A multi-dimensional rock thin section digital automatic acquisition system and acquisition method
By designing a multi-dimensional rock sheet digital automatic acquisition system, and using automated control acquisition modules and image processing modules, the problems of batch automatic acquisition of rock sheets and whole-domain microscopic image acquisition are solved, and efficient image acquisition and stitching are achieved.
Patent Information
- Application Number
- CN202211122441.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-09-15
- Publication Date
- 2025-06-06
- Estimated Expiration
- 2042-09-15
AI Technical Summary
The prior art is difficult to realize batch automatic acquisition of rock flakes and whole-domain microscopic image acquisition, and the image stitching speed is slow and the calculation amount is large.
A multi-dimensional rock sheet digital automatic acquisition system is designed, including computers, databases, image processing modules, microscope modules and acquisition modules. Through the polarizer modules, polarizer modules and sample table modules that are automatically controlled, they realize fully automatic sampling and multi-angle image acquisition of rock sheets. The image processing module automatically stitches, generates super-large images and saves them in the database.
It realizes batch automatic acquisition of rock flakes and whole-domain microscopic image acquisition, improves acquisition efficiency and image stitching speed, and supports fully automatic stitching of 100,000 tensor-level images.
Smart Images

Figure CN115656166B_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the field of rock thin section digitization, and in particular relates to a multi-dimensional rock thin section digitization automatic acquisition system and acquisition method. Background Art
[0002] Rock thin section identification is an important experiment for studying rock composition and structure with the help of polarizing microscopes. However, rock thin sections will age and break if they are stored for too long. Therefore, the electronic preservation of rock thin section microscopic images is of great significance. In the traditional rock thin section identification process, the experimenters select representative areas to take pictures and save them, but they cannot realize the image acquisition of the entire thin section. Especially for rock thin sections with strong heterogeneity, preserving single polarization, orthogonal polarization and fluorescence full thin section images is more conducive to the complete preservation of information and comprehensive understanding of the thin section. Furthermore, some minerals under the microscope have characteristics such as polychromaticity and wavy extinction. It is necessary to collect single polarization and orthogonal polarization images at multiple angles to achieve the effect of maximizing the simulation of microscopic features. At the same time, traditional rock thin section microscopic images are collected slice by slice. When collecting a large number of thin section images, manual sample replacement is required, which is inefficient. It is urgent to realize the automatic batch collection of thin sections.
[0003] CN203444153U discloses a polarized light microscope image automatic acquisition and analysis device, which transforms a traditional polarized light microscope. The transformation module includes a bracket, a substrate, four rubber feet, a stage, a control panel assembly, a movable slide assembly, a camera assembly, and a light source assembly. Through precision mechanical design and grating motor control, the thin slice is kept stationary, and the polarizer is controlled by a computer to rotate to any angle, thereby realizing the relative movement of the thin slice and the polarizer. The invention achieves the effect of simulating thin slice observation under an artificial microscope. However, the collection of mineral characteristics under the microscope is not comprehensive. Only single polarized light and orthogonal polarized light rock image information are collected, and there is no function to collect fluorescent images, and batch collection of thin slices is not realized. Sample collection is single-sheet by sheet, the labor cost of sample change is high, and the collection efficiency is low. At the same time, the prior art does not realize the collection of full-domain microscopic images of thin slice samples.
[0004] CN202794020U discloses a polarized image observation and acquisition device, which transforms a traditional polarized microscope, places the observed object on the stage, turns on the computer, selects the current acquisition mode and interval angle, and can realize automatic acquisition and storage by the system, so as to realize multi-angle single polarization and orthogonal polarization multi-angle acquisition of the measured object. However, it only realizes multi-angle image acquisition of a single image, and fails to realize batch acquisition of thin slices.
[0005] CN112215786A discloses a method for optimizing the splicing of single polarized light and orthogonal polarized light rock thin-section images. The method utilizes the regularity of the movement of the stage when shooting a microscopic thin-section sequence image. During the splicing process, the homography matrix of the image with fewer feature points is calculated separately, and then the fusion position of the image is checked and corrected, thereby improving the problem that some images with fewer feature points cannot be spliced or the complete thin sections that are spliced are misaligned when the images are spliced. At the same time, since the single polarized light image and the orthogonal polarized light sequence image of the same thin section are shot in the same way, the position matrix information of the single polarized light image is used to guide the splicing of the orthogonal polarized light images, thereby greatly reducing the time required for the splicing of the orthogonal polarized light images. However, this method has a large amount of calculation, cannot perform targeted processing based on the characteristics of polarized light microscopic images, and has a slow processing speed.
[0006] In summary, the existing technology has achieved multi-angle image acquisition of a single image, but has failed to achieve batch acquisition of thin slices, and cannot achieve the acquisition of full-area microscopic images of thin slice samples or multi-angle image acquisition. The stitching speed is slow and the calculation amount is large when stitching images. Summary of the invention
[0007] In view of this, the present invention aims to propose a multi-dimensional rock thin section digital automatic acquisition system and acquisition method, which can solve the above problems.
[0008] To achieve the above object, the technical solution of the present invention is implemented as follows: a multi-dimensional rock thin section digital automatic acquisition system, including a computer, a database, an image processing module, a microscope module and an acquisition module;
[0009] The computer is connected to the image processor, the database, the microscope module and the acquisition module;
[0010] The acquisition module and the microscope module work together. The microscope module is provided with an imaging module and a light source module. The acquisition module can convert the light emitted by the light source module into linearly polarized light for polarized light microscopy detection. It can realize the switching between single polarized light detection and orthogonal polarized light detection and realize the detection of orthogonal polarized light at different angles.
[0011] The imaging module takes high-definition polarized light microscopic images of rock slices and sends them to a computer; the image processing module automatically stitches the high-definition polarized light microscopic images in the computer to obtain a stitched super-large image, which is stored in a database.
[0012] Further, the microscope module includes a microscope control module, an imaging module, a fluorescence module, a light source module and an objective lens module; the microscope control module is connected to the imaging module, the fluorescence module, the light source module and the objective lens module, and the imaging module, the fluorescence module, the objective lens module and the light source module are arranged in sequence from top to bottom, and at the same time, the microscope control module controls the operation of the imaging module, the fluorescence module, the light source module and the objective lens module;
[0013] The light source module is provided with an illumination light source, and the light emitted by the illumination light source passes through the sample to be tested and then is transmitted to the objective lens module;
[0014] The light entering from the objective lens module is transmitted to the imaging module through the internal microscopic optical path of the microscope to achieve microscopic imaging;
[0015] The imaging module is provided with a CCD sensor for taking high-definition microscopic images of rock slices; the CCD sensor is provided with an imaging module interface, and the imaging module interface is used to install the imaging module;
[0016] An eyepiece module is also provided between the imaging module and the fluorescence module, and the eyepiece module is provided with an eyepiece interface and a replaceable eyepiece;
[0017] A fluorescent illumination light source is provided in the fluorescent module to emit a fluorescent excitation light source to the sample to be tested, and the fluorescence emitted by the sample is transmitted to the eyepiece module and the imaging module through the objective lens;
[0018] The objective lens module is provided with an objective lens interface and a switchable objective lens to achieve switching of objective lenses with different magnifications.
[0019] Furthermore, the acquisition module includes an acquisition controller, an analyzer module, a sample stage module and a polarizer module; the acquisition controller is connected to the analyzer module, the sample stage module and the polarizer module, and at the same time, the acquisition controller controls the movement and operation of the analyzer module, the sample stage module and the polarizer module;
[0020] The polarizer module is installed between the light source module and the objective lens module. The polarizer module is provided with a polarizer and a stepping motor for driving the polarizer to rotate. The polarizer can convert the light emitted by the light source module into linearly polarized light for polarized light microscopy detection.
[0021] The sample stage module is installed between the polarizer module and the objective lens module, and is used to place multiple rock slices and drive the rock slices to move along the X-axis and the Y-axis to realize fully automatic sampling of rock slices;
[0022] The analyzer module is arranged between the objective lens module and the imaging module. The analyzer module is provided with an analyzer and a stepper motor for driving the analyzer to translate and rotate. The analyzer translates to realize the switching between single polarization detection and orthogonal polarization detection; the analyzer rotates in conjunction with the polarizer to realize the detection of orthogonal polarization at different angles.
[0023] Furthermore, the objective lens is of rotational switching type and is provided with a rotation position sensor. The microscope module is connected to a computer, and the computer can obtain the magnification of the objective lens selected by the microscope in real time; the sample stage module is installed on the lifting slider of the microscope, and the lifting slider can drive the sample stage module to rise and fall to achieve automatic focusing.
[0024] Furthermore, the analyzer module includes a support frame which is assembled and installed by a support member, a first support rod, a machine foot, an adjustment rod, a support seat and a base, and is used to support the entire analyzer module. A shell of the analyzer module is installed on the support frame, and an analyzer connector, a shaft connector, a first motor connecting plate, an analyzer stepping motor, a limit rod, a limit block, an analyzer four-core aviation plug, a second motor connecting plate, a lead screw, a slider and a lead screw seat are arranged in the shell of the analyzer module;
[0025] The limit block is installed on the second motor connecting plate and is located at the end of the limit rod. There are two limit blocks, which are used to limit the moving range of the limit rod to achieve the limit of the horizontal displacement moving range of the first deflection detection stepping motor.
[0026] Two polarization stepper motors and two shaft connectors are provided. The first polarization stepper motor is connected to the polarization connector through the shaft connector. At the same time, the first polarization stepper motor is fixedly connected to the limit rod, and a limit structure is provided at the end of the limit rod, which can cooperate with the limit block to limit the horizontal displacement of the first polarization stepper motor. The second polarization stepper motor is installed on the polarization module housing through the second motor connecting plate. At the same time, the second polarization stepper motor is connected to the lead screw and the lead screw seat in sequence through the shaft connector, and a slider is slidably connected to the lead screw, and the slider is connected to the first polarization stepper motor through the first motor connecting plate. The polarization four-core aviation plug is used to connect the cable to realize the control of the polarization stepper motor.
[0027] Furthermore, the polarizer module includes a containing structure composed of a large base plate and a polarizing module shell, and a fixed connecting member, a second support rod, a vertical plate, a first synchronous pulley, a synchronous belt adjustment plate, a synchronous belt, a polarizing pressure ring, a polarizer, an adjustment inner ring, a polarizing seat, an adjustment outer ring, a synchronous pulley seat, a second synchronous pulley, a polarizing stepping motor, a motor seat and a polarizing four-core aviation plug are arranged in the containing structure;
[0028] The polarizer module is installed on the base of the microscope through an adjustable fixing block, the adjustable fixing block is connected to the fixed connecting piece through a second supporting rod, one end of the second supporting rod is arranged outside the polarizing module housing, and the other end passes through the side wall of the polarizing module housing and is arranged inside the polarizing module housing, one end of the vertical plate is installed on the large bottom plate, and the other end is connected to a motor seat, a polarizing stepping motor is installed on the motor seat, the output end of the polarizing stepping motor drives the first synchronous belt wheel to rotate, and the first synchronous belt wheel and the second synchronous belt wheel are connected through a synchronous belt;
[0029] The synchronous belt adjustment plate is installed on the large bottom plate, the vertical plate is installed on the synchronous belt adjustment plate, and the vertical plate can be adjusted on the synchronous belt adjustment plate. The motor seat is connected to the vertical plate, and the deflection stepping motor is installed on the motor seat. The position of the deflection stepping motor can be adjusted by adjusting the position of the vertical plate on the synchronous belt adjustment plate.
[0030] The polarizing seat is fixedly connected to the large base plate, the adjusting outer ring is connected to the polarizing seat, the adjusting inner ring and the adjusting outer ring are rotatably connected, and the polarizing pressure ring fixes the polarizer on the adjusting inner ring through pressure; the synchronous pulley seat is fixedly connected to the adjusting inner ring, and the second synchronous pulley is fixedly connected to the synchronous pulley seat; the polarizing four-core aviation plug is used to connect cables to realize the control of the polarizing stepping motor.
[0031] Furthermore, the sample stage module includes an X-axis drive mechanism, a Y-axis drive mechanism, a sample stage plate, a sample hole and a sample placement slot; the X-axis drive mechanism and the Y-axis drive mechanism are vertically arranged, and the X-axis drive mechanism and the Y-axis drive mechanism are used to drive the sample stage plate to move along the X-axis or Y-axis; a sample placement slot is arranged on the sample stage plate, and a sample hole is arranged in the middle of the sample placement slot.
[0032] A multi-dimensional rock thin section digital automatic acquisition method, using the multi-dimensional rock thin section digital automatic acquisition system, comprises:
[0033] S1: single polarization image acquisition step;
[0034] S2: Steps for collecting orthogonal polarization images;
[0035] S3: Steps for fluorescence image acquisition;
[0036] S4: Image stitching and calling steps.
[0037] Furthermore, the step S1 includes:
[0038] 1) Place the rock thin section samples to be tested into the sample placement slot on the sample stage module. The number of rock thin section samples is 4-6, placed side by side;
[0039] 2) The computer controls the horizontal movement of the analyzer module so that the analyzer is pulled out; the light emitted by the light source is converted into linearly polarized light after passing through the polarizer, and the linearly polarized light passes through the rock slice sample and enters the microscope objective lens, and then reaches the imaging module of the microscope; the computer controls the horizontal movement of the sample stage module so that the upper left corner of the rock slice sample appears in the field of view of the imaging module;
[0040] The computer automatically focuses based on the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image;
[0041] 3) The computer controls the polarizer in the polarizer module to rotate to an initial angle, and the imaging module simultaneously captures the first single polarized image, and then controls the polarizer to rotate at a fixed angle interval, and continuously captures multiple single polarized images; the captured images are stored in a database;
[0042] 4) The computer controls the movement of the sample stage module according to the magnification, so that the imaging module captures adjacent images of the rock thin section microscopic images, and the overlapping rate of adjacent rock thin section microscopic images is not less than 20%; then the polarizer is controlled to rotate at a fixed angle interval to continuously capture multiple single polarization images; the captured images are stored in a database; the sample stage module continues to move until the first rock thin section completes the capture of all angles in all positions;
[0043] 5) The computer controls the movement of the sample stage module so that the second rock slice moves into the field of view, and the above steps 2) to 4) are repeated, and so on, until all positions and all angles of all rock slice samples are photographed;
[0044] The step S2 comprises:
[0045] 1) Place the rock thin section samples to be tested into the sample placement slot on the sample stage module. The number of rock thin section samples is 4-6, placed side by side;
[0046] 2) The computer controls the horizontal movement of the analyzer module so that the analyzer is inserted; the light emitted by the light source is converted into linearly polarized light after passing through the polarizer, and the linearly polarized light enters the microscope objective after passing through the rock slice sample, and then passes through the analyzer to reach the imaging module of the microscope, ensuring that the polarizer and the analyzer are in an orthogonal relationship; the computer controls the horizontal movement of the sample stage module so that the upper left corner of the rock slice sample appears in the field of view of the imaging module;
[0047] The computer automatically focuses based on the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image;
[0048] 3) The computer controls the analyzer and the polarizer to rotate to an initial angle to ensure that the polarizer and the analyzer are in an orthogonal relationship, and the imaging module simultaneously captures the first single polarized image, and then controls the polarizer to rotate at a fixed angle interval to ensure that the polarizer and the analyzer are in an orthogonal relationship, and continuously captures multiple orthogonal polarized images; the captured images are stored in a database;
[0049] 4) The computer controls the movement of the sample stage module according to the magnification, so that the imaging module captures adjacent images of the rock thin section microscopic images, and the overlapping rate of adjacent rock thin section microscopic images is not less than 20%; then the polarizer is controlled to rotate at a fixed angle interval to ensure that the polarizer and the analyzer are in an orthogonal relationship, and multiple orthogonal polarization images are continuously captured; the captured images are stored in a database; the sample stage module continues to move until the first rock thin section completes the capture of all angles in all positions;
[0050] 5) The computer controls the movement of the sample stage module so that the second rock slice moves into the field of view, and the above steps 2) to 4) are repeated, and so on, until all positions and all angles of all rock slice samples are photographed;
[0051] The step S3 comprises:
[0052] 1) Place the rock thin section samples to be tested into the sample placement slot on the sample stage module. The number of rock thin section samples is 4-6, placed side by side;
[0053] 2) The computer controls the polarizer module to move horizontally, so that the polarizer is pulled out; the light emitted by the fluorescence module is reflected by the spectroscope and reaches the rock thin section sample, stimulating the rock thin section sample to emit fluorescence; the fluorescence emitted by the rock thin section sample enters the microscope objective lens and then reaches the imaging module of the microscope; the computer controls the sample stage module to move horizontally, so that the upper left corner of the rock thin section sample appears in the field of view of the imaging module;
[0054] The computer automatically focuses based on the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image;
[0055] 3) The computer controls the movement of the sample stage module according to the magnification, so that the imaging module captures adjacent images of the rock thin section microscopic images, and the overlapping rate of adjacent rock thin section microscopic images is not less than 20%; a plurality of fluorescent images are continuously captured, and the captured images are stored in a database; the sample stage module continues to move until the first rock thin section completes the capture of all positions;
[0056] 4) The computer controls the movement of the sample stage module so that the second rock slice moves into the field of view, and the above steps 2) to 3) are repeated, and so on, until all positions of all rock slice samples are photographed;
[0057] The step S4 comprises:
[0058] 1) The computer numbers each single polarization microscopic image, orthogonal polarization microscopic image, and fluorescence microscopic image taken;
[0059] 2) The computer performs image preprocessing on adjacent single polarized light microscopic images, and then performs image stitching, and stitches the single polarized light microscopic images corresponding to the same rock thin section sample into a complete high-definition large image; the computer performs image preprocessing on adjacent orthogonal polarized light microscopic images, and then performs image stitching, and stitches the orthogonal polarized light microscopic images corresponding to the same rock thin section sample into a complete high-definition large image; the computer performs image preprocessing on adjacent fluorescence microscopic images, and then performs image stitching, and stitches the fluorescence microscopic images corresponding to the same rock thin section sample into a complete high-definition large image;
[0060] 3) The computer stores the single polarization microscopic image, orthogonal polarization microscopic image and fluorescence microscopic image of the same rock thin section sample as a group in the database; when the computer calls the image from the database, the thumbnail of the high-definition image is displayed on the computer screen, and a selection box is displayed on the thumbnail, and the high-definition image of the position selected by the selection box is displayed in the center of the screen; drag the selection box, and the high-definition image displayed on the screen moves accordingly, realizing convenient observation.
[0061] Furthermore, image stitching includes:
[0062] Image Processing:
[0063] First, randomly select 10-20 pixels in each captured image, and then calculate the median value of the brightness channel of the selected pixels; then obtain the average value of the brightness median value of all images to be stitched;
[0064] Then the brightness of all images is averaged, that is, the brightness of each image is adjusted so that the median brightness value of each image is equal to the average of the median brightness values of all images;
[0065] Image Segmentation:
[0066] Divide each image to be stitched into 10,000 sub-images, and then calculate the texture features of each sub-image;
[0067] Then, the texture features of the edge sub-images of the adjacent images to be stitched are compared, and the sub-images with the same texture features are marked as the same sub-images.
[0068] Image stitching:
[0069] The adjacent images to be stitched are stitched using the marked identical sub-images in the adjacent images to be stitched as the alignment reference; and so on, the stitching of all the images to be stitched is completed to obtain a large field of view stitched image.
[0070] Compared with the prior art, the multi-dimensional rock thin section digital automatic acquisition system and acquisition method of the present invention have the following advantages:
[0071] (1) The present invention combines an automatically controlled polarizer module, an analyzer module and a sample stage module with a microscope, and realizes batch automatic sampling and collection of more than 6 rock thin section samples through computer automatic control, thereby improving the efficiency of rock thin section photo collection;
[0072] (2) The present invention designs novel polarizer modules, analyzer modules and sample stage modules, supports fully automatic multi-angle rotation scanning of thin-film samples in orthogonal polarization and single polarization modes, and the scanning and photographing area moves with the rotation angle; supports rotation correction of the large image of thin-film samples, and reversely rotates the corresponding angle of the large image to ensure the consistency of the shape characteristics of the target particles;
[0073] (3) The image stitching method of the present invention is based on the characteristics of polarized light microscopic images, and performs rapid segmentation and calculation of edge texture features. It has a fast stitching speed and high stitching accuracy, supports fully automatic stitching of hundreds of thousands of images, and adopts a layered and block scheme to achieve image storage of hundreds of G capacity.
[0074] (4) The present invention adopts a three-level access strategy of screen cache, high-speed cache, and pyramid image for ultra-high resolution images, which can realize the rapid display of ultra-large images at different resolutions;
[0075] (5) The system of the present invention supports block output into a universal image format without sacrificing resolution, and also supports thumbnail output of the full image; it supports automatic fusion of overlapping areas of images to solve problems such as uneven lighting and different clarity, and obtain a perfect large-viewing-field stitched image. BRIEF DESCRIPTION OF THE DRAWINGS
[0076] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the accompanying drawings:
[0077] Figure 1 It is a schematic diagram of the overall architecture of the system of the present invention;
[0078] Figure 2 This is a schematic diagram of the polarization detection of the present invention;
[0079] Figure 3 It is a structural schematic diagram of the microscope of the present invention;
[0080] Figure 4 It is the structural principle diagram of the microscope of the present invention;
[0081] Figure 5 is a structural schematic diagram of the polarization analyzer module of the present invention;
[0082] Figure 6 is a schematic structural diagram of the polarizer module of the present invention;
[0083] Figure 7 It is a schematic structural diagram of the sample stage module described in the present invention.
[0084] Description of reference numerals:
[0085] 1. Analyzer module; 2. Polarizer module; 3. Sample stage module; 4. CCD sensor; 5. Eyepiece; 501, eyepiece interface; 6. Objective lens; 601, objective lens interface; 7. Fluorescent illumination source; 8. Sample; 9. Spectroscope; 10. Illumination source;
[0086] 101, polarization analyzer connector; 102, shaft connector; 103, first motor connection board; 104, polarization analyzer stepper motor; 105, limit rod; 106, limit block; 107, polarization analyzer module housing; 108, polarization analyzer four-core aviation plug; 109, second motor connection board; 110, lead screw; 111, support member; 112, first support rod; 113, machine foot; 114, adjustment rod; 115, support seat; 116, base; 117, slider; 118, lead screw seat;
[0087] 201, fixed connector; 202, second support rod; 203, vertical plate; 204, first synchronous pulley; 205, synchronous belt adjustment plate; 206, synchronous belt; 207, large bottom plate; 208, polarizing pressure ring; 209, polarizer; 210, adjustment inner ring; 211, polarizing seat; 212, polarizing module housing; 213, adjustment outer ring; 214, synchronous pulley seat; 215, second synchronous pulley; 216, polarizing stepping motor; 217, motor seat; 218, polarizing four-core aviation plug; 219, adjustment fixing block;
[0088] 301, X-axis driving mechanism; 302, Y-axis driving mechanism; 303, sample stage plate; 304, sample placement slot; 305, sample hole. DETAILED DESCRIPTION
[0089] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments may be combined with each other.
[0090] The present invention will be described in detail below with reference to the accompanying drawings and in conjunction with embodiments.
[0091] Embodiment 1:
[0092] Combination Figure 1 , the present invention is a multi-dimensional rock thin section digital automatic acquisition system, including a computer, a database, an image processing module, a microscope module and an acquisition module;
[0093] The computer is connected to the image processor, the database, the microscope module and the acquisition module;
[0094] The microscope module includes a microscope control module, an imaging module, a fluorescence module, a light source module and an objective lens module; the microscope control module is connected to the imaging module, the fluorescence module, the light source module and the objective lens module; the microscope control module controls the operation of the imaging module, the fluorescence module, the light source module and the objective lens module;
[0095] The acquisition module includes an acquisition controller, an analyzer module 1, a sample stage module 3 and a polarizer module 2; the acquisition controller is connected to the analyzer module 1, the sample stage module 3 and the polarizer module 2, and the acquisition controller controls the movement and operation of the analyzer module 1, the sample stage module 3 and the polarizer module 2;
[0096] like Figure 2 As shown, the acquisition module and the microscope module work together. The microscope module is provided with an imaging module and a light source module. The acquisition module can convert the light emitted by the light source module into linearly polarized light for polarized light microscopy detection, and can realize the switching between single polarization detection and orthogonal polarization detection and realize the detection of orthogonal polarization at different angles.
[0097] Specifically, the polarizer module 2 is provided with a polarizer and a stepper motor for driving the polarizer to rotate. The polarizer converts the light emitted by the light source module into linearly polarized light for polarized light microscopy detection. The sample stage module 3 is used to place a plurality of rock slices and drive the rock slices to move along the X-axis and the Y-axis to realize fully automatic sampling of the rock slices. The sample stage module 3 is installed on the lifting slider 117 of the microscope. The lifting slider 117 can drive the sample stage module 3 to rise and fall to realize automatic focusing. The analyzer module 1 is provided with an analyzer and a stepper motor for driving the analyzer to translate and rotate. The analyzer translates to realize switching between single polarization detection and orthogonal polarization detection. The rotation of the analyzer cooperates with the rotation of the polarizer to jointly realize the detection of orthogonal polarization at different angles.
[0098] The imaging module takes high-definition polarized light microscopic images of rock slices and sends them to a computer; the image processing module automatically stitches the high-definition polarized light microscopic images in the computer to obtain a stitched super-large image, which is stored in a database.
[0099] like Figure 3 and Figure 4 As shown, in the microscope module, the imaging module, the fluorescence module, the objective lens module and the light source module are arranged in sequence from top to bottom; in the description of the present invention, it should be understood that the terms "upper", "lower", "inner", "outer", etc. indicate the orientation or position relationship based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present invention.
[0100] An illumination light source 10 is arranged in the light source module, and the light emitted by the illumination light source 10 passes through the sample to be tested 8 and then is transmitted to the objective lens module;
[0101] The light entering from the objective lens module is transmitted to the imaging module through the internal microscopic optical path of the microscope to achieve microscopic imaging;
[0102] The imaging module is provided with a CCD sensor 4 for taking high-definition microscopic images of rock slices; the CCD sensor 4 is provided with an imaging module interface, and the imaging module interface is used to install the imaging module;
[0103] An eyepiece module is also provided between the imaging module and the fluorescence module, and the eyepiece module is provided with an eyepiece interface 501 and a replaceable eyepiece 5;
[0104] A fluorescent illumination light source 7 is provided in the fluorescent module to emit a fluorescent excitation light source to the sample 8 to be tested. The fluorescence emitted by the sample 8 is transmitted to the eyepiece module and the imaging module through the objective lens.
[0105] The objective lens module is provided with an objective lens interface 601 and a switchable eyepiece 6 to switch eyepieces of different magnifications. The eyepiece 6 is a rotation switch, and a rotation position sensor is provided inside. The microscope module is connected to a computer, and the computer can obtain the magnification of the eyepiece 6 selected by the microscope in real time.
[0106] The polarizer module 2 is installed between the light source module and the objective lens module, and is used to convert the light emitted by the light source module into linear polarized light; the polarizer module 2 is provided with a polarizer and a stepping motor for driving the polarizer to rotate, and the polarizer can convert the light emitted by the light source module into linear polarized light for polarized light microscopy detection;
[0107] The sample stage module 3 is installed between the polarizer module 2 and the objective lens module, and is used to place rock slices for microscopic detection; specifically, it can be used to place multiple rock slices and drive the rock slices to move along the X-axis and the Y-axis to achieve fully automatic sampling of rock slices;
[0108] The analyzer module 1 is arranged between the objective lens module and the imaging module, and is used to cooperate with the polarizer module 2 to realize orthogonal polarization detection. Specifically, the analyzer module 1 is provided with an analyzer and a stepper motor for driving the analyzer to translate and rotate. The analyzer translates to realize the switching between single polarization detection and orthogonal polarization detection; the analyzer rotates in conjunction with the polarizer to realize the detection of orthogonal polarization at different angles.
[0109] like Figure 5As shown, the analyzer module 1 includes a support frame which is assembled and installed by a support member 111, a first support rod 112, a machine foot 113, an adjustment rod 114, a support seat 115 and a base 116, and is used to support the entire analyzer module 1. A analyzer module housing 107 is installed on the support frame, and an analyzer connector 101, a shaft connector 102, a first motor connecting plate 103, an analyzer stepping motor 104, a limit rod 105, a limit block 106, an analyzer four-core aviation plug 108, a second motor connecting plate 109, a screw rod 110, a slider 117 and a screw rod seat 118 are arranged in the analyzer module housing 107;
[0110] The limit block 106 is fixed on the second motor connecting plate 109, and the limit block 106 is located at the end of the limit rod 105. There are two limit blocks 106, which are respectively arranged on both sides of the end of the limit rod 105, and are used to limit the movement range of the limit rod 105, so as to limit the horizontal displacement movement range of the first deflection stepping motor 104;
[0111] There are two polarization stepper motors 104 and two shaft connectors 102. The first polarization stepper motor 104 is connected to the polarization connector 101 through the shaft connector 102. At the same time, the first polarization stepper motor 104 is fixedly connected to the limit rod 105, and a limit structure is set at the end of the limit rod 105, which can cooperate with the limit block 106 to limit the horizontal displacement of the first polarization stepper motor 104. The second polarization stepper motor 104 is installed on the polarization module housing 107 through the second motor connecting plate 109. At the same time, the second polarization stepper motor 104 is connected to the lead screw 110 and the lead screw seat 118 in sequence through the shaft connector 102, and the lead screw 110 is slidably connected with a slider 117, and the slider 117 is connected to the first polarization stepper motor 104 through the first motor connecting plate 103. Along with the horizontal movement of the slider 117 on the lead screw 110, the first motor connecting plate 103 will drive the first polarization stepper motor 104 to achieve horizontal movement, thereby realizing the insertion and removal of the polarizer. The polarization four-core aviation plug 108 is used to connect the cable to control the polarization stepping motor 104. In the description of the present invention, it should be understood that the terms "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Therefore, the features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features.
[0112] like Figure 6As shown, the polarizer module 2 includes a containing structure composed of a large base plate 207 and a polarizing module housing 212, and a fixed connecting member 201, a second support rod 202, a vertical plate 203, a first synchronous pulley 204, a synchronous belt adjustment plate 205, a synchronous belt 206, a polarizing pressure ring 208, a polarizer 209, an adjustment inner ring 210, a polarizing seat 211, an adjustment outer ring 213, a synchronous pulley seat 214, a second synchronous pulley 215, a polarizing stepping motor 216, a motor seat 217 and a polarizing four-core aviation plug 218 are arranged in the containing structure;
[0113] The polarizer module 2 is installed on the base 116 of the microscope through an adjustment fixing block 219, and the adjustment fixing block 219 is connected to the fixed connector 201 through a second support rod 202, one end of the second support rod 202 is arranged outside the polarizing module housing 212, and the other end passes through the side wall of the polarizing module housing 212 and is arranged inside the polarizing module housing 212, one end of the vertical plate 203 is installed on the large bottom plate 207, and the other end is connected to a motor seat 217, and a polarizing stepping motor 216 is installed on the motor seat 217, and the output end of the polarizing stepping motor 216 drives the first synchronous belt wheel 204 to rotate, and the first synchronous belt wheel 204 and the second synchronous belt wheel 215 are connected through a synchronous belt 206, so that the polarizing stepping motor 216 can drive the second synchronous belt wheel 215 to rotate;
[0114] The synchronous belt adjustment plate 205 is used to adjust the contact between the first synchronous belt pulley 204 and the synchronous belt 206. Specifically, the synchronous belt adjustment plate 205 is installed on the large base plate 207, the vertical plate 203 is installed on the synchronous belt adjustment plate 205, and the vertical plate 203 can be adjusted in position on the synchronous belt adjustment plate 205. The motor seat 217 is connected to the vertical plate 203, and the deflection stepper motor 216 is installed on the motor seat 217, so that the position of the deflection stepper motor 216 can be adjusted by adjusting the position of the vertical plate 203 on the synchronous belt adjustment plate 205, and the tightness of the synchronous belt 206 is further adjusted to adjust the contact between the first synchronous belt pulley 204 and the synchronous belt 206.
[0115] The polarizing pressure ring 208, the adjusting inner ring 210, the polarizing seat 211, the adjusting outer ring 213 and the synchronous pulley seat 214 are used to realize the connection and installation between the second synchronous pulley 215 and the polarizer 209. Specifically, the polarizing seat 211 is fixedly connected to the large bottom plate 207, the adjusting outer ring 213 is connected to the polarizing seat 211, the adjusting inner ring 210 and the adjusting outer ring 213 are rotatably connected, and the polarizer 209 is fixed to the adjusting inner ring 210 by the polarizing pressure ring 208 through pressure; the synchronous pulley seat 214 is fixedly connected to the adjusting inner ring 210, and the second synchronous pulley 215 is fixedly connected to the synchronous pulley seat 214; thus, when the synchronous belt 206 rotates, it can drive the second synchronous pulley 215 and the synchronous pulley seat 214 to rotate synchronously; further drive the polarizer 209 installed on the adjusting inner ring 210 to rotate. The polarizing four-core aviation plug 218 is used to connect the cable to realize the control of the polarizing stepping motor 216.
[0116] like Figure 7 As shown, the sample stage module 3 includes an X-axis driving mechanism 301, a Y-axis driving mechanism 302, a sample stage plate 303, a sample hole 305 and a sample placement slot 304; the X-axis driving mechanism 301 and the Y-axis driving mechanism 302 are vertically arranged, and the X-axis driving mechanism 301 and the Y-axis driving mechanism 302 are used to drive the sample stage plate 303 to move along the X-axis or the Y-axis; a sample placement slot 304 is arranged on the sample stage plate 303, and a sample hole 305 is arranged in the middle of the sample placement slot 304.
[0117] Embodiment 2:
[0118] A multi-dimensional rock thin section digital automatic acquisition method, using the multi-dimensional rock thin section digital automatic acquisition system, comprises:
[0119] S1: single polarization image acquisition step;
[0120] 1-1) placing the rock slice samples 8 to be tested into the sample placement slot 304 on the sample stage module 3, with 4 to 6 rock slice samples 8 placed side by side;
[0121] 1-2) The computer controls the polarizer module 1 to move horizontally, so that the polarizer is pulled out; the light emitted by the light source is converted into linearly polarized light after passing through the polarizer, and the linearly polarized light passes through the rock thin section sample 8 and enters the microscope eyepiece 6, and then reaches the imaging module of the microscope; the computer controls the sample stage module 3 to move horizontally, so that the upper left corner of the rock thin section sample 8 appears in the field of view of the imaging module;
[0122] The computer automatically focuses based on the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image;
[0123] 1-3) The computer controls the polarizer in the polarizer module 2 to rotate to an initial angle, and the imaging module simultaneously captures the first single polarized image, and then controls the polarizer to rotate at a fixed angle interval, and continuously captures multiple single polarized images; the captured images are stored in a database;
[0124] 1-4) The computer controls the movement of the sample stage module 3 according to the magnification, so that the imaging module captures adjacent images of the rock thin section microscopic images, and the overlapping rate of adjacent rock thin section microscopic images is not less than 20%; then the polarizer is controlled to rotate at a fixed angle interval to continuously capture multiple single polarized images; the captured images are stored in the database; the sample stage module 3 continues to move until the first rock thin section completes the capture of all angles in all positions;
[0125] 1-5) The computer controls the movement of the sample stage module 3 so that the second rock slice moves into the field of view, and the above steps 1-2) to 1-4) are repeated, and so on, until all positions and all angles of all rock slice samples 8 are photographed;
[0126] S2: Steps for collecting orthogonal polarization images;
[0127] 2-1) placing the rock slice samples 8 to be tested into the sample placement slot 304 on the sample stage module 3, with 4 to 6 rock slice samples 8 placed side by side;
[0128] 2-2) The computer controls the analyzer module 1 to move horizontally so that the analyzer is inserted; the light emitted by the light source is converted into linearly polarized light after passing through the polarizer, and the linearly polarized light passes through the rock thin section sample 8 and enters the microscope eyepiece 6, and then passes through the analyzer to reach the imaging module of the microscope, ensuring that the polarizer and the analyzer are in an orthogonal relationship; the computer controls the sample stage module 3 to move horizontally so that the upper left corner of the rock thin section sample 8 appears in the field of view of the imaging module;
[0129] The computer automatically focuses based on the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image;
[0130] 2-3) The computer controls the analyzer and the polarizer to rotate to an initial angle to ensure that the polarizer and the analyzer are in an orthogonal relationship, and the imaging module simultaneously captures the first single polarized image, and then controls the polarizer to rotate at a fixed angle interval to ensure that the polarizer and the analyzer are in an orthogonal relationship, and continuously captures multiple orthogonal polarized images; the captured images are stored in a database;
[0131] 2-4) The computer controls the movement of the sample stage module 3 according to the magnification, so that the imaging module captures adjacent images of the rock thin section microscopic images, and the overlapping rate of adjacent rock thin section microscopic images is not less than 20%; then the polarizer is controlled to rotate at a fixed angle interval to ensure that the polarizer and the analyzer are in an orthogonal relationship, and multiple orthogonal polarization images are continuously captured; the captured images are stored in the database; the sample stage module 3 continues to move until the first rock thin section completes the shooting of all angles in all positions;
[0132] 2-5) The computer controls the movement of the sample stage module 3 so that the second rock slice moves into the field of view, and the above steps 2-2) to 2-4) are repeated, and so on, until all positions and all angles of all rock slice samples 8 are photographed;
[0133] S3: Steps for fluorescence image acquisition;
[0134] 3-1) placing the rock slice samples 8 to be tested into the sample placement slot 304 on the sample stage module 3, with 4 to 6 rock slice samples 8 placed side by side;
[0135] 3-2) The computer controls the polarizer module 1 to move horizontally, so that the polarizer is pulled out; the light emitted by the fluorescence module is reflected by the spectroscope 9 and reaches the rock slice sample 8, stimulating the rock slice sample 8 to emit fluorescence; the fluorescence emitted by the rock slice sample 8 enters the microscope eyepiece 6 and then reaches the imaging module of the microscope; the computer controls the sample stage module 3 to move horizontally, so that the upper left corner of the rock slice sample 8 appears in the field of view of the imaging module;
[0136] The computer automatically focuses based on the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image;
[0137] 3-3) The computer controls the movement of the sample stage module 3 according to the magnification, so that the imaging module captures adjacent images of the rock thin section microscopic images, and the overlapping rate of adjacent rock thin section microscopic images is not less than 20%; a plurality of fluorescent images are continuously captured, and the captured images are stored in a database; the sample stage module 3 continues to move until the first rock thin section completes the capture of all positions;
[0138] 3-4) The computer controls the movement of the sample stage module 3 so that the second rock slice moves into the field of view, and the above steps 3-2) to 3-3) are repeated, and so on, until all positions of all rock slice samples 8 are photographed;
[0139] S4: Image stitching and calling steps.
[0140] 4-1) The computer numbers each single polarized light microscopic image, orthogonal polarized light microscopic image, and fluorescence microscopic image taken;
[0141] 4-2) The computer performs image preprocessing on adjacent single polarized light microscopic images, and then performs image stitching, and stitches the single polarized light microscopic images corresponding to the same rock thin section sample 8 into a complete high-definition large image; the computer performs image preprocessing on adjacent orthogonal polarized light microscopic images, and then performs image stitching, and stitches the orthogonal polarized light microscopic images corresponding to the same rock thin section sample 8 into a complete high-definition large image; the computer performs image preprocessing on adjacent fluorescence microscopic images, and then performs image stitching, and stitches the fluorescence microscopic images corresponding to the same rock thin section sample 8 into a complete high-definition large image;
[0142] 4-3) The computer stores the single polarization microscopic image, orthogonal polarization microscopic image and fluorescence microscopic image of the same rock thin section sample 8 as a group in the database; when the computer calls the image in the database, the thumbnail of the high-definition image is displayed on the computer screen, and a selection box is displayed on the thumbnail, and the high-definition image of the position selected by the selection box is displayed in the center of the screen; drag the selection box, and the high-definition image displayed on the screen moves accordingly, so as to achieve convenient observation.
[0143] The image stitching method is:
[0144] Image Processing:
[0145] First, randomly select 10-20 pixels in each captured image, and then calculate the median value of the brightness channel of the selected pixels; then obtain the average value of the brightness median value of all images to be stitched;
[0146] Then the brightness of all images is averaged, that is, the brightness of each image is adjusted so that the median brightness value of each image is equal to the average of the median brightness values of all images;
[0147] Image Segmentation:
[0148] Divide each image to be stitched into 10,000 sub-images, and then calculate the texture features of each sub-image;
[0149] Then, the texture features of the edge sub-images of the adjacent images to be stitched are compared, and the sub-images with the same texture features are marked as the same sub-images.
[0150] Image stitching:
[0151] The adjacent images to be stitched are stitched using the marked identical sub-images in the adjacent images to be stitched as the alignment reference; and so on, the stitching of all the images to be stitched is completed to obtain a large field of view stitched image.
[0152] Embodiment 3:
[0153] The microscope system adopts Leica DM2500P / DM2700P series microscope, or DM4500P microscope. The microscope has a 6X M25 objective lens rotating disk, which is adjustable and memorizable. After connecting to the computer, the computer automatically recognizes the 6x magnification of the eyepiece; the light source is a 12V-100W halogen lamp, or an LED light source, with automatic light intensity tracking and constant color temperature functions, ensuring that the color temperature and brightness of the images taken in any lighting environment are constant, reducing the amount of subsequent image preprocessing calculations.
[0154] The self-designed acquisition controller, the analyzer module 1, the sample stage module 3 and the polarizer module 2 are installed in the microscope system, and the self-designed supporting control software is installed in the computer; the single polarized light image is acquired by using the method described in Example 2, and 6 rock slices are selected to automatically acquire images;
[0155] The single polarization detection and cross polarization detection are automatically controlled by a computer-controlled microscope, polarizer module 2, analyzer module 1 and sample stage module 3, without manual supervision throughout the process; the computer controls the polarizer module 2 to automatically rotate at a certain angle interval, and controls the analyzer module 1 to automatically insert, remove or rotate according to the detection requirements. The computer controls the microscope to automatically identify the magnification, the sample stage position coordinates, the brightness of the captured image, and automatically collect and store images; multiple rock slices are placed on the sample stage module 3, and continuous collection is automatically performed without any manual intervention.
[0156] The device of the present invention can perform single polarization detection or orthogonal polarization detection alone, and can also perform single polarization and orthogonal polarization detection continuously.
[0157] When single polarization and orthogonal polarization are detected continuously, an image of single polarization detection is collected first, and then the working parameters of other devices are kept unchanged, the polarizer module 1 is directly controlled to be automatically inserted, and then the orthogonal polarization image is directly collected; in this way, single polarization detection and orthogonal polarization detection can be collected continuously at the same time, saving the time of focusing and position adjustment.
[0158] As mentioned above, the sample stage module 3 of the present invention can be automatically raised and lowered under the control of a computer, and the Z-axis position can be automatically adjusted by utilizing the lifting freedom of the microscope module. In conjunction with the automatic focusing function of the microscope, automatic focusing can be achieved during detection without manual adjustment.
[0159] Furthermore, the microscope of the present invention is also provided with an insertion port for a gypsum test plate and a mica test plate, and a gypsum test plate or a mica test plate can be inserted as needed to improve the display effect of polarized light microscopic detection; the gypsum test plate is made of a crystal wafer, and the optical path difference is 530nm-550nm. The optical path difference of the mica test plate is about one-fourth of the wavelength of yellow light, that is, about 147nm, and it presents a primary gray-white interference color between orthogonal polarizers. After adding the mica test plate to the thin slice, the interference color order of the thin slice is raised or lowered by about one color order according to the color spectrum table order. This test plate is more suitable for mineral slices with higher interference colors.
[0160] Each rock thin section collects 128×128=16384 high-definition microscopic images. The collection time of each image is 0.2s, and the collection interval is 0.2s. It takes less than 2 hours to complete the collection of each rock thin section. A total of 98,304 images are collected from 6 rock thin sections, which takes about 12 hours. The system supports automatic stitching of up to 100,000 images. Each collected rock thin section microscopic image is automatically stitched to obtain full-sample, ultra-large-format, and ultra-high-resolution rock thin section images. Stitching is performed while shooting, and stitching can be completed immediately after shooting is completed.
[0161] After stitching is completed, the large image will be saved in the computer database and displayed as a thumbnail when needed to increase browsing speed; after selecting a detailed view position, a high-definition large image of the corresponding position will be displayed on the monitor; position marking and drag browsing are supported during the display process.
[0162] Embodiment 4:
[0163] The microscope system adopts Leica DM2500P / DM2700P series microscope, and the self-designed acquisition controller, analyzer module 1, sample stage module 3 and polarizer module 2 are installed in the microscope system, and the self-designed supporting control software is installed in the computer; the orthogonal polarization image acquisition is performed using the method described in the embodiment, and 6 rock slices are selected to automatically acquire images;
[0164] Each rock thin section collects 50×50=2500 high-definition microscopic images. The collection time for each image is 0.5s, and the collection interval is 0.5s. The collection of each rock thin section takes less than 1 hour. A total of 15,000 images are collected from 6 rock thin sections, which takes about 6 hours. The system supports automatic stitching of up to 100,000 images. Each collected rock thin section microscopic image is automatically stitched together to obtain full-sample, ultra-large-format, and ultra-high-resolution rock thin section images. Stitching is performed while shooting, and stitching can be completed immediately after shooting is completed.
[0165] After stitching is completed, the large image will be saved in the computer database and displayed as a thumbnail when needed to increase browsing speed; after selecting a detailed view position, a high-definition large image of the corresponding position will be displayed on the monitor; position marking and drag browsing are supported during the display process.
[0166] It is worth pointing out that the acquisition time in the embodiment is directly related to the number of microscopic images to be stitched. At the same time, the acquisition time and acquisition interval of each image can be adjusted according to the imaging effect of the equipment. The fastest acquisition interval can be 0.1s and the acquisition time can be 0.1s. Since it supports automatic stitching of 100,000 images, theoretically a single rock slice can take up to 100,000 images. In actual use, the required number of shots can be selected according to needs, and generally more than 400 shots are required.
[0167] After the stitched images are saved, digital storage of rock thin sections can be achieved, avoiding the deterioration of samples due to environmental factors during long-term storage; at the same time, the use of digital technology for preservation can greatly improve the convenience of data transmission and sharing, providing assistance for scientific research.
[0168] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims
1. A multi-dimensional rock thin section digital automatic acquisition system, Features: It includes a computer, a database, an image processing module, a microscope module and an acquisition module; The computer is connected to the image processor, the database, the microscope module and the acquisition module; The acquisition module and the microscope module work together. The microscope module is provided with an imaging module and a light source module. The acquisition module can convert the light emitted by the light source module into linearly polarized light for polarized light microscopy detection. It can realize the switching between single polarized light detection and orthogonal polarized light detection and realize the detection of orthogonal polarized light at different angles. The imaging module takes high-definition polarized light microscopic images of rock slices and sends them to a computer; the image processing module automatically stitches the high-definition polarized light microscopic images in the computer to obtain a stitched super-large image, which is stored in a database; The acquisition module comprises an acquisition controller, an analyzer module (1), a sample stage module (3) and a polarizer module (2); the acquisition controller is connected to the analyzer module (1), the sample stage module (3) and the polarizer module (2), and the acquisition controller controls the movement and operation of the analyzer module (1), the sample stage module (3) and the polarizer module (2); The polarizer module (2) is installed between the light source module and the objective lens module. The polarizer module (2) is provided with a polarizer and a stepping motor for driving the polarizer to rotate. The polarizer can convert the light emitted by the light source module into linearly polarized light for polarized light microscopy detection. The sample stage module (3) is installed between the polarizer module (2) and the objective lens module, and is used to place a plurality of rock slices and drive the rock slices to move along the X-axis and the Y-axis, so as to realize fully automatic sampling of the rock slices; The analyzer module (1) is arranged between the objective lens module and the imaging module. The analyzer module (1) is provided with an analyzer and a stepping motor for driving the analyzer to translate and rotate. The analyzer translates to realize the switching between single polarization detection and orthogonal polarization detection. The analyzer rotates in coordination with the polarizer to realize the detection of orthogonal polarization at different angles. The analyzer module (1) comprises a support frame which is assembled and installed by a support member (111), a first support rod (112), a machine foot (113), an adjustment rod (114), a support seat (115) and a base (116), and is used to support the entire analyzer module (1); a analyzer module housing (107) is installed on the support frame; and an analyzer connector (101), a shaft connector (102), a first motor connecting plate (103), an analyzer stepping motor (104), a limit rod (105), a limit block (106), an analyzer four-core aviation plug (108), a second motor connecting plate (109), a screw rod (110), a slider (117) and a screw rod seat (118) are arranged in the analyzer module housing (107); The limit block (106) is mounted on the second motor connecting plate (109), and the limit block (106) is located at the end of the limit rod (105). There are two limit blocks (106) for limiting the moving range of the limit rod (105) to achieve the limitation of the horizontal displacement moving range of the first deflection detection stepping motor (104); Two polarization stepper motors (104) and two shaft connectors (102) are provided. The first polarization stepper motor (104) is connected to the polarization connector (101) through the shaft connector (102). At the same time, the first polarization stepper motor (104) is fixedly connected to the limit rod (105), and the end of the limit rod (105) is provided with a limit structure, which can cooperate with the limit block (106) to limit the horizontal displacement of the first polarization stepper motor (104). The second polarization stepper motor (104) is connected to the second motor connector (106). The connecting plate (109) is installed on the polarization module housing (107). At the same time, the second polarization stepping motor (104) is connected to the lead screw (110) and the lead screw seat (118) in sequence through the shaft connector (102), and the lead screw (110) is slidably connected to a slider (117), and the slider (117) is connected to the first polarization stepping motor (104) through the first motor connecting plate (103). The polarization four-core aviation plug (108) is used to connect the cable to realize the control of the polarization stepping motor (104).
2. The multi-dimensional rock thin section digital automatic acquisition system according to claim 1, Features: The microscope module includes a microscope control module, an imaging module, a fluorescence module, a light source module and an objective lens module; the microscope control module is connected to the imaging module, the fluorescence module, the light source module and the objective lens module, and the imaging module, the fluorescence module, the objective lens module and the light source module are arranged in sequence from top to bottom, and at the same time, the microscope control module controls the work of the imaging module, the fluorescence module, the light source module and the objective lens module; An illumination light source (10) is arranged in the light source module, and light emitted by the illumination light source (10) passes through the sample to be tested (8) and then is transmitted to the objective lens module; The light entering from the objective lens module is transmitted to the imaging module through the internal microscopic optical path of the microscope to achieve microscopic imaging; A CCD sensor (4) is provided in the imaging module for capturing a high-definition microscopic image of a rock slice; the CCD sensor (4) is provided with an imaging module interface, and the imaging module interface is used to install the imaging module; An eyepiece module is also provided between the imaging module and the fluorescence module, and the eyepiece module is provided with an eyepiece interface (501) and a replaceable eyepiece (5); A fluorescent illumination light source (7) is arranged in the fluorescent module to emit a fluorescent excitation light source to the sample (8) to be tested, and the fluorescence emitted by the sample (8) is transmitted to the eyepiece module and the imaging module through the objective lens; The objective lens module is provided with an objective lens interface (601) and a switchable eyepiece (6) to achieve switching of eyepieces (6) with different magnifications.
3. The multi-dimensional rock thin section digital automatic acquisition system according to claim 2, Features: The eyepiece (6) is of a rotation switching type and is provided with a rotation position sensor therein; the microscope module is connected to a computer, and the computer can obtain the magnification of the eyepiece (6) selected by the microscope in real time; the sample stage module (3) is installed on a lifting slider (117) of the microscope, and the lifting slider (117) can drive the sample stage module (3) to rise and fall to achieve automatic focusing.
4. The multi-dimensional rock thin section digital automatic acquisition system according to claim 1, Features: The polarizer module (2) comprises a containing structure composed of a large base plate (207) and a polarizing module shell (212), wherein a fixed connecting member (201), a second support rod (202), a vertical plate (203), a first synchronous belt pulley (204), a synchronous belt adjustment plate (205), a synchronous belt (206), a polarizing pressure ring (208), a polarizer (209), an adjustment inner ring (210), a polarizing seat (211), an adjustment outer ring (213), a synchronous belt pulley seat (214), a second synchronous belt pulley (215), a polarizing stepping motor (216), a motor seat (217) and a polarizing four-core aviation plug (218) are arranged in the containing structure; The polarizer module (2) is installed on the base (116) of the microscope through an adjustable fixing block (219); the adjustable fixing block (219) is connected to the fixed connection member (201) through a second support rod (202); one end of the second support rod (202) is arranged outside the polarizing module housing (212); the other end passes through the side wall of the polarizing module housing (212) and is arranged inside the polarizing module housing (212); one end of the vertical plate (203) is installed on the large bottom plate (207); the other end is connected to a motor seat (217); a polarizing stepping motor (216) is installed on the motor seat (217); the output end of the polarizing stepping motor (216) drives the first synchronous belt wheel (204) to rotate; the first synchronous belt wheel (204) and the second synchronous belt wheel (215) are connected through a synchronous belt (206); The synchronous belt adjustment plate (205) is mounted on the large bottom plate (207), the vertical plate (203) is mounted on the synchronous belt adjustment plate (205), and the vertical plate (203) can be adjusted in position on the synchronous belt adjustment plate (205); the motor seat (217) is connected to the vertical plate (203), and the deflection stepping motor (216) is mounted on the motor seat (217); and the position of the deflection stepping motor (216) can be adjusted by adjusting the position of the vertical plate (203) on the synchronous belt adjustment plate (205); The polarizing seat (211) is fixedly connected to the large base plate (207); the adjusting outer ring (213) is connected to the polarizing seat (211); the adjusting inner ring (210) and the adjusting outer ring (213) are rotatably connected; the polarizing pressure ring (208) fixes the polarizer (209) on the adjusting inner ring (210) by pressure; the synchronous pulley seat (214) is fixedly connected to the adjusting inner ring (210); the second synchronous pulley (215) is fixedly connected to the synchronous pulley seat (214); and the polarizing four-core aviation plug (218) is used to connect cables to realize the control of the polarizing stepping motor (216).
5. The multi-dimensional rock thin section digital automatic acquisition system according to claim 1, Features: The sample stage module (3) comprises an X-axis driving mechanism (301), a Y-axis driving mechanism (302), a sample stage plate (303), a sample hole (305) and a sample placement groove (304); the X-axis driving mechanism (301) and the Y-axis driving mechanism (302) are arranged vertically, and the X-axis driving mechanism (301) and the Y-axis driving mechanism (302) are used to drive the sample stage plate (303) to move along the X-axis or the Y-axis; the sample stage plate (303) is provided with a sample placement groove (304), and the sample hole (305) is provided in the middle of the sample placement groove (304).
6. A multi-dimensional rock thin section digital automatic acquisition method, using the multi-dimensional rock thin section digital automatic acquisition system according to any one of claims 1 to 5, It is characterized in that include: S1: single polarization image acquisition step; S2: Steps for collecting orthogonal polarization images; S3: Steps for fluorescence image acquisition; S4: Image stitching and calling steps.
7. The multi-dimensional rock thin section digital automatic acquisition method according to claim 6, Features: The step S1 comprises: 1) placing the rock thin section samples (8) to be tested into the sample placement slot (304) on the sample stage module (3), wherein the number of the rock thin section samples (8) is 4 to 6, and the samples are placed side by side; 2) The computer controls the polarizer module (1) to move horizontally, so that the polarizer is pulled out; the light emitted by the light source is converted into linearly polarized light after passing through the polarizer, and the linearly polarized light passes through the rock thin section sample (8) and enters the microscope eyepiece (6), and then reaches the imaging module of the microscope; the computer controls the sample stage module (3) to move horizontally, so that the upper left corner of the rock thin section sample (8) appears in the field of view of the imaging module; The computer automatically focuses based on the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image; 3) The computer controls the polarizer in the polarizer module (2) to rotate to an initial angle, and the imaging module simultaneously captures the first single polarized image, and then controls the polarizer to rotate at a fixed angle interval, and continuously captures multiple single polarized images; the captured images are stored in a database; 4) The computer controls the movement of the sample stage module (3) according to the magnification, so that the imaging module captures adjacent images of the rock thin section microscopic images, and the overlapping rate of adjacent rock thin section microscopic images is not less than 20%; then the polarizer is controlled to rotate at a fixed angle interval to continuously capture multiple single polarization images; the captured images are stored in a database; the sample stage module (3) continues to move until the first rock thin section completes the capture of all angles in all positions; 5) The computer controls the movement of the sample stage module (3) so that the second rock slice moves into the field of view, and the above steps 2) to 4) are repeated, and so on, until all positions and all angles of all rock slice samples (8) are photographed; The step S2 comprises: 1) placing the rock thin section samples (8) to be tested into the sample placement slot (304) on the sample stage module (3), wherein the number of the rock thin section samples (8) is 4 to 6, and the samples are placed side by side; 2) The computer controls the polarizer module (1) to move horizontally so that the polarizer is inserted; the light emitted by the light source is converted into linearly polarized light after passing through the polarizer, and the linearly polarized light passes through the rock slice sample (8) and enters the microscope eyepiece (6), and then passes through the polarizer to reach the imaging module of the microscope, ensuring that the polarizer and the analyzer are in an orthogonal relationship; the computer controls the sample stage module (3) to move horizontally so that the upper left corner of the rock slice sample (8) appears in the field of view of the imaging module; The computer automatically focuses based on the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image; 3) The computer controls the analyzer and the polarizer to rotate to the initial angle to ensure that the polarizer and the analyzer are in an orthogonal relationship. At the same time, the imaging module takes the first single polarized image, and then controls the polarizer to rotate at a fixed angle interval to ensure that the polarizer and the analyzer are in an orthogonal relationship. Multiple orthogonal polarized images are taken continuously; the taken images are stored in the database; 4) The computer controls the movement of the sample stage module (3) according to the magnification, so that the imaging module captures adjacent images of the rock thin section microscopic images, and the overlapping rate of adjacent rock thin section microscopic images is not less than 20%; then the polarizer is controlled to rotate at a fixed angle interval to ensure that the polarizer and the analyzer are in an orthogonal relationship, and a plurality of orthogonal polarization images are continuously captured; the captured images are stored in a database; the sample stage module (3) continues to move until the first rock thin section completes the capture of all angles in all positions; 5) The computer controls the movement of the sample stage module (3) so that the second rock slice moves into the field of view, and the above steps 2) to 4) are repeated, and so on, until all positions and all angles of all rock slice samples (8) are photographed; The step S3 comprises: 1) placing the rock thin section samples (8) to be tested into the sample placement slot (304) on the sample stage module (3), wherein the number of the rock thin section samples (8) is 4 to 6, and the samples are placed side by side; 2) The computer controls the polarizer module (1) to move horizontally, so that the polarizer is pulled out; the light emitted by the fluorescence module is reflected by the spectroscope (9) and reaches the rock thin section sample (8), thereby stimulating the rock thin section sample (8) to emit fluorescence; the fluorescence emitted by the rock thin section sample (8) enters the microscope eyepiece (6) and then reaches the imaging module of the microscope; the computer controls the sample stage module (3) to move horizontally, so that the upper left corner of the rock thin section sample (8) appears in the field of view of the imaging module; The computer automatically focuses based on the real-time image of the imaging module to ensure that the imaging module can form a clear microscopic image; 3) The computer controls the movement of the sample stage module (3) according to the magnification, so that the imaging module captures adjacent images of the rock thin section microscopic images, and the overlapping rate of adjacent rock thin section microscopic images is not less than 20%; a plurality of fluorescent images are continuously captured, and the captured images are stored in a database; the sample stage module (3) continues to move until the first rock thin section completes the capture of all positions; 4) The computer controls the movement of the sample stage module (3) so that the second rock slice moves into the field of view, and the above steps 2) to 3) are repeated, and so on, until all positions of all rock slice samples (8) are photographed; The step S4 comprises: 1) The computer numbers each single polarization microscopic image, orthogonal polarization microscopic image, and fluorescence microscopic image taken; 2) The computer performs image preprocessing on adjacent single polarized light microscopic images, and then performs image stitching, and stitches the single polarized light microscopic images corresponding to the same rock thin section sample (8) into a complete high-definition large image; the computer performs image preprocessing on adjacent orthogonal polarized light microscopic images, and then performs image stitching, and stitches the orthogonal polarized light microscopic images corresponding to the same rock thin section sample (8) into a complete high-definition large image; the computer performs image preprocessing on adjacent fluorescence microscopic images, and then performs image stitching, and stitches the fluorescence microscopic images corresponding to the same rock thin section sample (8) into a complete high-definition large image; 3) The computer stores the single polarization microscopic image, orthogonal polarization microscopic image and fluorescence microscopic image of the same rock thin section sample (8) as a group in a database; when the computer calls an image from the database, a thumbnail of the high-definition large image is displayed on the computer screen, and a selection box is displayed on the thumbnail, and the high-definition image of the position selected by the selection box is displayed in the center of the screen; drag the selection box, and the high-definition image displayed on the screen moves accordingly, so that convenient observation is achieved.
8. The multi-dimensional rock thin section digital automatic acquisition method according to claim 6, It is characterized in that Image stitching includes: Image Processing: First, randomly select 10-20 pixels in each captured image, and then calculate the median value of the brightness channel of the selected pixels; then obtain the average value of the brightness median value of all images to be stitched; Then the brightness of all images is averaged, that is, the brightness of each image is adjusted so that the median brightness value of each image is equal to the average of the median brightness values of all images; Image Segmentation: Divide each image to be stitched into 10,000 sub-images, and then calculate the texture features of each sub-image; Then, the texture features of the edge sub-images of the adjacent images to be stitched are compared, and the sub-images with the same texture features are marked as the same sub-images; Image stitching: The adjacent images to be stitched are stitched using the marked identical sub-images in the adjacent images to be stitched as the alignment reference; and so on, the stitching of all the images to be stitched is completed to obtain a large field of view stitched image.
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