Application of microbacteriocin and feed additive

Abstract

The present invention provides an application of microbacteriocin and a feed additive. By using microbacteriocin to inhibit Clostridium perfringens, specifically by making microbacteriocin into a feed additive for use in feed, it can effectively prevent avian necrotic enteritis, thereby reducing the mortality rate of poultry.

Description

Technical Field

[0001] The present invention relates to the technical field of feed additives, in particular to feed additives for poultry, and specifically to the application of microcins and feed additives. Background Art

[0002] Necrotic enteritis (NE) caused by Clostridium perfringens is one of the most common diseases in global poultry production. Acute NE mainly causes rapid death of diseased poultry, with a mortality rate as high as 50%; subclinical NE will cause listlessness and decreased production performance in broilers, etc. Clostridium perfringens is a common bacterium in the avian intestine. When the external environment is interfered by factors such as diet structure (such as high non-starch polysaccharide, high-protein diet, etc.), microorganisms (such as coccidia, etc.), stress, etc., Clostridium perfringens will multiply in large numbers, leading to the occurrence of necrotic enteritis. In addition, Clostridium perfringens also interacts with coccidia, leading to the occurrence of necrotic enteritis.

[0003] In response to NE in poultry, the industry generally uses antibiotics (such as enramycin) added to feed for prevention. With the increasingly strict management of additive drugs in China, it is difficult for feed enterprises and breeding enterprises to find effective and economical ways to control diseases caused by Clostridium perfringens. Although vaccines against Clostridium perfringens have long been on the market, the immunization cost is high, and there are deficiencies in safety, residual toxicity, efficacy, etc. Therefore, after antibiotics are withdrawn from feed, feed enterprises and breeding enterprises do not have more effective and economical ways to control Clostridium perfringens.

[0004] In summary, there is an urgent need for an application of microcins and feed additives to solve the problems existing in the prior art. Summary of the Invention

[0005] The purpose of the present invention is to provide an application of microcins and feed additives, and the specific technical solutions are as follows:

[0006] Application of microcins in preventing avian necrotic enteritis.

[0007] Preferably, the MIC concentration of the microcins is 4 μg / ml.

[0008] The present invention also provides an application of microcins in feed additives.

[0009] The present invention also provides a feed additive with the function of preventing avian necrotic enteritis. The feed additive includes 1 - 10 parts by mass of microcins and 90 - 99 parts by mass of a carrier.

[0010] Preferably, the carrier includes corn starch, tapioca starch, wheat bran or defatted rice bran.

[0011] Applying the technical solution of the present invention has the following beneficial effects:

[0012] The use of microcins in the present invention to inhibit Clostridium perfringens can effectively prevent avian necrotic enteritis, thereby reducing the mortality rate of poultry.

[0013] In addition to the purposes, features and advantages described above, the present invention has other purposes, features and advantages. The present invention will be further described in detail below. Detailed implementation mode

[0014] To facilitate the understanding of the present invention, the present invention will be described more comprehensively below, and preferred embodiments of the present invention are given. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0015] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present invention belongs. The terms used in the description of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0016] Example 1:

[0017] Example 1: Effect of microcin (Mcc J25) on inhibiting Clostridium perfringens

[0018] Take 8 sterilized 250 ml Erlenmeyer flasks containing 50 ml of Clostridium proliferation medium. After mixing different concentrations of microcin (MccJ25) with Clostridium perfringens, the final concentration of microcin is 0, 1, 2, 4, 8, 16, 32, 64 μg / ml, and the initial number of Clostridium perfringens is 1.0×10 6 cfu / ml. After culturing in an anaerobic incubator at 37°C for 24 h, the number of Clostridium perfringens is detected. The number of Clostridium perfringens is as follows:

[0019] Table 1 Number of Clostridium perfringens

[0020]

[0021] Judging from the experimental results, the initial number of Clostridium perfringens is 1.0×10 6 cfu / ml. After 24 h of culture in the Erlenmeyer flask without adding MccJ25, the Clostridium perfringens proliferated to 2.5×10 9cfu / ml; When the concentration of MccJ25 reached 2 μg / ml, compared with the control group, Clostridium perfringens basically did not proliferate; when the concentration of MccJ25 reached 8 μg / ml, compared with the control group, all Clostridium perfringens died; it indicated that the MIC concentration (minimum inhibitory concentration) of MccJ25 inhibiting Clostridium perfringens was 4 μg / ml.

[0022] Example 2: Application effect of Mcc J25 in preventing necrotic enteritis in broilers

[0023] The feed additive for preventing necrotic enteritis in broilers is composed of the following components by mass: 1 part of microcin (Mcc J25) and 99 parts of corn starch, and they are evenly mixed in proportion. The above feed additive is added to broiler feed at a certain concentration.

[0024] 300 one-day-old AA male broilers were selected and randomly divided into 5 treatments, with 6 replicates for each treatment and 10 broilers for each replicate. The experiment was set with 5 treatments respectively. Negative control group: fed with basal diet, not challenged; positive control group: fed with basal diet, challenged with Clostridium perfringens; experimental group I (16 mg / kg enramycin); experimental group II (10 mg / kg microcin); experimental group III (20 mg / kg microcin) (Table 1). Broilers in the positive control group and experimental groups were challenged with coccidia at 14 days old and challenged with Clostridium perfringens at 19 - 21 days old. The broilers were raised in cages, with free access to food and water, and the experimental period was 42 days. The intestinal scoring criteria are shown in Table 3, and the experimental results are shown in Table 4.

[0025] Table 2 Experimental design

[0026] Grouping Diet Negative control group Basal diet, not challenged Positive control group Basal diet, challenged with Clostridium perfringens Experimental group I Basal diet + 16 mg / kg enramycin, challenged with Clostridium perfringens Experimental group II Basal diet + 10 mg / kg microcins, challenged with Clostridium perfringens Experimental group III Basal diet + 20 mg / kg microcins, challenged with Clostridium perfringens

[0027] Table 3 Intestinal injury scoring criteria

[0028]

[0029] Table 4 Effect of MccJ25 on intestinal lesion scores of broilers challenged with Clostridium perfringens

[0030]

[0031]

[0032] As shown in Table 4, at 28 days of age: compared with the negative control group, the positive control group significantly increased the lesion scores of the duodenum, jejunum, ileum and cecum of broilers at 28 days of age (P<0.001). Compared with the positive control group, the experimental groups all reduced the lesion score of the jejunum of broilers at 35 days of age (P<0.01). Compared with the positive control group, experimental group I (added 16 mg / kg enramycin) reduced the lesion score of the ileum of broilers at 35 days of age (P<0.05); compared with the positive control group, experimental group II (added 10 mg / kg microcins) and experimental group III (added 20 mg / kg microcins) both extremely significantly reduced the lesion score of the cecum of broilers at 35 days of age (P<0.001);

[0033] At 35 days of age: compared with the negative control group, the positive control group significantly increased the lesion scores of the duodenum and jejunum of broilers at 35 days of age (P<0.01), and significantly increased the lesion scores of the ileum and cecum of broilers at 35 days of age (P<0.001). Compared with the positive control group, experimental group I (added 16 mg / kg enramycin), experimental group II (added 10 mg / kg microcins), and experimental group III (added 20 mg / kg microcins) all extremely significantly reduced the lesion scores of the duodenum, jejunum, ileum and cecum of challenged broilers (P<0.001). It shows that under the condition of Clostridium perfringens challenge, using microcins can effectively treat the diseases caused by Clostridium perfringens during the production period, and can be used as an alternative drug to enramycin, and has a significant therapeutic effect on broiler clostridial enteritis.

[0034] The above are only the preferred embodiments of the present invention and are not used to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. Application of microbicin Mcc J25 in preparing feed additive for preventing necrotic enteritis in broilers.

2. Use of microcin Mcc J25 according to claim 1 in the preparation of a feed additive for preventing necrotic enteritis in broiler chickens, characterized in that, The MIC concentration of the microbicin is 4 μg / ml.

3. Use of microcin Mcc J25 according to claim 1 in the preparation of a feed additive for preventing necrotic enteritis in broiler chickens, characterized in that, It has the function of preventing avian necrotic enteritis. The feed additive comprises 1-10 parts by mass of microbicin and 90-99 parts by mass of carrier.

4. Use of microcin Mcc J25 according to claim 3 in the preparation of a feed additive for preventing necrotic enteritis in broiler chickens, characterized in that, The carrier includes corn starch, tapioca starch, wheat bran or defatted rice bran.

Images