Method for measuring content of artemisia leaf in artemisia leaf paper

Abstract

The present application relates to the detection of moxa, and particularly to a method for measuring the content of moxa in folium artemisiae. In order to solve the problem that the content of moxa in the moxa product cannot be quickly detected, especially the content of moxa in the new variety of moxa cannot be quickly detected in the research on the breeding and high-quality cultivation of moxa, the present application provides a method for measuring the content of moxa in folium artemisiae. The folium artemisiae is accurately weighed and then put into a strong alkali solution A with a mass fraction of 0.1%-30%, and is placed under the condition of 100-3000KHZ for ultrasonic treatment for 10-90 minutes. After the supernatant is filtered and removed, the residue is dried and weighed. The content of moxa = the weight of the dried residue / the weight of the folium artemisiae. The content of moxa in the moxa product can be quickly detected.

Description

TECHNICAL FIELD

[0001] The present application relates to the detection of moxa, and in particular to a method for measuring the content of moxa in folium artemisiae. BACKGROUND

[0002] Moxa is a soft and fine cotton-like product obtained by repeatedly drying, pounding, crushing, and sieving impurities and dust from folium artemisiae. Moxa is the main material for making moxa sticks and moxa products, and is an important indicator for measuring the quality of moxa products. However, due to the lack of effective methods for measuring the content of moxa in real work, the beating ratio is often used to measure the content of moxa in the production and sales process of moxa products, i.e. 1 kg of a certain moxa product is made from how many kg of folium artemisiae, such as the commonly seen 5:1, 8:1, 15:1, 30:1 products, which means that 1 kg of the product is made from 5, 8, 15, or 30 kg of folium artemisiae, respectively.

[0003] However, the content of moxa in folium artemisiae varies with different varieties, different cultivation methods, different origins, and different parts, and it is not objective to express the content of moxa in moxa products by the beating ratio, which may result in a large difference in the content of moxa in moxa products with the same beating ratio.

[0004] According to microscopic observation of folium artemisiae, the folium artemisiae used for making moxa can be divided into two parts, A and B. Part A is moxa, which is a mass of T-shaped non-glandular hairs entangled together; and part B is the remaining part without T-shaped non-glandular hairs, most of which are mesophyll cells.

[0005] T-shaped non-glandular hair cells are slender, curved or twisted, with a diameter of 4-20 μm and a length of 100-1000 μm. The two ends are tapered, and the middle part can be seen as a round or oval handle falling mark. The remaining ones are 1-6 square or rectangular cells, as shown in Figure 1 .

[0006] As the main component of moxa, T-shaped non-glandular hair can be observed under a microscope, but it requires higher professional knowledge and equipment, which is very time-consuming and laborious. SUMMARY

[0007] To solve the problem of being unable to quickly detect the content of moxa in moxa products, especially in the selection and breeding of new moxa varieties and the research on high-quality cultivation, the present application provides a method for measuring the content of moxa in folium artemisiae, which can quickly detect the content of moxa in moxa products.

[0008] To achieve the above-mentioned purpose, the present application is achieved in the following manner:

[0009] A method for measuring the content of moxa in Artemisia argyi, wherein the Artemisia argyi is accurately weighed and then put into a 0.1%-30% strong alkali solution A, and then ultrasonic treatment is carried out under the condition of 100-3000 KHZ for 10-90 minutes, and then the supernatant is filtered off, and then the residue is taken out, dried and weighed, and the content of moxa = the weight of the dried residue / the weight of the Artemisia argyi.

[0010] The method for measuring the content of moxa in Artemisia argyi, wherein the strong alkali solution A comprises NaOH or KOH or a mixture of the two in any ratio.

[0011] Preferably, the weight ratio of the strong alkali solution A to the Artemisia argyi is greater than 100:1.

[0012] The method for measuring the content of moxa in Artemisia argyi, wherein after the ultrasonic treatment under the condition of 300-3000 KHZ for 10-90 minutes, the supernatant is filtered off, and then the residue is washed with deionized water for 1-5 times, and then the residue is dried and weighed.

[0013] Compared with the prior art, the present application puts the moxa product into a 0.1%-30% strong alkali solution after the Artemisia argyi is accurately weighed, and then the insoluble substance-moxa is filtered and dried, and then weighed, so as to realize the rapid determination of the content of moxa in the moxa product, which is very intuitive and simple. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is a schematic diagram of T-shaped non-glandular villi under a microscope.

[0015] Figure 2 It is a micrograph of T-shaped non-glandular villi cells picked from the Artemisia argyi under a microscope.

[0016] Figure 3 It is a result diagram of the dried moxa dissolved by the method of the present application observed under a microscope.

[0017] Figure 4 It is a picture of the pre- and post-weighing of Example 1.

[0018] Figure 5 It is a picture of the pre- and post-weighing of Example 2. DETAILED DESCRIPTION

[0019] A method for measuring the content of moxa in Artemisia argyi, wherein the Artemisia argyi is accurately weighed and then put into a 0.1%-30% strong alkali solution A, and then ultrasonic treatment is carried out under the condition of 100-3000 KHZ for 10-90 minutes, and then the supernatant is filtered off, and then the residue is taken out, dried and weighed, and the content of moxa = the weight of the dried residue / the weight of the Artemisia argyi.

[0020] The weight ratio of the strong alkali solution A and the folium artemisiae argyi is greater than 10:1. Preferably, the weight ratio of the strong alkali solution A and the folium artemisiae argyi is greater than 100:1.

[0021] The strong alkali solution A comprises NaOH or KOH or a mixture of the two in any ratio.

[0022] The method for measuring the content of the folium artemisiae argyi in the folium artemisiae argyi comprises the following steps: after being placed under the condition of 300-3000 KHZ for 10-90 minutes of ultrasonic treatment, the supernatant is filtered out, and then the remaining material is washed with deionized water for 1-5 times, and then dried and weighed.

[0023] Compared with the prior art, the folium artemisiae argyi product is placed in a 0.1%-30% strong alkali solution after being accurately weighed, and the insoluble substance-folium artemisiae argyi is filtered and dried, and then weighed, so as to realize the rapid determination of the content of the folium artemisiae argyi product, which is very intuitive and simple.

[0024] In order to verify the purity of the folium artemisiae argyi in the insoluble substance in the present application, the product obtained by the method of the present application and the T-shaped non-glandular hair cells picked out from the crushed folium artemisiae argyi under a microscope are placed under a microscope for comparative microscopy, Figure 2 The micrograph of the T-shaped non-glandular hair cells picked out from the folium artemisiae argyi under a microscope, Figure 3 The result graph of the folium artemisiae argyi dissolved and dried by the method of the present application observed under a microscope shows that the purity of the T-shaped non-glandular hair cells in the insoluble substance is as high as 100%.

[0025] In order to verify the solubility of the folium artemisiae argyi, the T-shaped non-glandular hair cells picked out from the crushed folium artemisiae argyi (from the leaf blades of the folium artemisiae argyi from the Tanghe Tangai Ecological Agricultural Company in May 25, 2021) are placed in a 50℃ drying oven to dry to a constant weight, which is B1, and the weight is 2.120 mg. Then, B1 is treated according to the method of the present application, and the specific treatment method is as follows: B1 is placed in a 100ml NaOH solution with a mass fraction of 5%, and is placed under the condition of 300KHZ ultrasonic treatment for 40 minutes. After filtering out the supernatant, the remaining material is taken out and placed in a 50℃ drying oven to dry to a constant weight, which is B2, and the weight is 2.117 mg. The ratio of the weights before and after is B2 / B1, which is 99.86%. It shows that the method of the present application has low solubility of folium artemisiae argyi, and is reliable and effective for rapid detection of the content of folium artemisiae argyi in folium artemisiae argyi.

[0026] To verify the solubility of the application to different origin of moxa, at the same time, the T-shaped non-glandular cells picked out from the crushed leaves (randomly selected from Fudao Town, Tangyin County on May 30, 2021) under a microscope were placed in a 50℃ drying oven to dry to constant weight, as B3, the weight was 1.820mg, then B3 was treated according to the method of the application, the specific treatment method was: B3 was placed in 100ml of 0.1% NaOH solution, and was ultrasonicated under the condition of 3000KHZ for 90 minutes, then the supernatant was filtered off, and the remaining material was taken out and dried to constant weight in a 50℃ drying oven, as B4, the weight was 1.816mg. The ratio of the weights before and after: B4 / B3 was 99.78%. It showed that the method of the application had lower solubility of moxa, and was reliable and effective for rapid detection of the content of moxa in Artemisia leaf.

[0027] To verify the solubility of the application to different origin of moxa, at the same time, the T-shaped non-glandular cells picked out from the crushed leaves (randomly selected from Qiaogi County, Shaqi County on May 29, 2021) under a microscope were placed in a 50℃ drying oven to dry to constant weight, as B5, the weight was 1.6150mg, then B5 was treated according to the method of the application, the specific treatment method was: B5 was placed in 100ml of 10% NaOH solution, and was ultrasonicated under the condition of 3000KHZ for 30 minutes, then the supernatant was filtered off, and the remaining material was taken out and dried to constant weight in a 50℃ drying oven, as B6, the weight was 1.5970mg. The ratio of the weights before and after: B6 / B5 was 98.88%. It showed that the method of the application had lower solubility of moxa, and was reliable and effective for rapid detection of the content of moxa in Artemisia leaf.

[0028] The application can quickly and accurately determine the content of moxa in any artemisia product, and provides reliable data for objective evaluation of artemisia products.

[0029] The application will be specifically described below in conjunction with specific embodiments. It is necessary to point out here that the embodiments are only used to further illustrate the application and cannot be understood as limiting the protection scope of the application. Those skilled in the art can make some non-essential improvements and adjustments according to the content of the application.

[0030] Example 1:

[0031] An Artemisia leaf and moxa content determination method, comprising the following steps: the moxa products obtained by crushing the Artemisia leaves produced in different producing areas are all dried to constant weight and weighed, then are placed in 100ml of 7% NaOH solution, and are ultrasonicated under the condition of 1000KHZ for 40 minutes, then the supernatant is filtered off and the remaining material is washed with deionized water for 3 times, then the remaining material is dried to constant weight and weighed, and the content of moxa = the weight of the remaining material after drying / the weight of the Artemisia leaves.

[0032] Results are shown in Table 1 and Table 2 below. Figure 4

[0033] Table 1

[0034]

[0035] Example 2

[0036] A method for determining the content of moxa leaf moxa, comprising the following steps: different specifications of moxa leaf produced in different batches in Tanghe, Nanyang, are dried to constant weight, weighed, and then placed in 100ml of 10% NaOH solution, and then placed in 1000KHZ for ultrasonic treatment for 30 minutes, and then filtered to remove the supernatant, and then washed with deionized water for 5 times, and then dried to constant weight, weighed, and then the content of moxa is determined as the weight of the remaining material after drying / the weight of the moxa leaf.

[0037] Results are shown in Table 1 and Table 2 below. Figure 5

[0038] Table 2

[0039]

[0040] From the results, the results obtained after determination of the moxa leaf products containing different non-moxa components are also different, and the content of moxa determined by the present application is also low for the moxa leaf products containing more non-moxa components. Therefore, the present application is suitable for determining the content of moxa in different batches of moxa leaf products.

[0041] Example 3

[0042] A method for determining the content of moxa leaf moxa, comprising the following steps: different specifications of moxa leaf produced in different batches in Tanghe, Nanyang, are dried to constant weight, weighed, and then placed in 100ml of 30% NaOH solution, and then placed in 1000KHZ for ultrasonic treatment for 10 minutes, and then filtered to remove the supernatant, and then washed with deionized water for 7 times, and then dried to constant weight, weighed, and then the content of moxa is determined as the weight of the remaining material after drying / the weight of the moxa leaf.

[0043] Results are shown in Table 3 below.

[0044] Table 3

[0045]

[0046] For moxa leaf products containing different non-moxa components, the results obtained after determination by the present application are also different, and the content of moxa determined by the present application is also low for the moxa leaf products containing more non-moxa components. Therefore, the present application is suitable for determining the content of moxa in different batches of moxa leaf products.

[0047] ​​Example 4:

[0048] A method for determining the content of moxa leaf and moxa, comprising the following steps: moxa leaves produced in different producing areas are crushed to obtain moxa products with a ratio of 10:1 and 30:1 respectively, and then dried to a constant weight; the moxa products are respectively placed into 100ml of a NaOH solution with a mass fraction of 15%, and then ultrasonic treatment is performed under the condition of 1000KHZ for 20 minutes; after the supernatant is filtered out, the moxa products are washed with deionized water for 5 times; after the remaining products are dried to a constant weight, the content of moxa is determined according to the weight of the remaining products after drying / the weight of the moxa leaves.

[0049] The results are shown in the following table 4:

[0050] Table 4

[0051]

[0052] For moxa leaf products containing different non-moxa components from different producing areas, the results obtained by the method are also different. The content of moxa determined by the method is low for moxa leaf products containing more non-moxa components. Therefore, the method is suitable for determining the content of moxa in different batches of moxa leaf products.

[0053] In summary, the method can be used to determine the content of moxa leaf and moxa products from different producing areas.

[0054] The above only describes the preferred embodiments of the present application, but the protection scope of the present application is not limited thereto. It should be noted that, for those skilled in the art and any person skilled in the technical field, under the premise of not departing from the overall concept of the present application, according to the technical solution and the inventive concept of the present application, equivalent replacement or change, and several changes and improvements made, these should also be regarded as the protection scope of the present application.

Claims

1. A method for measuring the content of mugwort floss in mugwort leaves, characterized in that: After crushing the mugwort leaves, the resulting mugwort floss is dried to a constant weight and weighed. It is then placed in a 0.1%-30% strong alkaline solution A and sonicated at 100-3000 kHz for 10-90 minutes. The supernatant is filtered off, and the residue is removed, dried, and weighed. The mugwort floss content is calculated as: weight of the dried residue / weight of the mugwort leaves.

2. The method for measuring the content of Artemisia floss in Artemisia leaves according to claim 1, characterized in that: Strong alkaline solution A includes NaOH or KOH or a mixture of the two in any proportion.

3. The method for measuring the content of Artemisia floss in Artemisia leaves according to claim 1, characterized in that: The weight ratio of strong alkaline solution A to mugwort leaves is greater than 10:

1.

4. The method for measuring the content of Artemisia floss in Artemisia leaves according to claim 3, characterized in that: The weight ratio of strong alkaline solution A to mugwort leaves is greater than 100:

1.

5. The method for measuring the content of Artemisia floss in Artemisia leaves according to claim 1, characterized in that: After sonicating at 300-3000KHZ for 10-90 minutes, filter out the supernatant and then wash with deionized water 1-5 times. Dry the residue and weigh it.

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