A kind of rose residue biological organic fertilizer and its preparation method and application

Abstract

The present invention relates to a rose dregs bio-organic fertilizer and a preparation method and application thereof, and belongs to the technical field of microbial fertilizers. Rose dregs contain a variety of nutrients such as rich sugars and amino acids. EM bacterial agents and Halobacillus salinus CGMCC NO.1.8606 are used as fermentation agents, and rose dregs are used as fermentation substrates to produce bio-organic fertilizers. This can improve the utilization rate of rose dregs, and the prepared bio-organic fertilizers can replace inorganic fertilizers, reduce the use of inorganic fertilizers in crop production, and alleviate various problems caused by excessive use of inorganic fertilizers. The prepared bio-organic fertilizers can effectively increase crop yields, promote crop early maturity, significantly reduce crop maturity shipping time, extend crop harvesting period, improve crop quality, and produce a variety of substances required by plants.

Description

Technical Field

[0001] The invention relates to a rose residue biological organic fertilizer and a preparation method and application thereof, belonging to the technical field of marine microorganism application and microbial fertilizer. Background Art

[0002] Rose (Rosa rugosa) is a perennial evergreen or deciduous shrub of the genus Rosa in the Rosaceae family, and is widely planted around the world. my country is one of the major rose-growing countries with rich rose resources, with a total of 95 species of more than 200 species of the genus Rosa. The main rose varieties grown in my country are Floribunda Rose and Kushui Rose, which are mainly distributed in Pingyin, Shandong, Yongdeng, Gansu, etc., with a total cultivation area of ​​7,000 hm2. 2 About. Rose contains essential oil and pigment, and the essential oil and pigment extracted from rose has many advantages. Rose essential oil has an elegant, soft, delicate, sweet and fragrant rose fragrance, and is known as "liquid gold". The extraction of rose essential oil generally adopts the process of steam distillation, and the essential oil extraction rate is about 0.25-0.3‰, which will produce a large amount of rose slag. Rose pigment is easily soluble in water and is an excellent natural pigment. Its use in food effectively makes up for the shortcomings of chemical synthetic pigments. After the extraction of rose pigment, a large amount of rose slag will also be produced. In addition, rose is a natural raw material with very rich nutrients, which contains a variety of trace elements, dietary fiber and essential amino acids for the human body. In recent years, roses have also been used in brewing technology to extract the active ingredients in roses, or directly add fresh or dried roses to the beer brewing process to give beer the flavor or efficacy of roses. This process also produces a large amount of rose slag.

[0003] In actual production, rose dregs are mostly thrown away or burned as waste, which not only pollutes the environment, but also wastes rose dregs resources. Wei Qin et al. analyzed the nutritional components contained in rose dregs, and the results showed that the sugar content was 29.5%, of which soluble sugar was 8.8%; the total amino acid content was 10.205%, including 8 essential amino acids for the human body; it also contained VC, VE, β-carotene and Ca, Fe, Mn and other mineral elements. (Wei Qin et al. Study on the Chemical Composition and Nutritional Components of Rose Dregs [J]. Journal of Northwest Forestry University, 2005, 20 (3): 140-141.) Fan Zhujun et al. analyzed the components of rose dregs after essential oil was extracted by steam distillation, among which the glucose content was 18.33%, the starch content was 21.75%, the crude fat content was 3.09%, the crude protein content was 12.40%, and the crude fiber content was 21.28%. (Fan Zhujun et al. Preliminary Study on the Components of Rose Pomace [J]. Natural Product Research and Development, 1990 (1): 67-69.) Xie Qiutao determined that the total relative content of dietary fiber in rose pomace after oil and pigment extraction was 35.9%, of which the relative content of insoluble fiber was 32.9% and the relative content of soluble fiber was 3.0%. (Xie Qiutao. Study on Optimization of Supercritical CO2 Extraction of Rose Essential Oil and Comprehensive Utilization of Byproducts [D]. Changsha: Central South University, 2013.) From the analysis results of trace elements, nutrients, amino acid content, etc. of rose pomace, it can be seen that rose pomace is a natural raw material with rich nutrients and is worthy of further development and utilization.

[0004] Chinese patent document CN104351839A (application number 201410618093.4) ​​provides a method for preparing salted eggs using rose slag, comprising the following steps: heating and boiling the rose slag, controlling the salt content to be 15% to 18% to obtain rose slag liquid; cooling the rose slag liquid, then adding sodium dehydroacetate and potassium sorbate, mixing, and obtaining rose slag pickling liquid; immersing fresh eggs in the rose slag pickling liquid, sealing, and pickling at 20°C to 28°C for 25 to 30 days to complete the preparation of salted eggs. The present invention uses rose slag to prepare salted eggs, which has the advantages of simple preparation, unique salted egg flavor, good taste, and high nutritional value.

[0005] Chinese patent document CN106420537A (application number 201611059183.X) discloses a rose slag exfoliating massage bath cream, which is mainly composed of rose slag, highland barley flour, oatmeal flour, rose petals, rose essential oil, olive oil, vaseline, and nonionic surfactant. Most of the existing commercially available exfoliating products are for facial skin, which contain chemical substances, are highly irritating to the skin, and long-term use can cause skin problems. The rose slag exfoliating massage bath cream of the present invention is composed of pure natural plant ingredients, and rose slag is used as the main component of the exfoliating massage bath cream to avoid waste of resources. No irritation and side effects will be produced during use, and it can be used for a long time, and is suitable for whole body exfoliation. The rose slag exfoliating massage bath cream can gently remove the keratin accumulation caused by metabolism, dryness and water shortage in the body, soothe the skin, effectively repair dry skin, and make the skin lastingly moisturized.

[0006] Chinese patent document CN108277113A (application number 201810252210.8) discloses a rose slag soap, which is mainly composed of edible rose slag, edible rose petals, olive oil, coconut oil, palm oil, sodium hydroxide, distilled water, citric acid, edible ethanol, sodium chloride, and sodium carbonate. The rose slag soap of the present invention does not contain substances harmful to the human body, and during use, it will not have side effects on the human body and can be used for a long time; and the edible rose slag is used as the main component of the soap, which improves the utilization rate of the rose slag and avoids waste of resources and environmental pollution. The rose slag soap has an elegant and lasting, pleasant rose fragrance, can kill bacteria and resist bacteria, clean the skin, prevent skin aging, make people feel comfortable and happy, relieve anxiety and depression, adjust female endocrine and menstrual cycle, and also has a good effect of removing athlete's foot and foot odor.

[0007] In addition, the prior art also discloses the extraction of polyphenols (such as CN109529404A), flavonoids (such as CN106317005A), lignin (such as CN105559095A), dietary fiber (such as CN105249482A), amino acids (such as CN108186429A), polysaccharides (such as CN105669624A), etc. from rose residues, thereby further improving the utilization rate of rose residues.

[0008] Developing purely natural organic fertilizers to replace organic chemical fertilizers will greatly help improve this situation. Summary of the invention

[0009] In view of the deficiencies of the prior art, the present invention provides a rose dregs bio-organic fertilizer and a preparation method and application thereof, wherein the rose dregs are used as a fermentation substrate and the bio-organic fertilizer is obtained after fermentation with a fermentation agent. The rose dregs organic fertilizer of the present invention has the functions of improving soil, fixing nitrogen, dissolving phosphorus and potassium, and while replacing inorganic fertilizers, it can also improve the quality of crops, promote early natural maturity of crops and extend the harvest period.

[0010] The technical solution of the present invention is as follows:

[0011] A method for preparing rose slag bio-organic fertilizer, comprising the following steps:

[0012] (1) Preparation of rose residue fermentation matrix: taking rose residue, adding water to adjust the water content of the rose residue to 70-80%, using brown sugar to adjust the total sugar content of the rose residue to 30-35%, and sterilizing at high temperature to obtain the rose residue fermentation matrix;

[0013] (2) Activation of the fermentation agent: The fermentation agent includes EM agent and Halobacillus salinus CGMCC No. 1.8606. The EM agent and Halobacillus salinus CGMCC No. 1.8606 are activated respectively using 0.2% brown sugar water, and the activation culture is carried out at 35-37° C. for 1-2 days. The activated EM agent and Halobacillus salinus CGMCC No. 1.8606 are mixed to obtain a fermentation agent.

[0014] (3) inoculating the fermentation agent prepared in step (2) into the rose residue fermentation matrix prepared in step (1), sealing the tank, and fermenting at 30-40° C. for 7-10 days to obtain a fermentation liquid; separating the fermentation liquid from solid and liquid, wherein the liquid is the rose residue biological organic liquid fertilizer, and the solid is dried and crushed to obtain the rose residue biological organic solid fertilizer.

[0015] Preferably, according to the present invention, water is added in step (1) to adjust the moisture content of the rose residue to 70%.

[0016] Preferably, in step (1), brown sugar is used to adjust the total sugar content of the rose residue to 30%.

[0017] Preferably, in step (2), the EM bacterial agent and the Halobacillus salinus CGMCC NO.1.8606 are both bacterial powders, the amount of the EM bacterial agent is 0.04-0.10% of the mass of the rose residue, and the amount of the Halobacillus salinus CGMCC NO.1.8606 is 0.05-0.10% of the mass of the rose residue.

[0018] Further preferably, the dosage of the EM bacterial agent is 0.05% of the mass of the rose residue, and the dosage of the Halobacillus salinus CGMCC NO.1.8606 is 0.07% of the mass of the rose residue.

[0019] Preferably according to the present invention, plate filter press is used for solid-liquid separation in step (3).

[0020] Preferably according to the present invention, the drying temperature in step (3) is 60-65°C.

[0021] The rose residue biological organic liquid fertilizer is prepared according to the above method.

[0022] The rose residue biological organic solid fertilizer prepared according to the method is obtained.

[0023] The application of the rose residue bio-organic fertilizer in crop cultivation and production.

[0024] The operation steps not described in detail in the present invention are all routine operations in the art.

[0025] In the present invention, the sugar content of rose dregs is adjusted to provide sufficient nutrients for the fermentation agent, improve the fermentation activity, and produce more beneficial active ingredients; brown sugar is used to adjust the sugar content of rose dregs, mainly because the price of brown sugar is low, which can reduce the production cost of rose dregs biological organic fertilizer, but other ingredients such as molasses can also be used to adjust the sugar content of rose dregs. The present invention also uses 0.2% brown sugar water to activate the fermentation agent, which simplifies the operation steps of strain activation on the basis of controlling the production cost. If a conventional strain culture medium such as LB culture medium is used, the purpose of strain activation can also be achieved, but the cost is high and the operation is complicated.

[0026] Beneficial effects:

[0027] 1. Rose residue is rich in sugar, amino acids and other nutrients. Using rose residue as a fermentation matrix to ferment and produce biological organic fertilizer can not only effectively solve the recycling of rose residue and further improve the utilization rate of rose residue, but the prepared biological organic fertilizer can also replace inorganic fertilizers, reduce the use of inorganic fertilizers in crop production, and alleviate various problems caused by excessive use of inorganic fertilizers.

[0028] 2. The present invention further optimizes the fermentation strain of the rose residue biological organic fertilizer, that is, Halobacillus salinus CGMCC NO.1.8606 is added to the EM bacterial agent, and the EM bacterial agent and the Halobacillus salinus CGMCC NO.1.8606 interact with each other. The biological organic fertilizer prepared by the mixed bacterial agent can effectively increase the yield of crops, promote the early maturity of crops, significantly reduce the shipping time of mature crops, extend the harvest period of crops, improve the quality of crops, produce various substances required by plants, and also has the function of improving soil, and plays the role of nitrogen fixation, potassium dissolution and phosphorus dissolution.

[0029] 3. The rose residue bio-organic fertilizer prepared by the present invention includes rose residue bio-organic liquid fertilizer and rose residue bio-organic solid fertilizer. Those skilled in the art can reasonably choose to apply different types of rose residue bio-organic fertilizers according to the types and growth stages of crops. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a picture of cucumbers harvested in Experimental Example 1.

[0031] Figure 2 This is a picture of the chrysanthemum harvested in Experimental Example 2.

[0032] Figure 3 This is a picture of ginger harvested in Experimental Example 3.

[0033] Figure 4 This is a picture of the strawberry plants cultivated in Experimental Example 4.

[0034] Figure 5 This is a picture of corn plants planted in Experimental Example 5.

[0035] Figure 6 This is a picture of corn harvested in Experimental Example 5.

[0036] Figure 7 This is a picture of apples harvested in Experimental Example 6.

[0037] Figure 8 This is a picture of the Chinese cabbage seedlings cultivated in Experimental Example 7. DETAILED DESCRIPTION

[0038] The technical scheme of the present invention is further described below in conjunction with the embodiments, but the protection scope of the present invention is not limited thereto. The raw materials and medicines involved in the embodiments, unless otherwise specified, are conventional commercial products in the art. The experimental operations involved in the embodiments, unless otherwise specified, are conventional operations in the art. The percentages in the embodiments, unless otherwise specified, are all mass percentages.

[0039] EM bacterial agent is a conventional commercially available bacterial agent and can be purchased from Jining Jinshan Bioengineering Co., Ltd.

[0040] The rose residue of the present invention is purchased from Shandong Huamei Biotechnology Co., Ltd. and is a waste material obtained after a high-temperature cooking process.

[0041] The main nutrient content of the above rose dregs is as follows:

[0042] Table 1. Main nutritional content of rose dregs

[0043] Nutritional Information content Total sugar / % 25.5 Reducing sugar / % 8.3 Soluble sugar / % 9.0 Total acid (calculated as malic acid) / % 2.05 Ash / % 3.26

[0044] The halophilic Bacillus involved in the examples are Halobacillus salinus CGMCCNO.1.8606 and Halobacillus salinus CGMCCNO.1.15964, both of which were purchased from China General Microbiological Culture Collection Center.

[0045] Among them, Halobacillus salinus CGMCC NO.1.8606 was isolated from the sediments of the Pacific Polymetallic Contract Area. It is a marine microorganism with certain halophilic characteristics.

[0046] Embodiment 1:

[0047] A method for preparing rose slag bio-organic fertilizer, comprising the following steps:

[0048] (1) Preparation of rose residue fermentation matrix: taking rose residue, adding water to adjust the water content of the rose residue to 70%, using brown sugar to adjust the total sugar content of the rose residue to 30%, and sterilizing at high temperature to obtain the rose residue fermentation matrix;

[0049] (2) Activation of the fermentation agent: The fermentation agent includes EM agent and Halobacillus salinus CGMCC NO.1.8606, both of which are bacterial powders, and the amount of EM agent is 0.05% of the mass of rose dregs, and the amount of Halobacillus salinus CGMCC NO.1.8606 is 0.07% of the mass of rose dregs; EM agent and Halobacillus salinus CGMCC NO.1.8606 are activated by using 0.2% brown sugar water, and the activated culture is carried out at 35° C. for 1 day, and the activated EM agent and Halobacillus salinus CGMCC NO.1.8606 are mixed to obtain a fermentation agent;

[0050] (3) placing the rose residue fermentation matrix prepared in step (1) in a fermentation tank, adding the fermentation agent prepared in step (2), sealing the tank, and fermenting at 37° C. for 7 days to obtain a fermentation liquid; using a plate filter press to separate the fermentation liquid into solid and liquid, the liquid is the rose residue biological organic liquid fertilizer, and the solid is dried at 60° C. and crushed to obtain the rose residue biological organic solid fertilizer.

[0051] Embodiment 2:

[0052] A method for preparing rose slag bio-organic fertilizer, comprising the following steps:

[0053] (1) Preparation of rose residue fermentation matrix: taking rose residue, adding water to adjust the water content of the rose residue to 75%, using brown sugar to adjust the total sugar content of the rose residue to 32%, and sterilizing at high temperature to obtain the rose residue fermentation matrix;

[0054] (2) Activation of the fermentation agent: The fermentation agent includes EM agent and Halobacillus salinus CGMCC NO.1.8606, both of which are bacterial powders, and the amount of EM agent is 0.06% of the mass of rose dregs, and the amount of Halobacillus salinus CGMCC NO.1.8606 is 0.08% of the mass of rose dregs; EM agent and Halobacillus salinus CGMCC NO.1.8606 are activated by using 0.2% brown sugar water, and the activated culture is carried out at 36° C. for 1 day, and the activated EM agent and Halobacillus salinus CGMCC NO.1.8606 are mixed to obtain a fermentation agent;

[0055] (3) placing the rose residue fermentation matrix prepared in step (1) in a fermentation tank, adding the fermentation agent prepared in step (2), sealing the tank, and fermenting at 35° C. for 8 days to obtain a fermentation liquid; using a plate filter press to separate the fermentation liquid into solid and liquid, the liquid is the rose residue biological organic liquid fertilizer, and the solid is dried at 60° C. and crushed to obtain the rose residue biological organic solid fertilizer.

[0056] Embodiment 3:

[0057] A method for preparing rose slag bio-organic fertilizer, comprising the following steps:

[0058] (1) Preparation of rose residue fermentation matrix: taking rose residue, adding water to adjust the water content of the rose residue to 80%, using brown sugar to adjust the total sugar content of the rose residue to 35%, and sterilizing at high temperature to obtain the rose residue fermentation matrix;

[0059] (2) Activation of the fermentation agent: The fermentation agent includes EM agent and Halobacillus salinus CGMCC NO.1.8606, both of which are bacterial powders, and the amount of EM agent is 0.07% of the mass of rose dregs, and the amount of Halobacillus salinus CGMCC NO.1.8606 is 0.10% of the mass of rose dregs; EM agent and Halobacillus salinus CGMCC NO.1.8606 are activated by using 0.2% brown sugar water, and the activated culture is carried out at 37° C. for 1 day, and the activated EM agent and Halobacillus salinus CGMCC NO.1.8606 are mixed to obtain a fermentation agent;

[0060] (3) placing the rose residue fermentation matrix prepared in step (1) in a fermentation tank, adding the fermentation agent prepared in step (2), sealing the tank, and fermenting at 30° C. for 10 days to obtain a fermentation liquid; using a plate filter press to separate the fermentation liquid into solid and liquid, the liquid is the rose residue biological organic liquid fertilizer, and the solid is dried at 65° C. and crushed to obtain the rose residue biological organic solid fertilizer.

[0061] Comparative Example 1:

[0062] A rose residue bio-organic fertilizer, the preparation method of which is the same as that of Example 1, except that: the fermentation agent is EM agent and Halobacillus salinus CGMCC NO.1.15964.

[0063] The rose residue bio-organic fertilizer prepared as above was applied to the cultivation or production of the following crops to verify the application effect of the rose residue bio-organic fertilizer.

[0064] Experimental Example 1: Cucumber Production

[0065] The rose residue bio-organic fertilizer prepared in Example 1 and commercially available urea were applied to cucumber production to verify the effect of the rose residue bio-organic fertilizer. The planting soil was a saline-alkali soil type.

[0066] The experimental group was applied with the rose dregs bio-organic solid fertilizer prepared in Example 1, the application amount of the rose dregs bio-organic solid fertilizer was 6 kg / mu, and the fertilization method was to sow once three days after the cucumbers were planted, and then sow again after an interval of 15 days. The control group was applied with commercially available urea, and the application amount and application method were the same as those of the experimental group.

[0067] The cucumbers grown are Figure 1 As shown, Figure A shows the experimental group using the rose residue bio-organic solid fertilizer of Example 1, and Figure B shows the control group using commercially available urea. It can be seen from the figure that the cucumber fruits in the experimental group are more emerald green and full, and the sales level has increased. Compared with the control group, the cucumber yield in the experimental group increased by more than 30%, the mature cucumber shipping time was reduced by more than 7 days, and the nutrients and microbial community structure in the soil of the experimental group were improved. The bio-organic fertilizer has the functions of nitrogen fixation, potassium solubilization, and phosphorus solubilization.

[0068] Experimental Example 2: Chrysanthemum chrysanthemum production

[0069] The rose residue bio-organic fertilizer prepared in Example 1 and commercially available urea were applied to Chrysanthemum chrysanthemum production to verify the effect of the rose residue bio-organic fertilizer. The planting soil was a slightly saline-alkali soil type.

[0070] The experimental group was treated with the rose residue bio-organic solid liquid fertilizer prepared in Example 1, the application amount of the rose residue bio-organic liquid fertilizer was 10 kg / mu, and the fertilization method was spraying the leaves once three days after the planting of Chrysanthemum chrysanthemum, and spraying again after an interval of 15 days. The control group was treated with commercially available urea, and the application amount and application method were the same as those of the experimental group.

[0071] The cultivated chrysanthemum Figure 2 As shown, the chrysanthemums on the left are the experimental group that applied the rose residue biological organic liquid fertilizer of Example 1, and the chrysanthemums on the right are the control group that applied commercially available urea. It can be seen from the figure that the chrysanthemums in the experimental group are fuller and the sales level has increased, while the chrysanthemums in the control group have hollow stems and poor quality. Compared with the control group, the yield of chrysanthemums in the experimental group increased by more than 30%, the mature shipping time of chrysanthemums was reduced by more than 5 days, and the nutrients and microbial community structure in the soil of the experimental group were improved. The biological organic fertilizer has the functions of nitrogen fixation, potassium solubilization, and phosphorus solubilization.

[0072] Experimental Example 3: Ginger Production

[0073] The rose residue bio-organic fertilizer prepared in Example 1 and Comparative Example 1 was applied to ginger production to verify the effect of the rose residue bio-organic fertilizer. The planting area was the Laiwu ginger producing area.

[0074] The experimental group applied the rose dregs biological organic solid fertilizer prepared in Example 1, the application amount of the rose dregs biological organic solid fertilizer was 8 kg / mu, and the fertilization method was to sow once three days after ginger planting and then sow again after an interval of 15 days. The control group applied the rose dregs biological organic solid fertilizer prepared in Comparative Example 1, and the application amount and application method were the same as those of the experimental group.

[0075] Ginger grown Figure 3As shown, the ginger on the left is the experimental group that applied the rose dregs biological organic solid fertilizer of Example 1, and the ginger on the right is the control group that applied the rose dregs biological organic solid fertilizer of Comparative Example 1. It can be seen from the figure that the ginger in the experimental group has fewer ginger tendons, better quality, and higher sales level. Compared with the control group, the ginger yield of the experimental group increased by more than 15%, the sweetness increased, and the mature ginger shipping time was reduced by more than 3 days.

[0076] Experimental Example 4: Strawberry Production

[0077] The rose residue bio-organic fertilizer prepared in Example 1 and Comparative Example 1 was applied to strawberry production to verify the effect of the rose residue bio-organic fertilizer.

[0078] The experimental group was applied with the rose residue bio-organic liquid fertilizer prepared in Example 1, the sowing amount of the rose residue bio-organic liquid fertilizer was 10 kg / mu, and the fertilization method was spraying the leaves once three days after the strawberry seedlings were planted, and spraying again after an interval of 15 days. The control group was applied with the rose residue bio-organic liquid fertilizer prepared in Comparative Example 1, and the application amount and application method were the same as those of the experimental group.

[0079] Cultivating viable strawberry seedlings Figure 4 As shown, the left picture shows the control group using the rose slag biological organic liquid fertilizer of comparative example 1, and the right picture shows the experimental group using the rose slag biological organic liquid fertilizer of embodiment 1. It can be seen from the figure that the root system of the strawberry seedlings cultivated by the experimental group is more developed, the seedling stems are short and strong, the quality is superior, and the survival rate of the seedlings reaches more than 90%. Compared with the control group, the strawberry yield of the experimental group increased by more than 20%, the fragrance was rich, the anthocyanin content was significantly improved, and the shipping time of the mature strawberries was reduced by more than 5 days.

[0080] Experimental Example 5: Corn Production

[0081] The rose residue bio-organic fertilizer prepared in Example 1 and commercially available urea were applied to corn planting to verify the effect of the rose residue bio-organic fertilizer.

[0082] The experimental group was treated with the rose dregs bio-organic solid fertilizer prepared in Example 1, the application amount of the rose dregs bio-organic solid fertilizer was 10 kg / mu, and the fertilization method was to apply it as a base fertilizer during corn planting. The control group was treated with commercially available urea, and the application amount and application method were the same as those of the experimental group.

[0083] Corn grown as Figure 5As shown, the left picture shows the experimental group that applied the rose residue biological organic solid fertilizer in Example 1, and the right picture shows the control group that applied urea. It can be seen from the figure that the leaves of the corn plants in the experimental group grew in an orderly manner and had a good ventilation effect, while the leaves of the corn plants in the control group grew in a disorderly manner and had a poor ventilation effect. The ventilation effect will affect pollination and fruit growth. When the corn matures, 50 corns are randomly picked, as shown in the figure. Figure 6 As shown, the corn on the top is the control group, and the corn on the bottom is the experimental group. Compared with the control group, the corn in the experimental group is larger, the corn kernels are fuller, and the yield is significantly increased.

[0084] Experimental Example 6: Apple Production

[0085] The rose residue bio-organic fertilizer prepared in Example 1 and Comparative Example 1 was applied to apple production to verify the effect of the rose residue bio-organic fertilizer.

[0086] The experimental group was applied with the rose dregs bio-organic solid fertilizer prepared in Example 1, the application amount of the rose dregs bio-organic solid fertilizer was 10 kg / mu, and it was applied during the bud stage. The control group was applied with the rose dregs bio-organic solid fertilizer prepared in Comparative Example 1, and the application amount and application method were the same as those of the experimental group.

[0087] The harvested apples Figure 7 As shown, the apples on the left are the experimental group that applied the rose residue biological organic solid fertilizer of Example 1, and the apples on the right are the control group that applied the rose residue biological organic solid fertilizer prepared in Comparative Example 1. It can be seen from the figure that the apple seeds in the control group are poorly developed, the core is cracked and easily infected by pathogens, and the flesh is loose and soft; while the apples in the experimental group have a dense tissue structure and the fruit mass of the same volume is relatively large. Compared with the control group, the apples harvested in the experimental group have a higher content of superoxide dismutase and other nutrients, a better taste, crisp and palatable, high sugar content and rich aroma, higher content of carotene and anthocyanin, delicate appearance and good coloring.

[0088] Experimental Example 7: Cultivation of Chinese Cabbage

[0089] The rose residue bio-organic fertilizer prepared in Example 1 and Comparative Example 1 was applied to the cultivation of pakchoi seedlings to verify the effect of the rose residue bio-organic fertilizer.

[0090] Chinese cabbage was planted in flower pots, 50 Chinese cabbage seeds were sown in each pot, and fertilizer was applied when the seeds were sown. The experimental group applied the rose residue biological organic solid fertilizer prepared in Example 1, and the application amount was 100 g / pot, the control group 1 applied the rose residue biological organic solid fertilizer prepared in Comparative Example 1, and the control group 2 applied commercially available urea, and the application amounts of the control groups 1 and 2 were the same as those of the experimental group.

[0091] The emergence of Chinese cabbage seedlings Figure 8As shown, the Chinese cabbage on the left is the control group 2 where urea is applied, the Chinese cabbage in the middle is the control group 1 where the rose residue biological organic solid fertilizer of comparative example 1 is applied, and the Chinese cabbage on the right is the experimental group where the rose residue biological organic solid fertilizer of embodiment 1 is applied. As can be seen from the figure, the Chinese cabbage seedlings of the experimental group grow more vigorously, the germination rate of the Chinese cabbage seedlings in the experimental group is 80-90%, the leaves are thick, and the root system is well-developed. The germination rate of the Chinese cabbage seedlings in the control group 1 is 50%, and the germination rate of the Chinese cabbage seedlings in the control group 2 is 60%.

[0092] It can be seen from the above experimental examples that the rose residue bio-organic fertilizer prepared by the present invention can effectively replace inorganic fertilizers, and can effectively increase crop yields, promote early maturity of crops, significantly reduce the shipping time of mature crops, extend the crop harvest period, and improve crop quality.

Claims

1. A method for preparing rose slag bio-organic fertilizer, characterized in that: Here are the steps: (1) Preparation of rose residue fermentation matrix: taking rose residue, adding water to adjust the water content of the rose residue to 70-80%, using brown sugar to adjust the total sugar content of the rose residue to 30-35%, and sterilizing at high temperature to obtain the rose residue fermentation matrix; (2) Activation of fermentation agents: The fermentation agents include EM agents and Halobacillus salinus CGMCC NO.1.8606, wherein the EM agents and Halobacillus salinus CGMCC NO.1.8606 are both bacterial powders, the amount of the EM agents is 0.04-0.10% of the mass of the rose dregs, and the amount of the Halobacillus salinus CGMCC NO.1.8606 is 0.05-0.10% of the mass of the rose dregs. The EM agents and Halobacillus salinus CGMCC NO.1.8606 are activated by using 0.2% brown sugar water, and the activation culture is carried out at 35-37° C. for 1-2 days. The activated EM agents and Halobacillus salinus CGMCC NO.1.8606 are then cultured for 1-2 days. NO.1.8606 mixed to obtain fermentation agent; (3) inoculating the fermentation agent prepared in step (2) into the rose residue fermentation matrix prepared in step (1), sealing the tank, and fermenting at 30-40° C. for 7-10 days to obtain a fermentation liquid; separating the fermentation liquid from solid and liquid, wherein the liquid is the rose residue biological organic liquid fertilizer, and the solid is dried and crushed to obtain the rose residue biological organic solid fertilizer.

2. The preparation method according to claim 1, characterized in that In step (1), water is added to adjust the water content of the rose residue to 70%.

3. The preparation method according to claim 1, characterized in that: In step (1), brown sugar is used to adjust the total sugar content of the rose residue to 30%.

4. The preparation method according to claim 1, characterized in that: The dosage of the EM bacterial agent is 0.05% of the mass of the rose dregs, and the dosage of the Halobacillus salinus CGMCC NO.1.8606 is 0.07% of the mass of the rose dregs.

5. The preparation method according to claim 1, characterized in that: In step (3), plate filter press is used for solid-liquid separation.

6. The preparation method according to claim 1, characterized in that: The drying temperature in step (3) is 60-65°C.

7. The rose residue biological organic liquid fertilizer prepared according to the preparation method of claim 1.

8. The rose residue biological organic solid fertilizer prepared according to the preparation method of claim 1.

9. Use of the rose residue bio-organic liquid fertilizer according to claim 7 or the rose residue bio-organic solid fertilizer according to claim 8 in crop cultivation and production.

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