An automated nucleic acid extraction and sample preparation device

Abstract

This invention provides an automated nucleic acid extraction and sample preparation device, including a frame; a working platform mounted on the frame; a sample application platform capable of moving along the Y direction; sample application deep-well plate placement positions arranged sequentially along the X direction on the sample application platform; multiple deep-well plate placement positions also arranged along the X direction on the sample application platform; a gripping and transfer module, a sample application module, and a nucleic acid extraction module mounted on the frame; the gripping and transfer module and the sample application module are movable relative to the frame along the X, Y, and Z directions, and the nucleic acid extraction module is movable relative to the frame along the X and Z directions; the gripping and transfer module moves a deep-well plate containing sample liquid to the nucleic acid extraction placement position; the nucleic acid extraction module extracts nucleic acid from the sample liquid in the deep-well plate; the gripping and transfer module grips the nucleic acid-extracted deep-well plate to the sample application deep-well plate placement position; and the sample application module extracts a predetermined amount of sample liquid from the deep-well plate to the sample application plate, forming a nucleic acid test sample.

Description

Technical Field

[0001] This invention belongs to the field of automated nucleic acid detection technology, and in particular relates to an automated nucleic acid extraction and sample preparation device. Background Technology

[0002] In existing nucleic acid testing, a conveyor line is typically set up, with a pipetting structure, a nucleic acid extraction structure, and a sample application module arranged sequentially on both sides of the conveyor line. The pipetting structure extracts sample liquid from the sample tube and moves it into a deep-well plate. A robotic arm places the deep-well plate on the conveyor line and moves it to the nucleic acid extraction structure. The robotic arm then picks up the deep-well plate and moves it to the nucleic acid extraction structure, where it extracts nucleic acid from the sample liquid in the deep-well plate. After nucleic acid extraction, the robotic arm moves the deep-well plate back to the conveyor line, and after being conveyed, it moves to the sample application module. The robotic arm picks up the deep-well plate again and transfers it to the sample application module, where the sample application module extracts the nucleic acid-extracted sample liquid from the deep-well plate and applies it to the sample application plate, thus preparing a nucleic acid test sample.

[0003] The aforementioned deep-well plate requires the assistance of a robotic arm and a conveyor line for transfer between various structures. Similarly, the nucleic acid extraction structure requires the transport of all solvents, consumables, and waste materials via a conveyor line, with the robotic arm delivering them to their designated locations. Likewise, the spotting structure requires the transport of consumables and waste materials via a conveyor line, with the robotic arm delivering them to their designated locations. These structures occupy a large area, are assembled together during use, and cannot be moved as a whole; disassembly is required for movement, hindering rapid deployment. Summary of the Invention

[0004] To address the shortcomings of existing technologies, the present invention aims to provide an automated nucleic acid extraction and sample preparation device that can be quickly transferred as a whole and put into use immediately. It can be used immediately after being assembled before leaving the factory, without the need for on-site assembly and debugging, thus reducing on-site workload.

[0005] To achieve this objective, the present invention adopts the following technical solution:

[0006] This invention provides an automated nucleic acid extraction and sample preparation device, comprising:

[0007] frame;

[0008] A work platform, which is mounted on the rack;

[0009] A sampling platform is disposed on the working platform, and a sampling Y-axis platform component is disposed between the platform and the working platform. The sampling Y-axis platform component can drive the sampling platform to move along the Y-axis.

[0010] The sampling platform is equipped with a sampling deep-hole plate placement position;

[0011] The spotting platform is also provided with multiple deep well plate placement positions along the X direction. The deep well plate placement positions are all located behind the spotting deep well plate placement positions. One of the deep well plate placement positions is a nucleic acid extraction placement position, which is configured to place the deep well plate containing sample liquid. The other deep well plate placement positions are configured to place consumables required for nucleic acid extraction.

[0012] The gripping and transfer module, the sample spotting module, and the nucleic acid extraction module are all mounted on the rack and are located above the sample spotting platform. The gripping and transfer module and the sample spotting module can move relative to the rack in the X, Y, and Z directions, and move synchronously in the X direction. The nucleic acid extraction module can move relative to the rack in the X and Z directions.

[0013] The gripping and transfer module moves the deep-well plate containing the sample liquid to the nucleic acid extraction placement position;

[0014] The nucleic acid extraction module is located on the rear side of the plurality of deep well plate placement positions and is used to extract nucleic acids from the sample liquid in the deep well plate at the nucleic acid extraction placement position.

[0015] The clamping and transfer module clamps the deep well plate after nucleic acid extraction to the spotting deep well plate placement position, and the spotting module is used to extract a predetermined amount of sample liquid from the deep well plate to the spotting plate to form a nucleic acid test sample.

[0016] Preferably, the clamping and transferring module includes:

[0017] Transfer X-axis moving components, which are mounted on the rack;

[0018] A Y-axis moving component is disposed on the X-axis moving component;

[0019] A Z-axis moving component is disposed on the Y-axis moving component, and the Y-axis moving component drives the Z-axis moving component to move along the Y-axis.

[0020] A transfer clamping assembly is disposed on the transfer Z-axis moving assembly. The transfer Z-axis moving assembly can drive the transfer clamping assembly to clamp the deep hole plate and drive the deep hole plate to rotate.

[0021] Preferably, the Y-axis transfer component includes:

[0022] A Y-axis fixed plate is transferred and disposed on the X-axis moving assembly;

[0023] A Y-axis transfer drive assembly is disposed on the Y-axis transfer fixing plate;

[0024] Two sets of second belt pulley assemblies, each set of second belt pulley assemblies is connected to the output end of one set of the Y-axis transfer drive assembly, and the Z-axis transfer movement assembly is connected to the second belt pulley assembly;

[0025] A Y-axis sliding component is disposed on the Y-axis fixed plate and located between the two sets of second belt pulley assemblies. The Z-axis moving component and the dotting module are both connected to the Y-axis sliding component and can slide independently along the Y direction.

[0026] Preferably, the Y-axis sliding component includes:

[0027] A Y-axis sliding member is provided on a Y-axis fixed plate.

[0028] Two sets of Y-axis sliding blocks are slidably mounted on the Y-axis sliding member. The Z-axis moving component is connected to one set of Y-axis sliding blocks and is also connected to one set of second belt pulley assemblies.

[0029] The sampling module is connected to another set of Y-axis sliding blocks, and the sampling module is also connected to another set of second belt pulley assemblies.

[0030] Preferably, the transfer clamping assembly includes:

[0031] A Z-axis guide assembly and a transfer electric gripper connected thereto, the Z-axis guide assembly being connected to the Z-axis moving assembly, the Z-axis guide assembly providing guidance for the Z-axis movement of the transfer electric gripper;

[0032] The output end of the transfer electric gripper is provided with a rotating clamping block, the rotating clamping block is provided with a clamping groove, and a clamping slider is provided in the clamping groove for sliding cooperation.

[0033] And clamping arms, each of the clamping sliders is connected to a set of clamping arms, the two sets of clamping arms can move toward or relative to each other in the same direction, and the clamping arms are configured to clamp the two sides of the deep hole plate.

[0034] Preferably, the clamping arm includes:

[0035] The clamping arm body has one end connected to the clamping slider;

[0036] A clamping movable block is connected to the front end of the clamping arm body, and a wear-resistant pad is provided between the clamping arm body and the clamping movable block;

[0037] A connecting rotating component is provided, which is axially arranged along the Z direction and passes through and connects the clamping arm body, the wear-resistant pad and the clamping movable block. The clamping movable block can rotate relative to the clamping arm body along the Z direction by a first preset angle.

[0038] A second elastic element is disposed between the clamping movable block and the connecting rotating element, and the axial direction of the second elastic element is perpendicular to the side wall of the deep hole plate clamped by the clamping arm body.

[0039] Preferably, the spotting Y-axis platform component includes:

[0040] The first linear motor is mounted on the working platform.

[0041] A first Y-axis linear slide for sampling is connected to the output end of the first linear motor for sampling, and the sampling platform is disposed on the first Y-axis linear slide for sampling.

[0042] A first sampling guide component is disposed on the working platform and is arranged in parallel with the first sampling Y-axis linear slide. The sampling platform is connected to the first sampling guide component. The first sampling linear motor can drive the sampling platform located on the first sampling Y-axis linear slide to move along the first sampling guide component.

[0043] Preferably, the nucleic acid extraction module includes:

[0044] A nucleic acid extraction X-axis driving component is disposed on the working platform and located behind the deep well plate placement position;

[0045] A nucleic acid extraction rack is disposed on the nucleic acid extraction X-direction driving component, which is capable of driving the nucleic acid extraction rack to move along the X-direction.

[0046] A nucleic acid extraction Z-axis drive component is disposed on the nucleic acid extraction rack;

[0047] A nucleic acid extraction mounting assembly is connected to the nucleic acid extraction Z-axis drive assembly, and the nucleic acid extraction mounting assembly can move synchronously relative to the nucleic acid extraction frame along the Z-axis. The nucleic acid extraction mounting assembly can grip or release the magnetic rod sleeve.

[0048] A nucleic acid extraction component is disposed directly above the nucleic acid extraction mounting component and connected to the output end of the nucleic acid extraction mounting component. The nucleic acid extraction mounting component can drive the nucleic acid extraction component to move relative to the nucleic acid extraction frame along the Z direction, so that the nucleic acid extraction component can be installed or removed from the magnetic rod sleeve.

[0049] Preferably, the nucleic acid extraction and installation component includes:

[0050] A first mounting bracket is connected to the nucleic acid extraction Z-axis driving assembly and slidably connected to the nucleic acid extraction frame. The first mounting bracket is provided with a second opening for the magnetic rod of the nucleic acid extraction assembly to pass through.

[0051] A first mounting drive component is disposed on the first mounting frame, and its output end is connected to the nucleic acid extraction component. The first mounting drive component can drive the nucleic acid extraction component to move along the Z direction.

[0052] A clamping rod assembly, which passes through the first mounting bracket and is located on both sides of the second opening;

[0053] A rotation drive assembly is disposed on the first mounting bracket and connected to the clamping rod assembly. The rotation drive assembly drives the clamping rod assembly to rotate along its own axis to clamp or release the magnetic rod sleeve at the second opening.

[0054] Preferably, the rotation drive assembly includes:

[0055] A pressure element is disposed on the nucleic acid extraction component and is capable of moving synchronously with the nucleic acid extraction component along the Z direction;

[0056] A tension spring, one end of which is connected to the first mounting bracket and the other end of which is connected to the clamping rod assembly, wherein the axial direction of the tension spring forms a second preset angle with the X direction;

[0057] A pressure contact is provided on the clamping rod assembly. When the first mounting drive assembly drives the nucleic acid extraction assembly to move downward along Z, the pressure contact can slide along the pressure member and press against the locking part of the pressure member. The tension spring can drive the clamping rod assembly to rotate to release the magnetic rod sleeve.

[0058] Compared with the prior art, the present invention has the following beneficial effects:

[0059] In this invention, a Y-axis sampling platform component is set on the working platform. This component drives the sampling platform on it to move along the Y-axis, thereby causing the sampling deep-hole plate placement positions and multiple deep-hole plate placement positions to move synchronously along the Y-axis. Simultaneously, in conjunction with the clamping and transfer module and the sampling module, they can move along the X, Y, and Z axes, enabling the sampling deep-hole plate placement positions on the sampling platform to quickly reach their relative positions with the sampling module and the clamping and transfer module, thereby reducing working time and improving work efficiency.

[0060] Furthermore, the spotting module and the gripping and transfer module move synchronously along the X-axis. This reduces the space occupied by the X-axis structure of each module, resulting in a compact, small, and flexible structure. On the other hand, the synchronous movement of the two modules, after reaching the vicinity of the spotting deep-well plate placement position, allows the gripping and transfer module to place the deep-well plate gripped from the nucleic acid extraction module at the spotting deep-well plate location. Simultaneously, it grips the deep-well plate containing sample liquid, ready to move again to the nucleic acid extraction placement position. Meanwhile, the spotting module moves along the Y and Z axes to adjust its position and extract the deep-well plate from the spotting deep-well plate placement position.

[0061] At the same time, the sampling platform moves each placement position along the Y direction to facilitate manual replenishment of consumables and removal of used consumables.

[0062] The gripping and transfer module can move the deep-well plate containing the sample liquid it grips to the nucleic acid extraction placement position. Through the movement of the sampling platform along the Y direction, and in coordination with the movement of the nucleic acid extraction module along the X and Z directions, the nucleic acid extraction module and each deep-well plate placement position on the sampling platform can be quickly moved to a relatively suitable position to start the nucleic acid extraction work, thereby reducing the movement time and improving work efficiency. Attached Figure Description

[0063] Figure 1 This is a schematic diagram of the automated nucleic acid extraction and sample preparation device of the present invention from a first angle.

[0064] Figure 2 This is a second-angle structural schematic diagram of the automated nucleic acid extraction and sample preparation device of the present invention;

[0065] Figure 3 This is a schematic diagram of the sampling platform component and the placement positions of each deep hole plate in the present invention at the first angle.

[0066] Figure 4 This is a schematic diagram of the sampling platform component and the placement positions of each deep hole plate in the present invention from a second angle.

[0067] Figure 5 This is a schematic diagram of the transfer clamping module and the sampling module in this invention;

[0068] Figure 6 This is a schematic diagram of the structure of a portion of the transfer clamping assembly and the second scanning module in this invention;

[0069] Figure 7 This is a schematic diagram of the clamping arm in this invention;

[0070] Figure 8 This is a schematic diagram of the clamping arm in this invention (excluding the clamping arm body);

[0071] Figure 9 This is a schematic diagram of the nucleic acid extraction module in this invention from a first angle;

[0072] Figure 10 This is a schematic diagram of the nucleic acid extraction module in this invention from a second angle.

[0073] Figure 11 This is a schematic diagram of the nucleic acid extraction and installation component of the present invention from a first angle.

[0074] Figure 12 This is a schematic diagram of the nucleic acid extraction and installation component of the present invention from a second angle.

[0075] Figure 13 This is a schematic diagram of the nucleic acid extraction and installation component of the present invention from a third angle.

[0076] Among them, 1 is the rack; 100 is the work platform;

[0077] 6. Clamping and transferring module;

[0078] 61. Transfer Y-axis moving assembly; 611. Transfer Y-axis fixed plate; 612. Transfer Y-axis driving assembly; 613. Second belt and pulley assembly; 614. Transfer Y-axis sliding assembly; 6141. Transfer Y-axis sliding block; 6142. Transfer Y-axis sliding member;

[0079] 62. Transfer Z-axis movement component; 621. Transfer Z-axis drive component; 622. Lead screw and nut assembly;

[0080] 63. Transfer clamping assembly; 631. Transfer Z-direction guide assembly; 632. Transfer electric gripper; 633. Rotary clamping block; 6331. Clamping slide; 6332. Clamping slider; 634. Clamping arm; 6341. Clamping arm body; 6342. Clamping movable block; 6343. Connecting rotating component; 6344. Second elastic component; 6345. Wear-resistant pad;

[0081] 64. Second scanning module;

[0082] 7. Nucleic acid extraction module; 70. Second opening; 700. First deep-well plate placement position; 701. Second deep-well plate placement position; 702. Third deep-well plate placement position; 703. Fourth deep-well plate placement position; 704. Fifth deep-well plate placement position; 705. Nucleic acid extraction placement position;

[0083] 71. Nucleic acid extraction X-axis driving component;

[0084] 72. Nucleic acid extraction rack;

[0085] 73. Nucleic acid extraction Z-axis drive assembly; 730. Nucleic acid extraction motor; 732. Third belt and pulley assembly;

[0086] 74. Nucleic acid extraction installation assembly; 741. First mounting frame; 742. First installation drive assembly; 743. Clamping rod assembly; 7431. Rotating rod; 7432. Blocking component; 744. Rotation drive assembly; 7441. Pressing component; 7442. Tension spring; 7443. Pressing contact component;

[0087] 75. Nucleic acid extraction components;

[0088] 76. Anti-drip assembly; 761. Anti-drip drive assembly; 7611. First anti-drip drive motor; 7612. Gear; 7613. Rack; 762. Drip plate assembly; 7621. Drip connecting plate; 7622. Drip receiving plate;

[0089] 8. Sampling module; 81. Sampling Y-axis platform component; 811. Sampling first linear motor; 812. Sampling first Y-axis linear slide; 82. Sampling first guide component; 823. First slider; 824. First slide rail; 83. Sampling component;

[0090] 86. Sampling platform; 800. Sampling deep hole plate placement position; 801. Sampling plate placement position; 802. Sampling syringe placement position; 803. Sampling waste placement position;

[0091] 87. Spot Z-axis moving component. Detailed Implementation

[0092] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The components of the embodiments of the present invention described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0093] Therefore, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the invention without inventive effort are within the scope of protection of the invention.

[0094] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0095] In the description of this invention, it should be noted that the terms "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this invention is in use. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance. In the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0096] In the description of this invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0097] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0098] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0099] like Figures 1-13 As shown, this embodiment provides an automated nucleic acid extraction and sample preparation device, including a frame 1, a working platform 100, a sample application platform 86, a gripping and transfer module 6, a sample application module 8, and a nucleic acid extraction module 7. The working platform 100 is mounted on the frame 1, the sample application platform 86 is mounted on the working platform 100, and a sample application Y-direction platform component 81 is provided between the working platform 100 and the sample application platform 86. The sample application Y-direction platform component 81 can drive the sample application platform 86 to move along the Y direction.

[0100] The sample application platform 86 is provided with a sample application deep well plate placement position 800, a sample application plate placement position 801, a sample application needle placement position 802, and a sample waste placement position 803 arranged sequentially along the X direction. Multiple deep well plate placement positions are also provided along the X direction on the sample application deep well plate placement position 800. One of these deep well plate placement positions is a nucleic acid extraction placement position 705, configured to hold a deep well plate containing sample liquid. The other deep well plate placement positions are configured to hold consumables required for nucleic acid extraction.

[0101] The clamping and transfer module 6, the spotting module 8, and the nucleic acid extraction module 7 are all mounted on the frame 1 and are located above the spotting platform 86. The clamping and transfer module 6 and the spotting module 8 can move relative to the frame 1 in the X, Y, and Z directions, and move synchronously in the X direction. The nucleic acid extraction module 7 can move relative to the frame 1 in the X and Z directions.

[0102] The clamping and transfer module 6 moves the deep-well plate containing the sample liquid to the nucleic acid extraction placement position 705. The nucleic acid extraction module 7 is used to extract nucleic acids from the sample liquid in the deep-well plate at the nucleic acid extraction placement position 705.

[0103] The clamping and transfer module 6 clamps the deep well plate after nucleic acid extraction to the spotting deep well plate placement position 800. The spotting module 8 is used to extract a predetermined amount of sample liquid from the deep well plate to the spotting plate to form a nucleic acid test sample.

[0104] In this embodiment, a Y-axis sampling platform component 81 is provided on the working platform 100. The sampling platform 86 on the Y-axis component 81 is driven to move along the Y-axis, thereby causing the sampling deep hole plate placement position 800 and multiple deep hole plate placement positions on it to move synchronously along the Y-axis. At the same time, in conjunction with the clamping and transfer module 6 and the sampling module 8, they can move along the X, Y, and Z axes, so that the sampling deep hole plate placement position 800 on the sampling platform 86 can quickly reach the relative position with the sampling module 8 and the clamping and transfer module 6, thereby reducing working time and improving working efficiency.

[0105] In this embodiment, a deep hole plate placement position 800, a spotting plate placement position 801, a spotting needle placement position 802, and a spotting waste placement position 803 are sequentially arranged along the X direction on the spotting platform 86.

[0106] Furthermore, the spotting module 8 and the clamping and transfer module 6 move synchronously along the X-axis. This reduces the space occupied by the X-axis structure of each module, resulting in a compact, small, and flexible structure. Simultaneously, after the two modules move to the vicinity of the spotting deep-well plate placement position 800, the clamping and transfer module 6 places the deep-well plate clamped from the nucleic acid extraction module 7 at the spotting deep-well plate location, while simultaneously clamping the deep-well plate containing sample liquid to prepare for re-moving to the nucleic acid extraction placement position 705. Meanwhile, the spotting module 8 moves along the Y and Z axes to adjust its position and extract the deep-well plate from the spotting deep-well plate placement position 800.

[0107] At the same time, the sampling platform 86 drives each placement position on it to move along the Y direction, so that consumables can be manually replenished upwards and used consumables can be removed.

[0108] The clamping and transfer module 6 can move the deep well plate containing the sample liquid it clamps to the nucleic acid extraction placement position 705. Through the movement of the sampling platform along the Y direction, and in coordination with the movement of the nucleic acid extraction module 7 along the X and Z directions, the nucleic acid extraction module 7 and each deep well plate placement position on the sampling platform can quickly move to a relatively suitable position to start the nucleic acid extraction work, thereby reducing the movement time and improving work efficiency.

[0109] The specific structure of the Y-axis platform component 81 mentioned above includes:

[0110] The first linear motor 811 is used for sampling, and it is mounted on the work platform 100;

[0111] The first Y-axis linear slide 812 for sampling is connected to the output end of the first linear motor 811 for sampling, and the sampling platform 86 is disposed on the first Y-axis linear slide 812 for sampling.

[0112] The first sample guide component 82 is disposed on the working platform 100 and is arranged in parallel with the first sample Y-direction linear slide 812. The sample platform 86 is connected to the first sample guide component 82. The first sample linear motor 811 can drive the sample platform 86 located on the first sample Y-direction linear slide 812 to move along the first sample guide component 82.

[0113] When the above-mentioned sampling platform 86 moves along the Y direction, the first sampling guide component 82 provides auxiliary guidance for the sampling platform 86, so that when various consumables and materials are placed at each placement position on it, it can move smoothly along the Y direction.

[0114] Preferably, the first sample guide assembly 82 includes a first slide rail 824 disposed along the Y direction on the working platform 100, and a first slider 823 connected to the sample tube placement stage. The first slider 823 slides in cooperation with the first slide rail 824. By sliding the first slider 823 along the first slide rail 824, and cooperating with the first linear motor and the first Y-direction linear slide table to support and slide the sample tube placement stage, its structure is simple, does not require much space, and compared with two sets of the above-mentioned opening Y-direction feeding assemblies arranged in parallel, its cost is low and it is easy to install and maintain.

[0115] Preferably, regarding the specific structure of the clamping and transfer module 6 described above, the clamping and transfer module 6 includes a transfer X-axis moving component 61, a transfer Y-axis moving component 61, a transfer Z-axis moving component 62, and a transfer clamping component 63. The transfer Y-axis moving component 61 is disposed on the transfer X-axis moving component, and in this embodiment, the transfer X-axis moving component passes through the spacer and is disposed along the X-axis. The transfer Z-axis moving component 62 is disposed on the transfer Y-axis moving component 61, and the transfer Y-axis moving component 61 drives the transfer Z-axis moving component 62 to move along the Y-axis. The transfer clamping component 63 is disposed on the transfer Z-axis moving component 62, and the transfer Z-axis moving component 62 can drive the transfer clamping component 63 to clamp the deep hole plate and cause the deep hole plate to rotate.

[0116] In this embodiment, the Y-axis transfer component 61 and the Y-axis pipetting component are both mounted on the same X-axis transfer component, saving space in the overall machine. Furthermore, the Z-axis transfer component 62 can drive the transfer clamping component 63 to move along the Z-axis, clamping the deep-well plate and rotating the clamped deep-well plate. In this embodiment, the transfer clamping component 63 can pass through the first opening of the spacer, clamp the deep-well plate at the pipetting module, and move to the nucleic acid extraction module 7 to extract nucleic acid. After nucleic acid extraction, the transfer clamping component 63 transfers the deep-well plate to the sample application module 8 to extract a predetermined amount of liquid onto the sample application plate.

[0117] The aforementioned clamping and transfer module 6 operates within the space on the right side of the spacer and can move along the X, Y, and Z directions to reciprocate the deep well plate between the pipetting module, the nucleic acid extraction module 7, and the sample spotting module 8.

[0118] Preferably, the Y-axis moving component 61 includes a Y-axis fixed plate 611, a Y-axis driving component 612, two sets of second belt pulley assemblies 613, and a Y-axis sliding component 614. The Y-axis fixed plate 611 is disposed on the Y-axis moving component, the Y-axis driving component 612 is disposed on the Y-axis fixed plate 611, each set of second belt pulley assemblies 613 is connected to the output end of one set of Y-axis driving components 612, and the Z-axis moving component 62 is connected to the second belt pulley assemblies 613. The Y-axis sliding component 614 is disposed on the Y-axis fixed plate 611 and located between the two sets of second belt pulley assemblies 613. The Z-axis moving component 62 and the sampling module 8 are both connected to the Y-axis sliding component 614 and can slide independently along the Y-axis.

[0119] In this implementation, the spotting module 8 and the Z-axis moving component 62 are connected by a Y-axis sliding component 614. The two move synchronously along the X-axis. When the transfer clamping component 63 moves the deep well plate after the nucleic acid extraction module 7 extracts nucleic acid to the spotting point for spotting, the spotting module 8 moves synchronously to the spotting point. Then, the position can be adjusted along the Y and Z axes. The synchronous movement of the spotting module 8 and the Z-axis moving component 62 saves time and improves work efficiency.

[0120] The specific structure of the aforementioned Z-axis transfer moving component 62 includes a Z-axis transfer drive component 621 and a lead screw and nut assembly 622. The Z-axis transfer drive component 621 is connected to the first belt and pulley assembly and also to the Y-axis sliding component. The lead screw and nut assembly 622 is connected to the output end of the Z-axis transfer drive component 621. The Z-axis transfer drive component 621 drives the lead screw and nut assembly 622 to move, thereby causing the transfer clamping component 63 to move along the Z-axis. In this embodiment, the Z-axis transfer drive component 621 drives the lead screw and nut assembly 622 to move, thereby causing the transfer clamping component 63 to move along the Z-axis. This structure is simple, small in size, and the overall structure of the Z-axis transfer moving component 62 is lightweight and flexible in movement.

[0121] In this embodiment, the Z-axis transfer drive assembly 621 includes a fifth motor, the output of which is connected to the lead screw and nut assembly 622. The output of the fifth motor is directly connected to the lead screw and nut assembly 622. The fifth motor drives the lead screw and nut assembly 622 and drives the transfer clamping assembly 63 to be set along the Z-axis. This structure is simple, low-cost, occupies little space, and is easy to maintain.

[0122] Specifically, the Y-axis sliding assembly 614 includes a Y-axis sliding member 6142 disposed on the Y-axis fixed plate 611, and two sets of Y-axis sliding blocks 6141 slidably disposed on the Y-axis sliding member 6142. The Z-axis moving assembly 62 is connected to one set of Y-axis sliding blocks 6141, and is also connected to one set of second belt pulley assemblies 613. The sampling module 8 is connected to the other set of Y-axis sliding blocks 6141, and is also connected to the other set of second belt pulley assemblies 613.

[0123] Preferably, the aforementioned transfer clamping assembly 63 includes a transfer Z-axis guide assembly 631, a transfer electric gripper 632 connected thereto, and clamping arms 634. The transfer Z-axis guide assembly 631 is connected to the transfer Z-axis moving assembly 62, and provides guidance for the transfer electric gripper 632 to move along the Z-axis. A rotating clamping block 633 is provided at the output end of the transfer electric gripper 632. A clamping groove 6331 is provided on the rotating clamping block 633, and a clamping slider 6332 is provided within the clamping groove 6331 for sliding cooperation. Each clamping slider 6332 is connected to a set of clamping arms 634. The two sets of clamping arms 634 can move towards or relative to each other in the same direction. The clamping arms 634 are configured to clamp both sides of the deep hole plate.

[0124] When the aforementioned Z-axis moving component 62 drives the electric gripper 632 to move along the Z-axis, the Z-axis guiding component 631 guides the electric gripper 632, ensuring the stability of its movement. Simultaneously, the Z-axis guiding component 631 is located between the Z-axis moving component 62 and the electric gripper 632. The transfer clamping component 63 and the Z-axis moving component 62 have a compact structure and occupy little space, thus reducing the overall size of the machine.

[0125] Preferably, the clamping arm 634 includes a clamping arm body 6341, a clamping movable block 6342, a connecting rotating member 6343, and a second elastic member 6344. One end of the clamping arm body 6341 is connected to the clamping slider 6332, the clamping movable block 6342 is connected to the front end of the clamping arm body 6341, and a wear-resistant pad 6345 is provided between the clamping arm body 6341 and the clamping movable block 6342. The connecting rotating member 6343 is axially arranged along the Z-direction and passes through the clamping arm body 6341, the wear-resistant pad 6345, and the clamping movable block 6342. The clamping movable block 6342 can rotate relative to the clamping arm body 6341 along the Z-direction by a first preset angle. The second elastic member 6344 is disposed between the clamping movable block 6342 and the connecting rotating member 6343, and the axial direction of the second elastic member 6344 is perpendicular to the sidewall of the deep hole plate clamped by the clamping arm body 6341.

[0126] In this embodiment, when the clamping arm body 6341 clamps the side wall of the deep hole plate, the side wall of the deep hole plate is not a vertical plane due to dimensional errors, and the vertical distance between the two side walls of different deep hole plates is different, that is, there are errors in the width dimension of the deep hole plate. Therefore, when both the clamping arm body 6341 and the clamping movable block 6342 are in contact with the deep hole plate, the clamping arm body 6341 and the clamping movable block 6342 are rotated at a small angle through the connecting rotating member 6343. A second elastic member 6344 is provided between the two, and the relative position between the two can be adaptively rotated and adjusted to adapt to the contact and fit of the side wall of the deep hole plate, and stably clamp the deep hole plate.

[0127] Preferably, in this embodiment, a set of second elastic members 6344 is provided on both sides of the connecting rotating member 6343. Providing two sets of second elastic members 6344 ensures that after relative rotation between the clamping movable block 6342 and the clamping arm body 6341, the two sets of second elastic members 6344 can maintain the balance of the clamping movable block 6342, allowing the clamping movable block 6342 to fully adhere to the deep hole plate.

[0128] Preferably, the second elastic element 6344 is a spring.

[0129] Preferably, the aforementioned clamping and transfer module 6 further includes a second scanning module 64 disposed on the clamping arm 634. The second scanning module 64 is configured to scan the information of the deep-well plate clamped by the transfer clamping assembly 63. In this embodiment, the second scanning module 64 is configured to scan the information of the deep-well plate clamped by the transfer clamping assembly 63, so as to facilitate scanning and recording the information of the deep-well plate clamped from the pipetting module. At the same time, it scans the information of the deep-well plate after nucleic acid extraction from the nucleic acid extraction module 7, so as to compare and record the information of the deep-well plate and prevent errors.

[0130] Preferably, the sampling module 8 includes a sampling Y-axis platform component 81, which is disposed on the working platform 100. A sampling platform 86 is disposed on the sampling Y-axis platform component 81. A sampling deep hole plate placement position 800, a sampling plate placement position 801, a sampling needle placement position 802, and a sampling waste placement position 803 are sequentially disposed on the sampling platform 86 along the X-axis from the spacer plate. The sampling Y-axis platform component 81 can drive the sampling platform 86 to reciprocate along the Y-axis.

[0131] In this embodiment, the specific structure of the Y-axis platform component 81 is the same as that of the Y-axis feeding component with the cover opened, and will not be described again here.

[0132] The deep-well plate placement position 800 is used to place deep-well plates clamped from the pipetting assembly and returned to the same position from the nucleic acid extraction module 7. The deep-well plate placement position 801 is used to place unused deep-well plates, and the deep-well plate placement position 802 is used to place unused deep-well plates. The waste placement position 803 is used to place used deep-well plates.

[0133] The aforementioned Y-axis platform component 81 drives the spotting platform 86 to work along the Y-axis. The spotting platform 86, the aforementioned transfer clamping component 63, and the spotting module 8 all move synchronously along the Y-axis, so that the deep hole plate and spotting plate located on the spotting platform 86 can quickly be in a suitable position, so as to facilitate the work of the transfer clamping component 63 and the spotting module 8 and improve work efficiency.

[0134] In addition, the above-mentioned sampling platform 86 is located on the front side of the rack 1. The sampling platform 86 can move its various placement positions to the front door of the rack 1 so that the staff can replenish materials and remove used materials. That is, unused sampling plates and sampling needles are placed in the above-mentioned placement positions, and used sampling waste is removed.

[0135] Regarding the specific structure of the above-mentioned sampling module 8, it further includes a sampling Z-axis moving component 87 and a sampling component 83. The sampling Z-axis moving component 87 is disposed on the transfer Y-axis moving component 61 and is capable of moving along the Y-axis. The sampling component 83 is connected to the sampling Z-axis moving component 87, and the sampling Z-axis moving component 87 can drive the sampling component 83 to move along the Z-axis.

[0136] In this embodiment, the Z-axis moving component 87 is driven to move along the Z-axis, and in conjunction with its movement along the Y and X axes, a predetermined amount of sample liquid from the deep-well plate after nucleic acid extraction is transferred to the spotting plate. The spotting module 8 and the gripping and transfer module 6 move synchronously along the X-axis using the same pipetting X-axis moving component, improving work efficiency, reducing the structural setup of each module moving independently, reducing the overall structural volume, and lowering costs.

[0137] The dispensing component 83 is a standard dispensing structure available on the market and can be selected according to actual needs.

[0138] The specific structure of the Z-axis moving component 87 is the same as that of the Z-axis moving component 62 described above, and will not be repeated here.

[0139] The aforementioned nucleic acid extraction module 7 is located behind the clamping and transfer module 6 and the sample spotting module 8. All three are located on the same side of the spacer, making reasonable use of the front and rear space within the frame 1. The structure is compact and reduces the overall size of the machine.

[0140] Regarding the specific structure of the above-mentioned nucleic acid extraction module 7, it includes a plurality of deep well plate placement positions arranged sequentially along the X direction from the spacer on the sample placement platform 86. The deep well plate placement positions are located behind the sample deep well plate placement position 800. The deep well plate placement position at the position farthest from the spacer is configured to place the pipetted deep well plate clamped by the transfer clamping assembly 63 from the sample deep well plate placement position 800.

[0141] The aforementioned deep well plate placement positions include, sequentially from the spacer along the X direction, the first deep well plate placement position 700, the second deep well plate placement position 701, the third deep well plate placement position 702, the fourth deep well plate placement position 703, the fifth deep well plate placement position 704, and the nucleic acid extraction placement position 705.

[0142] The first deep-well plate placement position 700 to the fifth deep-well plate placement position 704 are the aforementioned nucleic acid extraction consumable positions, configured to hold different types of consumables or solvents required for nucleic acid extraction.

[0143] Furthermore, each of the aforementioned deep-well plate placement positions can be located on the same sampling platform 86 as the sample-dispensing deep-well plate placement position 800, sample-dispensing plate placement position 801, sample-dispensing needle placement position 802, and sample-dispensing waste placement position 803. All of these placement positions can move synchronously along the Y-axis to facilitate the loading and unloading of materials. Simultaneously, all of these placement positions move synchronously along the Y-axis and are arranged in two rows along the X-axis, reducing the space occupied in the horizontal plane. This rational layout satisfies both the operation of the transfer and clamping module 6 and the sample-dispensing module 8 on the front side, and the operation of the nucleic acid extraction module 7 on the rear side.

[0144] Regarding the specific structure of the aforementioned nucleic acid extraction module 7, the nucleic acid extraction module 7 further includes a nucleic acid extraction X-axis driving component 71, a nucleic acid extraction frame 72, a nucleic acid extraction Z-axis driving component 73, a nucleic acid extraction mounting component 74, and a nucleic acid extraction component 75. The nucleic acid extraction X-axis driving component 71 is mounted on the working platform 100 and located behind the deep well plate placement position. The nucleic acid extraction frame 72 is mounted on the nucleic acid extraction X-axis driving component 71, which drives the nucleic acid extraction frame 72 to move along the X-axis. The nucleic acid extraction Z-axis driving component 73 is mounted on the nucleic acid extraction frame 72. The nucleic acid extraction mounting component 74 is connected to the nucleic acid extraction Z-axis driving component 73 and can move synchronously with respect to the nucleic acid extraction frame 72 along the Z-axis. The nucleic acid extraction mounting component 74 can grip or release the magnetic rod sleeve. The nucleic acid extraction component 75 is positioned directly above the nucleic acid extraction mounting component 74 and connected to the output end of the nucleic acid extraction mounting component 74. The nucleic acid extraction mounting component 74 can drive the nucleic acid extraction component 75 to move relative to the nucleic acid extraction frame 72 in the Z direction, so that the nucleic acid extraction component 75 can be installed or removed from the magnetic rod sleeve.

[0145] In this embodiment, each of the above-mentioned deep well plate placement positions can move along the Y direction to directly below the nucleic acid extraction component 75. The nucleic acid extraction X-direction driving component 71 can drive the nucleic acid extraction component 75 to move along the X direction, and the nucleic acid extraction Z-direction driving component 73 can drive the nucleic acid extraction component 75 to move along the Z direction, so that the nucleic acid extraction component 75 moves into the deep well plate for nucleic acid extraction.

[0146] The aforementioned nucleic acid extraction mounting component 74 is located directly below the nucleic acid extraction component 75. It is used to clamp or detach the magnetic rod sleeve, allowing the nucleic acid extraction component 75 to insert into or detach from the magnetic rod sleeve. In this embodiment, the magnetic rod sleeve is placed at one of the aforementioned deep-well plate placement positions.

[0147] Preferably, the aforementioned nucleic acid extraction X-direction drive assembly 71 includes a nucleic acid extraction X-direction electric cylinder, which is disposed on the working platform 100 and located behind the sample spotting platform 86, and the nucleic acid extraction rack 72 is connected to the nucleic acid extraction X-direction electric cylinder. In this embodiment, the aforementioned nucleic acid extraction X-direction electric cylinder is a conventional electric cylinder, and its model can be selected according to actual needs, which is low in cost and easy to use.

[0148] Preferably, the above-mentioned nucleic acid extraction Z-direction drive assembly 73 includes a nucleic acid extraction motor 730 and a third belt pulley assembly 732. The nucleic acid extraction motor 730 is disposed on the nucleic acid extraction frame 72, the third belt pulley assembly 732 is disposed on the nucleic acid extraction frame 72, and the output end of the third belt pulley assembly 732 is connected to the nucleic acid extraction motor 730. The nucleic acid extraction mounting assembly 74 is connected to the third belt pulley assembly 732. The nucleic acid extraction motor 730 drives the third belt pulley to move and drives the nucleic acid extraction mounting assembly 74 to move along the Z direction.

[0149] The nucleic acid extraction mounting assembly 74 is driven to move along the Z-axis by a nucleic acid extraction motor 730 and a third belt and pulley assembly 732. This design is simple in structure, occupies little space, is compact, easy to replace in case of failure, and has low cost. In this embodiment, the third belt and pulley assembly 732 is located on one side of the nucleic acid extraction frame 72, and the nucleic acid extraction motor 730 is located at the bottom of the nucleic acid extraction frame 72, making full use of the space at the bottom of the nucleic acid extraction frame 72 and resulting in a more compact overall structure.

[0150] Preferably, the aforementioned nucleic acid extraction mounting assembly 74 includes a first mounting frame 741, a first mounting drive assembly 742, a clamping rod assembly 743, and a rotation drive assembly 744. The first mounting frame 741 is connected to the nucleic acid extraction Z-axis drive assembly 73 and slidably connected to the nucleic acid extraction frame 72. The first mounting frame 741 has a second opening 70 for the magnetic rod of the nucleic acid extraction assembly 75 to pass through. The first mounting drive assembly 742 is mounted on the first mounting frame 741, and its output end is connected to the nucleic acid extraction assembly 75. The first mounting drive assembly 742 can drive the nucleic acid extraction assembly 75 to move along the Z-axis. The clamping rod assembly 743 passes through the first mounting frame 741 and is located on both sides of the second opening 70. The rotation drive assembly 744 is mounted on the first mounting frame 741 and connected to the clamping rod assembly 743. The rotation drive assembly 744 drives the clamping rod assembly 743 to rotate along its own axis to clamp or release the magnetic rod sleeve at the second opening 70.

[0151] In this embodiment, the nucleic acid extraction Z-axis driving component 73 drives the first mounting frame 741 and its components to move downwards along the Z-axis to the placement position of the deep-hole plate containing the unused magnetic rod sleeve, located directly below it. After the nucleic acid extraction mounting component 74 continues to move downwards to its position, the first mounting driving component 742 drives the nucleic acid extraction component 75 to move downwards. At this time, the rotation driving component 744 drives the clamping rod component 743 to rotate, thereby clamping the unused magnetic rod sleeve, and the magnetic rod of the nucleic acid extraction component 75 is inserted into the magnetic rod sleeve. After the magnetic rod of the nucleic acid extraction component 75 is inserted into the magnetic rod sleeve, the nucleic acid extraction Z-axis driving component 73 drives the nucleic acid extraction component 75 and the nucleic acid mounting component to move upwards synchronously along the Z-axis. Then, driven by the nucleic acid extraction X-axis driving component 71, they move synchronously along the X-axis to the clamping and transfer module 6 to clamp the deep-hole plate at the nucleic acid extraction placement position 705 for nucleic acid extraction.

[0152] Preferably, the rotation drive assembly 744 includes a pressing member 441, a tension spring 7442, and a pressing contact member 7443. The pressing member 441 is disposed on the nucleic acid extraction assembly 75 and can move synchronously with the nucleic acid extraction assembly 75 along the Z-direction. One end of the tension spring 7442 is connected to the first mounting bracket 741, and the other end is connected to the clamping rod assembly 743. The axial direction of the tension spring 7442 forms a second preset angle with the X-direction. The pressing contact member 7443 is disposed on the clamping rod assembly 743. When the first mounting drive assembly 742 drives the nucleic acid extraction assembly 75 to move downwards along the Z-direction, the pressing contact member 7443 can slide along the pressing member 441 and press against the locking portion of the pressing member 441. The tension spring 7442 can drive the clamping rod assembly 743 to rotate, thereby releasing the magnetic rod sleeve.

[0153] In this embodiment, as the pressing contact 7443 moves downward along the Z direction, it slides along the pressing member 441 until it contacts the locking part of the pressing member 441. At this time, the tension spring 7442 is stretched. Since the axis of the tension spring 7442 forms a second preset angle with the X direction, after the pressing contact 7443 moves downward to press against the locking part, the tension spring 7442 is stretched, thereby driving the clamping rod assembly 743 to rotate to open, thereby releasing the used magnetic rod sleeve and placing it at one of the deep hole plate placement positions. The specific position can be selected from the first deep hole plate placement position 700 to the fifth deep hole plate placement position 704 according to actual needs.

[0154] Furthermore, after the clamping rod assembly 743 places the used magnetic rod sleeve, it moves to the deep-hole plate placement position for placing the unused magnetic rod sleeve. The first mounting drive assembly 742 drives the nucleic acid extraction assembly 75 downward. At this time, the aforementioned pressing contact 7443 disengages from the locking part of the pressing member 441 and begins to slide along the pressing member 441. The clamping rod assembly 743 rotates to clamp the unused magnetic rod sleeve, and the magnetic rod of the nucleic acid extraction assembly 75 is inserted into the magnetic rod sleeve. Afterward, the nucleic acid extraction mounting assembly 74 and the nucleic acid extraction assembly 75 rise synchronously in the Z direction and move in the X direction to the nucleic acid extraction placement position 705 located on the sample spotting platform 86, ready to extract the nucleic acid from the sample liquid inside.

[0155] In this embodiment, a tension spring 7442 is connected to the clamping rod assembly 743. The mutual pressing action between the contact member 7443 and the pressing member 441 drives the clamping rod assembly 743 to rotate via the tension spring 7442, thereby clamping or releasing the deep hole plate. This design is simple, low-cost, space-saving, and easy to maintain and replace. In other embodiments, the rotation drive assembly 744 may include a rotation motor, which is mounted on the first mounting bracket 741, and its output end is connected to the clamping rod assembly 743.

[0156] The clamping rod assembly 743 has the following specific structure: it includes a rotating rod 7431 and a stopper 7432. The rotating rod 7431 passes through the first mounting bracket 741 and is rotatable relative to the first mounting bracket 741 along its own axial direction. One end of the stopper 7432 is disposed on the rotating rod 7431, and the rotating rod 7431 can rotate with the rotating rod. The other end of the stopper 7432 can clamp the magnetic rod sleeve.

[0157] In this embodiment, the rotation of the rotating rod 7431 drives the rotation of the blocking member 7432. The blocking members 7432 on both sides clamp the magnetic rod sleeve. The structure is simple and occupies little space, greatly reducing the overall size of the machine. Preferably, each rotating rod 7431 is provided with two blocking members 7432 at a third preset distance along its axial direction to uniformly and stably clamp the magnetic rod sleeve.

[0158] Preferably, the nucleic acid extraction module 7 further includes an anti-drip component 76, which is disposed on the nucleic acid extraction rack 72. The anti-drip component 76 can be located directly below the nucleic acid extraction component 75 to catch liquid dripping from the magnetic rod sleeve. The anti-drip component 76 can also be moved to allow the nucleic acid extraction component 75 to operate. In this embodiment, the anti-drip component 76 is provided to ensure that after the nucleic acid extraction component 75 extracts nucleic acid in the deep well plate, the sample liquid adhering to the magnetic rod sleeve drips onto other structures, causing contamination and affecting normal operation.

[0159] The aforementioned anti-drip assembly 76 includes an anti-drip drive assembly 761 and two sets of oppositely arranged drip plate assemblies 762. The anti-drip drive assembly 761 is mounted on the nucleic acid extraction rack 72 and located above the nucleic acid extraction assembly 75. The two sets of drip plate assemblies 762 are located on both sides of the nucleic acid extraction assembly 75, and are connected to the output end of the anti-drip drive assembly 761. The anti-drip drive assembly can drive the two sets of drip plate assemblies 762 to move relative to each other to directly below the magnetic rod sleeve, or to move in opposite directions to either side of the magnetic rod sleeve. In this embodiment, the anti-drip drive assembly is positioned above the nucleic acid extraction assembly 75 to fully utilize the space above the nucleic acid extraction rack 72 without affecting the normal operation of other structures. The drip plate assemblies 762 are positioned on both sides of the nucleic acid extraction assembly 75, utilizing the space on both sides of the nucleic acid extraction assembly 75 without occupying the working space of other structures; the arrangement is reasonable and the overall structure is compact.

[0160] Preferably, the anti-drip drive assembly 761 includes a first anti-drip drive motor 7611, a gear 7612, and two sets of racks 7613. The first anti-drip drive motor 7611 is mounted on the nucleic acid extraction rack 72 and positioned above the nucleic acid extraction assembly 75. The gear 7612 is connected to the output end of the first anti-drip drive motor 7611. The two sets of racks 7613 are respectively disposed on both sides of the gear 7612 and mesh with it. The racks 7613 are positioned along the X-direction. The first anti-drip drive motor 7611 drives the gear 7612 to rotate, thereby causing the racks 7613 to move along the X-direction. Each drip plate assembly 762 is connected to one of the racks 7613, and the rack 7613 drives the drip plate to move along the X-direction.

[0161] In this embodiment, the structure of a first anti-drip drive motor 7611, gear 7612, and rack 7613 above the nucleic acid extraction component 75 is utilized, and drip plate components 762 are arranged on both sides of the nucleic acid extraction component 75. The structure is compact, simple, occupies little space, and is low in cost. In other embodiments, the anti-drip component 76 may also include a second anti-drip drive motor and a pull-out plate, wherein the second anti-drip drive motor is mounted on a first mounting bracket 741. The pull-out plate is slidably mounted on the first mounting bracket 741 and connected to the output end of the second anti-drip drive motor, and the pull-out plate can reciprocate along the Y direction.

[0162] The specific structure of the aforementioned dropper plate assembly 762 includes a dropper connecting plate 7621 and a dropper receiving plate 7622. One end of the dropper connecting plate 7621 is connected to the rack 7613. The other end of each dropper connecting plate 7621 is connected to the dropper receiving plate 7622, ​​and the two sets of dropper receiving plates 7622 can move relative to or away from each other. The structure of the dropper plate assembly 762 in this embodiment is simple, and the two dropper receiving plates 7622 are located on both sides of the nucleic acid extraction assembly 75 along the X direction, utilizing the space along the X direction to reduce the occupation of the space in the Z direction, thereby reducing the overall height of the machine and making the overall structure compact. In addition, after the nucleic acid extraction of the sample liquid in a batch of sample tubes 300 is completed, this structure facilitates the disassembly of the dropper receiving plate for disassembly, cleaning, and maintenance. The aforementioned nucleic acid extraction assembly 75 includes a magnetic rod mounting plate slidably connected to the nucleic acid extraction rack 72 and a magnetic rod assembly mounted on the magnetic rod mounting plate. The magnetic rod mounting plate is connected to the output end of the first mounting drive assembly 742. The magnetic rod assembly is an existing structure and can be selected according to actual needs.

[0163] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. An automated nucleic acid extraction and sample preparation device, characterized in that, include: Rack (1); A work platform (100) is mounted on the frame (1); A sampling platform (86) is disposed on the working platform (100), and a sampling Y-direction platform component (81) is disposed between the platform (100) and the working platform (100). The sampling Y-direction platform component (81) can drive the sampling platform (86) to move along the Y direction. The spotting platform (86) is provided with a spotting deep hole plate placement position (800); The spotting platform (86) is also provided with a plurality of deep well plate placement positions along the X direction. The deep well plate placement positions are all located behind the spotting deep well plate placement position (800). One of the deep well plate placement positions is a nucleic acid extraction placement position (705), which is configured to place the deep well plate containing sample liquid. The other deep well plate placement positions are configured to place consumables required for nucleic acid extraction. The multiple deep well plate placement positions include, sequentially from the spacer along the X direction, the first deep well plate placement position (700), the second deep well plate placement position (701), the third deep well plate placement position (702), the fourth deep well plate placement position (703), the fifth deep well plate placement position (704), and the nucleic acid extraction placement position (705); The clamping and transfer module (6), the spotting module (8), and the nucleic acid extraction module (7) are all located above the spotting platform (86) on the rack (1). The clamping and transfer module (6) and the spotting module (8) can move relative to the rack (1) in the X, Y, and Z directions, and move synchronously in the X direction. The nucleic acid extraction module (7) can move relative to the rack (1) in the X and Z directions. The clamping and transfer module (6) moves the deep well plate containing the sample liquid to the nucleic acid extraction placement position (705); The nucleic acid extraction module (7) is located on the rear side of the plurality of deep well plate placement positions and is used to extract nucleic acid from the sample liquid in the deep well plate at the nucleic acid extraction placement position (705); The clamping and transfer module (6) clamps the deep well plate after nucleic acid extraction to the spotting deep well plate placement position (800), and the spotting module (8) is used to extract a predetermined amount of sample liquid from the deep well plate to the spotting plate to form a nucleic acid test sample; The clamping and transferring module (6) includes: Transfer X-axis moving component, which is mounted on rack (1); A Y-axis moving component (61) is disposed on the X-axis moving component; A Z-axis moving component (62) is disposed on the Y-axis moving component (61), and the Y-axis moving component (61) drives the Z-axis moving component (62) to move along the Y direction; A transfer clamping assembly (63) is disposed on the transfer Z-axis moving assembly (62). The transfer Z-axis moving assembly (62) can drive the transfer clamping assembly (63) to clamp the deep hole plate and drive the deep hole plate to rotate. The transfer clamping assembly (63) includes: A Z-axis guide assembly (631) and a transfer electric gripper (632) connected thereto, the Z-axis guide assembly (631) being connected to the Z-axis moving assembly (62), the Z-axis guide assembly (631) providing guidance for the Z-axis movement of the transfer electric gripper (632); The output end of the transfer electric gripper (632) is provided with a rotating clamping block (633), the rotating clamping block (633) is provided with a clamping groove (6331), and a clamping slider (6332) is provided in the clamping groove (6331) for sliding cooperation. And clamping arms (634), each of the clamping sliders (6332) is connected to a set of clamping arms (634), the two sets of clamping arms (634) can move towards each other or relative to each other in the same direction, and the clamping arms (634) are configured to clamp the two sides of the deep hole plate; The clamping arm (634) includes: The clamping arm body (6341) has one end connected to the clamping slider (6332); A clamping movable block (6342) is connected to the front end of the clamping arm body (6341), and a wear-resistant pad (6345) is provided between the clamping arm body (6341) and the clamping movable block (6342); A connecting rotating member (6343) is arranged axially along the Z direction, and passes through and connects the clamping arm body (6341), the wear-resistant pad (6345), and the clamping movable block (6342). The clamping movable block (6342) can rotate relative to the clamping arm body (6341) along the Z direction by a first preset angle. A second elastic element (6344) is disposed between the clamping movable block (6342) and the connecting rotating element (6343), and the axial direction of the second elastic element (6344) is perpendicular to the side wall of the deep hole plate clamped by the clamping arm body (6341).

2. The automated nucleic acid extraction and sample preparation device according to claim 1, characterized in that, The Y-axis moving component (61) includes: A Y-direction fixed plate (611) is disposed on the X-direction moving assembly; A Y-axis transfer drive assembly (612) is disposed on the Y-axis transfer fixing plate (611); Two sets of second belt pulley assemblies (613), each set of second belt pulley assemblies (613) is connected to the output end of one set of the transfer Y-direction drive assembly (612), and the transfer Z-direction movement assembly (62) is connected to the second belt pulley assembly (613); A Y-axis sliding assembly (614) is disposed on the Y-axis fixed plate (611) and located between the two sets of second belt pulley assemblies (613). The Z-axis moving assembly (62) and the dotting module (8) are both connected to the Y-axis sliding assembly (614) and can slide independently along the Y-axis.

3. The automated nucleic acid extraction and sample preparation device according to claim 2, characterized in that, The Y-axis sliding component (614) includes: A Y-axis sliding member (6142) is disposed on a Y-axis fixed plate (611); Two sets of Y-axis sliding blocks (6141) are slidably disposed on the Y-axis sliding member (6142). The Z-axis moving component (62) is connected to one of the sets of Y-axis sliding blocks (6141), and the Z-axis moving component (62) is also connected to one of the sets of second belt pulley assemblies (613). The sampling module (8) is connected to another set of Y-axis sliding blocks (6141), and the sampling module (8) is also connected to another set of second belt pulley assemblies (613).

4. The automated nucleic acid extraction and sample preparation device according to claim 1, characterized in that, The spotting Y-axis platform component (81) includes: The first linear motor (811) is mounted on the work platform (100); A first Y-axis linear slide (812) for sampling is connected to the output end of the first linear motor (811) for sampling, and the sampling platform (86) is disposed on the first Y-axis linear slide (812); A first sampling guide component (82) is disposed on the working platform (100) and is arranged in parallel with the first sampling Y-axis linear slide (812). The sampling platform (86) is connected to the first sampling guide component (82). The first sampling linear motor (811) can drive the sampling platform (86) located on the first sampling Y-axis linear slide (812) to move along the first sampling guide component (82).

5. The automated nucleic acid extraction and sample preparation device according to claim 2, characterized in that, The nucleic acid extraction module (7) includes: A nucleic acid extraction X-direction driving component (71) is disposed on the working platform (100) and located behind the deep well plate placement position; Nucleic acid extraction rack (72) is disposed on the nucleic acid extraction X-direction driving component (71), which is capable of driving the nucleic acid extraction rack (72) to move along the X direction; A nucleic acid extraction Z-axis drive assembly (73) is disposed on the nucleic acid extraction rack (72); Nucleic acid extraction mounting assembly (74) is connected to the nucleic acid extraction Z-axis drive assembly (73), and the nucleic acid extraction mounting assembly (74) is capable of synchronously moving relative to the nucleic acid extraction frame (72) along the Z-axis. The nucleic acid extraction mounting assembly (74) is capable of gripping or releasing the magnetic rod sleeve. A nucleic acid extraction component (75) is disposed directly above the nucleic acid extraction mounting component (74) and connected to the output end of the nucleic acid extraction mounting component (74). The nucleic acid extraction mounting component (74) can drive the nucleic acid extraction component (75) to move relative to the nucleic acid extraction frame (72) in the Z direction so that the nucleic acid extraction component (75) can be installed or removed from the magnetic rod sleeve.

6. The automated nucleic acid extraction and sample preparation device according to claim 5, characterized in that, The nucleic acid extraction installation component (74) includes: A first mounting bracket (741) is connected to the nucleic acid extraction Z-axis driving assembly (73) and slidably connected to the nucleic acid extraction frame (72). The first mounting bracket (741) is provided with a second opening (70) for the magnetic rod of the nucleic acid extraction assembly (75) to pass through. A first mounting drive assembly (742) is disposed on the first mounting bracket (741) and its output end is connected to the nucleic acid extraction assembly (75). The first mounting drive assembly (742) can drive the nucleic acid extraction assembly (75) to move along the Z direction. A clamping rod assembly (743) passes through the first mounting bracket (741) and is located on both sides of the second opening (70); A rotation drive assembly (744) is disposed on the first mounting bracket (741) and connected to the clamping rod assembly (743). The rotation drive assembly (744) drives the clamping rod assembly (743) to rotate along its own axis to clamp or release the magnetic rod sleeve at the second opening (70).

7. The automated nucleic acid extraction and sample preparation device according to claim 6, characterized in that, The rotation drive assembly (744) includes: A pressing member (441) is disposed on the nucleic acid extraction component (75) and is capable of moving synchronously with the nucleic acid extraction component (75) along the Z direction; A tension spring (7442) has one end connected to the first mounting bracket (741) and the other end connected to the clamping rod assembly (743). The axial direction of the tension spring (7442) forms a second preset angle with the X direction. A pressure contact (7443) is disposed on the clamping rod assembly (743), and when the first mounting drive assembly (742) drives the nucleic acid extraction assembly (75) to move downward along Z, the pressure contact (7443) can slide along the pressure member (441) and press against the locking part of the pressure member (441), and the tension spring (7442) can drive the clamping rod assembly (743) to rotate to release the magnetic rod sleeve.

Images