Sterilization method of raw pseudo-ginseng powder

Abstract

The present application relates to the technical field of sterilization methods of raw panax notoginseng, and particularly relates to a sterilization method of raw panax notoginseng powder, which can quickly, efficiently and simply sterilize the raw panax notoginseng powder, and has been verified to have good killing effect on gram-negative bacteria such as pseudomonas aeruginosa, escherichia coli and staphylococcus aureus, and fungi such as candida albicans. More importantly, compared with the irradiation sterilization equipment, the equipment adopted by the present application is simple, easy to operate, and low in sterilization cost, and can effectively kill the microorganisms in the raw panax notoginseng powder while retaining the efficacy of the raw panax notoginseng powder. The method is simple, efficient, safe, inexpensive and economical, and has good development and application value.

Description

Technical Field

[0001] This invention relates to the technical field of sterilization methods for raw Panax notoginseng, and more particularly to a sterilization method for raw Panax notoginseng powder. Background Technology

[0002] Panax notoginseng powder is a major product in traditional Chinese medicine decoction pieces, with a very wide market coverage. However, the medicinal properties and therapeutic effects of Panax notoginseng powder vary depending on the processing method. The characteristic of its clinical application is that "raw Panax notoginseng is used to stop bleeding, promote blood circulation, reduce swelling, and relieve pain," earning it the reputation of a "holy medicine for trauma." In contrast, processed Panax notoginseng is used to replenish qi and blood, treating various deficiencies and injuries. The same medicinal material undergoes significant changes in its internal components and thus its efficacy varies considerably depending on the processing method. Literature reports indicate (Gao Yazhen et al. Effects of different steaming processes on the changes in effective components of Panax notoginseng, Chinese Journal of Experimental Traditional Medical Formulae, 2022, 28(3): 147-154) that after steaming, the chemical composition of raw Panax notoginseng undergoes significant changes, becoming cooked Panax notoginseng. Specifically, the content of notoginsenoside R1 and ginsenosides Rg1, Re, Rb1, and Rd decreases significantly, and new saponin components (such as ginsenosides Rh4, Rg3, Rk1, Rg5, and Rh2) are produced.

[0003] For the reasons mentioned above, to avoid changes in the effective components and impact on efficacy caused by high-temperature sterilization, irradiation sterilization is often used to sterilize raw Panax notoginseng powder. However, even irradiation sterilization significantly reduces the amount of ginsenoside Rg1 in raw Panax notoginseng powder. For example, Chinese invention patent CN111991576A discloses a sterilization method for Panax notoginseng powder, which eliminates microorganisms by irradiating the powder, but inevitably causes loss of the effective components in Panax notoginseng, making it unsuitable for sterilizing raw Panax notoginseng powder. Therefore, the sterilization of raw Panax notoginseng powder is a pressing and pressing issue that urgently needs to be addressed in the industry. Summary of the Invention

[0004] To solve the above-mentioned technical problems, the present invention aims to provide a sterilization method for raw Panax notoginseng powder. The sterilization method can kill microorganisms in raw Panax notoginseng powder at low temperature using ethanol vapor without changing the effective components. It is safe, efficient, simple and has good economic application value.

[0005] To achieve the above-mentioned technical effects, the present invention adopts the following technical solution:

[0006] In a first aspect, the present invention provides a method for sterilizing raw Panax notoginseng powder, comprising the following steps:

[0007] S1: Wash the raw Panax notoginseng medicinal material, dry it, and grind it into powder;

[0008] S2: Panax notoginseng powder is loaded into a rotating double cone jar and vacuumed until the pressure inside the jar is 0.2-0.3 kPa, preferably 0.2 kPa. This pressure is the minimum pressure that can be achieved inside the jar after vacuuming by a water-circulating vacuum pump, and it can also extract most of the air in the gaps between the powder.

[0009] S3: Fill the can with ethanol vapor until the pressure inside the can is 0.8-0.9 kPa; heat to 60-65°C and keep warm for 30-50 minutes, while rotating the can. During this process, the ethanol vapor fills the inside of the can and the gaps between the Panax notoginseng powder, achieving uniform sterilization without dead corners.

[0010] S4: Repeat step S3 2 to 3 times. The purpose is to remove the original ethanol vapor and add new ethanol vapor, which can increase the concentration of ethanol vapor. At the same time, through the suction and expansion of the vacuum, the living cells of microorganisms are ruptured, and more ethanol vapor enters the living cells of microorganisms, increasing the bactericidal power.

[0011] S5: Allow the temperature inside the tank to drop to room temperature, evacuate the tank until the pressure inside the tank is 0.2-0.3 kPa, fill the tank with clean air to release the vacuum, and rotate the tank for 20-40 minutes.

[0012] S6: Repeat step S5 above 2 to 3 times, replacing the ethanol vapor with clean air to obtain sterilized raw Panax notoginseng powder. At the same time, the replacement with clean air can reduce the ethanol residue after sterilization of raw Panax notoginseng powder.

[0013] Furthermore, the drying endpoint in S1 is that the moisture content of raw Panax notoginseng is not higher than 20%. That is, when the moisture content of raw Panax notoginseng powder is lower than 20%, the raw Panax notoginseng can be pulverized and then passed through a 120-200 mesh sieve to balance sterilization efficiency and sterilization effect.

[0014] Furthermore, the ethanol vapor is generated by heating 80% ethanol to improve the sterilization effect. Preferably, the ethanol vapor is generated by heating 80% ethanol to 90°C.

[0015] Furthermore, the clean air is filtered through a high-efficiency filter to remove particulate impurities from the air.

[0016] Furthermore, the clean air is sterilized by a sterilization device before being delivered into the tank. Preferably, the sterilization device employs any one of electric heating sterilization, electrostatic sterilization, and media filtration sterilization.

[0017] Furthermore, the sterilization equipment and the high-efficiency filter are connected in series.

[0018] Furthermore, the rotation speed of the tank in S3 is 30-50 r / min, preferably 30 r / min.

[0019] Secondly, the present invention provides an application of the above-mentioned sterilization method for raw Panax notoginseng powder in killing Gram bacteria and fungi in raw Panax notoginseng powder.

[0020] Furthermore, the Gram bacteria are any one of Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus.

[0021] Furthermore, the fungus includes Candida albicans.

[0022] Furthermore, the sterilization method for raw Panax notoginseng powder can also be used for the sterilization of pharmaceutical compositions containing raw Panax notoginseng powder or raw Panax notoginseng extract.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: the sterilization method for raw Panax notoginseng powder can quickly, efficiently, and easily sterilize the raw Panax notoginseng powder. It has been verified that the sterilization method for raw Panax notoginseng powder has a good killing effect on Gram bacteria including Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, as well as fungi including Candida albicans. More importantly, compared with irradiation sterilization equipment, the equipment used in the present invention is simple, easy to operate, and has a low sterilization cost. Furthermore, it effectively kills microorganisms in the raw Panax notoginseng powder while preserving the medicinal efficacy of the raw Panax notoginseng powder. The method is simple, efficient, safe, inexpensive, and economical, and has good development and application value. Detailed Implementation

[0024] The following embodiments are only used to more clearly illustrate the technical solutions of the present invention, and are therefore only examples, and should not be used to limit the scope of protection of the present invention. Unless otherwise specified, the test methods used in the following embodiments are conventional methods; the materials, reagents or instruments used, unless otherwise specified, are commercially available reagents and materials, and the conditions not specified in the embodiments are all carried out according to conventional conditions or conditions recommended by the manufacturer.

[0025] Example 1

[0026] This embodiment provides a sterilization method for raw Panax notoginseng powder, which is implemented by a rotating double-cone jar. Specifically, the method includes the following steps:

[0027] S1: Take 25kg of Panax notoginseng (80 heads), wash it, dry it with hot air circulation at 60℃ until its moisture content is 18% to 20%, and then grind it into fine powder;

[0028] S2: The sieved raw Panax notoginseng powder is loaded into the rotating double cone jar, and a vacuum is drawn until the pressure inside the jar is 0.2 kPa;

[0029] S3: Fill the tank with ethanol vapor until the pressure inside the tank is 0.8 kPa; heat to 65°C and keep at that temperature for 30 minutes, while rotating the tank during the heat preservation process;

[0030] S4: Repeat step S3 above;

[0031] S5: Reduce the temperature inside the tank to room temperature, evacuate the tank to a pressure of 0.2 kPa, fill the tank with clean air to release the vacuum, and rotate the tank for 20 minutes. The clean air must undergo sterilization and filtration before entering the tank.

[0032] S6: Repeat step S5 twice more, replacing the ethanol vapor with clean air to obtain sterilized raw Panax notoginseng powder.

[0033] After sterilizing the raw Panax notoginseng powder using the above method, the sterilized raw Panax notoginseng powder was tested according to the microbial testing method under the Powder section of the Chinese Pharmacopoeia (2020 edition). The test results showed that the sterilization effect met the requirements.

[0034] Example 3

[0035] This embodiment provides a specific example of a sterilization method for raw Panax notoginseng powder. The main purpose of this embodiment is to study the effect of different alcohol concentrations on the sterilization effect of raw Panax notoginseng powder. The specific process is as follows:

[0036] Studies have shown that the optimal concentration for alcohol sterilization is 75% ethanol. However, alcohol vapor sterilization and ethanol solution sterilization are not entirely the same. During the sterilization of traditional Chinese medicine powders and slices using alcohol vapor, the inventors discovered that although the types of microorganisms killed were the same (Escherichia coli, Candida albicans, etc.), the alcohol concentration varied depending on the material. Due to differences in alcohol concentration and heating temperature, the ethanol content in the alcohol vapor also varied, resulting in different sterilization effects. For the vacuum alcohol vapor sterilization of Panax notoginseng powder, the following screening was conducted on the alcohol concentration:

[0037] 2.1 Take 70kg of Panax notoginseng (80 heads), wash it, dry it with hot air circulation at 60℃, grind it into fine powder, and divide it into 7 portions; add 50ml of mixed solution containing Escherichia coli and Candida albicans to each portion;

[0038] 2.2 Sterilization was performed using the methods and procedures described in Example 1. The sterilization effect of alcohol vapor produced at different alcohol concentrations on Panax notoginseng powder was investigated. The alcohol concentrations were set at 65%, 70%, 75%, 80%, 85%, 90%, and 95%.

[0039] 2.3 Detection Methods

[0040] The Lüssler alkaline methylene blue staining method was used. Each sample was tested three times, with five fields of view (1000x magnification) taken each time, for a total of 15 fields of view. The number of unstained bacteria (live bacteria) in each field of view was observed, and the unsterilized sample was used as a control for statistical analysis.

[0041] 2.4 Experimental results are shown in Table 1

[0042] Table 1. Effects of different alcohol concentrations on microbial killing.

[0043]

[0044] Note: The above data represents the number of fields of view showing live bacteria.

[0045] The above experimental results show that the alcohol vapor produced by 80% alcohol has the best sterilization effect on Panax notoginseng powder, and can completely kill it.

[0046] Example 3

[0047] This embodiment provides a specific example of a sterilization method for raw Panax notoginseng powder. This embodiment refers to the content determination method in the "Panax notoginseng" quality standard included in the Chinese Pharmacopoeia, and determines the content of raw Panax notoginseng powder prepared using different sterilization methods: Panax notoginseng medicinal material is taken, washed, dried, pulverized into fine powder, and mixed evenly; four portions are taken, each weighing 5 kg. The first portion is a control sample (no sterilization treatment), the second portion is sterilized by steam at 121℃ for 30 min, and the third portion is sterilized by C... O 60 -γ irradiation of 10 kGy, the fourth part is the method of this invention, and the experimental results of each group are shown in Table 2:

[0048] Table 2 Comparison of sterilization effects among groups

[0049] sample 1 (Control sample) 2 (Steam sterilization) 3 (Irradiation sterilization) 4 (Inventive Method) Panax notoginsenoside R1 1.16％ 0.99％ 1.11％ 1.17％ Ginsenoside Rg1 2.98％ 2.11％ 2.50％ 2.95％ Ginsenoside Rb1 2.54％ 1.82％ 2.42％ 2.56％ Ginsenoside Re 0.52％ 0.58％ 0.55％ 0.56％ Ginsenoside Rd 0.64％ 0.51％ 0.65％ 0.62％

[0050] The above experimental results show that high-temperature steam and irradiation sterilization both cause the active ingredients of Panax notoginseng powder to deteriorate. However, the method of the present invention can not only kill the microorganisms but also ensure that the active ingredients do not change, thereby ensuring the efficacy of Panax notoginseng powder.

[0051] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the present invention, and all such modifications and substitutions should be covered within the scope of the claims of the present invention. Technical aspects, shapes, and structures not described in detail in this invention are all well-known technologies.

Claims

1. A method for sterilizing raw Panax notoginseng powder, characterized in that, Includes the following steps: S1: Wash the raw Panax notoginseng, dry it, and grind it into powder; S2: Panax notoginseng powder is loaded into a rotating double cone jar, and a vacuum is drawn until the pressure inside the jar is 0.2-0.3 kPa; S3: Fill the tank with ethanol vapor until the pressure inside the tank is 0.8-0.9 kPa; heat to 60-65°C, keep at that temperature for 30-50 minutes, and rotate the tank at the same time; S4: Repeat step 2-3 of S3; S5: Reduce the temperature inside the tank to room temperature, evacuate the tank to a pressure of 0.2-0.3 kPa, fill the tank with clean air to release the vacuum, and rotate the tank for 20-40 minutes. S6: Repeat step S5 above 2-3 times, replacing the ethanol vapor with clean air to obtain sterilized raw Panax notoginseng powder; The ethanol vapor is generated from 80% ethanol.

2. The sterilization method for raw Panax notoginseng powder as described in claim 1, characterized in that: The drying endpoint in S1 is that the moisture content of raw Panax notoginseng is not higher than 20%.

3. The sterilization method for raw Panax notoginseng powder as described in claim 1, characterized in that: The clean air is filtered through a high-efficiency filter.

4. The sterilization method for raw Panax notoginseng powder as described in claim 1, characterized in that: The rotation speed of the tank in S3 is 30-50 r / min.

5. The application of the sterilization method for raw Panax notoginseng powder as described in any one of claims 1 to 4 in killing Gram bacteria and fungi in raw Panax notoginseng powder.

6. The application as described in claim 5, characterized in that: The Gram bacteria mentioned are any one of Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus.

7. The application as described in claim 5, characterized in that: The fungi include Candida albicans.

8. As described in claim 5, the sterilization method for raw Panax notoginseng powder can also be used for the sterilization of pharmaceutical compositions containing raw Panax notoginseng powder or raw Panax notoginseng extract.

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