Nucleic acid detection device

Abstract

The present invention belongs to the technical field of nucleic acid detection, and discloses a nucleic acid detection device, which includes: an outer shell, an upper cover, a container, and a puncturing piece. A reaction chamber for accommodating a reaction liquid and a placement chamber for placing a test paper are provided in the outer shell; the upper cover is provided on the outer shell to close the reaction chamber; the container is provided in the upper cover, and the upper cover is provided with a pressing hole for pressing the container, and the container has a holding chamber for accommodating a diluent. The puncturing piece is provided in the upper cover, and the puncturing piece is provided with a leak hole connected to the reaction chamber. The puncturing piece is used to puncture the bottom wall of the container, so that the diluent flows into the reaction chamber through the leak hole, so that the reaction product in the reaction chamber overflows into the placement chamber and contacts the test paper. In this way, the detection can be completed without opening the upper cover a second time during the nucleic acid detection process, which effectively reduces the possibility of aerosol contamination generated by the reaction product and improves the safety of the nucleic acid detection process.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to a nucleic acid detection device. Background Art

[0002] Nucleic acid testing refers to the technology of simulating DNA amplification in vivo in vitro to conduct corresponding detection. The main technologies currently used are PCR (polymerase chain reaction), LAMP (loop-mediated nucleic acid amplification) and RAA (recombinase-mediated isothermal nucleic acid amplification).

[0003] In existing technology, instrument-free in vitro nucleic acid testing typically utilizes a rapid test method by diluting the amplified product after a nucleic acid reaction and reacting it with a chromatographic test strip. The main process involves adding the nucleic acid generated after sample pretreatment to a reaction solution to form a test solution. This test solution is then injected into a test tube containing a reaction system. Nucleic acid amplification is performed under the conditions required for amplification, and the reaction product is contacted with a pre-placed chromatographic test strip to complete the test.

[0004] However, in the above detection process, in order to ensure that the reaction product is fully in contact with the chromatography test paper, the test tube needs to be opened twice to dilute the reaction product. Since the concentration of the reaction product is very high, it usually reaches 10 9 Copy / uL, the second opening of the test tube can easily cause aerosol contamination, resulting in reduced safety of the nucleic acid detection process and erroneous results. Summary of the Invention

[0005] The purpose of the present invention is to provide a nucleic acid detection device that solves the problem in the prior art that during the nucleic acid detection process, the test tube needs to be opened multiple times, which increases the risk of aerosol contamination, resulting in lower safety of the nucleic acid detection process and producing erroneous results.

[0006] To achieve this object, the present invention adopts the following technical solutions:

[0007] A nucleic acid detection device comprises: an outer shell, an upper cover, a container, and a puncturing member. The outer shell is provided with a reaction chamber for containing a reaction liquid and a placement chamber for placing a test paper; the upper cover is provided on the outer shell to close the reaction chamber; the container is provided in the upper cover, the upper cover is provided with a pressing hole for pressing the container, and the container has a storage chamber for containing a diluent. The puncturing member is provided in the upper cover, the puncturing member is provided with a leak hole connected to the reaction chamber, and the puncturing member is used to puncture the bottom wall of the container, so that the diluent flows into the reaction chamber through the leak hole, so that the reaction product in the reaction chamber is diluted and overflows into the placement chamber to contact the test paper.

[0008] Optionally, the nucleic acid detection device includes: a sample addition tube, which is arranged on the outer shell; an inner shell, which is arranged inside the outer shell, the reaction chamber is arranged on the inner shell and is connected to the sample addition tube, and an overflow groove is provided on the inner shell, and the overflow groove is located between the reaction chamber and the placement chamber.

[0009] Optionally, the nucleic acid detection device further includes: a mounting shell, disposed in the inner shell; a heating module, disposed on the mounting shell and located below the reaction chamber, wherein the heating module is used to heat the reaction liquid in the reaction chamber.

[0010] Optionally, the nucleic acid detection device further includes: a heat conducting portion, disposed between the heating module and the reaction chamber.

[0011] Optionally, the inner shell is movably connected to the outer shell, and the nucleic acid detection device further includes: an oscillator, which is arranged on the mounting shell, and the inner shell abuts against the top of the oscillator, and the oscillator is used to oscillate the reaction liquid in the reaction chamber.

[0012] Optionally, the nucleic acid detection device further includes: a power supply, which is disposed in the mounting shell, and the heating module and the oscillator are both electrically connected to the power supply.

[0013] Optionally, the accommodating member includes: a accommodating block, which is slidably connected to the upper cover, the accommodating cavity is arranged in the accommodating block, and an opening connected to the accommodating cavity is provided on the bottom wall of the accommodating block; and a sealing plate, which is arranged at the opening to close the accommodating cavity, and the puncturing member is used to puncture the sealing plate.

[0014] Optionally, the nucleic acid detection device further comprises: a plurality of clamping rods, which are arranged on the upper cover along the circumference of the upper cover, and the side surfaces of the clamping rods are in contact with the outer side walls of the container.

[0015] Optionally, the puncturing member includes: a fixed block fixed in the upper cover, the leakage hole is provided on the fixed block; and a ejector pin provided on the fixed block with the needle tip of the ejector pin facing the bottom wall of the accommodating member.

[0016] Optionally, the nucleic acid detection device further includes: an observation window, arranged on the side of the outer shell, the observation window being arranged opposite to the test paper; and a transparent sealing plate, arranged in the observation window, the transparent sealing plate being used to seal the observation window.

[0017] Beneficial effects of the present invention:

[0018] By arranging a reactor and a placement cavity in the outer shell, the upper cover is opened before testing, the test paper is placed, and then the reaction liquid is added to the reaction cavity. After the reaction liquid is added, the upper cover can be closed. After the reaction liquid is fully reacted in the reaction cavity, the container can be directly pressed through the pressing hole on the upper cover, so that the container moves toward the piercing part. The piercing part can pierce the bottom wall of the container and the diluent in the accommodating cavity flows out. The diluent flows into the reaction cavity through the leak hole. After dilution, the reaction product in the reaction cavity overflows the reaction cavity and enters the placement cavity. It can contact with the test paper so that the test paper produces a corresponding reaction and develops color, completing the nucleic acid test. In this way, the test can be completed without opening the upper cover a second time during the nucleic acid test process, effectively reducing the problem of aerosol pollution generated by the reaction product and improving the safety during the nucleic acid test process. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 Shown is a schematic structural diagram of a nucleic acid detection device in some embodiments of the present invention.

[0020] Figure 2 Shown are cross-sectional views of nucleic acid detection devices in some embodiments of the present invention.

[0021] Figure 3 Shown is a partially enlarged view of a nucleic acid detection device in some embodiments of the present invention.

[0022] Figure 4 Shown is a schematic flow chart of the nucleic acid detection method in some embodiments of the present invention.

[0023] In the picture:

[0024] 100, outer shell; 101, reaction chamber; 102, placement chamber; 110, sample tube; 120, observation window; 130, transparent sealing plate; 200, upper cover; 201, pressing hole; 210, connecting pipe; 220, holding rod; 300, accommodating part; 301, accommodating chamber; 310, accommodating block; 320, sealing plate; 400, piercing part; 401, leak hole; 410, fixing block; 420, ejector pin; 500, inner shell; 501, insertion rod; 510, abutment block; 600, mounting shell; 601, jack; 610, mounting chamber; 620, heating module; 630, heat conducting part; 640, oscillator; 700, power supply; 800, sealing gasket; 900, sealing ring. DETAILED DESCRIPTION

[0025] The present invention will be further described in detail below with reference to the accompanying drawings and examples. It will be understood that the specific embodiments described herein are intended only to illustrate the present invention and are not intended to limit the present invention. It should also be noted that, for ease of description, the accompanying drawings only illustrate portions relevant to the present invention, not all structures.

[0026] In the description of the present invention, unless otherwise expressly specified or limited, the terms "connected," "connected," and "fixed" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integration; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention in specific circumstances.

[0027] In the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Furthermore, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0028] In the description of this embodiment, the terms "upper," "lower," "right," and other orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely for ease of description and simplified operation. They do not indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on the present invention. Furthermore, the terms "first" and "second" are used solely for descriptive purposes and have no special meanings.

[0029] The present invention provides a nucleic acid detection device.

[0030] Figure 1 Shown is a schematic structural diagram of a nucleic acid detection device in some embodiments of the present invention. Figure 2 Shown are cross-sectional views of nucleic acid detection devices in some embodiments of the present invention. Figure 3 The figure shows a partial enlarged view of the nucleic acid detection device in some embodiments of the present invention. Figure 1 、 Figure 2 and Figure 3As shown. The nucleic acid detection device includes: an outer shell 100, an upper cover 200, a container 300 and a piercing member 400. A reaction chamber 101 for accommodating a reaction liquid and a placement chamber 102 for placing a test paper are provided in the outer shell 100. The upper cover 200 is provided on the outer shell 100 to close the reaction chamber 101. The container 300 is provided in the upper cover 200. The upper cover 200 is provided with a pressing hole 201 for pressing the container 300. The container 300 has a storage chamber 301 for accommodating a diluent. The piercing member 400 is provided in the upper cover 200. The piercing member 400 is provided with a leak hole 401 connected to the reaction chamber 101. The piercing member 400 is used to pierce the bottom wall of the container 300, so that the diluent flows into the reaction chamber 101 through the leak hole 401, so that the reaction product in the reaction chamber 101 overflows into the placement chamber 102 after dilution and contacts the test paper.

[0031] Specifically, the outer shell 100 can be in the shape of a cube, such as a cuboid or a cylinder, and its internal structure can be set in only one group, or two groups can be set symmetrically. When two groups are set, two reaction chambers 101 and two placement chambers 102 are set. A pipe opening connected to the corresponding reaction chamber 101 can be set on the outer shell 100, and the upper cover 200 can close the two pipe openings at the same time. Two pressing holes 201, two receiving parts 300 and two piercing parts 400 are correspondingly set in the upper cover 200, so that nucleic acid detection can be performed on two reaction products at the same time. It should be understood that the corresponding structure can also be set in three or more groups, and the shape change of the outer shell 100 can be adjusted according to the specific number of groups set to meet the needs of nucleic acid detection.

[0032] The shape of the upper cover 200 can be designed based on the shape of the outer shell 100, and the two can be interlocked to facilitate assembly and disassembly. In this embodiment of the present invention, a rod-shaped protrusion is provided on the inner side wall of the upper cover 200, and a notch is provided on the outer shell 100 for the upper cover 200 to be inserted. The rod-shaped protrusion abuts the side wall of the notch to achieve a fixed connection between the upper cover 200 and the outer shell 100.

[0033] The interior of the upper cover 200 can be hollow, that is, the upper cover 200 as a whole is in the shape of an empty shell with an open bottom. The connecting tube 210 is fixed inside the upper cover 200. The inner diameter of the connecting tube 210 is larger than the outer diameter of the pipe mouth, so that the connecting tube 210 can be sleeved with the pipe mouth. The top of the connecting tube 210 is connected to the pressing hole 201. The puncturing member 400 is set in the connecting tube 210, and the bottom wall of the puncturing member 400 abuts the end face of the pipe mouth, and the leakage hole 401 is connected to the pipe mouth. The container 300 can be placed in the connecting tube 210 in a cylindrical shape. The outer wall of the container 300 fits with the pipe wall of the connecting tube 210 to limit the movement of the container 300. The top of the container 300 extends into the pressing hole 201, and the top wall of the container 300 can be flush with the outer top wall of the upper cover 200.

[0034] When performing nucleic acid testing, the upper cover 200 is first opened and a reaction solution is added to the reaction chamber 101 through the nozzle. The reaction solution is formed by collecting a swab sample from the subject, extracting nucleic acid, and then adding it to a buffer solution. After the reaction solution is added, the upper cover 200 is closed, so that the connecting tube 210 is placed outside the nozzle, and the leak hole 401 is connected to the nozzle. Because the reaction chamber 101 is pre-installed with reaction reagents that can react with the reaction liquid, after the reaction liquid has fully reacted in the reaction chamber 101, the holding part 300 can be directly pressed through the pressing hole 201 on the upper cover 200, so that the holding part 300 moves toward the piercing part 400 in the connecting tube 210. The piercing part 400 can pierce the bottom wall of the holding part 300 and allow the diluent in the holding chamber 301 to flow out. The diluent flows into the reaction chamber 101 through the leakage hole 401. After being diluted, the reaction product in the reaction chamber 101 will overflow into the placement chamber 102 and contact the test paper, causing the test paper to produce a corresponding reaction and develop color, thereby completing the nucleic acid test.

[0035] In this way, the detection can be completed without opening the upper cover 200 a second time during the nucleic acid detection process, which effectively reduces the possibility of aerosol contamination caused by the reaction products and improves the safety of the nucleic acid detection process. In addition, the hollow setting of the upper cover 200 can also effectively reduce the weight of the entire device, making the device more convenient to carry. The upper cover 200 and the outer shell 100 form an interference fit through the rod-shaped protrusion, so that the connection strength between the upper cover 200 and the outer shell 100 is relatively high. When the outer shell 100 is discarded after the nucleic acid test is completed, the upper cover 200 is not easy to separate from the outer shell 100, thereby effectively improving its overall safety.

[0036] Reference Figure 3 As shown, in some embodiments of the present invention, the nucleic acid detection device includes a sample loading tube 110 and an inner housing 500. The sample loading tube 110 is disposed on the outer housing 100. The inner housing 500 is disposed within the outer housing 100, and the reaction chamber 101 is disposed in the inner housing 500 and communicates with the sample loading tube 110. The inner housing 500 is provided with an overflow groove, which is located between the reaction chamber 101 and the placement chamber 102.

[0037] Specifically, the sample tube 110 is fixed to the top wall of the outer shell 100 and extends vertically. Its upper end serves as the aforementioned nozzle and is inserted into the connecting tube 210, while its lower end communicates with the interior of the outer shell 100. The inner shell 500 is convex in shape, with its top wall concave inward to form the aforementioned reaction chamber 101. The top wall of the inner shell 500 is inclined toward the reaction chamber 101 to reduce the possibility of the reaction liquid flowing out of the reaction chamber 101 during filling.

[0038] Two reaction holes can be provided on the bottom wall of the reaction chamber 101 to accommodate the reaction liquid. The two reaction holes are spaced apart from each other, and the bottom wall of the reaction chamber 101 is in the shape of an inverted "bottom wall" and is inclined toward the two reaction holes. Two overflow grooves are also provided, and each overflow groove is spaced apart from the corresponding reaction hole. The overflow groove is provided near the placement chamber 102, and flow channels can be formed on the surface of the outer shell 100, respectively communicating with the overflow groove and the placement chamber 102. The two flow channels can converge into the same opening communicating with the placement chamber 102. It should be understood that the depth of the reaction hole and the overflow groove can be designed according to actual application requirements, and the present invention is not limited thereto.

[0039] After the reaction liquid is injected into the reaction chamber 101, the reaction liquid will first flow into the reaction hole. If there is a lot of reaction liquid, it will overflow the reaction hole and enter the overflow tank, so that the nucleic acid detection device can accommodate sufficient reaction liquid. At the same time, it ensures that the reaction liquid will not enter the placement chamber 102 and contact the test paper before it is fully reacted, thereby ensuring the accuracy of the nucleic acid detection results.

[0040] Reference Figure 2 and Figure 3 As shown, in some embodiments of the present invention, the nucleic acid detection device further includes a mounting shell 600 and a heating module 620. The mounting shell 600 is disposed within the inner housing 500. The heating module 620 is disposed on the mounting shell 600 and is located below the reaction chamber 101. The heating module 620 is used to heat the reaction solution in the reaction chamber 101.

[0041] Specifically, the inner shell 500 is hollow, and an insertion rod 501 is provided on the inner surface of the inner shell 500. A socket 601 for inserting the insertion rod 501 is provided on the top wall of the mounting shell 600 to connect the mounting shell 600 to the inner shell 500. The top wall of the mounting shell 600 is concave to form a mounting cavity 610, and the heating module 620 is provided on the bottom wall of the mounting cavity 610, and the mounting cavity 610 is located directly below the reaction hole. The heating module 620 can be an electric heating wire, which is coiled on the bottom wall of the mounting cavity 610. It should be understood that the heating module 620 can also be other structures, such as a heating rod, etc., and the present invention is not limited thereto.

[0042] After the reaction liquid is added to the reaction hole, the heating module 620 can be started to heat the inner shell 500, so that the heat will be transferred to the reaction liquid through the inner shell 500, so that the reaction liquid can be heated to the most suitable reaction temperature range, such as 37°C, which is most suitable and maintained for about 5-15 minutes to improve the reaction efficiency of the reaction liquid.

[0043] Reference Figure 2 and Figure 3As shown, in some embodiments of the present invention, the nucleic acid detection device further includes a heat conducting portion 630. The heat conducting portion 630 is arranged between the heating module 620 and the reaction chamber 101. Specifically, the heat conducting portion 630 is arranged in the installation cavity 610 and can be made of aluminum alloy material. The heat conducting portion 630 can be disc-shaped or cubic-shaped, and its bottom wall can be in close contact with the heating module 620, and its top wall is in close contact with the inner wall of the inner shell 500. By providing the heat conducting portion 630, the interval between the heating module 620 and the reaction hole is effectively reduced. After the heating module 620 is operated, the heat will first be transferred to the heat conducting portion 630, and then transferred to the reaction hole, thereby improving the efficiency of heat transfer and being able to quickly heat the reaction solution.

[0044] Reference Figure 2 and Figure 3 As shown, in some embodiments of the present invention, the inner housing 500 is movably connected to the outer housing 100, and the nucleic acid detection device further includes an oscillator 640. The oscillator 640 is disposed on the mounting shell 600, and the inner housing 500 abuts against the top of the oscillator 640. The oscillator 640 is used to oscillate the reaction solution in the reaction chamber 101.

[0045] Specifically, the oscillator 640 is disposed in the mounting cavity 610, and a slot is provided in the middle of the heat conducting portion 630, and the oscillator 640 is disposed in the slot. The inner sidewall of the inner housing 500 abuts against the top wall of the oscillator 640. When the reaction liquid is reacting, the oscillator 640 can be started and maintained for about 5-15 minutes. The oscillator 640 can generate vibrations and transmit the vibrations to the reaction chamber 101 through the inner sidewall of the inner housing 500, so that the reaction liquid in the reaction chamber 101 vibrates, thereby further improving the reaction efficiency of the reaction liquid.

[0046] Reference Figure 2 and Figure 3 As shown, in some embodiments of the present invention, the nucleic acid detection device also includes a power supply 700. The power supply 700 is arranged in the mounting shell 600, and the heating module 620 and the oscillator 640 are both electrically connected to the power supply 700. Specifically, a corresponding battery box is provided in the mounting shell 600, and the battery box is equipped with corresponding batteries as the power supply 700. It should be understood that the power supply 700 can also adopt other structures, as long as it can provide power to the heating module 620 and the oscillator 640, and the present invention is not limited thereto. A corresponding switch can also be provided on the outer shell 100 to adjust the start or shut down of the power supply 700. When performing nucleic acid detection, the power supply 700 is started by using the switch, so that the corresponding power can be provided by the built-in power supply 700 to increase the reaction speed of the reaction liquid, so that the nucleic acid detection device can be used in various environments that do not have power supply conditions, effectively improving the adaptability of the nucleic acid detection device.

[0047] Reference Figure 2 and Figure 3 As shown, in some embodiments of the present invention, the container 300 includes a container block 310 and a sealing plate 320. The container block 310 is slidably connected to the upper cover 200. The container cavity 301 is disposed within the container block 310. The bottom wall of the container block 310 is provided with an opening communicating with the container cavity 301. The sealing plate 320 is disposed at the opening to seal the container cavity 301, and the piercing member 400 is used to pierce the sealing plate 320.

[0048] Specifically, the accommodating block 310 is cylindrical, and its outer diameter can be equal to the inner diameter of the connecting tube 210, so that the outer wall of the accommodating block 310 fits with the tube wall of the connecting tube 210. The interior of the accommodating block 310 is hollow as the accommodating cavity 301, and its bottom wall is open. The sealing plate 320 can be fixed on the bottom wall of the accommodating block 310 by bonding or other means to close the opening. The sealing plate 320 can be made of aluminum-plastic material, which has a certain hardness and can carry the diluent in the accommodating cavity 301. At the same time, its material is relatively brittle and can be pierced by the piercing member 400. It should be understood that the sealing plate 320 can also be made of other materials, such as a sealing film, etc., which is not limited by the present invention.

[0049] After the reaction liquid completes the reaction, the holding block 310 can be squeezed through the pressing hole 201. The holding block 310 drives the sealing plate 320 to approach the piercing member 400, so that the piercing member 400 pierces the sealing plate 320. The diluent in the holding chamber 301 can flow out smoothly and flow into the reaction hole through the leakage hole 401. The reaction liquid in the reaction hole can overflow into the placement chamber 102. In this way, the reaction liquid can be brought into contact with the test paper without opening the upper cover 200.

[0050] In some embodiments of the present invention, the nucleic acid detection device further includes a latch. The latch is inserted into the upper cover 200, and one end of the latch is inserted into the receiving block 310. Specifically, a socket is provided on the upper cover 200, and a through hole connected to the socket is provided on the receiving block 310. One end of the latch is inserted into the socket and the through hole in turn, and a pull block can be provided at the other end of the latch. The cross-sectional area of ​​the pull block is larger than the cross-sectional area of ​​the latch to facilitate pulling out the latch. The outer wall of the upper cover 200 can be concave to accommodate the pull block to prevent the pull block from protruding from the outside of the upper cover 200.

[0051] Reference Figure 1 and Figure 3As shown, in some embodiments of the present invention, the nucleic acid detection device further includes a sealing gasket 800 and a sealing ring 900. The sealing gasket 800 is bonded to the top wall of the upper cover 200 and covers the pressing hole 201. The sealing gasket 800 can close the gap between the accommodating block 310 and the pressing hole 201, thereby improving the overall sealing. At the same time, before pressing the accommodating block 310, the sealing gasket 800 can be torn off first to indicate that the nucleic acid detection device has been used, so that the operator can handle it in time. The sealing ring 900 is sleeved on the outer side wall of the accommodating block 310 and abuts against the inner wall of the connecting tube 210 to achieve a sealed connection between the accommodating block 310 and the connecting tube 210.

[0052] Reference Figure 3 As shown, in some embodiments of the present invention, the nucleic acid detection device includes a plurality of clamping rods 220. The plurality of clamping rods 220 are arranged on the upper cover 200 along the circumference of the upper cover 200, with the side surfaces of the clamping rods 220 abutting against the outer wall of the container 300. Specifically, the clamping rods 220 are fixed to the inner wall of the connecting tube 210. The clamping rods 220 can be rectangular or circular rods and extend in the vertical direction. The side surfaces of the clamping rods 220 abut against the outer wall of the container block 310. The plurality of clamping rods 220 are arranged in a ring at intervals, so that the container block 310 is evenly clamped within the connecting tube 210, thereby limiting the movement of the container block 310. At the same time, the clamping rods 220 can also reduce the contact area between the container block 310 and the connecting tube 210, thereby reducing the friction experienced by the container block 310 during movement, thereby facilitating the sliding of the container block 310 within the connecting tube 210.

[0053] Reference Figure 3 As shown, in some embodiments of the present invention, the puncturing member 400 includes a fixing block 410 and a ejector pin 420. The fixing block 410 is fixed within the upper cover 200, and the leakage hole 401 is provided on the fixing block 410. The ejector pin 420 is provided on the fixing block 410, with the tip of the ejector pin 420 facing the bottom wall of the container 300.

[0054] Specifically, the fixed block 410 is disc-shaped and fixed to the inner wall of the connecting tube 210. A slot is provided on the bottom wall of the fixed block 410, into which the end wall of the tube mouth of the sample tube 110 is inserted, so that the leak hole 401 can be connected to the sample tube 110. The ejector pin 420 is located in the middle of the fixed block 410 and can be in the shape of a triangular pyramid, with its tip facing the sealing plate 320. It should be understood that the ejector pin 420 can also be conical or have other structures with a pointed top, and the present invention is not limited thereto.

[0055] When the receiving block 310 moves downward, the sealing plate 320 and the tip of the ejector pin 420 squeeze each other, and the ejector pin 420 can pierce the sealing plate 320, allowing the diluent in the receiving chamber 301 to flow out and into the reaction hole through the leak hole 401, so as to smoothly mix the diluent with the reaction product of the completed reaction and overflow to contact the test paper. After standing for 5-15 minutes, the result can be observed by the color change of the test paper.

[0056] In some embodiments of the present invention, the nucleic acid detection device further includes an observation window 120 and a transparent sealing plate 130. Observation window 120 is disposed on the side of the housing, opposite the test paper. Transparent sealing plate 130 is disposed within observation window 120 and is used to seal observation window 120.

[0057] Specifically, the outer shell 100 is provided with an opening communicating with the placement chamber 102 as an observation window 120, and a transparent sealing plate 130 is provided in the observation window 120 to close the observation window 120. The transparent sealing plate 130 can be a glass plate, which corresponds to the middle of the test paper in the placement chamber 102. It should be understood that the entire outer shell 100 can be made of a transparent material to facilitate the reaction of the reaction liquid and the liquid level of the reaction liquid in the placement chamber 102, thereby ensuring that the diluted reaction product can fully contact the test paper. The transparent sealing plate 130 can be a long strip-shaped plate structure, so that its cross-sectional area is larger for more convenient installation, and it can also better seal the observation window 120.

[0058] The present invention also provides a nucleic acid detection method, which is applied to the nucleic acid detection device in the above embodiment. Figure 4 FIG2 is a flow chart of a nucleic acid detection method in some embodiments of the present invention. Figure 4 As shown, the method includes:

[0059] Step S100: Place the nucleic acid detection device, open the upper cover 200, inject the reaction solution into the reactor through the sample injection tube 110, and then use the upper cover 200 to seal the outer shell 100.

[0060] Before use, the nucleic acid detection device has reaction reagents added to the reaction well in advance. During nucleic acid testing, the swab sample of the tester is first collected and the nucleic acid is extracted, and then mixed with the buffer solution to form a reaction liquid. Then, the upper cover 200 is opened and the sample is added to the reaction well through the sample tube 110 to contact the reaction reagent.

[0061] Step S200 : Turn on the power supply 700 , start the heating module 620 and the oscillator 640 to heat and oscillate the reaction reagents and the reaction solution.

[0062] After the reaction liquid and the reaction reagent are mixed, the heating module 620 can be started using the built-in power supply 700. The heat of the heating module 620 is transferred to the reaction chamber 101 through the heat conducting portion 630 to maintain the temperature in the reaction chamber 101 within a suitable range, for example, the temperature is maintained at 37°C. At the same time, the oscillator 640 is started to oscillate the inner shell 500 to ensure that the reaction liquid and the reaction reagent are fully mixed.

[0063] Step S300: After the power supply 700 is started for a predetermined time, the container 300 is pressed and the bottom wall of the container 300 is pierced by the piercing member 400, so that the diluent flows into the reaction chamber 101. The fully reacted reaction product is diluted and overflows into the placement chamber 102 to contact the test paper.

[0064] The preset time refers to the pre-set operating time of the heating module 620 and the oscillator 640. The preset time can be set according to the power of the power supply 700, the heating module 620, and the oscillator 640, for example, between 5 and 15 minutes, or other time. When the power supply 700 is on for more than the preset time, it is assumed that the reaction liquid has fully reacted. At this time, by pressing the container 300 to move the container 300 downward, and using the piercing member 400 to pierce the bottom wall of the container 300, the diluent will flow into the reaction chamber 101 through the leak hole 401, so that the reaction product can overflow into the placement chamber 102 after being diluted, and the reaction product can fully contact the test paper.

[0065] After the reaction product is fully in contact with the test paper, the outer shell 100 is left to stand for a certain period of time, such as 5-15 minutes, and the color development of the test paper can be observed between the transparent sealing plate 130 and the observation window 120.

[0066] Through the above-mentioned steps S100, S200 and S300, during the nucleic acid detection process, the upper cover 200 only needs to be opened when the reaction liquid is added. The reaction and dilution processes of the reaction liquid do not require the upper cover 200 to be opened a second time, making the nucleic acid detection process safer and more reliable, thereby improving the safety of the nucleic acid detection process.

[0067] Obviously, the above embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention and are not intended to limit the embodiments of the present invention. A person skilled in the art would be able to make various obvious changes, readjustments, and substitutions without departing from the scope of protection of the present invention. It is not necessary and impossible to enumerate all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the claims of the present invention.

Claims

1. A nucleic acid detection device, characterized in that: The nucleic acid detection device comprises: An outer shell (100), wherein a reaction chamber (101) for accommodating a reaction liquid and a placement chamber (102) for placing a test paper are provided in the outer shell (100); an upper cover (200) disposed on the outer shell (100) to seal the reaction chamber (101), wherein a connecting pipe (210) is fixed inside the upper cover (200); A container (300) is disposed in the connecting pipe (210), the upper cover (200) is provided with a pressing hole (201) for pressing the container (300), and the container (300) has a receiving cavity (301) for receiving a diluent; and a puncturing member (400) disposed in the upper cover (200), the puncturing member (400) being provided with a leaking hole (401) communicating with the reaction chamber (101), the puncturing member (400) being used to puncture the bottom wall of the container (300), so that the diluent flows into the reaction chamber (101) through the leaking hole (401), so that the reaction product in the reaction chamber (101) overflows into the placement chamber (102) and contacts the test paper; A sample adding tube (110) is provided on the outer shell (100) and inserted into the connecting tube (210); An inner shell (500) is arranged in the outer shell (100), the reaction chamber (101) is arranged in the inner shell (500) and is connected to the sample addition tube (110), and an overflow groove is provided on the inner shell (500), and the overflow groove is located between the reaction chamber (101) and the placement chamber (102); A mounting shell (600) is disposed inside the inner shell (500); A heating module (620) is provided on the mounting shell (600) and is located below the reaction chamber (101). The heating module (620) is used to heat the reaction liquid in the reaction chamber (101).

2. The nucleic acid detection device according to claim 1, characterized in that The nucleic acid detection device further includes: A heat-conducting component (630) is arranged between the heating module (620) and the reaction chamber (101).

3. The nucleic acid detection device according to claim 1, characterized in that The inner housing (500) is movably connected to the outer housing (100), and the nucleic acid detection device further comprises: The oscillator (640) is arranged on the mounting shell (600), and the inner shell (500) abuts against the top of the oscillator (640). The oscillator (640) is used to oscillate the reaction liquid in the reaction chamber (101).

4. The nucleic acid detection device according to claim 3, characterized in that The nucleic acid detection device further includes: The power supply (700) is arranged in the installation shell (600), and the heating module (620) and the oscillator (640) are both electrically connected to the power supply (700).

5. The nucleic acid detection device according to any one of claims 1 to 4, characterized in that The container (300) comprises: a receiving block (310) slidably connected to the upper cover (200), the receiving cavity (301) being disposed within the receiving block (310), and an opening communicating with the receiving cavity (301) being disposed on the bottom wall of the receiving block (310); and A sealing plate (320) is provided at the opening for sealing the accommodating cavity (301), and the piercing member (400) is used for piercing the sealing plate (320).

6. The nucleic acid detection device according to any one of claims 1 to 4, characterized in that The nucleic acid detection device further includes: A plurality of holding rods (220) are arranged on the upper cover (200) along the circumference of the upper cover (200), and the side surfaces of the holding rods (220) are in contact with the outer side walls of the accommodating member (300).

7. The nucleic acid detection device according to claim 1, characterized in that The piercing member (400) comprises: A fixed block (410) is fixed in the upper cover (200), and the leakage hole (401) is provided on the fixed block (410); and A ejector pin (420) is arranged on the fixing block (410) and the tip of the ejector pin (420) faces the bottom wall of the container (300).

8. The nucleic acid detection device according to claim 1, characterized in that The nucleic acid detection device further includes: an observation window (120), arranged on a side surface of the outer shell (100), the observation window (120) being arranged opposite to the test paper; and A transparent sealing plate (130) is arranged in the observation window (120), and the transparent sealing plate (130) is used to seal the observation window (120).

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