A device for in-situ detection of key components in tobacco leaves

Abstract

This invention discloses a device for in-situ collection and detection of key components in tobacco leaves, comprising: a light-shielding cover (1); a base plate (14); a flat plate (11); and a dark box (12). The flat plate (11) is sandwiched between the light-shielding cover (1) and the dark box (12). The left side of the light-shielding cover (1) is connected to a spring (2) via a splicing interface (3) and to the dark box (12). An electrochemical workstation (16) is connected to the screen-printed electrode (13). This invention also discloses a method for in-situ collection and detection of key components in tobacco leaves using the aforementioned device. The device of this invention has a simple structure, is easy to operate, and automates and speeds up collection and detection, thus reducing damage to tobacco leaves.

Description

Technical Field

[0001] This invention relates to a device for collecting and detecting trace amounts of sap from live tobacco leaves, and more particularly to a device for in-situ detection of key components in live tobacco leaves. Background Technology

[0002] Plant growth is a complex physiological, biochemical, and metabolic process, regulated by internal and external substances. It manifests in effects on growth vitality, morphological characteristics, cell tissue, and metabolism, directly or indirectly influencing vegetative and reproductive growth, ultimately affecting yield, nutritional value, and quality. Therefore, many agronomists, breeders, and biochemists specializing in tobacco are highly concerned with changes in components such as potassium, nicotine, total sugar, and propylene glycol within tobacco plants. While in vitro plant monitoring techniques are relatively mature, due to significant variations in in vitro environments, rapid electrochemical in vivo detection is gradually becoming a research hotspot. Most existing electrochemical in vivo detection methods utilize screen-printed electrodes (such as...). Figure 1 As shown, sampling devices for detection electrodes are mostly pressure extraction and needle tube aspiration techniques, which can be used for on-site collection, but there is still a lack of devices or instruments suitable for the detection of screen printing electrodes.

[0003] This invention is proposed for this purpose. Summary of the Invention

[0004] To enable in-situ live detection of components within tobacco leaves, it is necessary to collect trace amounts of sap from the leaves. This invention provides a device for in-situ live detection of key components in tobacco leaves. The device is simple in structure, easy to manufacture, and convenient to operate, enabling in-situ live collection and detection of trace amounts of sap from tobacco leaves, thus ensuring the electrochemical live detection of key components within tobacco leaves.

[0005] The technical solution of the present invention is as follows:

[0006] The first aspect of this invention discloses a device for in-situ collection and detection of key components in tobacco leaves, comprising:

[0007] The light-shielding cover 1 is located at the top of the device and is used for light blocking and pressing.

[0008] A screen-printed electrode 13 is placed on the right surface of a substrate 14.

[0009] The plate 11 has a peristaltic pump 5 placed on its upper right side and a pressing plate 7 at its right bottom. A capillary tube 8 perpendicular to the base plate 14 is provided at the bottom of the pressing plate 7. The peristaltic pump 5 is connected to a liquid output tube 6 that extends into the dark box. The peristaltic pump 5 can store and automatically add detection solution for subsequent detection.

[0010] The dark box 12 is rectangular in shape, and there is a notch 121 on the right side of its upper wall that is adapted to the capillary tube 8;

[0011] The flat plate 11 is sandwiched between the light-shielding cover 1 and the dark box 12. The left side of the light-shielding cover 1 is connected to the spring 2 and the dark box 12 through the splicing interface 3. The flat plate 11, the dark box 12 and the base plate 14 are connected by a screw rod 4 located slightly to the left of the middle.

[0012] The opening of the capillary tube 8 corresponds to the electrode working area 15 of the screen printing electrode 13; a liquid output tube 6 extends from the bottom of the peristaltic pump 5, and the opening of the liquid output tube 6 is aligned with the electrode working area 15; the liquid output tube 6 is in the dark box 12.

[0013] An electrochemical workstation 16 is connected to the screen-printed electrode 13.

[0014] Preferably, the dark box 12 is equipped with constant temperature, sealing and light protection to maintain the sampling environment to the greatest extent and prevent the decomposition of the detected components.

[0015] Preferably, there are multiple capillaries 8, which utilize capillary action to make the juice flow out more easily; the area enclosed by the multiple capillaries 8 corresponds to the electrode working area 15 of the screen printing electrode 13.

[0016] Preferably, the upper end of the capillary tube 8 has an air blowing device.

[0017] Preferably, the upper and middle sections of the screw rod 4 are smooth and without threads, so that the light shield 1, the flat plate 11 and the dark box 12 can move up and down to a certain extent when pressed, thus completing manual pressing sampling.

[0018] Preferably, the substrate 14 is provided with a storage box 10 for storing the screen-printed electrodes 13, and the storage box 10 is connected to the button 9 on the cassette 12. The storage box 10 can store and output the screen-printed electrodes 13. By pressing the button 9, the old electrode can be pushed out, and the new modified screen-printed electrode can be manually fixed in a certain position, that is, below the capillary.

[0019] Preferably, the connectors connecting the screen-printed electrode 13 to the electrochemical workstation 16 are respectively the counter electrode 131, the working electrode 132, and the reference electrode 133.

[0020] The second aspect of this invention discloses a method for in-situ collection and detection of key components in tobacco leaves using the aforementioned device, comprising the following steps:

[0021] Connect the light-shielding cover 1, plate 11, dark box 12 and base plate 14, place the new screen-printed electrode 13, and prepare the solution in the peristaltic pump 5.

[0022] Place the part of the fresh tobacco leaf to be collected in the electrode working area 15; press the light shield 1, which causes the plate 11 and the dark box 12 to press down, which in turn causes the pressing plate 7 and the capillary tube 8 to press down. The capillary tube 8 is pressed down from the notch 121 of the dark box 12 towards the part of the fresh tobacco leaf to be collected, and the juice of the fresh tobacco leaf enters the capillary tube 8. Remove the fresh tobacco leaf; use the air blowing device at the upper end of the capillary tube 8 to blow the juice in the capillary tube 8 into the electrode working area 15, and transport the solution in the peristaltic pump 5 to the electrode working area 15 through the port of the liquid output pipe 6.

[0023] By connecting the electrochemical workstation 16 to the connectors of the counter electrode 131, working electrode 132, and reference electrode 133 of the screen-printed electrode 13, key components in live tobacco leaves can be detected in situ.

[0024] The beneficial effects of this invention are:

[0025] This invention discloses a device for in-situ collection and detection of key components in tobacco leaves, enabling the on-site collection and detection of trace amounts of sap from live tobacco leaves. Inspired by the shape and structure of a stapler, the device is simple in structure, easy to manufacture, and convenient to operate. It allows for on-site, in-situ collection and detection of trace amounts of sap from tobacco leaves, avoiding resource waste caused by delayed later detection. This provides assurance for the electrochemical in-situ detection of key components within tobacco leaves and meets the needs of the tobacco industry's testing market. Attached Figure Description

[0026] Figure 1 This refers to the screen-printed electrode of the existing technology.

[0027] Figure 2 This is a schematic diagram of the device for in-situ collection and detection of key components in tobacco leaves according to the present invention.

[0028] Figure 3 This is a schematic diagram of the light-shielding cover of the present invention.

[0029] Figure 4 This is a schematic diagram of the flat panel of the present invention.

[0030] Figure 5 This is a schematic diagram of the dark box of the present invention.

[0031] Figure 6 This is a schematic diagram of the substrate of the present invention.

[0032] Figure 7 This is a schematic diagram of an electrochemical workstation.

[0033] The attached diagram is labeled as follows: 1. Light-shielding cover; 2. Spring; 3. Joint; 4. Screw rod; 5. Peristaltic pump; 6. Liquid output tube; 7. Press plate; 8. Capillary tube; 9. Button; 10. Storage box; 11. Flat plate; 12. Dark box; 121. Notch; 13. Screen-printed electrode; 14. Substrate; 15. Electrode working area; 16. Electrochemical workstation. Detailed Implementation

[0034] The present invention will be further described in detail below with reference to the embodiments and accompanying drawings. The embodiments are only for illustrating the present invention and are not intended to limit the present invention.

[0035] like Figure 2 As shown, the present invention provides an apparatus for in-situ collection and detection of key components in tobacco leaves, comprising: a light-shielding cover 1, as shown in the figure. Figure 3 As shown, located at the top of the device, it is used for light shielding and pressing. Base plate 14, as... Figure 6 As shown, a screen-printed electrode 13 is placed on its right surface. A peristaltic pump 5 is placed on the upper right side of a flat plate 11, and a pressing plate 7 is located at its right bottom. A capillary tube 8 perpendicular to the base plate 14 is located at the bottom of the pressing plate 7. The peristaltic pump 5 is connected to a liquid output tube 6 that extends into the dark box. The peristaltic pump 5 can store and automatically add detection solution for subsequent detection, such as... Figure 4 As shown. Dark box 12, as... Figure 5 As shown, it is a closed cuboid shape with a notch 121 on its right side that is adapted to the capillary tube 8.

[0036] As shown in Figure 2, the plate 11 is sandwiched between the light-shielding cover 1 and the dark box 12. The left side of the light-shielding cover 1 is connected to the spring 2 via the splicing interface 3 and then to the dark box 12. The plate 11, the dark box 12, and the base plate 14 are connected by a screw rod 4 located slightly to the left of the center. The opening of the capillary tube 8 corresponds to the electrode working area 15 of the screen-printed electrode 13. A liquid output tube 6 extends from the bottom of the peristaltic pump 5, and the opening of the liquid output tube 6 is aligned with the electrode working area 15. The liquid output tube 6 is located within the dark box 12. An electrochemical workstation 16, as shown... Figure 7As shown, it is connected to the screen-printed electrode 13. The dark box 12 is designed for constant temperature, sealing, and light shielding; it maintains the sampling environment to the greatest extent possible and prevents decomposition of the detected components. Multiple capillaries 8 are used to facilitate the flow of liquid through capillary action; the area enclosed by the multiple capillaries 8 corresponds to the electrode working area 15 of the screen-printed electrode 13. The upper end of each capillary 8 has an air blowing device. The upper middle section of the screw rod 4 is smooth and threadless; this allows the light shield 1, the plate 11, and the dark box 12 to move up and down slightly when pressed, enabling manual pressing sampling. The base plate 14 is equipped with a storage box 10 for storing the screen-printed electrode 13, which is connected to a button 9 on the dark box 12. The storage box 10 can store and release the screen-printed electrode 13. By pressing button 9, the old electrode is pushed out, and the newly modified screen-printed electrode is manually fixed below the capillaries. The connectors connecting the screen-printed electrode 13 to the electrochemical workstation 16 are respectively the counter electrode 131, the working electrode 132, and the reference electrode 133.

[0037] The method for in-situ collection and detection of key components in tobacco leaves using the apparatus of the present invention includes the following steps:

[0038] Connect the light-shielding cover 1, plate 11, dark box 12 and base plate 14, place the new screen-printed electrode 13, and prepare the solution in the peristaltic pump 5.

[0039] Reference Figure 2 Place the part of the fresh tobacco leaf to be collected in the electrode working area 15; press the light shield 1, which causes the plate 11 and the dark box 12 to press down, which in turn causes the pressing plate 7 and the capillary tube 8 to press down. The capillary tube 8 is pressed down from the notch 121 of the dark box 12 towards the part of the fresh tobacco leaf to be collected, and the juice of the fresh tobacco leaf enters the capillary tube 8. Remove the fresh tobacco leaf; use the air blowing device at the upper end of the capillary tube 8 to blow the juice in the capillary tube 8 into the electrode working area 15, and transport the solution in the peristaltic pump 5 to the electrode working area 15 through the port of the liquid output pipe 6.

[0040] By connecting the electrochemical workstation 16 to the connectors of the counter electrode 131, working electrode 132, and reference electrode 133 of the screen-printed electrode 13, key components in live tobacco leaves can be detected in situ; key components may include potassium, nicotine, total sugar, propylene glycol, etc.

[0041] The above-described embodiments are merely one implementation of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of protection of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the appended claims.

Claims

1. A device for in-situ collection and detection of key components in tobacco leaves, characterized in that, It includes: A light-shielding cover (1) is located at the top of the device and is used for light blocking and pressing; A substrate (14) has a screen-printed electrode (13) placed on its right surface; A flat plate (11) has a peristaltic pump (5) placed on its upper right side and a pressing plate (7) at its right bottom. A capillary tube (8) perpendicular to the base plate (14) is provided at the bottom of the pressing plate (7). The dark box (12) is rectangular in shape, and has a notch (121) on the right side of its upper wall that fits the capillary tube (8); The flat plate (11) is sandwiched between the light-shielding cover (1) and the dark box (12). The left side of the light-shielding cover (1) is connected to the spring (2) through the splicing interface (3) and the dark box (12). The flat plate (11), the dark box (12) and the base plate (14) are connected by a screw rod (4) located slightly to the left of the middle. The opening of the capillary tube (8) corresponds to the electrode working area (15) of the screen printing electrode (13); the bottom of the peristaltic pump (5) has a liquid output tube (6) extending out, and the opening of the liquid output tube (6) is aligned with the electrode working area (15). An electrochemical workstation (16) is connected to the screen-printed electrode (13); There are multiple capillaries (8), and the area enclosed by the multiple capillaries (8) corresponds to the electrode working area (15) of the screen printing electrode (13). The upper end of the capillary (8) is equipped with an air blowing device; The connectors connecting the screen-printed electrode (13) to the electrochemical workstation (16) are the counter electrode (131), the working electrode (132), and the reference electrode (133), respectively.

2. The apparatus according to claim 1, characterized in that, The dark box (12) is equipped with constant temperature, sealing and light-blocking features.

3. The apparatus according to claim 1, characterized in that, The upper and middle sections of the screw rod (4) are smooth and without threads.

4. The apparatus according to claim 1, characterized in that, The substrate (14) is provided with a storage box (10) for storing the screen-printed electrode (13), and the storage box (10) is connected to a button (9) on the dark box (12).

5. The method for in-situ collection and detection of key components in tobacco leaves using the apparatus according to claim 1, characterized in that, Includes the following steps: Connect the light shield (1), plate (11), dark box (12) and base plate (14), place the new screen-printed electrode (13) in place, and prepare the solution in the peristaltic pump (5); Place the part of the fresh tobacco leaf to be collected in the electrode working area (15); press the light shield (1), the light shield (1) drives the plate (11) and the dark box (12) to press down, and drives the pressing plate (7) and the capillary tube (8) to press down. The capillary tube (8) presses down from the notch (121) of the dark box (12) towards the part of the fresh tobacco leaf to be collected. The juice of the fresh tobacco leaf enters the capillary tube (8). Remove the fresh tobacco leaf; use the air blowing device at the upper end of the capillary tube (8) to blow the juice in the capillary tube (8) into the electrode working area (15). The solution in the peristaltic pump (5) is transported to the electrode working area (15) through the port of the liquid output tube (6). By connecting the electrochemical workstation (16) to the connectors of the counter electrode (131), working electrode (132), and reference electrode (133) of the screen-printed electrode (13), key components in live tobacco leaves can be detected in situ.

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