An efficient potassium-solubilizing Streptomyces actinomycete and its uses

Abstract

The present invention discloses a potassium-dissolving Streptomyces with high efficiency and its uses. The potassium-dissolving Streptomyces with high efficiency is identified as Streptomyces wuyuanensis ( Streptomyces wuyuanensis ), and the strain name is KLBMP9348, which is deposited in the Guangdong Microbial Culture Collection Center. The deposition date is August 24, 2022, and the deposition number is GDMCC NO: 62737. Compared with the prior art, the potassium-dissolving Streptomyces with high efficiency KLBMP9348 provided by the present invention has good growth characteristics and is easy to culture. It is a multifunctional strain with high potassium-dissolving activity, and also has the characteristics of phosphorus solubilization, plant hormone production and inhibition of plant black spot disease. Therefore, it can be used to prepare products for potassium dissolution, phosphorus solubilization, plant hormone production and inhibition of plant black spot disease, such as microbial fertilizers, etc. It has good comprehensive performance and has good application prospects and market value.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and particularly relates to a high-efficiency potassium-solubilizing active Streptomyces and a use thereof. Background Art

[0002] Element K is often known as a quality-enhancing element for cash crops. Along with N and P, it forms one of the three essential nutrients for sweet potato growth and development. It is a key pillar of agricultural production and is primarily used in the production of potash fertilizers. Potassium enhances the resilience of cash crops to stresses such as pests and diseases, lodging resistance, drought, and cold weather, thereby improving crop yield and quality. Global potassium resources are extremely unevenly distributed, with Canada, Belarus, and Russia boasting the largest potash salt resources. China possesses abundant unconventional potassium resources, with proven reserves of insoluble potassium resources such as alunite and potassium feldspar reaching 300 billion tons. However, China faces a shortage of soluble potash salts. Proven soluble potassium resources are primarily found in salt lake brines, primarily in the Qaidam Basin of Qinghai Province. Potash salt shortages threaten China's resource security and hinder agricultural development. Increased efforts in the conversion, development, and utilization of potassium, along with continued technological research and development by manufacturers and researchers to improve and innovate related potassium extraction processes, could increase domestic potash salt production.

[0003] Microorganisms are the oldest living organisms on Earth, with a history of at least 4.1 billion years. Their interactions with minerals occur extensively throughout Earth's surface lithosphere. Mineral rocks provide microorganisms with energy, micronutrients, and electron acceptors for their metabolic activities. In turn, microbial metabolic activity promotes the dissolution and precipitation of minerals, profoundly impacting the geochemical cycles of numerous inorganic and organic elements and, consequently, the environment. All bacteria capable of weathering potassium-containing minerals are termed potassium-dissolving bacteria. Silicate bacteria are a key group, first isolated directly from soil by researchers Alexandrov and Zak, who discovered their ability to activate potassium in silicate minerals. Since their discovery, various theories have emerged regarding their mechanisms, including acidolysis, exopolysaccharide formation, and the formation of bacteria-mineral complexes. Acidolysis involves the solubilization of minerals by small organic acids such as oxalic acid, citric acid, and tartaric acid, as well as amino acids, and the complexation of organic molecules. The exopolysaccharide pathway involves the weathering of minerals by microbial capsular and exopolysaccharides, disrupting the mineral lattice. The formation of bacteria-mineral complexes occurs when bacterial siderophores, during the weathering of potassium minerals, accelerate the formation of corrosion pits on the mineral surface, chelate metal ions on the mineral surface, disrupt the mineral's lattice structure, and promote mineral decomposition. Some filamentous microorganisms, such as cyanobacteria, actinomycetes, and fungi, can even penetrate minerals and rocks.

[0004] Scholars at home and abroad have successively isolated and obtained many other potassium mineral-dissolving bacteria. The bacterium Burkholderia glathei can accelerate the weathering of biotite minerals, releasing magnesium and potassium that can be absorbed by plants, and has a significant growth-promoting effect on pine trees. Agrobacterium tumefaciens, derived from the rhizosphere of cotton in sandy soil, can also significantly increase the release of potassium. There are a large number of mineral-decomposing bacteria in the mineral soil of the potash mining area, mainly classified into the genera Pantoea, Serratia, and Pseudomonas. Actinomycetes can also weather potassium-containing minerals. For example, Streptomyces BM-2 has nitrogen fixation and organic potassium decomposition activities, and can produce ACC deaminase, siderophores, and IAA. The potassium decomposition rate of Streptomyces shaanxiensis is about 20%. Enterobacter asburiae can significantly increase potassium release, and its application as a bacterial agent in flue-cured tobacco can significantly increase tobacco yield.

[0005] In agriculture, potassium-solubilizing bacteria are primarily used as a primary component of microbial preparations. Their primary functions include improving soil fertility, promoting crop growth, and protecting the ecological environment. Numerous experiments have demonstrated that potassium-solubilizing bacteria can increase the content of available elements in soil and enhance soil enzyme activity. Experimental results by Yang Dongyan et al. showed that the application of potassium-solubilizing bacteria not only significantly increased soil available potassium content but also released phosphorus more effectively than phosphate-solubilizing bacteria. Research by Liu Xiaoqian et al. showed that potassium-solubilizing bacteria treatment increased the activities of four enzymes, catalase, urease, sucrase, and acid phosphatase, by 40.2%, 95.6%, 119.4%, and 29.0%, respectively, compared to the control. Potassium-solubilizing bacteria provide nutrients for crop growth, promote crop growth, and improve crop quality. Experimental results by Xi Beibei et al. showed that potassium-solubilizing bacteria can increase seed germination rate, plant height, fresh weight, and dry weight, and can secrete auxins and proteases, exhibiting antibacterial effects. Arbuscular mycorrhizal fungi (AMF) have been shown to increase the yield of sweet potato varieties such as NASPOT 11. Actinomycete UAE1 promotes the growth of the halophyte Salicornia by increasing free polyamines and other plant growth hormones. Streptomyces improves the NPK absorption and drought resistance of Rhamnus serrata seedlings.

[0006] Among microorganisms used for bio-promotion and pest control, research focuses primarily on phosphorus and nitrogen fixation, while potassium solubilization is less common. Furthermore, research on potassium solubilization in actinomycetes is also limited. Therefore, developing an actinomycete strain that can activate potassium and phosphorus, produce plant hormones, and provide disease resistance is of great significance for soil remediation, promoting plant growth, controlling plant diseases, and ensuring food security. Summary of the Invention

[0007] Purpose of the invention: In view of the problems existing in the prior art, the purpose of the present invention is to provide a high-efficiency potassium-solubilizing active Streptomyces and its use. The strain has high potassium-solubilizing activity, and also has the characteristics of phosphorus solubilization, plant hormone production and inhibition of plant black spot disease, and can be used to prepare microbial fertilizer.

[0008] Technical solution: In order to achieve the above-mentioned purpose of the invention, the technical solution adopted by the present invention is as follows:

[0009] A highly efficient potassium-solubilizing Streptomyces was identified as Streptomyces wuyuanensis, the strain name was KLBMP9348, and it was deposited in the Guangdong Provincial Microbiological Culture Collection Center with a deposit date of August 24, 2022 and a deposit number of GDMCCNO: 62737.

[0010] The highly efficient potassium-solubilizing Streptomyces strain KLBMP9348 was isolated and screened from weathered rock and soil in the potash mining area of ​​Fengxian County, Xuzhou, and subsequently studied and expanded for cultivation. The highly efficient potassium-solubilizing Streptomyces strain KLBMP9348 preserved in the present invention exhibits an off-white colony with a wrinkled, dry, opaque, and powdery surface. Scanning electron microscopy revealed loose spiral chains of KLBMP9348 spores with a smooth surface.

[0011] The 16S gene sequence of the highly efficient potassium-solubilizing Streptomyces KLBMP9348 is shown in SEQ ID NO.1.

[0012] The present invention also provides the use of the high-efficiency potassium-solubilizing active Streptomyces in preparing potassium-solubilizing products.

[0013] The present invention also provides the use of the high-efficiency potassium-solubilizing active Streptomyces in preparing a phosphate-solubilizing product.

[0014] The present invention also provides the use of the high-efficiency potassium-solubilizing active Streptomyces in the preparation of plant hormone-producing products.

[0015] The present invention also provides the use of the high-efficiency potassium-solubilizing active Streptomyces in preparing a product for inhibiting plant black spot disease.

[0016] Preferably, the plant black spot disease is sweet potato black spot disease.

[0017] Preferably, the product is a microbial fertilizer.

[0018] The present invention also provides a composition comprising the highly efficient potassium-solubilizing Streptomyces.

[0019] Finally, the present invention provides the use of the composition in preparing products for dissolving potassium, dissolving phosphorus, producing plant hormones and inhibiting plant black spot disease.

[0020] Beneficial Effects: Compared with existing technologies, the highly efficient potassium-solubilizing Streptomyces KLBMP9348 provided by the present invention exhibits excellent growth characteristics and is easy to cultivate. It is a multifunctional strain with high potassium-solubilizing activity, as well as the ability to solubilize phosphates, produce plant hormones, and inhibit plant black spot disease. Therefore, it can be used to prepare products that solubilize potassium, phosphates, produce plant hormones, and inhibit plant black spot disease, such as microbial fertilizers. It exhibits excellent overall performance and has promising application prospects and market value. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 These are the colony morphology and bacterial morphology of the highly efficient potassium-solubilizing Streptomyces KLBMP9348 described in the present invention on ISP2 culture medium.

[0022] Figure 2 Phylogenetic tree of KLBMP9348 constructed based on 16S rDNA gene sequence

[0023] Figure 3 These are the potassium-solubilizing ability test results and electron microscope images of the highly efficient potassium-solubilizing Streptomyces KLBMP9348 described in the present invention.

[0024] Figure 4 This is the test result of the phosphate solubilization ability of the efficient potassium-solubilizing Streptomyces KLBMP9348 described in the present invention.

[0025] Figure 5 These are the test results of the polyamine production ability of the highly efficient potassium-solubilizing Streptomyces KLBMP9348 described in the present invention.

[0026] Figure 6 This is the test result of the indoleacetic acid production ability of the efficient potassium-solubilizing Streptomyces KLBMP9348 described in the present invention.

[0027] Figure 7 This is a diagram of the efficient potassium-solubilizing Streptomyces KLBMP9348 described in the present invention antagonizing sweet potato black spot pathogen. DETAILED DESCRIPTION

[0028] The present invention is further illustrated by the following examples. These examples are purely illustrative and are intended only to provide a detailed description of the present invention and should not be construed as limiting the present invention. Where specific techniques or conditions are not specified in the examples, the methods were performed in accordance with those described in the literature in the art or in accordance with the product instructions. For reagents or instruments used, for which the manufacturer is not specified, all are conventional products commercially available through regular channels.

[0029] Experimental Example 1

[0030] A high-efficiency potassium-solubilizing Streptomyces wuyuanensis, abbreviated as KLBMP9348, has the functions of solubilizing potassium and phosphorus, producing plant hormones, and inhibiting sweet potato black spot disease. It can be used to prepare microbial fertilizers. The strain was deposited in the Guangdong Provincial Microbiological Culture Collection Center on August 24, 2022, with the deposit number GDMCC NO: 62737. The deposit address is the Institute of Microbiology, Guangdong Academy of Sciences, 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

[0031] Among them, other reagents used in the examples of the present invention are all analytically pure.

[0032] The highly efficient potassium-solubilizing Streptomyces KLBMP9348 of the present invention was isolated from soil in the residual mining area of ​​Fengxian County, Xuzhou City, Jiangsu Province. The specific steps are as follows:

[0033] (1) Screening and culture of strains: After sterile air drying in the laboratory, 2 g of soil sample was placed in a sterile Erlenmeyer flask, dried at 100°C for 1 h, cooled, and 18 mL of sterile water was added and thoroughly mixed to prepare a soil suspension. The soil suspension was diluted 100- and 1000-fold, and 200 μL was aspirated and spread on a plate. The plate was placed upright on a clean bench for 20 min, then inverted in an incubator and cultured at 28°C for 28 days. After 28 days, a single colony was picked and transferred to ISP 2 (medium purification) and cultured at 28°C for 7-14 days.

[0034] (2) Strain preservation:

[0035] a. Glycerol tube storage method: Add 10 mL of glycerol and 40 mL of distilled water to a 100 mL Erlenmeyer flask, shake well, and sterilize. Aseptically aliquot 1 mL per cryotube. Pick a colony, crush the cells against the wall of the cryotube, and dissolve them in the glycerol solution. Store three tubes per strain in cryotubes in sequentially numbered order. Store at -20°C or -80°C.

[0036] b. Skim milk powder freeze drying method:

[0037] Cryopreservation tubes: Soak in 2% hydrochloric acid for 8 hours, rinse with tap water several times, rinse with distilled water twice, dry, plug the tube mouth with a cotton swab, sterilize at 121℃ for 15 minutes, and dry.

[0038] Protective agent: 100 mL of distilled water, preheat to about 80°C, add 15% skim milk powder, mix well, sterilize at 115°C for 15 minutes, and cool for later use.

[0039] Pick a colony into a cryovial, crush it, add 1 mL of protective agent, mix thoroughly, and pipette it to the bottom of the cryovial. Store at 4°C, -20°C for 30 minutes, and -80°C for 8 hours. Freeze-dry in a freeze dryer for 8 hours, melt the tube using an alcohol burner, and seal it. Store at 4°C for long-term storage.

[0040] (3) Morphological and physiological and biochemical characteristics of the strain

[0041] Morphological characteristics: The colony is off-white, with a wrinkled, dry, opaque, and powdery surface. The colony morphology on ISP2 medium is shown in Figure 1 .

[0042] The results of physiological and biochemical characteristics are shown in Table 1:

[0043] Table 1 Physiological and biochemical characteristics of strains

[0044]

[0045]

[0046] Note: “+” means positive; “-” means negative

[0047] (4) DNA extraction of strains

[0048] a. Use a sterile bamboo stick to pick up a small amount of bacteria (try to avoid agar), crush them in a sterile EP tube (use the thick end of the bamboo stick), and store at -20℃.

[0049] b. Reagents:

[0050] Washing solution: 50 mmol / L Tris, pH 7.7, 25 mmol / L EDTA, 0.1% PVP. (Tris 1.21 g, EDTA 1.46 g, PVP 0.2 g, distilled water 200 mL, pH 7.7, sterilized, stored at room temperature.)

[0051] Lysis buffer: 50 mmol / L Tris, pH 8.0, 25 mmol / L EDTA, 3% SDS, 1.2% PVP. (Tris 0.605 g, EDTA 0.73 g, SDS 3 g, PVP 1.2 g, distilled water 100 mL, pH 8.0, sterilized, stored at room temperature.)

[0052] Extraction solution: 10 mmol / L Tris, pH 8.0, 1 mmol / L EDTA, 0.3 mol / L NaAc, 1.2% PVP. (Tris 0.06 g, EDTA 0.0146 g, distilled water 50 mL, pH 8.0, sterilized, stored at room temperature.)

[0053] Phenol:chloroform:isoamyl alcohol = 25:24:1 (prepare for use).

[0054] 70% ethanol (freshly prepared for use).

[0055] c. Extraction step

[0056] ① Add 1 mL of washing solution (containing lysozyme at a final concentration of 2 mg / mL) and shake the flask at 37°C for 1 h.

[0057] ② Centrifuge at 6000 rpm for 2 minutes, discard the supernatant, add 35 μL of lysis buffer, vortex to suspend, and microwave (medium heat) for 45 seconds.

[0058] ③Add 400 μL of extraction solution (preheated at 65°C) and shake for 5 seconds.

[0059] ④ Add 500 μL (equal volume) of phenol:chloroform:isoamyl alcohol (25:24:1) solution for extraction, shake, centrifuge at 10,000 r / min for 5 min, slowly aspirate 200 μL of the supernatant and transfer to a 0.5 mL EP tube.

[0060] ⑤ Add 200 μL (equal volume) of phenol:chloroform:isoamyl alcohol (25:24:1) solution for extraction, shake, centrifuge at 10,000 r / min for 5 min, slowly aspirate 100 μL of the supernatant and transfer to a 0.5 mL EP tube.

[0061] ⑥ Add 100 μL of isopropanol, let it stand at -20°C for 30 min, and centrifuge at 10,000 rpm for 5 min.

[0062] ⑦ Wash with 200 μL of 70% ethanol, centrifuge at 10,000 rpm for 5 minutes, slowly pour off the supernatant, and repeat twice.

[0063] ⑧ Dry in a 65℃ water bath, add 20μL sterile distilled water (for molecular experiments), and store at 4℃.

[0064] (5) Strain identification

[0065] 16S rDNA gene PCR amplification:

[0066] Forward Prime A: 5'-CAGAGTTTGATCCTGGCT-3' (bases 7 to 24 of E. coli)

[0067] Reverse Prime B: 5'-AGGAGGTGATCCAGCCGCA-3' (bases 1540-1522 in E. coli)

[0068] The PCR reaction system is shown in Table 2:

[0069] Table 2 PCR reaction system

[0070]

[0071] The reaction procedure is shown in Table 3:

[0072] Table 3 Reaction schedule

[0073]

[0074] The bacterial 16S rDNA sequence was amplified by PCR, and the amplified product was sequenced and compared with the known 16S rDNA sequences in EzBioCloud, and was identified as Streptomyces.

[0075] Experimental Example 2

[0076] The highly efficient potassium-dissolving Streptomyces KLBMP9348 is used to dissolve potassium feldspar powder, thereby increasing the potassium ion content of the culture solution. The treatment method is as follows:

[0077] (1) Select a single colony from the isolation culture medium and inoculate it into a modified potassium-dissolving medium. The modified potassium-dissolving medium formula is: glucose 5.0g / L, Na2HPO4 2.0g / L, MgSO4·7H2O 0.5g / L, Na2HPO4 2.0g / L, FeCl3 5.0mg / L, CaCO3 0.1g / L, potassium feldspar powder 1.0g / L, agar 20.0g / L, pH 7.0, and 100mg / L BTB colorimetric reagent is added to the culture medium. Pretreatment of potassium feldspar powder: Potassium feldspar powder is sieved through a 1000 mesh sieve, soaked in deionized water overnight, rinsed with 20% hydrochloric acid solution, and then ultrasonically cleaned several times with ultrapure water until the solution pH is neutral. Dry and store.

[0078] (2) Place the inoculated plate upside down in a 28°C incubator for 3-7 days to observe whether a yellow potassium-dissolving zone is formed.

[0079] (3) The Streptomyces after initial screening was made into spore liquid (1×10 8 CFU / mL) and inoculated into the seed solution at a 10% inoculum volume for activation.

[0080] (4) The seed solution was inoculated into Aleksandrov medium at a 2% inoculum size and cultured at 28°C and 180 rpm for 14 days. The Aleksandrov medium formulation was MgSO4·7H2O 0.5 g / L, CaCO3 0.1 g / L, FeCl3 5.0 mg / L, Ca3(PO4)2 2.0 g / L, K-feldspar powder 5.0 g / L, glucose 5.0 g / L, and (NH4)2SO4 5.0 g / L.

[0081] (5) Determine the potassium ion content in the supernatant after culture.

[0082] The results are as follows Figure 3 As shown, the KLBMP9348 of the present invention increases the potassium ion content in the culture medium and has strong practicality. After measurement, the potassium ion content in this example is 13.267 μg / mL.

[0083] Experimental Example 3

[0084] The highly efficient potassium-dissolving Streptomyces KLBMP9348 is used to dissolve inorganic phosphorus and increase the phosphate content of the culture solution. The treatment method is as follows:

[0085] (1) The isolated and purified strain was inoculated onto a modified inorganic phosphate medium (NBRIP) plate using a sterilized bamboo stick. Three stripes of bacterial lawn were streaked onto the plate. After culturing at 28°C for 5-7 days, the plate was observed for the appearance of a clearing zone. The modified NBRIP medium formula was as follows: glucose 5.00 g / L, MgCl·6H2O 5.00 g / L, MgSO4·7H2O 0.25 g / L, (NH4)2SO4 0.10 g / L, KCl 0.20 g / L, FeSO4·7H2O 0.01 g / L, Ca3(PO4)2 5.00 g / L, pH 7.0-7.4, with bromophenol blue as a color developer.

[0086] (2) The test bacteria were inoculated into 50 mL of NBRIP liquid culture medium and cultured at 28°C, 180 rpm for 5 days. The bacterial solution was centrifuged at 10,000 rpm for 10 min, the pH value of the supernatant was measured, 1.25 mL of the supernatant was taken and added to a test tube containing 2.5 mL of molybdenum antimony antimony developer. The test tube was slowly shaken to expel CO2 from the liquid and the volume was made up to 10 mL with deionized water. After shaking, the tube was allowed to stand for 30 min and the color was compared at 730 nm. The measured absorbance value was substituted into the standard curve formula to calculate the phosphate yield.

[0087] The results are as follows Figure 4 As shown, KLBMP9348 of the present invention increases the phosphate concentration in the culture medium. After measurement, the phosphate concentration in this example is 1.572 μg / mL. Therefore, 9348 has the ability to hydrolyze inorganic phosphorus.

[0088] Experimental Example 4

[0089] The highly efficient potassium-solubilizing Streptomyces KLBMP9348 can produce polyamines, and the processing method is as follows:

[0090] (1) Inoculate the strain into 0.2 g / L phenol red-modified Moeller decarboxylase agar medium and incubate at 28°C in the dark for 5-7 days. Observe whether dark red circles form below and around the colonies. Moeller decarboxylase agar medium formula: 5.0 g / L peptone, 3.0 g / L yeast extract, 1.0 g / L glucose, 5.0 mg / L pyridoxal-5-phosphate, 2.0 g / L L-arginine, 15.0 g / L agar, pH 6.5.

[0091] (2) Prepare the strain into a spore suspension (1×108 CFU / mL), inoculate 2 mL of spore suspension into 50 mL of polyamine liquid medium and incubate at 180 rpm at 28°C for 5 days. Transfer 500 μL of supernatant to a 10 mL plastic centrifuge tube with a lid, add 100 μL of benzoyl chloride, and then add 1 mL of 2 mol / L NaOH solution. Vortex for 20 seconds and incubate in a 37°C water bath for 20 minutes. Add 2 mL of saturated NaCl solution, mix thoroughly, extract with 2 mL of ether, and centrifuge (3000 rpm) for 5 minutes. Transfer 1 mL of the ether phase to a 2 mL centrifuge tube, vacuum dry, and dissolve in 1 mL of methanol by vortexing. Pass the filtrate through a 0.45 μm filter, and analyze by high-performance liquid chromatography.

[0092] The results are as follows Figure 5 As shown, the KLBMP9348 of the present invention has a strong ability to produce polyamines and is very practical.

[0093] Experimental Example 5

[0094] The highly efficient potassium-solubilizing Streptomyces KLBMP9348 can produce indoleacetic acid, and the processing method is as follows:

[0095] (1) Nitrogen-containing culture medium: glucose 10.0 g / L, (NH4)2SO4 1.0 g / L, K2HPO4 2.0 g / L, MgSO4·7H2O 0.5 g / L, NaCl 0.1 g / L, yeast extract 0.5 g / L, CaCO3 0.5 g / L, pH 7.2.

[0096] (2) Sackowski's developer: Dissolve 150 mL of concentrated sulfuric acid in 250 mL of deionized water, and add 7.5 mL of 0.5 mol / L FeCl3·6H2O solution.

[0097] (3) Prepare a 2.5 mg / mL tryptophan solution and filter sterilize. Dispense the nitrogen-containing culture medium into test tubes, 4 mL per tube. After autoclaving at 121°C, add 1 mL of the filter-sterilized tryptophan solution to make the Trp concentration 0.5 mg / mL. Pick a strain and inoculate it into the above culture medium. Incubate on a shaker for 4 days. Use a sterile pipette to remove 1 mL of the bacterial solution and place it in a 7 mL sterilized centrifuge tube. Add 2 mL of Sackowski's colorimetric reagent and mix thoroughly. Develop at room temperature for 20 minutes. A pink color indicates positive IAA production.

[0098] The results are as follows Figure 6 As shown, the KLBMP9348 of the present invention can produce indoleacetic acid and has certain practicality.

[0099] Experimental Example 6

[0100] KLBMP9348 was inoculated onto a PDA plate where the sweet potato black spot pathogen had grown, and cultured at 28°C for 3-5 days to observe whether antibacterial activity occurred.

[0101] Depend on Figure 7 It can be seen that KLBMP9348 can antagonize the growth of sweet potato black spot pathogen Ceratocytis fimbriata.

[0102] The applicant states that the present invention is intended to illustrate the detailed methods of the present invention through the above-described embodiments, but the present invention is not limited to the above-described detailed methods, that is, it does not mean that the present invention must rely on the above-described detailed methods in order to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions for various raw materials in the products of the present invention, addition of auxiliary ingredients, and selection of specific methods, etc., are all within the scope of protection and disclosure of the present invention.

Claims

1. An efficient potassium-solubilizing Streptomyces, characterized in that, Identified as Streptomyces wuyuanensis ( Streptomyces wuyuanensis ), the strain is named KLBMP9348, deposited in the Guangdong Provincial Culture Collection of Microorganisms on August 24, 2022, with the deposit number GDMCC NO: 62737.

2. The high-efficiency potassium-dissolving Streptomyces described in claim 1, characterized in that, The 16S gene sequence of the Streptomyces wuyuanensis KLBMP9348 is shown as SEQ ID NO.

1.

3. Use of the highly potassium-dissolving active Streptomyces in the preparation of potassium-dissolving products.

4. Use of the highly potassium-dissolving active Streptomyces in the preparation of phosphorus-dissolving products.

5. Use of the highly potassium-dissolving active Streptomyces in the preparation of products producing polyamines and indoleacetic acid.

6. Use of the highly potassium-dissolving active Streptomyces in the preparation of products for inhibiting sweet potato black rot.

7. The application according to any one of claims 3-6, characterized in that, The product is a microbial fertilizer.

8. A composition comprising the highly potassium-dissolving active Streptomyces according to claim 1.

9. Use of the composition according to claim 8 in the preparation of potassium-dissolving, phosphorus-dissolving, polyamine- and indoleacetic acid-producing, and sweet potato black rot-inhibiting products.

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