A vibrating cell transfection microscopy observation platform

Abstract

The application discloses a vibrating cell transfection microscopic observation platform, which comprises a sample loading module, a vibrating module and an observation module, wherein the sample loading module comprises a bottom plate and a sample stage installed on the bottom plate, and the vibrating module and the observation module are arranged on the bottom plate. The vibrating module comprises a driving motor and a sample table connected with the driving motor through an eccentric rotating shaft, and the sample table is used for placing a micro-fluidic chip. The module comprises a microscope tube, a high-speed camera assembled above an observation lens and a light source located below the observation lens. The driving motor rotates at a high speed, drives the sample table to make reciprocating motion through the eccentric rotating shaft, and makes the micro-fluidic chip move with the sample table; a suitable imaging focal plane is found by adjusting the height of the microscope tube; the light source illuminates the high-speed camera; and the high-speed camera collects signals to clearly record the flow channel morphology of the micro-fluidic chip and the internal cell motion state and trajectory, so that the accurate observation of the motion state of the cell micro-mechanical behavior is realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cell microscopic observation technology, and particularly relates to a vibrating cell transfection microscopic observation platform. BACKGROUND

[0002] Microfluidic technology provides great potential for the development of cell biology and clinical applications. Single cell analysis and single cell manipulation based on microfluidic chips provide a powerful means for quantitative and precise study of cell behavior. To achieve efficient cell manipulation of microfluidic chips, it is necessary to determine the microscale motion behavior of cells under different forms of force. However, there is currently a lack of effective means to observe the mechanical behavior of cells under external vibration, and therefore it is not possible to accurately observe the motion of cells under high-frequency external vibration. SUMMARY

[0003] Therefore, the technical problem to be solved by the present application is the defect that the cell research observation platform in the prior art cannot accurately observe the microfluidic motion of cells under high-frequency external vibration, so as to provide a vibrating cell transfection microscopic observation platform.

[0004] To solve the above technical problems, the technical scheme of the present application is as follows:

[0005] A vibrating cell transfection microscopic observation platform, comprising:

[0006] A sample loading module comprising a base plate and a sample stage mounted on the base plate;

[0007] A vibration module comprising a drive motor arranged on the base plate, a transmission assembly connected to the output end of the drive motor and located above the sample stage, and a sample stage connected to the transmission assembly; the drive motor drives the sample stage and the microfluidic chip placed thereon to do linear reciprocating motion through the transmission assembly;

[0008] An observation module comprising an observation mirror located above the sample stage, a high-speed camera arranged above the observation mirror, and a light source mounted on the base plate and located below the observation mirror; the sample stage is located between the observation mirror and the light source.

[0009] Further, a guide rail is fixed above the sample stage; the transmission assembly comprises an eccentric shaft coaxially connected to the drive motor through a shaft coupling, a transmission rod connected to the eccentric shaft, one end of the transmission rod doing eccentric motion with the rotation of the eccentric shaft; the transmission assembly further comprises a sliding block connected to the other end of the transmission rod and linearly slidingly connected to the guide rail, and the sample stage is connected to the sliding block to do linear reciprocating motion with the sliding block.

[0010] Further, the support base is arranged on the bottom plate through a support rod, and a fixed plate is fixed on the upper surface of the support base, and the guide rail is fixed on the fixed plate.

[0011] Further, the fixed plate is fixedly installed on the support base through screws, and a through hole is arranged on the support base and the fixed plate, and the two through holes are communicated, and the observation mirror, the light source, the sample table and the through hole are located on the same straight line.

[0012] Further, a support frame is fixed on the support rod, and the support base is arranged on the support frame, and the support base can slide along the X-axis and the Y-axis in the horizontal direction.

[0013] Further, mounting holes are arranged at two ends of the transmission rod, the eccentric shaft is connected to one end of the transmission rod through a first deep groove ball bearing arranged in the mounting hole, the slider is fixed on a second deep groove ball bearing arranged in the other mounting hole, the sample table is in the shape of 'Z', one end of the sample table is located between the observation mirror and the light source, and the other end of the sample table is connected to the other end of the transmission rod through the second deep groove ball bearing.

[0014] Further, a first clamping groove is arranged on the sample table, and an elastic clamping spring suitable for limiting the second deep groove ball bearing is arranged in the first clamping groove, and a second clamping groove is arranged on the eccentric shaft, and a stepped shaft suitable for limiting the first deep groove ball bearing is arranged in the second clamping groove.

[0015] Further, the observation module further comprises a focusing device installed on the bottom plate, the observation mirror comprises a microscope tube threadedly connected with the focusing device and a long-distance objective lens connected with the microscope tube, and the long-distance objective lens is located directly above the light source.

[0016] Further, a fixing ring is slidably arranged on the focusing device, the microscope tube is threadedly connected with the fixing ring, the long-distance objective lens is connected with the long-distance objective lens through an adapter ring, the light source is arranged on the bottom plate through a support, the illumination intensity of the light source is adjustable, and the support can be adjusted in lifting on the bottom plate.

[0017] Further, the driving motor is a direct current motor.

[0018] The technical scheme of the present application has the following advantages:

[0019] 1. The vibration cell transfection microscopic observation platform provided by the present application is characterized in that a vibration module and an observation module are arranged on the bottom plate of the object module, the vibration module comprises a driving motor and a sample table connected with the driving motor through a transmission assembly and adapted to place a microfluidic chip, the driving motor rotates at a high speed, drives the sample table to make linear reciprocating motion through the transmission assembly, and simultaneously makes the liquid contained in the microfluidic chip oscillate along with the motion of the sample table, so that the cells in the liquid are caused to move; the observation module comprises an observation lens, a high-speed camera arranged above the observation lens, and a light source installed on the bottom plate and located below the observation lens, the light source can illuminate the high-speed camera, and the high-speed camera collects image signals on the observation lens, so that the morphology of the microfluidic channel containing the liquid on the microfluidic chip and the motion state and trajectory of the cells inside the liquid can be clearly observed, and the motion of the cells in the microfluidic channel can be accurately observed.

[0020] 2. The vibration cell transfection microscopic observation platform provided by the present application is characterized in that a guide rail is fixed above the object table; the transmission assembly comprises an eccentric rotating shaft coaxially connected with the driving motor through a shaft coupling and a transmission rod connected with the eccentric rotating shaft, one end of the transmission rod makes eccentric motion along with the rotation of the eccentric rotating shaft; the transmission assembly further comprises a sliding block connected with the other end of the transmission rod and linearly slidably connected with the guide rail, and the sample table is connected with the sliding block and makes linear reciprocating motion along with the sliding block. In this way, the cooperation of the sliding block and the guide rail can realize low-friction and high-precision directional motion, and the sample table can make linear reciprocating motion along the guide rail under the geometric constraint of the sliding block and the guide rail, so as to ensure the regular motion of the cells inside the liquid contained in the microfluidic chip.

[0021] 3. The vibration cell transfection microscopic observation platform provided by the present application is characterized in that the object table is arranged on the bottom plate through a support rod, an upper surface of the object table is fixed with a fixed plate, and the guide rail is fixed on the fixed plate. In this way, the stability of the sliding block when sliding on the fixed guide rail can be ensured.

[0022] 4. The vibration cell transfection microscopic observation platform provided by the present application is characterized in that the fixed plate is fixedly installed on the object table through screws, through holes are formed in the object table and the fixed plate and are communicated with each other, and the observation lens, the light source and the sample table are located on the same straight line as the through holes. In this way, the liquid contained in the microfluidic chip can be observed.

[0023] 5. The vibration cell transfection microscopic observation platform provided by the present application is characterized in that a support frame is fixed on the support rod, the object table is arranged on the support frame, and the object table can slide along the X-axis and the Y-axis in the horizontal direction. In this way, the microfluidic chip can be conveniently adjusted to a position relative to the observation lens.

[0024] 6. The vibration cell transfection microscopic observation platform provided by the present application, both ends of the transmission rod are provided with mounting holes, and deep groove ball bearings are arranged in the two mounting holes; the eccentric rotating shaft is connected with one end of the transmission rod through the first deep groove ball bearing; the sample table is in the shape of "Z", one end of the sample table is located between the observation lens and the light source, and the other end of the sample table is connected with the other end of the transmission rod through the second deep groove ball bearing. In this way, the friction between the transmission rod and the eccentric rotating shaft and the sample table can be reduced, and the rotating fit of the connection between the transmission rod and the eccentric rotating shaft and the sample table is ensured.

[0025] 7. The vibration cell transfection microscopic observation platform provided by the present application, the first clamping groove is arranged on the sample table, and an elastic clamping spring suitable for limiting the second deep groove ball bearing is arranged on the first clamping groove; the second clamping groove is arranged on the eccentric rotating shaft, and a stepped shaft suitable for limiting the first deep groove ball bearing is arranged on the second clamping groove. In this way, the first deep groove ball bearing can be limited by the elastic clamping spring, the connection stability between the transmission rod and the sample table is ensured, and at the same time, axial movement between the transmission rod and the sample table along the first deep groove ball bearing is avoided, so that the movement stability of the sample table is maintained; the second deep groove ball bearing is limited by the stepped shaft, the connection stability between the transmission rod and the eccentric rotating shaft is ensured, and at the same time, slippage between the transmission rod and the eccentric rotating shaft during work is avoided when they are connected.

[0026] 8. The vibration cell transfection microscopic observation platform provided by the present application, the observation module further comprises a focusing device mounted on the bottom plate; the observation lens comprises a microscope tube threadedly connected with the focusing device and a long-distance objective lens connected with the microscope tube; and the long-distance objective lens is located directly above the light source. In this way, the high-speed camera can be provided with appropriate magnification, and at the same time, the relative position between the long-distance objective lens and the microfluidic chip can be controlled through the focusing device, so that the microfluid channel morphology and the internal cell movement state can be clearly observed.

[0027] 9. The vibration cell transfection microscopic observation platform provided by the present application, the light source is arranged on the bottom plate through a support, the light source illumination intensity is adjustable, and the support can be adjusted in lifting on the bottom plate. In this way, the light source can be adjusted to an appropriate height, and at the same time, the high-speed camera can be provided with appropriate illumination intensity through appropriate adjustment of the light source intensity. BRIEF DESCRIPTION OF DRAWINGS

[0028] In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the drawings needed in the following specific embodiments or prior art description will be briefly introduced. Obviously, the drawings in the following description are some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0029] Figure 1A three-dimensional structural diagram of the vibrating cell transfection microscopic observation platform provided in an embodiment of the present invention;

[0030] Figure 2 This is a three-dimensional structural diagram of the vibration module in this invention;

[0031] Figure 3 This is a schematic diagram showing the connection relationship between the sample stage and the eccentric rotating shaft in this invention;

[0032] Figure 4 This is a three-dimensional structural diagram of the sample stage in this invention;

[0033] Figure 5 This is a three-dimensional structural diagram of the eccentric rotating shaft in this invention.

[0034] Explanation of reference numerals in the attached figures: 1. High-speed camera; 2. Microscope tube; 3. Fixing ring; 4. Long-distance objective lens; 5. Fixing plate; 6. Stage; 7. Support frame; 8. Support rod; 9. Light source; 10. Support; 11. Base plate; 12. DC motor; 13. Fixing frame; 14. Coupling; 15. Focusing device; 16. Second deep groove ball bearing; 17. Transmission rod; 18. Slider; 19. Sample stage; 20. Guide rail; 21. Screw; 22. Eccentric rotating shaft; 23. Microfluidic chip. Detailed Implementation

[0035] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0037] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0038] Furthermore, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0039] like Figures 1-5 The illustrated vibrating cell transfection microscopy observation platform includes a sample carrier module, a vibration module, and an observation module. The sample carrier module includes a base plate 11 and a stage 6 mounted on the base plate 11. The vibration module includes a drive motor mounted on the base plate 11, a transmission assembly connected to the output of the drive motor and located above the stage 6, and a sample stage 19 connected to the transmission assembly. The drive motor drives the sample stage 19 and the microfluidic chip 23 placed on it to perform linear reciprocating motion via the transmission assembly. The observation module includes an observation mirror located above the sample stage 19, a high-speed camera 1 mounted above the observation mirror, and a light source 9 mounted on the base plate 11 and located below the observation mirror. The sample stage 19 is located between the observation mirror and the light source 9.

[0040] This vibrating cell transfection microscopy observation platform has a vibration module and an observation module mounted on the base plate 11 of the material carrier module. The vibration module includes a drive motor and a sample stage 19 connected to the drive motor via a transmission component and adapted to place the microfluidic chip 23. The motor shaft of the drive motor rotates at high speed, driving the sample stage 19 to perform linear reciprocating motion via the transmission component. At the same time, the liquid contained in the microfluidic chip 23 oscillates with the movement of the sample stage 19, thereby causing the cells in the liquid to move. The observation module includes an observation mirror, a high-speed camera 1 mounted above the observation mirror, and a light source 9 mounted on the base plate 11 and located below the observation mirror. The light source 9 illuminates the high-speed camera 1, and the high-speed camera 1 uses CCD imaging to collect image signals from the observation mirror, thereby clearly observing the microchannel morphology of the liquid contained in the microfluidic chip 23 and the internal cell movement state and trajectory, thus achieving precise observation of the cell movement under micro-mechanical behavior.

[0041] In this embodiment, the drive motor is specifically a DC motor 12. A guide rail 20 is fixed above the stage 6; the transmission assembly includes an eccentric shaft 22 coaxially connected to the DC motor 12 via a coupling 14, and a transmission rod 17 connected to the eccentric shaft 22. One end of the transmission rod 17 moves eccentrically with the rotation of the eccentric shaft 22; the transmission assembly also includes a slider 18 connected to the other end of the transmission rod 17 and linearly slidably connected to the guide rail 20. The sample stage 19 is connected to the slider 18 and moves linearly reciprocatingly with the slider. With this configuration, the cooperation between the slider and the guide rail can achieve low-friction, high-precision directional movement, thereby allowing the sample stage to move linearly reciprocally along the guide rail under the geometric constraints of the slider and the guide rail, thus ensuring the regular movement of the internal cells containing liquid on the microfluidic chip. Specifically, the stage 6 is mounted on the base plate 11 via a support rod 8, and a fixing plate 5 is fixed to the upper surface of the stage 6, with the guide rail 20 fixed to the fixing plate 5. This configuration ensures the stability of the slider 18 when it slides on the fixed guide rail 20.

[0042] In this embodiment, both the stage 6 and the fixing plate 5 have through holes, and the two through holes are interconnected. The observation mirror, light source 9, sample stage 19, and through holes are all located on the same straight line. This arrangement ensures that the liquid contained in the microfluidic chip 23 can be observed. A support frame 7 is vertically fixed on the support rod 8, and the stage 6 is mounted on the support frame 7. The stage 6 can slide horizontally along the X and Y axes. This arrangement facilitates the adjustment of the microfluidic chip 23 relative to the observation mirror. Specifically, the flow channel structure within the microfluidic chip 23 can be customized according to research needs, and cell suspensions can be loaded into the flow channels.

[0043] In this embodiment, mounting holes are provided at both ends of the transmission rod 17, and deep groove ball bearings 16 are installed in both mounting holes. The eccentric shaft 22 is connected to one end of the transmission rod 17 through a first deep groove ball bearing. Specifically, the sample stage 19 is Z-shaped, with one end of the sample stage 19 located between the observation mirror and the light source 9, used to place the microfluidic chip 23; the other end of the sample stage 19 is connected to the other end of the transmission rod 17 through a second deep groove ball bearing 16. Specifically, the eccentric shaft 22 mates with the inner ring of the first deep groove ball bearing, and one end of the transmission rod 17 mates with the outer ring of the first deep groove ball bearing. The sample stage 19 mates with the inner ring of the second deep groove ball bearing 16, and the other end of the transmission rod 17 mates with the outer ring of the second deep groove ball bearing 16. This arrangement reduces friction between the transmission rod 17, the eccentric shaft 22, and the sample stage 19, ensuring rotational engagement at the connection between the transmission rod 17, the eccentric shaft 22, and the sample stage 19.

[0044] Specifically, the sample stage 19 is provided with a first retaining groove, on which an elastic retaining spring is provided to limit the positioning of the second deep groove ball bearing 16; the eccentric shaft 22 is provided with a second retaining groove, on which a stepped shaft is provided to limit the positioning of the first deep groove ball bearing 16. This arrangement allows the elastic retaining spring to limit the positioning of the deep groove ball bearing, ensuring the connection stability between the transmission rod 17 and the sample stage 19, while preventing axial movement between the transmission rod 17 and the sample stage 19 along the first deep groove ball bearing, thus maintaining the motion stability of the sample stage 19; the stepped shaft limits the positioning of the second deep groove ball bearing 16, ensuring the connection stability between the transmission rod 17 and the eccentric shaft 22, while preventing slippage during operation when the transmission rod 17 and the eccentric shaft 22 are connected.

[0045] In this embodiment, the observation module further includes a focusing device 15 vertically fixed on the base plate 11; the observation lens includes a microscope tube 2 threadedly connected to the focusing device 15 and a long-distance objective lens 4 connected to the microscope tube 2; the long-distance objective lens 4 is located directly above the light source 9. This arrangement can provide a suitable magnification for the high-speed camera 1, while the focusing device 15 can control the relative position of the long-distance objective lens 4 and the microfluidic chip 23, thereby ensuring clear observation of the microchannel morphology and internal cell movement.

[0046] Specifically, a fixing ring 3 is slidably mounted on the focusing mechanism 15, and the microscope tube 2 is threadedly connected to the fixing ring 3. The long-distance objective lens 4 is connected to the long-distance objective lens 4 via an adapter ring; the light source 9 is mounted on the base plate 11 via a bracket 10, and the illumination intensity of the light source 9 is adjustable. The bracket 10 can be raised and lowered on the base plate 11. With this configuration, the light source 9 can be adjusted to an appropriate height via the height-adjustable bracket 10, and the intensity of the light source 9 can be appropriately adjusted to provide suitable illumination for the high-speed camera 1.

[0047] Working principle:

[0048] The DC motor 12 rotates at high speed, and the coupling 14 transmits power to the eccentric shaft 22, causing the eccentric shaft 22 to rotate at high speed. Under the transmission of the deep groove ball bearing 16, one end of the transmission rod 17 connected to the eccentric shaft 22 performs eccentric motion, while the other end performs linear reciprocating motion under the geometric constraints of the slider 18 and the guide rail 20. At the same time, the sample stage 19 is connected to the slider 18, and therefore performs linear reciprocating motion in the field of view of the long-distance objective lens 4. By adjusting the support 10 of the light source 9, the light source 9 is adjusted to an appropriate height and position. By appropriately changing the intensity of the light source 9, suitable illumination intensity is provided for the high-speed camera 1. The long-distance objective lens 4 is adjusted to a suitable position by the focusing device 15, and image signals are collected by the high-speed camera 1 to analyze the cell motility state.

[0049] In summary, this vibrating cell transfection microscopy observation platform has a vibration module and an observation module mounted on the base plate 11 of the material carrier module. The vibration module includes a DC motor 12 and a sample stage 19 connected to the DC motor 12 via an eccentric rotating shaft 22 and adapted to place the microfluidic chip 23. The motor shaft of the DC motor 12 rotates at high speed, driving the sample stage 19 to perform linear reciprocating motion via the eccentric rotating shaft 22. Simultaneously, the liquid contained in the microfluidic chip 23 oscillates with the movement of the sample stage 19, thereby causing the cells within the liquid to move. The observation module includes an observation mirror, a high-speed camera 1 mounted above the observation mirror, and a light source 9 mounted on the base plate 11 and located below the observation mirror. By adjusting the observation height, a suitable focal plane for imaging is found. The light source 9 illuminates the high-speed camera 1, and the high-speed camera 1 collects image signals from the observation mirror, thereby clearly observing the microchannel morphology of the liquid contained in the microfluidic chip 23 and the internal cell movement state and trajectory, thus achieving precise observation of the cell's movement under micro-mechanical behavior.

[0050] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A vibration-based cell transfection microscopic observation platform, characterized in that, include: The loading module includes a base plate (11) and a loading platform (6) mounted on the base plate (11); The vibration module includes a drive motor mounted on the base plate (11), a transmission assembly connected to the output end of the drive motor and located above the stage (6), and a sample stage (19) connected to the transmission assembly; the drive motor drives the sample stage (19) and the microfluidic chip (23) placed on it to perform linear reciprocating motion through the transmission assembly. The observation module includes an observation mirror located above the sample stage (19), a high-speed camera (1) disposed above the observation mirror, and a light source (9) mounted on the base plate (11) and located below the observation mirror; the sample stage (19) is located between the observation mirror and the light source (9), and the high-speed camera (1) is a CCD camera; the observation mirror, the light source (9), and the sample stage (19) are located on the same straight line; A guide rail (20) is fixed above the stage (6); the transmission assembly includes an eccentric shaft (22) coaxially connected to the drive motor via a coupling (14), and a transmission rod (17) connected to the eccentric shaft (22). One end of the transmission rod (17) moves eccentrically with the rotation of the eccentric shaft (22); the transmission assembly also includes a slider (18) connected to the other end of the transmission rod (17) and linearly slidably connected to the guide rail (20). The sample stage (19) is connected to the slider (18) and moves linearly back and forth with the slider. The transmission rod (17) has mounting holes at both ends; the eccentric shaft (22) is connected to one end of the transmission rod (17) through a first deep groove ball bearing located in the mounting hole; the slider (18) is fixed on a second deep groove ball bearing (16) located in another mounting hole; the sample stage (19) is Z-shaped; one end of the sample stage (19) is located between the observation mirror and the light source (9); and the other end of the sample stage (19) is connected to the other end of the transmission rod (17) through the second deep groove ball bearing (16).

2. The vibrating cell transfection microscopic observation platform according to claim 1, characterized in that, The platform (6) is mounted on the base plate (11) by a support rod (8). A fixing plate (5) is fixed on the upper surface of the platform (6), and the guide rail (20) is fixed on the fixing plate (5).

3. The vibrating cell transfection microscopic observation platform according to claim 2, characterized in that, The fixing plate (5) is fixedly installed on the stage (6) by screws (21). Both the stage (6) and the fixing plate (5) have through holes, and the two through holes are connected. The observation mirror, the light source (9), the sample stage (19) and the through holes are all located on the same straight line.

4. The vibrating cell transfection microscopic observation platform according to claim 2, characterized in that, A support frame (7) is fixed on the support rod (8), and the platform (6) is set on the support frame (7), and the platform (6) can slide along the X-axis and Y-axis in the horizontal direction.

5. The vibrating cell transfection microscopic observation platform according to claim 1, characterized in that, The sample stage (19) is provided with a first slot, and the first slot is provided with an elastic retaining spring suitable for limiting the second deep groove ball bearing (16); the eccentric rotating shaft (22) is provided with a second slot, and the second slot is provided with a stepped shaft suitable for limiting the first deep groove ball bearing.

6. The vibrating cell transfection microscopic observation platform according to claim 1, characterized in that, The observation module also includes a focusing device (15) mounted on the base plate (11); the observation lens includes a microscope tube (2) threadedly connected to the focusing device (15) and a long-distance objective lens (4) connected to the microscope tube (2); the long-distance objective lens (4) is located directly above the light source (9).

7. The vibrating cell transfection microscopic observation platform according to claim 6, characterized in that, A fixing ring (3) is slidably provided on the focusing device (15), and the microscope tube (2) is threadedly connected to the fixing ring (3); the long-distance objective lens (4) is connected to the long-distance objective lens (4) through an adapter ring; the light source (9) is set on the base plate (11) through a bracket (10), the illumination intensity of the light source (9) is adjustable, and the bracket (10) can be raised and lowered on the base plate (11).

8. The vibrating cell transfection microscopic observation platform according to claim 1, characterized in that, The drive motor is a DC motor (12).

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