High-throughput microscope objective

Abstract

This invention discloses a high-throughput microscope objective, belonging to the field of microscopy technology. It comprises a first lens, a second lens, a first lens group, a second lens group, a third lens group, a fourth lens group, a twelfth lens, and a fifth lens group, arranged coaxially from the object side to the image side. Through this design, while ensuring sufficient resolution, the single-image field of view of the microscope objective is increased from the original FN26 to at least FN32. Without considering image stitching and overlap, the number of images required is reduced from 100 to 64 or even less, thus improving the efficiency of rapid pathological slide reading to a certain extent. Furthermore, it achieves apochromatic correction in the 480nm to 644nm visible light band, effectively correcting spherical aberration, coma, astigmatism, field curvature, distortion, axial chromatic aberration, and transverse chromatic aberration, meeting the requirements of a plan-field apochromatic objective.

Description

Technical Field

[0001] This invention relates to the field of microscopy, and more particularly to high-throughput microscope objectives. Background Technology

[0002] Cervical cancer has seen a surge in incidence in recent years, ranking fourth in both morbidity and mortality among malignant tumors affecting women worldwide. Currently, the most effective diagnostic method for cervical cancer is cervical imaging pathology. This method requires rapid, high-resolution scanning to convert traditional glass slides into digital slides, which are then examined under a microscope by a pathologist to identify diseased cells and make a diagnosis. Modern rapid pathology slide reading utilizes wide-field microscopic imaging technology. A high-precision stage is used to capture and stitch together multiple images of the cervix, combined with intelligent algorithms to automate lesion diagnosis. This not only replaces the manual interpretation process by pathologists but also significantly accelerates cervical cancer diagnosis, achieving high throughput in rapid pathology slide reading.

[0003] However, the cervical images required for biological diagnosis of cervical cancer are currently quite large (approximately 13mm in diameter), and the numerical aperture of the microscope objective lens required for observing and resolving cervical lesions must be at least 0.75. Traditional microscope objectives, with a numerical aperture of at least 0.75, only have a maximum field of view of approximately FN26 per image. This means that stitching together a complete cervical image requires at least 100 images (and even more if overlapping areas are considered for perfect stitching without overlap), significantly impacting the overall efficiency of rapid pathological examination. Improving stitching speed comes at the cost of sacrificing the numerical aperture of the microscope objective lens, resulting in insufficient resolution.

[0004] Therefore, there is an urgent need for a high-throughput microscope objective that can balance a large field of view and high resolution (i.e., high numerical aperture) in rapid pathological slide reading technology. Summary of the Invention

[0005] In order to overcome the shortcomings of the prior art, one of the objectives of this invention is to provide a high-throughput microscope objective that can take into account both a large field of view and high resolution (i.e., high numerical aperture).

[0006] One of the objectives of this invention is achieved through the following technical solution:

[0007] A high-throughput microscope objective includes a first lens, a second lens, a first lens group, a second lens group, a third lens group, a fourth lens group, a twelfth lens, and a fifth lens group arranged coaxially from the object side to the image side. The first lens has a concave surface facing the object side and a convex surface facing the image side. The second lens has a concave surface facing the object side and a convex surface facing the image side. The twelfth lens has a concave surface facing the object side and a convex surface facing the image side. The first lens group, the second lens group, the third lens group, the fourth lens group, and the fifth lens group each include at least two lenses with different refractive indices. The first lens group has a convex surface facing both the object and image sides. The second lens group has a convex surface facing both the object and image sides. The third lens group has a convex surface facing both the object and image sides. The fifth lens group has a concave surface facing both the object and image sides.

[0008] Furthermore, the first lens is a Qiming lens, used to increase the object-side field of view when the numerical aperture is high.

[0009] Furthermore, the second lens is a positive meniscus lens, used to handle the high optical power required for high numerical aperture and to adjust coma.

[0010] Furthermore, the first lens group includes a third lens, a fourth lens, and a fifth lens, which are sequentially glued and fixed together.

[0011] Furthermore, the third lens has a convex surface facing both the object and image sides, the fourth lens has a concave surface facing both the object and image sides, and the fifth lens has a convex surface facing both the object and image sides.

[0012] Furthermore, the second lens group includes a sixth lens and a seventh lens, which are glued together and fixed in sequence.

[0013] Furthermore, the sixth lens has a convex surface facing the object and a concave surface facing the image, and the seventh lens has a convex surface facing both the object and image.

[0014] Furthermore, the third lens group includes an eighth lens and a ninth lens, which are sequentially glued and fixed together.

[0015] Furthermore, the eighth lens has a convex surface facing the object and a concave surface facing the image, and the ninth lens has a convex surface facing both the object and image.

[0016] Furthermore, the fourth lens group includes a tenth lens and an eleventh lens, which are cemented together and fixed.

[0017] Furthermore, the tenth lens has a convex surface facing the object and a concave surface facing the image, and the eleventh lens has a convex surface facing the object and a concave surface facing the image.

[0018] Furthermore, the twelfth lens is a double meniscus lens, used for compensating for various aberrations.

[0019] Furthermore, the fifth lens group includes a thirteenth lens and a fourteenth lens, which are cemented together and fixed.

[0020] Furthermore, the thirteenth lens has a concave surface facing both the object and image sides, while the fourteenth lens has a convex surface facing both the object and image sides.

[0021] Compared to existing technologies, this invention's high-throughput microscope objective, while ensuring sufficient resolution, increases the number of fields of view per imaging from the original FN26 to at least FN32. Without considering image stitching and overlap, it reduces the number of images required from 100 to 64 or even less, thus improving the efficiency of rapid pathological slide review. Furthermore, it achieves apochromatic correction in the 480nm to 644nm visible light band, effectively correcting spherical aberration, coma, astigmatism, field curvature, distortion, axial chromatic aberration, and transverse chromatic aberration, meeting the requirements of a plan-field apochromatic objective. Attached Figure Description

[0022] Figure 1 This is a structural diagram of the high-throughput microscope objective lens of the present invention;

[0023] Figure 2 for Figure 1 Optical path diagram of a high-throughput microscope objective;

[0024] Figure 3 for Figure 1 Lateral aberration map of 0.0 field of view of a high-throughput microscope objective;

[0025] Figure 4 for Figure 1 Lateral aberration map of the 0.5 field of view of a high-throughput microscope objective;

[0026] Figure 5 for Figure 1 Lateral aberration map of a high-throughput microscope objective lens at 0.7 field of view;

[0027] Figure 6 for Figure 1 Lateral aberration map of a 1.0 field of view objective lens for a high-throughput microscope;

[0028] Figure 7 for Figure 1The full-field transfer function curve of a high-throughput microscope objective;

[0029] Figure 8 for Figure 1 Axial chromatic aberration diagram of a high-throughput microscope objective lens;

[0030] Figure 9 for Figure 1 A chromatic aberration diagram of the transverse axis of a high-throughput microscope objective;

[0031] Figure 10 for Figure 1 Field curvature distortion diagram of a high-throughput microscope objective;

[0032] Figure 11 for Figure 1 Strell rate plot of the entire field of view of a high-throughput microscope objective.

[0033] In the diagram: 10, first lens; 20, second lens; 30, first lens group; 31, third lens; 32, fourth lens; 33, fifth lens; 40, second lens group; 41, sixth lens; 42, seventh lens; 50, third lens group; 51, eighth lens; 52, ninth lens; 60, fourth lens group; 61, tenth lens; 62, eleventh lens; 70, twelfth lens; 80, fifth lens group; 81, thirteenth lens; 82, fourteenth lens. Detailed Implementation

[0034] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] It should be noted that when a component is said to be "fixed to" another component, it can be directly on the other component or it can be fixed through another intermediate component. When a component is said to be "connected to" another component, it can be directly connected to the other component or it may be fixed through another intermediate component. When a component is said to be "set on" another component, it can be set directly on the other component or it may be set through another intermediate component. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0037] like Figure 1 As shown, the high-throughput microscope objective lens of the present invention includes a first lens 10, a second lens 20, a first lens group 30, a second lens group 40, a third lens group 50, a fourth lens group 60, a twelfth lens 70, and a fifth lens group 80. The first lens 10, the second lens 20, the first lens group 30, the second lens group 40, the third lens group 50, the fourth lens group 60, the twelfth lens 70, and the fifth lens group 80 are arranged coaxially from the object side to the image side.

[0038] The first lens 10 has a concave surface facing the object side and a convex surface facing the image side; the first lens 10 is configured as a uniform lens to increase the object-side field of view when the numerical aperture is high.

[0039] The second lens 20 has a concave surface facing the object side and a convex surface facing the image side; the second lens 20 is configured as a positive meniscus lens to handle the high optical power required for high numerical aperture and to adjust coma.

[0040] The first lens group 30 includes a third lens 31, a fourth lens 32, and a fifth lens 33. The third lens 31, fourth lens 32, and fifth lens 33 are arranged sequentially from the object side to the image side. The third lens 31 has a convex surface facing both the object and image sides; the fourth lens 32 has a concave surface facing both the object and image sides; and the fifth lens 33 has a convex surface facing both the object and image sides. The convex surface of the third lens 31 facing the image side and the concave surface of the fourth lens 32 facing the object side are cemented together; the concave surface of the fourth lens 32 facing the image side and the convex surface of the fifth lens 33 facing the object side are cemented together. The cemented third lens 31, fourth lens 32, and fifth lens 33 form the first lens group 30. The first lens group 30 produces negative spherical aberration, negative coma, positive astigmatism, positive field curvature, positive distortion, negative transverse chromatic aberration, and positive magnification chromatic aberration to correct for second-order chromatic aberration.

[0041] The second lens group 40 includes a sixth lens 41 and a seventh lens 42, which are cemented together sequentially from the object side to the image side. The sixth lens 41 has a convex surface facing the object side and a concave surface facing the image side, while the seventh lens 42 has a convex surface facing both the object and image sides. The concave surface of the sixth lens 41 facing the image side and the convex surface of the seventh lens 42 facing the object side are cemented together to form the second lens group 40. The second lens group 40 produces negative spherical aberration, negative coma, positive astigmatism, positive field curvature, positive distortion, negative transverse chromatic aberration, and positive magnification chromatic aberration to correct for second-order chromatic aberration.

[0042] The third lens group 50 includes an eighth lens 51 and a ninth lens 52, which are cemented together sequentially. The eighth lens 51 has a convex surface facing the object side and a concave surface facing the image side, while the ninth lens 52 has a convex surface facing both the object and image sides. The concave surface of the eighth lens 51 facing the image side and the convex surface of the ninth lens 52 facing the object side are cemented together to form the third lens group 50. The third lens group 50 produces negative spherical aberration, negative coma, positive astigmatism, positive field curvature, positive distortion, negative transverse chromatic aberration, and positive magnification chromatic aberration to correct for second-order chromatic aberration.

[0043] The fourth lens group 60 includes a tenth lens 61 and an eleventh lens 62, which are cemented together sequentially. The tenth lens 61 has a convex surface facing the object side and a concave surface facing the image side; the eleventh lens 62 has a convex surface facing the object side and a concave surface facing the image side. The concave surface of the tenth lens 61 facing the image side and the convex surface of the eleventh lens 62 facing the object side are cemented together to form the fourth lens group 60. The fourth lens group 60 produces negative spherical aberration, negative coma, positive astigmatism, positive field curvature, positive distortion, negative transverse chromatic aberration, and positive magnification chromatic aberration to correct for second-order chromatic aberration.

[0044] The twelfth lens 70 has a concave surface facing the object and a convex surface facing the image; the twelfth lens 70 is configured as a double meniscus lens for compensating for various aberrations.

[0045] The fifth lens group 80 includes a thirteenth lens 81 and a fourteenth lens 82, which are cemented together. The thirteenth lens 81 has a concave surface facing both the object and image sides, while the fourteenth lens 82 has a convex surface facing both the object and image sides. The concave surface of the thirteenth lens 81 facing the image side and the convex surface of the fourteenth lens 82 facing the object side are cemented together.

[0046] The concave surface of the first lens 10 facing the object side is the first mirror surface; the convex surface of the first lens 10 facing the image side is the second mirror surface; the concave surface of the second lens 20 facing the object side is the third mirror surface; the convex surface of the second lens 20 facing the image side is the fourth mirror surface; the convex surface of the third lens 31 facing the object side is the fifth mirror surface; the cemented surface of the third lens 31 and the fourth lens 32 is the sixth mirror surface; the cemented surface of the fourth lens 32 and the fifth lens 33 is the seventh mirror surface; the convex surface of the fifth lens 33 facing the image side is the eighth mirror surface; the convex surface of the sixth lens 41 facing the object side is the ninth mirror surface; the cemented surface of the sixth lens 41 and the seventh lens 42 is the tenth mirror surface; the convex surface of the seventh lens 42 facing the image side is the eleventh mirror surface; and the eighth lens 51 facing the object side... The convex surface of the tenth lens 61 is the twelfth mirror surface; the cemented surface of the eighth lens 51 and the ninth lens 52 is the thirteenth mirror surface; the convex surface of the ninth lens 52 facing the image side is the fourteenth mirror surface; the convex surface of the tenth lens 61 facing the object side is the fifteenth mirror surface; the cemented surface of the tenth lens 61 and the eleventh lens 62 is the sixteenth mirror surface; the concave surface of the eleventh lens 62 facing the image side is the seventeenth mirror surface; the concave surface of the twelfth lens 70 facing the object side is the eighteenth mirror surface; the convex surface of the twelfth lens 70 facing the image side is the nineteenth mirror surface; the concave surface of the thirteenth lens 81 facing the object side is the twentieth mirror surface; the cemented surface of the thirteenth lens 81 and the fourteenth lens 82 is the twenty-first mirror surface; and the convex surface of the fourteenth lens 82 facing the image side is the twenty-second mirror surface.

[0047] Please refer to Table 1 for the structural parameters of the above twenty-two mirrors, where R1 is the radius of curvature of the first mirror; D1 is the mirror distance of the first mirror; ψ1 is the effective aperture of the first mirror; the meanings of R2~R22, D2~D22 and ψ2~ψ22 are deduced accordingly; and Nd / Vd represents the refractive index / Abbe coefficient.

[0048] Table 1 - Structural parameter ranges for twenty-two mirror surfaces

[0049]

[0050]

[0051] In some preferred embodiments, the focal length of the first lens 10 is 25.25 mm, the focal length of the second lens 20 is 29.70 mm, the focal length of the first lens group 30 formed by the combination of the third lens 31, the fourth lens 32, and the fifth lens 33 is 66.66 mm, and the focal length of the second lens group 40 formed by the combination of the sixth lens 41 and the seventh lens 42 is 39.42 mm. The focal length of the third lens group 50 formed by the combination of the eighth lens 51 and the ninth lens 52 is 57.77 mm. The focal length of the fourth lens group 60 formed by the combination of the tenth lens 61 and the eleventh lens 62 is -25.37 mm, the focal length of the twelfth lens 70 is -15.50 mm, and the focal length of the fifth lens group 80 formed by the combination of the thirteenth lens 81 and the fourteenth lens 82 is 35.69 mm.

[0052] In some preferred embodiments, the refractive index / Abbe coefficient of the first lens 10 is 1.88 / 39.2, the refractive index / Abbe coefficient of the second lens 20 is 1.60 / 80.8, the refractive index / Abbe coefficient of the third lens 31 is 1.46 / 90.3, the refractive index / Abbe coefficient of the fourth lens 32 is 1.61 / 44.1, the refractive index / Abbe coefficient of the fifth lens 33 is 1.43 / 95.0, the refractive index / Abbe coefficient of the sixth lens 41 is 1.61 / 44.1, and the refractive index / Abbe coefficient of the seventh lens 42 is 1.43 / 95.0. 5.0, the refractive index / Abbe coefficient of the eighth lens 51 is 1.61 / 44.1, the refractive index / Abbe coefficient of the ninth lens 52 is 1.43 / 95.0, the refractive index / Abbe coefficient of the tenth lens 61 is 1.61 / 58.6, the refractive index / Abbe coefficient of the eleventh lens 62 is 2.00 / 20.7, the refractive index / Abbe coefficient of the twelfth lens 70 is 1.59 / 61.2, the refractive index / Abbe coefficient of the thirteenth lens 81 is 1.67 / 32.2, and the refractive index / Abbe coefficient of the fourteenth lens 82 is 1.75 / 35.0.

[0053] Please continue reading. Figure 2 , Figure 2 The optical path diagram of the high-throughput microscope objective of this invention is shown below. The high-throughput microscope objective satisfies the following conditions: 0.75 ≤ NA < 1, where NA represents the numerical aperture of the microscope objective. The microscope objective satisfies the following conditions: 32 ≤ FN < 40, where FN represents the field of view number of the microscope objective. The microscope objective satisfies the following conditions: 0.7 ≤ WD < 1.2, where WD represents the distance from the rear surface of the coverslip away from the object side to the front surface of the first lens 10 on the object side.

[0054] Please continue reading. Figure 3 , Figure 3 The lateral aberration diagram of the microscope objective lens at 0.0 field of view provided in the embodiment of the present invention. Figure 3 The horizontal axes PY and PX represent the entrance pupil, and the vertical axes EY and EX represent the lateral aberrations. Y represents the meridional direction and X represents the sagittal direction. Different wavelengths of light are indicated by different colors. The lateral aberrations at each wavelength and entrance pupil position in the band from 0.480μm to 0.644μm are all within ±1μm. As can be seen from the figure, the aberration balance is very good, and the imaging is excellent. Figure 3 The horizontal axis represents the normalized entrance pupil; the vertical axis ranges from a maximum of +1 μm to a minimum of -1 μm.

[0055] Please continue reading. Figure 4 , Figure 4 Lateral aberration diagram of a microscope objective lens at 0.5 field of view provided in an embodiment of the present invention. Figure 4The horizontal axes PY and PX represent the entrance pupil, and the vertical axes EY and EX represent the lateral aberrations. Y represents the meridional direction and X represents the sagittal direction. Different wavelengths of light are indicated by different colors. The lateral aberrations at each wavelength and entrance pupil position in the band from 0.480μm to 0.644μm are all within ±2μm. As can be seen from the figure, the aberration balance is very good, and the imaging is excellent. Figure 4 The horizontal axis represents the normalized entrance pupil; the vertical axis has a maximum value of +2μm and a minimum value of -2μm.

[0056] Please continue reading. Figure 5 , Figure 5 Lateral aberration diagram of a microscope objective lens with a field of view of 0.7 provided in an embodiment of the present invention. Figure 5 The horizontal axes PY and PX represent the entrance pupil, and the vertical axes EY and EX represent the lateral aberrations. Y represents the meridional direction and X represents the sagittal direction. Different wavelengths of light are indicated by different colors. The lateral aberrations at each wavelength and entrance pupil position in the band from 0.480μm to 0.644μm are all within ±2μm. As can be seen from the figure, the aberration balance is very good, and the imaging is excellent. Figure 5 The horizontal axis represents the normalized entrance pupil; the vertical axis has a maximum value of +2μm and a minimum value of -2μm.

[0057] Please continue reading. Figure 6 , Figure 6 Lateral aberration diagram of a microscope objective lens with a field of view of 1.0 provided in an embodiment of the present invention. Figure 6 The horizontal axes PY and PX represent the entrance pupil, and the vertical axes EY and EX represent the lateral aberrations. Y represents the meridional direction and X represents the sagittal direction. Different wavelengths of light are indicated by different colors. The lateral aberrations at each wavelength and entrance pupil position in the band from 0.480μm to 0.644μm are all within ±4μm. As can be seen from the figure, the aberration balance is very good, and the imaging is excellent. Figure 6 The horizontal axis represents the normalized entrance pupil; the vertical axis has a maximum value of +5μm and a minimum value of -5μm.

[0058] Please continue reading. Figure 7 , Figure 7 The full-field transfer function curve of the microscope objective provided in the embodiment of the present invention. Figure 7 The vertical axis represents the normalized OTF modulus, and the horizontal axis represents the spatial frequency, with units of lp / mm. The figure shows that both the on-axis and off-axis field-of-view transfer function curves are close to the diffraction limit, indicating that the optical system has high imaging contrast across the entire field of view and distinct image detail.

[0059] Please continue reading. Figure 8 , Figure 8 This is an axial chromatic aberration diagram of a microscope objective lens provided in an embodiment of the present invention. Figure 8The vertical axis represents the entrance pupil, and the horizontal axis represents the longitudinal aberration, both in mm. Different wavelengths are indicated by different colors. The axial chromatic aberration at any entrance pupil position for any two wavelengths within the 0.480μm to 0.644μm band is less than 2λ / NA², reaching apochromatic levels. The vertical axis in the figure represents the normalized entrance pupil; the horizontal axis represents the longitudinal aberration, with a maximum of 0.0001mm and a minimum of -0.0001mm.

[0060] Please continue reading. Figure 9 , Figure 9 A transverse chromatic aberration diagram of a microscope objective lens provided in an embodiment of the present invention. Figure 9 The vertical axis represents the field of view, and the horizontal axis represents the lateral aberration, with units of μm. The dashed line indicates the size of the system's diffraction-limited Airy disk, and the solid line indicates that the transverse chromatic aberration in the 0.480 μm to 0.644 μm band is within the Airy disk range of the entire field of view.

[0061] Please continue reading. Figure 10 , Figure 10 Field curvature distortion diagram of a microscope objective lens provided in an embodiment of the present invention. Figure 10 The left-middle image is the field curvature diagram. The vertical axis represents the field of view, and the horizontal axis represents the field curvature, both in μm. Different wavelengths are indicated by different colors. The field curvature values ​​for each wavelength and position within the field of view, from 0.480 μm to 0.644 μm, are all within ±1 μm. The axial difference between the optimal focusing point at the edge of the field of view and the optimal focusing point at the center of the field of view is less than 2λ / NA². Theoretically, this satisfies the requirement for sharpness across the entire field of view, meeting the requirements for a field-plan objective. The vertical axis in the diagram represents the normalized field of view; the horizontal axis represents the field curvature, with a maximum of 2 μm and a minimum of -2 μm. Figure 10 The right-hand side of the figure shows the distortion diagram. The vertical axis represents the field of view, and the horizontal axis represents the distortion (percentage). Different wavelengths are indicated by different colors. The distortion at each wavelength and position within the field of view from 0.480μm to 0.644μm is less than 1.51%. The vertical axis represents the normalized field of view; the horizontal axis represents the distortion, with a maximum of 2.0% and a minimum of -2.0%.

[0062] Please continue reading. Figure 11 , Figure 11 This is a full-field Strellier ratio diagram of a microscope objective provided in an embodiment of the present invention. The vertical axis represents the Strellier ratio value, and the horizontal axis represents the field of view. (Strillier ratio: the ratio of the peak intensity in the diffraction pattern of an aberrated point image to the peak intensity in the diffraction pattern of an aberrated point image. A high Strellier ratio indicates a high level of aberration correction.) A Strellier ratio of 0.8 represents the diffraction limit. Different colors are used to indicate different imaging fields of view. From 0.480 μm to 0.644 μm, the Strellier ratio is greater than the diffraction limit in all fields of view, approximately equal to or greater than 0.9.

[0063] The microscope objective provided by this invention, while maintaining high numerical aperture and high resolution, significantly increases the object-side field of view, raising the number of fields of view per imaging from the original FN26 to at least FN32. Without considering image stitching and overlap, the number of images required is reduced from 100 to 64 or even less, improving the efficiency of rapid pathological slide reading to a certain extent. Furthermore, it achieves apochromatic correction in the 480nm to 644nm wavelength range, effectively correcting spherical aberration, coma, astigmatism, field curvature, distortion, transverse chromatic aberration, and axial chromatic aberration, meeting the requirements of a plan-field apochromatic objective and achieving the technical requirements of a wide field of view (i.e., large field number and low magnification) and high numerical aperture.

[0064] The above embodiments merely illustrate several implementation methods of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the invention patent. It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of the present invention. These are all equivalent modifications and improvements made to the above embodiments based on the essential technology of the present invention, and all of these fall within the protection scope of the present invention.

Claims

1. A high-throughput microscope objective lens, characterized in that: It consists of a first lens, a second lens, a first lens group, a second lens group, a third lens group, a fourth lens group, a twelfth lens, and a fifth lens group arranged coaxially from the object side to the image side; the first lens has a concave surface facing the object side and a convex surface facing the image side; The second lens has a concave surface facing the object and a convex surface facing the image. The second lens is a positive meniscus lens and has positive optical power. The first lens group consists of a third lens, a fourth lens, and a fifth lens that are cemented together in sequence. The first lens group has positive optical power and has a convex surface facing both the object and image. The second lens group consists of a sixth lens and a seventh lens that are glued together in sequence. The second lens group has positive optical power. The second lens group has a convex surface facing the object side and a convex surface facing the image side. The third lens group is composed of an eighth lens and a ninth lens that are glued together in sequence. The third lens group has positive optical power and is convex when facing the object and when facing the image. The fourth lens group consists of a tenth lens and an eleventh lens that are glued together in sequence. The fourth lens group has negative optical power. The fourth lens group has a convex surface facing the object side and a concave surface facing the image side. The twelfth lens has a concave surface facing the object and a convex surface facing the image, and has negative optical power; the fifth lens group is composed of the thirteenth lens and the fourteenth lens, which are cemented together in sequence. The fifth lens group has positive optical power, and has a concave surface facing the object and a convex surface facing the image. At least two of the lenses in the first lens group, the second lens group, the third lens group, the fourth lens group, and the fifth lens group have different refractive indices.

2. The high-throughput microscope objective lens according to claim 1, characterized in that: The first lens is a Qiming lens.

3. The high-throughput microscope objective lens according to claim 1, characterized in that: The third lens has a convex surface facing both the object and image sides; the fourth lens has a concave surface facing both the object and image sides; and the fifth lens has a convex surface facing both the object and image sides.

4. The high-throughput microscope objective lens according to claim 1, characterized in that: The sixth lens has a convex surface facing the object and a concave surface facing the image, while the seventh lens has a convex surface facing both the object and image.

5. The high-throughput microscope objective lens according to claim 1, characterized in that: The eighth lens has a convex surface facing the object and a concave surface facing the image, while the ninth lens has a convex surface facing both the object and image.

6. The high-throughput microscope objective lens according to claim 1, characterized in that: The tenth lens has a convex surface facing the object and a concave surface facing the image, while the eleventh lens has a convex surface facing the object and a concave surface facing the image.

7. The high-throughput microscope objective lens according to claim 1, characterized in that: The twelfth lens is a double meniscus lens.

8. The high-throughput microscope objective lens according to claim 1, characterized in that: The thirteenth lens has a concave surface facing both the object and image sides, while the fourteenth lens has a convex surface facing both the object and image sides.

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