Preparation methods and biological applications of cerium phytate complexes

Abstract

This invention discloses a method for preparing cerium phytate complexes and their biological applications. A cerium ion aqueous solution is added to a phytic acid aqueous solution under stirring, and stirring continues until precipitation is complete. The mixture is then washed with ultrapure water until the washing solution is neutral, filtered, and dried to obtain a cerium phytate complex with a porous nanostructure. This invention utilizes a method for preparing cerium phytate complexes with the above-described structure and their biological applications, without using organic solvents, making the synthesis process safe and environmentally friendly. Phytic acid, as a ligand molecule constituting cerium phytate, has six phosphate groups, which can efficiently coordinate with cerium ions to form cerium phytate materials, exhibiting good thermal stability and ultraviolet absorption. The cerium phytate nanoparticles formed by the coordination of phytic acid and cerium ions carry a large amount of Ce. 3+ This is the basis for the antioxidant activity of cerium phytate. At the same time, the numerous pores inside the material effectively promote the adsorption of reactive oxygen free radicals, giving it highly efficient antioxidant properties, which can be applied to the treatment of various diseases related to reactive oxygen species.

Description

Technical Field

[0001] This invention relates to the field of antioxidant technology, and in particular to a green and safe preparation method and biological applications of cerium phytate complexes with good thermal stability. Background Technology

[0002] The human body produces reactive oxygen species (ROS) and free radicals. Cellular tissues are oxidized by ROS and free radicals, causing various oxidative reactions in all macromolecules that make up cells, such as lipids, carbohydrates, proteins, and DNA. This leads to oxidative damage, including denaturation, cross-linking, and breakage, ultimately resulting in the destruction of cell structure and function, as well as tissue damage and organ disease. Currently, ROS and free radicals are responsible for over 100 diseases and aging processes (liver disease, hypertension, coronary heart disease, cancer formation and metastasis, kidney disease, etc.). To maintain normal bodily function and health, and to resist the damage of ROS and free radicals to tissues, the body needs antioxidants to inhibit their production. However, current antioxidants are not very effective and have low safety profiles.

[0003] Fatty liver disease is the most common liver disease affecting more than one-third of the world's population. It is caused by reactive oxygen species and free radicals in the body. In China, the prevalence of fatty liver disease is close to 30% and is increasing year by year, seriously endangering national health. Controlling energy intake, increasing exercise, and drug treatment can help alleviate fatty liver disease, but none of them can effectively cure it. Currently, there are no particularly effective drugs for reducing liver lipid damage. Existing adjuvant drugs such as lecithin, ursodeoxycholic acid, silymarin, inosine, coenzyme A, reduced glutathione, and taurine have not shown significant therapeutic effects on liver lipid damage.

[0004] Rare earth metals, due to their unique external electron configuration, possess special chemical and physical properties. Once inside an animal's body, they can be absorbed, accumulated, and redistributed within tissues, causing a wide range of effects on systems including the central nervous, digestive, endocrine, musculoskeletal, and reproductive systems. They influence intermediate metabolic processes through various mechanisms, such as regulating hormone levels, enzyme activity, and protein and lipid metabolism. Phytic acid, with its anti-inflammatory and antioxidant activities, holds promise for use as a combined antioxidant in humans, potentially treating liver damage caused by a high-fat diet. This project utilizes the antioxidant activity of cerium ions to coordinate with phytic acid to generate cerium phytate, effectively reducing reactive oxygen species and free radicals in the body. This, in turn, treats various diseases caused by reactive oxygen species and free radicals, particularly oxidative damage to the liver. Cerium phytate can remove excess lipids from the liver, inhibiting lipid damage at its source—a feat currently unattainable by existing drugs. Furthermore, cerium phytate exhibits excellent UV absorption capabilities; its UV absorption peak falls precisely in the most harmful UVB region, effectively shielding against ultraviolet radiation and reducing UV damage. Meanwhile, cerium phytate's excellent antioxidant activity can effectively eliminate UV-induced oxidative damage, reduce UV-induced skin damage, and lower the risk of skin cancer. Summary of the Invention

[0005] The purpose of this invention is to provide a method for preparing cerium phytate complexes and their biological applications, in order to solve the problems of poor efficacy and safety of existing antioxidants in treating diseases, the inability of existing drugs to treat lipid damage to the liver at its source, and the damage caused by ultraviolet radiation to the human body.

[0006] To achieve the above objectives, the present invention provides a method for preparing cerium phytate complexes, comprising the following steps:

[0007] (1) Solution preparation: Prepare aqueous solutions of cerium ions and phytic acid respectively;

[0008] (2) Preparation of cerium phytate complex: Add cerium ion aqueous solution to phytic acid aqueous solution under stirring, continue stirring until precipitation is complete, wash with ultrapure water until the washing solution is neutral, filter and dry to obtain cerium phytate complex with porous nanostructure.

[0009] Preferably, the cerium ion solution includes one or more of the following: cerium ammonium nitrate aqueous solution, cerium nitrate aqueous solution, and cerium chloride aqueous solution; and the phytic acid aqueous solution includes one or more of the following: phytic acid molecular aqueous solution, sodium phytate aqueous solution, and potassium phytate aqueous solution.

[0010] Preferably, the molar ratio of cerium ions in the cerium ion aqueous solution to phytic acid molecules or phytic acid ions in the phytic acid aqueous solution is (3-7):1.

[0011] Preferably, the application of cerium phytate complexes in the preparation of health supplements for the prevention or treatment of liver lipid damage. The health supplements use cerium phytate complexes as the main active ingredient.

[0012] Preferably, the use of cerium phytate complexes in the preparation of drugs for treating liver lipid damage. More preferably, the use of cerium phytate complexes in the preparation of drugs for treating liver damage caused by a high-fat diet.

[0013] Preferably, the use of compositions containing cerium phytate complexes in the preparation of medicaments for treating hepatic lipid injury.

[0014] The cerium phytate complex in this invention can be used alone, in combination with other drugs, or in combination with other drugs to form a compound preparation for treating lipid damage in the liver. All of these methods can achieve the purpose of treating lipid damage.

[0015] Preferably, the composition is a drug made by using cerium phytate complex as the active ingredient and pharmaceutically acceptable excipients.

[0016] The pharmaceutically acceptable excipients described in this invention refer to various conventional excipients required when preparing different dosage forms, such as diluents, binders, disintegrants, glidants, lubricants, flavoring agents, inclusion materials, adsorbents, etc., which are prepared into any commonly used oral dosage form using conventional formulation methods, such as granules, powders, tablets, capsules, pills, oral liquids, decoctions, and drop pills.

[0017] Preferably, cerium phytate complexes are used in the preparation of antioxidant foods and / or health products. These antioxidant foods and / or health products also include edible excipients.

[0018] Preferably, cerium phytate complexes are used in the preparation of antioxidant drugs.

[0019] Preferably, the dosage form of the antioxidant drug is selected from any one of capsules, tablets, powders, granules, injections, oral liquids, and pills. In practical applications, according to the actual needs of the drug dosage form, cerium phytate complexes are used as the active ingredient, and appropriate excipients are added to prepare the desired drug dosage form.

[0020] Preferred application of cerium phytate complexes in drugs for combating skin damage caused by ultraviolet radiation.

[0021] Preferred application of cerium phytate complexes in the preparation of sunscreens.

[0022] Preferred applications of cerium phytate complexes in drugs that reduce UV-induced photoaging, skin inflammation, and skin cancer.

[0023] Therefore, the preparation method and biological application of the cerium phytate complex with the above-described structure in this invention have the following beneficial effects:

[0024] 1. Cerium phytate is synthesized in an aqueous phase without the use of organic solvents. The synthesis process is safe and environmentally friendly. The only raw materials for synthesis are cerium salt and phytate. Phytate is derived from plant seeds and bran, making it green and safe. It is widely used in nutritional supplements, as well as for antioxidant and anti-tumor effects. The cerium phytate nanoparticles obtained by coordinating phytate with non-toxic cerium salt have good biosafety and can be used in a variety of life and health fields.

[0025] 2. Phytic acid, as a ligand molecule constituting CePA, has six phosphate groups, which can efficiently coordinate with cerium ions to form CePA materials. Phytic acid-cerium ion nanoparticles formed by the coordination of phytic acid and cerium ions carry a large amount of Ce. 3+ This is the basis for the antioxidant activity of cerium phytate. At the same time, the large number of pores inside the material effectively promotes the adsorption of reactive oxygen free radicals, giving it highly efficient antioxidant properties, which can be applied to the treatment of various diseases related to reactive oxygen species.

[0026] 3. Cerium phytate complexes possess excellent thermal stability and UV absorption capacity, making them suitable for use in UV-shielding materials and sunscreens. The good stability of cerium phytate allows for its application in coatings, building facades, glass, and pavements, helping to address UV protection needs in complex outdoor environments. Furthermore, the good biocompatibility and stability of cerium phytate also make it promising for skin UV protection, as it can absorb UV rays to reduce skin UV damage while simultaneously scavenging UV-induced reactive oxygen species, preventing UV damage to the skin.

[0027] 4. Phytate cerium complexes can also be effectively used in the treatment of liver lipid damage, effectively inhibiting fatty liver, reducing weight gain in high-fat mice, significantly reducing fat particles in the liver of mice, and effectively inhibiting liver damage caused by high-fat diet.

[0028] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0029] Figure 1 This is a molecular structure diagram of cerium phytate from Example 1 of the present invention;

[0030] Figure 2 This describes the appearance of cerium phytate in Example 1 of the present invention;

[0031] Figure 3 This is an electron microscope image of cerium phytate from Example 1 of the present invention;

[0032] Figure 4 This is the infrared absorption spectrum of cerium phytate from Example 1 of the present invention;

[0033] Figure 5 This is the ultraviolet absorption spectrum of cerium phytate in Example 1 of the present invention;

[0034] Figure 6 This is the nitrogen adsorption-desorption curve of cerium phytate in Example 1 of the present invention;

[0035] Figure 7 This is a pore size distribution diagram of cerium phytate in Embodiment 1 of the present invention;

[0036] Figure 8 This is the XPS spectrum of Embodiment 1 of the present invention;

[0037] Figure 9 This is a thermogravimetric diagram of Embodiment 1 of the present invention;

[0038] Figure 10 This is a graph showing the cytotoxicity test results of cerium phytate in Example 1 of the present invention;

[0039] Figure 11 This is a graph showing the test results of the antioxidant properties of cerium phytate in Example 1 of the present invention;

[0040] Figure 12 This is a diagram showing the results of cerium phytate scavenging intracellular reactive oxygen species generated by UVB in Example 1 of the present invention.

[0041] Figure 13 This is a diagram showing the results of cerium phytate in Example 1 of the present invention against UVB-induced skin damage;

[0042] Figure 14 This describes the effect of cerium phytate on mouse body size and liver in Example 1 of the present invention.

[0043] Figure 15 This describes the effect of cerium phytate on mouse body weight in Example 1 of the present invention.

[0044] Figure 16 This is a diagram showing the results of cerium phytate reducing liver inflammation and damage caused by a high-fat diet in Example 1 of this invention. Detailed Implementation

[0045] The present invention will be further described below. It should be noted that this embodiment is based on the present technical solution and provides detailed implementation methods and specific operation processes, but the present invention is not limited to this embodiment.

[0046] Example 1

[0047] Prepare 50 mL of 0.2 M cerium ammonium nitrate aqueous solution and 100 mL of 2% phytic acid molecular aqueous solution, respectively. Slowly add the cerium ammonium nitrate aqueous solution to the phytic acid molecular aqueous solution while stirring for 12 h. Then wash repeatedly with ultrapure water. After the supernatant of the washing solution is neutral, dry the material at 60 °C for later use.

[0048] Example 2

[0049] Prepare 100 mL of 0.1 M cerium nitrate aqueous solution and 100 mL of 2% sodium phytate aqueous solution, respectively. Slowly add the cerium nitrate aqueous solution to the sodium phytate aqueous solution while stirring for 12 h. Then wash repeatedly with ultrapure water. After the supernatant of the washing solution is neutral, dry the material at 60 °C for later use.

[0050] Example 3

[0051] Prepare 100 mL each of a 0.2 M cerium chloride aqueous solution and a 2% potassium phytate aqueous solution. Slowly add the cerium chloride aqueous solution to the potassium phytate aqueous solution while stirring for 12 hours. Then wash repeatedly with ultrapure water. After the supernatant of the washing solution is neutral, dry the material at 60°C for later use.

[0052] Example 4

[0053] The cerium phytate complex prepared in Example 1 was characterized.

[0054] Figure 1 For the molecular structure model of cerium phytate complex, from Figure 1 It can be seen that cerium ions coordinate with phosphate groups in phytic acid to form cerium phytate material, and cerium ions and phytic acid molecules form a porous nanostructure.

[0055] Figure 2 This is the appearance of the cerium phytate complex. The material is a pale yellow powder that can be uniformly dispersed in a solvent.

[0056] Figure 3 Electron micrograph of cerium phytate complex, from Figure 2 As can be seen from the data, the cerium phytate complex has a circular, plate-like structure with a particle size of about 50 nm and is relatively uniformly dispersed.

[0057] Figure 4 The infrared spectrum of the cerium phytate complex, from Figure 3 It can be seen from 1100cm -1 The presence of a characteristic peak nearby, corresponding to the characteristic peak of -PO-, proves that phytic acid rich in phosphate groups has coordinated with cerium ions, thus obtaining cerium phytate material.

[0058] Figure 5This is the UV spectrum of the cerium phytate complex. The material exhibits a significant UV absorption peak at 300 nm, corresponding to the UVB band of ultraviolet light (wavelength 280–320 nm). UVB light is the most damaging to the human body, making it the most important wavelength for sunscreens. Cerium phytate materials demonstrate good absorption properties in the UVB band and hold promise for applications in sunscreens and anti-photoaging materials.

[0059] Figure 6 and Figure 7 The figures show the nitrogen adsorption-desorption curves and pore size distribution of the cerium phytate complex. The figures show that the cerium phytate material has a certain adsorption and desorption capacity, indicating that the material has a large specific surface area. The cerium phytate material has a large number of micropores, which are about 10 nm in size. The micropores and the large specific surface area are conducive to the mass transfer of the substrate, which facilitates the subsequent in vivo treatment with cerium phytate material.

[0060] Figure 8 XPS spectrum of cerium phytate, from Figure 6 It can be seen from this that the cerium ions in the cerium phytate material are in a mixed valence state, Ce 3+ The proportion was 51.4%, with high Ce. 3+ The ratio helps to improve antioxidant performance.

[0061] Figure 9 The thermogravimetric analysis (TGA) of cerium phytate shows that cerium phytate does not decompose below 600 degrees Celsius, indicating that cerium phytate has good stability.

[0062] Example 5

[0063] The biosafety and antioxidant effects of the cerium phytate prepared in Example 1 were tested.

[0064] Biosafety testing

[0065] Biosafety testing procedure: Human hepatocyte suspension (1000 cells / well) was seeded into 96-well plates, and the plates were pre-cultured in an incubator for 12 h (37℃, 5% CO2). After pre-culture, the supernatant was discarded, and 100 μl of cerium phytate suspension (dispersed in culture medium) at different concentrations (0.1-1000 μg / mL) was added to each well, and the plates were incubated for 24 h. Finally, the supernatant was discarded, 100 μL of CCK-8 solution was added to each well, and the plates were incubated in an incubator for 2 h. The absorbance at 450 nm was then measured using a microplate reader, and cell viability was calculated.

[0066] from Figure 10 The cytotoxicity test results show that adding different concentrations of cerium phytate suspension has no significant effect on cell activity, indicating that cerium phytate material has good biosafety and can be used to prepare various formulations in biomedicine.

[0067] Antioxidant properties

[0068] Antioxidant activity assay procedure: Human hepatocyte suspension (1000 cells / well) was seeded into 96-well plates and pre-cultured in an incubator for 12 h (37℃, 5% CO2). After pre-culture, the supernatant was discarded, and 100 μl of 100 μg / mL cerium phytate suspension (dispersed in culture medium) was added to each well and incubated for 6 h. Subsequently, the supernatant was discarded, and 100 μL of H2O2 solution (0-10 mM) was added to each well and incubated in an incubator for 2 h. After H2O2 incubation, the supernatant was discarded, and 100 μL of CCK-8 solution was added to each well, and the 96-well plate was incubated in an incubator for 2 h. The absorbance at 450 nm was then measured using a microplate reader, and cell viability was calculated.

[0069] from Figure 11 The antioxidant performance test results show that cell activity decreases in the presence of hydrogen peroxide, and the decrease in cell activity becomes more pronounced with increasing hydrogen peroxide concentration. This is mainly because hydrogen peroxide has strong oxidizing properties and generates free radicals. Lipid peroxidation caused by free radicals plays a crucial role, directly causing oxidative damage to cells and reducing cell activity. When cerium phytate suspension was added, although cell activity decreased compared to the initial level, the cell activity after adding cerium phytate suspension was significantly greater than that after adding hydrogen peroxide solution. This indicates that cerium phytate material can effectively reduce H2O2-induced hepatocyte death and has good antioxidant properties. Therefore, cerium phytate material can be applied in antioxidant foods, health products, and pharmaceuticals to treat diseases caused by free radical oxidation in the body. Furthermore, considering the ultraviolet absorption capacity of cerium phytate… Figure 5 This material is also expected to be used in sunscreens and other fields to reduce the risk of diseases such as photoaging, skin inflammation and skin cancer caused by ultraviolet light.

[0070] Example 6

[0071] Experimental objective: To investigate the effects of cerium phytate complexes on UV-induced skin damage.

[0072] Experimental cells: Human skin stratum corneum cells (HaCaT)

[0073] Experimental animals: male mice, weighing (20±2)g

[0074] Experimental Methods: Cell and mouse UV damage models were established through UVB (300nm) irradiation. Cell Experiments: HaCaT cells were seeded into three 60mm culture dishes and pre-cultured for 12 hours (37℃, 5% CO2). The cells were divided into three groups: a control group, a UV irradiation group, and a cerium phytate + UV irradiation group. After pre-culture, the supernatant was discarded. A 100 μg / mL cerium phytate suspension (5 mL, dispersed in culture medium) was added to the cerium phytate + UV irradiation group and incubated for 6 hours. The other two groups were incubated with an equal volume of fresh culture medium for 6 hours. Subsequently, the UV group and the cerium phytate + UV group were treated with UVB light for 10 minutes each, followed by 2 hours of further incubation. Finally, the cells were stained with a dichlorodihydrofluoresceindiacetate dye, and the intracellular reactive oxygen species (ROS) levels were observed using a confocal microscope.

[0075] Animal experiments: Mice were divided into three groups: a control group, a UV light irradiation group, and a cerium phytate + UV light irradiation group. All mice in all three groups had their back hair removed with depilatory cream. The control group received no subsequent treatment. Mice in the UV light irradiation group were anesthetized and then irradiated with a UV lamp (UVB band) for 30 minutes twice daily for 7 days. Mice in the cerium phytate + UV light irradiation group had cerium phytate suspension (0.2 mg / mL) applied to their back skin before UV irradiation. After the cerium phytate suspension dried, the back skin was irradiated with a UV lamp (UVB band) for 30 minutes twice daily for 7 days. Finally, the mice were sacrificed, and the back skin tissue was separated. HE staining was used to observe the extent of skin damage.

[0076] Figure 12 Cellular reactive oxygen species (ROS) staining results showed that UV irradiation significantly increased intracellular ROS levels (green fluorescence). Skin cells pretreated with cerium phytate, after UV irradiation, exhibited significantly lower ROS levels (green fluorescence) than untreated UV-irradiated cells, approaching those of the control group, indicating that cerium phytate material possesses excellent antioxidant activity.

[0077] Figure 13 HE staining results of the skin showed that after UV irradiation, a large amount of inflammatory tissue appeared in the mouse skin tissue, and the skin cells were arranged in a disordered manner, indicating that UV irradiation causes skin inflammation and significant damage to skin tissue. However, the skin tissue treated with cerium phytate, after UV irradiation, showed intact skin structure and no obvious inflammatory manifestations, similar to the skin tissue of the control group mice. These results demonstrate that cerium phytate can be used to combat UV-induced skin damage.

[0078] Example 7

[0079] Experimental Objective: To investigate the effects of cerium phytate complexes on liver inflammatory damage induced by a high-fat diet.

[0080] Experimental animals: male mice, weighing (20±2)g

[0081] Experimental Methods: A mouse model of fatty liver was established by feeding mice with a high-fat diet. The control group was a normal diet (10% fat content). Mice in the high-fat diet group were fed a diet with 60% fat content. The cerium phytate intervention group received a high-fat diet (60% fat) supplemented with 1% CePA material by weight. All three types of mice were fed different diets under the same environment for 3 months. Afterwards, mouse weight was measured, and the mice were sacrificed, their livers were isolated, and liver tissue staining was performed to evaluate the protective effect of CePA against hepatic lipid damage.

[0082] from Figure 14 The comparison of mouse body size and liver size shows that the mice in the high-fat group are significantly larger than the mice in the control group and the cerium phytate intervention group. Compared with the mice fed a high-fat diet, the mice in the cerium phytate intervention group are significantly smaller in size, and no obvious fatty liver lesions are found in their livers. Figure 15 The asterisks in the diagram indicate significant differences between adjacent groups; three asterisks indicate highly significant differences. Figure 15 The graph showing the change in mouse body weight shows that the mice in the high-fat group gained a significant amount of weight under the high-fat diet. In contrast, the mice in the cerium phytate intervention group lost a significant amount of weight after adding cerium phytate to the high-fat diet, indicating that cerium phytate has a significant regulatory effect on lipid metabolism.

[0083] from Figure 16 The cell histological results show that the liver cells of mice in the high-fat group have extremely high fat content, severe cellular inflammation and damage, and severe liver fibrosis. Compared with the mice in the high-fat group, the mice in the cerium phytate intervention group showed a significant reduction in liver fat content, and the levels of inflammation and liver fibrosis were also significantly downregulated after adding cerium phytate to the high-fat diet. This indicates that cerium phytate can be used to reduce fat-induced liver damage.

[0084] Therefore, this invention employs a method for preparing cerium phytate complexes with the aforementioned structure and their biological applications. Cerium phytate is synthesized in an aqueous phase without the use of organic solvents, making the synthesis process safe and environmentally friendly. Phytic acid, as a ligand molecule constituting CePA, possesses six phosphate groups, enabling it to efficiently coordinate with cerium ions to form CePA materials, exhibiting excellent thermal stability and UV resistance. The cerium phytate nanoparticles formed by the coordination of phytic acid and cerium ions carry a large amount of Ce. 3+ This is the basis for the antioxidant activity of cerium phytate. At the same time, the numerous pores inside the material effectively promote the adsorption of reactive oxygen free radicals, giving it highly efficient antioxidant properties, which can be applied to the treatment of various diseases related to reactive oxygen species.

[0085] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. The application of cerium phytate complexes, characterized in that: Applications of cerium phytate complexes in the preparation of drugs to combat UV-induced skin damage, reduce UV-induced photoaging, skin inflammation, and skin cancer; The preparation method of cerium phytate complex includes the following steps: (1) Solution preparation: Prepare cerium ion aqueous solution and phytic acid aqueous solution respectively. The molar ratio of cerium ions in the cerium ion aqueous solution to phytic acid molecules or phytic acid ions in the phytic acid aqueous solution is (3-7):

1. (2) Preparation of cerium phytate complex: A cerium ion aqueous solution was added to a phytic acid aqueous solution under stirring. Stirring continued until precipitation was complete. The solution was washed with ultrapure water until neutral. After filtration and drying, a cerium phytate complex with a porous nanostructure was obtained. The cerium phytate complex exhibited a significant UV absorption peak at 300 nm. The cerium phytate complex had a porous nanostructure with 10 nm micropores and a particle size of 50 nm. The cerium ions in the cerium phytate complex were in a mixed valence state. 3+ The proportion is 51.4%.

2. The application according to claim 1, characterized in that: The cerium ion solution is selected from one or more of the following: cerium ammonium nitrate aqueous solution, cerium nitrate aqueous solution, and cerium chloride aqueous solution; the phytic acid aqueous solution is selected from one or more of the following: phytic acid molecular aqueous solution, sodium phytate aqueous solution, and potassium phytate aqueous solution.

3. The application of cerium phytate complexes, characterized in that: The use of a composition containing a cerium phytate complex in the preparation of a medicament for treating hepatic lipid injury, wherein the composition is a medicament made with a cerium phytate complex as the active ingredient and pharmaceutically acceptable excipients; The preparation method of cerium phytate complex includes the following steps: (1) Solution preparation: Prepare cerium ion aqueous solution and phytic acid aqueous solution respectively. The molar ratio of cerium ions in the cerium ion aqueous solution to phytic acid molecules or phytic acid ions in the phytic acid aqueous solution is (3-7):

1. (2) Preparation of cerium phytate complex: A cerium ion aqueous solution was added to a phytic acid aqueous solution under stirring. Stirring continued until precipitation was complete. The solution was washed with ultrapure water until neutral. After filtration and drying, a cerium phytate complex with a porous nanostructure was obtained. The cerium phytate complex exhibited a significant UV absorption peak at 300 nm. The cerium phytate complex had a porous nanostructure with 10 nm micropores and a particle size of 50 nm. The cerium ions in the cerium phytate complex were in a mixed valence state. 3+ The proportion is 51.4%.

4. The application of cerium phytate complexes, characterized in that: Application of cerium phytate complexes in the preparation of antioxidant drugs; The preparation method of cerium phytate complex includes the following steps: (1) Solution preparation: Prepare cerium ion aqueous solution and phytic acid aqueous solution respectively. The molar ratio of cerium ions in the cerium ion aqueous solution to phytic acid molecules or phytic acid ions in the phytic acid aqueous solution is (3-7):

1. (2) Preparation of cerium phytate complex: A cerium ion aqueous solution was added to a phytic acid aqueous solution under stirring. Stirring continued until precipitation was complete. The solution was washed with ultrapure water until neutral. After filtration and drying, a cerium phytate complex with a porous nanostructure was obtained. The cerium phytate complex exhibited a significant UV absorption peak at 300 nm. The cerium phytate complex had a porous nanostructure with 10 nm micropores and a particle size of 50 nm. The cerium ions in the cerium phytate complex were in a mixed valence state. 3+ The proportion is 51.4%.

5. The application according to claim 4, characterized in that: The dosage form of the antioxidant drug is selected from any one of capsules, tablets, powders, granules, injections, oral liquids, and pills.

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