Microorganism separation device and microorganism separation method

Abstract

The present invention relates to the field of biochemical analysis technology, and provides a microorganism separation device and a microorganism separation method. The microorganism separation device includes: a magnetic field generating unit, the magnetic field generating unit includes two magnets with the same poles arranged opposite to each other, and can generate a magnetic field on the outside of the two magnets; a microfluidic chip, the microfluidic chip is arranged on the outside of the two magnets, and a fluid channel is provided on the microfluidic chip, and the fluid channel is used for passing fluid and sheath flow, wherein the fluid includes a complex of magnetic particles and target microorganisms, magnetic particles and non-magnetic substances. In an embodiment of the present invention, the fluid can flow under the action of a magnetic field. Under the influence of the magnetic field, the complex containing magnetic substances in the fluid will deviate from the original direction of movement. By controlling the flow rate of the fluid and the sheath flow, the complex can be automatically separated from the fluid, thereby avoiding the operation of manual separation, improving the separation speed, and also increasing the sample processing capacity.

Claims

1. A microorganism separation device, characterized in that: include: A magnetic field generating unit, comprising two magnets with the same poles facing each other, capable of generating a magnetic field outside the two magnets; A microfluidic chip, the microfluidic chip being disposed outside the two magnets, the microfluidic chip being provided with a fluid channel for passing fluid and sheath flow, wherein the fluid includes a complex of magnetic particles and target microorganisms, magnetic particles, and non-magnetic substances; The magnetic field generating unit further includes: a reinforcement member, the reinforcement member being arranged between the two magnets, and the magnets and the reinforcement member both being in a circular shape; Wherein, the outer diameter of the reinforcement is larger than the outer diameter of the magnet, The microfluidic chip is arranged in the middle position of the reinforcement.

2. The microorganism separation device according to claim 1, characterized in that A plurality of serrations arranged along the circumferential direction are provided on the outer wall of the reinforcement.

3. The microorganism separation device according to claim 1, characterized in that Also includes: A driving member is used to drive the magnetic field generating unit to rotate.

4. The microorganism separation device according to claim 3, characterized in that The driving member includes: Motor; A transmission shaft, wherein a first end of the transmission shaft is connected to the output shaft of the motor, a second end of the transmission shaft is detachably connected to the magnetic field generating unit, and the transmission shaft can drive the magnetic field generating unit to rotate.

5. The microorganism separation device according to claim 3 or 4, characterized in that: The microfluidic chip is provided with a through hole, and the microfluidic chip is mounted on the magnetic field generating unit based on the through hole, and a preset gap is provided between the through hole and the magnetic field generating unit.

6. The microorganism separation device according to claim 5, characterized in that: The fluid channel includes an inlet channel, a surrounding channel and an outlet channel. The inlet channel, the surrounding channel and the outlet channel are connected in sequence, the surrounding channel is arc-shaped, and the surrounding channel is arranged around the through hole.

7. The microorganism separation device according to claim 6, characterized in that: The end of the inlet channel is provided with: an inner inlet close to the through hole and an outer inlet away from the through hole; The end of the outflow channel is provided with an inner outlet close to the through hole and an outer outlet away from the through hole.

8. A method for separating microorganisms, characterized in that: The microorganism separation device system according to any one of claims 1 to 7, wherein the microorganism separation method comprises: introducing a fluid and a sheath flow into the fluid channel, wherein the fluid includes a complex of magnetic particles and target microorganisms, magnetic particles, and non-magnetic substances, wherein the sheath flow is used to cause the particles in the fluid to flow in a single file; controlling the introduced fluid and the sheath flow to flow at a first flow rate, and separating the complex and the magnetic particles from the fluid under the action of a corresponding magnetic field, and / or, The introduced fluid and the sheath flow are controlled to flow at a second flow rate, and the complex is separated from the magnetic particles under the action of a corresponding magnetic field.

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