militaris extract, and 0.3 - 0.5 parts of rice bran aliphatic alcohol; the Bacillus subtilis fibrinolytic enzyme is prepared by fermenting the strain Bacillus subtilis QK02, and the strain is preserved in the China Center for Type Culture Collection with the preservation number CCTCC NO:M 203078.

[0009] As a further improvement of the present invention, the Bacillus subtilis fibrinolytic enzyme is prepared by fermenting the strain Bacillus subtilis QK02, followed by centrifugation, concentration, and drying.

[0010] As a further improvement of the present invention, the preparation method of the Bacillus subtilis fibrinolytic enzyme is as follows:

[0011] S1. Seed liquid preparation; The strain Bacillus subtilis QK02 is stored in a -80°C low-temperature refrigerator and activated on a fresh slant for 18 - 24 h before use. One loop of bacteria is picked from the slant and inoculated into a triangular flask containing LB liquid medium, and cultured on a shaker at 35 - 40°C and 100 - 500 r / min for 15 - 20 h;

[0012] S2. Fermentation culture: Sterilize the fermentation medium at 110 - 115°C for 10 - 30 min. After cooling to room temperature, inoculate the above-mentioned seed liquid into the fermentation medium at an inoculation amount of 1%, and start fermentation for 20 - 30 h. Among them, control the temperature at 35 - 40°C, humidity at 80 - 90%, and dissolved oxygen at 20 - 40% in the fermentation equipment.

[0013] S3. Post-treatment: Centrifuge the obtained fermentation broth with a tubular centrifuge, concentrate it by microfiltration and ultrafiltration to obtain a concentrated solution. The concentrated solution is spray-dried at an inlet air temperature of 120 - 150°C and an outlet air temperature of 63 - 68°C to obtain Bacillus subtilis fibrinolytic enzyme.

[0014] As a further improvement of the present invention, the walnut extract is an ethanol extract of walnuts.

[0015] Preferably, the walnut extract is prepared by the following method: Crush walnuts, add an 85% ethanol solution by a material-liquid ratio of 1:10, reflux and extract at 60°C for 2 - 3 times, with each extraction time of 2 - 4 h. Filter while it is hot, combine the filtrates, and concentrate under reduced pressure to obtain an extract without alcohol smell, which is the walnut extract.

[0016] As a further improvement of the present invention, the Cordyceps militaris extract contains cordycepin.

[0017] Preferably, the Cordyceps militaris extract is prepared by the following method: Crush Cordyceps militaris, add 8 - 20 times the weight of water, extract at 80 - 105°C for 1 - 3 times, extract for 2 - 6 h, filter the extract, combine them, concentrate to a solid content of 5% - 25% to obtain a concentrated Cordyceps militaris extract solution, and dry the concentrated Cordyceps militaris extract solution to obtain the Cordyceps militaris extract.

[0018] As a further improvement of the present invention, the Haematococcus pluvialis contains astaxanthin.

[0019] Preferably, the Haematococcus pluvialis is prepared by the following method: Using the algal bodies and spores of Haematococcus pluvialis as raw materials, stir and break the cell walls at 400 - 800 revolutions per minute with a high-speed dispersion mixer, and dry with hot air at 60 - 80°C until the water content is below 10% to obtain Haematococcus pluvialis powder.

[0020] As a further improvement of the present invention, the rice bran alkanol contains octacosanol.

[0021] As a further improvement of the present invention, the composition further includes acceptable excipients.

[0022] The present invention further protects a method for preparing the above-mentioned composition for improving male reproductive ability, which comprises mixing Bacillus subtilis fibrinolytic enzyme, Haematococcus pluvialis, walnut kernel extract, Cordyceps militaris extract, and rice bran fatty alkyl alcohol in proportion to obtain the composition for improving male reproductive ability.

[0023] Preferably, the composition for improving male reproductive ability is prepared into one of capsules, tablets, powders and pills.

[0024] The present invention further protects the use of the above-mentioned composition for improving male reproductive ability in the preparation of food, health food and medicine for improving male reproductive ability.

[0025] Bacillus subtilis fibrinolytic enzyme is made from soy peptone, fermented by Bacillus subtilis liquid, concentrated and dried. It contains Bacillus subtilis fibrinolytic enzyme, vitamin K2 soy peptide and other functional factors.

[0026] Haematococcus pluvialis is a freshwater unicellular green algae. The algae can accumulate a large amount of astaxanthin and appear red, hence the name Haematococcus pluvialis. Its astaxanthin content can reach 3.0% of dry weight, or even higher, and is the best biological source of natural astaxanthin. The natural astaxanthin of Haematococcus pluvialis can enhance aerobic metabolism, reduce muscle pain, improve endurance; reduce muscle damage and shorten recovery time; promote fat burning and obtain higher energy levels; maintain joint function after intense exercise and prevent obesity.

[0027] Walnut kernels are rich in amino acids, crude protein and crude lipids, which help maintain human vitality. In addition, the vitamin E contained in walnut kernels can protect cells from oxidative damage by free radicals and is recognized by the medical community as an anti-aging substance.

[0028] Cordyceps militaris is mild in nature and sweet in taste. It benefits the lungs and kidneys, stops bleeding and reduces phlegm. It can cure all kinds of deficiencies and injuries. It is rich in cordycepic acid, cordycepin, ergosterol, etc., which can calm the nerves and resist various bacteria.

[0029] Rice bran fatty alcohol is prepared from rice bran. Rice bran wax is saponified, extracted, filtered and other processes to make it. The main components include fatty alcohols such as octacosanol, triacontanol, dotriacontanol, etc. It has functions such as enhancing physical strength, endurance, energy, improving reaction sensitivity and stress ability, and increasing the body's metabolic rate. Therefore, according to the formula of the present invention, each component is used in combination, with strong biological activity, comprehensive efficacy, and safe to eat. It realizes the organic integration of nutritional components, efficacy, and action mechanism, and obtains the effect of mutual promotion and synergistic enhancement among various components. The rationally formulated Bacillus subtilis fibrinolytic enzyme composition better adapts to the needs of the human body, has a significant effect on improving male reproductive ability, and when each component of the Bacillus subtilis fibrinolytic enzyme composition is used in combination, it can be lower than the dosage of each component alone or the combination of main efficacy components, achieving the effect that the comprehensive efficacy surpasses and improves the use of each component alone.

[0030] The present invention has the following beneficial effects:

[0031] The present invention provides a Bacillus subtilis fibrinolytic enzyme composition with the effect of improving male reproductive ability and its preparation method. The edible and medicinal ingredients are rationally formulated, with a significant synergistic effect, promoting human blood circulation and metabolism, eliminating fatigue, supplementing androgen, and significantly improving human immunity. Detailed implementation mode

[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0033] Bacillus subtilis QK02 strain, the strain is preserved in the China Center for Type Culture Collection, the preservation location is Wuhan University, China, and its preservation number is CCTCC NO: M 203078, and the preservation date is November 17, 2003.

[0034] Example 1

[0035] A composition for improving male reproductive ability and its preparation method, including the following steps:

[0036] I. Preparation of Bacillus subtilis fibrinolytic enzyme

[0037] (I) Seed liquid preparation

[0038] The strain Bacillus subtilis QK02 was stored in a -80 °C low-temperature refrigerator. Before use, the strain was activated on a fresh slant for 24 h. A loopful of cells was picked from the slant and inoculated into a triangular flask containing LB liquid medium, and cultured on a shaker at 37 °C and 200 r / min for 18 h.

[0039] (II) Fermentation culture in a fermenter (500 L)

[0040] 300 L of the fermentation medium in Table 1 was prepared, sterilized at 115 °C for 30 min, and after cooling to room temperature, the above-mentioned seed liquid was inoculated into the fermentation medium at an inoculation amount of 1%, and fermentation was started for 24 h. Among them, the temperature was controlled at 37 °C, the humidity was 85%, and the dissolved oxygen was 30% in the fermentation equipment.

[0041] Table 1 Medium formula

[0042] Name Proportion Peptone 2％ Glucose 2％ Disodium hydrogen phosphate 1％ Sodium dihydrogen phosphate 0.1％ Magnesium sulfate 0.05％ Anhydrous calcium chloride 0.02％ Defoamer 0.1％ Deionized water Balance

[0043] (III) Preparation of Bacillus subtilis fibrinolytic enzyme

[0044] After fermentation, the fermentation broth was centrifuged by a tubular centrifuge, and concentrated by microfiltration and ultrafiltration to obtain about 25 L of concentrated liquid. The concentrated liquid was spray-dried at an inlet air temperature of 140 °C and an outlet air temperature of 65 °C to obtain Bacillus subtilis fibrinolytic enzyme.

[0045] II. Preparation of Haematococcus pluvialis: Using Haematococcus pluvialis algal bodies and spores as raw materials, they were stirred and broken by a high-speed dispersing mixer at 600 r / min and dried by blowing air at 75 °C until the water content reached 8% to obtain Haematococcus pluvialis powder.

[0046] III. Preparation of walnut kernel extract: The walnut kernels were crushed, added with an ethanol solution with a volume fraction of 85% at a solid-to-liquid ratio of 1:10, refluxed and extracted 3 times at 60 °C, each extraction time was 3 h, filtered while hot, the filtrates were combined, and concentrated under reduced pressure to obtain an extract without alcohol smell, which was the walnut kernel extract.

[0047] IV. Preparation of Cordyceps militaris extract: The Cordyceps militaris was crushed, added with 12 times the weight of water, extracted 2 times at 90 °C for 4 h, the extraction liquid was filtered, combined, and concentrated to a solid content of 18% to obtain the Cordyceps militaris extraction concentrate, and the Cordyceps militaris extraction concentrate was dried to obtain the Cordyceps militaris extract.

[0048] V. After all raw materials passed through an 80-mesh sieve, they were mixed according to the ratio of 20 parts of Bacillus subtilis fibrinolytic enzyme, 10 parts of Haematococcus pluvialis, 5 parts of walnut kernel extract, 5 parts of Cordyceps militaris extract, and 0.4 part of rice bran alkanol to prepare the composition of the present invention.

[0049] Example 2

[0050] Compared with Example 1, the raw material ratio is different.

[0051] Raw material composition (parts by weight): 10 parts of Bacillus subtilis fibrinolytic enzyme, 5 parts of Haematococcus pluvialis, 3 parts of walnut kernel extract, 3 parts of Cordyceps militaris extract, 0.3 part of rice bran alkanol.

[0052] Example 3

[0053] Compared with Example 1, the raw material ratio is different.

[0054] Raw material composition (parts by weight): 30 parts of Bacillus subtilis fibrinolytic enzyme, 15 parts of Haematococcus pluvialis, 10 parts of walnut kernel extract, 10 parts of Cordyceps militaris extract, 0.5 part of rice bran alkanol.

[0055] Comparative Example 1

[0056] Compared with Example 1, it only contains Bacillus subtilis fibrinolytic enzyme.

[0057] Raw material composition: Bacillus subtilis fibrinolytic enzyme.

[0058] Comparative Example 2

[0059] Compared with Example 1, it does not contain Bacillus subtilis fibrinolytic enzyme, and the other component ratios remain unchanged.

[0060] Raw material composition (parts by weight): 15 parts of Haematococcus pluvialis, 10 parts of walnut kernel extract, 10 parts of Cordyceps militaris extract, 0.5 part of rice bran alkanol.

[0061] Experimental Example 1 Test for spermatogenic dysfunction of kidney-yang deficiency type

[0062] Establishment of kidney-yang deficiency rat model: Rats were intramuscularly injected with hydrocortisone injection at a dose of 10 mg / rat per day for 2 consecutive weeks to establish a kidney-yang deficiency model. After modeling, the rats showed symptoms such as fear of cold, preference for warmth, cold extremities, clear and long urine, curling up, dull and scattered hair, listlessness, and emaciation, which met the symptoms of kidney-yang deficiency, indicating successful modeling.

[0063] Animal grouping and administration method: Normal rats were used as the blank control group, the previously obtained kidney-yang deficiency rat model was used as the model group, 3 experimental groups and 2 control groups, with 20 rats in each group. The experimental groups were respectively administered the compositions for improving male reproductive ability prepared in Examples 1-3, and the control groups were respectively administered the compositions for improving male reproductive ability prepared in Comparative Examples 1-2. The dosage of each administration was set at 1.5 g / Kg, twice a day, for 4 consecutive weeks. The blank control group and the model group were not administered. After 4 weeks, the wet weights of the left testis, spleen, and thymus of each group of animals were measured; the levels of serum testosterone (T), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were detected; the sperm motility percentage and sperm density were measured.

[0064] Results: (1) The results of the effects on the wet weights of the left testis, spleen, and thymus of the model rats are shown in Table 2.

[0065] Table 2 Results of the determination of the wet weights of the left testis, spleen, and thymus of rats in each group

[0066]

[0067]

[0068] Note: *P < 0.05, **P < 0.01 vs the model group

[0069] As can be seen from Table 2, after 2 weeks of drug administration treatment for the model rats, the wet weights of the left testis, spleen, and thymus in each drug administration group showed an upward trend to varying degrees compared with the model group. Among them, the wet weights of the left testis and spleen in the experimental group of the composition increased significantly. The results indicate that the compositions for improving male reproductive ability and subtilisin in Examples 1 - 3 are all beneficial to the recovery of the reproductive organs of the kidney-yang deficiency rats. The effect of the compositions in Examples 1 - 3 is better than that of subtilisin of Bacillus subtilis in Comparative Example 1, indicating the synergistic effect of each raw material.

[0070] Results: (2) The results of the effects on the serum T, LH, and FSH levels of the model rats are shown in Table 3.

[0071] Table 3 Results of the serum T, LH, and FSH levels of rats in each group

[0072] Group T (ng / dL) LH (mIU / ml) FSH (mIU / ml) Control group 28.96±3.63 9.36±2.24 11.26±2.09 Model group 20.27±3.58 6.68±2.17 8.13±1.75 Comparative example 1 group 23.68±3.26 8.27±2.06 <![CDATA[9.86±2.13 * > Comparative example 2 group 20.88±3.42 7.06±1.87 8.47±1.59 Example 1 group <![CDATA[26.77±3.86 * > <![CDATA[8.85±1.89 * > <![CDATA[10.24±1.78 * > Example 2 group 25.64±3.56 8.52±1.63 10.03±1.42 Example 3 group <![CDATA[27.32±3.47 * > <![CDATA[9.05±1.91 ** > <![CDATA[10.88±1.86 * >

[0073] Note: *P < 0.05, **P < 0.01 vs the model group.

[0074] As can be seen from the results in Table 3, compared with the animals in the model group, the serum T, LH, and FSH levels in each group of rats showed an increasing trend. Among them, the FSH content in the experimental group of subtilisin of Bacillus subtilis increased significantly, and the T, LH, and FSH contents in the experimental group of the composition all increased significantly. The results indicate that the synergistic effect of the composition in Example 3 has a significant effect on the production of sex hormones in kidney-yang deficiency rats.

[0075] Results: (3) The results of the effects on the sperm motility rate and sperm density of the model rats are shown in Table 4.

[0076] Table 4 Results of the sperm motility rate and sperm density of rats in each group

[0077] Group Sperm motility rate (%) <![CDATA[Sperm density (*10 6 / ml)]]> Control group 86.42±8.09 74.12±9.21 Model group 30.58±7.22 23.74±7.34 Comparative example 1 group 67.52±6.83 58.55±9.82 Comparative example 2 group 32.46±6.58 22.84±6.65 Example 1 group 82.21±6.82 71.23±8.36* Example 2 group 79.35±7.54 70.57±8.65 Example 3 group 83.66±7.79* 72.31±9.05**

[0078] Note: *P < 0.05, **P < 0.01 vs the model group.

[0079] As can be seen from the results in Table 4, the sperm motility rate and sperm density of rats in each group were higher than those of the rats in the model group, and the sperm motility rate and sperm density of the rats in the experimental group of the composition were significantly increased, approaching the level of the control group. The results show that the compositions of Examples 1-3 have a better effect than Bacillus subtilis fibrinolytic enzyme of Comparative Example 1, indicating that the synergistic effect has a significant effect on increasing the sperm count and motility rate of male rats with kidney yang deficiency.

[0080] Experiment Example 2: Test on the Genital Development of Immature Male Rats

[0081] Take 2-month-old S.D male rats with a body weight of 75-85 g. They were divided into 2 groups (20 rats in each group). The control group was fed normally, the experimental group was continuously fed the composition prepared in Examples 1-3 for 2 weeks, and the comparison group was continuously fed the composition prepared in Comparative Examples 1-2 for 2 weeks. The dosage of each administration was set at 0.7 g / Kg, twice a day. After 2 weeks, the animals were sacrificed, and the wet weights of the testis, epididymis, adenohypophysis, and seminal vesicle were weighed.

[0082] Results: The results of the influence of the composition for improving male reproductive ability on the genital development of immature male rats are shown in Table 5.

[0083] Table 5 Results of Genital Development of Male Rats

[0084]

[0085] Note: *P<0.05, **P<0.01 vs the control group.

[0086] As can be seen from the results in Table 5, compared with the control group, the composition for improving male reproductive ability can accelerate the development of the testis, epididymis, adenohypophysis, and seminal vesicle of the genital organs of immature male rats. The results show that the synergistic effect of the compositions in Examples 1-3 has a significant promoting effect on the development of the testis, epididymis, and seminal vesicle of immature male rats.

[0087] Conclusion: Bacillus subtilis fibrinolytic enzyme has a positive effect on improving the recovery of reproductive organs, androgen production, and sperm survival rate of male rats with kidney yang deficiency, and can also promote the development of the reproductive organs of immature male rats. The composition of the five substances of Bacillus subtilis fibrinolytic enzyme, Haematococcus pluvialis, walnut kernel extract, Cordyceps militaris extract, and rice bran alkanol can synergistically enhance the effect, significantly improve the reproductive ability of male rats with kidney yang deficiency, promote the development of the reproductive organs of immature male rats, and has good application prospects in the development of functional products for improving male reproductive ability.

[0088] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A composition for improving male reproductive ability, characterized in that, It is prepared from the following raw materials in parts by weight: 10-30 parts of Bacillus subtilis fibrinolytic enzyme, 5-15 parts of Haematococcus pluvialis, 3-10 parts of walnut kernel extract, 3-10 parts of Cordyceps militaris extract, 0.3-0.5 parts of rice bran alkanol; the Bacillus subtilis fibrinolytic enzyme is fermented from the strain Bacillus subtilis QK02, and the strain is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO:M 203078; The preparation method of the Bacillus subtilis fibrinolytic enzyme is as follows: S1. Seed culture preparation; The strain Bacillus subtilis QK02 is stored in a -80°C low-temperature refrigerator. Before use, the strain is activated on a fresh slant for 18 - 24 h. One loop of cells is picked from the slant and inoculated into a triangular flask containing LB liquid medium, and cultured on a shaker at 35 - 40°C and 100 - 500 r / min for 15 - 20 h; S2. Fermentation culture: Sterilize the fermentation medium at 110 - 115 °C for 10 - 30 min. After cooling to room temperature, inoculate the above seed liquid into the fermentation medium at an inoculation amount of 1%, and start fermentation for 20 - 30 h; among them, control the temperature at 35 - 40 °C, humidity at 80 - 90%, and dissolved oxygen at 20 - 40% in the fermentation equipment; S3. Post-treatment: Centrifuge the obtained fermentation broth with a tubular centrifuge, concentrate it by microfiltration and ultrafiltration to obtain a concentrated solution, and spray-dry the concentrated solution at an inlet air temperature of 120 - 150 °C and an outlet air temperature of 63 - 68 °C to obtain Bacillus subtilis fibrinolytic enzyme; The Haematococcus pluvialis is obtained by the following method: Using the Haematococcus pluvialis algal bodies and spores as raw materials, stir and break the cell walls with a high-speed dispersion stirrer at 400 - 800 revolutions per minute, and dry it with hot air at 60 - 80 °C until the water content is below 10% to obtain Haematococcus pluvialis powder; The walnut extract is obtained by the following method: Crush walnuts, add an ethanol solution with a volume fraction of 85% according to a material-liquid ratio of 1:10, reflux and extract at 60 °C for 2 - 3 times, with each extraction time of 2 - 4 h, filter while it is hot, combine the filtrates, and concentrate under reduced pressure to obtain an extract without alcohol smell, which is the walnut extract; The Cordyceps militaris extract is obtained by the following method: Crush Cordyceps militaris, add 8 - 20 times the weight of water, extract at 80 - 105 °C for 1 - 3 times, extract for 2 - 6 h, filter the extract, combine them, and concentrate to a solid content of 5% - 25% to obtain a concentrated Cordyceps militaris extract. Dry the concentrated Cordyceps militaris extract to obtain the Cordyceps militaris extract.

2. The composition for improving male reproductive ability according to claim 1, wherein The Bacillus subtilis fibrinolytic enzyme is obtained by fermenting the strain Bacillus subtilis QK02, followed by centrifugation, concentration, and drying.

3. The composition for improving male reproductive ability according to claim 1, wherein The Cordyceps militaris extract contains cordycepin.

4. The composition for improving male reproductive ability according to claim 1, wherein, The Haematococcus pluvialis contains astaxanthin.

5. The composition for improving male reproductive ability according to claim 1, characterized in that, The rice bran aliphatic alcohol contains octacosanol.

6. The composition for improving male reproductive ability according to claim 1, characterized in that, The composition further includes acceptable excipients.

7. A method for preparing a composition for improving male reproductive ability according to any one of claims 1-6, characterized in that, Mix the Bacillus subtilis fibrinolytic enzyme, Haematococcus pluvialis, walnut extract, Cordyceps militaris extract, and rice bran aliphatic alcohol in proportion to obtain a composition for improving male reproductive ability.

8. Use of a composition for improving male reproductive ability according to any one of claims 1 - 6 in the preparation of a drug for improving male reproductive ability.

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