Cladosporium tenuissima NXY8, mycelial pellets and their application in high-salinity wastewater treatment

Abstract

The present invention relates to the technical field of biological wastewater treatment, and in particular to Cladosporium NXY8, mycelial pellets and their application in high-salt wastewater treatment. The Cladosporium NXY8 provided by the present invention is a salt-tolerant bacterium, which is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No. 40141. The Cladosporium NXY8 can form mycelial pellets with salt tolerance, and can be used in the organic matter (COD) degradation treatment process of salt-containing wastewater with a salinity of 0 to 10 wt.%; at the same time, the mycelial pellets formed by the fungus have a sedimentation rate of 0.4 to 2.5 cm / s, which is much higher than ordinary activated sludge flocs. The independent structure of the mycelial pellets is easy to separate solids and liquids, and can be separated by simple sedimentation or filtration, reducing the construction of secondary sedimentation tanks.

Description

Technical Field

[0001] The invention relates to the technical field of biological wastewater treatment, in particular to Cladosporium tenuissima NXY8, mycelial pellets and application thereof in high-salt wastewater treatment. Background Art

[0002] Salty organic wastewater is common in different industries such as oil refining, mining, textiles, chemicals, pharmaceuticals, and food processing. The direct use of seawater in coastal areas for life and production, and the infiltration of seawater into freshwater layers have also exacerbated the discharge of salty organic wastewater. The discharge of these wastewaters will destroy the balance and stability of the ecosystem and will also corrode metal pipes and other equipment.

[0003] Before desalting saline organic wastewater, the organic matter in it needs to be treated. Finding suitable saline organic wastewater treatment technology is in line with the development goals of national ecological environment construction. At present, advanced oxidation methods such as electrocatalytic oxidation, Fenton oxidation, ozone oxidation, etc. are mainly used for this type of wastewater, but they have problems such as high energy consumption and reagent costs, and large equipment investment. Compared with advanced oxidation methods, biological oxidation (including activated sludge method) has the advantages of low cost, easy scalability, low carbon and environmental protection.

[0004] However, the sludge produced by the activated sludge process is difficult to separate from water, requires a secondary sedimentation tank, and has poor resistance to stress in saline environments. Summary of the invention

[0005] In order to solve the above problems, the present invention provides Cladosporium NXY8, mycelial pellets and their use in high-salt wastewater treatment. The Cladosporium NXY8 provided by the present invention has salt tolerance, can not only effectively degrade organic matter in saline wastewater, but also its mycelial pellets can adsorb activated sludge, accelerate sedimentation, and reduce the construction of secondary sedimentation tanks.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The invention provides a Cladosporium tenuissimum NXY8 which can form balls. The deposit number of the Cladosporium tenuissimum NXY8 is CGMCC No.40141.

[0008] The present invention also provides a mycelium pellet which can reduce or assist in reducing COD in wastewater. The mycelium pellet is obtained by culturing the Cladosporium tenuissima NXY8.

[0009] The present invention also provides a method for preparing the mycelial pellets, comprising inoculating a spore suspension containing the Cladosporium tenuissima NXY8 into a culture medium, culturing, and obtaining the mycelial pellets;

[0010] The components of the culture medium include: 5-80 g / L glucose, 0.5-8 g / L NH4Cl, 0.3-6 g / L KH2PO4, 0.25-4 g / L MgSO4·7H2O and 0-100 g / L NaCl.

[0011] Preferably, the culture conditions include: temperature 15-35°C, time 40-240h, and rotation speed 100-400rpm.

[0012] Preferably, the volume ratio of the spore suspension to the culture medium is 1:99; the spore concentration of the spore suspension is 10 4 ~10 9 spores / mL.

[0013] Preferably, the method for preparing the spore suspension comprises:

[0014] The Cladosporium tenuissima NXY8 described in the above scheme is inoculated on a PDA medium, and after slant culture, the spores are washed into sterile physiological saline to obtain the spore suspension.

[0015] Preferably, the conditions for the slant culture include: a temperature of 15 to 35° C. and a time of 2 to 10 days.

[0016] The present invention also provides the use of the Cladosporium tenuissima NXY8 or the mycelial pellet or the mycelial pellet prepared by the above method in reducing or assisting in reducing COD in wastewater.

[0017] Preferably, the wastewater comprises saline organic wastewater.

[0018] Preferably, the wastewater includes one or more of municipal sewage, mustard wastewater, fishery pickling wastewater, oil and gas field wastewater, chemical industry wastewater, light industry wastewater and prepared simulated wastewater.

[0019] Beneficial effects:

[0020] The present invention provides a Cladosporium tenuissimum NXY8 that can form a ball, and the deposit number of the Cladosporium tenuissimum NXY8 is CGMCC No. 40141. The Cladosporium tenuissimum NXY8 provided by the present invention is a salt-tolerant bacterium that can form a mycelium ball with salt tolerance and can be used in the organic matter degradation treatment process of saline wastewater with a salinity of 0 to 10 wt.%; at the same time, the mycelium ball formed by the fungus has a sedimentation rate of 0.4 to 2.5 cm / s, which is much higher than that of ordinary activated sludge flocs. The independent structure of the mycelium ball can make solid-liquid separation easier, and can be separated by a simple sedimentation or filtration method, reducing the construction of a secondary sedimentation tank.

[0021] Furthermore, the Cladosporium NXY8 provided by the present invention can be cultivated to obtain a large number of mycelial pellets with high biological activity. The mycelial pellets are added to the wastewater treatment reactor to quickly exert degradation and adsorption effects. Compared with the longer adaptation time of the ordinary activated sludge method in a salinity environment, after adding this strain, the system can quickly achieve a higher organic matter removal rate, the acclimation time is short, and the biochemical process debugging time is shortened.

[0022] Furthermore, the mycelial pellets formed by Cladosporium NXY8 provided by the present invention can be used as carriers to adsorb functional microorganisms or activated sludge to jointly treat saline organic wastewater. Compared with the mycelial pellets acting independently, saline organic wastewater with a higher organic load can be treated.

[0023] Biological Deposit Description

[0024] Cladosporium tenuissimum NXY8, with the Latin name Cladosporium tenuissimum, was deposited in the China General Microbiological Culture Collection on March 4, 2022. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the deposit number is CGMCC No.40141. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0026] Figure 1 The colony morphology of NXY8 strain on culture medium and its morphological characteristics under microscope;

[0027] Figure 2 is the phylogenetic tree of NXY8 strain;

[0028] Figure 3 The mycelial ball morphology prepared in Example 2;

[0029] Figure 4 The mycelial ball morphology prepared in Example 3;

[0030] Figure 5 The mycelial ball morphology prepared in Example 4;

[0031] Figure 6 The mycelial ball morphology prepared in Example 5;

[0032] Figure 7 The mycelial ball morphology prepared in Example 6;

[0033] Figure 8 The mycelial ball morphology prepared in Example 7;

[0034] Fig. 9The mycelial ball morphology prepared in Comparative Example 1;

[0035] Fig.10 This is the morphology of the mycelium ball prepared in Comparative Example 2. DETAILED DESCRIPTION

[0036] The invention provides a Cladosporium tenuissimum NXY8 which can form balls. The deposit number of the Cladosporium tenuissimum NXY8 is CGMCC No.40141.

[0037] The spores of Cladosporium tenuissima NXY8 of the present invention are in the shape of an elongated lemon, and the spores are all terminally grown without being lateral.

[0038] After the Cladosporium NXY8 of the present invention is cultured on a CMA medium, the colonies of the strain are velvety, flat, light green, and later pale white and hair-like; after being cultured on a PDA medium, the colonies of the Cladosporium NXY8 strain are milky white and velvety against a light yellow PDA medium background, with wrinkles radiating from the middle to the edge, and a circle of dark colonies on the edge.

[0039] The Cladosporium tenuissima NXY8 of the present invention can tolerate a salinity of 0 to 10 wt.%, and can effectively reduce the organic matter content (COD) in saline wastewater in different salinity ranges.

[0040] In the present invention, the nucleotide sequence of ITS rDNA of Cladosporium exigua NXY8 preferably includes the sequence shown in SEQ ID NO.1, which is as follows:

[0041] .

[0042] The Cladosporium microsporum NXY8 of the present invention has the characteristic of being able to form balls, has a better separation effect during mud-water separation, requires a short sedimentation time, can save separation time, correspondingly increases reaction time and influent organic load, and improves treatment efficiency; at the same time, can reduce the construction of a secondary sedimentation tank, can perform simple separation, is suitable for removing or assisting in removing pollutants in wastewater, in particular can remove or assist in removing organic matter in wastewater, and is particularly suitable for a saline wastewater treatment process.

[0043] The present invention also provides a mycelial pellet that can reduce or assist in reducing COD in wastewater. The mycelial pellet is obtained by culturing the Cladosporium tenuissima NXY8 described in the above scheme.

[0044] The mycelium ball of the present invention is spherical, ellipsoidal, mallet-shaped or radially spherical, presenting white or different shades of green, with a diameter of 100 to 5000 μm; and the sedimentation speed of the mycelium ball is 0.4 to 2.5 cm / s.

[0045] The mycelium ball of the present invention has the characteristics of easy solid-liquid separation, fast sedimentation speed, strong organic matter degradation and adsorption capacity, can independently adsorb and degrade organic matter in wastewater, and the unit biomass organic matter treatment load is 0.01-2kg COD / (kg MLSS·d), or adsorb activated sludge to jointly degrade organic matter in wastewater, and the unit biomass organic matter treatment load is 0.01-4kg COD / (kg MLSS·d), which solves the problem that the activated sludge method requires a secondary sedimentation tank for mud-water separation, can reduce the construction of the secondary sedimentation tank, and solves the problem of floating and disintegration of activated sludge in a high-salt environment, and has significant application potential in the field of saline wastewater treatment.

[0046] The present invention also provides a method for preparing the mycelial pellets described in the above scheme, wherein a spore suspension containing Cladosporium tenuissima NXY8 described in the above scheme is inoculated into a culture medium, and cultured to obtain the mycelial pellets.

[0047] In the present invention, the method for preparing the spore suspension preferably comprises:

[0048] 1) using an inoculating loop to streak the Cladosporium tenuissima NXY8 on a sterile potato dextrose agar (PDA) medium slant;

[0049] 2) placing the slope marked in step 1) in an incubator at 15 to 35° C. for 2 to 10 days;

[0050] 3) Wash the spores on the slope in step 2) into sterile physiological saline to prepare a spore suspension.

[0051] In the present invention, the culture temperature in step 2) is preferably 25-32°C, more preferably 30°C; the culture time is preferably 2-10 days, more preferably 5-6 days or 8-10 days, more preferably 8 days.

[0052] After obtaining the spore suspension, the present invention inoculates the spore suspension into a culture medium and cultures the culture medium to obtain the mycelium ball.

[0053] In the present invention, the volume ratio of the spore suspension to the culture medium is preferably 1:99; the spore concentration of the spore suspension is preferably 10 4 ~10 9 spores / mL, further preferably including 10 4 , 10 5 , 5×10 5 , 10 6 , 2×10 6 , 10 7 , 10 8 , 10 9 spores / mL, more preferably 10 6The invention can cultivate mycelium balls with moderate volume, complete structure and fast sedimentation speed through appropriate inoculation amount.

[0054] The culture medium of the present invention comprises 5 to 80 g / L of glucose, preferably 10 to 50 g / L, more preferably 15 to 20 g / L, and more preferably 15 g / L. In the present invention, the glucose preferably comprises anhydrous glucose.

[0055] The culture medium of the present invention comprises 0.5-8 g / L of NH4Cl, preferably 0.7-5 g / L, more preferably 0.8-4 g / L, and more preferably 1.5 g / L.

[0056] The culture medium of the present invention comprises KH2PO4 0.3-6 g / L, preferably 0.5-2 g / L, more preferably 0.6-1 g / L, and more preferably 0.9 g / L.

[0057] The culture medium of the present invention comprises MgSO4·7H2O 0.25-4 g / L, preferably 0.4-2 g / L, more preferably 0.6-1 g / L, and more preferably 0.7 g / L.

[0058] The culture medium of the present invention includes 0-100 g / L of NaCl; the concentration of NaCl in the culture medium is preferably determined according to the salinity of the wastewater to be treated, specifically including: the value before the percentage of the salinity of the wastewater to be treated is recorded as A, for example, when the salinity is 10%, A is 10, and the concentration of NaCl in the culture medium = A×10 g / L; wherein when A is less than 1, NaCl may not be added to the culture medium. The present invention determines the concentration of NaCl in the culture medium according to the salinity of the wastewater to be treated, so that the mycelial balls obtained by culture can directly play the role of degrading and adsorbing organic matter in saline wastewater, without the need for an adaptive acclimation process, thereby shortening the debugging time of the biochemical process.

[0059] In the present invention, the culture preferably includes constant temperature culture; the culture temperature is preferably 15-35°C, preferably 20-32°C, and further more preferably 28°C; the culture time is preferably 40-240h, further preferably 50-120h or 144-192h, and more preferably 112h; the culture rotation speed is preferably 100-400rpm, further preferably 100-150rpm, or 160-190rpm, and more preferably 180rpm.

[0060] The present invention also provides the use of the Cladosporium tenuissima NXY8 or the mycelial pellet or the mycelial pellet prepared by the above method in reducing or assisting in reducing COD in wastewater.

[0061] In the present invention, the wastewater preferably includes saline organic wastewater, and more preferably high-salt wastewater; the saline organic wastewater preferably includes one or more of municipal sewage, mustard wastewater, fishery pickling wastewater, oil and gas field wastewater, chemical industry wastewater, light industry wastewater and prepared simulated wastewater.

[0062] In the present invention, the mycelium pellet is preferably used as a carrier to adsorb activated sludge or other functional microorganisms and degrade organic matter in liquid, and is particularly suitable for organic matter in saline wastewater.

[0063] In order to further illustrate the present invention, the Cladosporium tenuissima NXY8, mycelial pellets and their application in high-salt wastewater treatment provided by the present invention are described in detail below in conjunction with the examples, but they should not be construed as limiting the scope of protection of the present invention.

[0064] The method for determining the sedimentation velocity of the following embodiments or comparative examples is as follows: sodium chloride solution is filled into a 1000 mL measuring cylinder, 30 mycelial balls are randomly selected, and one mycelial ball is released from the top of the liquid surface without applying an initial velocity each time, and the time it takes to settle to the bottom of the measuring cylinder is recorded, and the sedimentation velocity is calculated, and the average value is taken to obtain the average sedimentation velocity of the mycelial balls in the sodium chloride solution.

[0065] Example 1

[0066] Isolation and Identification of NXY8 from Cladosporium tenuissimum

[0067] The Cladosporium tenuissima NXY8 described in the present invention was isolated from the seaside soil in Fengxian District, Shanghai. The details are as follows:

[0068] Prepare a high-salt liquid culture medium (containing 20 g / L glucose, 1 g / L NH4Cl, 1 g / L KH2PO4, 0.8 g / L MgSO4·7H2O, 1 g / L yeast powder, and 50 g / L NaCl). Crush the soil and shake it with clean water, filter it through a single layer of gauze, add 10 mL of the extract to 100 mL of high-salt liquid culture medium, and after one day of enrichment culture, take 10 mL and transfer it to 100 mL of new high-salt liquid culture medium, and repeat the enrichment for 5 times, each time for 2 days.

[0069] Agar was added to the high-salt liquid medium (the mass volume ratio of agar to high-salt liquid medium was 20 g:1 L), and a high-salt glucose agar plate was prepared after sterilization; the bacterial liquid in the high-salt liquid medium after enrichment for 5 times was dipped, and the high-salt glucose agar plate was streaked for separation, and the fungal strain was obtained after multiple purifications, which was numbered as NXY8. Then, the spores were washed, diluted and spread to obtain a single fungal colony.

[0070] The fungal colonies were inoculated onto corn meal agar (CMA) medium and potato dextrose agar (PDA) medium respectively and cultured in an incubator at 30°C for 10 days. Figure 1 As shown, A to C are the results of the strain cultured in CMA medium, D to F are the results of the strain cultured in PDA medium, A and D are the front side of the culture medium, B and E are the back side of the culture medium, and C and F are slide photos under a microscope.

[0071] After culturing on CMA medium, the colonies of this strain are velvety, flat, light green, and later pale white and hairy. After culturing on PDA medium, the colonies of this strain appear milky white and velvety against a light yellow PDA medium background, with wrinkles radiating from the middle to the edge, and a circle of dark colonies on the edge.

[0072] The structure of the NXY8 strain under a microscope is shown below: Figure 1 As shown in C and F in Figure 1, the spores are slender lemon-shaped, and all spores are terminal and not lateral.

[0073] The genomic DNA of strain NXY8 was extracted using a fungal genomic DNA extraction kit and used as a template for ITS rDNA amplification. PCR amplification was performed using the universal fungal primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3', SEQ ID NO.2) and ITS4 (5'-TCCTCCGCTTATTGATATGC-3', SEQ ID NO.3). The PCR reaction system (20 μL) included 10× buffer 2 μL, dNTP Mix (2.5 mM) 1.6 μL, ITS1 and ITS4 0.8 μL each, DNA template 0.5 μL, Taq enzyme (5 U / μL) 0.2 μL, and sterile water 14.1 μL. The PCR reaction procedure was as follows: 95°C pre-denaturation for 5 min; 95°C denaturation for 30 s, 55°C annealing for 30 s, 72°C extension for 40 s, 35 cycles; 72°C post-extension for 10 min.

[0074] The PCR product obtained by amplification was recovered by 1% agarose electrophoresis, and purified and sequenced by Beijing Liuhe BGI Technology Co., Ltd. The ITS rDNA sequencing result of the NXY8 strain is shown in SEQ ID NO.1. The obtained sequence was compared with the GenBank DNA sequence database of NCBI for homology analysis, and the results showed that the obtained strain had a high homology with the sequence of Cladosporium tenuissimum isolate 42 (MK311278.1). The phylogenetic tree was constructed by the neighbor-joining method, as shown in Figure 2As shown. Based on the colony and spore morphology, homology comparison and phylogenetic analysis, the strain was identified as Cladosporium tenuissimum and named Cladosporium tenuissimum NXY8. The strain was deposited in the General Microbiology Center (CGMCC) of the China Microbiological Collection Administration Committee in Chaoyang District, Beijing on March 4, 2022, with the deposit number CGMCC No.40141.

[0075] Example 2

[0076] A mycelial ball for removing or assisting in removing COD in high-salinity wastewater is prepared by the following method:

[0077] 1) taking out the glycerol tube containing the Cladosporium NXY8 strain described in Example 1 from a -80°C refrigerator, and using an inoculating loop to make a Z-shaped streak on the slant of a sterile PDA medium; wherein the sterile PDA medium is prepared by adding deionized water to 1 L, 15 g of potato extract powder, 20 g of anhydrous glucose and 15 g of agar, and then sterilizing;

[0078] 2) Place the marked slope in an incubator at 30°C for 8 days;

[0079] 3) Wash the spores on the slope into sterile saline in a clean bench to prepare a spore suspension, and count the number of spores per milliliter of the spore suspension under a microscope using a hemocytometer; adjust the spore concentration of the spore suspension to 10 with sterile saline. 9 spores / mL;

[0080] 4) inoculating 1 mL of the spore suspension into a shake flask containing 99 mL of sterile culture medium; wherein the sterile culture medium is prepared by adding deionized water to 1 L, 15 g of anhydrous glucose, 1.5 g of NH4Cl, 0.9 g of KH2PO4, 0.7 g of MgSO4·7H2O and 30 g of NaCl, and sterilizing;

[0081] 5) The shake flask inoculated with the spore suspension was placed in a shaker and cultured at 28° C. and 180 rpm for 112 h to obtain mycelial balls.

[0082] like Figure 3 As shown, the mycelium balls are spherical, brown-green, with an average diameter of 1470 μm and an average sedimentation velocity of 0.74 cm / s in 30 g / L NaCl solution.

[0083] Example 3

[0084] A mycelial ball for removing or assisting in removing COD in high-salinity wastewater is prepared by the following method:

[0085] Steps 1) to 5) are similar to those in Example 2, except that the spore concentration of the spore suspension in step 3) is 10 8 spores / mL.

[0086] like Figure 4 As shown, the mycelium balls are spherical, light green, with an average diameter of 1778 μm, and an average sedimentation velocity of 0.87 cm / s in 30 g / L NaCl solution.

[0087] Example 4

[0088] A mycelial ball for removing or assisting in removing COD in high-salinity wastewater is prepared by the following method:

[0089] Steps 1) to 5) are similar to those in Example 2, except that the spore concentration of the spore suspension in step 3) is 10 7 spores / mL.

[0090] like Figure 5 As shown, the mycelium balls are spherical, light yellow, with an average diameter of 2924 μm and an average sedimentation velocity of 1.36 cm / s in 30 g / L NaCl solution.

[0091] Example 5

[0092] A mycelial ball for removing or assisting in removing COD in high-salinity wastewater is prepared by the following method:

[0093] Steps 1) to 5) are similar to those in Example 2, except that the spore concentration of the spore suspension in step 3) is 10 6 spores / mL.

[0094] like Figure 6 As shown, the mycelium balls are spherical and white, with an average diameter of 2696 μm and an average sedimentation velocity of 1.19 cm / s in 30 g / L NaCl solution.

[0095] Example 6

[0096] A mycelial ball for removing or assisting in removing COD in high-salinity wastewater is prepared by the following method:

[0097] Steps 1) to 5) are similar to those of Example 5, with the only difference being that the sterile culture medium in step 4) is prepared by adding deionized water to 1 L to 15 g of anhydrous glucose, 1.5 g of NH4Cl, 0.9 g of KH2PO4, 0.7 g of MgSO4·7H2O and 100 g of NaCl, followed by sterilization.

[0098] like Figure 7As shown, the mycelium balls are spherical, white, with an average diameter of 2854 μm, and an average sedimentation velocity of 1.05 cm / s in 100 g / L NaCl solution.

[0099] Example 7

[0100] A mycelial ball for removing or assisting in removing COD in high-salinity wastewater is prepared by the following method:

[0101] Steps 1) to 5) are similar to those of Example 5, with the only difference being that the sterile culture medium in step 4) is prepared by adding deionized water to 1 L to 15 g of anhydrous glucose, 1.5 g of NH4Cl, 0.9 g of KH2PO4 and 0.7 g of MgSO4·7H2O, followed by sterilization.

[0102] like Figure 8 As shown, the mycelium balls are spherical, dark green, with an average diameter of 2590 μm, and an average sedimentation velocity of 1.42 cm / s in 0 g / L NaCl solution.

[0103] Comparative Example 1

[0104] A fungus cultivation method, comprising the following steps:

[0105] Steps 1) to 5) are similar to those in Example 5, except that the shaking speed in step 5) is 20 rpm. Fig. 9 ).

[0106] Comparative Example 2

[0107] A fungus cultivation method, comprising the following steps:

[0108] Steps 1) to 5) are similar to those in Example 5, except that the sterile culture medium in step 4) is prepared by adding deionized water to 1L to 15g of anhydrous glucose, 1.5g of NH4Cl, 0.9g of KH2PO4, 0.7g of MgSO4·7H2O and 140g of NaCl, and then sterilizing. Fig.10 ).

[0109] Application Example 1

[0110] The mycelium pellets prepared in Example 2 were added to a shaking bottle containing coal chemical wastewater (salinity: 3 wt.%, COD concentration: 1200 mg / L) at a dosage of 2 g / L, and placed in a shaking table at 25°C and 150 rpm for 72 hours. The COD removal rate reached 72%.

[0111] Application Example 2

[0112] The mycelium pellets prepared in Example 3 were added to coal chemical wastewater (salinity of 3wt.%, COD concentration of 1200mg / L), with a dosage of 2g / L, and a cylindrical reactor (height-to-diameter ratio of 3) was operated in a sequencing batch mode, with a cycle of 6h, a water replacement ratio of 50%, a pH maintained at 6.0-8.0, a temperature maintained at 25℃-30℃, and a dissolved oxygen concentration controlled at 8-10mg / L. The organic load in the system was 2.4kg COD / (m 3 ·d).

[0113] After the first day, the organic matter removal rate can reach 50%; after the fifth day, the organic matter removal rate reaches 82%. At this time, the mycelium balls act as carriers to adsorb functional microorganisms to jointly treat saline organic wastewater, and the total biomass reaches 3g / L.

[0114] Application Example 3

[0115] The mycelium pellets prepared in Example 4 were added to the diluted mustard wastewater (salinity of 3wt.%, COD concentration of 2000mg / L), the dosage was 3g / L, and the cylindrical reactor (height-to-diameter ratio of 3) was operated in a sequencing batch mode, with a cycle of 8h, a water replacement ratio of 50%, pH maintained at 6.0-8.0, a temperature maintained at 25℃-30℃, and a dissolved oxygen concentration controlled at 8-10mg / L. The organic load in the system was 3kg COD / (m 3 ·d).

[0116] After the first day, the organic matter removal rate can reach 60%; after the eighth day, the organic matter removal rate reaches 95%. At this time, the mycelium balls act as carriers to adsorb functional microorganisms to jointly treat saline organic wastewater, and the total biomass reaches 4.3g / L.

[0117] Application Example 4

[0118] The mycelium pellets prepared in Example 5 were added to the pickling wastewater (salinity of 3wt.%, COD concentration of 2200mg / L), the dosage was 3g / L, and the cylindrical reactor (height-to-diameter ratio of 3) was operated in a sequencing batch mode, with a cycle of 8h, a water replacement ratio of 50%, pH maintained at 6.0-8.0, a temperature maintained at 25℃-30℃, and a dissolved oxygen concentration controlled at 8-10mg / L. The organic load in the system was 3.3kg COD / (m 3 ·d).

[0119] After the first day, the organic matter removal rate can reach 40%; after the seventh day, the organic matter removal rate reaches 85%. At this time, the mycelium balls act as carriers to adsorb functional microorganisms to jointly treat saline organic wastewater, and the total biomass reaches 3.8g / L.

[0120] Application Example 5

[0121] The mycelium pellets prepared in Example 6 were added to mustard wastewater (salinity of 10wt.%, COD concentration of 4500mg / L), with a dosage of 4g / L, and a cylindrical reactor (height-to-diameter ratio of 3) was operated in a sequencing batch mode, with a cycle of 18h, a water replacement ratio of 50%, a pH maintained at 6.0-8.0, a temperature maintained at 25℃-30℃, and a dissolved oxygen concentration controlled at 8-10mg / L. The organic load in the system was 6kgCOD / (m 3 ·d).

[0122] After the first day, the organic matter removal rate can reach 30%; on the 8th day, the organic matter removal rate gradually increased to 75%; on the 15th day, the organic matter removal rate stabilized at 80%. At this time, the mycelium balls acted as carriers to adsorb functional microorganisms to jointly treat saline organic wastewater, and the total biomass reached 5.5g / L.

[0123] Application Example 6

[0124] The mycelium pellets prepared in Example 7 were added to the industrial park wastewater (salinity of 0.5wt.%, COD concentration of 800mg / L), the dosage was 2g / L, and the cylindrical reactor (height-to-diameter ratio of 3) was operated in a sequencing batch mode, with a cycle of 6h, a water replacement ratio of 50%, pH maintained at 6.0-8.0, a temperature maintained at 25℃-30℃, and a dissolved oxygen concentration controlled at 8-10mg / L. The organic load in the system was 1.6kg COD / (m 3 ·d).

[0125] After the first day, the organic matter removal rate could reach 38%; on the 6th day, the organic matter removal rate gradually increased to 70%; on the 15th day, the organic matter removal rate stabilized at 85%. At this time, the mycelial balls acted as carriers to adsorb functional microorganisms to jointly treat saline organic wastewater, and the total biomass reached 3.5g / L.

[0126] In summary, the Cladosporium tenuissima NXY8 provided by the present invention can be used in the process of treating saline wastewater, which can not only effectively remove organic matter in the saline wastewater, but also adsorb functional microorganisms and reduce the construction of secondary sedimentation tanks.

[0127] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A ball-forming Cladosporium Cladosporium tenuissimum )NXY8, characterized in that, The deposit number of Cladosporium tenuissima NXY8 is CGMCC No.40141.

2. A mycelial pellet that can reduce or assist in reducing COD in wastewater, characterized in that: The mycelial pellet is obtained by culturing the Cladosporium tenuissima NXY8 described in claim 1.

3. The method for preparing mycelial pellets according to claim 2, characterized in that: Inoculating a spore suspension containing Cladosporium tenuissima NXY8 according to claim 1 into a culture medium, culturing, and obtaining the mycelial pellet; The components of the culture medium include: 5-80 g / L glucose, 0.5-8 g / L NH4Cl, 0.3-6 g / L KH2PO4, 0.25-4 g / L MgSO4·7H2O and 0-100 g / L NaCl.

4. The preparation method according to claim 3, characterized in that: The culture conditions include: temperature 15-35° C., time 40-240 h, and rotation speed 100-400 rpm.

5. The preparation method according to claim 3 or 4, characterized in that: The volume ratio of the spore suspension to the culture medium is 1:99; the spore concentration of the spore suspension is 10 4 ~10 9 spores / mL.

6. The preparation method according to claim 3 or 4, characterized in that: The preparation method of the spore suspension comprises: The Cladosporium tenuissima NXY8 is inoculated on a PDA medium, and after slant culture, the spores are washed into sterile physiological saline to obtain the spore suspension.

7. The preparation method according to claim 6, characterized in that: The conditions for the slant culture include: a temperature of 15-35° C. and a time of 2-10 days.

8. Use of the Cladosporium microsporum NXY8 described in claim 1 or the mycelial pellets described in claim 2, or the mycelial pellets prepared by the method described in any one of claims 3 to 7 in reducing or assisting in reducing COD in wastewater; the wastewater is one or more of mustard wastewater, fishery pickling wastewater and coal chemical industry wastewater.

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