Ep tube rack for RNA extraction

Abstract

The application belongs to the technical field of RNA extraction devices, and particularly relates to an ep tube rack for RNA extraction, which comprises an ep tube rack body, a controller, a placing hole, a sucking assembly and a shaking assembly. A plurality of rows of placing holes with different specifications are formed in the ep tube rack body. The sucking assembly comprises a clamping ring, a fixing frame, a fixing column and a stabilizing frame. The fixing frame is fixed to one side of the front end of the ep tube rack body. The fixing columns are rotatably connected to the adjacent surfaces on the upper ends of the two fixing frames, and the fixing columns are damping shafts. The clamping ring is fixed between the two fixing columns. The stabilizing frame is fixed to the middle of the front end of the ep tube rack body. The application solves the problem that if the experimenter shakes hands, the upper layer of colorless water phase is easily transferred to a new ep tube after centrifugation, which can cause experimental failure.

Description

Technical Field

[0001] This invention belongs to the technical field of RNA extraction devices, specifically an EP tube rack for RNA extraction. Background Technology

[0002] Eppendorf tubes are containers used to hold samples in biological experiments. Because the bottom of Eppendorf tubes is uneven, they cannot be placed directly on a table, so a stand is needed to support them. Such a stand is called an Eppendorf tube stand.

[0003] For example, a Chinese patent with application number CN201820733252.9 discloses an EP tube rack for RNA extraction, including an EP tube rack body. The upper surface of the EP tube rack body is engraved with an upper row number and a side row number. The upper surface of the EP tube rack body is vertically engraved with a septum line. The EP tube rack body is divided into an ice box area and a room temperature area. The ice box area is spaced apart with inclined EP tube placement holes and vertical EP tube placement holes. The room temperature area is also horizontally opened with a placement groove and is also provided with a room temperature vertical EP placement hole.

[0004] Although this technical solution makes operation easier by setting up inclined placement holes and ice box areas to prevent movement of liquid phase surfaces in the EP tube and providing a low-temperature operating area, there is a risk of failure when transferring the upper colorless aqueous phase from the EP tube to a new EP tube after centrifugation. If the experimenter shakes their hand or bumps into other objects during the transfer process, the transfer of the colorless aqueous phase may fail, leading to experimental failure.

[0005] Based on this, the present invention designs an ep tube holder for RNA extraction to solve the technical problems existing in the prior art. Summary of the Invention

[0006] To overcome the shortcomings of existing technologies and solve the problem that experimental failures can easily occur if the experimenter shakes their hand when transferring the upper colorless aqueous phase into a new EP tube after centrifugation, this invention proposes an EP tube rack for RNA extraction.

[0007] The technical solution adopted by the present invention to solve its technical problem is as follows: An EP tube holder for RNA extraction, comprising an EP tube holder body, a controller, placement holes, an aspiration assembly, and a mixing assembly; the EP tube holder body has multiple rows of placement holes of different specifications; the aspiration assembly includes a clamping ring, a fixing frame, a fixing post, and a stabilizing frame; a fixing frame is fixedly connected to one side of the front end of the EP tube holder body; two fixing posts are rotatably connected to two adjacent surfaces at the upper ends of the two fixing frames, and the fixing posts are damping shafts; a clamping ring is fixedly connected between the two fixing posts; a stabilizing frame is fixedly connected to the middle of the front end of the EP tube holder body.

[0008] During the RNA extraction process, after centrifugation, the sample will separate into three layers. The upper colorless aqueous phase needs to be transferred to a new EP tube. During this process, the intermediate phase must not come into contact with the sample. If the experimenter's hand shakes during the operation, the experiment will fail.

[0009] Therefore, this invention features an aspiration assembly; after centrifugation, the sample is placed into a clamping ring, which is tilted, so the EP tube is also tilted; the experimenter then picks up the sampler and tilts it into the EP tube at the same angle; once the sampler is in the upper aqueous phase, the experimenter rests their hand on a stabilizing frame and uses the sampler to remove the upper aqueous phase and transfer it to a new EP tube, which is then placed in the placement hole; when a new solution is added and needs to be mixed, the mixing assembly is used to mix the solution in the EP tube.

[0010] This invention features a clamping ring and a stabilizing frame. The clamping ring secures the EP tube containing the sample, preventing experimental failures caused by shaky hands when handling the sample. The stabilizing frame provides support for the experimenter's hand when transferring the upper aqueous phase, reducing the likelihood of hand tremors during the aspiration of the upper aqueous phase. This improves the success rate of the experiment and enhances the functionality of the EP tube rack.

[0011] Preferably, the shaking assembly includes a small motor, a pressing plate, and a hinged column; the small motor is fixedly connected to the front-end fixed frame, and the rear end of the small motor passes through the fixed frame and is fixedly connected to the fixed column, with the fixed column rotatably connected to the fixed frame; the pressing plate engages with the EP tube cap, and the end of the pressing plate facing the clamping ring has a groove matching the shape of the EP tube cap; the front end of the pressing plate is fixedly connected to one end of the hinged column; the other end of the hinged column is hinged to the front-end fixed column, and the hinged column is a telescopic rod; during operation, the solution in the sample needs to be inverted multiple times to mix evenly during the experiment, enhancing the experimenter's... The workload is reduced; therefore, this invention incorporates a shaking assembly. When a new solution is added and shaking is required, the operator places the EP tube cap on and inserts it into the clamping ring. Then, the operator picks up the pressing plate and stretches the hinge column, causing the hinge column to rotate around the fixed column. This rotates the pressing plate to the position of the EP tube. The pressing plate is then pressed towards the EP tube cap, causing the hinge column to retract and engage with the EP tube cap in the groove within the pressing plate. The controller then controls a small motor to drive the fixed column to rotate back and forth, causing the clamping ring and EP tube to rotate back and forth, thus mixing the solution in the EP tube evenly. This further enhances the functionality of the EP tube holder.

[0012] Preferably, the clamping ring is further fitted with a plurality of clamping rings of progressively smaller size; the upper end of the inner clamping ring is fixedly connected to a sleeve plate; during operation, by setting the sleeve plate, when a smaller EP tube is to be used, the smaller clamping ring is manually inserted along the inner wall of the outer ring clamping ring by holding the sleeve plate, and the sleeve plate fixes the inner ring clamping ring on the outer ring clamping ring; thus, by moving the sleeve plate, clamping rings of different sizes can be moved, thereby changing the size of the clamping ring used; the sleeve plate is slidably connected to the outer ring clamping ring; thus expanding the application range of the EP tube rack.

[0013] Preferably, the stabilizer is configured as an arc shape with a gradually decreasing slope from bottom to top; a groove is provided in the middle of the upper end of the stabilizer; sliding pads are fixedly connected to both ends of the groove on the stabilizer; during operation, the present invention reduces the contact area between the stabilizer and the experimenter's hand by setting the groove, thereby reducing the friction between the stabilizer and the experimenter's hand; the sliding pads are made of smooth TPE material, and the setting of the sliding pads also reduces friction; making it smoother for the experimenter to move the sampler; and enhancing the use effect of the EP tube rack.

[0014] Preferably, a telescopic rod is fixedly connected to the rear end of the pressing plate, and a locking block is fixedly connected to one end of the telescopic rod facing the fixing column; a locking groove is provided on the fixing column at the rear end; during operation, by setting the locking block and the locking groove, when the pressing plate rotates to the position corresponding to the EP tube cover, the pressing plate is pressed towards the EP tube cover, so that the locking block and the locking groove engage; thus, when using EP tubes of different specifications, the groove inside the pressing plate cannot engage with each specification of EP tube; therefore, by engaging the locking block on the pressing plate with the locking groove on the fixing column, the pressing plate can fix EP tubes of different specifications placed on the clamping ring; further improving the application range of the EP tube rack.

[0015] Preferably, a ball bearing is provided inside the sleeve plate at the position where it contacts the outer ring clamping ring; anti-slip cotton is fixedly connected inside the sleeve plate at the position adjacent to the ball bearing; during operation, the present invention, by setting the ball bearing, transforms the sliding friction between the sleeve plate and the outer ring clamping ring into rolling friction, thereby reducing the friction force when moving the sleeve plate and making the movement of the sleeve plate smoother; the anti-slip cotton is provided to hold the two ends of the ball bearing, so that the sleeve plate does not rotate around the outer ring clamping ring with the ball bearing, ensuring the stability of the inner ring clamping ring; the user experience of using the EP tube rack is optimized.

[0016] The beneficial effects of this invention are as follows:

[0017] 1. The EP tube holder for RNA extraction described in this invention, compared with traditional technical solutions, features a clamping ring and a stabilizing frame. The clamping ring secures the EP tube containing the sample, preventing experimental failure caused by hand tremors when handling the sample EP tube. The stabilizing frame provides support for the experimenter's hand when transferring the upper aqueous phase, reducing the possibility of hand tremors when aspirating the upper aqueous phase. This improves the success rate of the experiment and enhances the functionality of the EP tube holder.

[0018] 2. The EP tube holder for RNA extraction described in this invention features a shaking component. When a new solution is added and shaking is required, the operator places the EP tube cap on and inserts it into the clamping ring. Then, the operator lifts the pressing plate and stretches the hinge column, causing the hinge column to rotate around the fixed column. This rotates the pressing plate to the position of the EP tube. The operator then presses the pressing plate towards the EP tube cap, causing the hinge column to retract and the groove in the pressing plate to engage with the EP tube cap. This further enhances the functionality of the EP tube holder. Attached Figure Description

[0019] The invention will now be further described with reference to the accompanying drawings.

[0020] Figure 1 This is a perspective view of the present invention;

[0021] Figure 2 This is an exploded view of the suction component and the shaking component of the present invention;

[0022] In the diagram: 1. EP tube frame body; 2. Placement hole; 3. Suction assembly; 31. Clamping ring; 32. Fixing frame; 33. Fixing column; 34. Stabilizing frame; 4. Shaking assembly; 41. Small motor; 42. Pressing plate; 43. Hinge column; 5. Sleeve plate; 6. Groove; 7. Sliding pad; 8. Locking block; 9. Locking slot; 10. Ball bearing; 11. Anti-slip cotton. Detailed Implementation

[0023] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0024] Example 1:

[0025] like Figures 1 to 2As shown, the present invention discloses an EP tube holder for RNA extraction, comprising an EP tube holder body 1, a controller, placement holes 2, an aspiration assembly 3, and a shaking assembly 4; the EP tube holder body 1 has multiple rows of placement holes 2 of different sizes; the aspiration assembly 3 includes a clamping ring 31, a fixing frame 32, a fixing post 33, and a stabilizing frame 34; a fixing frame 32 is fixedly connected to one side of the front end of the EP tube holder body 1; two adjacent surfaces at the upper ends of the two fixing frames 32 are rotatably connected to fixing posts 33, the fixing posts 33 being damping shafts; a clamping ring 31 is fixedly connected between the two fixing posts 33; and a stabilizing frame 34 is fixedly connected to the middle of the front end of the EP tube holder body 1.

[0026] In this embodiment, during RNA extraction, after centrifugation, the sample will separate into three layers. The upper colorless aqueous phase needs to be transferred to a new EP tube. During this process, the intermediate phase must not come into contact with the sample. If the experimenter's hand shakes during the operation, the experiment will fail.

[0027] Therefore, this invention uses an aspiration component 3; after centrifugation, the sample is placed into a clamping ring 31, which is tilted, so the EP tube is also tilted; the experimenter then picks up the sampler and tilts it into the EP tube along its tilt angle; after the sampler is inserted into the upper aqueous phase, the experimenter rests their hand on a stabilizing frame 34 and uses the sampler to remove the upper aqueous phase and transfer it to a new EP tube, which is placed in the placement hole 2; when a new solution is added and needs to be mixed, the shaking component 4 is used to shake the solution in the EP tube.

[0028] The present invention incorporates a clamping ring 31 and a stabilizing frame 34. The clamping ring 31 secures the EP tube containing the sample, preventing experimental failure caused by hand tremors when the experimenter handles the sample EP tube. The stabilizing frame 34 provides support for the experimenter's hand when transferring the upper aqueous phase, reducing the possibility of hand tremors when the experimenter draws up the upper aqueous phase. This improves the success rate of the experiment and enhances the functionality of the EP tube rack.

[0029] Simultaneously, the shaking assembly 4 includes a small motor 41, a pressing plate 42, and a hinged column 43. The small motor 41 is fixedly connected to the front-end fixing frame 32, and the rear end of the small motor 41 passes through the fixing frame 32 and is fixedly connected to the fixing column 33. The fixing column 33 is rotatably connected to the fixing frame 32. The pressing plate 42 engages with the EP tube cap. One end of the pressing plate 42 facing the clamping ring 31 has a groove that matches the shape of the EP tube cap. The front end of the pressing plate 42 is fixedly connected to one end of the hinged column 43. The other end of the hinged column 43 is hinged to the front-end fixing column 33, and the hinged column 43 is a telescopic rod. During operation, the solution in the sample needs to be inverted multiple times to mix evenly during the experiment, which enhances the experimental efficiency. To reduce the workload of personnel, this invention incorporates a shaking assembly 4. When a new solution is added and shaking is required, the operator places the EP tube cap on and into the clamping ring 31. Then, the operator picks up the pressing plate 42 and stretches the hinge column 43, causing the hinge column 43 to rotate around the fixed column 33. This causes the pressing plate 42 to rotate to the position of the EP tube. The operator then presses the pressing plate 42 towards the EP tube cap, causing the hinge column 43 to retract and engage with the EP tube cap in the groove of the pressing plate 42. The controller then controls the small motor 41 to drive the fixed column 33 to rotate back and forth, causing the fixed column 33 to drive the clamping ring 31 and the EP tube to rotate back and forth, thus mixing the solution in the EP tube evenly. This further enhances the functionality of the EP tube holder.

[0030] Example 2:

[0031] like Figure 1 As shown, a plurality of clamping rings 31 with successively decreasing sizes are also sleeved inside the clamping ring 31, and a sleeve plate 5 is fixedly connected to the upper end of the internal clamping ring 31.

[0032] In this embodiment, by setting the sleeve plate 5, when a smaller EP tube is to be used, the sleeve plate 5 is manually held and the smaller clamping ring 31 is inserted along the inner wall of the outer ring clamping ring 31. The sleeve plate 5 fixes the inner ring clamping ring 31 on the outer ring clamping ring 31. This allows the clamping rings 31 of different sizes to be moved by moving the sleeve plate 5, thereby changing the size of the clamping ring 31 used. The sleeve plate 5 is slidably connected to the outer ring clamping ring 31, thereby expanding the application range of the EP tube rack.

[0033] Meanwhile, the stabilizer 34 is designed as an arc with a gradually decreasing slope from bottom to top, and a groove 6 is provided in the middle of the upper end of the stabilizer 34; sliding pads 7 are fixed to both ends of the stabilizer 34 at the groove 6. By setting the groove 6, the contact area between the stabilizer 34 and the experimenter's hand is reduced, thereby reducing the friction between the stabilizer 34 and the experimenter's hand. At the same time, the sliding pads 7 are made of smooth TPE material, and the setting of the sliding pads 7 also reduces the friction, making it smoother for the experimenter to move the sampler, thus enhancing the use effect of the EP tube rack.

[0034] Example 3:

[0035] like Figure 2 As shown, a telescopic rod is fixedly connected to the rear end of the pressing plate 42, and a locking block 8 is fixedly connected to one end of the telescopic rod facing the fixing post 33; a locking groove 9 is provided on the fixing post 33 at the rear end.

[0036] In this embodiment, by setting the locking block 8 and the locking groove 9, when the pressing plate 42 is rotated to the position corresponding to the EP tube cover, the pressing plate 42 is pressed towards the EP tube cover, so that the locking block 8 and the locking groove 9 are engaged; thus, when using EP tubes of different specifications, the groove inside the pressing plate 42 cannot engage with each specification of EP tube; therefore, by engaging the locking block 8 on the pressing plate 42 with the locking groove 9 on the fixing post 33, the pressing plate 42 can fix EP tubes of different specifications when they are placed on the clamping ring 31, further improving the application range of the EP tube rack.

[0037] Example 3:

[0038] like Figure 2 As shown, a ball bearing 10 is provided inside the sleeve plate 5 at the position where it contacts the outer ring clamping ring 31, and an anti-slip cotton 11 is fixedly connected inside the sleeve plate 5 at the position adjacent to the ball bearing 10.

[0039] In this embodiment, by setting the ball bearing 10, the sliding friction between the sleeve plate 5 and the outer ring clamping ring 31 is transformed into rolling friction, thereby reducing the friction force when moving the sleeve plate 5 and making the movement of the sleeve plate 5 smoother. By setting the anti-slip cotton 11 to hold the two ends of the ball bearing 10, the sleeve plate 5 will not rotate around the outer ring clamping ring 31 with the ball bearing 10, ensuring the stability of the inner ring clamping ring 31 and optimizing the user experience of using the EP tube rack.

Claims

1. An EP tube holder for RNA extraction, comprising an EP tube holder body, characterized in that: The EP tube rack also includes a controller, placement holes, a suction assembly, and a shaking assembly; the EP tube rack body has multiple rows of placement holes of different specifications; the suction assembly includes a clamping ring, a fixing frame, a fixing post, and a stabilizing frame; a fixing frame is fixedly connected to one side of the front end of the EP tube rack body; two fixing posts are rotatably connected to two adjacent surfaces at the upper ends of the two fixing frames, and the fixing posts are damping shafts; a clamping ring is fixedly connected between the two fixing posts; a stabilizing frame is fixedly connected to the middle of the front end of the EP tube rack body. The shaking assembly includes a small motor, a pressing plate, and a hinged column. The small motor is fixedly connected to a front-end fixed frame, and the rear end of the small motor passes through the fixed frame and is fixedly connected to the fixed column. The fixed column is rotatably connected to the fixed frame. The pressing plate engages with the EP tube cap. One end of the pressing plate facing the clamping ring has a groove that matches the shape of the EP tube cap. The front end of the pressing plate is fixedly connected to one end of the hinged column. The other end of the hinged column is hinged to the front-end fixed column, and the hinged column is a telescopic rod. The clamping ring is further fitted with a plurality of clamping rings of progressively smaller size; the upper end of the internal clamping ring is fixedly connected to a sleeve plate. When using a smaller EP tube, manually hold the sleeve plate and insert the smaller clamping ring along the inner wall of the outer clamping ring. The sleeve plate fixes the inner clamping ring on the outer clamping ring. This allows the clamping ring of different sizes to be moved by moving the sleeve plate, thereby changing the size of the clamping ring used. A telescopic rod is fixedly connected to the rear end of the pressing plate, and a locking block is fixedly connected to one end of the telescopic rod facing the fixed post; a locking groove is provided on the fixed post at the rear end. The sleeve plate is provided with a ball bearing at the position where it contacts the outer ring clamping ring; anti-slip cotton is fixedly connected to the sleeve plate at the position adjacent to the ball bearing. The stabilizer is configured as an arc with a gradually decreasing slope from bottom to top; a groove is provided in the middle of the upper end of the stabilizer; and sliding pads are fixedly connected to both ends of the groove on the stabilizer.

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