Dental pulp extraction device for obtaining clean dental pulp stem cells and control method thereof

Abstract

A pulp extraction device for obtaining clean dental pulp stem cells and a control method thereof. The present invention belongs to the technical field of stem cell microtissue extraction, and specifically relates to a pulp extraction device for obtaining clean dental pulp stem cells and a control method thereof. The present invention aims to automatically and accurately cut and extract tooth roots without human and other factors under the premise of ensuring cleanliness. It comprises a pulp extraction tool, a dental pulp stem cell extractor, a dental pulp delivery window, a dental pulp stem cell extraction tube, a dental pulp stem cell extraction tube carrier, a tooth body carrier, a motor, a high-efficiency filter, a tooth body clamp screw, a tooth body universal control assembly, a tooth body clamp, a tooth body clamp, a forward X-ray machine, a lateral X-ray machine, an extraction device main body, a compressor cooling fan and a compressor. Method: the pulp extraction tool is matched with the dental pulp stem cell extractor to quickly extract dental pulp stem cells, and a biological medical standard system is realized. The present invention is used for extracting dental pulp stem cells.

Description

Technical Field

[0001] This invention belongs to the field of stem cell micro-tissue extraction technology, specifically relating to a dental pulp extraction device and its control method for obtaining clean dental pulp stem cells. Background Technology

[0002] Dental pulp tissue, located within the pulp cavity inside the tooth, is the only soft tissue in the tooth structure. Studies have shown that dental pulp cells share remarkably similar immune phenotypes and the ability to form mineralized nodules with bone marrow mesenchymal stem cells. These cells are spindle-shaped, capable of self-renewal and multi-directional differentiation, and possess strong cloning capabilities. Dental pulp stem cells are a type of stem cell with multi-directional differentiation potential. Under in vitro techniques, they can differentiate into various human tissue cells, including fat, bone, cartilage, muscle, vascular endothelium, liver, and nerve cells, and further differentiate into corresponding human tissues. Dental pulp stem cells have been used to treat over 200 diseases, including diabetes, rheumatoid arthritis, cerebral palsy, spinal cord injury, Parkinson's disease, stroke, Alzheimer's disease, periodontitis, and pulp regeneration.

[0003] Extracting dental pulp tissue from the pulp cavity is currently the only method for obtaining dental pulp stem cells. However, due to the diverse structure and limited space of the pulp cavity, the pulp tissue content is very low, making it difficult to extract all the pulp tissue. Existing methods typically involve professionals drilling a hole in the tooth and using a collection device to remove a portion of the pulp, but this carries the risk of exposure and severe damage to stem cells, rendering it impractical. Another extraction method involves breaking the tooth to obtain pulp tissue, relying on non-specialized tools such as pliers and hemostatic forceps to crush the tooth, or wrapping the tooth in gauze and then breaking it with a hammer. However, due to the hardness, irregular shape, and slipperiness of the tooth, this operation is extremely laborious for operators. Furthermore, the crushing process is difficult to control, easily causing fragments to fly and contaminate the dental pulp stem cells. Summary of the Invention

[0004] The present invention aims to solve the technical problems existing in the prior art by providing a dental pulp extraction device and its control method for obtaining clean dental pulp stem cells. Under the premise of ensuring cleanliness, the device automatically and accurately cuts and extracts the tooth root without being affected by human or other factors.

[0005] This invention discloses a dental pulp extraction device for obtaining clean dental pulp stem cells, comprising a dental pulp extraction tool, a dental pulp stem cell extractor, a dental pulp transfer window, a dental pulp stem cell extraction tube, a dental pulp stem cell extraction tube carrier, a tooth body carrier, a motor, a high-efficiency filter, a tooth body clamping screw, a tooth body universal control assembly, a tooth body clamp, a tooth body clamp, a frontal X-ray machine, a side X-ray machine, an extraction device body, a compressor cooling fan, and a compressor; a partition plate is provided at the lower part of the extraction device body, dividing the extraction device body into an operating area and a receiving area; the dental pulp extraction tool is embedded in the partition plate; a top part of the operating area is provided with... The device includes a high-efficiency filter, an upper part of the operating area with a tooth body clamping screw, a tooth body clamp connected to the bottom of the tooth body clamping screw via a tooth body universal control assembly, and a tooth body clamp at the lower part of the tooth body clamp. A pulp transfer window is located on the side of the extraction device body, and both the pulp stem cell extraction tube carrier and the tooth body carrier are located inside the pulp transfer window. The pulp stem cell extraction tube is located inside the pulp stem cell extraction tube carrier. The pulp stem cell extractor, the anteroposterior X-ray machine, and the lateral X-ray machine are all located within the operating area. The compressor cooling fan and the compressor are located within the accommodating area.

[0006] The control method for the dental pulp extraction device for obtaining clean dental pulp stem cells according to the present invention is specifically carried out according to the following steps:

[0007] The tooth body is placed into the tooth body carrier through the pulp transfer window, and the pulp stem cell extraction tube is placed into the pulp stem cell extraction tube carrier. The tooth body clamp is driven by the control program to move and extend on the tooth body clamp screw and the tooth body universal control component. The tooth body clamp picks up the tooth body and acts on the enamel surface. The tooth body clamp returns to the cutting position with the tooth body in its grip. The tooth body is photographed with a front X-ray machine and a side X-ray machine to obtain data on the number, position, front wall thickness and lateral wall thickness of the tooth roots. The tooth roots are cut sequentially according to the data. The tooth body universal control component is controlled to move downward and make a circular motion. The tooth body is cut with an internal tooth saw blade to the designed thickness. The push fork is controlled to move upward to push the lower sleeve, micro-ball bearing and upper sleeve to move upward as a whole, so that the tooth root separation clamp holds the lower end of the tooth body. The micro-ball bearing and upper sleeve rotate under the drive of the motor. The tooth root separation clamp rotates and separates the tooth body.

[0008] The beneficial effects of this invention are:

[0009] 1. This invention is a composite rotary clamping structure that integrates cutting, clamping, fixing, and separation functions. It is suitable for the extraction of single and multiple tooth roots, and can protect the dental pulp from damage during extraction. The internal saw blade can be replaced, effectively reducing maintenance costs.

[0010] 2. This invention can take pictures of the tooth body using a frontal and lateral X-ray machine, calculate the thickness of the tooth root lateral wall using a computer, and extract the pulp stem cell tissue completely by feeding it through the tooth body universal control component and cutting it with a pulp extraction tool.

[0011] 3. Compared with the prior art, the present invention saves manpower, is more precise in operation, can be operated automatically in a sterile environment with constant temperature and humidity, and can realize the automated extraction of large quantities and batches of dental pulp stem cells, thereby improving work efficiency. Attached Figure Description

[0012] Figure 1 A schematic diagram of a dental pulp extraction device for obtaining clean dental pulp stem cells;

[0013] Figure 2 This is a schematic diagram of the dental pulp extraction tool.

[0014] Figure 3 This is a top view of the dental pulp extraction tool.

[0015] Figure 4 This is a schematic diagram showing the chip location of the pulp extraction tool. Detailed Implementation

[0016] Specific Implementation Method 1: A dental pulp extraction device for obtaining clean dental pulp stem cells according to this embodiment includes a dental pulp extraction tool 1, a dental pulp stem cell extractor 2, a dental pulp transfer window 3, a dental pulp stem cell extraction tube 4, a dental pulp stem cell extraction tube carrier 5, a tooth body carrier 7, a motor 10, a high-efficiency filter 11, a tooth body clamping screw 12, a tooth body universal control assembly 13, a tooth body clamp 14, a tooth body clamp 15, a frontal X-ray machine 17, a side X-ray machine 18, an extraction device body 19, a compressor cooling fan 20, and a compressor 21. A partition plate is provided at the lower part of the extraction device body 19, dividing the extraction device body 19 into an operating area and a receiving area. The dental pulp extraction tool 1 is embedded in the partition plate. The operating area... A high-efficiency filter 11 is provided at the top of the device. A tooth body clamping screw 12 is provided at the upper part of the operating area. The bottom of the tooth body clamping screw 12 is connected to a tooth body clamp 14 through a tooth body universal control component 13. A tooth body clamp 15 is provided at the lower part of the tooth body clamp 14. The pulp transfer window 3 is provided on the side of the extraction device body 19. The pulp stem cell extraction tube carrier 5 and the tooth body carrier 7 are both provided inside the pulp transfer window 3. The pulp stem cell extraction tube 4 is provided inside the pulp stem cell extraction tube carrier 5. The pulp stem cell extractor 2, the forward X-ray machine 17 and the side X-ray machine 18 are all provided in the operating area. The compressor cooling fan 20 and the compressor 21 are provided in the accommodating area.

[0017] The extraction device in this embodiment is a sealed unit with a high-efficiency filter at the top and a compressor, cooling fan and exhaust fan at the bottom, which meets the internal Class A purification standard, maintains constant temperature and humidity, and is suitable for pulp extraction. It also uses pulp extraction tooling and a pulp stem cell extractor to quickly extract pulp stem cells, achieving the purpose of extraction according to the biomedical standard system.

[0018] Specific Implementation Method Two: This implementation method differs from Specific Implementation Method One in that the pulp extraction tool 1 is a composite rotary clamping structure; it consists of an internal tooth saw blade 22, fixing screws 23, tool base 24, root cleaving clamp 25, root cleaving clamp arm 26, root cleaving clamp wedge block 27, spring plate 28, fixing pin 29, spring plate fixing nail 30, upper sliding sleeve 31, micro-ball bearing 32, lower sliding sleeve 33, motor drive rod fixing seat 34, and shift fork 9; the bottom of the tool base 24 is provided with a motor drive rod fixing seat 34 for fixing the drive rod of the motor 10; the internal tooth saw blade 22 is fixed to the top of the tool base 24 by fixing screws 23; the root cleaving clamp 25 is fixed... At the front end of the tooth root fracture clamp arm 26, the tooth root fracture clamp arm 26 is fixed to the tooth root fracture clamp wedge block 27; the tooth root fracture clamp 25, the tooth root fracture clamp arm 26, and the tooth root fracture clamp wedge block 27 are an integrated structure and are fixed to the tooling base 24 by a fixing pin 29; the upper sliding sleeve 31, the micro-ball bearing 32, and the lower sliding sleeve 33 form an upper-middle-lower structure, which is ring-shaped and disposed on the outside of the tooling base 24; the shift fork 9 is disposed at the lower part of the lower sliding sleeve 33 to push the upper-middle-lower structure to move vertically upward as a whole; a spring plate 28 is fitted on the inner side of the tooth root fracture clamp wedge block 27, and the spring plate 28 is fixed to the inner side of the tooling base 24 by a spring plate fixing pin 30. Other steps and parameters are the same as in specific embodiment one.

[0019] Specific Implementation Method Three: This implementation method differs from Specific Implementation Method One or Two in that the root detachment clamp 25 has a semi-circular tip structure, is made of tungsten-titanium alloy, has a friction surface on the tip surface, and the distance between the two tips is 2.0 mm. Other steps and parameters are the same as in Specific Implementation Method One or Two.

[0020] Specific Implementation Method Four: This implementation method differs from Specific Implementation Methods One to Three in that the upper sliding sleeve 31, micro-ball bearing 32, lower sliding sleeve 33, and shift fork 9 are relatively linked structures; the lower sliding sleeve 33 does not rotate when in contact with the partition plate, while the micro-ball bearing 32 and upper sliding sleeve 31 rotate under the drive of the motor 10. Other steps and parameters are the same as in Specific Implementation Methods One to Three.

[0021] Specific Implementation Method Five: This implementation method differs from Specific Implementation Methods One to Four in that: after the high-efficiency filter 11 filters the air, it is discharged downward through the cooling fan 20, while the compressor 21 operates to keep the interior of the extraction device body 19 in a constant temperature, humidity, and clean environment. Other steps and parameters are the same as in Specific Implementation Methods One to Four.

[0022] Specific Implementation Method Six: This implementation method differs from Specific Implementation Methods One to Five in that the motor 10 is controlled by a solenoid valve 8, and the solenoid valve 8 is fixed by a plug-in fixing pin 35. Other steps and parameters are the same as in Specific Implementation Methods One to Five.

[0023] Specific Implementation Method Seven: The control method for obtaining the dental pulp extraction device for clean dental pulp stem cells in this implementation method is specifically carried out according to the following steps:

[0024] The tooth body 6 is placed into the tooth body carrier 7 through the pulp transfer window 3, and the pulp stem cell extraction tube 4 is placed into the pulp stem cell extraction tube carrier 5. The tooth body clamp 14 is driven by the control program to move and extend on the tooth body clamp screw 12 and the tooth body universal control component 13. The tooth body clamp 15 picks up the tooth body 6 and acts on the surface of the enamel 16. The tooth body clamp 15 returns the tooth body 6 to the cutting position while holding it. The tooth body 6 is photographed using a forward X-ray machine 17 and a side X-ray machine 18 to obtain the number and location of the tooth roots. The data on the thickness of the front and side walls of the tooth root are used to cut the tooth root sequentially. The tooth body universal control component 13 is controlled to move downward and make a circular motion. The tooth body 6 is cut by the internal saw blade 22 until the designed thickness is reached. The push fork 9 is controlled to move upward and push the lower sleeve 33, micro-ball bearing 32 and upper sleeve 31 to move upward as a whole. The tooth root separation clamp 25 clamps the lower end of the tooth body 6. The micro-ball bearing 32 and upper sleeve 31 rotate under the drive of the motor 10. The tooth root separation clamp 25 rotates and separates the tooth body 6.

[0025] The specific process of this embodiment is as follows: the motor-driven rod fixing seat drives the internal tooth saw to rotate through the rotation of the motor. In conjunction with the tooth body universal control component, the tooth body is rotated. Under the drive control of X-ray imaging and modeling, the dentin is cut. When the preset cutting thickness is reached, the upper sliding sleeve is moved upward by the solenoid valve and the shift fork. The tooth root cleaving forceps are moved inward to make the tooth root cleaving forceps firmly bite the enamel. With the motor rotation, the tooth root is quickly cut off. The pulp stem cell extractor is positioned in the pulp extraction tooling. The pulp tissue is slowly extracted by clockwise rotation and transferred to the pulp stem cell extraction tube. The pulp stem cell extractor puts the pulp tissue into the extraction solution and rotates rapidly in the opposite direction to decompose the pulp tissue into a pulp stem cell suspension.

[0026] Specific Implementation Method Eight: This implementation method differs from Specific Implementation Method Seven in that: after the severance is completed, the dental pulp stem cell extractor 2 is moved to the cutting position, deeply inserted into the severed tooth root, and slowly rotated clockwise to extract the dental pulp tissue and transfer it to the dental pulp stem cell extraction tube 4. The dental pulp tissue is then immersed in the extraction solution and rotated rapidly in the opposite direction to decompose the dental pulp tissue into a dental pulp stem cell suspension. Other steps and parameters are the same as in Specific Implementation Method Seven.

[0027] Specific Implementation Method Nine: This implementation method differs from Specific Implementation Method Seven or Eight in that the designed thickness is 0.1 mm from the inner wall of the pulp cavity to dentin 36. Other steps and parameters are the same as in Specific Implementation Method Seven or Eight.

[0028] The beneficial effects of the present invention are verified using the following embodiments:

[0029] Example 1: A dental pulp extraction device for obtaining clean dental pulp stem cells is performed according to the following steps:

[0030] The control method for obtaining clean dental pulp stem cells using a dental pulp extraction device is specifically carried out according to the following steps:

[0031] The tooth body 6 is placed into the tooth body carrier 7 through the pulp transfer window 3, and the pulp stem cell extraction tube 4 is placed into the pulp stem cell extraction tube carrier 5. The tooth body clamp 14 is driven by a control program to move and extend along the tooth body clamp screw 12 and the tooth body control component 13. The tooth body clamp 15 picks up the tooth body 6 and applies it to the enamel surface 16. The tooth body clamp 15 then returns the tooth body 6 to the cutting position. The tooth body 6 is photographed using a forward X-ray machine 17 and a side X-ray machine 18 to obtain the number and location of the tooth roots. The data on the thickness of the front and side walls of the tooth root are used to cut the tooth root sequentially. The tooth body control component 13 is controlled to move downward and make a circular motion. The tooth body 6 is cut by the internal saw blade 22 until the designed thickness is reached. The push fork 9 is controlled to move upward and push the lower sleeve 33, micro-ball bearing 32 and upper sleeve 31 to move upward as a whole. The tooth root separation clamp 25 clamps the lower end of the tooth body 6. The micro-ball bearing 32 and upper sleeve 31 rotate under the drive of the motor 10. The tooth root separation clamp 25 rotates and separates the tooth root.

[0032] The main body of the dental pulp stem cell extraction device is sealed, with a high-efficiency filter at the top and a compressor, cooling fan, and exhaust fan at the bottom. It meets the internal Class A purification standard, maintains constant temperature and humidity, and is suitable for dental pulp extraction. It is used in conjunction with dental pulp extraction tooling and dental pulp stem cell extractor to quickly extract dental pulp stem cells, achieving the purpose of extraction according to the biomedical standard system.

[0033] Data analysis in this embodiment:

[0034]

[0035]

Claims

1. A dental pulp extraction device for obtaining clean dental pulp stem cells, characterized in that... The dental pulp extraction device for obtaining clean dental pulp stem cells includes a dental pulp extraction tool (1), a dental pulp stem cell extractor (2), a dental pulp transfer window (3), a dental pulp stem cell extraction tube (4), a dental pulp stem cell extraction tube carrier (5), a tooth body carrier (7), a motor (10), a high-efficiency filter (11), a tooth body clamping screw (12), a tooth body universal control assembly (13), a tooth body clamp (14), a tooth body clamp (15), a frontal X-ray machine (17), a side X-ray machine (18), an extraction device body (19), a compressor cooling fan (20), and a compressor (21); the lower part of the extraction device body (19) is provided with a partition plate, which divides the extraction device body (19) into an operating area and a receiving area; the dental pulp extraction tool (1) is embedded in the partition plate; the top of the operating area is provided with a high-efficiency filter. The device includes an effective filter (11), a tooth body clamp screw (12) at the top of the operating area, a tooth body clamp (14) at the bottom of the tooth body clamp screw (12) connected to the tooth body clamp (14) via a tooth body universal control assembly (13), and a tooth body clamp (15) at the bottom of the tooth body clamp (14). The pulp transfer window (3) is located on the side of the extraction device body (19), the pulp stem cell extraction tube carrier (5) and the tooth body carrier (7) are both located inside the pulp transfer window (3), and the pulp stem cell extraction tube (4) is located inside the pulp stem cell extraction tube carrier (5). The pulp stem cell extractor (2), the forward X-ray machine (17) and the side X-ray machine (18) are all located within the operating area. The compressor cooling fan (20) and the compressor (21) are located within the accommodating area. The pulp extraction tool (1) is a composite rotary clamping structure; it consists of an internal tooth saw blade (22), a fixing screw (23), a tool base (24), a root cleaving clamp (25), a root cleaving clamp arm (26), a root cleaving clamp wedge (27), a spring plate (28), a fixing pin (29), a spring plate fixing pin (30), an upper sliding sleeve (31), a micro-ball bearing (32), a lower sliding sleeve (33), a motor drive rod fixing seat (34), and a fork (9); the bottom of the tool base (24) is provided with a motor drive rod fixing seat (34) to fix the drive rod of the motor (10); the internal tooth saw blade (22) is fixed to the top of the tool base (24) by the fixing screw (23); the root cleaving clamp (25) is fixed to the root cleaving clamp arm (28), a tooth tooth saw blade (22), a fixing screw (23), a tooth tooth cleaving clamp arm (26), a tooth tooth cleaving clamp wedge (27), a spring plate (28), a fixing pin (29), a spring plate fixing pin (30), an upper sliding sleeve (31), a micro-ball bearing (32), a lower sliding sleeve (33), a motor drive rod fixing seat (34), and a fork (9); the bottom of the tool base (24) is provided with a motor drive rod fixing seat (34) to fix the motor (10); the internal tooth saw blade (22) is fixed to the top of the tool base (24) by the fixing screw (23); the root cleaving clamp (25) is fixed to the tooth tooth cleaving clamp arm (28), a tooth tooth cleaving clamp arm (29), a tooth tooth cleaving clamp arm (20), a tooth tooth cleaving clamp arm (20), a tooth tooth cleaving clamp arm (26), a tooth tooth cleaving clamp arm (27), a tooth tooth cleaving clamp arm (28), a tooth tooth At the front end of 6), the tooth root cleaver arm (26) is fixed on the tooth root cleaver wedge block (27); the tooth root cleaver (25), the tooth root cleaver arm (26) and the tooth root cleaver wedge block (27) are an integrated structure and are fixed on the tooling base (24) by a fixing pin (29); the upper sliding sleeve (31), the micro-ball bearing (32) and the lower sliding sleeve (33) are an upper-middle-lower structure, and the upper-middle-lower structure is ring-shaped and set on the outside of the tooling base (24); the fork (9) is set on the lower part of the lower sliding sleeve (33) to push the upper-middle-lower structure to move vertically upward as a whole; a spring plate (28) is attached to the inner side of the tooth root cleaver wedge block (27), and the spring plate (28) is fixed to the inner side of the tooling base (24) by a spring plate fixing pin (30); The control method for obtaining clean dental pulp stem cells using a dental pulp extraction device is specifically carried out according to the following steps: The tooth body (6) is placed into the tooth body carrier (7) through the pulp transfer window (3), and the pulp stem cell extraction tube (4) is placed into the pulp stem cell extraction tube carrier (5). The tooth body clamp (14) is driven by the control program to move and extend on the tooth body clamp screw (12) and the tooth body universal control component (13). The tooth body clamp (15) picks up the tooth body (6) and acts on the enamel 16 surface. The tooth body clamp (15) holds the tooth body (6) and returns it to the cutting position. The tooth body (6) is photographed using a forward X-ray machine (17) and a side X-ray machine (18) to obtain the number of tooth roots, The data on the position, frontal wall thickness and lateral wall thickness of the tooth root are used to cut the tooth root in sequence. The tooth body universal control component (13) is controlled to move downward and make a circular motion. The tooth body (6) is cut by the internal tooth saw blade (22) to the designed thickness. The push fork (9) is controlled to move upward to push the lower sleeve (33), micro-ball bearing (32) and upper sleeve (31) to move upward as a whole, so that the tooth root separation clamp (25) clamps the lower end of the tooth body (6). The micro-ball bearing (32) and upper sleeve (31) are rotated under the drive of the motor (10) and separated by the tooth root separation clamp (25).

2. The dental pulp extraction device for obtaining clean dental pulp stem cells according to claim 1, characterized in that... The tooth root detachment clamp (25) has a semi-circular tip structure, is made of tungsten-titanium alloy, has a friction surface on the tip surface, and has a distance of 2.0 mm between the two tips.

3. The dental pulp extraction device for obtaining clean dental pulp stem cells according to claim 1, characterized in that... The upper sliding sleeve (31), micro ball bearing (32), lower sliding sleeve (33) and shift fork (9) are relatively linked structures; the lower sliding sleeve (33) contacts the partition plate without rotating, while the micro ball bearing (32) and upper sliding sleeve (31) rotate under the drive of the motor (10).

4. The dental pulp extraction device for obtaining clean dental pulp stem cells according to claim 1, characterized in that... The high-efficiency filter (11) filters the air and discharges it through the cooling fan (20) while the compressor (21) works to keep the inside of the extraction device body (19) in a constant temperature, humidity and clean environment.

5. The dental pulp extraction device for obtaining clean dental pulp stem cells according to claim 1, characterized in that... The motor (10) is controlled by a solenoid valve (8), which is fixed by a plug-in fixing pin (35).

6. The control method for the dental pulp extraction device for obtaining clean dental pulp stem cells according to claim 1, characterized in that... After the separation is completed, the dental pulp stem cell extractor (2) is moved to the cutting position and inserted into the separated tooth root to extract the dental pulp by slow forward rotation. Under control, it is moved into the dental pulp stem cell extraction tube (4) and inserted into the dental pulp stem cell extraction tube (4) to rotate rapidly in the direction of the tube, so that the dental pulp is separated and mixed under the action of the separation liquid.

7. The control method for the dental pulp extraction device for obtaining clean dental pulp stem cells according to claim 1, characterized in that... The designed thickness is 0.1 mm from the inner wall of the pulp chamber to the dentin (36).

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