A preparation device for sophora spina flavone nanomicelles

Abstract

The present application discloses a preparation device for sophora spinosa flavone nanomicelles, which relates to the technical field of nanomicelle preparation. It includes a main stirring device, and the main stirring device includes a stirring ring assembly; it also includes an auxiliary stirring device; the auxiliary stirring device can be fixed on the stirring ring assembly by means of threaded connection; the auxiliary stirring device includes a connecting ring, a water-permeable ring and a bottom shell; the connecting ring is annular, and the outer diameter of the connecting ring is smaller than the inner diameter of the container; the upper end of the water-permeable ring is fixedly connected to the lower end of the connecting ring, and the shape of the water-permeable ring is annular; the bottom shell is a hollow cylinder with an opening at the upper part as a whole, and the bottom surface bulges downward into an arch shape, and the upper end opening of the bottom shell is fixedly connected to the lower end of the water-permeable ring; a plurality of stoppers are uniformly arranged on the inner wall of the bottom shell, and the bottom shell is provided with a central hole; it can achieve the technical effect that when the depth of the solution in the container is relatively deep, the air pump blows air towards the liquid surface in the container to drive the solution to rotate, the rotation effect of the solution is better, and the stirring and mixing efficiency of the solution is relatively high.

Description

Technical Field

[0001] The present invention relates to the technical field of the preparation of nano micelles, and particularly to a preparation device for sophora flavone nano micelles. Background Art

[0002] As a member of the carrier drug delivery system, polymer micelles developed from amphiphilic block copolymers have been deeply favored in the past decade. When put into an aqueous medium, driven by the reduction of the free energy of the system, the hydrophobic segments of the micelles spontaneously aggregate together to form the core of the microparticles. It can not only serve as a microreservoir for many poorly soluble drugs, but also avoid the inactivation of drugs in the biological internal environment.

[0003] When preparing drug-loaded nano micelles in small batches, the block copolymer and the drug are dissolved in an organic solvent, and the mixed solution needs to be stirred and dissolved and then the organic solvent is removed. For example, the Chinese invention patent with the authorization announcement number CN220732277B discloses a multifunctional solution stirring device, which is provided with a telescopic rod, an air pump and a controller on a bearing platform; the telescopic rod supports a stirring ring through a crank, and an annular cavity and an inclined hole are opened inside the stirring ring. When the air pump works, the inclined hole can blow air towards the liquid surface in the container to drive the solution to rotate.

[0004] Although the above device can blow the liquid surface in the container to drive the solution to rotate for mixing, when the depth of the solution in the container is relatively deep, the rotation effect of the solution will be unsatisfactory, and the rotation effect of the solution when the air pump blows air towards the liquid surface in the container to drive the solution to rotate is unsatisfactory, and the stirring and mixing efficiency of the solution is relatively low. Summary of the Invention

[0005] By providing a preparation device for sophora flavone nano micelles in the embodiments of the present application, the technical problem in the prior art that when the depth of the solution in the container is relatively deep, the rotation effect of the solution when the air pump blows air towards the liquid surface in the container to drive the solution to rotate is unsatisfactory, and the stirring and mixing efficiency of the solution is relatively low is solved, and the technical effect that when the depth of the solution in the container is relatively deep, the rotation effect of the solution when the air pump blows air towards the liquid surface in the container to drive the solution to rotate is better, and the stirring and mixing efficiency of the solution is relatively high is achieved.

[0006] The embodiments of the present application provide a preparation device for sophora flavone nano micelles, including a main stirring device, and the main stirring device includes a stirring ring assembly; a sub-stirring device is also included;

[0007] The sub-stirring device can be fixed on the stirring ring assembly by means of threaded connection;

[0008] The sub-stirring device includes a connecting ring, a water seepage ring and a bottom shell;

[0009] The connecting ring is annular, located at the upper end of the sub-stirring device, and the outer diameter of the connecting ring is smaller than the inner diameter of the container;

[0010] The shape of the water seepage ring is annular, and the inner diameter and outer diameter of the water seepage ring are the same as those of the connection ring;

[0011] A number of through holes are evenly distributed on the water seepage ring;

[0012] The bottom shell is an overall hollow cylinder with an open upper end, and the bottom surface bulges downward into an arch shape. The upper end opening of the bottom shell is fixedly connected to the lower end of the water seepage ring, and the inner diameter and outer diameter of the upper end opening of the bottom shell are the same as those of the water seepage ring;

[0013] A central hole is opened at the bottom of the bottom shell for enabling the exchange and circulation of the solution inside and outside the bottom shell.

[0014] External threads are provided on the outer surface of the lower end of the stirring ring housing of the stirring ring assembly;

[0015] The spiral direction of the external threads on the outer surface of the stirring ring housing from top to bottom is opposite to the direction in which the stirring ring assembly blows air towards the liquid surface;

[0016] Internal threads meshing with the external threads on the stirring ring housing are provided on the connection ring, so that the secondary stirring device can be fixedly connected to the stirring ring assembly.

[0017] A number of stoppers are evenly arranged on the inner wall of the bottom shell;

[0018] The stopper is a cuboid, one end of the stopper is fixed on the inner wall of the bottom shell, and the other end extends towards the center of the bottom shell for making the dissolution and mixing of the solution inside the bottom shell more sufficient.

[0019] The secondary stirring device further includes a sliding assembly, an annular cavity and a communicating pipe;

[0020] The sliding assembly includes a spiral slide rail, a stop block, a first slider and a second slider;

[0021] The spiral slide rail is sleeved outside the secondary stirring device, the top end of the spiral slide rail is fixed on the connection ring, and extends vertically downward in a spiral shape;

[0022] The connection mode between the connection ring and the water seepage ring is a movable connection, and the water seepage ring can move away from and approach the connection ring along the spiral slide rail.

[0023] One end of the first slider close to the water seepage ring is fixed on the water seepage ring, and the other end away from the water seepage ring is placed on the spiral slide rail. The height of the first slider is two-thirds of the axial distance between the center lines of adjacent two turns of cross-sections on the spiral slide rail;

[0024] The size of the second slider is the same as that of the first slider, and the second slider is fixed on the other side of the water seepage ring relative to the position of the first slider;

[0025] The position of the first slider is higher than that of the second slider, and the height difference is half of the axial distance between the center lines of adjacent turns of the spiral slide rail in cross section, so that the first slider and the second slider can just slide up and down along the spiral slide rail;

[0026] The bottom end of the stop block is fixedly connected to the bottom end of the spiral slide rail, and the top end of the stop block protrudes upward, so that the second slider can stop when it slides to the bottom end of the spiral slide rail;

[0027] The spiral direction of the spiral slide rail from top to bottom is opposite to the direction in which the stirring ring assembly blows air to the liquid surface.

[0028] The lowest point at the bottom end of the spiral slide rail is on the same horizontal plane as the central hole. The inner diameter of the spiral slide rail is larger than the outer diameter of the connecting ring, and the outer diameter of the spiral slide rail is smaller than the inner diameter of the container.

[0029] The annular cavity is integrally circular and is fixed on the inner wall of the bottom of the bottom shell. The outer diameter of the annular cavity is smaller than the outer diameter of the bottom shell, the inner diameter of the annular cavity is larger than the radius of the central hole, the centers of the annular cavity and the central hole are on the same vertical line, the middle part of the annular cavity protrudes towards the center of the bottom shell, and the inside of the annular cavity is hollow;

[0030] The bottom shell further includes a rubber ring;

[0031] The rubber ring is integrally circular. The rubber ring is located on the bottom shell wrapped by the annular cavity. The inner ring of the rubber ring is placed on the bottom shell, and the outer ring is fixedly connected to the bottom shell, dividing the bottom shell into two parts, and these two parts are fixed by the annular cavity;

[0032] One side of the inner ring of the rubber ring close to the stop block protrudes towards the center of the circle, and the thickness of the rubber ring is greater than the thickness of the bottom shell.

[0033] The main body of the connecting pipe is rod-shaped, the bottom end is fixed on the outer ring of the annular cavity, and the other end extends vertically upward along the inner wall of the bottom shell to the junction of the water seepage ring and the bottom shell;

[0034] The inside of the connecting pipe is through and the inside of the connecting pipe is communicated with the inside of the annular cavity. The side of the connecting pipe close to the inner wall of the bottom shell is fixedly connected to the bottom shell together;

[0035] A communication port is opened at the top end of the connecting pipe, and the communication port is circular, so that gas or liquid can enter the connecting pipe through the communication port.

[0036] The container is provided with a raised block and an air intake assembly;

[0037] The raised block is rectangular in shape and is the part that protrudes from the bottom surface of the container towards the inside of the container;

[0038] The air intake assembly includes an intake pipe and a piercing needle;

[0039] The penetration needle vertically penetrates through the raised block and extends into the container. The penetration needle is located directly below the rubber ring, and the length of the penetration needle extending out of the raised block is greater than the maximum vertical distance between the rubber ring and the central hole.

[0040] The penetration needle is a through cylinder. The diameter of the cross-sectional circle of the penetration needle is 1 cm, and the top end of the penetration needle is needle-shaped, so that the penetration needle can better penetrate through the rubber ring.

[0041] The inside of the air inlet pipe is connected to the inside of the penetration needle. The air inlet pipe is embedded in the raised block, and the end far from the penetration needle is connected to the air pump assembly.

[0042] In the air pump assembly, in addition to the air pump that supplies air to the stirring ring assembly, there is also an air pump that supplies air to the air inlet pipe, and the two air pumps do not interfere with each other.

[0043] The number of the connecting pipes is two, and the two connecting pipes are symmetrically arranged.

[0044] The radius of the connecting port is half of the diameter of the penetration needle.

[0045] The width of the rubber ring is twice the diameter of the penetration needle.

[0046] One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:

[0047] By providing a secondary stirring device on the main stirring device, the secondary stirring device can be threadedly connected to the stirring ring assembly through a connecting ring. The secondary stirring device includes a water seepage ring and a bottom shell. The bottom shell separates a part of the solution to be stirred in the container, so that the stirring ring assembly can more easily blow the solution in the bottom shell. Through the water seepage ring and the central hole at the center of the bottom of the bottom shell, the solution inside and outside the bottom shell is exchanged; effectively solving the technical problem in the prior art that when the depth of the solution in the container is relatively deep, when the air pump blows air towards the liquid surface in the container to drive the solution to rotate, the rotation effect of the solution is not ideal and the stirring and mixing efficiency of the solution is relatively low. Furthermore, when the depth of the solution in the container is relatively deep, when the air pump blows air towards the liquid surface in the container to drive the solution to rotate, the rotation effect of the solution is better and the stirring and mixing efficiency of the solution is relatively high is achieved. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 It is a structural diagram of the preparation device of the sophora spina flavone nano micelle preparation device of the present invention;

[0049] Figure 2 It is a structural diagram of the main stirring device of the sophora spina flavone nano micelle preparation device of the present invention;

[0050] Figure 3 It is a structural diagram of the secondary stirring device of the sophora spina flavone nano micelle preparation device of the present invention;

[0051] Figure 4 Schematic diagram of the auxiliary stirring device of the preparation device for sophora spine flavone nanomicelles of the present invention;

[0052] Figure 5 Top view of the bottom shell of the preparation device for sophora spine flavone nanomicelles of the present invention;

[0053] Figure 6 Structural diagram of the sliding component of the preparation device for sophora spine flavone nanomicelles of the present invention;

[0054] Figure 7 Structural diagram of the annular cavity of the preparation device for sophora spine flavone nanomicelles of the present invention;

[0055] Figure 8 Structural diagram of the rubber ring of the preparation device for sophora spine flavone nanomicelles of the present invention;

[0056] Figure 9 Top view of the annular cavity of the preparation device for sophora spine flavone nanomicelles of the present invention;

[0057] Figure 10 Schematic diagram of the communication port of the preparation device for sophora spine flavone nanomicelles of the present invention;

[0058] Figure 11 Structural diagram of the air inlet component of the preparation device for sophora spine flavone nanomicelles of the present invention;

[0059] Figure 12 Top view of the container of the preparation device for sophora spine flavone nanomicelles of the present invention.

[0060] In the figure:

[0061] Shell 100, mixing chamber 110, vacuum drying chamber 120, dialysis chamber 130, wheel 140;

[0062] Main stirring device 200, base 210, weighing component 220, electric heating component 230, air pump component 240, telescopic component 250, crank arm 251, stirring ring component 260, stirring ring housing 261;

[0063] Auxiliary stirring device 300, connecting ring 310, water seepage ring 320, bottom shell 330, stop block 331, central hole 332, rubber ring 333, sliding component 340, spiral slide rail 341, stop block 342, first slider 343, second slider 344, annular cavity 350, connecting pipe 360, communication port 361;

[0064] Container 400, raised block 410, air inlet component 420, air inlet pipe 421, piercing needle 422. Detailed implementation manners

[0065] For the convenience of understanding the present invention, the present application will be described more comprehensively with reference to the relevant drawings; the preferred embodiments of the present invention are shown in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein; on the contrary, these embodiments are provided to make the disclosure of the present invention more thorough and comprehensive.

[0066] It should be noted that the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only embodiments.

[0067] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present invention belongs; the terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention; the term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0068] Please refer to Figure 2 , which is the structural diagram of the main stirring device of the preparation device for sophora spina flavone nanomicelles of the present invention. The preparation device for sophora spina flavone nanomicelles of the present application includes a stirring ring assembly 260, a secondary stirring device 300 and a container 400; the secondary stirring device 300 can be threadedly connected to the stirring ring assembly 260 through a connecting ring 310. The secondary stirring device 300 includes a water permeable ring 320 and a bottom shell 330. The bottom shell 330 separates a part of the solution to be stirred in the container 400, so that the stirring ring assembly 260 can more easily blow the solution in the bottom shell 330, and through the water permeable ring 320 and the central hole 332 at the center of the bottom of the bottom shell 330, the solution inside and outside the bottom shell 330 is exchanged; it solves the technical problem that when the depth of the solution in the container 400 is relatively deep, when the air pump blows air towards the liquid surface in the container 400 to drive the solution to rotate, the rotation effect of the solution is not ideal and the stirring and mixing efficiency of the solution is relatively low, and realizes the technical effect that when the depth of the solution in the container 400 is relatively deep, when the air pump blows air towards the liquid surface in the container 400 to drive the solution to rotate, the rotation effect of the solution is better and the stirring and mixing efficiency of the solution is relatively high.

[0069] Example 1

[0070] As Figure 1As shown in the figure, the preparation device of sophora spina flavone nanomicelles of the present application includes a housing 100, a main stirring device 200, a sub-stirring device 300 and a container 400; the housing 100 includes a mixing chamber 110, a vacuum drying chamber 120, a dialysis chamber 130 and wheels 140; the housing 100 divides the main body of the device into three areas, the middle area is the mixing chamber 110, and the vacuum drying chamber 120 and the dialysis chamber 130 are respectively on both sides of the mixing chamber 110; the main stirring device 200 and the sub-stirring device 300 are fixed inside the mixing chamber 110; a vacuum drying device is stored inside the vacuum drying chamber 120; a plurality of wheels 140 are installed below the housing 100 to facilitate the movement of the device.

[0071] As Figure 2 shown, the main stirring device 200 includes a base 210; a weighing component 220 is fixed on the base 210, which is used to support the electric heating component 230 and measure the weight change; the electric heating component 230 is fixed on the weighing component 220, the electric heating component 230 is circular in shape, and the container 400 for containing the mixed solution is placed above; the container 400 is cylindrical in shape; an air pump component 240 and a telescopic component 250 are fixed on the base 210; the air pump component 240 is located at the edge position of the base 210, and the upper part of the air pump component 240 is fixedly connected to the telescopic component 250, the telescopic component 250 extends vertically and its length can be adjusted manually, and a fixing bolt is provided on the telescopic component 250 to control the extension length of the telescopic component 250; the upper end of the telescopic component 250 is fixedly connected to the upper end of the crank arm 251; the crank arm 251 is L-shaped, and the lower end is fixedly connected with a stirring ring component 260; the stirring ring component 260 is annular in shape, the stirring ring component 260 is located directly above the electric heating component 230 and its outer diameter is smaller than the inner diameter of the container 400; by adjusting the length of the telescopic component 250, the height of the stirring ring component 260 can be adjusted, and the stirring ring component 260 can be adjusted into the container 400, and the stirring ring component 260 always remains horizontal; an annular cavity and a number of equally spaced inclined holes are provided inside the stirring ring component 260, the upper end of the inclined hole communicates with the annular cavity and the lower port is located on the lower end surface of the stirring ring component 260; both the telescopic component 250 and the crank arm 251 are hollow structures, and one end of the hollow cavity communicates with the annular cavity inside the stirring ring component 260, and the other end communicates with the air pump component 240, so that the air pump component 240 can convey gas into the stirring ring component 260 through the telescopic component 250 and the crank arm 251; the stirring ring component 260 includes a stirring ring housing 261; an external thread is provided on the lower outer surface of the stirring ring housing 261.

[0072] As Figure 3 、 Figure 4 and Figure 5As shown, the secondary stirring device 300 includes a connecting ring 310, a water seepage ring 320, and a bottom shell 330; the connecting ring 310 is annular, located at the upper end of the secondary stirring device 300, the outer diameter of the connecting ring 310 is smaller than the inner diameter of the container 400, and the inner diameter is equal to the outer diameter of the stirring ring assembly 260; the connecting ring 310 is provided with internal threads that mesh with the external threads on the outer surface of the stirring ring housing 261, so that the secondary stirring device 300 can be fixedly connected to the stirring ring assembly 260; the upper end of the water seepage ring 320 is fixedly connected to the lower end of the connecting ring 310, the water seepage ring 320 is annular in shape, and the inner diameter and outer diameter of the water seepage ring 320 are the same as the inner diameter and outer diameter of the connecting ring 310; a number of through holes are evenly distributed on the water seepage ring 320; the bottom shell 330 is an overall hollow cylinder with an open top, and the bottom surface bulges downward into an arch shape. The upper end opening of the bottom shell 330 is fixedly connected to the lower end of the water seepage ring 320, and the inner diameter and outer diameter of the upper end opening of the bottom shell 330 are the same as the inner diameter and outer diameter of the water seepage ring 320; a number of stoppers 331 are evenly arranged on the inner wall of the bottom shell 330; the stopper 331 is a cuboid, one end of the stopper 331 is fixed on the inner wall of the bottom shell 330, and the other end extends towards the center of the bottom shell 330, which is used to make the dissolution and mixing of the solution inside the bottom shell 330 more sufficient; a central hole 332 is opened at the bottom of the bottom shell 330.

[0073] Further, the spiral direction of the external threads on the outer surface of the stirring ring housing 261 from top to bottom is opposite to the direction in which the stirring ring assembly 260 blows air towards the liquid surface. That is, if the stirring ring assembly 260 blows air towards the liquid surface in a clockwise direction, the spiral direction of the external threads from top to bottom is counterclockwise; if the stirring ring assembly 260 blows air towards the liquid surface in a counterclockwise direction, the spiral direction of the external threads from top to bottom is clockwise; so that when the stirring ring assembly 260 blows air, it will not cause the secondary stirring device 300 to fall off the stirring ring housing 261.

[0074] Further, when installing the secondary stirring device 300, thread the secondary stirring device 300 onto the stirring ring assembly 260, and adjust the telescopic assembly 250 so that the bottom shell 330 is immersed in the internal solution of the container 400 and the water seepage ring 320 is located above the liquid surface of the solution.

[0075] Further, when the auxiliary stirring device 300 is installed on the stirring ring assembly 260 to stir the solution, the stirring ring assembly 260 blows air obliquely towards the liquid surface. At this time, only a part of the solution in the container 400 is placed in the auxiliary stirring device 300, and the depth of this part of the solution is the same as that of the bottom shell 330, so that the stirring ring assembly 260 can more effectively stir the solution when blowing towards the solution. When the solution is stirred, the height around is higher than the height in the center, and a part of the solution will seep out from the through holes on the water seepage ring 320. At this time, since a central hole 332 is opened in the middle of the bottom of the bottom shell 330 and a part of the solution on the bottom shell 330 seeps out, the solution below the bottom shell 330 will enter the bottom shell 330 through the central hole 332 for stirring and mixing.

[0076] When the sophora spine flavone nano-micelle preparation device of the embodiment of the present application is actually running, the steps are as follows:

[0077] S1: The operator first adds the absolute ethanol solution into the container 400, and then places the container 400 on the electric heating component 230;

[0078] S2: Then threadedly connect the auxiliary stirring device 300 to the stirring ring assembly 260, and adjust the telescopic component 250 so that the bottom shell 330 is immersed in the absolute ethanol and the water seepage ring 320 is located above the absolute ethanol liquid surface;

[0079] S3: Turn on the air pump in the air pump component 240 that communicates with the stirring ring assembly 260, so that the stirring ring assembly 260 blows air obliquely towards the liquid surface;

[0080] S4: Put the sophora spine flavone medicine and the block copolymer into the container 400 through the middle position of the stirring ring assembly 260 for stirring and mixing;

[0081] S5: After the mixing is completed, turn off the air pump component 240, disassemble the auxiliary stirring device 300 for cleaning, and at the same time turn on the electric heating component 230, set the temperature to 40 °C to heat the mixed liquid in the container 400 to evaporate into a film, and the excess absolute ethanol solution will volatilize;

[0082] S6: Add deionized water into the container 400, install the auxiliary stirring device 300 on the stirring ring assembly 260 to stir the solution in the container 400, and at the same time set the heating temperature of the electric heating component 230 to 60 °C for heating to form a micelle solution;

[0083] S7: Take out the micelle solution from the container 400 and put it into the dialysis chamber 130 for dialysis;

[0084] S8: Put the dialyzed micelles into the vacuum drying chamber 120 for vacuum freeze-drying to make freeze-dried micelles.

[0085] The technical solutions in the embodiments of the present application at least have the following technical effects or advantages:

[0086] It solves the technical problem in the prior art that when the depth of the solution in the container 400 is relatively deep, when the air pump blows air towards the liquid surface in the container 400 to drive the solution to rotate, the rotation effect of the solution is not ideal and the stirring and mixing efficiency of the solution is relatively low. It realizes the technical effect that when the depth of the solution in the container 400 is relatively deep, when the air pump blows air towards the liquid surface in the container 400 to drive the solution to rotate, the rotation effect of the solution is better and the stirring and mixing efficiency of the solution is relatively high; the auxiliary stirring device 300 can be easily detached from the main stirring device 200 for cleaning.

[0087] Embodiment 2

[0088] During the actual operation of the tumor diagnosis kit in the above embodiment, it is found that although the rotation effect of the solution is better when the air pump blows air towards the liquid surface in the container 400 through the auxiliary stirring device 300, due to the separation of the auxiliary stirring device 300, the dissolution and mixing effect between the upper layer solution and the lower layer solution in the container 400 is not ideal, and there is no effective circulation and exchange between the upper layer solution and the lower layer solution. The embodiments of the present application optimize the structures of the auxiliary stirring device 300 and the container 400 on the basis of the above embodiments.

[0089] Such as Figure 6As shown, the auxiliary stirring device 300 further includes a sliding assembly 340, an annular cavity 350, and a connecting pipe 360; the sliding assembly 340 includes a spiral slide rail 341, a stop block 342, a first slider 343, and a second slider 344; the spiral slide rail 341 is sleeved outside the auxiliary stirring device 300, and the top end of the spiral slide rail 341 is fixed on the connecting ring 310 and extends vertically downward in a spiral shape; the connection between the connecting ring 310 and the water seepage ring 320 is a movable connection, and the water seepage ring 320 can move away from and approach the connecting ring 310 along the spiral slide rail 341; the lowest point of the bottom end of the spiral slide rail 341 is on the same horizontal plane as the central hole 332, the inner diameter of the spiral slide rail 341 is greater than the outer diameter of the connecting ring 310, and the outer diameter of the spiral slide rail 341 is less than the inner diameter of the container 400; one end of the first slider 343 close to the water seepage ring 320 is fixed on the water seepage ring 320, and the other end away from the water seepage ring 320 is placed on the spiral slide rail 341. The height of the first slider 343 is two-thirds of the axial distance between the center lines of the cross-sections of two adjacent turns on the spiral slide rail 341; the size of the second slider 344 is the same as that of the first slider 343, and the second slider 344 is fixed on the other side of the water seepage ring 320 relative to the position of the first slider 343; the position of the first slider 343 is higher than that of the second slider 344, and the height difference is one-half of the axial distance between the center lines of the cross-sections of two adjacent turns on the spiral slide rail 341, so that the first slider 343 and the second slider 344 can just slide up and down along the spiral slide rail 341; the bottom end of the stop block 342 is fixedly connected to the bottom end of the spiral slide rail 341, and the top end of the stop block 342 protrudes upward, so that the second slider 344 can stop when it slides to the bottom end of the spiral slide rail 341.

[0090] Further, the spiral direction of the spiral slide rail 341 from top to bottom is opposite to the direction in which the stirring ring assembly 260 blows air towards the liquid surface. That is, if the stirring ring assembly 260 blows air towards the liquid surface in a clockwise direction, the spiral direction of the spiral slide rail 341 from top to bottom is counterclockwise; if the stirring ring assembly 260 blows air towards the liquid surface in a counterclockwise direction, the spiral direction of the spiral slide rail 341 from top to bottom is clockwise; so that the stirring ring assembly 260 blowing air towards the liquid surface will not cause the bottom shell 330 to be blown and shaken.

[0091] As Figures 7 to 10As shown, the annular cavity 350 is integrally annular and is fixed on the inner wall of the bottom of the bottom shell 330. The outer diameter of the annular cavity 350 is smaller than that of the bottom shell 330, the inner diameter of the annular cavity 350 is larger than the radius of the central hole 332, the centers of the annular cavity 350 and the central hole 332 are located on the same vertical line, the middle part of the annular cavity 350 bulges towards the center of the bottom shell 330, and the inside of the annular cavity 350 is hollow; the bottom shell 330 further includes a rubber ring 333; the rubber ring 333 is integrally annular, the rubber ring 333 is located on the bottom shell 330 wrapped by the annular cavity 350, the inner ring of the rubber ring 333 is placed on the bottom shell 330, and the outer ring is fixedly connected to the bottom shell 330, separating the bottom shell 330 into two parts, and these two parts are fixed by the annular cavity 350; on the inner ring of the rubber ring 333, the side close to the stopper 331 bulges towards the center of the circle, and the thickness of the rubber ring 333 is greater than that of the bottom shell 330; the main body of the connecting pipe 360 is rod-shaped, the bottom end is fixed on the outer ring of the annular cavity 350, and the other end extends vertically upward along the inner wall of the bottom shell 330 to the junction of the water seepage ring 320 and the bottom shell 330. The inside of the connecting pipe 360 is through and the inside of the connecting pipe 360 is communicated with the inside of the annular cavity 350. The side of the connecting pipe 360 close to the inner wall of the bottom shell 330 is fixedly connected to the bottom shell 330; a communication port 361 is opened at the top end of the connecting pipe 360, and the communication port 361 is circular, so that gas or liquid can enter the connecting pipe 360 through the communication port 361.

[0092] As Figure 11 and Figure 12 As shown, a raised block 410 and an air intake assembly 420 are provided on the container 400; the raised block 410 is rectangular in shape and is the part where the bottom surface of the container 400 bulges towards the inside of the container 400; the air intake assembly 420 includes an intake pipe 421 and a piercing needle 422; the piercing needle 422 vertically passes through the raised block 410 and extends into the container 400. The piercing needle 422 is located directly below the rubber ring 333, and the length of the piercing needle 422 extending out of the raised block 410 is greater than the maximum vertical distance between the rubber ring 333 and the central hole 332; the piercing needle 422 is a through cylinder, the diameter of the cross-sectional circle of the piercing needle 422 is 1 cm, and the top end of the piercing needle 422 is needle-shaped, so that the piercing needle 422 can better pass through the rubber ring 333; the inside of the intake pipe 421 is communicated with the inside of the piercing needle 422, the intake pipe 421 is embedded in the raised block 410, and the end away from the piercing needle 422 is connected to the air pump assembly 240; in the air pump assembly 240, in addition to the air pump that supplies air to the stirring ring assembly 260, there is also an air pump that supplies air to the intake pipe 421, and the two air pumps do not interfere with each other.

[0093] Preferably, the position of the rubber ring 333 on the bottom shell 330 is close to the inner ring position of the annular cavity 350.

[0094] Further, the number of the connecting pipes 360 is two, and the two connecting pipes 360 are symmetrically arranged;

[0095] The radius of the connecting port 361 is half of the diameter of the penetrating needle 422; the width of the rubber ring 333 is twice the diameter of the penetrating needle 422.

[0096] During the experiment, it was found that when the secondary stirring device 300 was installed on the stirring ring assembly 260 and had not started stirring, since the water seepage ring 320 was above the liquid level of the solution in the container 400, the annular cavity 350 was filled with air inside. As a result, the buoyancy of the bottom shell 330 and the water seepage ring 320 from the solution was greater than their own gravity, causing the upper end of the water seepage ring 320 to abut against the lower end of the connecting ring 310; when the stirring ring assembly 260 blew air into the solution in the container 400, the solution would be blown into a vortex shape. At this time, a part of the solution would enter the connecting pipe 360 through the connecting port 361, and then flow into the annular cavity 350 along the connecting pipe 360. The solution would slowly flow into the annular cavity 350. At this time, the buoyancy of the bottom shell 330 and the water seepage ring 320 from the solution would slowly decrease. When this buoyancy was less than the gravity of the bottom shell 330 and the water seepage ring 320, the bottom shell 330 and the water seepage ring 320 would slide down along the spiral slide rail 341; when the bottom shell 330 and the water seepage ring 320 slid to the bottommost end, the penetrating needle 422 would penetrate through the rubber ring 333 and extend into the annular cavity 350. At this time, the corresponding air pump was turned on to inflate the annular cavity 350 through the air inlet pipe 421 and the penetrating needle 422. Under the extrusion of the gas, the solution in the annular cavity 350 would be discharged from the annular cavity 350 through the connecting pipe 360 and the connecting port 361. At this time, the buoyancy of the bottom shell 330 and the water seepage ring 320 from the solution would slowly increase. When the sum of the gravity of the bottom shell 330 and the water seepage ring 320 and the friction force between the penetrating needle 422 and the rubber ring 333 was less than this buoyancy, the annular cavity 350 would rise along the spiral slide rail 341; when the annular cavity 350 slid down or rose along the spiral slide rail 341, it would drive the upper layer solution and the lower layer solution in the container 400 to circulate and exchange.

[0097] The technical solutions in the embodiments of the present application at least have the following technical effects or advantages:

[0098] When stirring and mixing the mixed solution in the container 400, the secondary stirring device 300 can move up and down in the solution, enabling the upper layer solution and the lower layer solution in the container 400 to circulate and exchange, and the dissolution and mixing effect between the upper layer solution and the lower layer solution in the container 400 is better.

[0099] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A preparation device for sapindus saponin flavone nanomicelles, comprising a main stirring device (200), and the main stirring device (200) comprises a stirring ring assembly (260); characterized in that, It further includes a secondary stirring device (300); The secondary stirring device (300) can be fixed to the stirring ring assembly (260) by means of threaded connection; The secondary stirring device (300) includes a connecting ring (310), a water seepage ring (320) and a bottom shell (330); The connecting ring (310) is annular, located at the upper end of the secondary stirring device (300), and the outer diameter of the connecting ring (310) is smaller than the inner diameter of the container (400); The water seepage ring (320) is annular in shape, and the inner diameter and outer diameter of the water seepage ring (320) are the same as the inner diameter and outer diameter of the connecting ring (310); A number of through holes are evenly distributed on the water seepage ring (320); The bottom shell (330) is an overall hollow cylinder with an open top, and the bottom surface bulges downward into an arch shape. The upper end opening of the bottom shell (330) is fixedly connected to the lower end of the water seepage ring (320), and the inner diameter and outer diameter of the upper end opening of the bottom shell (330) are the same as the inner diameter and outer diameter of the water seepage ring (320); A central hole (332) is opened at the bottom of the bottom shell (330) for the exchange and circulation of the solution inside and outside the bottom shell (330); The bottom shell (330) also includes a rubber ring (333); The secondary stirring device (300) further includes a sliding assembly (340), an annular cavity (350) and a communicating pipe (360); The sliding assembly (340) includes a spiral slide rail (341), a stop block (342), a first slider (343) and a second slider (344); The spiral slide rail (341) is sleeved outside the secondary stirring device (300), and the top end of the spiral slide rail (341) is fixed on the connecting ring (310) and extends vertically downward in a spiral shape; The connection mode between the connecting ring (310) and the water seepage ring (320) is a movable connection, and the water seepage ring (320) can move away from and approach the connecting ring (310) along the spiral slide rail (341); A raised block (410) and an air inlet assembly (420) are provided on the container (400); The raised block (410) is rectangular in shape and is a part where the bottom surface of the container (400) protrudes into the interior of the container (400); The air inlet assembly (420) includes an air inlet pipe (421) and a penetration needle (422); The penetration needle (422) vertically penetrates through the raised block (410) and extends into the container (400). The penetration needle (422) is located directly below the rubber ring (333), and the length of the penetration needle (422) extending out of the raised block (410) is greater than the maximum vertical distance between the rubber ring (333) and the central hole (332); The penetration needle (422) is a through cylinder, the diameter of the cross-sectional circle of the penetration needle (422) is 1 cm, and the top end of the penetration needle (422) is needle-shaped, so that the penetration needle (422) can better penetrate through the rubber ring (333); The inside of the air inlet pipe (421) is connected to the inside of the penetration needle (422). The air inlet pipe (421) is embedded in the raised block (410), and the end away from the penetration needle (422) is connected to the air pump assembly (240); In addition to the air pump that conveys air to the stirring ring assembly (260) inside the air pump assembly (240), there is also an air pump that conveys air to the intake pipe (421), and the two air pumps do not interfere with each other.

2. The preparation device of sophora spine flavone nanomicelles according to claim 1, characterized in that, The outer surface of the lower end of the stirring ring housing (261) of the stirring ring assembly (260) is provided with an external thread. The spiral direction of the external thread on the outer surface of the stirring ring housing (261) from top to bottom is opposite to the direction in which the stirring ring assembly (260) blows air towards the liquid surface. The connecting ring (310) is provided with an internal thread that meshes with the external thread on the stirring ring housing (261), so that the secondary stirring device (300) can be fixedly connected to the stirring ring assembly (260).

3. The preparation device of sophora spina flavone nanomicelles according to claim 1, wherein A number of stoppers (331) are evenly arranged on the inner wall of the bottom shell (330). The stopper (331) is a cuboid. One end of the stopper (331) is fixed on the inner wall of the bottom shell (330), and the other end extends towards the center of the bottom shell (330) for making the dissolution and mixing of the solution inside the bottom shell (330) more sufficient.

4. The preparation device of sophora spina flavone nanomicelles according to claim 2, characterized in that, One end of the first slider (343) close to the water seepage ring (320) is fixed on the water seepage ring (320), and the other end away from the water seepage ring (320) is placed on the spiral slide rail (341). The height of the first slider (343) is two-thirds of the axial distance between the center lines of adjacent two turns of the cross-section of the spiral slide rail (341). The size of the second slider (344) is the same as that of the first slider (343), and the second slider (344) is fixed on the other side of the water seepage ring (320) relative to the position of the first slider (343). The position of the first slider (343) is higher than that of the second slider (344), and the height difference is one-half of the axial distance between the center lines of adjacent two turns of the cross-section of the spiral slide rail (341), so that the first slider (343) and the second slider (344) can just slide up and down along the spiral slide rail (341). The bottom end of the stop block (342) is fixedly connected to the bottom end of the spiral slide rail (341), and the top end of the stop block (342) protrudes upwards so that the second slider (344) can stop when it slides to the bottom end of the spiral slide rail (341). The spiral direction of the spiral slide rail (341) from top to bottom is opposite to the direction in which the stirring ring assembly (260) blows air towards the liquid surface.

5. The preparation device of sophora spina flavone nanomicelles according to claim 4, characterized in that, The lowest point of the bottom end of the spiral slide rail (341) is on the same horizontal plane as the central hole (332). The inner diameter of the spiral slide rail (341) is greater than the outer diameter of the connecting ring (310), and the outer diameter of the spiral slide rail (341) is less than the inner diameter of the container (400).

6. The preparation device of sophora spina flavone nanomicelles according to claim 5, characterized in that, The annular cavity (350) is integrally circular ring-shaped and is fixed on the inner wall of the bottom of the bottom shell (330). The outer diameter of the annular cavity (350) is less than the outer diameter of the bottom shell (330). The inner diameter of the annular cavity (350) is greater than the radius of the central hole (332). The center of the annular cavity (350) and the center of the central hole (332) are on the same vertical line. The middle part of the annular cavity (350) protrudes towards the center of the bottom shell (330), and the inside of the annular cavity (350) is hollow. The rubber ring (333) is in the shape of a circular ring as a whole. The rubber ring (333) is located on the bottom shell (330) wrapped by the annular cavity (350). The inner ring of the rubber ring (333) is placed on the bottom shell (330), and the outer ring is fixedly connected to the bottom shell (330), so as to divide the bottom shell (330) into two parts, and the two parts are fixed by the annular cavity (350); A side of the inner ring of the rubber ring (333) close to the stopper (331) protrudes toward the center of the circle, and the thickness of the rubber ring (333) is greater than the thickness of the bottom shell (330).

7. The preparation device of sophora spine flavone nanomicelles according to claim 6, characterized in that, The main body of the connecting pipe (360) is rod-shaped, with the bottom end fixed on the outer ring of the annular cavity (350), and the other end extending vertically upward along the inner wall of the bottom shell (330) to the junction of the water seepage ring (320) and the bottom shell (330); The interior of the connecting tube (360) is connected and the interior of the connecting tube (360) is connected to the interior of the annular cavity (350); the side surface of the connecting tube (360) close to the inner wall of the bottom shell (330) is fixedly connected to the bottom shell (330); The top end of the connecting tube (360) is provided with a connecting opening (361), and the connecting opening (361) is circular, so that gas or liquid can enter the connecting tube (360) through the connecting opening (361).

8. The preparation device of the sophora spina flavone nanomicelles according to claim 7, wherein, The number of the connecting pipes (360) is two, and the two connecting pipes (360) are symmetrically arranged; The radius of the communication port (361) is half the diameter of the penetration needle (422); The width of the rubber ring (333) is twice the diameter of the penetration needle (422).

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