A multi-pipette pipettor and method of pipetting
By designing a multi-channel pipette, an adjustable holder and squeezing block are used to achieve efficient transfer of liquid samples, solving the problem of cumbersome operation in existing technologies. This method is suitable for the detection of chemical components in tobacco samples.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-10-28
- Publication Date
- 2026-03-27
AI Technical Summary
Existing technologies cannot simultaneously transfer multiple liquid experimental samples from a large container to a small container, resulting in cumbersome, time-consuming, and error-prone operations, especially in the detection of chemical components in tobacco samples.
Design a multi-channel pipette including a base, a support, a squeezing device, a flexible tube, and a rigid tube. It achieves negative pressure extraction and squeezing operations through an adjustable fixing frame and squeezing block to transfer liquid samples from a large container to a small container.
It enables easy transfer of various liquid experimental samples, improves operational efficiency, and is suitable for the detection of chemical components in tobacco samples.
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Figure CN115591595B_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of chemical composition detection of tobacco samples, and more particularly to a multi-pipeline pipette and a pipetting method thereof. BACKGROUND
[0002] In the chemical composition detection of tobacco samples, a small amount of liquid experimental sample is usually transferred from a large container (such as a conical flask, a digestion tube, etc.) to a small container (such as a chromatographic flask, etc.) for on-machine detection. If multiple samples are to be taken and injected simultaneously and separately, a row gun is generally used for pipetting, but the size and distance of the sample container and the injection container must be the same. The row gun cannot take a small amount of liquid experimental sample from a row of large containers and transfer it to a row of small containers. Therefore, in actual operation, a pipette or a syringe is used to pipette each sample one by one. However, this has the problems of complicated operation, large consumption of manpower, long time consumption, and easy pipetting errors.
[0003] The prior art discloses an automatic device for pretreatment of metabolite detection, comprising a base, a reagent rack for placing pretreatment reagents, a pipetting device for transferring reagents, the pipetting device being provided with a pipetting module and a pipetting gun, a moving guide rail platform for supporting the pipetting device, a host computer for controlling the operation of the pipetting module, the reagent rack being arranged in the base, the reagent rack being provided with a plurality of reagent grooves equidistantly distributed from each other, the base being provided with a gun head storage groove for placing the pipetting gun, and the base being provided with a reaction plate storage position for placing a 96-hole reaction plate. In this scheme, the host computer controls the operation of the pipetting module, the pipetting gun is used to take samples from the reagent grooves at the same time, and the samples are dropped into the 96-hole reaction plate to complete pipetting. However, in this scheme, the pipetting guns are relatively fixed, the size and distance of the sample container and the injection container must be the same, and when used for the chemical composition detection of tobacco samples, multiple liquid experimental samples cannot be taken out from large containers and transferred to small containers at the same time. SUMMARY
[0004] The present application aims to overcome the shortcomings of the prior art and provide a multi-pipeline pipette and a pipetting method thereof, which can simultaneously take multiple liquid experimental samples from large containers and transfer them to small containers, and is suitable for pipetting in the chemical composition detection of tobacco samples.
[0005] To solve the above technical problems, the technical scheme adopted by the present application is as follows:
[0006] A multi-channel pipette is provided, comprising a base, a support, a squeezing device, several tubular tubes, several rigid tubes, a first tray for evenly spaced large containers, and a second tray for evenly spaced small containers. The first and second trays are detachably mounted on the top of the base, and the support is fixed to the top of the base. The squeezing device includes a fixing frame, a first squeezing block, a second squeezing block, and several drops with cavities. The fixing frame is detachably connected to the support via a first connecting assembly. The fixing frame has evenly spaced first through holes. One end of each of the tubular tubes passes through the first through holes and is connected to one of the drops. The other end of each tubular tube is connected to one end of a rigid tube. The other end of the rigid tube can extend into a large container on the first tray or be positioned directly above a small container on the second tray. The first and second squeezing blocks are hinged to the top of both sides of the fixing frame. The first and second squeezing blocks can cooperate to squeeze the drops, creating negative pressure in the cavity of the drops.
[0007] The multi-channel pipette of the present invention has a fixing frame detachably connected to the support via a first connecting assembly. In use, the first connecting assembly is first adjusted to adjust the height of the fixing frame to accommodate subsequent solution extraction. A first tray is placed on top of the base, and several large containers are evenly spaced on the first tray. The tubing is adjustable; by pulling several tubing tubes, the ends of several rigid tubes can be easily inserted below the liquid surface in the large containers as needed. Simultaneously, the first and second squeezing blocks are flipped to engage and squeeze several drops. Then, the first and second squeezing blocks are released, releasing the drops. The internal cavity generates negative pressure, causing several rigid tubes to draw solutions from several large containers. The rigid tubes are then removed from the large containers, the first tray is removed, and the second tray is placed on top of the base. Several small containers are evenly spaced on the second tray. The first connecting assembly can be adjusted again to adjust the height of the fixing frame so that the ends of the rigid tubes are directly above the small containers. Simultaneously, the first and second squeezing blocks are flipped, causing them to work together to squeeze the droppers, squeezing the drawn solutions into the small containers. The first and second squeezing blocks are then released, completing the multi-channel pipetting. This multi-channel pipette of the present invention can transfer various liquid experimental samples from large containers to small containers, and is suitable for pipetting in the chemical analysis of tobacco samples. It is easy to operate.
[0008] Furthermore, the base has a first groove on its top, and the first tray can be positioned and installed in the first groove.
[0009] Furthermore, the top of the first tray is provided with a third groove at even intervals for positioning and placing large containers.
[0010] Further, the base top is provided with a second groove, and the second groove is located in the first groove, the distance between the bottom surface of the second groove and the base top is greater than the distance between the bottom surface of the first groove and the base top.
[0011] Further, the second tray top is uniformly spaced and provided with fourth grooves for positioning and placing small containers.
[0012] Further, the first sliding block is further connected with the support through a second connecting assembly, the first sliding block is provided with a plurality of second through holes which are uniformly spaced, a plurality of the soft tubes are respectively arranged in the second through holes, and a plurality of the second through holes are respectively located above a plurality of small containers on the second tray top.
[0013] Further, the second sliding block is further connected with the support through a third connecting assembly, the second sliding block is provided with a plurality of third through holes which are uniformly spaced, a plurality of the soft tubes are respectively arranged in the third through holes, and a plurality of the third through holes are respectively located above a plurality of large containers on the first tray top, and the first sliding block is located between the fixed frame and the second sliding block.
[0014] Further, the first connecting assembly comprises a first connecting rod, a first open ring and a first bolt, the first open ring is arranged on the outer periphery of the support, the first bolt is threadedly connected with the first open ring, the first bolt can abut against the support to press the support and the first open ring tightly, and the two ends of the first connecting rod are respectively connected with the first open ring and the fixed frame.
[0015] The application further provides a pipetting method applied to the multi-pipeline pipettor, comprising the following steps:
[0016] S1: placing the first tray on the base top, and uniformly spacing a plurality of large containers on the first tray;
[0017] S2: pulling a plurality of soft tubes, and respectively inserting the ends of a plurality of hard tubes below the liquid level in the large containers;
[0018] S3: simultaneously turning over the first and second squeezing blocks, squeezing a plurality of the dripping heads by the first and second squeezing blocks, and simultaneously releasing the first and second squeezing blocks to respectively draw solutions in a plurality of the large containers by a plurality of the hard tubes;
[0019] S4: drawing the hard tubes out of the large containers, moving away the first tray, placing the second tray on the base top, uniformly spacing a plurality of small containers on the second tray, and respectively locating the ends of a plurality of the hard tubes above a plurality of the small containers;
[0020] S5: simultaneously flip the first extrusion block and the second extrusion block, so that the first extrusion block and the second extrusion block cooperate to extrude the plurality of drips, and extrude the solution drawn in step S3 into the plurality of small containers respectively, and simultaneously release the first extrusion block and the second extrusion block, to complete the multi-pipeline pipetting.
[0021] The pipetting method of the application, after the multi-pipeline pipettor is installed, places the first tray on the top of the base, places the plurality of large containers on the first tray in uniform intervals, adjusts the plurality of hoses, pulls the plurality of hoses, and inserts the ends of the plurality of hard tubes into the liquid surface of the large containers below as needed, simultaneously flips the first extrusion block and the second extrusion block, so that the first extrusion block and the second extrusion block cooperate to extrude the plurality of drips, simultaneously releases the first extrusion block and the second extrusion block, so that the cavity in the drip generates negative pressure, and the plurality of hard tubes draw the solution in the plurality of large containers respectively, then pulls the hard tubes out of the large containers, removes the first tray, places the second tray on the top of the base, places the plurality of small containers on the second tray in uniform intervals, then simultaneously flips the first extrusion block and the second extrusion block, so that the first extrusion block and the second extrusion block cooperate to extrude the plurality of drips, and extrude the drawn solution into the plurality of small containers respectively, and simultaneously releases the first extrusion block and the second extrusion block, to complete the multi-pipeline pipetting. The multi-pipeline pipettor of the application can take out and transfer a plurality of liquid experimental samples from large containers to small containers, is suitable for pipetting when detecting the chemical cost of tobacco samples, is easy to operate, and improves the efficiency.
[0022] Preferably, in step S4, in order to prevent the pipeline formed by the connection of the hose and the hard tube from being too long, the first connecting assembly is adjusted, and the fixing frame is moved upward along the support.
[0023] Compared with the background art, the multi-pipeline pipettor and the pipetting method thereof of the application have the following beneficial effects:
[0024] The multi-pipeline pipettor of the application can take out and transfer a plurality of liquid experimental samples from large containers to small containers, is suitable for pipetting when detecting the chemical cost of tobacco samples, is easy to operate, and improves the efficiency. BRIEF DESCRIPTION OF DRAWINGS
[0025] Figure 1 FIG. 1 is a structure schematic diagram of the multi-pipeline pipettor in the first perspective view in the embodiment of the application;
[0026] Figure 2 FIG. 2 is a structure schematic diagram of the multi-pipeline pipettor in the second perspective view in the embodiment of the application;
[0027] Figure 3 FIG. 3 is a connection schematic diagram of the extrusion device and the first connecting assembly in the embodiment of the application;
[0028] Figure 4 FIG. 4 is a structure schematic diagram of the extrusion device in the embodiment of the application;
[0029] Figure 5 Fig. 1 is a structural schematic diagram of a base in an embodiment of the present application;
[0030] Figure 6 Fig. 2 is a structural schematic diagram of a first tray in an embodiment of the present application;
[0031] Figure 7 Fig. 3 is a structural schematic diagram of a multi-pipeline pipettor in a pipetting state in an embodiment of the present application.
[0032] In the drawings: 1 - base; 101 - first groove; 102 - second groove; 2 - first tray; 201 - third groove; 202 - large container; 3 - second tray; 301 - small container; 4 - extrusion device; 401 - fixed frame; 4011 - first through hole; 402 - first extrusion block; 403 - second extrusion block; 404 - dripper; 5 - hose; 6 - hard pipe; 7 - first sliding block; 701 - second through hole; 8 - second sliding block; 9 - first connecting assembly; 901 - first open ring; 902 - first connecting rod; 903 - first bolt; 10 - second connecting assembly; 11 - third connecting assembly; 12 - support. DETAILED DESCRIPTION
[0033] The present application will be further described below in conjunction with specific embodiments. The drawings are only used for exemplary description, and the representation is only a schematic diagram, not a physical diagram, and should not be understood as a limitation of the present patent; in order to better illustrate the embodiments of the present application, some components in the drawings may be omitted, enlarged or reduced, and do not represent the actual product size; for those skilled in the art, it is understandable that some well-known structures in the drawings and their descriptions may be omitted.
[0034] The same or similar reference numerals in the drawings of the embodiments of the present application correspond to the same or similar components; in the description of the present application, it should be understood that if the terms "upper", "lower", "left", "right" and the like indicate the orientation or positional relationship shown in the drawings, they are only used for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, therefore the terms describing the positional relationship in the drawings are only used for exemplary description, and should not be understood as a limitation of the present patent, for those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.
[0035] Embodiment one
[0036] A multi-pipeline pipettor, such as Figures 1 to 4 , Figure 7As shown, it comprises a base 1, a support 12, an extrusion device 4, a plurality of hoses 5, a plurality of hard tubes 6, a first tray 2 for placing large containers 202 at uniform intervals and a second tray 3 for placing small containers 301 at uniform intervals, the first tray 2 and the second tray 3 are detachably installed on the top of the base 1, and the support 12 is fixed on the top of the base 1; the extrusion device 4 comprises a fixing frame 401, a first extrusion block 402, a second extrusion block 403 and a plurality of drippers 404 with cavities, the fixing frame 401 is detachably connected with the support 12 through a first connecting assembly 9, the fixing frame 401 is provided with a plurality of first through holes 4011 distributed at uniform intervals, one end of each of the plurality of hoses 5 is connected with one of the plurality of drippers 404 by penetrating the first through holes 4011 respectively, the other end of the hose 5 is connected with one end of the hard tube 6, the other end of the hard tube 6 can be inserted into the large container 202 on the first tray 2 or the other end of the hard tube 6 can be located directly above the small container 301 on the second tray 3, the first extrusion block 402 and the second extrusion block 403 are respectively hinged to the top of the two sides of the fixing frame 401, and the first extrusion block 402 and the second extrusion block 403 can cooperate to extrude the dripper 404 to generate negative pressure in the cavity of the dripper 404.
[0037] The multi-channel pipette described above, the fixing frame 401 is detachably connected with the support 12 through the first connecting assembly 9, in use, first adjust the first connecting assembly 9 to adjust the height of the fixing frame 401 to adapt to the subsequent solution extraction, place the first tray 2 on the top of the base 1, place a plurality of large containers 202 at uniform intervals on the first tray 2, the hose 5 is adjustable, pull a plurality of hoses 5, and then respectively insert the ends of a plurality of hard tubes 6 below the liquid level in the large containers 202 as needed, at the same time, turn over the first extrusion block 402 and the second extrusion block 403, so that the first extrusion block 402 and the second extrusion block 403 cooperate to extrude a plurality of drippers 404, then loosen the first extrusion block 402 and the second extrusion block 403, negative pressure is generated in the cavity of the dripper 404, a plurality of hard tubes 6 respectively extract the solution in a plurality of large containers 202, then pull out the hard tube 6 from the large container 202, remove the first tray 2, place the second tray 3 on the top of the base 1, place a plurality of small containers 301 at uniform intervals on the second tray 3, adjust the first connecting assembly 9 again to adjust the height of the fixing frame 401, so that the ends of a plurality of hard tubes 6 are respectively located directly above a plurality of small containers 301, at the same time, turn over the first extrusion block 402 and the second extrusion block 403, so that the first extrusion block 402 and the second extrusion block 403 cooperate to extrude a plurality of drippers 404, and then respectively extrude the extracted solution into a plurality of small containers 301, at the same time, loosen the first extrusion block 402 and the second extrusion block 403, to complete multi-channel pipetting. The multi-channel pipette of the embodiment can take out a plurality of liquid experimental samples from the large containers 202 and transfer them to the small containers 301, and is suitable for pipetting in chemical cost detection of tobacco samples, and is simple to operate.
[0038] AsFigure 5 As shown in the figure, the first tray 2 is positioned and installed in the first groove 101. Figure 6 As shown in the figure, the first tray 2 is positioned and installed in the first groove 101.
[0039] As shown in the figure, the first tray 2 is positioned and installed in the first groove 101. Figure 5 As shown in the figure, the first tray 2 is positioned and installed in the first groove 101.
[0040] As shown in the figure, the first tray 2 is positioned and installed in the first groove 101. Figure 2 Figure 3 As shown in the figure, the first tray 2 is positioned and installed in the first groove 101.
[0041] As shown in the figure, the first tray 2 is positioned and installed in the first groove 101. Figure 3 As shown, the first pressing block 402 is provided with one, the second pressing block 403 is provided with two, and the two second pressing blocks 403 are located on the same side of the fixed frame 401, a gap is left between the two second pressing blocks 403, and the first connecting rod 902 can pass through the gap and be connected with the fixed frame 401. When the number of liquid experimental samples that need to be simultaneously pipetted is reduced, only the first pressing block 402 and one of the second pressing blocks 403 can be used for liquid taking and pipetting.
[0042] Embodiment two
[0043] This embodiment is similar to embodiment one, and the difference is that, as shown in Figure 1 、 Figure 2 , it further comprises a second sliding block 8 connected with the bracket 12 through a third connecting assembly 11, the second sliding block 8 is provided with a plurality of third through holes uniformly arranged, and the ends of the plurality of soft tubes 5 away from the dripper 404 are respectively arranged in the third through holes, and the third through holes are respectively located directly above the large containers 202 on the top of the first tray 2. Specifically, the third connecting assembly 11 comprises a third connecting rod, a third split ring and a third bolt, the third split ring is arranged on the outer periphery of the bracket 12, the third bolt is threadedly connected with the third split ring, the third bolt can abut against the bracket 12 to press the bracket 12 and the third split ring tightly, and the two ends of the third connecting rod are respectively connected with the third split ring and the second sliding block 8. In implementation, the first tray 2 on which a plurality of large containers 202 are placed is positioned and installed on the first groove 101 on the top of the base 1, and the plurality of large containers 202 respectively contain liquid experimental samples, the third bolt is loosened, the second sliding block 8 is pulled down by hand, the second sliding block 8 moves downward along the soft tubes 5 and the bracket 12 to abut against the top openings of the large containers 202, at the same time, the hard tubes 6 are inserted into the large containers 202, so that the ends of the hard tubes 6 are inserted below the liquid surface in the large containers 202, the second sliding block 8 and the third through holes thereon make the distance between the hard tubes 6 match the distance between the large containers 202 on the first tray 2, the third bolt is tightened to fix the second sliding block 8, then the first pressing block 402 and the second pressing block 403 are turned up at the same time, the first pressing block 402 and the second pressing block 403 jointly press the dripper 404, then the first pressing block 402 and the second pressing block 403 are loosened at the same time, the liquid experimental samples in the large containers 202 are simultaneously drawn up by the hard tubes 6, then the third bolt is loosened and taken out, the third split ring is separated from the bracket 12, and the third connecting assembly 11 and the second sliding block 8 are disassembled. It should be noted that different first trays 2 can be designed according to actual needs to place different numbers of different large containers 202, and different second sliding blocks 8 can be designed according to different first trays 2 to ensure that the hard tubes 6 can match the large containers 202 when the liquid experimental samples are drawn.
[0044] As shown in Figure 1 、 Figure 2 and Figure 7As shown, the first sliding block 7 is slidably connected to the support 12 through the second connecting assembly 10, the first sliding block 7 is provided with a plurality of second through holes arranged at equal intervals, a plurality of soft tubes 5 are respectively arranged in the second through holes, and the plurality of second through holes are respectively located above the plurality of small containers 301 on the top of the second tray 3, and the first sliding block 7 is located between the fixing frame 401 and the second sliding block 8. Specifically, the second connecting assembly 10 includes a second connecting rod, a second open ring and a second bolt, the second open ring is arranged on the outer periphery of the support 12, the second bolt is threadedly connected with the second open ring, the second bolt can abut against the support 12 to press the support 12 and the second open ring tightly, and the two ends of the second connecting rod are respectively connected with the second open ring and the first sliding block 7. In implementation, the second tray 3 on which a plurality of small containers 301 are placed is positioned and installed on the second groove 102 on the top of the base 1, the second bolt is loosened, the first sliding block 7 is pulled down by hand, the first sliding block 7 moves downward along the soft tubes 5 and the support 12 to make the plurality of second through holes clamp the hard tubes 6, the first sliding block 7 and the second through holes thereon, so that the spacing between the hard tubes 6 matches the distance between the small containers 301 on the second tray 3, at this time, the ends of the hard tubes 6 are located above the openings on the top of the small containers 301, the second bolt is tightened to fix the first sliding block 7, then the first pressing block 402 and the second pressing block 403 are turned up at the same time, the first pressing block 402 and the second pressing block 403 press the dripper 404 together, the extracted liquid experimental sample is squeezed into the small containers 301, the first pressing block 402 and the second pressing block 403 are loosened and restored, the multi-pipeline pipetting is completed, and the effect of simultaneously transferring a small amount of a plurality of liquid experimental samples from a large container 202 to a plurality of small containers 301 is realized. It should be noted that different second trays 3 can be designed according to actual needs to place different numbers of different small containers 301, and different first sliding blocks 7 can be designed according to different second trays 3 to ensure that each hard tube 6 can match each small container 301 when the liquid experimental sample is squeezed out.
[0045] Embodiment three
[0046] The embodiment is a pipetting method applied to the multi-pipeline pipettor of the embodiment one or the embodiment two, including the following steps:
[0047] S1: placing the first tray 2 on the top of the base 1 and placing a plurality of large containers 202 on the first tray 2 at equal intervals;
[0048] S2: pulling a plurality of soft tubes 5 and inserting the ends of a plurality of hard tubes 6 below the liquid level in the large containers 202; S3: turning the first pressing block 402 and the second pressing block 403 at the same time, so that the first pressing block 402 and the second pressing block 403 press a plurality of drippers 404 together, loosening the first pressing block 402 and the second pressing block 403 at the same time, and a plurality of hard tubes 6 respectively extract solutions in a plurality of large containers 202;
[0049] S4: pulling the hard tubes 6 out of the large containers 202, removing the first tray 2, placing the second tray 3 on the top of the base 1, and placing the small containers 301 on the second tray 3 in uniform intervals, with the ends of the hard tubes 6 being located directly above the small containers 301.
[0050] S5: simultaneously turning over the first pressing block 402 and the second pressing block 403 to make the first pressing block 402 and the second pressing block 403 cooperate to press the several drippers 404, so as to extrude the solution extracted in step S3 into the small containers 301, and simultaneously releasing the first pressing block 402 and the second pressing block 403, thus completing the multi-pipeline pipetting.
[0051] The above pipetting method, after the multi-pipeline pipettor is installed, places the first tray 2 on the top of the base 1, places the several large containers 202 on the first tray 2 in uniform intervals, adjusts the soft tubes 5, pulls the soft tubes 5, and conveniently inserts the ends of the hard tubes 6 into the liquid surface in the large containers 202 according to the needs, simultaneously turns over the first pressing block 402 and the second pressing block 403 to make the first pressing block 402 and the second pressing block 403 cooperate to press the several drippers 404, simultaneously releases the first pressing block 402 and the second pressing block 403, generates negative pressure in the cavities in the drippers 404, so as to extract the solution in the several large containers 202 through the several hard tubes 6, then pulls the hard tubes 6 out of the large containers 202, removes the first tray 2, places the second tray 3 on the top of the base 1, places the small containers 301 on the second tray 3 in uniform intervals, adjusts the first connecting assembly 9 again, moves the fixing frame 401 along the support 12, so that the ends of the hard tubes 6 are located directly above the small containers 301, simultaneously turns over the first pressing block 402 and the second pressing block 403 to make the first pressing block 402 and the second pressing block 403 cooperate to press the several drippers 404, so as to extrude the extracted solution into the small containers 301, and simultaneously releases the first pressing block 402 and the second pressing block 403, thus completing the multi-pipeline pipetting. The multi-pipeline pipettor can take out various liquid experimental samples from the large containers 202 and transfer them to the small containers 301, and is suitable for pipetting when the chemical cost of the tobacco sample is detected, and is simple to operate.
[0052] In step S1, the first tray 2 is positioned and placed in the first groove 101, and the plurality of large containers 202 are positioned and placed in the third groove 201, so as to ensure that the plurality of large containers 202 are arranged in a row in an orderly manner.
[0053] In step S2, the specific process is: setting the second sliding block 8 connected with the support 12 through the third connecting assembly 11, loosening the third bolt, and then pulling down the second sliding block 8 by hand, so that the second sliding block 8 moves downward along the hose 5 and the support 12 to abut against the top opening of the large container 202, and meanwhile, the hard pipes 6 are inserted into the large container 202, so that the ends of the hard pipes 6 are inserted below the liquid level in the large container 202, and then the third bolt is tightened to fix the second sliding block 8.
[0054] In step S4, in order to ensure that the ends of the hard pipes 6 can be located directly above the small containers 301, the first sliding block 7 connected with the support 12 through the second connecting assembly 10 is set, the plurality of second through holes on the first sliding block 7 correspond to the openings of the small containers 301 respectively, the second bolt is loosened, and then the first sliding block 7 is pulled down by hand until the plurality of second through holes are clamped on the hard pipes 6.
[0055] In the specific content of the above specific embodiments, any non-contradictory combination of technical features can be made, and in order to make the description simple, all possible combinations of the above technical features are not described, however, as long as the combination of the technical features does not exist, it should be considered as the scope of the present application.
[0056] Obviously, the above embodiments of the present application are only examples for clearly illustrating the present application, and are not intended to limit the implementation modes of the present application. Based on the above description, other different forms of changes or variations can be made by those skilled in the art. Here, all the implementation modes are not required or can not be exhausted. Any modification, equivalent replacement and improvement within the spirit and principle of the present application should be included in the protection scope of the claims of the present application.
Claims
1. A multi-channel pipettor characterized in that, The utility model provides a kind of container extruding device, including base (1), support (12), extrusion device (4), several hoses (5), several hard tubes (6), first tray (2) for placing large container (202) in uniform interval and second tray (3) for placing small container (301) in uniform interval, the first tray (2) and the second tray (3) are detachably installed on the top of the base (1), and the support (12) is fixed on the top of the base (1);The extrusion device (4) includes fixed frame (401), first extrusion block (402), second extrusion block (403) and several drip heads (404) with cavity, the fixed frame (401) is detachably connected with the support (12) by first connecting assembly (9), the fixed frame (401) is provided with first through hole (4011) distributed in uniform interval, one end of several hoses (5) is connected with several drip heads (404) one by one by the first through hole (4011), the other end of the hose (5) is connected with one end of the hard tube (6), the other end of the hard tube (6) is inserted into large container (202) on the first tray (2) or the other end of the hard tube (6) is located directly above small container (301) on the second tray (3), the first extrusion block (402), second extrusion block (403) are respectively hinged with the top of both sides of the fixed frame (401), and the first extrusion block (402), second extrusion block (403) cooperate extrusion drip head (404) to make the cavity in the drip head (404) produce negative pressure; It further includes first sliding block (7) slidably connected with the support (12) by second connecting assembly (10), the first sliding block (7) is provided with second through hole arranged in uniform interval, and several hoses (5) are respectively arranged in the second through hole, and several second through holes are respectively located directly above several small containers (301); It further includes second sliding block (8) slidably connected with the support (12) by third connecting assembly (11), the second sliding block (8) is provided with third through hole arranged in uniform interval, and one end of several hoses (5) away from the drip head (404) is respectively arranged in the third through hole, and the third through hole is respectively located directly above large container (202) on the top of the first tray (2), and the first sliding block (7) is located between the fixed frame (401) and the second sliding block (8).
2. The multi-channel pipettor of claim 1, wherein, The top of the base (1) is provided with first groove (101), and the first tray (2) is positioned and installed in the first groove (101).
3. The multi-channel pipettor of claim 2, wherein, The top of the first tray (2) is provided with third groove (201) for positioning and placing large container (202) in uniform interval.
4. The multi-channel pipettor of claim 2, wherein, The top of the base (1) is provided with second groove (102), and the second groove (102) is located in the first groove (101), and the distance between the bottom surface of the second groove (102) and the top of the base (1) is greater than the distance between the bottom surface of the first groove (101) and the top of the base (1).
5. The multi-channel pipettor of claim 4, wherein, The second tray (3) is uniformly spaced on the top and provided with fourth grooves for positioning and placing small containers (301).
6. The multi-channel pipettor of any of claims 1-5, wherein, The first connecting assembly (9) comprises a first connecting rod (902), a first open ring (901) and a first bolt (903), the first open ring (901) is arranged on the outer periphery of the support (12), the first bolt (903) is threadedly connected with the first open ring (901), the first bolt (903) abuts against the support (12) to press the support (12) and the first open ring (901) tightly, and the two ends of the first connecting rod (902) are connected with the first open ring (901) and the fixing frame (401) respectively.
7. A pipetting method applied to the multi-channel pipettor of any one of claims 1-6, characterized in that, The method comprises the following steps: S1: placing the first tray (2) on the top of the base (1), and placing a plurality of large containers (202) uniformly and spacedly on the first tray (2); S2: pulling a plurality of hoses (5), and inserting the ends of a plurality of hard pipes (6) below the liquid level in the large containers (202) respectively; S3: simultaneously turning over the first extrusion block (402) and the second extrusion block (403), so that the first extrusion block (402) and the second extrusion block (403) extrude a plurality of drippers (404) cooperatively, and simultaneously releasing the first extrusion block (402) and the second extrusion block (403), so that a plurality of hard pipes (6) draw solutions in a plurality of large containers (202) respectively; S4: pulling the hard pipes (6) out of the large containers (202), removing the first tray (2), placing the second tray (3) on the top of the base (1), placing a plurality of small containers (301) uniformly and spacedly on the second tray (3), and locating the ends of a plurality of hard pipes (6) above a plurality of small containers (301) respectively; S5: simultaneously turning over the first extrusion block (402) and the second extrusion block (403), so that the first extrusion block (402) and the second extrusion block (403) extrude a plurality of drippers (404) cooperatively, and extrude the solutions drawn in step S3 into a plurality of small containers (301) respectively, and simultaneously releasing the first extrusion block (402) and the second extrusion block (403), thereby completing multi-pipeline pipetting.
8. The pipetting method according to claim 7, characterized in that In step S4, in order to prevent the pipeline formed by the connection of the hose (5) and the hard pipe (6) from being too long, the first connecting assembly (9) is adjusted, and the fixing frame (401) is moved upward along the support (12).
Citation Information
Patent Citations
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