Antigen concentration measurement method and measuring instrument

Abstract

This application discloses a method and instrument for measuring antigen concentration. The method comprises: diluting at least a portion of a first sample containing an antigen to obtain a second sample; reacting the second sample in a first accommodating space and then measuring the concentration to determine whether the antigen concentration in the second sample exceeds a specified limit; if the antigen concentration in the second sample exceeds a specified limit, diluting the second sample to obtain a third sample containing the antigen; reacting the third sample in the first accommodating space and then measuring the concentration. This method can reduce the cost of measuring antigen concentration.

Description

Technical Field

[0001] The present application relates to the field of clinical testing technology, and in particular to a method and instrument for measuring antigen concentration. Background Art

[0002] In many application scenarios, it is necessary to detect the concentration of antigens contained in samples. However, existing detection conditions are insufficient to support the measurement of antigen concentrations across all numerical ranges. That is, when the antigen concentration in a sample exceeds the standard, the concentration of the antigen in the sample cannot be detected. Therefore, the method for detecting the concentration of antigens contained in a sample is to first measure the concentration of the antigen contained in the sample to see if it exceeds the standard. If the antigen concentration does not exceed the standard, the concentration of the antigen contained in the sample is determined. Existing methods for measuring antigen concentrations are costly. Summary of the Invention

[0003] The present application provides an antigen concentration measurement method and a measuring instrument, which can reduce the cost required for antigen concentration measurement.

[0004] To address the above technical issues, the present application adopts a technical solution: providing a method for measuring antigen concentration. The method comprises: diluting at least a portion of a first sample containing an antigen to obtain a second sample; reacting the second sample in a first accommodating space and then measuring the concentration to determine whether the antigen concentration in the second sample exceeds a standard; if the antigen concentration in the second sample exceeds a standard, diluting the second sample to obtain a third sample containing the antigen; and reacting the third sample in the first accommodating space and then measuring the concentration.

[0005] To solve the above technical problems, another technical solution adopted by this application is: providing a measuring instrument, which includes a power component, a measuring component, and a control circuit, and is provided with a first accommodating space, wherein the control circuit is used to control the power component to dilute a first sample containing an antigen to obtain a second sample; after the second sample reacts in the first accommodating space, the measuring component is controlled to measure to determine whether the antigen concentration in the second sample exceeds the standard; if the antigen concentration in the second sample exceeds the standard, the control circuit is used to control the power component to dilute to obtain a third sample containing the antigen; after the third sample reacts in the first accommodating space, the measuring component is controlled to measure.

[0006] In the above manner, in the process of measuring the antigen concentration of the sample, the post-reaction measurement of the samples (second sample and third sample) obtained by different dilutions is performed in the first accommodation space. Thus, only one accommodation space for post-reaction measurement is required in the entire measurement process to achieve antigen concentration measurement. Therefore, the method provided by the present application does not require additional components to the existing measuring instrument, and can make full use of the existing measuring instrument to achieve antigen concentration measurement, thereby reducing the cost required for antigen concentration measurement. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] Figure 1 This is a structural diagram of a measuring instrument with a dilution cup added inside the machine;

[0008] Figure 2 This is another structural diagram of the measuring instrument in which a dilution cup is added inside the machine;

[0009] Figure 3 1 is a flow chart of Example 1 of the method for measuring antigen concentration of the present application;

[0010] Figure 4 1 is a flow chart of Example 2 of the antigen concentration measurement method of the present application;

[0011] Figure 5 It is a structural schematic diagram of the measuring instrument of the present application;

[0012] Figure 6 is another structural schematic diagram of the measuring instrument of the present application;

[0013] Figure 7 It is another structural diagram of the measuring instrument of the present application;

[0014] Figure 8 1 is a flow chart of Example 3 of the antigen concentration measurement method of the present application;

[0015] Figure 9 1 is a flow chart of Example 4 of the method for measuring antigen concentration of the present application;

[0016] Figure 10 1 is a flow chart of Example 5 of the antigen concentration measurement method of the present application;

[0017] Figure 11 1 is a flow chart of Example 6 of the method for measuring antigen concentration of the present application;

[0018] Figure 12 1 is a flow chart of a specific example of the antigen concentration measurement method of the present application;

[0019] Figure 13 This is a schematic structural diagram of a first embodiment of the measuring instrument of the present application;

[0020] Figure 14 It is a structural diagram of the second embodiment of the measuring instrument of the present application. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0022] The terms "first," "second," and "third" in this application are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, a feature specified as "first," "second," or "third" may explicitly or implicitly include at least one of the features. In the description of this application, "plurality" means at least two, for example, two, three, etc., unless otherwise specifically specified.

[0023] Reference herein to an "embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor does it refer to independent or alternative embodiments that are mutually exclusive of other embodiments. It is understood, both explicitly and implicitly, by those skilled in the art that the embodiments described herein may be combined with other embodiments unless there is a conflict.

[0024] In many application scenarios, it is necessary to measure the concentration of antigens contained in samples. However, existing detection conditions are not sufficient to support the measurement of antigen concentrations in all numerical ranges. In other words, when the concentration of antigens in a sample exceeds the standard numerical range that can be measured (exceeds the standard), a reagent containing antibodies is added to the sample to allow the antigens in the sample to react with the antibodies; the signal value in the sample after the immune reaction is obtained, and the antigen concentration in the sample obtained by converting the signal value is not the actual concentration.

[0025] Therefore, after diluting the sample, it is necessary to determine whether the antigen concentration in the diluted sample exceeds the standard; if it exceeds the standard, the sample is diluted again to determine whether the antigen in the diluted sample exceeds the standard, and so on, until the antigen concentration in the sample obtained by the last dilution does not exceed the standard; the antigen concentration in the sample obtained by the last dilution is the actual concentration, so the antigen concentration in the original sample can be obtained based on the antigen concentration contained in the sample obtained by the last dilution and the dilution multiple each time.

[0026] Currently, methods for measuring antigen concentration in samples generally use off-machine dilution, pre-reaction, or adding a dilution cup inside the machine.

[0027] The off-site dilution method involves storing the sample off-site, diluting the antigen at a specific ratio, and then measuring the concentration of the diluted sample. This process cannot be fully automated, increasing the workload of the relevant personnel and making antigen concentration measurement highly complex.

[0028] The pre-reaction method requires adding antigen again to each measurement sample. When the antigen concentration exceeds the standard, new antigen is added. The measuring instrument further dilutes the re-injected sample and then performs an excess antigen concentration measurement. This process consumes a lot of reagents, increases the cost of single sample measurement, and is complex to control, placing high demands on the measuring instrument.

[0029] By adding a dilution cup to the machine, the antigen concentration can be automatically measured. Figure 1 and Figure 2 The measurement process of adding a dilution cup to the instrument is explained. The instrument includes multiple dilution cups (taking 1A and 2A as examples) and multiple reaction pools (taking 1B and 2B as examples). 1) Use a sampling needle to add diluent to 1A to dilute the sample in 1A for the Nth time; 2) Use the sampling needle to aspirate part of the sample from 1A back to 1B, and determine in 1B whether the antigen concentration exceeds the standard; if the antigen concentration does not exceed the standard, determine the antigen concentration; if the antigen concentration exceeds the standard, then 3) Use the sampling needle to aspirate part of the sample in 1A back to 2A, and dilute the sample in 2A for the N+1th time; 4) Use the sampling needle to aspirate part of the sample from 2A back to 2B, and determine in 2B whether the antigen concentration exceeds the standard... Therefore, under this method, multiple dilution cups and multiple reaction pools need to be set up in the instrument.

[0030] In summary, the existing methods for measuring antigen concentration are highly complex and costly to implement.

[0031] In order to achieve antigen concentration measurement while reducing cost and complexity, this application proposes an antigen concentration measurement method, which is as follows:

[0032] Figure 3 This is a flow chart of Example 1 of the antigen concentration measurement method of the present application. It should be noted that if there are substantially the same results, this example is not based on Figure 3 The process sequence shown is limited.

[0033] like Figure 3 As shown, this embodiment may include:

[0034] S11: diluting at least a portion of the first sample containing the antigen to obtain a second sample.

[0035] The antigen concentration measurement method is applied to an instrument with antigen concentration measurement function (hereinafter referred to as measuring instrument), such as a specific protein reaction device or an instrument containing a specific protein reaction device. The detailed description of the measuring instrument can be referred to the description of the structural embodiment below.

[0036] The collected first sample of the organism may be placed in advance in a position for storing the first sample in the measuring instrument, and then at least a portion of the first sample may be applied to this step.

[0037] All of the first samples may be diluted, or only part of the first samples may be diluted to reserve the first samples. The reserved first samples may be used in the subsequent step S13.

[0038] The dilution factor of at least a portion of the first sample is known, and thus the volume of diluent required to dilute at least a portion of the first sample can be determined based on the known dilution factor. Thus, diluting at least a portion of the first sample can be performed by adding at least a portion of the first sample and a corresponding volume of diluent to the dilution container.

[0039] The dilution storage space and the reaction storage space (hereinafter referred to as the first storage space) can be the same or different. Specifically, the dilution storage space can be the first storage space, a pipeline connected to the first storage space, or a dedicated dilution cup provided in the measuring instrument. If the dilution storage space is the first storage space, the measuring instrument does not need to include an additional dilution cup, thereby reducing the cost of measuring sample antigen concentration.

[0040] S12: After reacting the second sample in the first containing space, measurement is performed to determine whether the antigen concentration in the second sample exceeds the standard.

[0041] In this step, the post-reaction measurement can be performed on all the second samples, or only on some of the second samples, so as to reserve the second samples. The reserved second samples can be applied to S13.

[0042] If the dilution storage space is the first storage space, reserving the second sample may involve transferring a portion of the second sample from the first storage space to the second storage space. If the dilution storage space is not the first storage space, reserving the second sample may involve leaving a portion of the second sample in the dilution storage space so that the portion of the second sample does not participate in the reaction in the first storage space. The first storage space may be a reaction tank or another storage space available for reaction.

[0043] If the antigen concentration in the second sample exceeds the standard, execute S13-S14; otherwise, execute S15.

[0044] S13: dilute to obtain a third sample containing the antigen.

[0045] For simplicity, the sample that needs to be diluted to obtain the third sample will be referred to as the dilution subject in this application. The dilution subject can be the reserved first sample, the reserved second sample, or another sample. The other sample is obtained by diluting the first sample, and the dilution factor of the first sample to obtain the other sample is known.

[0046] Therefore, in this step, at least another portion of the first sample (the reserved first sample) can be diluted again to obtain a third sample; the second sample (the reserved second sample) can be diluted again to obtain a third sample; or other samples can be diluted to obtain a third sample.

[0047] The dilution factor required to obtain the third sample (the dilution factor for the dilution body) is known. Thus, the volume of diluent required to obtain the third sample can be determined. The reserved first sample, reserved second sample, or other sample can then be added to the dilution chamber along with the determined volume of diluent to obtain the third sample.

[0048] The dilution factor required to obtain the third sample and the dilution factor required to obtain the second sample may be the same or different.

[0049] If they are the same, a fixed dilution factor can be set before the first dilution, and subsequent dilutions of different samples are performed according to this fixed dilution factor. In this way, if each dilution is based on the sample obtained from the previous dilution, then the dilution factor is negatively correlated with the number of dilutions, and the antigen concentration in the original sample (the first sample) is positively correlated with the number of dilutions. After the first dilution, if the antigen concentration does not exceed the standard, the dilution number is 1. If the antigen concentration exceeds the standard after the first dilution, the dilution number is greater than or equal to 2.

[0050] If they are different, the dilution factor corresponding to each dilution can be pre-set, and each dilution can be performed according to the corresponding dilution factor. Alternatively, the dilution factor for the next dilution can be determined based on the exceeding standard result obtained from the previous dilution and reaction measurement, so that the antigen concentration in the sample obtained by the next dilution does not exceed the standard, thereby reducing the number of dilutions and post-reaction measurements.

[0051] S14: Performing post-reaction measurement on the third sample in the first accommodating space.

[0052] The manner of measuring the third sample after the reaction is similar to the manner of measuring the second sample after the reaction, and will not be described in detail here.

[0053] S15: Determine the antigen concentration in the second sample.

[0054] When the antigen concentration in the second sample does not exceed the standard, the antigen concentration in the original sample (first sample) can be obtained by directly multiplying the dilution factor when the second sample is obtained by the antigen concentration in the second sample.

[0055] Through the implementation of this embodiment, the present application performs antigen concentration measurement on the sample, and the post-reaction measurement of the samples (second sample and third sample) obtained by different dilutions is all in the first accommodation space. Thus, only one accommodation space for post-reaction measurement is required during the entire measurement process to achieve antigen concentration measurement. Therefore, the method provided by the present application does not require additional components to the existing measuring instrument, and can make full use of the existing measuring instrument to achieve antigen concentration measurement, thereby reducing the cost required for antigen concentration measurement.

[0056] In addition, when the second sample is reserved before S12, the above embodiment 1 can be further expanded to obtain the following embodiment 2. Parts in this embodiment that are the same as those in the embodiment 1 are not repeated.

[0057] Figure 4 This is a flow chart of Example 2 of the antigen concentration measurement method of this application. It should be noted that if there are substantially the same results, this example is not based on Figure 4 The process sequence shown is limited.

[0058] like Figure 4 As shown, this embodiment may include:

[0059] S21: diluting at least a portion of the first sample in the first accommodating space to obtain a second sample.

[0060] This step defines the accommodating space for dilution as the first accommodating space, so that the dilution and reaction of the first sample are both performed in the first accommodating space.

[0061] A diluent can be added to the first accommodating space via a first transmission component, and a first sample can be added to the first accommodating space via a second transmission component, thereby diluting at least a portion of the first sample. The first transmission component can be a diluent line connected to the first accommodating space, or a sampling needle for collecting the diluent into the first accommodating space. The second transmission component can be a sample line connected to the first accommodating space, or a sampling needle for collecting the first sample into the first accommodating space.

[0062] S22: Move part of the second samples in the first accommodation space into the second accommodation space.

[0063] The second accommodating space may be any accommodating space in the measuring instrument that can be used to store the portion of the second sample.

[0064] However, in order to achieve the second sample without introducing additional components into the measuring instrument, the first transmission assembly can be used as the second accommodation space, the second transmission assembly can be used as the second accommodation space, or the third transmission assembly (see below) can be used as the second accommodation space. Therefore, in this step, part of the second sample in the first accommodation space can be moved into at least one of the first, second, or third transmission assemblies serving as the second accommodation space.

[0065] Before moving the portion of the second sample into the second storage space, it can also be determined whether the second storage space meets the transfer conditions. If so, the portion of the second sample is moved into the second storage space; if not, the portion of the second sample is moved into the third storage space. The transfer conditions may include the second storage space being vacant, cleaned, and connected to the first storage space.

[0066] If there are multiple components in the measuring instrument that can be used as the second storage space, candidate second storage spaces can be displayed on the user interface; a selection instruction is received, and a portion of the second sample is moved into the second storage space indicated by the selection instruction. Specifically, the user can select the second storage space to be moved from the candidate second storage spaces based on requirements (capacity, idle status, etc.), thereby generating a selection instruction, and using the selection instruction to control the movement of the portion of the second sample into the selected second storage space.

[0067] S23: After reacting the second sample in the first containing space, measurement is performed to determine whether the antigen concentration in the second sample exceeds the standard.

[0068] If the antigen concentration in the second sample exceeds the standard, S23-S24 are executed.

[0069] S24: Clear the first accommodation space.

[0070] The liquid in the first accommodating space may be drained first, and then the first accommodating space may be cleaned.

[0071] S25: Move part of the second samples in the second accommodation space into the first accommodation space.

[0072] This step involves moving the reserved portion of the second sample back into the first accommodating space to dilute the portion of the second sample again in the first accommodating space to obtain a third sample, and then reacting the third sample in the first accommodating space before measurement.

[0073] If part of the second sample is moved into the second accommodation space in S22, then this step is to move part of the second sample in the second accommodation space back to the first accommodation space. If part of the second sample is moved into the third accommodation space in S22, then this step is to move part of the second sample in the third accommodation space back to the first accommodation space.

[0074] The second embodiment described above is described below using two examples.

[0075] Example 1: The diluent is stored in the first storage tank a. The first transmission component is the first sampling needle, the first accommodating space is the reaction tank b, and the second accommodating space is the first sampling needle.

[0076] See also Figure 5-6 , 1) Use the first sampling needle to draw part of the diluent from the first storage pool a and add it to the reaction pool b, so that after the first sample is subsequently added to the reaction pool b, the first sample is diluted with the diluent to obtain a second sample; 2) Use the first sampling needle to aspirate part of the second sample and store it, and measure the remaining second sample in the reaction pool b after the reaction; 3) When the antigen concentration exceeds the standard, empty the reaction pool b, and add the second sample stored in the first sampling needle to the reaction pool b again; 4) Use the first sampling needle to draw part of the diluent from the first storage pool a and add it to the reaction pool b to obtain a third sample, and measure the third sample after the reaction in the reaction pool b.

[0077] Example 2: The dilution liquid is stored in the first storage tank a. The first transmission component is the conduit c, the first accommodating space is the reaction tank b, and the second accommodating space is the conduit c.

[0078] See also Figure 7 The first storage reservoir a and the reaction reservoir b are connected by a conduit c. A first valve 1 is provided on the side of the conduit c near the first storage reservoir a, and a second valve 2 is provided on the side near the reaction reservoir b. The first and second valves 1 and 2 are opened, and the diluent in the first storage reservoir a is added to the reaction reservoir b. After a predetermined time (depending on the dilution factor), the first valve 1 is closed. After the first sample is subsequently added to the reaction reservoir b, the first sample in the reaction reservoir b is diluted with the diluent to obtain a second sample. A portion of the second sample in the reaction reservoir b is drawn and stored in the pipeline between the first and second valves 1 and 2 in the conduit c, and the second valve 2 is closed. The remaining second sample in the reaction reservoir b is reacted and measured. If it is determined that the antigen concentration exceeds the standard, the reaction reservoir b is emptied. The second valve 2 is opened to re-add the second sample stored in the pipeline between the first and second valves 1 and 2 to the reaction reservoir b. The second valve 2 is kept open, and the first valve 1 is opened to add the diluent in the first storage reservoir a to the reaction reservoir b. The second sample is diluted with the diluent to obtain a third sample, which is then reacted and measured in the reaction reservoir b.

[0079] As mentioned in the above description of S12, the dilution factor for the next dilution can be determined based on the excess result obtained from the previous dilution and reaction. In this case, whether the antigen concentration in the third sample exceeds the standard is foreseeable, and the above embodiment 1 can be expanded to obtain the following embodiment 3.

[0080] Figure 8 This is a flow chart of Example 3 of the antigen concentration measurement method of this application. It should be noted that if there are substantially the same results, this example is not based on Figure 8 In this embodiment, S31 is a step that can be included before S13, S32 is a further extension of S13, and S33 is a further extension of S14. Figure 8 As shown, this embodiment may include:

[0081] S31: Based on the result that the antigen concentration in the second sample exceeds the standard, determining the dilution factor required to obtain the third sample.

[0082] If the antigen concentration in the second sample exceeds the standard, the extent to which the antigen concentration in the second sample exceeds the standard can be determined based on the measurement results, and then the dilution factor required for the third sample to be obtained in order to ensure that the antigen concentration in the third sample does not exceed the standard can be predicted.

[0083] S32: diluting the sample using a dilution factor to obtain a third sample.

[0084] When the accommodating space for dilution is the first accommodating space, the volume of diluent required to obtain the third sample can be determined based on the dilution multiple and the volume of the dilution body, and the determined volume of diluent and the dilution body are added to the first accommodating space to obtain the third sample.

[0085] Given the limited capacity of the first accommodating space, if the volume of diluent required to obtain the third sample is determined directly based on the dilution factor and the volume of the dilution body, the first accommodating space may be insufficient to accommodate the obtained third sample, resulting in the first accommodating space overflowing. To address this issue, the volume of the dilution body added to the first accommodating space can be limited, thereby limiting the volume of diluent required to dilute the dilution body and, in turn, limiting the volume of the obtained third sample.

[0086] Taking the example of the dilution subject being at least another portion of the first sample or the second sample, the volume of the at least another portion of the first sample or the second sample to be added to the first accommodating space can be determined based on the dilution factor without causing the first accommodating space to overflow; the at least another portion of the first sample or the second sample is added to the first accommodating space according to the determined volume, and the diluent is added to the first accommodating space to obtain a third sample.

[0087] S33: Measure all third samples after reaction in the first accommodating space.

[0088] When the third sample is obtained by dilution using the dilution factor determined in S31, the antigen concentration in the third sample does not exceed the standard. Therefore, there is no need to reserve the third sample for the next dilution. Furthermore, this step can be used to perform post-reaction measurement on all third samples.

[0089] Through the implementation of this embodiment, since the dilution factor can be determined based on the result that the antigen concentration of the second sample exceeds the standard, and the antigen concentration in the third sample obtained by diluting the second sample according to the determined dilution factor does not exceed the standard, the antigen concentration contained in the third sample can be directly determined, and then the concentration of the original sample can be determined, without the need for repeated cyclic dilution, reaction and measurement, thereby saving the cost required for antigen concentration measurement and reducing the complexity of the measurement.

[0090] If the dilution factor for the next dilution is not determined based on the exceeding standard result obtained by the measurement after the previous dilution and reaction, in this case, whether the antigen concentration in the third sample exceeds the standard is unpredictable. The above embodiment can be further expanded to obtain the following embodiment 4.

[0091] Figure 9 This is a flow chart of Example 4 of the antigen concentration measurement method of the present application. It should be noted that if there are substantially the same results, this example is not based on Figure 9 The process sequence shown is limited. In this embodiment, S41 is a step that can be included before S14, and S42 is a further extension of S14. Figure 9 As shown, this embodiment may include:

[0092] S41: moving part of the third sample into the second accommodating space.

[0093] Considering that it is unknown whether the antigen concentration of the third sample exceeds the standard, before reacting the third sample in the first accommodation space, part of the third sample can be moved into the second accommodation space to reserve the third sample. The reserved third sample can be used for the next dilution.

[0094] If the second sample has been reserved in the second accommodating space before, the second accommodating space may be cleaned before part of the third sample is moved into the second accommodating space.

[0095] S42: performing post-reaction measurement in the first accommodation space on the third sample remaining after being moved into the second accommodation space.

[0096] In addition, after S14, it is also possible to determine whether the antigen concentration in the third sample exceeds the standard based on the measurement result after the reaction of the third sample, so the above embodiment 1 can be expanded into the following embodiment 5.

[0097] Figure 10This is a flow chart of Example 5 of the antigen concentration measurement method of the present application. It should be noted that if there are substantially the same results, this example is not based on Figure 10 The process sequence shown is limited. In this embodiment, S41 is a step that can be included before S14, and S42 is a further extension of S14. Figure 10 As shown, this embodiment may include:

[0098] S51: Determine whether the antigen concentration in the third sample exceeds the standard.

[0099] If the antigen concentration in the third sample exceeds the standard, execute S52; otherwise, execute S53.

[0100] S52: diluting to obtain a fourth sample containing the antigen, and using the fourth sample to replace the third sample.

[0101] After this step is executed, the process jumps to S51 and repeats the above steps until the antigen concentration detected for the last time does not exceed the standard.

[0102] S53: Determine the antigen concentration in the third sample.

[0103] For other detailed descriptions of this embodiment, please refer to other embodiments and will not be repeated here.

[0104] In addition, the above-mentioned process of performing post-reaction measurement on the second sample and the third sample to determine whether the antigen concentration therein exceeds the standard is similar. The following Example 6 describes the post-reaction measurement process in detail using the second sample as an example.

[0105] Figure 11 This is a flow chart of Example 6 of the antigen concentration measurement method of this application. It should be noted that if there are substantially the same results, this example is not based on Figure 11 The process sequence shown is limited. Figure 11 As shown, this embodiment may include:

[0106] S61: Adding a reagent containing an antibody into the first containing space via the third transmission component, so that the antigen contained in the second sample reacts with the antibody for immune reaction.

[0107] The third transmission component may be a reagent pipeline connected to the first accommodating space, or may be a reagent needle for collecting a reagent containing an antibody.

[0108] S62: Perform antigen detection on the second sample after the immune response to determine whether the antigen concentration exceeds the standard.

[0109] A signal value corresponding to the second sample after the immune reaction can be collected; based on the signal value, it is determined whether the antigen concentration exceeds the standard. The signal value can be an optical signal value or an electrical signal value.

[0110] It is understood that the method for determining whether the antigen in the second sample exceeds the standard is immune turbidimetry. The so-called immune turbidimetry refers to the phenomenon that the antigen and antibody combine to produce a specific reaction (immune reaction) and form an insoluble complex. According to the Heidelberg curve, when the antibody exceeds the standard (the antigen does not exceed the standard), the concentration of the insoluble complex formed is directly proportional to the antigen concentration, and the signal intensity is directly proportional to the concentration of the insoluble complex, thereby obtaining a direct proportional relationship between the signal intensity and the antigen concentration. Therefore, the signal intensity can be used to determine whether the antigen exceeds the standard.

[0111] The following combination Figure 12 , the method provided in this application is described in the form of a specific example.

[0112] Example 3:

[0113] 1) Adding a sample containing an antigen (original sample) to a reaction pool, and performing an initial dilution treatment on the sample in the reaction pool to obtain a primary diluted sample;

[0114] 2) transferring part of the primary dilution sample to another unit (diluent line) outside the reaction pool, and performing an immune reaction on the remaining primary dilution sample in the reaction pool to determine whether the antigen concentration in the primary dilution sample is excessive / exceeds the standard; if the antigen concentration in the primary dilution sample is excessive, proceed to 3); if the antigen concentration in the primary dilution sample is not excessive, determine the antigen concentration;

[0115] 3) adding the primary dilution sample in the dilution liquid pipeline to the reaction cell and diluting it to obtain a secondary dilution sample;

[0116] 4) transferring part of the secondary diluted sample to other units outside the reaction pool, and performing an immune reaction on the remaining secondary diluted sample in the reaction pool to determine whether the antigen concentration in the secondary diluted sample is excessive.

[0117] Figure 13 This is a schematic diagram of the structure of the measuring instrument embodiment 1 of the present application. Figure 13 As shown, the measuring instrument 1 is used to measure the antigen concentration of a sample. Figure 12 As shown, the measuring instrument 1 may include a power component 11 , a measuring component 12 and a control circuit 13 , and is provided with a first accommodating space 14 and a second accommodating space 15 . The control circuit 13 connects the power component 11 and the measuring component 12 .

[0118] The control circuit 13 can be used to control the power component 11 to dilute the first sample containing the antigen to obtain the second sample; after the second sample reacts in the first accommodating space, the measurement component 12 is controlled to measure to determine whether the antigen concentration in the second sample exceeds the standard.

[0119] If the antigen concentration in the second sample exceeds the standard, the measurement result of the measuring component 12 is the antigen concentration in the second sample. If the antigen concentration in the second sample exceeds the standard, the control circuit 13 is also used to control the power component 11 to dilute and obtain a third sample containing the antigen; after the first accommodating space 14 reacts with the third sample, the measuring component 12 is controlled to perform measurement. Among them, the dilution subject when obtaining the third sample can be at least another part of the first sample or the second sample, or other samples. In the case where the dilution subject is at least another part of the first sample or the second sample, the control circuit 13 can also be used to control the power component 11 to dilute at least another part of the first sample or the second sample again to obtain the third sample.

[0120] Furthermore, the aforementioned sample dilution and post-reaction measurement can both be performed in the first accommodating space 14. In this case, the control circuit 13 can be used to control the power assembly 11 to dilute the first sample in the first accommodating space to obtain the second sample; and after the first accommodating space 14 reacts with the second sample but before controlling the measurement assembly 12 to perform measurement, control the power assembly 11 to move a portion of the second sample from the first accommodating space 14 into the second accommodating space 15.

[0121] Figure 14 This is a schematic diagram of the structure of the second embodiment of the measuring instrument of this application. Figure 14 As shown, based on the above embodiment 1, the measuring instrument 1 can further include a first transmission component 151, a second transmission component 151 and a third transmission component 153. The first transmission component 151, the second transmission component 151 and the third transmission component 153 can all be used as the second accommodating space 15.

[0122] The first transmission component 151 can be used to transmit the diluent, and the second transmission component 152 can be used to transmit the first sample. The control circuit 13 can be used to control the power component 11 to add the diluent to the first accommodating space 14 via the first transmission component 151, and to add the first sample to the first accommodating space 14 via the second transmission component 152.

[0123] The third transmission component 153 can be used to transport a reagent containing an antibody. The control circuit 13 can be used to control the power component 11 to add the antibody-containing reagent to the first accommodating space 14 via the third transmission component 153, so that the antigen contained in the second sample reacts with the antibody; and control the measurement component 12 to perform antigen detection on the second sample after the immune reaction to determine whether the antigen concentration exceeds the standard.

[0124] In the structural embodiment of the measuring instrument 1 of this application, please refer to the previous method embodiment for any details not discussed in detail.

[0125] In the several embodiments provided in this application, it should be understood that the disclosed systems, devices and methods can be implemented in other ways. For example, the device embodiments described above are merely schematic. For example, the division of units is only a logical function division. In actual implementation, there may be other division methods, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be an indirect coupling or communication connection through some interface, device or unit, which can be electrical, mechanical or other forms.

[0126] In addition, each functional unit in each embodiment of the present application can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit. The above-mentioned integrated unit can be implemented in the form of hardware or in the form of a software functional unit. The above is only an implementation method of the present application and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the content of the description and drawings of this application, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A measuring instrument, characterized in that: The device comprises a power component, a measuring component and a control circuit, and is provided with a first accommodation space and a second accommodation space, wherein the control circuit connects the power component and the measuring component; wherein, the control circuit is used to control the power component to dilute the first sample containing the antigen in the first accommodation space to obtain the second sample, and to control the power component to move part of the second sample in the first accommodation space into the second accommodation space; wherein, the second accommodation space is at least one of a first transmission component for transmitting a diluent, a second transmission component for transmitting the first sample, and a third transmission component for transmitting a reagent containing an antibody; the first transmission component is a diluent pipeline connected to the first accommodation space or a sampling needle for collecting the diluent into the first accommodation space, the second transmission component is a sample pipeline connected to the first accommodation space or a sampling needle for collecting the first sample into the first accommodation space, and the third transmission component is a reagent pipeline connected to the first accommodation space or a reagent needle for collecting a reagent containing an antibody; after the second sample reacts in the first accommodation space, the measuring component is controlled to perform measurement to determine whether the antigen concentration in the second sample exceeds the standard; If the antigen concentration in the second sample exceeds the standard, the control circuit is used to control the power component to dilute the portion of the second sample moved into the second accommodation space in the first accommodation space to obtain a third sample containing the antigen; and control the measurement component to perform measurement after the third sample reacts in the first accommodation space.

2. The apparatus according to claim 1, wherein The control circuit is used to control the power component to dilute at least another portion of the first sample or the second sample again to obtain the third sample.

3. The apparatus according to claim 1, wherein The measuring instrument includes a first transmission component and a second transmission component; The control circuit is specifically used to control the power component to add the diluent into the first accommodation space via the first transmission component, and to add the first sample into the first accommodation space via the second transmission component; the first transmission component or the second transmission component is used as the second accommodation space; or The measuring instrument includes a third transmission assembly serving as the second accommodation space; Among them, the control circuit is specifically used to control the power component to add the antibody-containing reagent into the first accommodating space via the third transmission component, so that the antigen contained in the second sample reacts immune-wise with the antibody; and control the measuring component to perform antigen detection on the second sample after the immune reaction to determine whether the antigen concentration exceeds the standard.

4. The apparatus according to claim 1, wherein The measuring instrument is further used to empty the first accommodating space; and move the portion of the second sample in the second accommodating space into the first accommodating space.

5. The apparatus according to claim 1, wherein The power component is further used to move part of the second sample in the first accommodation space into the third transmission component serving as the second accommodation space.

6. The apparatus according to claim 1, wherein: The measuring instrument is further used to determine whether the second accommodation space meets the moving-in condition; If the conditions are met, moving the portion of the second sample into the second accommodating space; If not, the portion of the second sample is moved into the third accommodating space.

7. The apparatus according to claim 1, wherein: The measuring instrument is further configured to display the candidate second accommodation space on a user interface; receive a selection instruction, and move the portion of the second samples into the second accommodation space indicated by the selection instruction.

8. The apparatus according to claim 1, wherein: The measuring instrument is also used to determine the dilution factor required to obtain the third sample based on the result that the antigen concentration in the second sample exceeds the standard; dilute the third sample using the dilution factor; and control the measuring device to perform measurement after all the third samples are reacted in the first accommodating space.

9. The apparatus according to claim 8, wherein The power assembly is further configured to add at least another portion of the first sample or the second sample into the first accommodating space, and to add a diluent into the first accommodating space; The measuring instrument is further configured to determine a volume of the at least another portion of the first sample or the second sample to be added to the first accommodating space based on the dilution factor without causing the first accommodating space to overflow.

10. The apparatus according to claim 1, wherein The power component is further used to move part of the third sample into the second accommodating space; The control circuit is further configured to control the measuring component to perform measurement after the first accommodating space reacts to the third sample remaining after the third sample is moved into the second accommodating space.

11. The apparatus according to claim 1, wherein The measuring instrument is used to determine whether the antigen concentration in the third sample exceeds the standard; if the antigen concentration in the third sample does not exceed the standard, then determine the antigen concentration in the third sample.

12. The apparatus according to claim 11, wherein If the measuring instrument determines that the antigen concentration in the third sample exceeds the standard, diluting the third sample to obtain a fourth sample containing the antigen; The fourth sample is used to replace the third sample, and the process jumps to the step of determining whether the antigen concentration in the third sample exceeds the standard, until the antigen concentration detected for the last time does not exceed the standard.

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