Liquid path system of immunoluminescence analyzer and immunoluminescence analyzer

Abstract

The present invention relates to a liquid circuit system of a chemiluminescence immunoassay instrument, comprising: a fluid replenishment module, a first cleaning module, a second cleaning module, a waste liquid module and an excitation liquid module, wherein the fluid replenishment module comprises a system liquid branch and a cleaning liquid branch; the system liquid branch is connected to the first cleaning module, and the system liquid branch is controlled by a controller to provide system liquid for cleaning a sample needle and a reagent needle; the cleaning liquid branch is connected to the second cleaning module, and the controller controls the cleaning liquid branch to provide cleaning liquid for the liquid addition needle, and the cleaning liquid is used to clean the reagent in the reaction tube; the waste liquid module is respectively connected to the first cleaning module, the second cleaning module and the reagent compartment, and the controller controls the waste liquid module to receive the waste liquid generated by the first cleaning module, the second cleaning module and the reagent compartment; the excitation liquid module, and the controller controls the excitation liquid module to provide excitation liquid for the chemiluminescence instrument to excite a luminescent substance; the liquid circuit system also includes: a special cleaning liquid module. The liquid circuit system provided in the embodiment of the present application is used for a chemiluminescence immunoassay instrument, and the liquid circuit system can perform multiple actions simultaneously, saving working time.

Description

Technical Field

[0001] The present invention relates to the technical field of medical detection equipment, in particular to a liquid path system of an immunoluminescence analyzer and the immunoluminescence analyzer. Background Art

[0002] The chemiluminescence immunoassay instrument is a medical testing instrument that performs immunoassay on the human body by testing the patient's serum. It mainly includes: a liquid adding arm, a cleaning tray, a sample library, an incubation tray, a reagent compartment, a detection tray, etc. The liquid adding arm in the chemiluminescence immunoassay instrument is divided into a sample needle liquid adding arm and a reagent needle liquid adding arm. The sample needle and the reagent needle need to be cleaned in time after each use. The cleaning tray needs to clean the reaction tube. The detection tray needs to add excitation liquid to the reaction tube, and the used waste liquid needs to be discharged in time. To this end, the liquid path system in the chemiluminescence immunoassay instrument needs to meet the use requirements of each functional unit, and the liquid path system can perform multiple actions at the same time so that the operation of the liquid path system in each functional unit is not affected. Summary of the Invention

[0003] In response to the technical problems existing in the prior art, the present invention proposes a fluid path system and an immunoluminescence analyzer for an immunoluminescence analyzer. By setting up multiple fluid path modules, the usage requirements of each functional unit in the chemiluminescence immunoassay are met according to the corresponding requirements of each fluid path module. In addition, a controller can control the working process of each fluid path module in the fluid path system.

[0004] An embodiment of the present application proposes a liquid circuit system of a chemiluminescence immunoassay instrument, comprising: a fluid replenishment module, a first cleaning module, a second cleaning module, a waste liquid module and an excitation liquid module, wherein the fluid replenishment module comprises a system liquid branch and a cleaning liquid branch; the system liquid branch is connected to the first cleaning module, and the system liquid branch is controlled by a controller to provide system liquid for cleaning the sample needle and the reagent needle; the cleaning liquid branch is connected to the second cleaning module, and the controller controls the cleaning liquid branch to provide cleaning liquid for the liquid addition needle, and the cleaning liquid is used to clean the reagent in the reaction tube; the waste liquid module is respectively connected to the first cleaning module, the second cleaning module and the reagent tank, and the controller controls the waste liquid module to receive the waste liquid generated by the first cleaning module, the second cleaning module and the reagent tank; the excitation liquid module, and the controller controls the excitation liquid module to provide excitation liquid for the chemiluminescence analyzer, which is used to excite the luminescent substance; the liquid circuit system also includes: a special cleaning liquid module.

[0005] Optionally, the special cleaning fluid module is connected to the first cleaning module, and the controller controls the special cleaning fluid module to provide special cleaning fluid for cleaning the sample needle and / or the reagent needle.

[0006] Optionally, the system liquid branch includes: a system liquid storage barrel, a first liquid level sensor is provided in the system liquid storage barrel, the first liquid level sensor is connected to the controller, the first liquid level sensor is used to monitor the liquid level in the system liquid storage barrel, and output a liquid level monitoring signal to the controller.

[0007] Optionally, the system liquid branch also includes: a system liquid buffer tank, which is connected to the system liquid storage tank through the first pump body, and a second liquid level sensor is provided in the system liquid storage tank. The controller is electrically connected to the first pump body and the second liquid level sensor, and the second liquid level sensor includes a second high-level float and a second low-level float. The second high-level float is used for fluid replenishment monitoring, and the second low-level float is used for alarm monitoring.

[0008] Optionally, the cleaning liquid branch includes: a cleaning liquid storage barrel, a third liquid level sensor is provided in the cleaning liquid storage barrel, the third liquid level sensor is connected to the controller, the third liquid level sensor is used to monitor the liquid level in the cleaning liquid storage barrel, and output a liquid level monitoring signal to the controller.

[0009] Optionally, the cleaning fluid branch also includes: a cleaning fluid buffer barrel, which is connected to the cleaning fluid storage barrel through the second pump body, a fourth liquid level sensor is provided in the cleaning fluid buffer barrel, the controller is electrically connected to the second pump body and the fourth liquid level sensor, the fourth liquid level sensor includes a fourth high-level float and a fourth low-level float, the fourth high-level float is used for fluid replenishment monitoring, and the fourth low-level float is used for alarm monitoring.

[0010] Optionally, the first cleaning module includes: an external cleaning branch and an internal cleaning branch; the external cleaning branch includes: at least one first diaphragm pump, a first solenoid valve group and a liquid supply pipeline whose first end liquid outlet is connected to the sample cleaning tank or the reagent cleaning tank; the internal cleaning branch includes: at least one second diaphragm pump, a second solenoid valve group, at least one first plunger pump and a liquid supply pipeline whose second end liquid outlet is connected to the inside of the sample needle or the reagent needle; the controller controls the liquid supply of the external cleaning branch and the internal cleaning branch separately or simultaneously.

[0011] Optionally, the second cleaning module includes: multiple cleaning branches whose ends are connected to the liquid adding needles, each cleaning branch includes at least one second plunger pump and a corresponding solenoid valve, and the controller controls the multiple cleaning branches to supply liquid for cleaning in a predetermined order.

[0012] Optionally, the waste liquid module includes a first cleaning module waste liquid branch, a second cleaning module waste liquid branch, a reagent tank waste liquid branch and a waste liquid barrel, wherein the first cleaning module waste liquid branch includes at least one third pump body, the third pump body is connected to the controller, and the controller controls the third pump body to extract the waste liquid generated by the first cleaning module into the waste liquid barrel; the second cleaning module waste liquid branch includes a peristaltic pump, the peristaltic pump input end is connected to the pipette needle, the peristaltic pump is connected to the controller, and the controller controls the peristaltic pump to extract the waste liquid generated by the second cleaning module into the waste liquid barrel; the reagent tank branch outlet is connected to the waste liquid barrel; a fifth liquid level sensor is provided in the waste liquid barrel, and the fifth liquid level sensor is used to monitor the liquid level in the waste liquid barrel and output a liquid level monitoring signal to the controller.

[0013] Optionally, the waste liquid module includes a first cleaning module waste liquid branch, a second cleaning module waste liquid branch, a reagent tank waste liquid branch, a waste liquid barrel and a waste liquid buffer barrel, wherein the first cleaning module waste liquid branch includes at least one third pump body, the third pump body is connected to the controller, and the controller controls the third pump body to extract the waste liquid generated by the first cleaning module into the waste liquid buffer barrel; the second cleaning module waste liquid branch includes a peristaltic pump, the peristaltic pump input end is connected to the pipette needle, the peristaltic pump is connected to the controller, and the controller controls the peristaltic pump to extract the waste liquid generated by the second cleaning module into the waste liquid buffer barrel; the reagent tank branch outlet is connected to the waste liquid buffer barrel; a sixth liquid level sensor is provided in the waste liquid buffer barrel, the sixth liquid level sensor is connected to the controller, and is used to monitor the liquid level in the waste liquid buffer barrel; a fifth liquid level sensor is provided in the waste liquid barrel, the waste liquid barrel is connected to the waste liquid buffer barrel through the fourth pump body, the fifth liquid level sensor and the fourth pump body are connected to the controller, and the fifth liquid level sensor is used to detect the liquid level in the waste liquid barrel.

[0014] Optionally, the waste liquid module includes a first cleaning module waste liquid branch, a second cleaning module waste liquid branch, a reagent tank waste liquid branch, a waste liquid buffer barrel and a sewer discharge branch, wherein the first cleaning module waste liquid branch includes at least one third pump body, at least one third pump body is connected to the controller, and the controller controls the at least one third pump body to extract the waste liquid generated by the first cleaning module into the waste liquid buffer barrel; the second cleaning module waste liquid branch includes a peristaltic pump, the peristaltic pump input end is connected to the pipette needle, the peristaltic pump is connected to the controller, and the controller controls the peristaltic pump to extract the waste liquid generated by the second cleaning module into the waste liquid buffer barrel; the reagent tank waste liquid branch is connected to the waste liquid buffer barrel; a sixth liquid level sensor is provided in the waste liquid buffer barrel, and the sixth liquid level sensor is used to monitor the liquid level in the waste liquid buffer barrel and output a liquid level monitoring signal to the controller; the sewer discharge branch is connected to the waste liquid buffer barrel through the fifth pump body, and is used to discharge the waste liquid in the waste liquid buffer barrel.

[0015] Optionally, the special cleaning fluid module includes: a sixth pump body and a special cleaning fluid barrel, the special cleaning fluid barrel is connected to the sample cleaning pool through the sixth pump body; a seventh liquid level sensor is provided in the special cleaning fluid barrel, the sixth pump body and the seventh liquid level sensor are connected to the controller, and the seventh liquid level sensor is used to monitor the liquid level in the special cleaning fluid barrel.

[0016] Optionally, the excitation liquid module includes an excitation liquid barrel and at least one metering pump, and the excitation liquid barrel is connected to the metering pump group.

[0017] Optionally, the excitation liquid module also includes an excitation liquid spare barrel and a third solenoid valve group. The excitation liquid spare barrel and the excitation liquid barrel are connected to the input end of the third solenoid valve group, the output end of the third solenoid valve group is connected to the metering pump group, and the controller is electrically connected to the third solenoid valve group and the metering pump group. When the excitation liquid capacity in the excitation liquid barrel is lower than the preset capacity threshold, the controller controls the third solenoid valve group to enable the excitation liquid spare barrel to supply liquid to the metering pump group.

[0018] An embodiment of the present application also proposes a chemiluminescence immunoassay analyzer, comprising a cleaning tray, a detection device, a sample needle robotic arm, a reagent needle robotic arm, and also comprising a liquid circuit system as described in any of the foregoing items, wherein the first cleaning module in the liquid circuit system provides system liquid or special cleaning liquid to the sample needle robotic arm and the reagent needle robotic arm respectively, the second cleaning module in the liquid circuit system provides cleaning liquid to the cleaning tray respectively, and the excitation liquid module provides excitation liquid to the detection device respectively; the waste liquid generated by the cleaning tray, the detection device, the sample needle robotic arm and the reagent needle robotic arm is discharged through the waste liquid module.

[0019] The embodiments proposed in this application can provide a complete fluid circuit system for the use of a chemiluminescence immunoassay instrument. The fluid circuit system proposed in this application can meet the use requirements of each functional unit in the chemiluminescence immunoassay instrument. In addition, the fluid circuit system can perform multiple actions simultaneously, saving time. The operation of the fluid circuit system in each functional unit is not easily affected by other functional units. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Below, the preferred embodiments of the present invention will be further described in detail with reference to the accompanying drawings, in which:

[0021] Figure 1 Schematic diagram of a liquid circuit system of a chemiluminescent immunoassay analyzer provided in accordance with an embodiment of the present application;

[0022] Figure 2 Schematic diagram of a fluid path system of another chemiluminescent immunoassay analyzer provided in accordance with an embodiment of the present application;

[0023] Figure 3 1 is a schematic diagram of a system liquid branch of a liquid system of a chemiluminescence immunoassay analyzer provided in an embodiment of the present application;

[0024] Figure 4 Schematic diagram of a liquid circuit system of another chemiluminescence immunoassay analyzer provided in an embodiment of the present application;

[0025] Figure 5 This is a schematic diagram of a cleaning liquid branch in a liquid system of a chemiluminescence immunoassay analyzer provided in an embodiment of the present application;

[0026] Figure 6 This is a schematic diagram of a waste liquid module in a liquid path system of a chemiluminescence immunoassay analyzer provided in an embodiment of the present application;

[0027] Figure 7 This is a schematic diagram of a waste liquid module in a liquid path system of another chemiluminescence immunoassay instrument provided in an embodiment of the present application;

[0028] Figure 8 This is a schematic diagram of the overall structure of a chemiluminescence immunoassay provided according to an embodiment of the present application. DETAILED DESCRIPTION

[0029] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0030] In the detailed description that follows, reference may be made to the various drawings that form part of this application and illustrate specific embodiments of the present application. In the drawings, similar reference numerals describe substantially similar components in different figures. Each specific embodiment of the present application is described below in sufficient detail to enable a person of ordinary skill in the art to implement the technical solutions of the present application. It should be understood that other embodiments may be utilized or that structural, logical, or electrical changes may be made to the embodiments of the present application.

[0031] Figure 1 Schematic diagram of the liquid path system of a chemiluminescent immunoassay analyzer provided in accordance with an embodiment of the present application. Figure 1 The liquid path system 100 of the chemiluminescence immunoassay analyzer includes: a liquid replenishing module 101, a first cleaning module 102, a second cleaning module 103, a waste liquid module 104 and an excitation liquid module 105, wherein the liquid replenishing module 101 includes a system liquid branch 1011 and a cleaning liquid branch 1012.

[0032] The system liquid branch 1011 is connected to the first cleaning module 102, and the system liquid branch 1011 is controlled by the controller 106 to provide system liquid for cleaning the sample needle and the reagent needle. In the chemiluminescent immunoassay, the sample needle is arranged on the robotic arm and is used to extract the sample into the reaction tube. Since each sample has differences, in order to ensure the accuracy of the test, the sample needle after the sample is extracted needs to be cleaned. The system liquid is a liquid with the ability to clean the sample needle. Similarly, the reagent needle is a needle used to extract the reagent into the reaction tube in the chemiluminescent immunoassay. It needs to be cleaned in time after use. The system liquid can be used for cleaning the reagent needle. The system liquid branch 1011 is controlled by the controller 106 to achieve cleaning of the sample needle and the reagent needle.

[0033] The cleaning liquid branch 1012 is connected to the second cleaning module 103. The controller 106 controls the cleaning liquid branch 1012 to supply cleaning liquid to the dosing needle, which is used to clean the reaction tubes. The second cleaning module is connected to the dosing needle located in the cleaning tray. The dosing needle needs to supply cleaning liquid to the reaction tubes located in the cleaning tray. The cleaning liquid is a liquid configured to remove substances in the reaction tubes that are not bound to the magnetic beads. The cleaning liquid branch 1012 is connected to the cleaning module 103 and can provide cleaning liquid for cleaning the dosing needle.

[0034] The waste liquid module 104 is connected to the first cleaning module 102 and the second cleaning module 103, respectively. The controller 106 controls the waste liquid module 104 to receive waste liquid generated by the first cleaning module 102, the second cleaning module 103, and the reagent reservoir 345. The excitation liquid module 105 is controlled by the controller 106 and is used to provide excitation liquid for the chemiimmunoluminometer. The excitation liquid is a liquid used to excite the luminescent substance.

[0035] The fluidic system proposed in this application is divided into multiple functional modules, each of which is used to provide the required liquid to the corresponding functional unit in the chemiluminescent immunoassay, thereby meeting the usage requirements of each functional unit in the chemiluminescent immunoassay. In addition, each module of the fluidic system can perform multiple actions simultaneously without being affected by the operation of other fluidic systems in each functional unit.

[0036] Figure 2 FIG. 1 is a schematic diagram of a liquid circuit system of another chemiluminescent immunoassay analyzer provided in an embodiment of the present application. Figure 2As shown, the liquid circuit system 200 also includes a special cleaning liquid module 201, and the special cleaning liquid module 201 is connected to the sample needle in the first cleaning module 102, and the controller 106 controls the special cleaning liquid module 201 to provide special cleaning liquid for cleaning the sample needle. In some embodiments, optionally, the special cleaning liquid module 201 can also be connected to the reagent needle to provide a special cleaning liquid for cleaning the reagent needle. In a chemiluminescent immunoassay, the sample needle and / or reagent needle will come into contact with special samples or reagents during the detection process. For example, when the sample is infectious or the reagent is highly corrosive, the sample needle and reagent needle cannot be cleaned using system fluid or cleaning fluid. Therefore, the liquid circuit system of the chemiluminescent immunoassay provided in the embodiment of the present application can provide a special cleaning liquid for cleaning the sample needle and / or reagent needle to improve the accuracy of the detection.

[0037] Figure 3 Schematic diagram of the system liquid branch of the liquid system of a chemiluminescent immunoassay instrument provided in an embodiment of the present application. Figure 3 As shown, the system fluid branch 1011 includes a system fluid reservoir 308, within which is located a first liquid level sensor 302 for monitoring the liquid level within the system fluid reservoir 301. In some embodiments, the system fluid branch 1011 optionally further includes a liquid filter 303 and a Luer connector 306. The liquid filter 303 is used to remove impurities from the system fluid, allowing subsequent branches to provide relatively pure system fluid and eliminating the adverse effects of impurities. The Luer connector 306 is connected to the fluid path from the output of the system fluid reservoir 308 to the filter 303. This eliminates the need to replace other fluid paths when switching to other types of system fluid, preventing contamination and facilitating the change of system fluid types. Furthermore, the first liquid level sensor 302 can be electrically connected to the controller 106. When the liquid level within the system fluid reservoir falls below a preset value, the first liquid level sensor 302 transmits a liquid level signal to the controller 106. Upon receiving the signal, the controller 106 can issue an alarm or prompt, allowing staff to replenish the system fluid promptly.

[0038] Figure 4 FIG. 1 is a schematic diagram of a liquid path system of another chemiluminescence immunoassay analyzer provided in an embodiment of the present application. Figure 4As shown, the system liquid branch circuit 1011 also includes a system liquid buffer tank 301, which is connected to a system liquid storage tank 308 via a first pump body 304. A second liquid level sensor 307 is located within the system liquid buffer tank. The controller 106 is electrically connected to the first pump body 304 and the second liquid level sensor 307. The second liquid level sensor includes a second high-level float and a second low-level float. The second high-level float is used for fluid refill monitoring, while the second low-level float is used for alarm monitoring. When the second high-level float detects that the liquid level within the system liquid buffer tank 301 is below a second high-level preset value, the controller 106 controls the first pump body 304 to pump system liquid from the system liquid storage tank 308 into the system liquid buffer tank 301. When the second low-level float detects that the liquid level within the system liquid buffer tank is below a second low-level preset value, the controller controls an alarm to sound an alarm. Both the second high-level float and the second low-level float are sensors that sense the liquid level within the system liquid buffer tank. The first pump body 304 can be a diaphragm pump, a plunger pump, a centrifugal pump, etc., as long as it can pump the system liquid from the system liquid storage tank 308 into the system liquid buffer tank 301. In some embodiments, the first pump body 304 is preferably a diaphragm pump.

[0039] In the system liquid branch 1011, the system liquid buffer barrel 301 first provides system liquid to the first cleaning module. When the liquid level in the system liquid buffer barrel is lower than the second highest liquid level preset value, the controller 106 turns on the first pump body 304, and the first pump body 304 draws the system liquid in the system liquid storage barrel into the system liquid buffer barrel. In this way, the chemiluminescence immunoassay analyzer can obtain continuous replenishment of system liquid in a timely manner without stopping work. Compared with the system liquid branch 1101 without the system liquid buffer barrel, the provision of the system liquid buffer barrel 301 improves the working efficiency of the chemiluminescence immunoassay analyzer, and the system liquid replenishment work can be completed without shutting down the analyzer.

[0040] Figure 5 FIG. 1 is a schematic diagram of a cleaning liquid branch in a liquid system of a chemiluminescent immunoassay analyzer according to an embodiment of the present application. Figure 5As shown, the cleaning fluid branch circuit 1012 includes a cleaning fluid reservoir 318, within which is located a third liquid level sensor 312 for monitoring the liquid level within the cleaning fluid reservoir. In some embodiments, the cleaning fluid branch circuit 1012 optionally also includes a liquid filter 303 and a Luer connector 306. The liquid filter 303 is used to remove impurities from the system fluid, allowing subsequent branches to provide relatively pure system fluid and eliminating the adverse effects of impurities. The Luer connector 306 is connected to the fluid path from the output of the cleaning fluid reservoir 318 to the liquid filter 303. This eliminates the need to replace other fluid paths when switching to other types of system fluid, preventing contamination and facilitating the change of cleaning fluid types. Furthermore, the third liquid level sensor 312 can be electrically connected to the controller 106. When the liquid level within the cleaning fluid reservoir falls below a preset value, the third liquid level sensor 312 transmits a liquid level signal to the controller 106. Upon receiving the signal, the controller 106 issues an alarm or prompts staff to replenish the cleaning fluid promptly.

[0041] Continue to see Figure 4 As shown, in some embodiments, the cleaning fluid branch circuit 1012 further includes a cleaning fluid buffer tank 315, which is connected to a cleaning fluid storage tank 318 via a second pump body 314. A fourth liquid level sensor 313 is located within the cleaning fluid buffer tank 315. A controller is electrically connected to the second pump body 314 and the fourth liquid level sensor 313. The fourth liquid level sensor includes a fourth high-level float and a fourth low-level float. The fourth high-level float is used for fluid refill monitoring, while the fourth low-level float is used for alarm monitoring. When the fourth high-level float detects that the liquid level within the cleaning fluid buffer tank 315 is below a predetermined fourth high-level value, the controller controls the second pump body 314 to pump cleaning fluid from the cleaning fluid storage tank 318 into the cleaning fluid buffer tank 315. When the fourth low-level float detects that the liquid level within the cleaning fluid buffer tank is below a predetermined fourth low-level value, the controller controls an alarm to sound an alarm. In some embodiments, both the second high-level float and the second low-level float can optionally serve as sensors that can sense the liquid level within the system liquid buffer tank. The second pump body 314 can be a diaphragm pump, a plunger pump, a centrifugal pump, etc., as long as it can pump the system liquid from the cleaning liquid storage tank 318 into the system liquid buffer tank 313. In some embodiments, the second pump body 314 is preferably a diaphragm pump.

[0042] In the system liquid branch 1011, the cleaning liquid buffer barrel 313 is used to ensure that the chemiluminescence immunoassay analyzer can replenish the cleaning liquid in a timely manner without stopping the work. Compared with the cleaning liquid branch 1012 without the cleaning liquid buffer barrel, the provision of the cleaning liquid buffer barrel improves the working efficiency of the chemiluminescence immunoassay analyzer, and the cleaning liquid replenishment can be completed without shutting down the analyzer.

[0043] like Figure 4As shown, the first cleaning module 102 includes an external cleaning branch and an internal cleaning branch. The external cleaning branch includes at least one first diaphragm pump, the second solenoid valve 3242 and the fourth solenoid valve 3244 in the first solenoid valve group, and a liquid supply pipeline with a first end liquid outlet connected to the sample cleaning tank 323 or the reagent cleaning tank 322. The internal cleaning branch includes at least one second diaphragm pump, the first solenoid valve 3241 and the third solenoid valve 3243 in the first solenoid valve group, at least one first plunger pump 3251, and a liquid supply pipeline with liquid outlets 329 and 328 connected to the interior of the sample needle or reagent needle. The controller 106 controls the liquid supply to the external cleaning branch and the internal cleaning branch separately or simultaneously.

[0044] It needs to be explained that, Figure 4As shown, the first solenoid valve group 324 includes a first solenoid valve 3241, a second solenoid valve 3242, a third solenoid valve 3243, and a fourth solenoid valve 3244. A three-way connector 321 divides the fluid path into two branches, each connected to the first solenoid valve group 324 via a pump body 3261 and a pump body 3262. Pump body 3261 is a first diaphragm pump, and pump body 3262 is a second diaphragm pump. Alternatively, pump body 3261 is a first diaphragm pump, and pump body 3262 is a second diaphragm pump. The first solenoid valve group 324 is electrically connected to the controller 106, which can control the order in which the solenoid valves in the first solenoid valve group 324 are used. The output of the first solenoid valve 3241 in the first solenoid valve group 324 is connected to the input of the first plunger pump 3251, and the output of the first plunger pump 3251 is connected to the sample injection needle via a needle blockage detection element 327. The output end of the second solenoid valve 3242 is connected to the liquid circuit terminal through a liquid pipeline and is connected to the base of the sample injection needle through a three-way connector 321. The output end of the third solenoid valve 3243 is connected to the input end of the first plunger pump 3251, and the output end of the first plunger pump 3251 is connected to the reagent needle through a liquid pipeline. The output end of the fourth solenoid valve 3244 is connected to the liquid circuit terminal 329 through a liquid pipeline and is further connected to the base of the reagent needle through a three-way connector. When it is necessary to clean the inner wall of the sampling needle, the controller 106 controls the first solenoid valve 3241 to be turned on, the first plunger pump 3251 to work, and the system fluid flows through the first solenoid valve 3241 through the pump body 3261 and / or the pump body 3262, and rushes into the inner wall of the sampling needle under the greater pressure of the first plunger pump 3251 to clean the inner wall of the sampling needle; when it is necessary to clean the outer wall of the sampling needle, the controller 106 controls the second solenoid valve 3242 to be turned on, the pump body 3261 and / or the pump body 3262 draws the system fluid to flow through the third solenoid valve 3243, and then the end of the outflow liquid path is connected to the sample cleaning pool 323 through the three-way connector 321 to clean the outer wall of the sampling needle. The same workflow is used for cleaning the reagent needle. When the inner wall of the reagent needle needs to be cleaned, the controller 106 controls the fifth solenoid valve 3243 to be turned on, and the first plunger pump 3251 starts working. The system fluid flows through the third solenoid valve 3243 through the pump body 3261 and / or the pump body 3262, and under the action of the higher pressure of the first plunger pump 3251, it rushes into the inner wall of the reagent needle to clean the inner wall of the reagent needle. When the outer wall of the reagent needle needs to be cleaned, the controller 106 controls the fourth solenoid valve 3244 to be turned on, and the pump body 3261 and / or the pump body 3262 draws the system fluid to flow through the fourth solenoid valve 3244. The end of the outflow liquid path is connected to the reagent cleaning tank 322 through the three-way connector 321 to clean the outer wall of the reagent needle. The first plunger pump 3251 is connected to the controller 106, and the controller controls the opening and closing of the first plunger pump. When the first solenoid valve 3241 is closed, the controller controls the first plunger pump 3251 to provide power for the sample needle to absorb the sample.Likewise, when the third solenoid valve 3243 is closed, the controller controls the first plunger pump 3251 to provide power for the reagent needle to draw the reagent.

[0045] The controller 106 opens the corresponding solenoid valves based on the operating requirements of the sample and reagent needles in the chemiluminescence immunoassay, controls the opening and closing of the solenoid valves in the first solenoid valve group 324, and thereby controls the cleaning sequence of the inner and outer walls of the sample and reagent needles. The first solenoid valve 3241, the second solenoid valve 3242, the third solenoid valve 3243, and the fourth solenoid valve 3244 can all be two-way solenoid valves.

[0046] By setting up multiple cleaning branches, the cleaning of the sample needle and the reagent needle are not affected by each other, and multiple tasks can be performed simultaneously, thereby improving the working efficiency and stability of the chemiluminescence immunoassay.

[0047] When special cleaning of the sample injection needle or reagent needle is required, the special cleaning fluid module 201 is equipped with a special cleaning fluid barrel 352. A seventh liquid level sensor 353 is installed inside the special cleaning fluid barrel 352. The seventh liquid level sensor 353 is used to detect the liquid level in the special cleaning fluid barrel. When the liquid level in the special cleaning fluid barrel falls below a preset value of the special cleaning fluid, the controller controls the alarm to sound an alarm signal, and the staff will add special cleaning fluid to the special cleaning fluid barrel. The special cleaning fluid barrel 352 is connected to the sample injection cleaning tank via the Luer connector 306, the first reducer 355, the first through-plate connector 354, the diaphragm pump body 356, the sixth pump body 356, the second through-plate connector 357, and the second reducer 351. Similarly, the end of the liquid path of the special cleaning fluid module can also be connected to the reagent cleaning tank.

[0048] Continue to see Figure 4The second cleaning module 103 includes multiple cleaning branches connected to a dosing needle at their ends. Each of the cleaning branches includes at least one second plunger pump 325 and corresponding fifth and sixth solenoid valves 334 and 333. A controller controls the multiple cleaning branches to supply cleaning fluid in a predetermined sequence. Specifically, the second cleaning module 103 includes at least one second plunger pump 325 and a second solenoid valve group 332. The second solenoid valve group 332 includes a fifth solenoid valve 333 and multiple sixth solenoid valves 334. In some embodiments, the fifth and sixth solenoid valves 333 and 334 may be two-way solenoid valves. The input of the fifth solenoid valve 333 is connected to the output of the cleaning fluid branch, and the output of the fifth solenoid valve 333 is connected to the input of the second plunger pump 325. The output of the second plunger pump 325 is connected to the input of multiple sixth solenoid valves 334. The output of the sixth solenoid valve 334 is connected to the dosing needle. In some embodiments, there are three sixth solenoid valves 334, each connected to the dosing needle via a fluid line. The controller 106 is connected to the second solenoid valve group 332 and is used to control the switching sequence of each solenoid valve in the second solenoid valve group. The second plunger pump 325 is connected to the controller and is used to provide power for the liquid addition needle to clean the reaction tube.

[0049] All the solenoid valves in the second solenoid valve group 332 are turned on, and the cleaning liquid drawn by the second plunger pump 325 flows through the fifth solenoid valve 333, then enters the second plunger pump and is transported to the sixth solenoid valve 334 and then flows into the liquid adding needle 337, which outputs the cleaning liquid into the reaction tube.

[0050] Figure 6 Schematic diagram of a waste liquid module in a liquid path system of a chemiluminescent immunoassay according to an embodiment of the present application. Figure 6 As shown, the waste liquid module includes a first cleaning module waste liquid branch, a second cleaning module waste liquid branch, a reagent tank waste liquid branch, and a waste liquid barrel 348. The first cleaning module waste liquid branch includes at least one third pump body 343, which is connected to a controller. The controller controls the third pump body 343 to pump the waste liquid generated by the first cleaning module 102 into the waste liquid barrel 348. The second cleaning module waste liquid branch includes a peristaltic pump 342, the input end of the peristaltic pump 342 is connected to a pipette needle 341, and the peristaltic pump 342 is connected to the controller. The controller controls the peristaltic pump 342 to pump the waste liquid generated by the second cleaning module 103 into the waste liquid barrel 348. The outlet of the reagent tank branch is connected to the waste liquid barrel 348, and the waste liquid in the reagent tank 345 is discharged into the waste liquid barrel. A fifth liquid level sensor 349 is provided in the waste liquid barrel 348, and the fifth liquid level sensor 349 is used to monitor the liquid level in the waste liquid barrel. When the fifth liquid level sensor 349 monitors that the liquid level in the waste liquid barrel is higher than its preset liquid level value, the controller sends a signal and the staff pours out the waste liquid in the waste liquid barrel 348.

[0051] like Figure 4 As shown, in some embodiments, optionally, the waste liquid module includes a first cleaning module waste liquid branch, a second cleaning module waste liquid branch, a reagent tank waste liquid branch, a waste liquid barrel 348 and a waste liquid buffer barrel 346, wherein the first cleaning module waste liquid branch includes at least one third pump body 343, the third pump body 343 is connected to the controller 106, and the controller controls the third pump body to extract the waste liquid generated by the first cleaning module into the waste liquid buffer barrel 346; the second cleaning module waste liquid branch includes a peristaltic pump 342, the input end of the peristaltic pump 342 is connected to the aspiration needle 341, the peristaltic pump is connected to the controller, and the controller controls the peristaltic pump to extract the waste liquid generated by the second cleaning module into the waste liquid buffer barrel; the outlet of the reagent tank branch is connected to the waste liquid buffer barrel, and the waste liquid generated by the reagent tank 345 is discharged to the waste liquid buffer barrel 346.

[0052] A sixth liquid level sensor 347 is installed in the waste liquid buffer barrel 346 and is connected to the controller 106 to monitor the liquid level in the waste liquid buffer barrel 346. When the sixth liquid level sensor detects that the liquid level is below a preset value, an alarm is issued. A fifth liquid level sensor 349 is installed in the waste liquid barrel 348. The waste liquid barrel 348 is connected to the waste liquid buffer barrel 346 via the fourth pump body 3441 and the Luer connector 306. The fifth liquid level sensor and the fourth pump body are connected to the controller, and the fifth liquid level sensor is used to detect the liquid level in the waste liquid barrel. When the fifth liquid level sensor detects that the liquid level is above the preset value for the waste liquid, the controller issues an alarm, prompting staff to discard the waste liquid in the waste liquid barrel.

[0053] The waste liquid flowing out of the first cleaning module waste liquid branch and the second cleaning module waste liquid branch can first flow into the waste liquid buffer barrel and then flow into the waste liquid barrel, thereby enabling the chemiluminescence immunoassay analyzer to continuously discharge the waste liquid without stopping its operation.

[0054] Figure 7 Schematic diagram of a waste liquid module in the liquid path system of another chemiluminescent immunoassay provided in accordance with an embodiment of the present application. The waste liquid module includes a first cleaning module waste liquid branch, a second cleaning module waste liquid branch, a reagent tank waste liquid branch, a waste liquid buffer barrel 346, and a sewer discharge branch, wherein the first cleaning module waste liquid branch includes at least one third pump body 343, the third pump body 343 is connected to a controller, and the controller controls the third pump body to pump the waste liquid generated by the first cleaning module into the waste liquid buffer barrel 346; the second cleaning module waste liquid branch includes a peristaltic pump 342, the input end of the peristaltic pump 342 is connected to a pipette needle 341, the peristaltic pump is connected to the controller, and the controller controls the peristaltic pump to pump the waste liquid generated by the second cleaning module into the waste liquid buffer barrel; the outlet of the reagent tank branch is connected to the waste liquid buffer barrel, and the waste liquid generated by the reagent tank 345 is discharged into the waste liquid buffer barrel 346.

[0055] A sixth liquid level sensor 347 is provided within the waste liquid buffer barrel 346. This sixth liquid level sensor 347 is connected to the controller and is used to monitor the liquid level within the waste liquid buffer barrel 346. When the sixth liquid level sensor detects a liquid level below a preset value, an alarm is sounded. The sewer discharge branch is connected to the waste liquid buffer barrel 346 via the fifth pump body 3442 and is used to discharge the waste liquid within the waste liquid buffer barrel. The waste liquid module also includes a sewer discharge branch. The sewer discharge branch input end is connected to the waste liquid buffer barrel 346 and is discharged to the sewer discharge port 340 through the through-plate joint 354. The sewer discharge branch is used to discharge waste liquid from the waste liquid buffer barrel.

[0056] The excitation liquid module 105 includes an excitation liquid barrel 361 and at least one metering pump 362, and the excitation liquid barrel 361 is connected to the at least one metering pump 362. In some embodiments, optionally, the excitation liquid module 105 also includes an excitation liquid standby barrel 364 and a third solenoid valve group 363, the excitation liquid standby barrel 364 and the excitation liquid barrel 361 are connected to the input end of the third solenoid valve group 363, the output end of the third solenoid valve group 363 is connected to the at least one metering pump 362, the controller is electrically connected to the third solenoid valve group 363 and the at least one metering pump 362, and when the excitation liquid capacity in the excitation liquid barrel 361 is lower than the preset capacity threshold, the controller controls the third solenoid valve group 363 to enable the excitation liquid standby barrel 364 to supply liquid to the at least one metering pump 362. Figure 4 As shown, solenoid valve assembly 363 is connected to metering pump 362, solenoid valve 3631 is connected to stimulating liquid barrel 361, and solenoid valve 3632 is connected to stimulating liquid reserve barrel 364. When the liquid volume in stimulating liquid barrel 361 falls below a preset volume threshold, solenoid valve 3631 closes, solenoid valve 3632 opens, and metering pump draws stimulating liquid from reserve barrel 364. At this point, staff can replenish stimulating liquid in stimulating liquid barrel 361, ensuring sufficient stimulating liquid is provided while the chemiluminescence immunoassay analyzer continues to operate.

[0057] Figure 8 This is a schematic diagram of the overall structure of a chemiluminescence immunoassay analyzer provided in accordance with an embodiment of the present application. Figure 7 As shown, the present application also proposes a chemiluminescence immunoassay instrument, comprising a cleaning tray 702, a detection device 701, a sample needle robot 704, a reagent needle robot 706, an incubation tray 703, a reagent chamber 707 and a tube library 705, and also comprising a liquid circuit system as described in any of the foregoing items, wherein the first cleaning module in the liquid circuit system provides system liquid or special cleaning liquid corresponding to the sample needle robot and the reagent needle robot, the second cleaning module in the liquid circuit system provides cleaning liquid corresponding to the cleaning tray, and the excitation liquid module provides excitation liquid corresponding to the detection device; the waste liquid generated by the cleaning tray, the detection device, the sample needle robot and the reagent needle robot is discharged through the waste liquid module.

[0058] The chemiluminescent immunoassay analyzer proposed in the embodiments of the present application can meet the cleaning, detection, sample and reagent probe robotic arms' requirements for cleaning, system and excitation fluids, as well as waste fluid disposal. In the chemiluminescent immunoassay analyzer proposed in the embodiments of the present application, the fluid circuit systems of the various unit modules can operate simultaneously without interfering with each other, which can also save time and improve the operating efficiency of the chemiluminescent immunoassay analyzer to a certain extent.

[0059] The above embodiments are only used to illustrate the present invention, and are not intended to limit the present invention. Ordinary technicians in the relevant technical field can make various changes and modifications without departing from the scope of the present invention. Therefore, all equivalent technical solutions should also fall within the scope of the present invention.

Claims

1. A liquid path system of a chemiluminescent immunoassay analyzer, characterized in that: include: A liquid replenishing module, a first cleaning module, a second cleaning module, a waste liquid module and an excitation liquid module, wherein: The fluid replenishment module includes a system fluid branch and a cleaning fluid branch, the system fluid branch includes: a system fluid storage tank, the cleaning fluid branch includes: a cleaning fluid storage tank; The system liquid branch is connected to the first cleaning module, and the controller controls the system liquid branch to provide system liquid from the system liquid storage barrel for cleaning the sample needle and the reagent needle; The cleaning liquid branch is connected to the second cleaning module, and the controller controls the cleaning liquid branch to provide the liquid adding needle with cleaning liquid from the cleaning liquid storage barrel, and the cleaning liquid is used to clean the reagents in the reaction tube; The waste liquid module is connected to the first cleaning module, the second cleaning module and the reagent tank respectively, and the controller controls the waste liquid module to receive waste liquid generated by the first cleaning module, the second cleaning module and the reagent tank; The excitation liquid module, the controller controls the excitation liquid module to provide excitation liquid for the chemiimmunoluminescence instrument, for exciting the luminescent substance; The liquid circuit system also includes: a special cleaning fluid module; The end of the system liquid branch is divided into two branches through a three-way joint, and the two branches are respectively connected to the first solenoid valve group through the first diaphragm pump and the second diaphragm pump. The first solenoid valve group includes a first solenoid valve, a second solenoid valve, a third solenoid valve and a fourth solenoid valve. The first solenoid valve group is electrically connected to the controller, and the controller controls the use order of each solenoid valve in the first solenoid valve group. The output end of the first solenoid valve in the first solenoid valve group is connected to the input end of the first plunger pump, and the output end of the first plunger pump is connected to the sample addition needle through a needle blockage detection element. The output end of the second solenoid valve is connected to the end of the liquid circuit through a liquid pipeline and is connected to the sample cleaning pool through a three-way joint. The output end of the third solenoid valve is connected to the input end of the first plunger pump, and the output end of the first plunger pump is connected to the reagent needle through a liquid pipeline. The output end of the fourth solenoid valve is connected to the end of the liquid circuit through a liquid pipeline, and then connected to the reagent cleaning pool through a three-way joint.

2. The liquid path system of the chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The special cleaning fluid module is connected to the first cleaning module, and the controller controls the special cleaning fluid module to provide special cleaning fluid for cleaning the sample needle and / or the reagent needle.

3. The liquid path system of the chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The system liquid branch includes: a system liquid storage barrel, a first liquid level sensor is provided in the system liquid storage barrel, the first liquid level sensor is connected to the controller, the first liquid level sensor is used to monitor the liquid level in the system liquid storage barrel, and output a liquid level monitoring signal to the controller.

4. The liquid path system of the chemiluminescence immunoassay analyzer according to claim 3, characterized in that: The system liquid branch also includes: a system liquid buffer barrel, which is connected to the system liquid storage barrel through a first pump body. A second liquid level sensor is provided in the system liquid storage barrel. The controller is electrically connected to the first pump body and the second liquid level sensor. The second liquid level sensor includes a second high-level float and a second low-level float. The second high-level float is used for fluid replenishment monitoring, and the second low-level float is used for alarm monitoring.

5. The liquid path system of the chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The cleaning liquid branch includes: a cleaning liquid storage barrel, a third liquid level sensor is provided in the cleaning liquid storage barrel, the third liquid level sensor is connected to the controller, and the third liquid level sensor is used to monitor the liquid level in the cleaning liquid storage barrel and output a liquid level monitoring signal to the controller.

6. The liquid path system of the chemiluminescence immunoassay analyzer according to claim 5, characterized in that: The cleaning liquid branch also includes: a cleaning liquid buffer barrel, which is connected to the cleaning liquid storage barrel through a second pump body. A fourth liquid level sensor is provided in the cleaning liquid buffer barrel. The controller is electrically connected to the second pump body and the fourth liquid level sensor. The fourth liquid level sensor includes a fourth high-level float and a fourth low-level float. The fourth high-level float is used for liquid replenishment monitoring, and the fourth low-level float is used for alarm monitoring.

7. The liquid path system of the chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The second cleaning module includes: multiple cleaning branches whose ends are connected to the liquid adding needles, each of the cleaning branches includes at least one second plunger pump and a corresponding solenoid valve, and the controller controls the multiple cleaning branches to supply liquid for cleaning in a predetermined order.

8. The liquid path system of the chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The waste liquid module includes a first cleaning module waste liquid branch, a second cleaning module waste liquid branch, a reagent tank waste liquid branch and a waste liquid barrel, wherein: The waste liquid branch of the first cleaning module includes at least one third pump body, the third pump body is connected to the controller, and the controller controls the third pump body to pump the waste liquid generated by the first cleaning module into the waste liquid bucket; The waste liquid branch of the second cleaning module includes a peristaltic pump, the input end of the peristaltic pump is connected to the liquid suction needle, the peristaltic pump is connected to the controller, and the controller controls the peristaltic pump to extract the waste liquid generated by the second cleaning module into the waste liquid bucket; The waste liquid branch outlet of the reagent tank is connected to the waste liquid barrel; A fifth liquid level sensor is provided in the waste liquid barrel, and the fifth liquid level sensor is used to monitor the liquid level in the waste liquid barrel and output a liquid level monitoring signal to the controller.

9. The liquid path system of the chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The waste liquid module includes a first cleaning module waste liquid branch, a second cleaning module waste liquid branch, a reagent tank waste liquid branch, a waste liquid barrel and a waste liquid buffer barrel, wherein: The waste liquid branch of the first cleaning module includes at least one third pump body, the third pump body is connected to the controller, and the controller controls the third pump body to pump the waste liquid generated by the first cleaning module into the waste liquid buffer tank; The waste liquid branch of the second cleaning module includes a peristaltic pump, the input end of the peristaltic pump is connected to the liquid suction needle, the peristaltic pump is connected to the controller, and the controller controls the peristaltic pump to extract the waste liquid generated by the second cleaning module into the waste liquid buffer barrel; The waste liquid branch outlet of the reagent tank is connected to the waste liquid buffer barrel; A sixth liquid level sensor is provided in the waste liquid buffer barrel, and the sixth liquid level sensor is connected to the controller and is used to monitor the liquid level in the waste liquid buffer barrel; A fifth liquid level sensor is provided in the waste liquid barrel, the waste liquid barrel is connected to the waste liquid buffer barrel through a fourth pump body, the fifth liquid level sensor and the fourth pump body are connected to the controller, and the fifth liquid level sensor is used to monitor the liquid level in the waste liquid barrel.

10. The liquid path system of the chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The waste liquid module includes a first cleaning module waste liquid branch, a second cleaning module waste liquid branch, a reagent tank waste liquid branch, a waste liquid buffer tank and a sewer discharge branch, wherein: The waste liquid branch of the first cleaning module includes at least one third pump body, and the at least one third pump body is connected to the controller. The controller controls the at least one third pump body to pump the waste liquid generated by the first cleaning module into the waste liquid buffer tank; The waste liquid branch of the second cleaning module includes a peristaltic pump, the input end of the peristaltic pump is connected to the liquid suction needle, the peristaltic pump is connected to the controller, and the controller controls the peristaltic pump to extract the waste liquid generated by the second cleaning module into the waste liquid buffer barrel; The reagent tank waste liquid branch is connected to the waste liquid buffer barrel; A sixth liquid level sensor is provided in the waste liquid buffer barrel, and the sixth liquid level sensor is used to monitor the liquid level in the waste liquid buffer barrel and output a liquid level monitoring signal to the controller; The sewer discharge branch is connected to the waste liquid buffer barrel through the fifth pump body, and is used for discharging the waste liquid in the waste liquid buffer barrel.

11. The liquid path system of the chemiluminescence immunoassay analyzer according to claim 2, characterized in that: The special cleaning liquid module includes: a sixth pump body and a special cleaning liquid barrel, and the special cleaning liquid barrel is connected to the sample cleaning pool through the sixth pump body; A seventh liquid level sensor is provided in the special cleaning liquid barrel, the sixth pump body and the seventh liquid level sensor are connected to the controller, and the seventh liquid level sensor is used to monitor the liquid level in the special cleaning liquid barrel.

12. The liquid path system of the chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The excitation liquid module includes an excitation liquid barrel and at least one metering pump, and the excitation liquid barrel is connected to the metering pump group.

13. The liquid path system of the chemiluminescence immunoassay analyzer according to claim 12, characterized in that: The excitation liquid module also includes an excitation liquid spare barrel and a third solenoid valve group. The excitation liquid spare barrel and the excitation liquid barrel are connected to the input end of the third solenoid valve group, and the output end of the third solenoid valve group is connected to the metering pump group. The controller is electrically connected to the third solenoid valve group and the metering pump group. When the excitation liquid capacity in the excitation liquid barrel is lower than the preset capacity threshold, the controller controls the third solenoid valve group to enable the excitation liquid spare barrel to supply liquid to the metering pump group.

14. A chemiluminescence immunoassay analyzer comprising a cleaning tray, a detection device, a sample needle robotic arm, and a reagent needle robotic arm, characterized in that: It also includes a liquid circuit system as described in any one of claims 1 to 13, wherein the first cleaning module in the liquid circuit system provides the system liquid or special cleaning liquid to the sample needle robot arm and the reagent needle robot arm respectively, the second cleaning module in the liquid circuit system provides cleaning liquid to the cleaning tray respectively, and the excitation liquid module provides excitation liquid to the detection device respectively; the waste liquid generated by the cleaning tray, the detection device, the sample needle robot arm and the reagent needle robot arm is discharged through the waste liquid module.

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