Combined sampling device

Abstract

The present invention discloses a combined sample adding device, belonging to the field of medical devices, which includes a combined plate. The combined plate includes a sample plate and a receiving plate. The receiving plate is installed on the sample plate. The sample plate is provided with a sample receiving portion, and the receiving plate is provided with a plurality of receiving holes and a sample injection flow channel. The sample injection flow channel is communicated with the sample receiving portion and the receiving holes. The combined sample adding device further includes a pipetting structure, which is communicated with the plurality of receiving holes. The pipetting structure makes the plurality of receiving holes form a negative pressure, and the liquid sample in the sample receiving portion flows into each receiving hole along the sample injection flow channel respectively. Through the above design, it is possible to add samples to a plurality of receiving holes at one time and avoid the mutual mixing and contamination of the solutions in each receiving hole.

Description

Technical Field

[0001] The present invention relates to the field of medical devices, and more particularly to a combined sampling device. Background Art

[0002] In the field of biomedicine, there are often scenarios where a sample needs to be added to each well of a multi-well plate, while preventing communication and cross-contamination between the wells. The prior art uses disposable pipettes, and the disposable pipette tips need to be replaced between samplings for each well. This is not only time-consuming and laborious, but also increases the consumption of disposable tips. Summary of the Invention

[0003] In order to overcome the deficiencies of the prior art, one of the objectives of the present invention is to provide a combined sampling device that can sample multiple receiving wells at one time and avoid cross-contamination of the solutions in each receiving well.

[0004] One of the objectives of the present invention is achieved by adopting the following technical solutions:

[0005] A combined sampling device includes a combined plate, the combined plate includes a sample plate and a receiving plate, the receiving plate is installed on top of the sample plate, the sample plate is provided with a sample receiving portion, the receiving plate is provided with a plurality of receiving wells and a sample injection channel, the sample injection channel is communicated with the sample receiving portion and the receiving wells, the combined sampling device further includes a pipetting structure, the pipetting structure is communicated with a plurality of the receiving wells, the pipetting structure makes a negative pressure in a plurality of the receiving wells, and the liquid sample in the sample receiving portion flows into each of the receiving wells along the sample injection channel.

[0006] Further, the number of the sample injection channels is the same as the number of the receiving wells, each of the sample injection channels extends from the bottom of the outer wall of the receiving well to near the top, and the sample injection channel is communicated with the inside of the receiving well near the top of the receiving well.

[0007] Further, the sample plate is further provided with a sampling groove, a liquid inlet, an overflow port and a waste liquid groove, the sampling groove is communicated with the sample receiving portion through the liquid inlet, and the sample receiving portion is communicated with the waste liquid groove through the overflow port.

[0008] Further, the bottom of the sampling groove is inclined, and the lowest point of the bottom of the sampling groove is connected to the bottom of the liquid inlet.

[0009] Further, the sample receiving portion is a plurality of sample holes, and the plurality of sample holes are communicated with each other through a main channel.

[0010] Further, the pipetting structure includes a gas valve assembly, a piston assembly, and a valve core. The gas valve assembly is sealingly installed on the combined plate. The gas valve assembly is provided with a piston chamber and a pressure-changing chamber communicating with the piston chamber. The piston assembly is installed on the gas valve assembly and extends into the piston chamber. The valve core includes a second slider provided with a sixth through hole. The valve core is installed in the pressure-changing chamber. The second slider abuts against the bottom wall of the pressure-changing chamber to disconnect the pressure-changing chamber from the receiving hole. The piston assembly moves in the piston chamber to change the pressure in the pressure-changing chamber. When the pressure in the pressure-changing chamber decreases, a pressure difference is formed at both ends of the second slider, and the second slider separates from the bottom wall of the pressure-changing chamber. The pressure-changing chamber communicates with the receiving hole through the sixth through hole, and a negative pressure is generated in the receiving hole. The liquid sample in the sample receiving portion flows into the receiving hole along the sample injection channel respectively.

[0011] Further, the valve core further includes a first slider. When the pressure in the pressure-changing chamber increases, a pressure difference is formed at both ends of the first slider, and the first slider drives the second slider to move so that the second slider separates from the bottom wall of the pressure-changing chamber. The pressure-changing chamber communicates with the receiving hole through the sixth through hole, and the compressed gas in the pressure-changing chamber is injected into the receiving hole, and the pipetting structure separates from the combined plate.

[0012] Further, the pipetting structure further includes an elastic member. Both ends of the elastic member abut against the valve core and the gas valve assembly respectively, and the elastic force of the elastic member makes the second slider abut against the bottom wall of the pressure-changing chamber.

[0013] Further, the gas valve assembly is further provided with a third through hole located between the pressure-changing chamber and the receiving hole. The cross-sectional area of the third through hole is smaller than the cross-sectional area of the bottom of the pressure-changing chamber. A table surface is formed between the third through hole and the bottom of the pressure-changing chamber. When the second slider abuts against the bottom wall of the pressure-changing chamber, the sixth through hole abuts against the table surface.

[0014] Further, the pipetting structure is a vacuum pump.

[0015] Compared with the prior art, the combined plate of the combined sample adding device of the present invention includes a sample plate and a receiving plate. The receiving plate is installed on the sample plate. The sample plate is provided with a sample receiving portion. The receiving plate is provided with a plurality of receiving holes and a sample injection channel. The sample injection channel communicates with the sample receiving portion and the receiving holes. The combined sample adding device further includes a pipetting structure. The pipetting structure communicates with the plurality of receiving holes. The pipetting structure makes the plurality of receiving holes form a negative pressure, and the liquid sample in the sample receiving portion flows into each receiving hole along the sample injection channel respectively. Through the above design, it is possible to add samples to a plurality of receiving holes at one time and avoid mutual mixing and contamination of the solutions in each receiving hole. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a perspective view of the modular sampling device of the present invention;

[0017] Figure 2 is Figure 1 an exploded view of the modular sampling device of;

[0018] Figure 3 is Figure 2 an exploded view of the combination plate of the modular sampling device of;

[0019] Figure 4 is Figure 3 a perspective view of the sample plate of the combination plate of;

[0020] Figure 5 is Figure 3 a perspective view of the accommodation plate of the combination plate of;

[0021] Figure 6 is Figure 3 another perspective view of the accommodation plate of the combination plate of;

[0022] Figure 7 is Figure 3 a three-dimensional sectional view of the combination plate of;

[0023] Figure 8 is Figure 7 an enlarged view of part A of the combination plate of;

[0024] Figure 9 is Figure 1 an exploded view of the pipetting structure of the modular sampling device of;

[0025] Figure 10 is Figure 9 a perspective view of the upper air valve seat of the pipetting structure of;

[0026] Figure 11 is Figure 9 a perspective view of the valve core of the pipetting structure of;

[0027] Figure 12 is Figure 1 a sectional view of the modular sampling device of;

[0028] Figure 13 is Figure 12 a schematic diagram of the local structure of the modular sampling device of.

[0029] In the figure: 10, combined plate; 11, sample plate; 111, sample addition groove; 112, liquid inlet; 113, main flow channel; 114, sample accommodation part; 115, overflow port; 116, waste liquid tank; 117, first sealing groove; 12, accommodation plate; 121, accommodation hole; 122, sample injection flow channel; 123, sample addition port; 124, first end face; 125, second end face; 126, second sealing groove; 13, first sealing ring; 20, pipetting structure; 21, air valve assembly; 210, sealing gasket; 2101, first through hole; 211, lower seat; 2110, first fixing hole; 2111, second through hole; 2112, third through hole; 212, gasket; 2120, fourth through hole; 2121, second fixing hole; 213, upper seat; 2130, mounting hole; 2132, fifth through hole; 2133, third fixing hole; 214, second sealing ring; 215, piston seat; 2150, piston cavity; 216, fixing member; 217, variable pressure cavity; 22, piston assembly; 220, end cover; 221, piston rod; 23, valve core; 231, slide bar; 232, first slider; 233, second slider; 2330, sixth through hole; 24, elastic member. Detailed implementation manner

[0030] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0031] It should be noted that when a component is referred to as being "fixed to" another component, it can be directly on the other component or there can also be another intermediate component through which it is fixed. When a component is considered to be "connected" to another component, it can be directly connected to the other component or there may be another intermediate component at the same time. When a component is considered to be "disposed on" another component, it can be directly disposed on the other component or there may be another intermediate component at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this invention belongs. The terms used herein in the description of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0033] Such as Figures 1 to 13As shown in the figure, the combined sampling device of the present invention includes a combined plate 10 and a pipetting structure 20.

[0034] The combined plate 10 includes a sample plate 11, a receiving plate 12, and a first sealing ring 13.

[0035] The sample plate 11 is provided with a sampling groove 111, a liquid inlet 112, a main flow channel 113, a sample receiving part 114, an overflow port 115, a waste liquid tank 116, and a first sealing groove 117. The sampling groove 111 is located at the end of the sample plate 11. In this embodiment, the sampling groove 111 is strip-shaped, and the bottom of the sampling groove 111 is an inclined surface. The lowest point of the inclined surface is connected to the liquid inlet 112, and the liquid inlet 112 is located between the sampling groove 111 and the sample receiving part 114. The solution sample enters the sample receiving part 114 through the sampling groove 111 and the liquid inlet 112. In this embodiment, the sample receiving part 114 is a plurality of cylindrical holes, and the plurality of cylindrical holes are interconnected through the main flow channel 113. This design enables the sample receiving part 114 to be filled with only a small amount of solution sample. In other embodiments, the sample receiving part 114 may also be a rectangular groove. The overflow port 115 is located at the other end of the sample receiving part 114. When too much solution sample is added, the solution sample overflows from the overflow port 115 to the waste liquid tank 116 to maintain the uniformity of the height of the sample solution. The first sealing groove 117 is used to install the first sealing ring 13, and the first sealing ring 13 seals the sample plate 11 and the receiving plate 12 to prevent the sample solution in the sample plate 11 from overflowing through the contact surface.

[0036] The receiving plate 12 is provided with a plurality of receiving holes 121, a plurality of sample injection channels 122, a sampling port 123, a first end face 124, a second end face 125, and a second sealing groove 126. The plurality of receiving holes 121 are provided on the first end face 124, and each receiving hole 121 extends downward from the first end face 124. The number of the sample injection channels 122 is the same as the number of the receiving holes 121. Each sample injection channel 122 extends from the bottom of the outer wall of the receiving hole 121 to a position near the top of the receiving hole 121. The sample injection channel 122 communicates with the inside of the receiving hole 121 near the top of the receiving hole 121. The sampling port 123 is located at the end of the receiving plate 12, and the sampling port 123 is used to pour the solution sample plate into the sampling groove 111. The height of the first end face 124 is lower than that of the second end face 125 to facilitate the contact and cooperation between the first end face 124 and the lower end face of the gasket 210 to achieve strict sealing. The second sealing groove 126 is used to install the first sealing ring 13, and the first sealing ring 13 seals the sample plate 11 and the receiving plate 12 to prevent the sample solution in the sample plate 11 from overflowing through the contact surface.

[0037] The receiving plate 12 is installed on the sample plate 11, the receiving holes 121 extend into the sample receiving part 114, the position of the sampling port 123 corresponds to that of the sampling groove 111, and the first sealing ring 13 seals the sample plate 11 and the receiving plate 12.

[0038] The pipetting structure 20 includes a gas valve assembly 21, a piston assembly 22, a valve core 23, and an elastic member 24.

[0039] The gas valve assembly 21 includes a gasket 210, a lower seat 211, a spacer 212, an upper seat 213, a plurality of second sealing rings 214, a piston seat 215, and a fixing member 216 arranged in sequence.

[0040] The gasket 210 is used to keep the pipetting structure 20 sealed with the combination plate 10 and prevent air leakage between the pipetting structure 20 and the combination plate 10. The gasket 210 is provided with a first through hole 2101, and the first through hole 2101 communicates with the accommodation hole 121 to adjust the air pressure in the accommodation hole 121.

[0041] The lower seat 211 is provided with a first fixing hole 2110, a second through hole 2111, and a third through hole 2112. The first fixing hole 2110 is used to install the fixing member 216. The second through hole 2111 is used to accommodate the second slider 233 of the valve core 23. The second through hole 2111 is cylindrical, and the third through hole 2112 is also cylindrical. The diameter of the second through hole 2111 is larger than the diameter of the third through hole 2112. The center lines of the second through hole 2111 and the third through hole 2112 are located on the same axis. A table surface is formed between the second through hole 2111 and the third through hole 2112.

[0042] The spacer 212 is provided with a fourth through hole 2120 and a second fixing hole 2121. The fourth through hole 2120 is used for gas flow and the slide bar 231 of the valve core 23 to extend into. The second fixing hole 2121 is used to install the fixing member 216.

[0043] The upper seat 213 is provided with a mounting hole 2130, a fifth through hole 2132, and a third fixing hole 2133. The mounting hole 2130 is used to install the valve core 23. The fifth through hole 2132 is used for gas flow. The third fixing hole 2133 is for the fixing member 216.

[0044] The number of the second sealing rings 214 is multiple, and the multiple second sealing rings 214 are used to seal the piston rod 221.

[0045] The piston seat 215 is provided with a piston cavity 2150.

[0046] The fixing member 216 is used to connect and fix the lower seat 211, the spacer 212, and the upper seat 213. Specifically, the fixing member 216 is a screw.

[0047] The fixing member 216 extends into the first fixing hole 2110 of the lower seat 211, the second fixing hole 2121 of the spacer 212, and the third fixing hole 2133 of the upper seat 213 to fix the lower seat 211, the spacer 212, and the upper seat 213 in sequence. At this time, the fifth through hole 2132, the fourth through hole 2120, and the second through hole 2111 together form a pressure-changing cavity 217.

[0048] The piston assembly 22 includes an end cap 220 and a plurality of piston rods 221. The piston assembly 22 is installed on the valve assembly 21, and the piston rods 221 extend into the piston chamber 2150.

[0049] The valve core 23 includes a sliding rod 231, a first slider 232, and a second slider 233. The first slider 232 and the second slider 233 extend from the sliding rod 231. The second slider 233 is located at the end of the valve core 23. The second slider 233 is provided with a sixth through hole 2330. The valve core 23 is installed in the pressure-changing chamber 217, and a part of the second slider 233 abuts against the bottom wall of the pressure-changing chamber 217 (the table surface of the second through hole 2111 and the third through hole 2112). At this time, the sixth through hole 2330 abuts against the bottom wall of the pressure-changing chamber 217, disconnecting the third through hole 2112 from the pressure-changing chamber 217.

[0050] The elastic member 24 is sleeved on the sliding rod 231 of the valve core 23, and both ends of the elastic member 24 abut against the upper wall of the mounting hole 2130 of the valve assembly 21 and the first slider 232 of the valve core 23 respectively. Specifically, the elastic member 24 is a spring.

[0051] When using the combined sampling device, the liquid transfer structure 20 is installed on the combined plate 10, and the gasket 210 contacts the first end face 124 to achieve sealing. The solution sample enters the sample receiving portion 114 through the sampling groove 111 and the liquid inlet 112.

[0052] By controlling the piston assembly 22 to move regularly up and down through an external robotic arm or other controller, when the piston rod 221 moves upward, the piston chamber 2150 sucks air into the pressure-changing chamber 217, resulting in a decrease in the gas pressure in the pressure-changing chamber 217. At this time, a pressure difference is formed between the upper and lower ends of the second slider 233. Under the action of the pressure, the elastic member 24 is further compressed, the valve core 23 moves upward, and the sixth through hole 2330 connects the pressure-changing chamber 217, the third through hole 2112, and the receiving hole 121. Under the negative pressure, the pressure-changing chamber 217 sucks the air in the receiving hole 121 through the third through hole 2112. Since the air in the receiving hole 121 decreases to form a negative pressure, a pressure difference is formed at both ends of the liquid level of the solution sample in the sample receiving portion 114. Under the action of the external atmospheric pressure, the sample solution is pressed into the receiving hole 121 through the sampling flow channel 122 to achieve mixing with the drug. The amount of the sample solution added into the receiving hole 121 is controlled by controlling the movement stroke of the piston rod 221.

[0053] After the movement of the piston rod 221 stops, the pressure difference at both ends of the second slider 233 decreases. Under the action of the elastic member 24, the valve core 23 moves downward, and the gas channel between the pressure-changing chamber 217 and the receiving hole 121 is closed.

[0054] When the piston rod 221 moves downward, the piston chamber 2150 injects air into the pressure-changing chamber 217, resulting in an increase in the gas pressure in the pressure-changing chamber 217. A pressure difference is formed between the upper and lower ends of the first slider 232. Under the action of the pressure, the elastic member 24 is further compressed, and the valve core 23 moves upward. The sixth through hole 2330 connects the pressure-changing chamber 217 and the receiving hole 121. Under the action of the air pressure, the compressed gas in the pressure-changing chamber 217 is injected into the receiving hole 121. Under the action of the air pressure, the combined plate 10 receives a downward acting force exerted by the liquid transfer structure 20, and the combined plate 10 is separated from the liquid transfer structure 20, completing the entire liquid transfer process.

[0055] In other embodiments, the liquid transfer structure 20 is a vacuum pump, and the vacuum pump is used to evacuate the receiving hole 121, thereby completing the liquid transfer operation from the sample receiving portion 114 to the receiving hole 121.

[0056] In this application, the combined plate 10 and the liquid transfer structure 20 cooperate to accurately transfer the sample solution from the sample plate 11 to the receiving plate 12, eliminating the cumbersome process of individually transferring liquid in a single hole of the traditional combined plate, and at the same time avoiding the waste caused by replacing disposable pipette tips.

[0057] The above embodiments only represent several implementation manners of the present invention, and the description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several deformations and improvements can be made. These are all equivalent modifications and evolutions of the above embodiments based on the essence of the present invention, and all belong to the protection scope of the present invention.

Claims

1. A combined sample adding device, comprising a combined plate, characterized in that: The combined plate includes a sample plate and a receiving plate. The receiving plate is installed on top of the sample plate. The sample plate is provided with a sample receiving portion, and the sample receiving portion is a plurality of sample holes. The plurality of sample holes are communicated through a main flow channel. The receiving plate is provided with a plurality of receiving holes and a sample injection flow channel. The number of the sample injection flow channels is the same as the number of the receiving holes. Each sample injection flow channel extends from the bottom of the outer wall of the receiving hole to near the top, and the sample injection flow channel communicates with the inside of the receiving hole at the position near the top of the receiving hole. The sample injection flow channel communicates with the sample receiving portion and the receiving hole. The combined sample injection device further includes a liquid transfer structure, and the liquid transfer structure communicates with the plurality of receiving holes. The liquid transfer structure makes the plurality of receiving holes form negative pressure, and the liquid samples in the sample receiving portion flow into each receiving hole along the sample injection flow channel respectively. The liquid transfer structure includes a gas valve assembly, a piston assembly and a valve core. The gas valve assembly is sealed and installed on the combined plate. The gas valve assembly is provided with a piston chamber and a pressure-changing chamber communicated with the piston chamber. The piston assembly is installed on the gas valve assembly and extends into the piston chamber. The valve core includes a second slider, and the second slider is provided with a sixth through hole. The valve core is installed in the pressure-changing chamber. The second slider abuts against the bottom wall of the pressure-changing chamber to disconnect the pressure-changing chamber from the receiving hole. The piston assembly moves in the piston chamber to change the pressure in the pressure-changing chamber. When the pressure in the pressure-changing chamber decreases, a pressure difference is formed at both ends of the second slider, and the second slider separates from the bottom wall of the pressure-changing chamber. The pressure-changing chamber communicates with the receiving hole through the sixth through hole, and negative pressure is generated in the receiving hole. The liquid samples in the sample receiving portion flow into the receiving holes along the sample injection flow channel respectively.

2. The combined sample adding device according to claim 1, characterized in that: The sample plate is further provided with a sample injection groove, a liquid inlet, an overflow port and a waste liquid groove. The sample injection groove communicates with the sample receiving portion through the liquid inlet. The sample receiving portion communicates with the waste liquid groove through the overflow port.

3. The modular sampling device according to claim 2, characterized in that: The bottom of the sample injection groove is inclined, and the lowest point of the bottom of the sample injection groove is connected to the bottom of the liquid inlet.

4. The combined sample adding device according to claim 1, characterized in that: The valve core further includes a first slider. When the pressure in the pressure-changing chamber increases, a pressure difference is formed at both ends of the first slider, and the first slider drives the second slider to move so that the second slider separates from the bottom wall of the pressure-changing chamber. The pressure-changing chamber communicates with the receiving hole through the sixth through hole, and the compressed gas in the pressure-changing chamber is injected into the receiving hole, and the liquid transfer structure is separated from the combined plate.

5. The modular sampling device according to claim 4, wherein: The liquid transfer structure further includes an elastic member, and both ends of the elastic member abut against the valve core and the gas valve assembly respectively. The elastic force of the elastic member makes the second slider abut against the bottom wall of the pressure-changing chamber.

6. The combined sampling device according to claim 1, wherein: The gas valve assembly is further provided with a third through hole, and the third through hole is located between the pressure-changing chamber and the receiving hole. The cross-sectional area of the third through hole is smaller than the cross-sectional area of the bottom of the pressure-changing chamber. A table surface is formed between the third through hole and the bottom of the pressure-changing chamber. When the second slider abuts against the bottom wall of the pressure-changing chamber, the sixth through hole abuts against the table surface.

7. The combined sampling device according to any one of claims 1-3, characterized in that: The liquid transfer structure is a vacuum pump.

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