Breeding method of low birth weight Hu lambs
By using sodium butyrate feeding method in low-birth heavy lake lambs, the problems of slow growth and low immune function of low-birth heavy lake lambs were solved, and their growth performance and antioxidant ability were significantly improved.
Patent Information
- Application Number
- CN202510333876.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-20
- Publication Date
- 2025-06-20
AI Technical Summary
After birth, low-birth heavy lake lambs show slow growth, abnormal development, decreased disease resistance, high early incidence and mortality, which affects the efficiency of breeding production.
A breeding method is adopted. Lambs are fed breast milk in time after they are born, and they start feeding sodium butyrate at 5 days old. The feeding amount is adjusted according to their weight. The weight is measured every week. The sodium butyrate is fed for 30 days to 35 days old and weaned.
Through this method, the developmental delay of low-birth heavy lake lambs was relieved, the body's immune function and antioxidant ability were significantly improved, and the growth performance was also significantly improved.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of animal husbandry, and in particular relates to a breeding method for low-birth-weight Hu sheep lambs. Background Art
[0002] Low birth weight, also known as intrauterine growth retardation, refers to the general term for a series of growth disorders that occur after birth, resulting in the fetus and its organs of mammals being affected by various factors in the mother's uterus, causing the newborn's initial birth weight to be lower than normal. Studies have shown that low birth weight animals show growth retardation, developmental abnormalities, decreased disease resistance, high early morbidity and mortality, and can even affect the growth and health of animals in the long term through "fetal programming", reducing the efficiency of breeding production. Low birth weight, or intrauterine growth retardation, has a high incidence in human and animal production, accounting for about 5%-10% of pregnancies.
[0003] Huyang is an excellent local sheep breed with excellent traits such as early sexual maturity, high fertility, tender meat, less mutton smell, and adaptability to long-term confinement. In addition, Huyang is also a world-renowned multi-birth sheep breed, with more than 70% of adult ewes giving birth to twins, and more than 30% giving birth to three or four lambs. However, ewes pregnant with multiple lambs have a heavy burden on their bodies, and there are often individuals with low birth weight in the same litter. Through statistics on the birth weight of lambs born within seven or eight months in large-scale sheep farms (626 lambs), the number of low-birth-weight lambs, that is, lambs with intrauterine growth retardation, accounted for nearly 10%. Based on the principle of "giving birth to more and raising all", how to ensure the healthy growth of low-birth-weight lambs and improve their growth performance plays a vital role in improving the economic benefits of farms and increasing the economic income of farmers. Summary of the invention
[0004] The object of the present invention is to provide a breeding method for low-birth-weight Hu sheep lambs, which can achieve the healthy growth of low-birth-weight Hu sheep lambs, improve the immune function and antioxidant capacity of the animal body, and thus improve the production performance.
[0005] The technical solution adopted by the present invention to solve its technical problem is:
[0006] A breeding method for low-birth-weight Hu sheep lambs, the method specifically comprising:
[0007] Lambs should eat breast milk promptly after birth and live with the ewes;
[0008] Starter feed was added at 5 days of age and sodium butyrate was gavage-fed. The starter feed was provided to the lambs ad libitum. The gavage-fed method of sodium butyrate was as follows: 40 g of sodium butyrate was dissolved in 100 ml of normal saline to prepare a solution with a final concentration of 0.4 g / ml. Each lamb was gavage-fed with 0.8 ml / kg to 1 ml / kg of body weight of sodium butyrate.
[0009] Measure the body weight once a week and adjust the gavage amount of sodium butyrate according to the body weight change;
[0010] Stop gavage of sodium butyrate at 30 to 35 days of age and wean the lambs.
[0011] Preferably, low birth weight lambs refer to lambs with a birth weight lower than 1.5 standard deviations of the overall average body weight of the lambs.
[0012] Preferably, to ensure the effect of sodium butyrate and avoid dilution of sodium butyrate by breast milk, separate the lambs from the ewes for 1 hour before and after gavage of sodium butyrate.
[0013] Preferably, the starter feed is granular feed, and the nutritional components include crude protein ≥ 18% and crude fiber ≤ 15%. The starter feed is a conventional commercially available product.
[0014] The beneficial effects of the present invention are as follows: The present invention applies sodium butyrate to the growth process of low birth weight Hu sheep lambs, so that the growth retardation of low birth weight lambs is well alleviated, and the body immune function, antioxidant capacity and growth performance are all well improved. By adopting the breeding method of the present invention, the healthy growth of low birth weight Hu sheep lambs is realized, the immune function and antioxidant capacity of the animal body are improved, and thus the growth performance is improved. Specific Embodiments
[0015] The following are specific examples to further illustrate the technical solutions of the present invention. It should be understood that the implementation of the present invention is not limited to the following examples, and any form of modification and / or change made to the present invention will fall within the protection scope of the present invention.
[0016] In the present invention, unless otherwise specified, all parts and percentages are in weight units, and the equipment and raw materials used can be purchased from the market or are commonly used in the art. The methods in the following examples are all conventional methods in the art unless otherwise specified.
[0017] The reagents used in the following examples can be purchased from conventional biochemical reagent stores unless otherwise specified.
[0018] Sodium butyrate is uncoated sodium butyrate provided by Hangzhou Kangdequan Co., Ltd., and its physical property is white powder, which is easily soluble in water.
[0019] The starter feed is purchased from Cargill Feed (Hefei) Co., Ltd., and the nutritional components include crude protein ≥ 18% and crude fiber ≤ 15%.
[0020] Examples
[0021] Experimental method: Twenty-four neonatal Hu sheep lambs with a birth weight below 1.5 times the standard deviation of the overall average were selected and divided into two groups, a control group and a sodium butyrate group. The sodium butyrate group was orally administered 1 ml / kg body weight of sodium butyrate, and the control group was administered the same volume of normal saline. Starting from 5 days of age, it was administered once a day at 08:00. Before and after administration, the lambs were separated from the ewes for 1 hour, and freely suckled for the rest of the time. The administration volume was adjusted once a week according to the body weight measured. Sodium butyrate was dissolved in normal saline at a final concentration of 0.4 g / mL. The experimental period was 30 days. During the experiment, the lambs had free access to water and freely ate the starter feed. The administration of sodium butyrate was stopped at 35 days of age.
[0022] 1. Determination of growth performance
[0023] On the start and end days of the feeding experiment, the lambs were weighed on an empty stomach before morning feeding, and the average daily gain (ADG) of the lambs was calculated.
[0024] 2. Determination of slaughter performance
[0025] After the feeding experiment ended, 5 lambs closest to the average body weight within each group were selected for slaughter. Before slaughter, they were fasted and water-deprived and weighed, which was the live weight before slaughter. After slaughter, the heart, liver, spleen, lungs, and kidneys were separated and weighed. After the rumen, reticulum, omasum, and abomasum were separated, the contents were rinsed clean with normal saline and weighed, and the organ index was calculated (organ index = organ weight / live weight before slaughter). The lambs were decapitated, skinned, hoofed, tailed, and eviscerated, and the carcass weight (including the kidneys) was weighed after standing for half an hour, and the slaughter rate was calculated.
[0026] 3. Determination of plasma biochemical, immune, and antioxidant indexes
[0027] On the end day of the experiment, 2 hours after the lambs were administered sodium butyrate, blood samples were collected from the jugular vein using heparin sodium blood collection tubes. The collected blood was centrifuged at 3000 rpm for 15 minutes at 4°C to separate the plasma, which was stored at -20°C.
[0028] The biochemical indexes in the plasma included total cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein, glucose, and urea nitrogen. The antioxidant indexes included total antioxidant capacity, catalase, glutathione peroxidase, superoxide dismutase, and malondialdehyde assay kits, all of which were purchased from Beijing Huaying Biological Research Institute; the immune indexes included growth hormone, immunoglobulin A, immunoglobulin G, immunoglobulin M, interleukin-6, interleukin-10, tumor necrosis factor α, and D-lactic acid assay kits, all of which were purchased from Hangzhou Jinhengnuo Biotechnology Co., Ltd. The specific experimental procedures were strictly in accordance with the kit instructions.
[0029] 4. Determination of liver lipid metabolism and antioxidant indexes
[0030] After the lambs were slaughtered, the liver was separated and stored in a cryotube at -80°C.
[0031] The assay kits for triglyceride, hepatic lipase, lipoprotein lipase, total lipase, total cholesterol and total bile acid, which are enzymes related to lipid metabolism in the liver, were all purchased from Beijing Huaying Biological Research Institute. The specific experimental procedures were strictly in accordance with the kit instructions. The determination parameters and methods for antioxidant indexes were the same as those for plasma.
[0032] 5. Determination of antioxidant indexes in jejunal epithelium
[0033] After the lambs were slaughtered, the jejunal segments were rinsed with normal saline, then dissected with scissors, and the jejunal epithelium was scraped onto a glass slide and stored in a cryotube at -80 °C.
[0034] The determination parameters and methods for antioxidant indexes in jejunal epithelium were the same as those for plasma.
[0035] A. Effects of adding sodium butyrate on the growth performance of low-birth-weight Hu sheep lambs
[0036] The results of the birth weight (kg), initial weight (kg) and final weight (kg) of Hu sheep lambs are shown in Table 1. The birth weights of lambs in the control group and the sodium butyrate group were significantly lower than those of normally growing lambs (2.03 kg vs 2.96 kg), and there was no significant difference in the initial weights of the two groups of lambs (P>0.05). The final weight of low-birth-weight lambs increased significantly after adding sodium butyrate (P<0.05); the average daily gain of the sodium butyrate group was significantly higher than that of the control group (P<0.05). Thus, it can be seen that gavage with sodium butyrate can significantly improve the growth performance of low-birth-weight lambs.
[0037] Table 1. Effects of adding sodium butyrate on the growth performance of low-birth-weight Hu sheep lambs
[0038]
[0039] Note: Data in the same row without letter superscripts indicate no significant difference (P>0.05), and different lowercase letters indicate significant difference (P<0.05). The same applies to the following tables.
[0040] The slaughter performance data of Hu sheep lambs after the end of the experimental period are shown in Table 2. Compared with the control group, the small intestine index of lambs in the sodium butyrate group increased significantly (Table 2, P<0.05), and there was a tendency for the rumen index (P = 0.057) and reticulum index (P = 0.087) to increase. Adding sodium butyrate could significantly increase the carcass weight (P<0.05) and dressing percentage (P<0.05) of lambs.
[0041] Table 2. Effects of adding sodium butyrate on the slaughter performance of low-birth-weight Hu sheep lambs
[0042]
[0043]
[0044] B. Effects of Adding Sodium Butyrate on Blood Indexes of Low Birth Weight Hu Sheep Lambs
[0045] On the day when the experiment ended, blood was collected from the jugular vein 2 h after intragastric administration of sodium butyrate, and the biochemical indexes, hormones and antioxidant indexes in plasma were measured as shown in Table 3, Table 4 and Table 5 respectively.
[0046] Table 3. Effects of Adding Sodium Butyrate on Plasma Biochemical Indexes of Low Birth Weight Hu Sheep Lambs
[0047]
[0048] Table 4. Effects of Adding Sodium Butyrate on Plasma Hormone Levels of Low Birth Weight Hu Sheep Lambs
[0049]
[0050] Table 5. Effects of Adding Sodium Butyrate on Plasma Antioxidant Indexes of Low Birth Weight Hu Sheep Lambs
[0051]
[0052] The results showed that adding sodium butyrate could increase the content of glucose in the plasma of low birth weight lambs (Table 3, P<0.05), indicating that sodium butyrate was beneficial to repair the energy metabolism of low birth weight lambs.
[0053] Adding sodium butyrate could significantly increase the level of growth hormone in the plasma of low birth weight lambs (Table 4, P<0.05), indicating that sodium butyrate could promote the growth of low birth weight lambs, which was also a possible reason for the improvement of growth performance in low birth weight lambs after intragastric administration of sodium butyrate. Adding sodium butyrate could reduce the levels of pro-inflammatory factors interleukin-1β and interleukin-6 in the plasma of lambs (P<0.05) and increase the contents of immunoglobulins A, G, and M (P<0.05), thus enhancing the immune function of low birth weight lambs. Compared with the control group, the content of D-lactic acid in the plasma of lambs in the sodium butyrate group showed a downward trend (P = 0.062). D-lactic acid is the metabolic end product of indigenous bacteria in the gastrointestinal tract, and mammals do not have an enzyme system that can quickly decompose it. When the intestinal mucosal biological barrier is damaged, a large amount of D-lactic acid produced by bacteria in the intestine penetrates through the damaged intestinal mucosa into the blood circulation. Therefore, the plasma D-lactic acid level can timely reflect the degree of intestinal mucosal injury and permeability changes, which is a prominent manifestation of intestinal permeability change and intestinal mucosal dysfunction. Therefore, it can be shown that adding sodium butyrate can relieve the intestinal injury of low birth weight lambs. During the experiment, the diarrhea rate of lambs in the sodium butyrate group was significantly lower than that in the control group.
[0054] Adding sodium butyrate can improve the plasma antioxidant indexes of low birth weight lambs, increase the total antioxidant capacity (Table 5, P<0.05), improve the peroxidase activity (P<0.05), and there is a tendency for the activities of glutathione peroxidase (P = 0.06) and superoxide dismutase (P = 0.08) to increase.
[0055] C. Effects of Adding Sodium Butyrate on Lipid Metabolism and Antioxidation in the Liver of Low Birth Weight Hu Sheep Lambs
[0056] Compared with the control group, there is a tendency for the concentration of triglycerides in the liver of lambs in the sodium butyrate group to increase (Table 6, P = 0.063). As the central regulator of lipid homeostasis, the liver is responsible for de novo synthesis, oxidation, and output of fatty acids. Adding sodium butyrate can increase the activities of lipoprotein lipase and total lipase in the liver (P<0.05). Thus, it can be seen that sodium butyrate can alleviate the disorder of lipid metabolism in the liver of low birth weight lambs.
[0057] Table 6. Effects of Adding Sodium Butyrate on Plasma Lipid Metabolism in the Liver of Low Birth Weight Hu Sheep Lambs
[0058]
[0059] Adding sodium butyrate can improve the antioxidant indexes in the liver of low birth weight lambs (Table 7), increase the total antioxidant capacity (P<0.05), improve the activities of catalase (P<0.05) and superoxide dismutase (P<0.05), and there is a tendency for the activity of glutathione peroxidase (P = 0.06) to increase. It shows that adding sodium butyrate has a significant effect on improving the antioxidant capacity of the liver of low birth weight lambs.
[0060] Table 7. Effects of Adding Sodium Butyrate on Antioxidant Indexes in the Liver of Low Birth Weight Hu Sheep Lambs
[0061]
[0062] D. Effects of Adding Sodium Butyrate on Antioxidation in the Jejunum of Low Birth Weight Hu Sheep Lambs
[0063] Adding sodium butyrate can improve the antioxidant indexes in the jejunum of low birth weight lambs (Table 8), increase the activities of catalase, glutathione peroxidase and superoxide dismutase (P<0.05), and there is a tendency for the concentration of malondialdehyde (P = 0.063) to decrease.
[0064] Table 8. Effects of Adding Sodium Butyrate on Antioxidant Indexes in the Jejunum of Low Birth Weight Hu Sheep Lambs
[0065]
[0066]
[0067] Conclusion: The method of the present invention applies sodium butyrate to the growth and development of low-birth-weight Hu sheep lambs. Through systematic experimental research, it is confirmed that this method can effectively alleviate the growth retardation problem of low-birth-weight lambs, specifically manifested as: significantly improving the disordered state of liver lipid metabolism, enhancing the body's immune function, and at the same time enhancing the antioxidant capacity of tissues such as blood, liver, and intestine. Finally, the growth performance and slaughter performance of the lambs in the experimental group were significantly improved. The breeding method of the present invention has successfully achieved the healthy growth of low-birth-weight Hu sheep lambs, and by enhancing the immune function and antioxidant capacity of the animal body, the purpose of improving the growth performance is achieved.
[0068] In this specification, each embodiment is described in a progressive manner. The key point of each embodiment is to illustrate the differences from other embodiments. For the same or similar parts among the embodiments, reference can be made to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the description of the method part.
[0069] The above has introduced in detail a breeding method for low-birth-weight Hu sheep lambs provided by the present invention. Specific examples are used in this article to elaborate on the principle and implementation manner of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be noted that for those of ordinary skill in the art in this technical field, without departing from the principle of the present invention, several improvements and modifications can still be made to the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention.
Claims
1. A method for breeding low-birth-weight Hu sheep lambs, characterized in that The method is specifically: Lambs should eat breast milk promptly after birth and live with the ewes; Starter feed was added at 5 days of age and sodium butyrate was gavage-fed. The starter feed was provided to the lambs ad libitum. The gavage-fed method of sodium butyrate was as follows: 40 g of sodium butyrate was dissolved in 100 ml of normal saline to prepare a solution with a final concentration of 0.4 g / ml. Each lamb was gavage-fed with 0.8 ml / kg to 1 ml / kg of body weight of sodium butyrate. Body weight was measured once a week, and the amount of sodium butyrate administered was adjusted according to the changes in body weight; Sodium butyrate gavage should be stopped at 30 to 35 days of age, and the pigs should be weaned.
2. The breeding method of low birth weight Hu sheep lambs according to claim 1, characterized in that: Low birth weight lambs refer to lambs whose birth weight is less than 1.5 standard deviations of the overall average weight of lambs.
3. The breeding method of low birth weight Hu sheep lambs according to claim 1, characterized in that: To ensure that sodium butyrate is effective and avoid dilution of sodium butyrate by breast milk, the lambs were separated from the ewes for 1 hour before and after sodium butyrate was gavage.
4. The breeding method of low birth weight Hu sheep lambs according to claim 1, characterized in that: The starter feed is a granular feed, comprising crude protein ≥ 18% and crude fiber ≤ 15%.