An optical detection device for a PCR instrument

Abstract

This invention discloses an optical detection device for a PCR instrument, comprising a light source assembly, a light-diffusing plate, multiple first lenses, multiple second lenses, a converging lens, a filter component, and a collection component. The light source assembly emits excitation light and directs it into the light-diffusing plate. The excitation light passes through the light-diffusing plate and enters a sample tube. The sample in the sample tube then emits emitted light. The first lenses are located between the light-diffusing plate and the sample tube, the second lenses are located above the light-diffusing plate, and the converging lens is located above the multiple second lenses. The emitted light passes through the filter component and enters the collection component. When the excitation light enters the light-diffusing plate, it is reflected by the first and second reflective films and then enters the sample tube. The sample in the sample tube emits emitted light, which is then detected by the collection component, thereby completing the optical detection of the sample in the sample tube. This avoids discrepancies in the detection results and improves the reliability of the detection results.

Description

Technical Field

[0001] This invention relates to the field of optical detection technology, specifically to an optical detection device for a PCR instrument. Background Technology

[0002] When using a PCR instrument to perform qualitative and quantitative analysis on a sample, the usual method is to emit excitation light through a light source. The excitation light illuminates the pre-treated sample, thereby emitting fluorescence from the sample. The emitted fluorescence is collected by the detection instrument, and the sample is then qualitatively and quantitatively analyzed based on the collected results.

[0003] In existing technologies, when multiple identical samples are subjected to qualitative and quantitative detection and analysis in the same batch, the detection results differ, mainly due to two reasons. First, when optically detecting multiple samples, the same light source is used to illuminate the samples sequentially. Due to differences in time intervals and illumination angles, the final detection results differ. If a separate light source is configured for each sample, the structure becomes complex and the cost increases. Second, when the same light source is used to illuminate all samples simultaneously, although the influence of time intervals is reduced and costs are controlled, the excitation light reaching each sample still differs, resulting in differences in the final detection results and reduced reliability. Summary of the Invention

[0004] The purpose of this invention is to provide an optical detection device for a PCR instrument, which solves the problem of reduced reliability of detection results.

[0005] To achieve the above objectives, the present invention provides a technical solution:

[0006] An optical detection device for a PCR instrument, comprising:

[0007] A light source assembly for emitting excitation light;

[0008] A light-diffusing plate is provided, with the light source assembly disposed on at least one side of the light-diffusing plate, allowing the excitation light to enter the light-diffusing plate. A sample tube is disposed below the light-diffusing plate, and the excitation light passes through the light-diffusing plate and enters the sample tube, where the sample to be tested in the sample tube emits emission light. The light-diffusing plate includes a first light-blocking plate and a second light-blocking plate located below the first light-blocking plate. The first light-blocking plate has a plurality of first light-transmitting holes, and the second light-blocking plate has a plurality of second light-transmitting holes. A first reflective film is disposed below the first light-blocking plate, and a second reflective film is disposed above the second light-blocking plate. The first reflective film has a plurality of light-receiving holes, and the second reflective film has a plurality of light-entering holes.

[0009] Multiple first lenses are located between the light-diffusing plate and the sample tube, and each first lens is arranged in a one-to-one correspondence with the sample tube.

[0010] Multiple second lenses are provided, with each second lens positioned above the light-diffusing plate and corresponding to a sample tube.

[0011] A converging lens, located above a plurality of second lenses, is used to converge emitted light;

[0012] A filtering component, located above the converging lens, is used to obtain light of the desired wavelength range in the emitted light;

[0013] The light emitted passes through the filter and enters the acquisition component.

[0014] Optionally, the first lens, the first light-transmitting hole, the second light-transmitting hole, the light-receiving hole, the light-entry hole, and the second lens are coaxially arranged.

[0015] Optionally, the diameter of the first light-transmitting hole is less than or equal to the diameter of the light-receiving hole, the diameter of the light-receiving hole is less than the diameter of the second light-transmitting hole, and the diameter of the second light-transmitting hole is less than or equal to the diameter of the light-entry hole.

[0016] Optionally, it also includes a first mounting plate located below the light-diffusing plate. The first mounting plate has multiple first grooves and multiple second grooves located below the first grooves. The size of the first grooves is smaller than the size of the second grooves. A constant temperature plate is provided below the first mounting plate. The constant temperature plate has multiple first through holes, and each of the first through holes corresponds to a first lens. The first lens is located between the second grooves and the first through holes.

[0017] Optionally, it also includes a second mounting plate, which is located above the light-diffusing plate. The second mounting plate has multiple third slots and multiple fourth slots located above the third slots. The size of the third slots is smaller than the size of the fourth slots. A limiting plate is provided above the second mounting plate. The limiting plate has multiple second through holes, which are arranged one-to-one with the second lens. The second lens is located between the fourth slots and the second through holes.

[0018] Optionally, when the acquisition component is configured as an imaging component, the optical detection device further includes a first lens and a first filter wheel located above the first lens. The first filter wheel includes at least one first receiving space, and the filtering component is located in the first receiving space. The filtering component is coaxially arranged with the first lens and the imaging component, and the emitted light enters the imaging component through the first lens and the filtering component.

[0019] Optionally, when the acquisition component is configured as a hyperspectral imager, the optical detection device further includes a reflector, a second lens, and a second filter wheel located on one side of the second lens. The second filter wheel includes at least one second receiving space, and the filtering component is located in the second receiving space. The filtering component is coaxially arranged with the second lens and the hyperspectral imager. The emitted light is reflected by the reflector and enters the hyperspectral imager through the filtering component and the lens.

[0020] Optionally, the first reflective film includes a diffusion film or a brightness enhancement film, and the second reflective film includes a diffusion film or a brightness enhancement film.

[0021] Optionally, the light source assembly includes a substrate, on which a rotating shaft is disposed, and a turntable is rotatably connected to the rotating shaft, and multiple laser light sources are distributed on the turntable.

[0022] Optionally, two light source assemblies are provided, and the two light source assemblies are symmetrically arranged on both sides of the light-diffusing plate.

[0023] Compared with the prior art, the beneficial effects of the present invention are:

[0024] 1. The optical detection device for a PCR instrument of the present invention includes a light source assembly, a light-diffusing plate, multiple first lenses, multiple second lenses, a converging lens, a filter component, and a collection component. The light source assembly is used to emit excitation light and direct the excitation light into the light-diffusing plate. The excitation light passes through the light-diffusing plate and enters a sample tube. Then, the sample to be tested in the sample tube excites emitted light. The first lenses are located between the light-diffusing plate and the sample tubes and are arranged in a one-to-one correspondence with the sample tubes. The second lenses are located above the light-diffusing plate and are arranged in a one-to-one correspondence with the sample tubes. The converging lens is located above the multiple second lenses and is used to converge the emitted light. The emitted light passes through the filter component and enters the collection component. The light-diffusing plate includes a first light-blocking plate and a second light-blocking plate. The first light-blocking plate has multiple first light-transmitting holes, and the second light-blocking plate has multiple second light-transmitting holes. A filter component is arranged below the first light-blocking plate. A first reflective film and a second reflective film are disposed above a second light-blocking plate. The first reflective film has multiple light-receiving holes, and the second reflective film has multiple light-entry holes. When the excitation light enters the light-diffusing plate, the excitation light is reflected by the first and second reflective films, and then passes through the light-entry holes, the second light-transmitting holes, and the first lens in sequence into the sample tube. The sample to be tested in the sample tube excites emission light. Part of the emission light passes through the first lens, the second light-transmitting hole, the light-entry hole, the light-receiving hole, the first light-transmitting hole, the second lens, the converging lens, and the filter component into the acquisition component. The other part passes through the first lens, the second light-transmitting hole, and the light-entry hole into the light-diffusing plate, and is reflected by the first and second reflective films, and then passes through the light-receiving holes, the first light-transmitting hole, the second lens, the converging lens, and the filter component into the acquisition component, where it is collected and analyzed. The entire process involves homogenizing the excitation light using a homogenizing plate, ensuring that the excitation light reaching each sample tube has nearly uniform and consistent light energy and incident angle. Furthermore, the emitted light is focused by a second lens and a converging lens, and then filtered by a filtering component to extract the desired wavelength before being collected and analyzed by the acquisition component. This optical detection device allows all sample tubes to receive excitation light simultaneously, and the received excitation light is nearly uniform and consistent. This not only avoids the result differences caused by time intervals in the receiving of excitation light from multiple samples in existing technologies, but also ensures that the excitation light reaching all sample tubes is nearly uniform and consistent, thereby avoiding differences in detection results and improving the reliability of the detection results. Moreover, by using a converging lens and acquisition component to focus all emitted light and collect and analyze it at the same time, it further avoids factors that may affect the detection results, such as time intervals in the collection of emitted light from different samples.

[0025] 2. The filter component of the optical detection device for a PCR instrument of the present invention is used to filter the emitted light to obtain the light of the desired wavelength band in the emitted light. Since the emitted light is light with a certain wavelength range, the light energy of different wavelength bands is different. The filter component can capture the wavelength band with the strongest light energy in the emitted light.

[0026] 3. In the optical detection device for a PCR instrument of the present invention, the aperture of the first light-transmitting hole is less than or equal to the aperture of the light-receiving hole, the aperture of the light-receiving hole is less than the aperture of the second light-transmitting hole, and the aperture of the second light-transmitting hole is less than or equal to the aperture of the light-entry hole. Because the aperture of the light inlet is larger than that of the light receiver, less excitation light is emitted upwards from the second reflective film. This reduces the probability of the excitation light being collected by the acquisition component through the light receiver, thus minimizing interference with subsequent light acquisition results. Simultaneously, the larger aperture of the light inlet allows the excitation light from the first reflective film to more easily reach the sample tube and excite the sample inside. Since the aperture of the light receiver is larger than or equal to the aperture of the first light-transmitting aperture, the first light-blocking plate can completely block the light emitted from the first reflective film, preventing excess light from entering the filter component. Similarly, since the aperture of the light inlet is larger than or equal to the aperture of the second light-transmitting aperture, the second light-blocking plate can completely block the light emitted from the second reflective film, preventing excess excitation light from illuminating the outer wall of the sample tube and entering the sample tube. This makes it easier to maintain consistency in the excitation light entering multiple sample tubes.

[0027] 4. The optical detection device for PCR instrument of the present invention can prevent the formation of liquefied droplets on the first lens when the sample to be tested in the sample tube evaporates at high temperature by setting a constant temperature plate, so as to avoid affecting the optical path of excitation light and emission light.

[0028] 5. The light source assembly of the optical detection device for a PCR instrument of the present invention includes a substrate, a rotating shaft is disposed on the substrate, a turntable is rotatably connected to the rotating shaft, and multiple laser light sources are distributed on the turntable. By driving the turntable to rotate, different laser light sources can be aligned with a light-diffusing plate one by one, thereby realizing the switching of different laser light sources to meet different detection requirements.

[0029] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the optical detection device of the present invention;

[0031] Figure 2 This is a partial enlarged view of the optical detection device of the present invention;

[0032] Figure 3This is a simplified schematic diagram of the relevant structure and optical path of a sample tube in the optical detection device of the present invention;

[0033] Figure 4 This is a schematic diagram of the beam homogenizing plate corresponding to a sample tube in the optical detection device of the present invention.

[0034] Figure 5 This is a schematic diagram of the structure of the first reflective film corresponding to a sample tube in the optical detection device of the present invention;

[0035] Figure 6 This is a schematic diagram of the structure of the second reflective film corresponding to a sample tube in the optical detection device of the present invention;

[0036] Figure 7 This is a simplified schematic diagram of the optical detection device of the present invention;

[0037] Figure 8 This is another simplified schematic diagram of the optical detection device of the present invention;

[0038] Figure 9 This is a schematic diagram of the light source assembly of the optical detection device of the present invention.

[0039] In the picture:

[0040] 1. Light source assembly; 1a. Substrate; 1b. Rotating shaft; 1c. Turntable; 1d. Laser light source; 2. Light-diffusing plate; 21. First light-blocking plate; 211. First light-transmitting hole; 22. Second light-blocking plate; 221. Second light-transmitting hole; 23. First reflective film; 231. Light-receiving hole; 24. Second reflective film; 241. Light-entry hole; 3. First lens; 4. Second lens; 5. Converging lens; 6. Filtering component; 7. Acquisition component; 8. First mounting plate; 81. First slot; 82. Second slot; 9. Constant temperature plate; 10. Second mounting plate; 101. Third slot; 102. Fourth slot; 11. Limiting plate; 12. First lens; 13. First filter wheel; 14. Reflector; 15. Second lens; 16. Second filter wheel; 100. Sample tube; 200. Sample plate. Detailed Implementation

[0041] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0042] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0043] Reference Figure 1-9As shown, this embodiment of the present disclosure provides an optical detection device, including: a light source assembly 1, a light-diffusing plate 2, a plurality of first lenses 3, a plurality of second lenses 4, a converging lens 5, a filter component 6, and a collection component 7. The light source assembly 1 is used to emit excitation light. The light-diffusing plate 2 is provided with the light source assembly 1 on at least one side, so that the excitation light enters the light-diffusing plate 2. A sample tube 100 is provided below the light-diffusing plate 2. The excitation light enters the sample tube 100 through the light-diffusing plate 2, and the sample to be tested in the sample tube 100 excites emitted light. The first lenses 3 are located between the light-diffusing plate 2 and the sample tube 100, and are arranged in a one-to-one correspondence with the sample tube 100. The second lenses 4 are located above the light-diffusing plate 2, and are arranged in a one-to-one correspondence with the sample tube 100. The converging lens 5 is located above the plurality of second lenses 4 and is used to converge the emitted light. The emitted light passes through the filter component 6 and enters the collection component 7. The light-diffusing plate 2 includes a first light-blocking plate 21 and a second light-blocking plate 22. The first light-blocking plate 21 has multiple first light-transmitting holes 211, and the second light-blocking plate 22 has multiple second light-transmitting holes 221. A first reflective film 23 is disposed below the first light-blocking plate 21, and a second reflective film 24 is disposed above the second light-blocking plate 22. The first reflective film 23 has multiple light-receiving holes 231, and the second reflective film 24 has multiple light-entry holes 241. When excitation light enters the light-diffusing plate 2, the excitation light is reflected by the first reflective film 23 and the second reflective film 24, and then sequentially passes through the light-entry holes 241, the second light-transmitting holes 221, and the first lens 3 into the sample tube 100. Lens 3 can focus the excitation light into the sample tube 100. The sample to be tested in the sample tube 100 excites emission light. Part of the emission light passes through the first lens 3, the second light-transmitting hole 221, the light-entry hole 241, the light-receiving hole 231, the first light-transmitting hole 211, the second lens 4, the converging lens 5, and the filter component 6 and enters the acquisition component 7. Another part passes through the first lens 3, the second light-transmitting hole 221, and the light-entry hole 241 and enters the light-diffusing plate 2. It is reflected by the first reflective film 23 and the second reflective film 24, and then passes through the light-receiving hole 231, the first light-transmitting hole 211, the second lens 4, the converging lens 5, and the filter component 6 and enters the acquisition component 7, where it is collected and analyzed by the acquisition component 7. The entire process involves homogenizing the excitation light using a homogenizing plate 2, ensuring that the excitation light reaching each sample tube 100 is uniform in both light energy and incident angle. Furthermore, the emitted light is converged by a second lens 4 and a converging lens 5, and then filtered by a filter component 6 to extract the light of the desired wavelength range, which is then collected and analyzed by the acquisition component 7.The optical detection device of this application enables all sample tubes 100 to receive excitation light simultaneously, and the received excitation light is nearly uniform and consistent. On the one hand, it avoids the problem of result differences caused by time intervals in the receiving of excitation light from multiple test samples in the prior art. On the other hand, it also makes the excitation light reaching all sample tubes 100 nearly uniform and consistent, thereby avoiding differences in detection results and improving the reliability of detection results. In addition, by using the converging lens 5 and the acquisition component 7 to converge all emitted light and perform acquisition and analysis at the same time (the acquisition and analysis process is also the detection process), it further avoids the existence of factors that may affect the detection results, such as the time intervals in the acquisition of emitted light from different test samples.

[0044] The first lens 3, the first light-transmitting hole 211, the second light-transmitting hole 221, the light-receiving hole 231, the light-entry hole 241, and the second lens 4 are coaxially arranged, which can facilitate the excitation light and the emission light to enter and exit from the light-diffusing plate 2.

[0045] In addition, the diameter of the first light-transmitting hole 211 is less than or equal to the diameter of the light-receiving hole 231, the diameter of the light-receiving hole 231 is less than the diameter of the second light-transmitting hole 221, and the diameter of the second light-transmitting hole 221 is less than or equal to the diameter of the light-entering hole 241. Since the aperture of the light inlet 241 is larger than that of the light receiver 231, the amount of excitation light emitted upward by the second reflective film 24 will be less. The probability of the excitation light entering the acquisition component 7 through the light receiver 231 and being acquired will also be reduced, thus reducing the interference with the subsequent emission light acquisition results. At the same time, because the aperture of the light inlet 241 is larger than that of the light receiver 231, the excitation light on the first reflective film 23 will more easily pass through the light inlet 241 to reach the sample tube 100 and excite the sample to be tested in the sample tube 100. In other words, the excitation light in the area of ​​the first reflective film 23 from the edge of the light receiver 231 to the edge of the light inlet 241, which is perpendicular to the first reflective film 23, can directly pass through the light inlet 241 without having to undergo multiple reflections between the first reflective film 23 and the second reflective film 24 before it can reach the sample tube 100. The aperture of the light-receiving aperture 231 is greater than or equal to the aperture of the first light-transmitting aperture 211. In this case, the first light-blocking plate 21 can block all the light emitted from the first reflective film 23, preventing excess light from entering the filter component 6. It also prevents the light emitted from the first reflective film 23 from not entering or entering the sample tube 100 in small quantities, thereby avoiding interference with the detection results. The aperture of the light-entry aperture 241 is greater than or equal to the aperture of the second light-transmitting aperture 221. In this case, the second light-blocking plate 22 can block all the light emitted from the second reflective film 24, preventing excess excitation light from irradiating the outer wall of the sample tube 100 and entering the sample tube 100. This makes it easier to control the excitation light entering multiple sample tubes 100 to be consistent.

[0046] The aforementioned filter element 6 can be an emission light filter used to filter the emitted light in order to obtain the light of the desired wavelength band in the emitted light.

[0047] Since the emitted light is light with a certain wavelength range, different wavelengths have different light energies. The filter component 6 can capture the wavelength with the strongest light energy in the emitted light.

[0048] The first light-blocking plate 21 and the second light-blocking plate 22 mentioned above can be metal films, such as aluminum films, but the specific material is not limited here, as long as it can play a role in blocking light.

[0049] In some embodiments, the first reflective film 23 includes a diffusion film or a brightness enhancement film, and the second reflective film 24 includes a diffusion film or a brightness enhancement film.

[0050] Both the first reflective film 23 and the second reflective film 24 can be diffusion films. The excitation light and the emitted light can be reflected between the two diffusion films, and both can diffuse the excitation light and the emitted light. At this time, the diffusion film can emit light uniformly as a whole, and the light uniformity effect is better.

[0051] The first reflective film 23 and the second reflective film 24 here can both be brightness enhancement films. Excitation light and emission light can be reflected between the two brightness enhancement films. That is, when the excitation light and emission light hit the brightness enhancement film, they can be reflected instantly without loss. Since the excitation light and emission light can be reflected multiple times by the two brightness enhancement films, the light uniformity effect is also better.

[0052] The first reflective film 23 and the second reflective film 24 here can also be used as a brightening film for the diffusion film. The excitation light and the emitted light can be reflected between the first reflective film 23 and the second reflective film 24. That is, the excitation light and the emitted light first hit the diffusion film, and most of them will be diffused through the diffusion film. A small part may hit the brightening film and then be reflected to another diffusion film, which also has a better light uniformity effect.

[0053] The first reflective film 23 can be a diffusion film, and the second reflective film 24 can be a brightness enhancement film. The excitation light and the emitted light are reflected between the diffusion film and the brightness enhancement film. That is, when the excitation light and the emitted light hit the diffusion film, the light can be diffused. The diffusion film emits light uniformly as a whole. When the excitation light and the emitted light hit the brightness enhancement film, they can be reflected instantly without loss. Since the excitation light and the emitted light can be diffused and reflected multiple times, the light uniformity effect is also relatively good.

[0054] It should be noted that when the excitation light strikes the diffusion film, the diffusion film emits light uniformly throughout. Therefore, the first light-blocking plate 21 can block the light, preventing excess light from entering the filter component 6 and the acquisition component 7. It also prevents the light emitted by the diffusion film from not entering or only entering the sample tube 100, thereby avoiding interference with the detection results. Similarly, the function of the second light-blocking plate 22 is to prevent excess excitation light from shining onto the outer wall of the sample tube 100 and entering the sample tube 100, thus making it easier to control the excitation light entering multiple sample tubes 100 to be consistent.

[0055] In some embodiments, the optical detection device further includes a first mounting plate 8, located below the light-diffusing plate 2. The first mounting plate 8 has a plurality of first grooves 81 and a plurality of second grooves 82 located below the first grooves 81, the size of the first grooves 81 being smaller than the size of the second grooves 82. A constant temperature plate 9 is disposed below the first mounting plate 8, and the constant temperature plate has a plurality of first through holes, each corresponding to a first lens 3. The first lens 3 is located between the second grooves 82 and the first through holes. This not only facilitates the installation of the first lens 3, but also prevents the sample to be tested in the sample tube 100 from forming liquefied droplets that adhere to the first lens 3 when it evaporates at high temperature, thus affecting the optical path of the excitation and emission light. At the same time, the through holes do not obstruct the excitation and emission light from entering the sample tube 100.

[0056] The size of the first groove 81 is smaller than that of the second groove 82. The first lens 3 is located between the second groove 82 and the first through hole. Therefore, the upper part of the first lens 3 is limited by the first groove 81, and the lower part of the lens is limited by the first through hole, thereby fixing the first lens 3.

[0057] The second groove 82 mentioned above is provided with an O-ring for stabilizing the first lens 3. The O-ring is arranged around the outer periphery of the first lens 3, which can further stabilize the first lens 3.

[0058] In some embodiments, the optical detection device further includes a second mounting plate 10, which is located above the light-diffusing plate 2. The second mounting plate 10 has a plurality of third grooves 101 and a plurality of fourth grooves 102 located above the third grooves 101. The size of the third grooves 101 is smaller than the size of the fourth grooves 102. A limiting plate 11 is provided above the second mounting plate 10. The limiting plate 11 has a plurality of second through holes, which are corresponding to the second lens 4. The second lens 4 is located between the fourth grooves 102 and the second through holes, which facilitates the installation of the second lens 4. Moreover, the size of the third grooves 101 is smaller than the size of the fourth grooves 102. The second lens 4 is located between the fourth grooves 102 and the second through holes. Therefore, the lower part of the second lens 4 is limited by the third grooves 101, and the upper part of the second lens 4 is limited by the second through holes, thereby fixing the second lens 4.

[0059] The aforementioned fourth groove 102 is provided with an O-ring for stabilizing the second lens 4. The O-ring surrounds the outer periphery of the second lens 4, which can further stabilize the second lens 4.

[0060] In some embodiments, when the acquisition component 7 is configured as an imaging component, such as a camera, the optical detection device further includes a first lens 12 and a first filter wheel 13 located above the first lens 12. The first filter wheel 13 includes at least one first receiving space, and a filter component 6 is located in the first receiving space. The filter component 6 is coaxially arranged with the first lens 12 and the imaging component. The emitted light passes through the first lens 12 and the filter component 6 and enters the imaging component. Finally, the imaging component captures an image of the detection result of the sample to be tested. By capturing the image, the detection results of all samples to be tested can be acquired at the same time, thereby completing the optical detection of the samples to be tested within the sample tube 100. Since the imaging component is used for the imaging detection method, the emitted light information of all samples to be tested can be acquired simultaneously, which is not only faster but also avoids the problem of detection result differences caused by the acquisition time interval. Moreover, because the components used are relatively inexpensive, the cost of the entire optical detection device is also very low.

[0061] The first filter wheel 13 is rotatable and supported by a bracket (not shown in the figure). After the first filter wheel 13 rotates, one of the filter components 6 is coaxially arranged with the first lens 12 and the imaging element. It should be noted that when there are multiple first accommodating spaces, different types of filter components 6 can be accommodated in different first accommodating spaces. For example, different types of light emission filters can be accommodated. By rotating the first filter wheel 13, different filter components 6 can be switched to meet different optical detection requirements.

[0062] In some embodiments, when the acquisition component 7 is configured as a hyperspectral imager, the optical detection device further includes a reflector 14, a second lens 15, and a second filter wheel 16 located on one side of the second lens 15. The second filter wheel 16 includes at least one second receiving space, and the filter component 6 is located in the second receiving space. The filter component 6 is coaxially arranged with the second lens 15 and the hyperspectral imager. The emitted light is reflected by the reflector 14 and enters the hyperspectral imager through the filter component 6 and the second lens 15. When the reflector 14 is rotated to a certain angle or its position is adjusted, the emitted light from one row of sample tubes 100 can be reflected to the filtering component 6 of the second filter wheel 16 for filtering. After filtering, the light enters the second lens 15, while the rest will not be reflected into the second lens 15. Finally, the full spectrum of all emitted light from one row of sample tubes 100 can be obtained by the hyperspectral analyzer. When the reflector 14 is rotated to another angle, the full spectrum of all emitted light from another row of sample tubes 100 can be obtained. By continuously changing the angle of the reflector 14, the full spectrum of all emitted light from the sample tubes 100 can be obtained, thereby achieving full spectrum detection of emitted light and making the detection results more accurate.

[0063] In some embodiments, the light source assembly 1 includes a substrate 1a, a rotating shaft 1b is disposed on the substrate 1a, a turntable 1c is rotatably connected to the rotating shaft 1b, and a plurality of laser light sources 1d are distributed on the turntable 1c. By driving the turntable 1c to rotate, different laser light sources 1d can be aligned with the light equalizing plate 2 one by one, thereby realizing the switching of different laser light sources 1d to meet different detection requirements.

[0064] Multiple turntables 1c can be set on the light source assembly 1 mentioned above, and the turntables 1c are evenly arranged on the same horizontal plane.

[0065] In some embodiments, two light source assemblies 1 are provided, and the two light source assemblies 1 are symmetrically arranged on both sides of the light homogenizing plate 2, so that the excitation light emitted by the light homogenizing plate 2 is more uniform.

[0066] Of course, multiple light source assemblies 1 can be set up. Multiple light source assemblies 1 are evenly arranged around the periphery of the light homogenizing plate 2 to ensure that the excitation light emitted by the light homogenizing plate 2 is more uniform.

[0067] In some embodiments, an excitation light filter is provided on the side of the laser light source 1d facing the light homogenizer 2 to filter the excitation light so as to obtain the light of the desired wavelength in the excitation light.

[0068] The working process of an optical inspection device:

[0069] The excitation light emitted by the laser source 1d first enters the homogenizing plate 2, and is reflected by the first reflective film 23 and the second reflective film 24 in the homogenizing plate 2. Taking the first reflective film 23 as a diffusion film and the second reflective film 24 as a brightness enhancement film as an example, the excitation light emitted from the diffusion film is collected by the first lens 3 and then enters the sample tube 100. The sample to be tested in the sample tube 100 excites emission light, which is then collected by the first lens 3, the second lens 4 and the converging lens 5 and then enters the acquisition component 7, where it is detected, thereby completing the optical detection of the sample to be tested in the sample tube 100. The excitation light reflection process in the homogenizing plate 2 is as follows: after the excitation light enters the homogenizing plate 2, the diffusion film can emit light uniformly, and the brightening film can reflect the excitation light. The light emitted by the diffusion film passes through the light inlet 241 and the second light transmission hole 221 in sequence, and then is collected by the first lens 3 and enters the sample tube 100. Then, part of the emitted light emitted by the sample to be tested in the sample tube 100 passes through the first lens 3, the second light transmission hole 221, the light inlet 241, the light receiving hole 231, the first light transmission hole 211, the second lens 4, the converging lens 5 and the filter component 6 and enters the acquisition component 7. The other part passes through the first lens 3, the second light transmission hole 221 and the light inlet 241 and enters the homogenizing plate 2. It is reflected by the first reflective film 23 and the second reflective film 24, and then passes through the light receiving hole 231, the first light transmission hole 211, the second lens 4, the converging lens 5 and the filter component 6 and enters the acquisition component 7, where it is collected and analyzed. The entire process involves homogenizing the excitation light using a homogenizing plate 2, ensuring that the excitation light reaching each sample tube 100 has nearly uniform and consistent light energy and incident angle. Furthermore, the emitted light is converged by a second lens 4 and a converging lens 5, and then filtered by a filtering component 6 to extract the desired wavelength before being collected and analyzed by the acquisition component 7. This optical detection device allows all sample tubes 100 to receive the excitation light simultaneously, and the received excitation light is nearly uniform and consistent. This not only avoids the result differences caused by time intervals in the reception of excitation light from multiple samples in the prior art, but also ensures that the excitation light reaching all sample tubes 100 is nearly uniform and consistent, thereby avoiding differences in detection results and improving the reliability of the detection results. Furthermore, the converging lens 5 and acquisition component 7 converge all emitted light for simultaneous collection and analysis, further avoiding factors that may affect the detection results, such as time intervals in the collection of emitted light from different samples. In the description of this invention, it should be understood that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing this invention and simplifying the description, and is not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0070] In the description of this specification, references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0071] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An optical detection device for a PCR instrument, characterized in that, include: A light source assembly for emitting excitation light; A light-diffusing plate is provided, with the light source assembly disposed on at least one side of the light-diffusing plate, allowing the excitation light to enter the light-diffusing plate. A sample tube is disposed below the light-diffusing plate, and the excitation light passes through the light-diffusing plate and enters the sample tube, where the sample to be tested in the sample tube emits emission light. The light-diffusing plate includes a first light-blocking plate and a second light-blocking plate located below the first light-blocking plate. The first light-blocking plate has a plurality of first light-transmitting holes, and the second light-blocking plate has a plurality of second light-transmitting holes. A first reflective film is disposed below the first light-blocking plate, and a second reflective film is disposed above the second light-blocking plate. The first reflective film has a plurality of light-receiving holes, and the second reflective film has a plurality of light-entering holes. The diameter of the first light-transmitting hole is less than or equal to the diameter of the light-receiving hole, the diameter of the light-receiving hole is less than the diameter of the second light-transmitting hole, and the diameter of the second light-transmitting hole is less than or equal to the diameter of the light-entry hole. Multiple first lenses are located between the light-diffusing plate and the sample tube, and each first lens is arranged in a one-to-one correspondence with the sample tube. Multiple second lenses are provided, with each second lens positioned above the light-diffusing plate and corresponding to a sample tube. A converging lens, located above a plurality of second lenses, is used to converge emitted light; A filtering component, located above the converging lens, is used to obtain light of the desired wavelength range in the emitted light; The light emitted passes through the filter and enters the acquisition component.

2. The optical detection device for a PCR instrument according to claim 1, characterized in that, The first lens, the first light-transmitting hole, the second light-transmitting hole, the light-receiving hole, the light-entry hole, and the second lens are coaxially arranged.

3. The optical detection device for a PCR instrument according to claim 1, characterized in that, It also includes a first mounting plate located below the light-diffusing plate. The first mounting plate has multiple first grooves and multiple second grooves located below the first grooves. The size of the first grooves is smaller than the size of the second grooves. A constant temperature plate is provided below the first mounting plate. The constant temperature plate has multiple first through holes, and each of the first through holes corresponds to a first lens. The first lens is located between the second grooves and the first through holes.

4. The optical detection device for a PCR instrument according to claim 1, characterized in that, It also includes a second mounting plate, which is located above the light-diffusing plate. The second mounting plate has multiple third slots and multiple fourth slots located above the third slots. The size of the third slots is smaller than the size of the fourth slots. A limiting plate is provided above the second mounting plate. The limiting plate has multiple second through holes, which are arranged one-to-one with the second lens. The second lens is located between the fourth slots and the second through holes.

5. The optical detection device for a PCR instrument according to claim 1, characterized in that, When the acquisition component is configured as an imaging component, the optical detection device further includes a first lens and a first filter wheel located above the first lens. The first filter wheel includes at least one first receiving space. The filter component is located in the first receiving space. The filter component is coaxially arranged with the first lens and the imaging component. The emitted light passes through the first lens and the filter component and enters the imaging component.

6. The optical detection device for a PCR instrument according to claim 1, characterized in that, When the acquisition component is configured as a hyperspectral imager, the optical detection device further includes a reflector, a second lens, and a second filter wheel located on one side of the second lens. The second filter wheel includes at least one second receiving space, and the filtering component is located in the second receiving space. The filtering component is coaxially arranged with the second lens and the hyperspectral imager. The emitted light is reflected by the reflector and enters the hyperspectral imager through the filtering component and the lens.

7. The optical detection device for a PCR instrument according to claim 1, characterized in that, The first reflective film includes a diffusion film or a brightness enhancement film, and the second reflective film includes a diffusion film or a brightness enhancement film.

8. The optical detection device for a PCR instrument according to claim 1, characterized in that, The light source assembly includes a substrate, on which a rotating shaft is disposed, and a turntable is rotatably connected to the rotating shaft, with multiple laser light sources distributed on the turntable.

9. The optical detection device for a PCR instrument according to claim 1, characterized in that, Two light source assemblies are provided, and the two light source assemblies are symmetrically arranged on both sides of the light-diffusing plate.

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