An anti-aging and firming composition and its application

Abstract

The present invention discloses an anti-aging and firming composition and its application, which includes the components of golden mango hydrating essence and alpinia zerumbet natural revitalizing essence, and also includes any one of the components of optimal peptide, gene elasticity code, Matrixyl 3000 and argireline. The ratio of each component is: 1:1:1 (V / V / V). The anti-aging and firming composition provided by the present invention has no cytotoxicity to fibroblasts, shows a significant improvement in the elastin content and CollagenⅠ content of fibroblasts induced by UVA radiation, has a significant down-regulating effect on the increase in the MMP-1 content level of fibroblasts induced by ultraviolet radiation, has a certain firming and anti-wrinkle effect, and the skin firmness is improved.

Description

Technical Field

[0001] The invention relates to the technical field of skin care products, and in particular to an anti-aging and firming composition and application thereof. Background Art

[0002] As the human body ages, its functions decline, and more and more skin problems will appear, such as spots, dull yellow, wrinkles, etc. Aging is something that everyone is worried about. In addition to the factors of skin cell proliferation and slow metabolism caused by the aging of the body itself, environmental factors also have a great impact on skin aging. The skin is a natural barrier against the invasion of the external environment. However, it will inevitably be damaged when exposed to the external environment for a long time. The most harmful factors are ultraviolet rays, infrared rays, pollution and smoke. When the skin is exposed to the external environment, the cells will continuously produce free radicals, resulting in a large increase in free radicals on the face and body, triggering the activity of metalloproteinases in the cells, which in turn damages and cuts off the collagen cross-linked connective tissue of the skin.

[0003] Starting at around age 30, the human body's metabolism gradually slows down, the function of the sebaceous glands and sweat glands slowly declines, and the internal fiber reserves supporting the skin's surface are gradually exhausted, causing the skin's internal outward tension to continue to decrease, and local areas begin to sink, causing wrinkles to appear and become increasingly obvious.

[0004] Therefore, many anti-aging skin care products have been launched, and they are of various types. At present, there are many lifting and firming cosmetics on the market, and in order to make these products absorb and work, most of them use chemicals to a greater or lesser extent, which will cause irreversible damage to the skin if used for a long time; while pure plant cosmetics are poorly absorbed, and their effects are relatively slow and single. In addition, although plant-based anti-aging products are safe, hypoallergenic, and non-irritating to the skin, they are especially suitable for sensitive, dry, wrinkled skin, but their effects are not significant, and they are costly and difficult to prepare. Summary of the invention

[0005] The purpose of the present invention is to provide an anti-aging and firming composition and its application in view of the deficiencies of the prior art. The composition has no fibroblast toxicity, significantly improves the elastin content and Collagen I content of fibroblasts induced by UVA radiation, and significantly downregulates the elevated MMP-1 content level of fibroblasts induced by ultraviolet radiation, has firming and anti-wrinkle effects, and improves skin firmness.

[0006] The first object of the present invention is to provide an anti-aging and firming composition, comprising a golden mango water light element component, an Alpinia officinalis natural activating element component, and any one of a texture peptide component, a muscle elastic code component, a base peptide 3000 component and an arginine component, and the distribution ratio of each component is: 1:1:1 (V / V / V).

[0007] Furthermore, the mass percentage composition of the Gene Elasticity Secret includes 2.0% - 3.0% yeast polypeptides, 0.45 - 0.81% phenoxyethanol, 0.05 - 0.09% ethylhexylglycerin, 94.1 - 96.0% water, and 1.5% - 2.0% hydrolyzed RNA.

[0008] Furthermore, the mass percentage composition of the Golden Mango Hydrating Essence includes 62.5 - 67.5% water, 25 - 30% glycerol, and 6.5 - 8.5% mango fruit extract.

[0009] Furthermore, the mass percentage composition of the Alpinia zerumbet Natural Revitalizing Essence includes 45 - 50% 1,2 - propanediol, 45 - 50% water, and 0.1 - 1% Alpinia zerumbet leaf extract.

[0010] Furthermore, the mass percentage composition of the Anti - wrinkle Peptide includes 50 - 75% glycerol, 0.09 - 0.13% palmitoyl tripeptide - 5, and 25 - 50% water.

[0011] Furthermore, the mass percentage composition of the Argireline includes 99.45% water, 0.05% acetyl hexapeptide - 8, and 0.5% caprylyl glycol.

[0012] Furthermore, the Argireline also includes ethylhexylglycerin.

[0013] The second objective of the present invention is to provide the application of the anti - aging and firming composition in cosmetics.

[0014] The third objective of the present invention is to provide an anti - aging and firming cream, comprising the firming and anti - aging composition described in any one of the above. The mass percentage composition of the cream includes 64.45% water, 4.00% glycerol, 0.50% erythritol, 0.20% allantoin, 0.20% xanthan gum, 0.50% panthenol, 2.00% butanediol, 0.60% acryloyldimethyltaurate / VP copolymer, 3.00% isopropyl myristate, 2.50% cetearyl glucoside - sorbitan olivate - cetearyl alcohol, 2.00% phytosterol oleate - phytosterols, 2.50% shea butter, 1.50% cetearyl alcohol, 0.50% tocopheryl acetate, 1.50% polydimethylsiloxane, 4.00% caprylic / capric triglyceride, 3.00% cetyl palmitate, 0.50% 1,2 - hexanediol, 0.05% ethylhexylglycerin, 0.50% p - hydroxyacetophenone, 2.00% Golden Mango Hydrating Essence, 2.00% Alpinia zerumbet Natural Revitalizing Essence, and any one of 2.00% Anti - wrinkle Peptide, Gene Elasticity Secret, Matrixyl 3000, or Argireline.

[0015] The beneficial effects of the present invention include the following aspects:

[0016] The anti-aging and firming composition provided by the present invention has no cytotoxicity to fibroblasts, significantly increases the elastin content and CollagenⅠ content in fibroblasts induced by UVA radiation, significantly down-regulates the elevated MMP-1 content level in fibroblasts induced by ultraviolet radiation, has a certain firming and anti-wrinkle effect, and improves skin firmness. Description of the Drawings

[0017] Figure 1 It is the cell viability graph in Example 1;

[0018] Figure 2 It is the trend graph of the change in elastin content in Example 1;

[0019] Figure 3 It is the trend graph of the change in type I collagen content in Example 2;

[0020] Figure 4 It is the trend graph of the change in MMP-1 content in Example 2;

[0021] Figure 5 It is the comparison graph of the Primos CR wrinkle area detection results in Example 3;

[0022] Figure 6 It is the comparison graph of the skin firmness RO detection results in Example 3;

[0023] Figure 7 It is the sample photo in Example 3. Detailed Embodiments

[0024] To facilitate the understanding of the present invention, the present invention will be described more comprehensively below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0025] Example 1

[0026] 1. The sample information is as follows:

[0027] Table 1 Grouping Information of Test Samples

[0028]

[0029] 2. Test Items

[0030] Detection of elastin content.

[0031] 3. Test Materials

[0032] 3.1 Test System

[0033] The cells used in this test were fibroblasts (HFF-1), which were purchased from the Cell Bank of the Chinese Academy of Sciences.

[0034] 3.2 Reagents

[0035] High-glucose DMEM culture medium (Gibco), fetal bovine serum (Gibco), PBS (Gibco), MTT (Sigma), DMSO (Sinopharm), and lumbar protease (Gibco).

[0036] 3.3 Main Equipment

[0037] CO2 incubator (Thermo, 160i), biosafety cabinet (ESCO, LA2-6A1), inverted microscope (Leica, DMi8), microplate reader (Tecan, Spark), microvibrator (Qilin Bell, TS-92).

[0038] 3.4 Test sample information

[0039] Table 2 Test sample information

[0040]

[0041] 4. Experimental methods

[0042] 4.1 Cytotoxicity assay

[0043] 1) Cell seeding: 8×10 3 The cells were seeded into a 96-well plate at a seeding density of 100 cells / well and incubated overnight in an incubator (37° C., 5% CO 2 ).

[0044] 2) Experimental grouping: The experiment set up a zero-adjustment group, a control group, a positive control group and a sample group. In the sample group, 8 concentration gradients were set for each sample, and 3 replicate wells were set under each concentration gradient.

[0045] 3) Solution preparation: Prepare sample working solutions of different concentrations according to the test concentration setting table (Table 3).

[0046] Table 3 Test sample information

[0047]

[0048] 4) Administration: Administration was performed when the cell plating rate in the 96-well plate reached 40%-60%. 200 μL of culture solution containing 10% PBS was added to each well of the control group; 200 μL of culture solution containing 10% DMSO was added to each well of the positive control group; 200 μL of culture solution containing the corresponding concentration of sample was added to each well of the sample group; the zero adjustment group had no cell inoculation and only 200 μL of cell culture solution was added. After administration, the 96-well plate was placed in an incubator (37°C, 5% CO2) for incubation.

[0049] 5) Detection: After the cells were incubated for 24 h, the supernatant was discarded, and the medium containing 0.5 mg / mL MTT was added. The cells were incubated at 37 °C in the dark for 4 h. After incubation, the supernatant was discarded, and 100 μL DMSO was added to each well. The OD value was measured at 490 nm.

[0050] 6) Calculation of relative cell viability: Calculate according to the formula

[0051]

[0052] 4.2 Detection of elastin content

[0053] 4.2.1 Experimental design

[0054] The specific experimental grouping is shown in Table 4.

[0055] Table 4 Experimental design

[0056]

[0057] 4.2.2 Experimental method

[0058] 1) Cell seeding: Seed the cells at an appropriate seeding density (5×10 4 / well) into a 24-well plate and incubate overnight in an incubator (37 °C, 5% CO2).

[0059] 2) Experimental grouping: Set up a blank control group, a negative control group, and a sample group. Three concentration gradients were set in the sample group, and three replicate wells were set under each concentration gradient.

[0060] 3) Solution preparation: Prepare working solutions of the test substances at different concentrations according to the test concentration setting table (Table 4).

[0061] 4) UVA irradiation: After the cells were cultured for 24 h, the negative control group and the sample group received UVA irradiation with a total dose of 9 J / cm 2 . At the same time, the blank control group was placed in the same environment (UVA irradiation dose of 0 J / cm 2 ).

[0062] 5) Drug administration: Administer the drugs when the cell confluence in the 24-well plate reaches 40%-60%. Add 1 mL of cell culture medium to each well in the blank control group and the negative control group; add 1 mL of the culture medium containing the corresponding concentration of the test substance to each well in the sample group; after drug administration, place the 24-well plate in an incubator (37 °C, 5% CO2) for culture.

[0063] 6) Collection of cell supernatant: After incubation for 24 h, collect the cell culture supernatant into an EP tube and store it frozen at -80 °C in a refrigerator.

[0064] 7) ELISA assay: The content of Elastin was detected and analyzed according to the operation manual of the ELISA assay kit.

[0065] 5. Experimental Results

[0066] 5.1 Cytotoxicity

[0067] Eight dosing concentrations were set for the samples, and a cytotoxicity detection experiment was carried out on fibroblasts. The MTT detection results are shown in Table 5.

[0068] Table 5 MTT detection results of the samples

[0069]

[0070]

[0071] Taking the eight concentrations selected for the anti-wrinkle & firming combination (Samples 1 - 4) as the abscissa and the relative cell viability value as the ordinate, a relative cell viability graph was plotted (see Figure 1 ).

[0072] Therefore, according to the MTT results, among the anti-wrinkle & firming combination (Samples 1 - 4), Sample 4 - 1 combination did not show fibroblast cytotoxicity within the concentration range of 1.25%, Sample 4 - 2 did not show fibroblast cytotoxicity within the concentration range of 10.00%, Sample 4 - 3 combination did not show fibroblast cytotoxicity within the concentration range of 5.00%, and Sample 4 - 4 combination did not show fibroblast cytotoxicity within the concentration range of 10.00%.

[0073] 5.2 Detection results of Elastin content

[0074] Based on the experimental method, cell supernatants were collected for the detection of elastin content. The detection results are shown in Table 6, and the change trend is as Figure 2 shown.

[0075] Table 6 Summary table of elastin data

[0076]

[0077] Note: When performing statistical analysis using the t-test method, compared with the BC group, the significance of the sample group and the PC group is indicated by *, p-value < 0.05 is indicated by *, and p-value < 0.01 is indicated by **.

[0078] In the detection results of elastin content, compared with the BC group, the total dose received by NC group fibroblasts was 9 J / cm 2After UVA irradiation, the elastin content level was significantly downregulated (P<0.05), indicating that UVA stimulation is highly effective. Compared with the NC group, in the wrinkle removal and firming combination (samples 1 to 4), sample 4-1 combination was at 1.25%

[0079] At the concentration of (%, V / V), this product significantly increased the elastin content of fibroblasts induced by UVA radiation.

[0080] 6. Conclusion

[0081] In the elastin content test results, compared with the NC group, in the anti-wrinkle & firming combination (samples 1 to 4), the sample 4-1 combination significantly increased the elastin content of UVA radiation-induced fibroblasts at a concentration of 1.25% (%, V / V), and had a certain firming and anti-wrinkle effect.

[0082] Example 2

[0083] 1. Sample information

[0084] As in Example 1.

[0085] 2. Test items

[0086] Detection of type Ⅰ collagen content.

[0087] 3. Test materials

[0088] 3.1 Test system

[0089] The cells used in this test were fibroblasts (HFF-1), which were purchased from the Cell Bank of the Chinese Academy of Sciences.

[0090] 3.2 Reagents

[0091] High-glucose DMEM culture medium (Gibco), fetal bovine serum (Gibco), PBS (Gibco), MTT (Sigma), DMSO (Sinopharm), and lumbar protease (Gibco).

[0092] 3.3 Main Equipment

[0093] CO2 incubator (Thermo, 160i), biosafety cabinet (ESCO, LA2-6A1), inverted microscope (Leica, DMi8), microplate reader (Tecan, Spark), microvibrator (Qilin Bell, TS-92).

[0094] 3.4 Test sample information

[0095] As in Example 1.

[0096] 4. Experimental methods

[0097] 4.1 Cytotoxicity assay

[0098] 1) Cell seeding: Seed cells into a 96-well plate at a seeding density of 8×10 3 cells / well and incubate overnight in an incubator (37°C, 5% CO2).

[0099] 2) Experimental grouping: Set up a zero control group, a control group, a positive control group, and a sample group. In the sample group, set 8 concentration gradients for each sample, and set 3 replicate wells for each concentration gradient.

[0100] 3) Solution preparation: Prepare sample working solutions at different concentrations according to the test concentration setting table in Example 1.

[0101] 4) Administration: Administer drugs when the cell confluence rate in the 96-well plate reaches 40%-60%. Add 200 μL of culture medium containing 10% PBS to each well in the control group; add 200 μL of culture medium containing 10% DMSO to each well in the positive control group; add 200 μL of culture medium containing the corresponding concentration of the sample to each well in the sample group; in the zero control group, no cells are seeded, and only 200 μL of cell culture medium is added. After drug administration, place the 96-well plate in an incubator (37°C, 5% CO2) for culture.

[0102] 5) Detection: After incubating the cells for 24 h, discard the supernatant, add a medium containing 0.5 mg / mL of MTT, and incubate at 37°C in the dark for 4 h. After the incubation, discard the supernatant, add 100 μL of DMSO to each well, and read the OD value at 490 nm.

[0103] 6) Calculation of relative cell viability: Calculate according to the formula in Example 1

[0104] 4.2 Detection of type I collagen and MMP-1

[0105] 1) Cell seeding: Seed cells into a 24-well plate at an appropriate seeding density (5×10 4 cells / well) and incubate overnight in an incubator (37°C, 5% CO2).

[0106] 2) Experimental grouping: Set up a blank control group, a negative control group, a positive control group, and a sample group. Set 3 concentration gradients in the sample group.

[0107] 3) Solution preparation: Prepare test item working solutions at different concentrations according to the test concentration setting table (7).

[0108] Table 7 Experimental design

[0109]

[0110] 4) Administration: Administration is carried out when the cell seeding rate in the 24-well plate reaches 40%-60%. For the blank control group and the negative control group, 1 mL of cell culture medium is added to each well; for the positive control group, 1 mL of culture medium containing 100 μg / mL of vitamin C and 7 μg / mL of vitamin E is added to each well; for the sample group, 1 mL of culture medium containing the corresponding concentration of the test substance is added to each well. After administration, the 24-well plate is placed in an incubator (37°C, 5% CO2) for culture.

[0111] 5) UVA irradiation: After 24 h of cell culture, the negative control group, the positive control group, and the sample group are irradiated with UVA with a total dose of 9 J / cm 2 , and at the same time, the blank control group is placed in the same environment (UVA irradiation dose is 0 J / cm 2 ).

[0112] 6) Collection of cell supernatant: After 24 h of incubation culture, the cell culture supernatant is collected into an EP tube and stored frozen in a -80°C refrigerator.

[0113] 7) ELISA detection: Detection and analysis are carried out according to the operation manuals of different ELISA kits.

[0114] 5. Experimental results

[0115] 5.1 Cytotoxicity

[0116] Eight dosing concentrations of the sample are set, and a cytotoxicity detection experiment is carried out on fibroblasts. The MTT detection results are shown in Table 8.

[0117] Table 8 MTT detection results of the sample

[0118]

[0119] Taking the eight concentrations selected for the sample anti-wrinkle & firming combination (Samples 1-4) as the abscissa and the relative cell viability value as the ordinate, a relative cell viability graph is plotted (see Figure 1 ).

[0120] Therefore, according to the MTT results, among the sample anti-wrinkle & firming combinations (Samples 1-4), the Sample 4-1 combination did not show cytotoxicity to fibroblasts within the concentration range of 1.25%, the Sample 4-2 combination did not show cytotoxicity to fibroblasts within the concentration range of 10.00%, the Sample 4-3 combination did not show cytotoxicity to fibroblasts within the concentration range of 5.00%, and the Sample 4-4 combination did not show cytotoxicity to fibroblasts within the concentration range of 10.00%.

[0121] 5.2 Detection results of type I collagen content

[0122] Based on the experimental method, cell supernatants were collected and the content of type I collagen was detected. The detection results are shown in Table 9, and the trend is as Figure 3 shown.

[0123] Table 9 Summary of type I collagen data

[0124]

[0125] Note: When using the t-test method for statistical analysis, compared with the NC group and the BC group, the significance is indicated by #, p-value < 0.05 is indicated by #, and p-value < 0.01 is indicated by ##. When comparing the sample group and the PC group with the NC group, the significance is indicated by *, p-value < 0.05 is indicated by *, and p-value < 0.01 is indicated by **.

[0126] In the detection results of type I collagen content, compared with the BC group, after the fibroblasts in the NC group received UVA radiation with a total dose of 9 J / cm 2 , the content level of Collagen I was significantly down-regulated (P < 0.05), indicating that the UVA stimulation was effective.

[0127] Compared with the NC group, among the sample anti-wrinkle & firming combinations (Samples 1 - 4), the Sample 4-1 combination showed a significant promoting effect on the content of Collagen I induced by UVA radiation in fibroblasts at a concentration of 1.25% (%, V / V), the Sample 4-2 combination showed a significant promoting effect on the content of Collagen I induced by UVA radiation in fibroblasts at a concentration of 2.00% (%, V / V), and the Sample 4-4 combination showed a significant promoting effect on the content of Collagen I induced by UVA radiation in fibroblasts at a concentration of 2.00% (%, V / V).

[0128] 5.3 Detection results of MMP-1

[0129] According to the specific experimental method, the content of MMP-1 was detected. The detection results are shown in Table 10, and the trend is as Figure 4 shown, as follows:

[0130] Table 10 Summary of MMP-1 results

[0131]

[0132] Note: When using the t-test method for statistical analysis, compared with the NC group and the BC group, the significance is indicated by #, p-value < 0.05 is indicated by #, and p-value < 0.01 is indicated by ##. When comparing the sample group and the PC group with the NC group, the significance is indicated by *, p-value < 0.05 is indicated by *, and p-value < 0.01 is indicated by **.

[0133] The results showed that, compared with the BC group, after the fibroblasts in the NC group were irradiated with UVA at a total dose of 9 J / cm 2 , the content level of MMP-1 increased significantly, indicating that the ultraviolet stimulation was effective.

[0134] Compared with the NC group, vitamin C and vitamin E in the PC group could significantly reduce the content level of MMP-1.

[0135] Compared with the NC group, among the anti-wrinkle & firming combination of samples (Samples 1-4), the combination of Sample 4-1 at a concentration of 1.25% (%, V / V), and the combinations of Sample 4-2, Sample 4-3, and Sample 4-4 at a concentration of 2.00% (%, V / V) all had a significant down-regulating effect on the increase in the content level of MMP-1 induced by ultraviolet radiation in fibroblasts (P<0.05).

[0136] 6. Conclusion

[0137] In the test results of the content of type I collagen, compared with the NC group, among the anti-wrinkle & firming combination of samples (Samples 1-4), the combination of Sample 4-1 at a concentration of 1.25% (%, V / V) showed a significant promoting effect on the content of Collagen I in fibroblasts induced by UVA radiation, and the combinations of Sample 4-2 and Sample 4-4 at a concentration of 2.00% (%, V / V) both showed a significant promoting effect on the content of Collagen I in fibroblasts induced by UVA radiation, having a certain firming and anti-wrinkle effect.

[0138] In the test results of the content of MMP-1, compared with the NC group, among the anti-wrinkle & firming combination of samples (Combination of Samples 1-4), the combination of Sample 4-1 at a concentration of 1.25% (%, V / V), and the combinations of 4-2, 4-3, and 4-4 at a concentration of 2.00% (%, V / V) all had a significant down-regulating effect on the increase in the content level of MMP-1 induced by ultraviolet radiation in fibroblasts (P<0.05), having a certain firming and anti-wrinkle effect.

[0139] Example 3

[0140] 1. Detection Content

[0141] 1.1 Anti-wrinkle and firming human body efficacy test. Continuously use the product for 14 days and 28 days to test the efficacy of the product.

[0142] 1.2 As Figure 7As shown, the product is an anti-aging and firming cream, and its mass percentage composition includes 64.45% water, 4.00% glycerin, 0.50% erythritol, 0.20% allantoin, 0.20% xanthan gum, 0.50% panthenol, 2.00% butanediol, 0.60% acryloyldimethyltaurate / VP copolymer, 3.00% isopropyl myristate, 2.50% cetearyl glucoside-sorbitan olivate-ceteareth, 2.00% phytosteryl oleate-phytosterols, 2.50% shea butter, 1.50% ceteareth, 0.50% tocopheryl acetate, 1.50% dimethicone, 4.00% caprylic / capric triglyceride, 3.00% cetyl palmitate, 0.50% 1,2-hexanediol, 0.05% ethylhexylglycerin, 0.50% p-hydroxyacetophenone, 2.00% mango hydrating essence, 2.00% Alpinia zerumbet natural revitalizing essence and 2.00% argireline.

[0143] 1.3 The number of test subjects is 30.

[0144] The scheme is based on referring to T / SHRH 018-2019 Clinical Evaluation Method for the Efficacy of Cosmetics in Improving Crow's Feet Wrinkles;

[0145] T / TDCA003-2021 Test Method for the Firming Efficacy of Cosmetics;

[0146] Liu Wei, Zhou Lin, Zhao Hua. Evaluation of Cosmetic Efficacy (X 111) - Consumer Use Test [J]. China Surfactant Detergent & Cosmetics, 2021, 51(06):485-490.

[0147] 2. Detection Method

[0148] Select 30 Chinese healthy male / female subjects aged 25-60 years old with crow's feet wrinkle grades of 3-5 and loose and inelastic skin in the test area. Using the before-and-after control method, the skin elasticity and skin firmness of the subjects are measured and local photos of the subjects are taken before using the product, after continuously using the product for 14 days, and 28 days. The evaluation results before and after using the product are compared through statistical test methods to determine whether there are statistical differences.

[0149] 3. Detection Sites

[0150] (1) Primos CR wrinkle area - crow's feet

[0151] (2) Primos CR volume - crow's feet

[0152] (3) Skin elasticity R5 - full face

[0153] (4) Skin firmness RO - full face

[0154] (5) Subject self-evaluation - full face

[0155] 4. Detection Indicators

[0156] Table 11 Functional Detection

[0157]

[0158]

[0159] 5. Detection Environment

[0160] The environmental temperature for this test is 20.2°C - 22.0°C, and the relative humidity is 44.9%RH - 60.0%, meeting the requirements of the scheme design.

[0161] 6. Detection Process

[0162] Table 12 Detection Process Arrangement

[0163]

[0164]

[0165] 7. Detection Process

[0166] The statistical analysis software is SPSS. If the values are normally distributed, the t - test method is used for statistical analysis; if the values are non - normally distributed, the rank - sum test method is used for statistical analysis.

[0167] Both statistical methods use two - tailed tests, and the significance level = 0.05.

[0168] The calculation formula is as follows:

[0169] The △ (difference) of using product D14 = D14 - D0

[0170] The △ (difference) of using product D28 = D28 - D0

[0171] The change rate of using the product for 14 days = (D14 - D0) / D0 × 100%.

[0172] The change rate of using the product for 28 days = (D28 - D0) / D0 × 100%.

[0173] In the formula, D0 - - the baseline value of skin parameters before using the product in the test area.

[0174] D14 - - the value of skin parameters after using the product for 14 days in the test area.

[0175] D28 - - the value of skin parameters after using the product for 28 days in the test area.

[0176] N - - the number of subjects.

[0177] 8. Detection Results

[0178] 8.1 Sample Completion Status

[0179] 30 subjects were enrolled, and finally 30 subjects were counted. All were female, aged 32 to 55 years old, with an average age of 46.93 ± 1.04 years. After cleaning the face, it was applied to the face, once in the morning and once in the evening.

[0180] 8.2 Product Usage Feedback

[0181] For subjects who continuously used the product for 14 days and 28 days as required, by asking and examining, and recording whether there were skin reactions or systemic adverse reactions during the test period of the subjects. According to the "Technical Specifications for Cosmetics Safety (2015 Edition)", no adverse reactions were found according to the results of adverse reaction records.

[0182] 8.3 Instrument Detection Results and Statistical Analysis Results

[0183] 8.3.1 Primos CR Wrinkled Area, such as Figure 5 。

[0184] Table 13 Detection Results of Primos CR Wrinkled Area 1

[0185]

[0186] * The data in the table are mean ± standard error, and the Youcui Facial Rejuvenation is the finished product of the present invention;

[0187] Table 14 Detection Results of Primos CR Wrinkled Area 2

[0188]

[0189] * Statistical method: Analyzed by t - test method, the test level α = 0.05.

[0190] * Significance annotation method: "n.s" indicates no statistical difference, p ≥ 0.05; p < 0.05 indicates a significant difference ("*" indicates 0.01 ≤ p < 0.05; "**" indicates 0.001 ≤ p < 0.01; "***" indicates p < 0.001).

[0191] * Number of people using the product = 30

[0192] Result Interpretation:

[0193] For the subjects who used the product Youcui Facial Rejuvenation for 14 days and 28 days, the Primos CR wrinkled area in the tested area was basically the same as the baseline value (p ≥ 0.05).

[0194] 8.3.2 Skin Elasticity RO

[0195] Table 15 Skin Elasticity RO Test Results 1

[0196]

[0197] *Data in the table are mean ± standard error

[0198] Table 16 Skin Elasticity RO Test Results 2

[0199]

[0200] Statistical method: Analysis was performed using the t-test method, with a significance level of α = 0.05.

[0201] *Significance annotation method: "n.s." indicates no statistical difference, p ≥ 0.05; p < 0.05 indicates a significant difference ("*" indicates 0.01 ≤ p < 0.05; "**" indicates 0.001 ≤ p < 0.01; "***" indicates p < 0.001).

[0202] *Number of people using the product = 30;

[0203] Result interpretation:

[0204] As Figure 6 shown in the comparison chart of skin elasticity RO test results, after 14 days of using the product Youcui Shuyan, the skin firmness RO of the tested area showed a significant decrease (0.001 ≤ p < 0.01) compared with the baseline value, with a decrease rate of 7.69%. After 28 days of using the product Youcui Shuyan, the skin firmness RO of the tested area showed a significant decrease (p < 0.001) compared with the baseline value, with a decrease rate of 10.90%.

[0205] Skin firmness RO: The smaller the firmness RO, the better the skin firmness.

[0206] 9. Self-evaluation by subjects

[0207] After 28 days of continuous use of the product Youcui Shuyan, 96.67% of the subjects felt that the product had a water-locking and moisturizing effect after using it; 186.67% of the subjects felt that the product could increase skin elasticity; 86.67% of the subjects felt that the product could improve skin firmness; 86.67% of the subjects felt that the product could fade wrinkles; 86.67% of the subjects felt that the product had an anti-wrinkle effect; 96.67% of the subjects evaluated that this product was mild and non-irritating; 96.67% of the subjects were generally satisfied with this product.

[0208] 10. Safety evaluation results

[0209] For the subjects who continuously used the product for 14 days and 28 days as required, through inquiries and examinations, record whether there were skin reactions or systemic adverse reactions during the test period of the subjects, including the manifestations, occurrence time, treatment measures and outcomes of adverse events, and make a judgment on the relationship between the adverse events and the samples used. The details are shown in the following table:

[0210] Skin reaction Grading D14 D28 No reaction 0 30 cases 30 cases Weak erythema 1 0 cases 0 cases Erythema, infiltration, papules 2 0 cases 0 cases Erythema, edema, papules, vesicles 3 0 cases 0 cases Erythema, edema, bullae 4 0 cases 0 cases

[0211] 11. Subject Information

[0212]

[0213]

Claims

1. An anti-aging and firming composition, characterized in that: It includes the components of Golden Mango Hydrating Element and Alpinia zerumbet Natural Revitalizing Element, and also includes any one of the components of Super Collagen Peptide, Gene Elasticity Code, Matrixyl 3000 and Argireline, and the ratio of each component is 1:1:1 (V / V / V); The mass percentage composition of the Gene Elasticity Code includes 2.0% - 3.0% yeast polypeptides, 0.45 - 0.81% phenoxyethanol, 0.05 - 0.09% ethylhexylglycerin, 94.1 - 96.0% water and 1.5% - 2.0% hydrolyzed RNA; The mass percentage composition of the Golden Mango Hydrating Element includes 62.5 - 67.5% water, 25 - 30% glycerol and 6.5 - 8.5% mango fruit extract; The mass percentage composition of the Alpinia zerumbet Natural Revitalizing Element includes 45 - 50% 1,2 - propanediol, 45 - 50% water and 0.1 - 1% Alpinia zerumbet leaf extract; The mass percentage composition of the Super Collagen Peptide includes 50 - 75% glycerol, 0.09 - 0.13% palmitoyl tripeptide - 5 and 25 - 50% water; The mass percentage composition of the Argireline includes 99.45% water, 0.05% acetyl hexapeptide - 8 and 0.5% caprylyl glycol.

2. The anti-aging and firming composition according to claim 1, wherein: The Argireline also includes ethylhexylglycerin.

3. The application of an anti - aging and firming composition as described in any one of claims 1 - 2 in cosmetics.

4. An anti-aging and firming cream, characterized in that: It includes the firming and anti - aging composition as described in any one of claims 1 - 2. The mass percentage composition of the cream includes 64.45% water, 4.00% glycerol, 0.50% erythritol, 0.20% allantoin, 0.20% xanthan gum, 0.50% panthenol, 2.00% butanediol, 0.60% acryloyldimethyltaurate / VP copolymer, 3.00% isopropyl myristate, 2.50% cetearyl glucoside - sorbitan oleate - cetearyl alcohol, 2.00% phytosterol oleate - phytosterols, 2.50% shea butter, 1.50% cetearyl alcohol, 0.50% tocopheryl acetate, 1.50% polydimethylsiloxane, 4.00% caprylic / capric triglyceride, 3.00% cetyl palmitate, 0.50% 1,2 - hexanediol, 0.05% ethylhexylglycerin, 0.50% p - hydroxyacetophenone, 2.00% Golden Mango Hydrating Element, 2.00% Alpinia zerumbet Natural Revitalizing Element and 2.00% any one of Super Collagen Peptide, Gene Elasticity Code, Matrixyl 3000 or Argireline.

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