A method, device, system and medium for precise preparation of cell fluid

Abstract

The present invention relates to the technical field of cell preparations, and in particular to a method, device, system and medium for precise preparation of cell fluids. The present application discloses a method for precise preparation of cell fluids, comprising the following steps: obtaining the number of sub-packaging bags and the total volume to be packed; determining the sub-packaging volume corresponding to each sub-packaging bag based on the number of sub-packaging bags and the total volume to be packed; performing preparation processing of the cell fluid on each sub-packaging bag based on the sub-packaging volume corresponding to each sub-packaging bag, and pre-processing the data collected by the weighing sensor during the preparation process to monitor the actual preparation volume of each sub-packaging bag, and making the actual preparation volume of each sub-packaging bag differ from the corresponding sub-packaging volume by a preset difference. The present invention improves the accuracy and correctness of weight collection by utilizing the pre-processing of the weighing sensor.

Description

Technical Field

[0001] The present invention relates to the technical field of cell preparations, and in particular to a method, device, system and medium for precise preparation of cell fluids. Background Art

[0002] Cell therapy is currently a key area of ​​development at the forefront of international medicine. In recent years, new research findings have been continuously made in this field, and the development and evaluation of cell therapy products have received increasing attention from domestic pharmaceutical companies and government departments. Given the current rapid development of cell therapy research, the use of automated cell harvesting equipment is becoming a mainstream development direction.

[0003] Current cell processing requires packaging cells into preparations of varying sizes. Even slight changes in preparation volume can significantly impact the concentration and cell yield of the preparation, placing high demands on the accuracy of the preparation. The harvest weight of existing cell preparation packaging processes can deviate from the set target weight, severely impacting the accuracy, precision, and cell yield of the preparation.

[0004] Therefore, it is necessary to provide an error elimination method that can reduce errors in the packaging process and ensure the quality and accuracy of the preparation. Summary of the Invention

[0005] In order to overcome the shortcomings of the prior art, the present invention provides a method, device, system and medium for the precise preparation of cell fluid. The precise preparation method of cell fluid described in this application improves the accuracy and precision of the packaged preparation through the mutual cooperation of flow sensors, weighing sensors, peristaltic pumps, bubble removers and bubble sensors.

[0006] The technical solution adopted by the present invention to solve its technical problem is:

[0007] A method for precise preparation of cell fluid, comprising:

[0008] Get the number of bags to be packed and the total volume to be packed;

[0009] Determining the packaging volume corresponding to each packaging bag according to the number of packaging bags and the total volume to be packaged;

[0010] Based on the packaging volume corresponding to each packaging bag, the cell solution is prepared for each packaging bag, and the data collected by the weighing sensor during the preparation process is pre-processed to monitor the actual preparation volume of each packaging bag, and the actual preparation volume of each packaging bag is different from the corresponding packaging volume by a preset difference;

[0011] The flow-based emptying pipeline processing method ensures that the actual preparation volume of each filling bag reaches the corresponding filling volume.

[0012] As an improvement to the above technical solution, the data collected by the weighing sensor during the preparation process is preprocessed, including:

[0013] Obtaining a current collection value collected at a current time point and a historical collection set within a preset time period from the current time point; the historical collection set includes each historical time point in the preset time period from the current time point, and the historical collection value and historical true value corresponding to each historical time point; the current collection value and the historical collection value are obtained by collecting the weighing sensor;

[0014] Performing a pendulum test on the current collected value and all the historical collected values ​​to obtain a pendulum test result; the pendulum test result includes a swing period; the pendulum test result indicates whether the packaging bag performs a pendulum motion;

[0015] When it is detected that the pendulum detection result is characterized by pendulum motion, a current real value corresponding to the current time point is determined according to the swing period, the current time point and the current collected value, and a historical collection set is updated.

[0016] As a further improvement of the above technical solution, determining the current true value corresponding to the current time point according to the swing period, the current time point and the current collected value includes:

[0017] Determining a half-cycle time point according to the swing cycle and the current time point;

[0018] Obtaining the historical collected value and the historical true value corresponding to the half-cycle time point;

[0019] A current true value corresponding to the current time point is determined according to the current collected value, the acquired historical collected value, and the historical true value.

[0020] As an improvement to the above technical solution, the flow-based emptying pipeline processing method, which enables the actual preparation volume of each filling bag to reach the corresponding filling volume, includes:

[0021] The capacity of the emptying pipeline of each sub-packaging bag is monitored by a flow sensor to reach the preset difference value corresponding to the sub-packaging bag, so that the actual preparation volume of each sub-packaging bag reaches the corresponding sub-packaging volume.

[0022] As a further improvement of the above technical solution, the flow sensor is provided with a bubble remover in a direction away from the packaging bag.

[0023] As an improvement to the above technical solution, the flow-based emptying pipeline processing method, which enables the actual preparation volume of each filling bag to reach the corresponding filling volume, also includes:

[0024] Obtaining an actual flow rate through the flow-free periods recorded by the first flow sensor and the second flow sensor, and the pipeline paths between the first flow sensor and the second flow sensor;

[0025] According to the length of the pipeline from the one-to-many bifurcated interface to each sub-packaging bag, the preset difference corresponding to each sub-packaging bag and the actual flow rate obtained, the pipeline of each sub-packaging bag is emptied so that the actual preparation volume of each sub-packaging bag reaches the corresponding sub-packaging volume.

[0026] As a further improvement of the above technical solution, before the flow-free period recorded by the first flow sensor and the second flow sensor, the method includes:

[0027] The moment when the cell fluid is evacuated and flows through the first flow sensor is recorded as the first flow empty moment, and the moment when the cell fluid is evacuated and flows through the second flow sensor is recorded as the second flow empty moment; wherein, the first flow sensor is located away from the bifurcation interface; and the second flow sensor is located close to the bifurcation interface;

[0028] The difference between the second flow-free time and the first flow-free time is recorded as the flow-free period.

[0029] A cell fluid precision preparation device, comprising:

[0030] An acquisition module is used to obtain the number of bags to be packed and the total volume to be packed;

[0031] A determination module, configured to determine the packaging volume corresponding to each packaging bag according to the number of packaging bags and the total volume to be packaged;

[0032] A monitoring module is used to perform preparation of the cell solution on each sub-packaging bag based on the sub-packaging volume corresponding to each sub-packaging bag, and pre-process the data collected by the weighing sensor during the preparation process to monitor the actual preparation volume of each sub-packaging bag, and to ensure that the actual preparation volume of each sub-packaging bag differs from the corresponding sub-packaging volume by a preset difference;

[0033] The emptying module is used for a flow-based emptying pipeline processing method so that the actual preparation volume of each filling bag reaches the corresponding filling volume.

[0034] A cell solution precision preparation system, comprising an acquisition module for acquiring the number of packaging bags and the total volume to be packaged;

[0035] A determination module for determining the packaging volume corresponding to each packaging bag according to the number of packaging bags and the total volume to be packaged;

[0036] A monitoring module for performing preparation of cell fluid on each sub-packaging bag based on the sub-packaging volume corresponding to each sub-packaging bag, and pre-processing data collected by the weighing sensor during the preparation process to monitor the actual preparation volume of each sub-packaging bag, and ensuring that the actual preparation volume of each sub-packaging bag differs from the corresponding sub-packaging volume by a preset difference;

[0037] An emptying module for a flow-based emptying pipeline processing method, which ensures that the actual preparation volume of each filling bag reaches the corresponding filling volume;

[0038] And a controller for executing the cell fluid precision preparation method as described above.

[0039] A computer-readable storage medium stores a computer program, which, when executed by a processor, implements the above-mentioned method for precise preparation of cell fluid.

[0040] The beneficial effects of the present invention are:

[0041] 1. The present invention improves the accuracy and correctness of weight collection by utilizing the preprocessing of the weighing sensor;

[0042] 2. The present invention improves the accuracy and precision of the preparation by using a flow sensor to accurately measure the volume of the liquid flowing into the packaging bag;

[0043] 3. The present invention improves the accuracy of emptying the pipeline by utilizing special control of the emptying time of the pipeline;

[0044] 4. Therefore, the present invention improves the accuracy and precision of the preparation by utilizing the information fusion of the flow sensor, weighing sensor, peristaltic pump, and bubble remover, which can reduce the error in the preparation packaging process and ensure the quality and accuracy of the preparation. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] The present invention will be further described below with reference to the accompanying drawings and examples.

[0046] Figure 1 This is a flow chart of the method for precise cell fluid preparation according to the present invention;

[0047] Figure 2 Schematic diagram of the application of the cell fluid precision preparation method of the present invention;

[0048] Description of the drawings: 1. Control valve; 2. Hollow cup; 3. Weighing sensor; 4. Hook; 5. Packaging bag; 6. Tube clamp; 7. Peristaltic pump; 8. Bubble remover; 9. First flow sensor; 10. Second flow sensor. DETAILED DESCRIPTION

[0049] In order to facilitate understanding by those skilled in the art, the present invention will be further described below with reference to the embodiments. The contents mentioned in the embodiments are not intended to limit the present invention.

[0050] like Figure 1 or Figure 2 The method for accurately preparing cell sap includes: obtaining the number of sub-packaging bags and the total volume to be packed; determining the sub-packaging volume corresponding to each sub-packaging bag based on the number of sub-packaging bags and the total volume to be packed; performing cell sap preparation processing on each sub-packaging bag based on the sub-packaging volume corresponding to each sub-packaging bag, and pre-processing the data collected by the weighing sensor during the preparation process to monitor the actual preparation volume of each sub-packaging bag, and making the actual preparation volume of each sub-packaging bag differ from the corresponding sub-packaging volume by a preset difference; and a flow-based emptying pipeline processing method to make the actual preparation volume of each sub-packaging bag reach the corresponding sub-packaging volume. Therefore, a method for accurately preparing cell sap precisely controls the actual preparation volume of each sub-packaging bag by pre-processing the data collected by the weighing sensor during the preparation process, and then delivers the actual preparation volume to the sub-packaging bag through a flow-based emptying pipeline processing method.

[0051] Specifically, in one embodiment, the number of packaging bags Y is 10, and the total volume to be packaged V0 is 110 mL, and the packaging volume V1 corresponding to each packaging bag is 10 ml; the density of the liquid to be prepared remains unchanged during the preparation process, so the actual preparation volume of each packaging bag can be monitored by using a weighing sensor.

[0052] In one embodiment, the data collected by the weighing sensor is preprocessed during the preparation process, including: obtaining a current collected value collected at the current time point and a historical collection set within a preset time period from the current time point; the historical collection set includes each historical time point in the preset time period from the current time point, and the historical collected value and historical true value corresponding to each historical time point; the current collected value and the historical collected value are obtained by collecting the weighing sensor;

[0053] It can be understood that by placing the sub-packaging bag 5 and the weighing sensor 3 together using the same hook 4 or other swingable fixing member, the weighing sensor 3 monitors the actual preparation volume of each sub-packaging bag by collecting the weight change of the sub-packaging bag, the preparation time, and the swing angle of the sub-packaging bag during the preparation process;

[0054] At the current time point t (the time point of the collection at this moment) n (t n =0.5s×n), where 0.5s is the interval time of the acquisition rate, which is the same as the current time point t n The corresponding relationship is expressed as (the current time point t n , current collection value F n, current true value G n , swing angle φ n ); within a preset time period such as Δt (t1 = 0.5s, t2 = 1s, ..., t n ), the historical collection set includes {(historical time point t1, historical collection value F1, historical true value G1, swing angle φ1), (historical time point t2, historical collection value F2, historical true value G2, swing angle φ2)...(historical time point t n-1 , historical collection value F n-1 , historical true value G n-1 , swing angle φ n-1 )}, t1 is the initial time point in the preset time period. As time goes by, Δt remains unchanged, t2 will become t1, t n will become t n-1 ; Among them, the swing angle φ n =arccos(G n / Fn ).

[0055] Performing a pendulum test on the current collected value and all the historical collected values ​​to obtain a pendulum test result; the pendulum test result includes a swing period; the pendulum test result indicates whether the packaging bag performs a pendulum motion;

[0056] It can be understood that the basis for the pendulum detection is: whether the current collected value and all historical collected values ​​have a periodic pattern of changing from small to large and from large to small, or the distribution consistency and periodicity of the swing angle change amplitude values ​​corresponding to the current collected value and all historical collected values, so as to detect whether it has the relevant characteristics of a pendulum. The relevant characteristics include the swing period, which is the period of the regular pattern of changing from small to large and from large to small. If it has the relevant characteristics of a pendulum, it is judged that the pendulum detection result is that the packaging bag is performing a pendulum motion, otherwise it is not a pendulum motion.

[0057] When it is detected that the pendulum detection result is characterized by pendulum motion, a current real value corresponding to the current time point is determined according to the swing period, the current time point and the current collected value, and a historical collection set is updated.

[0058] Furthermore, determining a current real value corresponding to the current time point according to the swing period, the current time point, and the current collected value includes:

[0059] A half-cycle time point is determined according to the swing cycle and the current time point.

[0060] It can be understood that according to the swing period T z and the current time point t n , determine the half-cycle time point as t h＝ tn -(T z / 2).

[0061] The historical collected value and the historical true value corresponding to the half-cycle time point are obtained.

[0062] Understandably, the half-cycle time point t is found from the historical collection set. h The corresponding historical collection value F h and the historical true value G h .

[0063] A current true value corresponding to the current time point is determined according to the current collected value, the acquired historical collected value, and the historical true value.

[0064] Understandably, according to the symmetry of the pendulum motion, the swing angle of the current collected value can be symmetrically calculated as arccos(G h / F h ), determine that the current true value corresponding to the current time point is the product of the current acquisition value and the cosine value of the swing angle, that is, the current true value is G n =F n ×(G h / F h ).

[0065] In one embodiment, the flow-based emptying pipeline processing method, which enables the actual preparation volume of each filling bag to reach the corresponding filling volume, includes:

[0066] The capacity of the emptying pipeline of each sub-packaging bag is monitored by a flow sensor to reach the preset difference value corresponding to the sub-packaging bag, so that the actual preparation volume of each sub-packaging bag reaches the corresponding sub-packaging volume.

[0067] It is understandable that the preset difference can be set to ±3%. When the flow sensor monitors that the capacity of the emptying pipeline of each sub-packaging bag reaches a difference of ±3% of the volume of the preparation corresponding to the sub-packaging bag, the delivery of the preparation is stopped.

[0068] For example, when the packaging volume V1 corresponding to each packaging bag is 10 ml, first monitor the packaging volume in the packaging bag during the preparation process (for example, when the cell fluid reaches 9 ml), and then monitor the capacity of the emptying pipeline through the flow sensor until it reaches 1 ml, stop transporting the cell fluid, and then empty the cell fluid in the emptying pipeline into the packaging bag.

[0069] In one embodiment, the flow sensor is provided with a bubble remover 8 in a direction away from the packaging bag 5 .

[0070] It is understandable that bubbles will be generated when the cell fluid is transported through the pipeline. After the bubbles pass through the flow sensor, there will be errors in the actual volume of cell fluid reaching the filling bag. Therefore, by setting up a bubble remover, the bubbles generated during the cell transportation process can be removed, which facilitates the flow sensor to accurately monitor the capacity of the filling bag emptying pipeline.

[0071] Specifically, a flow sensor is introduced and added at the position of the control valve 1 near the inlet of the sub-packaging bag. The flow sensor can be inserted into the position between the bifurcation of the sub-packaging bag and the control valve 1. The flow sensor can monitor the flow volume. A bubble remover 8 is added before the flow sensor to make the liquid flowing through the flow sensor a bubble-free liquid, thereby increasing the accuracy of the flow sensor in measuring the flow rate. Whenever the volume flowing through the flow sensor exceeds a preset range of the packaging volume of a sub-packaging bag (or does not exceed a preset difference in the packaging volume of the sub-packaging bag), the control valve is closed and the packaging process of the next sub-packaging bag is switched, and the flow volume is also monitored by the flow sensor.

[0072] In another embodiment, the flow-based emptying pipeline processing method, which enables the actual preparation volume of each filling bag to reach the corresponding filling volume, further includes:

[0073] Obtaining an actual flow rate through the flow-free periods recorded by the first flow sensor and the second flow sensor, and the pipeline paths between the first flow sensor and the second flow sensor;

[0074] According to the length of the pipeline from the one-to-many bifurcated interface to each sub-packaging bag, the preset difference corresponding to each sub-packaging bag and the actual flow rate obtained, the pipeline of each sub-packaging bag is emptied so that the actual preparation volume of each sub-packaging bag reaches the corresponding sub-packaging volume.

[0075] Specifically, open the control valve 1 required for the pipeline, start the conveying device (peristaltic pump 7), draw the cell fluid out of the hollow cup 2, let the cell fluid pass through the preset flow sensor, and flow into the sub-packaging bag, the sub-packaging bag is Y, and then the cell fluid is sub-packed into each sub-packaging bag; during the sub-packaging process, after the flow is diverted to each sub-packaging bag 5 by closing the tube clamp 6, when it reaches the last sub-packaging bag 5, the flow empty period T recorded by the first flow sensor 9 (set close to the hollow cup 2) and the second flow sensor 10 (set close to the sub-packaging bag 5), as well as the pipeline path L of the first flow sensor 9 and the second flow sensor 10, the actual flow rate is obtained as the pipeline path L divided by the flow empty period T;

[0076] Based on the length of the pipeline from the one-to-many bifurcated interface to each sub-packaging bag, the preset difference corresponding to each sub-packaging bag, and the actual flow rate obtained, the pipeline of each sub-packaging bag is emptied so that the actual preparation volume of each sub-packaging bag reaches the corresponding sub-packaging volume;

[0077] In another embodiment, the flow-free period recorded by the first flow sensor and the second flow sensor includes:

[0078] The moment when the cell fluid is evacuated and flows through the first flow sensor is recorded as the first flow empty moment, and the moment when the cell fluid is evacuated and flows through the second flow sensor is recorded as the second flow empty moment; wherein, the first flow sensor is located away from the bifurcation interface; and the second flow sensor is located close to the bifurcation interface;

[0079] The difference between the second flow-free time and the first flow-free time is recorded as the flow-free period.

[0080] Specifically, when the first flow sensor 9 (arranged near the hollow cup 2) changes from having liquid flowing through to being empty of liquid, the moment T1 is recorded, indicating that the preparation is nearing the end, and the peristaltic pump 7 continues to run. The moment when the cell fluid is evacuated and flows through the second flow sensor 10 (arranged near the filling bag 5) is recorded as the second flow empty moment and recorded as T2; then the flow empty period T is equal to T2 minus T1.

[0081] In one embodiment, a cell fluid precision preparation device is provided, comprising:

[0082] An acquisition module is used to obtain the number of bags to be packed and the total volume to be packed;

[0083] A determination module, configured to determine the packaging volume corresponding to each packaging bag according to the number of packaging bags and the total volume to be packaged;

[0084] A monitoring module is used to perform preparation of the cell solution on each sub-packaging bag based on the sub-packaging volume corresponding to each sub-packaging bag, and pre-process the data collected by the weighing sensor during the preparation process to monitor the actual preparation volume of each sub-packaging bag, and to ensure that the actual preparation volume of each sub-packaging bag differs from the corresponding sub-packaging volume by a preset difference;

[0085] The emptying module is used for a flow-based emptying pipeline processing method so that the actual preparation volume of each filling bag reaches the corresponding filling volume.

[0086] For the specific definition of the cell fluid precision preparation device, please refer to the definition of the cell fluid precision preparation method above, which will not be repeated here. Each module in the above-mentioned cell fluid precision preparation device can be implemented in whole or in part by software, hardware, and a combination thereof. The above-mentioned modules can be embedded in or independent of the processor in the computer device in hardware form, or can be stored in the memory of the computer device in software form, so that the processor can call and execute the operations corresponding to the above modules.

[0087] In one embodiment, a cell solution precision preparation system is provided, comprising an acquisition module for acquiring the number of packaging bags and the total volume to be packaged;

[0088] A determination module for determining the packaging volume corresponding to each packaging bag according to the number of packaging bags and the total volume to be packaged;

[0089] A monitoring module for performing preparation of cell fluid on each sub-packaging bag based on the sub-packaging volume corresponding to each sub-packaging bag, and pre-processing data collected by the weighing sensor during the preparation process to monitor the actual preparation volume of each sub-packaging bag, and ensuring that the actual preparation volume of each sub-packaging bag differs from the corresponding sub-packaging volume by a preset difference;

[0090] An emptying module for a flow-based emptying pipeline processing method, which ensures that the actual preparation volume of each filling bag reaches the corresponding filling volume;

[0091] And a controller for executing the cell fluid precision preparation method as described above.

[0092] Each module in the controller described above may be implemented in whole or in part through software, hardware, or a combination thereof. Each module may be embedded in or independent of a processor in a computer device in the form of hardware, or may be stored in a memory in the computer device in the form of software, so that the processor can call and execute the corresponding operations of each module.

[0093] In one embodiment, a computer-readable storage medium is provided, wherein the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the method for accurately preparing cell fluid in the above embodiment is implemented.

[0094] Those skilled in the art will appreciate that all or part of the processes in the above-mentioned embodiments can be implemented by instructing the relevant hardware through a computer program. The computer program can be stored in a non-volatile computer-readable storage medium. When the computer program is executed, it can include the processes of the embodiments of the above-mentioned methods. Among them, any reference to memory, storage, database or other media used in the embodiments provided in this application can include non-volatile and / or volatile memory. Non-volatile memory can include read-only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM) or flash memory. Volatile memory can include random access memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in various forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous link (Synchlink) DRAM (SLDRAM), memory bus (Rambus) direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM).

[0095] Those skilled in the art will clearly understand that for the sake of convenience and brevity of description, only the division of the above-mentioned functional units and modules is used as an example. In actual applications, the above-mentioned functions can be distributed and completed by different functional units and modules as needed, that is, the internal structure of the device can be divided into different functional units or modules to complete all or part of the functions described above.

[0096] The above embodiments are preferred implementation schemes of the present invention. In addition, the present invention can also be implemented in other ways. Any obvious replacement without departing from the concept of the present invention is within the scope of protection of the present invention.

Claims

1. A method for precise preparation of cell fluid, characterized in that: include: Get the number of bags to be packed and the total volume to be packed; Determining the packaging volume corresponding to each packaging bag according to the number of packaging bags and the total volume to be packaged; Based on the packaging volume corresponding to each packaging bag, the cell solution is prepared for each packaging bag, and the data collected by the weighing sensor during the preparation process is pre-processed to monitor the actual preparation volume of each packaging bag, and the actual preparation volume of each packaging bag is different from the corresponding packaging volume by a preset difference; The emptying pipeline processing method based on flow rate ensures that the actual dosage form volume of each filling bag reaches the corresponding filling volume; The data collected by the weighing sensor is pre-processed during the preparation process, including: Obtaining a current collection value collected at a current time point and a historical collection set within a preset time period from the current time point; the historical collection set includes each historical time point in the preset time period from the current time point, and the historical collection value and historical true value corresponding to each historical time point; the current collection value and the historical collection value are obtained by collecting the weighing sensor; Performing a pendulum test on the current collected value and all the historical collected values ​​to obtain a pendulum test result; the pendulum test result includes a swing period; the pendulum test result indicates whether the packaging bag performs a pendulum motion; When it is detected that the pendulum detection result is characterized by pendulum motion, a current real value corresponding to the current time point is determined according to the swing period, the current time point and the current collected value, and a historical collection set is updated.

2. A method for accurate preparation of cell fluid according to claim 1, characterized in that: The determining, based on the swing period, the current time point, and the current collected value, a current real value corresponding to the current time point includes: Determining a half-cycle time point according to the swing cycle and the current time point; Obtaining the historical collected value and the historical true value corresponding to the half-cycle time point; A current true value corresponding to the current time point is determined according to the current collected value, the acquired historical collected value, and the historical true value.

3. A method for accurate preparation of cell fluid according to claim 1, characterized in that: The flow-based emptying pipeline processing method, which enables the actual preparation volume of each filling bag to reach the corresponding filling volume, includes: The capacity of the emptying pipeline of each sub-packaging bag is monitored by a flow sensor to reach the preset difference value corresponding to the sub-packaging bag, so that the actual preparation volume of each sub-packaging bag reaches the corresponding sub-packaging volume.

4. A method for accurate preparation of cell fluid according to claim 3, characterized in that: The flow sensor is provided with a bubble remover in a direction away from the packaging bag.

5. The method for accurate preparation of cell fluid according to claim 1, characterized in that: The flow-based emptying pipeline processing method, which enables the actual preparation volume of each filling bag to reach the corresponding filling volume, also includes: Obtaining an actual flow rate through the flow-free periods recorded by the first flow sensor and the second flow sensor, and the pipeline paths between the first flow sensor and the second flow sensor; According to the length of the pipeline from the one-to-many bifurcated interface to each sub-packaging bag, the preset difference corresponding to each sub-packaging bag and the actual flow rate obtained, the pipeline of each sub-packaging bag is emptied so that the actual preparation volume of each sub-packaging bag reaches the corresponding sub-packaging volume.

6. A method for accurate preparation of cell fluid according to claim 5, characterized in that: Before the flow-free period recorded by the first flow sensor and the second flow sensor, the method includes: The moment when the cell fluid is evacuated and flows through the first flow sensor is recorded as the first flow empty moment, and the moment when the cell fluid is evacuated and flows through the second flow sensor is recorded as the second flow empty moment; wherein, the first flow sensor is located away from the bifurcation interface; and the second flow sensor is located close to the bifurcation interface; The difference between the second flow-free time and the first flow-free time is recorded as the flow-free period.

7. A cell fluid precision preparation device, characterized in that: The cell fluid precision preparation device is used to implement the cell fluid precision preparation method according to any one of claims 1 to 6, comprising: An acquisition module is used to obtain the number of bags to be packed and the total volume to be packed; A determination module, configured to determine the packaging volume corresponding to each packaging bag according to the number of packaging bags and the total volume to be packaged; A monitoring module is used to perform preparation of the cell solution on each sub-packaging bag based on the sub-packaging volume corresponding to each sub-packaging bag, and pre-process the data collected by the weighing sensor during the preparation process to monitor the actual preparation volume of each sub-packaging bag, and to ensure that the actual preparation volume of each sub-packaging bag differs from the corresponding sub-packaging volume by a preset difference; The emptying module is used for a flow-based emptying pipeline processing method so that the actual preparation volume of each filling bag reaches the corresponding filling volume.

8. A cell fluid precision preparation system, characterized in that: It includes an acquisition module for acquiring the number of bags to be packaged and the total volume to be packaged; A determination module for determining the packaging volume corresponding to each packaging bag according to the number of packaging bags and the total volume to be packaged; A monitoring module for performing preparation of cell fluid on each sub-packaging bag based on the sub-packaging volume corresponding to each sub-packaging bag, and pre-processing data collected by the weighing sensor during the preparation process to monitor the actual preparation volume of each sub-packaging bag, and ensuring that the actual preparation volume of each sub-packaging bag differs from the corresponding sub-packaging volume by a preset difference; An emptying module for a flow-based emptying pipeline processing method, which ensures that the actual preparation volume of each filling bag reaches the corresponding filling volume; And a controller for executing the cell fluid precision preparation method according to any one of claims 1 to 6.

9. A computer-readable storage medium storing a computer program, characterized in that: When the computer program is executed by a processor, the method for precise preparation of cell fluid according to any one of claims 1 to 6 is implemented.

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