An immunization method under ASF conditions

Abstract

The present invention discloses an immunization method under ASF conditions, belonging to the technical field of breeding, and comprising the following steps: Step 1, first group one-day-old piglets into groups A, B, C, and D; Step 2, five days before 35 days, 56 days, and 90 days, switch to a stress response adaptation breeding plan for breeding. This immunization method under ASF conditions can reduce the occurrence of stress responses by grouping piglets and simultaneously injecting multiple vaccines. At the same time, before vaccination, it can further gradually adapt to avoid stress responses during the vaccination process and reduce the excessive stress responses caused by weaning. During the subsequent observation process, it can prevent vaccination, reduce the number of piglets that are not adapted after vaccination, and appropriately control the breeding environment to avoid infections and diseases caused by the injection sites.

Description

Technical Field

[0001] The invention relates to the technical field of breeding, and in particular to an immunization method under non-swine fever conditions. Background Art

[0002] Farming refers to the cultivation and reproduction of animals and plants. Farming includes livestock farming, poultry farming, aquaculture and special breeding.

[0003] Classical swine fever live vaccine is used to prevent classical swine fever. It can be used alone or in combination with anti-classic swine fever serum for emergency prevention in pigs with classical swine fever. This vaccine is made by inoculating healthy rabbits or suckling rabbits with the lapinized attenuated strain of classical swine fever virus, harvesting the spleen and lymph nodes of infected rabbits, or the muscles and solid organs of suckling rabbits, grinding them into a homogenate, adding appropriate stabilizers, and freeze-vacuum drying.

[0004] Taking into account the existing farms, in order to ensure the control of swine fever, vaccination is often needed for control and prevention. In the subsequent breeding process, multiple vaccinations with different types of vaccines will increase the frequency of stress reactions, further increasing the breeding costs. It is also very easy to cause infection and other diseases due to punctures. There are uncontrollable factors in the vaccination of the same batch of vaccines, and large-scale vaccination is very likely to cause mass deaths or other problems. Summary of the invention

[0005] The invention provided by the present invention aims to provide an immunization method under non-swine fever conditions. Through the immunization method under non-swine fever conditions of the present invention, the immunization method under non-swine fever conditions can reduce the generation of stress reactions by grouping piglets and injecting multiple vaccines simultaneously. At the same time, before vaccination, further gradual adaptation can be achieved to avoid stress reactions during the vaccination process, and excessive stress reactions caused by weaning can be reduced. In the subsequent observation process, vaccination can be prevented, the number of inadaptations caused by the inoculated piglets can be reduced, and the breeding environment can be appropriately controlled to avoid infection and diseases caused by needle holes.

[0006] In order to achieve the above effects, the present invention provides the following technical solution: an immunization method under non-swine fever conditions, comprising the following steps:

[0007] Step 1: First, group the one-day-old piglets into groups A, B, C and D.

[0008] Step 2: On the 35th day, 56th day and five days before the 90th day, switch the breeding plan to adapt to the stress response.

[0009] Step 3. On the 35th day, select Group A for vaccination with Classical Chinese Fever vaccine and the original branch two-shot vaccine, record the vaccination environment, observe the stress response of Group A, optimize and control the vaccination environment, and complete the vaccination of Groups B, C and D with Classical Chinese Fever vaccine and the original branch two-shot vaccine.

[0010] Step 4. On the 56th day, after Group A is eliminated, select one group at random, record the vaccination environment, vaccinate with pseudorabies and foot-and-mouth disease vaccines, observe the stress response, optimize and control the vaccination environment, and complete the vaccination of the remaining three groups with pseudorabies and foot-and-mouth disease vaccines.

[0011] Step 5. On the 90th day, after excluding Group A and the 56th day priority vaccination group, choose one of the remaining two groups and vaccinate them with pseudorabies and foot-and-mouth disease vaccines, observe the stress response, optimize and control the vaccination environment, and complete the vaccination of the remaining three groups with pseudorabies and foot-and-mouth disease vaccines.

[0012] Step 6. Ten days after each vaccination, random sampling will be conducted to test antibody concentration and determine the effectiveness of the vaccination.

[0013] Furthermore, according to the operating procedures in step one, the one-day-old piglets need to be sampled in advance to determine whether they are infected with the virus to ensure healthy breeding.

[0014] Further, according to the operation steps in step 2, the stress response adaptation breeding program is divided into a pre-weaning program and a post-weaning program.

[0015] Further, according to the operating steps in step 2, the pre-weaning plan includes switching the weaning feed, pre-trial feeding at a fixed point and quantity for the first three days, entering the formal period from the fourth to the fifth day, adding feed twice a day at a fixed point, and allowing free access to food and water. The day before vaccination, more drinking water is added appropriately, and the pig house is sterilized and disinfected at the same time to maintain cleanliness, reduce the temperature difference and humidity changes between day and night, and gradually increase the frequency of daily personnel inspections.

[0016] Furthermore, according to the operating steps in step two, the post-weaning plan includes increasing the frequency of personnel inspections, and then conducting environmental adaptation for each group every day, sterilizing and disinfecting the pig houses simultaneously, maintaining cleanliness, reducing the temperature difference and humidity changes between day and night, and gradually increasing the frequency of daily personnel inspections.

[0017] Further, according to the operating steps in step three, the inoculation amounts of the classical swine fever vaccine and the original branch double vaccine are 1ds and 2ml.

[0018] Further, according to the operating steps in step three, the inoculation environment registration variables are set to light, air flow rate in the house, noise, temperature and humidity.

[0019] Further, according to the operating steps in step 4, the dosages of the pseudorabies vaccine and foot-and-mouth disease vaccine are 1 ds and 2 ml, respectively.

[0020] Furthermore, according to the operating steps in step 4, the inoculation environment is optimized and controlled, and the environmental registration variables on the day of inoculation are set as indicators for daily breeding.

[0021] Furthermore, according to the operating steps in step six, the sampling index is that the sampling ratio of each group reaches 5%.

[0022] The present invention provides an immunization method under non-swine fever conditions, which has the following beneficial effects: the immunization method under non-swine fever conditions, by grouping piglets and injecting multiple vaccines simultaneously, can reduce the generation of stress reactions, and at the same time, before vaccination, can further gradually adapt to avoid the generation of stress reactions during the vaccination process, and at the same time reduce excessive stress reactions caused by weaning, and in the subsequent observation process, can prevent vaccination, reduce the number of inadaptations caused by vaccinated piglets, and appropriately control the breeding environment to avoid infection and diseases caused by needle holes. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The present invention is a schematic flow chart of an immunization method under non-swine fever conditions. DETAILED DESCRIPTION

[0024] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments.

[0025] An immunization method under non-swine fever conditions comprises the following steps:

[0026] Step 1: First, group the one-day-old piglets into groups A, B, C and D.

[0027] Step 2: On the 35th day, 56th day and five days before the 90th day, switch the breeding plan to adapt to the stress response.

[0028] Step 3. On the 35th day, select Group A for vaccination with Classical Chinese Fever vaccine and the original branch two-shot vaccine, record the vaccination environment, observe the stress response of Group A, optimize and control the vaccination environment, and complete the vaccination of Groups B, C and D with Classical Chinese Fever vaccine and the original branch two-shot vaccine.

[0029] Step 4. On the 56th day, after Group A is eliminated, select one group at random, record the vaccination environment, vaccinate with pseudorabies and foot-and-mouth disease vaccines, observe the stress response, optimize and control the vaccination environment, and complete the vaccination of the remaining three groups with pseudorabies and foot-and-mouth disease vaccines.

[0030] Step 5. On the 90th day, after excluding Group A and the 56th day priority vaccination group, choose one of the remaining two groups and vaccinate them with pseudorabies and foot-and-mouth disease vaccines, observe the stress response, optimize and control the vaccination environment, and complete the vaccination of the remaining three groups with pseudorabies and foot-and-mouth disease vaccines.

[0031] Step 6. Ten days after each vaccination, random sampling will be conducted to test antibody concentration and determine the effectiveness of the vaccination.

[0032] Specifically, according to the operating procedures in step one, one-day-old piglets need to be sampled in advance to make sure they are not infected with the virus to ensure healthy breeding.

[0033] Specifically, according to the operating steps in step 2, the stress response adaptation breeding program is divided into a pre-weaning program and a post-weaning program.

[0034] Specifically, according to the operating procedures in step two, the pre-weaning plan includes switching weaning feed, pre-trial feeding at a fixed location and quantity for the first three days, entering the formal period from the fourth to the fifth day, adding feed twice a day at a fixed point, and allowing free access to food and water. The day before vaccination, more drinking water should be added appropriately, and the pig house should be sterilized and disinfected at the same time to maintain cleanliness, reduce the temperature difference and humidity changes between day and night, and gradually increase the frequency of daily personnel inspections.

[0035] Specifically, according to the operating procedures in step two, the post-weaning plan includes increasing the frequency of personnel inspections, and then conducting environmental adaptation for each group every day, sterilizing and disinfecting the pig houses at the same time to maintain cleanliness, reduce the temperature difference and humidity changes between day and night, and gradually increase the frequency of daily personnel inspections.

[0036] Specifically, according to the operating steps in step three, the inoculation amounts of classical swine fever vaccine and the original double vaccine are 1ds and 2ml.

[0037] Specifically, according to the operating steps in step three, the inoculation environment registration variables are set to light, air flow rate in the house, noise, temperature and humidity.

[0038] Specifically, according to the operating steps in step 4, the dosages of pseudorabies vaccine and foot-and-mouth disease vaccine are 1 ds and 2 ml respectively.

[0039] Specifically, according to the operating steps in step 4, the inoculation environment is optimized and controlled, and the environmental registration variables on the day of inoculation are set as indicators for daily breeding.

[0040] Specifically, according to the operating procedures in step six, the sampling inspection indicator is that the sampling inspection ratio of each group reaches 5%.

[0041] The purity detection method of the embodiment is tested and analyzed, and compared with the prior art, the following data are obtained:

[0042] Vaccination risks Degree of stress response Needle eye control Example Lower Lower better Prior art Higher generally generally

[0043] According to the data in the above table, it can be concluded that when the embodiment is implemented, through the immunization method under non-swine fever conditions of the present invention, the immunization method under non-swine fever conditions, by grouping piglets and injecting multiple vaccines simultaneously, can reduce the occurrence of stress response, and at the same time, before vaccination, further gradual adaptation can be achieved to avoid stress response during the vaccination process, while reducing excessive stress response caused by weaning. In the subsequent observation process, vaccination can be prevented, the number of inadaptations caused by vaccinated piglets can be reduced, and the breeding environment can be properly controlled to avoid infection and disease caused by needle holes.

[0044] The present invention provides an immunization method under non-swine fever conditions, comprising the following steps:

[0045] Step 1: First, group the one-day-old piglets into groups A, B, C and D. One-day-old piglets need to be sampled in advance to confirm that they are not infected with the virus to ensure the health of the piglets. Step 2: On the 35th, 56th and 90th days, switch the stress response adaptation breeding plan. The stress response adaptation breeding plan is divided into a pre-weaning plan and a post-weaning plan. The pre-weaning plan includes switching weaning feed, fixed-point quantitative pre-trial feeding for the first three days, and entering the formal period from the fourth to the fifth day. Feed is added at a fixed point twice a day, and the piglets are free to eat and drink water. One day before vaccination, more drinking water is added appropriately, and the pig house is killed at the same time. Sterilize, maintain cleanliness, reduce the temperature difference between day and night and humidity changes, and gradually increase the frequency of daily personnel inspections. The post-weaning plan includes increasing the frequency of personnel inspections, and then carrying out environmental adaptation for each group every day, sterilizing and disinfecting the pig house at the same time, maintaining cleanliness, reducing the temperature difference between day and night and humidity changes, and gradually increasing the frequency of daily personnel inspections. Late weaning is the traditional feeding method in the past, in which the time from the birth of piglets to the stop of breastfeeding is about 35-55 days. This method is basically no longer used. The so-called early weaning is the generally recognized method of weaning when the piglets weigh 4.When the sow weighs more than 5 kg, that is, 14-35 days, early weaning can shorten the sow's reproductive cycle and increase the number of litters. The appropriate age for early weaning of piglets can be determined according to production tasks and production levels. Generally, it is appropriate to wean introduced breeds at 28 days and local breeds at 35 days. This is the weaning time for piglets in most parts of my country. Step three, at 35 days, select Group A for vaccination with classical swine fever vaccine and original branch two-in-one vaccine, record the vaccination environment, observe the stress response of Group A, optimize and control the vaccination environment, complete the vaccination of Group B, C and D with classical swine fever vaccine and original branch two-in-one vaccine, the inoculation amount of classical swine fever vaccine and original branch two-in-one vaccine is 1ds and 2ml, and the registration variables of the vaccination environment are set to, light, air flow rate in the house, noise, temperature and humidity , Classical swine fever live vaccine is used to prevent classical swine fever. It can be used alone or in combination with anti-classic swine fever serum for emergency prevention in pig herds infected with classical swine fever. This vaccine is made by inoculating healthy rabbits or suckling rabbits with a rabbit-attenuated strain of classical swine fever virus. The spleen and lymph nodes of infected rabbits, or the muscles and solid organs of suckling rabbits, are harvested, ground into a homogenate, and then an appropriate stabilizer is added. The vaccine is made by freeze-vacuum drying. Step 4. At 56 days, after group A is discharged, one group is randomly selected, the vaccination environment is recorded, pseudorabies and foot-and-mouth disease vaccines are inoculated, the stress response is observed, the vaccination environment is optimized and controlled, and the vaccination of the remaining three groups of pseudorabies and foot-and-mouth disease vaccines is completed. The dosage of pseudorabies and foot-and-mouth disease vaccines is 1ds and 2ml respectively. The vaccination environment is optimized and controlled. The environmental registration variables on the day of vaccination are set It is an indicator for daily breeding. Piglets with pseudorabies have the highest mortality rate. Pregnant sows often miscarry after being infected with the disease. In addition, healthy adult boars are usually potential carriers. They can carry and spread the virus without showing symptoms or suddenly becoming unusable. Pigs (including domestic pigs and wild boars) are usually the reservoir hosts of this virus. Although the virus can also infect other species, the mortality rate is usually high. Pseudorabies has also been reported in other mammals including American black bears, Florida panthers, raccoons, coyotes, and white-tailed deer. In most cases, they are or are suspected to be related to contact with pigs or pig products. Outbreaks of the disease in domesticated fur animals (mink or fox) in Europe are related to products made from sick pigs. Some other species can be tested through testing. Test for infection, humans are not potential hosts, step five, at 90 days, exclude group A and the 56-day priority vaccination group, and choose one of the remaining two groups, vaccinate with pseudorabies and foot-and-mouth disease vaccine, observe stress response, optimize and control the vaccination environment, and complete the vaccination of the remaining three groups with pseudorabies and foot-and-mouth disease vaccine. Foot-and-mouth disease is an acute, febrile, highly contagious disease shared by major livestock such as pigs, cattle, and sheep and other domestic and wild even-toed ungulates. There are more than 70 susceptible animals. The clinical feature is the occurrence of vesicular rash on the oral mucosa, hoofs, and breast skin. The disease has many transmission routes and is fast. Step six, ten days after each vaccination, conduct random inspections, random inspections of antibody concentrations, and determine the vaccination effect. The inspection index is that the sampling ratio of each group reaches 5%. .

[0046] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An immunization method under non-swine fever conditions, It is characterized in that The following steps are involved: S1. First, one-day-old piglets were divided into groups A, B, C and D; S2, on the 35th, 56th and five days before the 90th day, switch breeding to adapt the breeding scheme to stress response; S3. On the 35th day, select group A to be vaccinated with classical swine fever vaccine and the original ramification double vaccine, record the inoculation environment, observe the stress response of group A, optimize and control the inoculation environment, and complete the inoculation of groups B, C and D with classical swine fever vaccine and the original ramification double vaccine; S4. On day 56, after excluding group A, randomly select one group as the priority vaccination group on day 56, record the vaccination environment, vaccinate with pseudorabies and foot-and-mouth disease vaccines, observe stress reactions, optimize and control the vaccination environment, and complete the vaccination of the remaining three groups with pseudorabies and foot-and-mouth disease vaccines; S5. At 90 days, exclude group A and the 56-day priority vaccination group, and choose one of the remaining two groups to be vaccinated with pseudorabies and foot-and-mouth disease vaccines, observe the stress response, optimize and control the vaccination environment, and complete the vaccination of the remaining three groups with pseudorabies and foot-and-mouth disease vaccines; S6. Ten days after each vaccination, random inspections will be conducted to check antibody concentrations and determine the effectiveness of the vaccination; The stress response adaptation breeding program is divided into a pre-weaning program and a post-weaning program; The pre-weaning program includes switching the weaning feed, pre-test feeding at a fixed location and quantity for the first three days, entering the formal period from the fourth to the fifth day, adding feed at a fixed location twice a day, free access to food and water, and adding more drinking water one day before vaccination. The pig house is sterilized and disinfected at the same time to maintain cleanliness, reduce the temperature difference between day and night and the change of humidity, and gradually increase the frequency of daily personnel inspections; The post-weaning plan includes increasing the frequency of personnel inspections, followed by environmental adaptation for each group every day, simultaneous sterilization and disinfection of the pig houses to maintain cleanliness, reducing the temperature difference and humidity changes between day and night, and gradually increasing the frequency of daily personnel inspections.

2. The immunization method under non-swine fever conditions according to claim 1, It is characterized in that The following steps are involved: According to the operating steps in S1, the one-day-old piglets need to be sampled in advance to make sure they are not infected with the virus and to ensure the health of the piglets.

3. The immunization method under non-swine fever conditions according to claim 1, It is characterized in that The following steps are involved: According to the operating steps in S3, the inoculation amounts of the classical swine fever vaccine and the original double vaccine are 1 ds and 2 ml respectively.

4. The immunization method under non-swine fever conditions according to claim 1, It is characterized in that The following steps are involved: According to the operation steps in S3, the registration variables for optimizing and controlling the inoculation environment are set to light, air flow rate in the house, noise, temperature and humidity.

5. The immunization method under non-swine fever conditions according to claim 1, It is characterized in that The following steps are involved: According to the operation steps in S4, the inoculation amounts of the pseudorabies vaccine and foot-and-mouth disease vaccine are 1 ds and 2 ml, respectively.

6. The immunization method under non-swine fever conditions according to claim 1, It is characterized in that The following steps are involved: According to the operation steps in S4, the inoculation environment is optimized and controlled, and the environmental registration variables on the day of inoculation are set as indicators for daily breeding.

7. The immunization method under non-swine fever conditions according to claim 1, It is characterized in that The following steps are involved: According to the operation steps in S6, the sampling ratio of each group is greater than 5%.

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