Fully automatic immunization analyzer with periodic automatic calibration and automatic calibration method thereof

Abstract

The application discloses a periodically automatic calibration full-automatic immune analyzer and an automatic calibration method thereof, and relates to the technical field of automatic calibration, and aims at solving the problems of low work efficiency and high cost in the prior art. The periodically automatic calibration full-automatic immune analyzer comprises a liquid taking module, a feeding module and a detection module, and the liquid taking module and the feeding module are arranged on the outer periphery of the detection module. The detection module comprises a supporting table, an outer ring and an inner ring arranged above the supporting table, and the outer ring and the inner ring are rotationally connected with the supporting table. An optical assembly is arranged above the supporting table, the optical assembly is located on the outer periphery of the outer ring, and the optical assembly is wirelessly connected with a data processor. A calibration object is arranged at the top of the outer ring. According to the application, the optical module is automatically calibrated, and the automatic calibration is safe, reliable, stable, cost-saving, and free of manual operation, so that the work efficiency is greatly improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of in vitro diagnosis POCT. More particularly, the present application relates to a periodically automatic calibration fully automatic immune analyzer and an automatic calibration method thereof. BACKGROUND

[0002] In the field of in vitro diagnosis POCT, it is well known to use fully automatic immune analyzers with different structural forms to achieve accurate and rapid detection of samples. In the process of researching and realizing accurate and rapid detection of samples, the inventors found that the fully automatic immune analyzers in the prior art at least have the following problems:

[0003] The existing immunofluorescence analysis device has the following problems: first, the optical module in the existing instrument is composed of multiple precision components and carefully selected lenses, which plays a crucial role in data detection. As the use cycle of the instrument itself accumulates, the external environment encounters or changes, which may affect the optical module, resulting in inaccurate detection and analysis results; second, the degree of automation is low, and there are many manual assistance steps, which can easily contaminate the blood sample and greatly affect the analysis accuracy; finally, the low degree of automation also leads to complex connection between each step and auxiliary operation, which takes more time and greatly increases the diagnosis and treatment time, which is not conducive to the timely diagnosis and treatment of patients with serious illness.

[0004] Therefore, it is necessary to develop a periodically automatic calibration fully automatic immune analyzer and an automatic calibration method thereof to solve the above problems. SUMMARY

[0005] In view of the deficiencies in the prior art, the main purpose of the present application is to provide a periodically automatic calibration fully automatic immune analyzer, which periodically controls the calibration material to be located directly below the optical module by an outer ring driving module, automatically calibrates the optical module, is safe, reliable and stable, does not produce consumables, saves costs, and is fully automatic without manual operation, has high automation degree, greatly improves work efficiency, and has wide market application value.

[0006] Another purpose of the present application is to provide a periodically automatic calibration fully automatic immune analyzer, which has high degree of automation, does not require manual assistance in the whole process, reduces the probability of contamination of blood samples, greatly shortens the immune analysis time, is conducive to timely diagnosis and treatment of the patient's condition, and enables the patient to receive timely and effective treatment.

[0007] In order to achieve these objects and other advantages in accordance with the present application, a periodically automatic calibration fully automatic immune analyzer is provided, comprising: a liquid taking module, a feeding module and a detection module, the liquid taking module and the feeding module are both arranged on the outer periphery of the detection module.

[0008] The detection module comprises a support table;

[0009] An outer ring and an inner ring are arranged above the support table, and the outer ring and the inner ring are rotationally connected with the support table; and

[0010] A data processor;

[0011] An optical assembly is arranged above the support table, and the optical assembly is located at the outer periphery of the outer ring, and the optical assembly is wirelessly connected with the data processor; a calibration object is arranged at the top end of the outer ring, and the outer ring is driven to rotate by an outer ring driving module, and the outer ring driving module periodically drives the outer ring to rotate in a forward direction or a reverse direction, so as to control the calibration object to be located directly below the optical assembly.

[0012] Preferably, the optical assembly comprises a position fine adjuster arranged in a horizontal direction;

[0013] A bearing frame is drivingly connected with the power output end of the position fine adjuster; and

[0014] An optical module is fixedly installed on the surface of the bearing frame;

[0015] The position fine adjuster drives the optical module to reciprocate in a horizontal direction, so as to fine adjust the position of the optical module.

[0016] Preferably, the optical module comprises an internally hollow fixed frame; and

[0017] A laser emitter, a dichroic mirror, a first lens, a filter, a second lens, a pinhole diaphragm and a detector are arranged inside the fixed frame;

[0018] The first lens, the dichroic mirror, the filter, the second lens, the pinhole diaphragm and the detector are sequentially arranged in a vertical direction from bottom to top, and the first lens, the dichroic mirror, the filter, the second lens, the pinhole diaphragm and the detector are coaxially arranged in a vertical direction, the laser emitter and the dichroic mirror are located at the same height in a vertical direction, the dichroic mirror is arranged in an inclined manner, and the detector is wirelessly connected with the data processor.

[0019] Preferably, the outer ring comprises a calibration portion, a positioning groove is arranged at the top end of the calibration portion, the positioning groove is matched with the calibration object, and the calibration object is placed in the positioning groove.

[0020] Preferably, a fluorescent focusing groove is arranged inside the calibration portion, the fluorescent focusing groove is located directly below the positioning groove, the cross section of the fluorescent focusing groove is in a circular arc shape, and the surface of the fluorescent focusing groove is treated as a light surface.

[0021] Preferably, the inner ring is embedded in and arranged concentrically with the outer ring, at least two object tables are fixedly arranged on the outer ring, a test paper unloading station, a test paper box replacing station and a fluorescence analysis station are sequentially and equidistantly arranged on the support table along the circumferential direction of the inner ring, at least one test paper box is arranged on the inner ring, and the optical assembly is arranged at the fluorescence analysis station.

[0022] Preferably, the inner ring is embedded in and arranged concentrically with the outer ring, at least two object tables are fixedly arranged on the outer ring, a test paper unloading station, a test paper box replacing station and a fluorescence analysis station are sequentially and equidistantly arranged on the support table along the circumferential direction of the inner ring, at least one test paper box is arranged on the inner ring, and the optical assembly is arranged at the fluorescence analysis station.

[0023] Preferably, the inner ring is embedded in and arranged concentrically with the outer ring, at least two object tables are fixedly arranged on the outer ring, a test paper unloading station, a test paper box replacing station and a fluorescence analysis station are sequentially and equidistantly arranged on the support table along the circumferential direction of the inner ring, at least one test paper box is arranged on the inner ring, and the optical assembly is arranged at the fluorescence analysis station.

[0024] Preferably, the inner ring is embedded in and arranged concentrically with the outer ring, at least two object tables are fixedly arranged on the outer ring, a test paper unloading station, a test paper box replacing station and a fluorescence analysis station are sequentially and equidistantly arranged on the support table along the circumferential direction of the inner ring, at least one test paper box is arranged on the inner ring, and the optical assembly is arranged at the fluorescence analysis station.

[0025] To achieve the above objects and other advantages, there is also provided an automatic calibration method of the periodic automatic calibration full-automatic immune analyzer according to the present application, comprising the following steps:

[0026] S1, selecting a calibration substance;

[0027] S2, establishing a database of the calibration substance and the optical module;

[0028] S3, periodically and automatically collecting data information of the optical module and the calibration substance, and uploading the data information to the database for analysis and comparison;

[0029] S4, correcting the set parameters of the optical module by using a parameter correction table;

[0030] S5, verifying the correction effect.

[0031] One of the above technical solutions has the following advantages or beneficial effects: the outer ring driving module periodically controls the calibration substance to be located directly below the optical module, the optical module is automatically calibrated, it is safe, reliable and stable, no consumables are generated, costs are saved, manual operation is not required, calibration is completed automatically, the degree of automation is high, work efficiency is greatly improved, and the method has wide market application value.

[0032] Another technical solution in the above technical solution has the following advantages or beneficial effects: it has a high degree of automation, the whole process does not need manual assistance, reduces the probability of blood sample contamination, greatly shortens the immune analysis time, is beneficial to the timely diagnosis and treatment of the patient's condition, and enables the patient to receive timely and effective treatment.

[0033] Other advantages, objects and features of the present application will be apparent from the following description, and will be understood by persons skilled in the art. BRIEF DESCRIPTION OF DRAWINGS

[0034] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings of the embodiments will be briefly introduced below, and obviously, the drawings in the following description only relate to some embodiments of the present application, but not limit the present application, wherein:

[0035] Figure 1 A three-dimensional structural view of the periodic automatic calibration full-automatic immune analyzer according to one embodiment of the present application;

[0036] Figure 2 A three-dimensional structural view of the detection module in the periodic automatic calibration full-automatic immune analyzer according to one embodiment of the present application;

[0037] Figure 3 A top view of the detection module in the periodic automatic calibration full-automatic immune analyzer according to one embodiment of the present application;

[0038] Figure 4 A three-dimensional structural view of the optical assembly in the periodic automatic calibration full-automatic immune analyzer according to one embodiment of the present application;

[0039] Figure 5 A partial exploded sectional view of the optical module and the outer ring in the periodic automatic calibration full-automatic immune analyzer according to one embodiment of the present application;

[0040] Figure 6 A partial sectional view of the optical module and the outer ring in the periodic automatic calibration full-automatic immune analyzer according to one embodiment of the present application;

[0041] Figure 7 A partial sectional view of the optical module and the outer ring in the periodic automatic calibration full-automatic immune analyzer according to one embodiment of the present application;

[0042] Figure 8 A front view of the test paper box and the test paper box seat in the periodic automatic calibration full-automatic immune analyzer according to one embodiment of the present application;

[0043] Figure 9 A three-dimensional structural view of a test paper box cooperating with a test paper box seat in a periodic automatic calibration full-automatic immune analyzer according to an embodiment of the present application;

[0044] Figure 10 A flow chart of an automatic calibration method of a periodic automatic calibration full-automatic immune analyzer according to an embodiment of the present application;

[0045] Figure 11 A fluorescence spectrum of a ruby in an automatic calibration method of a periodic automatic calibration full-automatic immune analyzer according to an embodiment of the present application;

[0046] Figure 12 A step chart of an automatic calibration method of a periodic automatic calibration full-automatic immune analyzer according to an embodiment of the present application. DETAILED DESCRIPTION

[0047] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0048] In the drawings, for the sake of clarity, the shapes and sizes can be exaggerated and the same reference numerals will be used throughout different drawings to designate the same or like components.

[0049] Unless otherwise defined, technical or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terms "first", "second", and similar terms do not denote any order, quantity, or importance, but are used to identify different components. Also, the terms "a" and "an" do not denote a limitation of quantity, but rather denote the presence of at least one of the referenced items. The terms "including" and / or "comprising", as well as other variants such as "include", "includes", "included", "including", "comprise", "comprising", "comprises", "comprised", and / or "comprised of", mean that the item(s) in the list are present, and do not preclude additional, unrecited item(s). The terms "upper", "lower", "left", "right", and the like, are used to denote relative positions only and thus, if the positions of the described objects are changed, the relative positions can also be changed accordingly.

[0050] In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, and the like are defined with respect to the configuration shown in the drawings, and in particular, "height" corresponds to the dimension from top to bottom, "width" corresponds to the dimension from left to right, and "depth" corresponds to the dimension from front to back, which are relative concepts and thus can change according to different positions and different use states, and therefore, these or other orientations should not be used to explain as restrictive terms.

[0051] Terms relating to attachment, coupling, and the like (for example, "connected" and "attached") refer to a relationship in which structures are fixed or attached to each other directly or indirectly through intermediate structures, and movable or rigid attachment, unless explicitly stated otherwise.

[0052] According to an embodiment of the present application, in combination Figures 1-9 As shown in the drawings, the periodic automatic calibration fully automatic immune analyzer comprises a liquid taking module 1, a feeding module 2, and a detection module 3, wherein the liquid taking module 1 and the feeding module 2 are arranged on the outer periphery of the detection module 3;

[0053] The detection module 3 comprises a support table 31;

[0054] An outer ring 32 and an inner ring 35 are arranged above the support table 31, and the outer ring 32 and the inner ring 35 are rotationally connected with the support table 31; and

[0055] A data processor;

[0056] An optical assembly 33 is arranged above the support table 31, the optical assembly 33 is located on the outer periphery of the outer ring 32, and the optical assembly 33 is wirelessly connected with the data processor; a calibration object 34 is arranged at the top end of the outer ring 32, the outer ring 32 is driven to rotate by an outer ring driving module, the outer ring driving module periodically drives the outer ring 32 to rotate in a forward direction or a reverse direction, so as to control the calibration object 34 to be located directly below the optical assembly 33.

[0057] In a preferred embodiment, the calibration object 34 is a ruby, and the ruby contains Cr elements, which can undergo electronic transition under the excitation of laser of a specific wavelength.

[0058] The data processor is a tablet computer.

[0059] Further, in combination Figure 4 The optical assembly 33 comprises a position fine adjuster 331 arranged in a horizontal direction.

[0060] A receiving frame 332 is in transmission connection with the power output end of the position fine tuner 331; and

[0061] An optical module 333 is fixedly installed on the surface of the receiving frame 332.

[0062] The position fine tuner 331 drives the optical module 333 to reciprocate in the horizontal direction, so as to fine tune the position of the optical module 333.

[0063] Further, in combination with Figure 5 The optical module 333 comprises: an internal hollow fixed frame 3331; and

[0064] A laser emitter 3332, a dichroic mirror 3333, a first lens 3334, a filter 3335, a second lens 3336, a pinhole diaphragm 3337 and a detector 3338 are arranged inside the fixed frame 3331.

[0065] The first lens 3334, the dichroic mirror 3333, the filter 3335, the second lens 3336, the pinhole diaphragm 3337 and the detector 3338 are arranged in the vertical direction from bottom to top, and are coaxially arranged in the vertical direction. The laser emitter 3331 and the dichroic mirror 3333 are located at the same height in the vertical direction. The dichroic mirror 3333 is arranged obliquely. The detector 3338 is wirelessly connected to the data processor.

[0066] Further, the outer ring 32 comprises a calibration part 321, and a positioning groove 3211 is formed at the top end of the calibration part 321. The positioning groove 3211 is matched with the calibration object 34, and the calibration object 34 is placed in the positioning groove 3211.

[0067] Further, a fluorescent focusing groove 3212 is formed inside the calibration part 321, and the fluorescent focusing groove 3212 is located directly below the positioning groove 3211. The cross section of the fluorescent focusing groove 3212 is in the shape of a circular arc, and the surface of the fluorescent focusing groove 3212 is treated as a light surface.

[0068] It can be understood that, in combination with Figure 6 and Figure 7 After the ruby is excited to emit fluorescence, the fluorescence is stereoscopic light emission. The traditional capturing method only collects the fluorescence on a single side of the ruby by a lens, and the collection effect is poor, and most of the fluorescence cannot be effectively collected.

[0069] The present application is provided with a fluorescent focusing groove 3212 directly below the positioning groove 3211 for placing the ruby, and the surface of the fluorescent focusing groove 3212 is polished, so that the fluorescent light emitted from the lower end surface of the ruby is refracted immediately after contacting the polished surface of the fluorescent focusing groove 3212, and the fluorescent light emitted from the upper end surface of the ruby is focused on a point, greatly reducing the dissipation of fluorescent light in other directions, and amplifying the fluorescent signal by increasing the collection surface area.

[0070] In a preferred embodiment of the present application, the optical assembly 33 further comprises an alarm device wirelessly connected to the data processor.

[0071] It can be understood that when the optical assembly 33 is automatically calibrated and identified multiple times, the data processor controls the alarm device to issue an alarm to remind the staff to perform manual calibration.

[0072] In summary, the outer ring driving module periodically drives the outer ring 32 to rotate in the forward or reverse direction to control the calibration object 34 to be located directly below the optical module 333, the laser emitter 3332 emits excitation light, which is refracted by the dichroic mirror 3333 and focused on the ruby through the first lens 3334, the ruby is excited to emit fluorescent light, the emitted fluorescent light is focused and collected by the fluorescent focusing groove 133 to the focal point of the first lens 3334, and then passes through the first lens 3334, the dichroic mirror 3333, the optical filter 3334, the second lens 3336, and the pinhole diaphragm 3337 to reach the detector 3338, the detector 3338 reads the data information of the fluorescent light and uploads the data to the data processor.

[0073] Further, in combination with Figure 2 and Figure 3 , the inner ring 35 is embedded in the outer ring 32 and arranged concentrically with the outer ring 32, at least two object tables 322 are fixedly arranged on the outer ring 32, a test paper unloading station 311, a test paper box replacing station 312, and a fluorescent analysis station 313 are sequentially and equidistantly arranged on the support table 31 along the circumferential direction of the inner ring 35, at least one test paper box 36 is arranged on the inner ring 35, and the optical assembly 33 is arranged at the fluorescent analysis station 313.

[0074] In a preferred embodiment, the outer periphery of the inner ring 35 is in sliding contact with the inner periphery of the outer ring 32, so that the inner ring 35 and the outer ring 32 can smoothly and stably rotate relative to each other.

[0075] Further, in combination with Figure 2 and Figure 3The inner ring 35 is fixedly installed with test paper box seats 38, and the lower half of the test paper box 36 is detachably inserted into the test paper box seat 38, and the number of the test paper box seats 38 corresponds to the number of the test paper boxes 36.

[0076] Further, in combination with Figure 8 and Figure 9 The root of the test paper box seat 38 is provided with test paper pushing-out ports 381 penetrating through the front and back sides thereof, and the bottom of the test paper box 36 is provided with a test paper self-falling groove 361 in communication with the test paper pushing-out ports 381.

[0077] Further, the front side of the test paper box seat 38 is provided with a left guide seat 382 and a right guide seat 383 respectively located on the left and right sides of the test paper pushing-out ports 381, and the left guide seat 382 and the right guide seat 383 are respectively provided with oppositely arranged left and right guide grooves 3821 and 3831, and the faces of the left and right guide grooves 3821 and 3831 are located on the same horizontal plane as the bottom face of the test paper self-falling groove 361, so that the test paper pushed out from the test paper self-falling groove 361 can be smoothly received and guided by the left and right guide grooves 3821 and 3831 into the test paper loading groove for subsequent drop operation.

[0078] In the preferred embodiment, three test paper boxes 36 are equidistantly spaced in the circumferential direction of the inner ring 35.

[0079] The inner ring 35 is driven to rotate by an inner ring driving module, and the support table 31 is further provided with a test paper pushing-out unit, and the inner ring 35 is provided with an inner ring rotation angle sensor 351 for sensing the rotation angle of the inner ring 35.

[0080] The side of each test paper box 36 is provided with a buffer solution plate 37.

[0081] The clockwise or counterclockwise rotation of the outer ring 32 and the inner ring 35 does not affect the specific implementation of the technical scheme in the present application, and here the counterclockwise rotation of the outer ring 32 and the inner ring 35 is taken as an example for detailed description.

[0082] The steps of immunofluorescence analysis are as follows:

[0083] First, the inner ring 35 is rotated so that one of the test paper boxes 36 thereon is rotated to the test paper unloading station 311, and the other two test paper boxes 36 on the inner ring 35 are located at the test paper box replacing station 312 and the fluorescence analysis station 313 respectively, at the same time, the outer ring driving module drives the outer ring 32 so that one of the test paper loading grooves thereon is opposite to the test paper pushing-out port 381, and the test paper pushing-out unit is pushed outward, so that the bottom test paper in the test paper box 36 on the test paper unloading station 311 is pushed into the test paper loading groove;

[0084] Then, the outer ring 32 is driven to rotate anticlockwise by one test strip carrier station, so that the next test strip carrier is aligned with the test strip pushing outlet 381, and the previous test strip carrier is rotated to the side of the buffer plate 37, at this time, the blood sample, the buffer and the fluorescently labeled antibody solution are sequentially dropped into the previous test strip by the mechanical hand;

[0085] Further, the outer ring 32 is continuously and intermittently rotated anticlockwise, so that the first test strip carrying the blood sample is rotated to the fluorescent analysis station 313 to receive the fluorescence analysis of the optical assembly 33, when the first test strip completes the analysis, the outer ring 32 continues to rotate anticlockwise to the waste disposal station, and the analyzed test strip is collected and recycled with the assistance of the mechanical hand, and the cycle is repeated until all the collected blood samples are analyzed.

[0086] In the above-mentioned immunofluorescence analysis step, when the test strip cartridge 36 at the test strip unloading station 311 is used up, the inner ring 35 is rotated by 120°, so that the test strip cartridge 36 at the fluorescent analysis station 313 is rotated to the test strip unloading station 311, the test strip cartridge 36 at the test strip cartridge replacement station 312 is rotated to the fluorescent analysis station 313, and the test strip cartridge 36 at the test strip unloading station 311 is rotated to the test strip cartridge replacement station 312, so that the replacement of the full / empty test strip cartridge 36 can be carried out at the test strip cartridge replacement station 312, greatly shortening the replacement time of the test strip cartridge 36 and improving the analysis efficiency.

[0087] In combination Figures 10-12 The present application also provides an automatic calibration method of the periodic automatic calibration full-automatic immunological analyzer, comprising the following steps:

[0088] S1, selecting a calibration substance;

[0089] S2, establishing a database of the calibration substance and the optical module;

[0090] S3, periodically and automatically collecting data information of the optical module and the calibration substance, and uploading to the database for analysis and comparison with the data in the database;

[0091] S4, correcting the set parameters of the optical module by using a parameter correction table;

[0092] S5, verifying the correction effect.

[0093] In the preferred embodiment, the calibration substance in step S1 is selected as a ruby.

[0094] It can be understood that the ruby contains Cr element, which can undergo electronic transition under the excitation of laser of a specific wavelength.

[0095] In combination Figure 11is the fluorescence spectrum of ruby under the irradiation of excitation light at different wavelengths.

[0096] The peak value of the curve is near 691.3 nm and changes with the wavelength of the excitation light, which can be used as a calibration object 13 for verification.

[0097] Further, the specific steps for establishing the database of the calibration object and the optical module in step S2 are as follows:

[0098] a1. Rotating the driving module to drive the outer ring 32 to rotate in the positive direction, so that the calibration object 34 is located directly below the optical module 333;

[0099] a2. Setting the parameter low value A, the parameter medium value B and the parameter high value C of the laser emitter 3332, and the laser emitter 3332 scans the calibration object 34 for 20 rounds respectively using the parameter low value A, the parameter medium value B and the parameter high value C;

[0100] a3. After the detector 3338 obtains the data information of the 20 rounds of scanning of the calibration object 34 by the laser emitter 3332 using the parameter low value A, the parameter medium value B and the parameter high value C respectively, the obtained data information is uploaded to the data processor for processing, so as to generate a database in the data processor.

[0101] In a preferred embodiment of the present application, the adjustable range of the parameters of the laser emitter 3332 is 0-110.

[0102] Setting the parameter low value A=1, the parameter medium value B=10 and the parameter high value C=100, and placing the ruby in the positioning groove 3211, the ruby is scanned for 20 rounds respectively using the parameter low value A=1, the parameter medium value B=10 and the parameter high value C=100, and the obtained data information is shown in the following table:

[0103]

[0104]

[0105] As can be seen from the data in the above table, the ruby is excited by multiple rounds of excitation light and emits stable and reliable fluorescence, which can be used as an effective basis for comparison. Meanwhile, the specific values of the parameter low value A, the parameter medium value B and the parameter high value C can be used as data for data analysis and comparison of the database.

[0106] Further, the specific steps for periodically and automatically collecting the data information of the optical module and the calibration object in step S3 and uploading the data information to the database for analysis and comparison with the data in the database are as follows:

[0107] b1. Rotating the driving module to drive the outer ring 32 to rotate in the positive direction, so that the calibration object 34 is located directly below the optical module 333;

[0108] b2, set the laser emitter 3332 with parameter low value A, parameter middle value B and parameter high value C, and the laser emitter 3332 scans the calibration object 34 with parameter low value A, parameter middle value B and parameter high value C respectively;

[0109] b3, the detector 3338 uploads the data information obtained by the laser emitter 3332 scanning the calibration object 34 with parameter low value A, parameter middle value B and parameter high value C respectively to the data processor and the database for analysis and comparison;

[0110] b4, if the data information collected with parameter low value A, parameter middle value B and parameter high value C is within the preset range of the data information in the database, the data processor records the collected data information and ends the verification; if the data information collected with parameter low value A, parameter middle value B and parameter high value C is not within the preset range of the data information in the database, parameter correction is entered.

[0111] In an embodiment of the present application, the parameters set by the laser emitter 3332 in the optical module 333 when leaving the factory are 3 groups, which are parameter low value A, parameter middle value B and parameter high value C, and the data and receiving range are A±10%, B±10% and C±10% respectively, which are qualified, and the rest are unqualified.

[0112] Further, the specific steps of correcting the set parameters of the optical module by using the parameter correction table in step S4 are as follows:

[0113] c1, if the data analysis and comparison exceeds the preset range, the corresponding parameter value is obtained from the parameter comparison table based on the current reading range, and the current parameter value of the laser emitter 3332 is modified to the obtained parameter value;

[0114] c2, after the initial parameter correction is completed, the laser emitter 3332 repeatedly scans the calibration object 34 with the modified parameter value for verification until the verification is completed.

[0115] In a preferred embodiment of the present application, if the parameter correction is still not completed after 3 times, the data processor controls the alarm to issue an alarm to remind the staff to perform manual calibration.

[0116] In an embodiment of the present application, the following parameter correction table can be used to read the data corresponding to the correction coefficient correction parameter.

[0117] Serial number Reading Correction factor 1 5.9 +0.2 2 5.8 +0.1 3 5.7 0 4 5.6 -0.1 5 5.5 -0.2

[0118] Further, the specific steps of verifying the correction effect in step S5 are as follows:

[0119] d1, select four automatic immune analyzers, marked as No. 1, No. 2, No. 3 and No. 4, No. 1 and No. 2 instruments are not automatically checked, No. 3 and No. 4 instruments are automatically checked every 50 days;

[0120] d2, set the laser emitter 3332 of No. 1 automatic immune analyzer, No. 2 automatic immune analyzer, No. 3 automatic immune analyzer and No. 4 automatic immune analyzer to the same parameter value, collect the data information of the optical module 333 of each automatic immune analyzer every 10 days, and record;

[0121] d3, compare and analyze the collected data information of the optical module 333 of each automatic immune analyzer.

[0122] In an embodiment of the application, the parameter value of the laser emitter 3332 of No. 1 automatic immune analyzer, No. 2 automatic immune analyzer, No. 3 automatic immune analyzer and No. 4 automatic immune analyzer is set to 10, the data information of the optical module 333 of each automatic immune analyzer is collected every 10 days, and recorded in the following table;

[0123] Days Machine No. 1 Machine No. 2 Machine No. 3 Machine No. 4 Remarks 10 10.02 10.11 10.06 10.03 20 10.12 10.22 10.05 10.09 30 10.13 10.22 10.16 10.14 40 10.24 10.33 10.18 10.25 50 10.35 10.35 10.26 10.38 Automatic check 60 10.45 10.36 10.03 10.02 70 10.46 10.46 10.09 10.12 80 10.45 10.42 10.12 10.17 90 10.52 10.49 10.19 10.24 100 10.55 10.52 10.22 10.29 Automatic check

[0124] In the above table, the data value detected by No. 1 automatic immune analyzer and No. 2 automatic immune analyzer increases with the increase of days, and the use cycle of the instrument itself accumulates, the external environment is affected or changed to affect the optical module, which causes the detection data to gradually increase, and the detection result error gradually increases, while No. 3 automatic immune analyzer and No. 4 automatic immune analyzer can restore to normal after automatic calibration, and the automatic calibration method is effective, safe, reliable and stable.

[0125] The number of devices and the scale of processing described herein are used to simplify the description of the application. The application, modification and change of the application to those skilled in the art are obvious.

[0126] Although the embodiments of the application have been disclosed as above, it is not limited to the application listed in the specification and the embodiments. It can be fully applied to various fields suitable for the application. Additional modifications can be easily realized by those skilled in the art. Therefore, the application is not limited to specific details and the figures shown and described herein without departing from the general concept defined by the claims and the equivalent scope.

Claims

1. A fully automated immunoassay analyzer with periodic automatic calibration, characterized in that, The utility model relates to a kind of automatic detection device for fluorescence analysis of test paper, including: Liquid taking module (1), feeding module (2) and detection module (3), the liquid taking module (1) and the feeding module (2) are both set to the outer periphery of the detection module (3); Wherein, the detection module (3) includes: support table (31); Outer ring (32) and inner ring (35) are provided on the support table (31), the outer ring (32) and the inner ring (35) are rotatably connected with the support table (31);And Data processor; The upper side of the support table (31) is provided with optical assembly (33), and the optical assembly (33) is located at the outer periphery of the outer ring (32), and the optical assembly (33) is wirelessly connected with the data processor;The top of the outer ring (32) is provided with a calibration object (34), and the outer ring (32) is driven to rotate by an outer ring driving module, and the outer ring driving module periodically drives the outer ring (32) to rotate in a forward direction or a reverse direction to control the calibration object (34) to be located directly below the optical assembly (33); Wherein, the calibration object (34) is ruby; The outer ring (32) includes a calibration part (321), and a positioning groove (3211) is formed in the top of the calibration part (321), the positioning groove (3211) is matched with the calibration object (34), and the calibration object (34) is placed in the positioning groove (3211); A fluorescent focusing groove (3212) is formed in the inside of the calibration part (321), and the fluorescent focusing groove (3212) is located directly below the positioning groove (3211), the cross section of the fluorescent focusing groove (3212) is in the shape of a circular arc, and the surface of the fluorescent focusing groove (3212) is treated as a light surface; The optical assembly (33) includes: a position fine adjuster (331) arranged in a horizontal direction; A receiving frame (332) is drivingly connected with the power output end of the position fine adjuster (331);And An optical module (333) is fixedly installed on the surface of the receiving frame (332); Wherein, the position fine adjuster (331) drives the optical module (333) to reciprocate in a horizontal direction to fine adjust the position of the optical module (333); An alarm is wirelessly connected with the data processor; The inner ring (35) is embedded in the outer ring (32) and is concentrically arranged with the outer ring (32), at least two object tables (322) are fixedly arranged on the outer ring (32), test paper unloading stations (311), test paper box replacement stations (312) and fluorescent analysis stations (313) are sequentially and equidistantly arranged on the support table (31) along the circumferential direction of the inner ring (35), at least one test paper box (36) is arranged on the inner ring (35), and the optical assembly (33) is arranged in the fluorescent analysis station (313).

2. The fully automated immunoassay analyzer with periodic self-calibration of claim 1, wherein, The optical module (333) includes: an internal hollow fixed frame (3331);And A laser emitter (3332), a dichroic mirror (3333), a first lens (3334), a filter (3335), a second lens (3336), a pinhole diaphragm (3337) and a detector (3338) are arranged inside the fixed frame (3331); The first lens (3334), the dichroic mirror (3333), the filter (3335), the second lens (3336), the pinhole diaphragm (3337) and the detector (3338) are arranged in a vertical direction from bottom to top, and are coaxially arranged in the vertical direction, the laser emitter (3332) and the dichroic mirror (3333) are located at the same height in the vertical direction, the dichroic mirror (3333) is arranged obliquely, and the detector (3338) is wirelessly connected to the data processor.

3. The fully automated immunoassay analyzer with periodic self-calibration of claim 1, wherein, The inner ring (35) is fixedly provided with a test paper box seat (38), and the lower half of the test paper box (36) is detachably inserted into the test paper box seat (38), and the number of the test paper box seat (38) corresponds to the number of the test paper box (36).

4. The fully automated immunoassay analyzer with periodic self-calibration of claim 3, wherein, The root of the test paper box seat (38) is provided with a test paper pushing outlet (381) penetrating through the front and back sides thereof, and the bottom of the test paper box (36) is provided with a test paper self-falling groove (361) in communication with the test paper pushing outlet (381).

5. The fully automated immunoassay analyzer with periodic self-calibration of claim 4, wherein, The front side of the test paper box seat (38) is provided with a left guide seat (382) and a right guide seat (383) located on the left and right sides of the test paper pushing outlet (381) respectively, and the left guide seat (382) and the right guide seat (383) are respectively provided with oppositely arranged left and right guide grooves (3821) and (3831), and the faces of the left and right guide grooves (3821) and (3831) are located on the same horizontal plane as the bottom face of the test paper self-falling groove (361).

6. A method for automatic calibration of a fully automatic immunoassay analyzer of the type periodically self-calibrating according to any one of claims 1 to 5, characterized in that, The method comprises the following steps: S1, selecting a calibration object; S2, establishing a database of the calibration object and the optical module; S3, periodically and automatically collecting data information of the optical module and the calibration object, and uploading to the database and the data in the database for analysis and comparison; S4, correcting the set parameters of the optical module by using a parameter correction table; S5, verifying the correction effect.

Images