A composition with anti-aging and anti-wrinkle effects and its preparation method and application

Abstract

The present invention belongs to the field of daily chemical products and discloses a composition with anti-aging and wrinkle-removing effects, as well as its preparation method and application. The composition comprises the following components: N-acetylneuraminic acid, aminobutyric acid, inositol, niacinamide, and water. The components used in this composition are safe and reliable, have good absorbability, and exhibit excellent anti-aging, wrinkle-removing, and skin-lightening effects, promising promising applications.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemical products, and particularly relates to a composition with anti-aging and anti-wrinkle effects, and a preparation method and application thereof. Background Art

[0002] Human aging is an inevitable part of life, with skin changes being the most obvious. With age, the levels of collagen, elastin, hyaluronic acid, and glycoproteins in the skin gradually decrease, damaging the skin barrier and accelerating aging. Skin aging is primarily manifested by roughness and dullness, thickening and deepening of wrinkles, increased pigmentation, poor microcirculation, deformation of elastic tissue, thinning of the dermis, and susceptibility to allergic reactions and inflammation.

[0003] While there are a wide variety of anti-aging skincare products on the market, most are less than ideal and struggle to deliver effective anti-aging results. Medical cosmetic procedures, such as hyaluronic acid and botulinum toxin injections, laser freckle removal, and facelifts, are expensive and risky.

[0004] Therefore, the present invention hopes to provide an anti-aging product with high safety, good absorbability and significant effect, so as to better and safely play the role of skin care. Summary of the Invention

[0005] The present invention aims to address at least one of the technical problems existing in the above-mentioned prior art. To this end, the present invention provides a composition with anti-aging and wrinkle-removing effects, as well as its preparation method and use. The composition comprises safe and reliable components with good absorbability, and exhibits excellent anti-aging, wrinkle-removing, and skin-lightening effects, thus possessing promising application prospects.

[0006] The invention provides a composition with anti-aging and anti-wrinkle effects, comprising the following components: N-acetylneuraminic acid, aminobutyric acid, inositol, nicotinamide and water.

[0007] The functions of the effective components in the above composition are as follows: N-acetylneuraminic acid intervenes in cell activity, eliminates reactive oxygen species, and promotes the production of extracellular matrix and type I collagen; aminobutyric acid can regulate the excitation of nerve cells, stretch expression lines, promote the synthesis of epidermal cells and hyaluronic acid and collagen, and improve skin elasticity; inositol can increase skin water content, increase the thickness of the epidermis, repair the skin barrier, and lock in moisture; niacinamide can accelerate the metabolism of melanin keratin, brighten the skin tone, and smooth the skin.

[0008] The present invention improves the permeability and absorbability of the functional components through the rational combination of multiple functional components, and exerts a synergistic effect, making the anti-aging and wrinkle-removing effects more significant, while giving the skin an instant brightening effect.

[0009] Preferably, the composition comprises the following components in parts by weight: 0.3-6 parts of N-acetylneuraminic acid, 0.3-6 parts of aminobutyric acid, 0.3-1.8 parts of inositol, 0.5-8 parts of nicotinamide, and 50-98 parts of water.

[0010] Preferably, the composition further comprises a polyol.

[0011] More preferably, the polyol is selected from at least one of dipropylene glycol, butylene glycol, hexylene glycol, pentylene glycol and glycerol.

[0012] More preferably, the weight portion of the polyol is 20-25 parts.

[0013] The present invention also provides a method for preparing the above-mentioned composition having anti-aging and anti-wrinkle effects, comprising the following steps:

[0014] N-acetylneuraminic acid, aminobutyric acid, inositol and nicotinamide are added into water for dispersion to prepare the composition.

[0015] The present invention also provides the use of the composition with anti-aging and anti-wrinkle effects in skin care products.

[0016] The present invention also provides a skin care product comprising the above composition with anti-aging and anti-wrinkle effects.

[0017] Preferably, the skin care product further comprises at least one of a moisturizer, a preservative, an antioxidant, an emollient, and an emulsifier.

[0018] Preferably, the skin care product is selected from lotion, emulsion, cream, essence, mask or eye cream.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The components used in the composition of the present invention are all highly safe raw materials, among which N-acetylneuraminic acid can regulate and enhance cell activity and normal cell activities, and promote the production of intercellular matrix and collagen. Aminobutyric acid can promote epidermal cell proliferation, accelerate barrier repair, promote fibroblast proliferation in the dermis and the synthesis of collagen, elastin, and HA, and improve skin elasticity and firmness. The use of inositol and niacinamide helps to enhance the anti-aging and firming effect of the composition, while improving skin glossiness and giving the skin an instant brightening effect. The present invention improves the permeability and absorbability of the functional components through the rational combination of multiple functional components, and exerts a synergistic effect, making the anti-aging and wrinkle-removing effects more significant, while giving the skin an instant brightening effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1The graph shows the results of Collagen I content detection of sample 1-1, sample 1-2 and sample 1-3.

[0022] Figure 2 The graph shows the results of Collagen I content detection of sample 2-1, sample 2-2 and sample 2-3.

[0023] Figure 3 The graph shows the results of Collagen I content detection of sample 3-1, sample 3-2 and sample 3-3.

[0024] Figure 4 This is a comparative analysis chart of skin elasticity R2 of Product A and Product B.

[0025] Figure 5 This is a comparative analysis chart of skin firmness F4 of Product A and Product B.

[0026] Figure 6 This is a comparative analysis chart of the crow's feet wrinkle ratio test of Product A and Product B.

[0027] Figure 7 This is a before-and-after comparison of crow's feet using Product A and Product B.

[0028] Figure 8 This is the skin brightening effect after using Mask A.

[0029] Figure 9 This is the skin brightening effect after using Mask B. DETAILED DESCRIPTION

[0030] In order to make the technical solutions of the present invention more clearly understood by those skilled in the art, the following examples are given for illustration. It should be noted that the following examples are only preferred embodiments of the present invention and do not limit the scope of protection claimed by the present invention. Any modification, substitution, or combination made without violating the spirit and principle of the present invention is included in the scope of protection of the present invention.

[0031] Unless otherwise specified, the raw materials, reagents, or devices used in the following examples can be obtained from conventional commercial sources or by existing known methods.

[0032] Example 1: Experiment on promoting expression of type I collagen

[0033] 1. Purpose of the experiment

[0034] N-acetylneuraminic acid, aminobutyric acid, inositol and nicotinamide were used as the effective components, and the effect of the combination of the above effective components on promoting the expression of type I collagen was tested.

[0035] 2. Experimental Principle

[0036] Collagen makes up approximately 80% of the skin's dermis, plumping and filling the skin, and providing moisturizing, whitening, and soothing properties. Its content is a key indicator of skin aging. The skin is primarily composed of type I collagen and type III collagen, with type I accounting for 80%-85% and type III for 10%-15%. Type I collagen, also known as nanowires, provides a strong structural support for the skin. Loss of type I collagen leads to facial wrinkles and depressions. Therefore, this experiment measured the increase in type I collagen content to determine the anti-wrinkle and anti-aging properties of each test sample.

[0037] 3. Main Reagents

[0038] Low-glucose DMEM medium (Gibco), PBS (Solerbo), DMSO (Sigma), newborn bovine serum (NBS, Lanzhou Rongye), Collagen I ELISA kit (Abcam), N-acetylneuraminic acid (purchased from Zhongke Optics Valley), aminobutyric acid (purchased from Guangdong Le'erkang Biotechnology Co., Ltd.), inositol (purchased from Danisco (China) Investment Co., Ltd.), and nicotinamide (purchased from Guangzhou Lonza Co., Ltd.).

[0039] 4. Main equipment

[0040] CO2 incubator (Thermo, 150I), clean bench (Sujing Antai, SW-CJ-1F), microplate reader (BioTek, Epoch), inverted microscope (Olympus, CKX41).

[0041] 5. Experimental Methods

[0042] (1) Preparation of test samples: First weigh deionized water that has been sterilized at high temperature and cooled to 45-50°C and add it to a dry container. Add the remaining materials in sequence while stirring at a stirring speed of 200-500 r / min. Stir for 10-15 minutes until the mixture is evenly dispersed and transparent.

[0043] (2) Test method:

[0044] 1) Cell seeding: 2.2×10 5 Fibroblasts were seeded into 6-well plates at a seeding density of 100 cells / well and incubated overnight in an incubator (37° C., 5% CO 2 ).

[0045] 2) Dosing: When the cell plating rate in the 6-well plate reaches 40% to 60%, group dosing is performed. 2 mL of sample is added to each well, with three replicates per group. After dosing, the 6-well plate is placed in an incubator (37°C, 5% CO2) and incubated for 24 hours.

[0046] 3) Sample collection: After 24 h of culture, the cell culture supernatant was collected into an EP tube and stored in a -80°C freezer.

[0047] 4) Detection: Detection was performed according to the instruction manual of the Collagen I ELISA kit.

[0048] 5) Analysis: Graphs were generated using OriginPro, and results are expressed as mean ± SD. Comparisons between groups were analyzed using the t-test. All statistical analyses were two-tailed. P < 0.05 was considered a significant difference, and P < 0.01 was considered a highly significant difference.

[0049] The formula composition of each test sample (by mass fraction) is shown in Table 1-3.

[0050] Table 1 Formula composition of sample 1-1, sample 1-2 and sample 1-3

[0051]

[0052] Table 2 Formula composition of sample 2-1, sample 2-2 and sample 2-3

[0053]

[0054]

[0055] Table 3 Formula composition of sample 3-1, sample 3-2 and sample 3-3

[0056]

[0057] The above test samples were used to conduct corresponding tests, and the specific groups are shown in Table 4-6.

[0058] Table 4 Test groups of sample 1-1, sample 1-2 and sample 1-3

[0059]

[0060] Table 5 Test groups of sample 2-1, sample 2-2 and sample 2-3

[0061]

[0062] Table 6 Test groups of samples 3-1, 3-2 and 3-3

[0063]

[0064]

[0065] The results of the Collagen I content test of the above test samples are shown in Tables 7-9 and Figure 1-3 shown.

[0066] Table 7 Summary of Collagen I content test results for Sample 1-1, Sample 1-2, and Sample 1-3

[0067] Table 8 Summary of Collagen I content test results for Sample 2-1, Sample 2-2, and Sample 2-3

[0068]

[0069] Table 9 Summary of Collagen I content test results for samples 3-1, 3-2, and 3-3

[0070]

[0071] From Table 1-9 and Figure 1-3 It can be seen that compared with the BC group, the Collagen I content in the PC group increased significantly, indicating that the positive control in this test was effective. In addition, compared with the BC group, sample 1-1, which contained only N-acetylneuraminic acid and GABA, increased Collagen I content by 248.9%, sample 1-2, which contained only inositol and niacinamide, increased Collagen I content by 15.0%, and sample 1-3, which contained all four active ingredients (N-acetylneuraminic acid, GABA, inositol, and niacinamide), increased Collagen I content by a whopping 351.3%, significantly higher than the combined increase of samples 1-1 and 1-2 (248.9% + 15.0% = 263.9%). Similarly, the rate of increase in Collagen I content in Sample 2-3 (478.9%) was also greater than the sum of the rates of increase in Samples 2-1 and 2-2 (306.0% + 30.5% = 336.5%); and the rate of increase in Collagen I content in Sample 3-3 (587.9%) was also greater than the sum of the rates of increase in Samples 3-1 and 3-2 (383.5% + 19.45% = 402.95%). The above results show that when the four components of N-acetylneuraminic acid, aminobutyric acid, inositol, and niacinamide are compounded and used, the effect of increasing Collagen I content is more obvious at different proportions, indicating that the above components have a synergistic anti-wrinkle effect.

[0072] Example 2: Antioxidant performance test of composition samples

[0073] According to the dosage ratio of each component in Table 10, the corresponding experimental group samples were prepared using the following method:

[0074] Heat deionized water to 90°C, keep warm for 30 minutes, and then cool to 45°C. Then add the polyol and stir thoroughly. While stirring (stirring speed is 300 r / min), add N-acetylneuraminic acid, aminobutyric acid, inositol and nicotinamide in sequence until they are evenly dispersed and transparent. Filter with 800 mesh filter cloth to prepare the corresponding experimental group samples.

[0075] Table 10 Ratio of each component in the experimental group samples (by mass fraction)

[0076]

[0077] Organic free radical scavenging rate determination

[0078] 1. Experimental Principle

[0079] The DPPH· assay is a classic method for determining the in vitro antioxidant activity of drugs. In organic solvents, it is a stable free radical whose mode of action is: AH + DPPH· → DPPH-H + A·. DPPH· has strong absorption at 519nm, resulting in a deep purple color. In the presence of a free radical scavenger, DPPH·'s lone electron pairs pair, causing the color to lighten and the absorbance at the wavelength of maximum absorption to decrease. The degree of color reduction is stoichiometrically related to the number of paired electrons. Therefore, measuring absorbance at this wavelength can be used to evaluate free radical scavenging.

[0080] 2. Experimental Methods

[0081] 2.0 mL of each of the four sample solutions (experimental group 1 to experimental group 4) were accurately pipetted into test tubes, and a concentration of 2.0 × 10 -4 2.0 mL of 1.5 mol / L DPPH·ethanol solution was shaken and placed in the dark for 30 min. The absorbance at 519 nm (A i ); 2.0 mL of DPPH·ethanol solution was added with an equal volume of deionized water as a blank control sample, and its absorbance at 519 nm (A0) was measured; 2.0 mL of sample solution was added with an equal volume of deionized water as a sample control sample, and its absorbance at 519 nm (A x ) to eliminate the influence of the sample's own color.

[0082] The clearance rate was calculated as follows: R (%) = {[A0-(A i -A x )] / A0}×100%.

[0083] The experiment was repeated three times and the average clearance rate was calculated.

[0084] The test results are shown in Table 11.

[0085] Table 11 Free radical scavenging rate test results

[0086] sample Free radical scavenging rate / % Relative standard deviation RSD / % Blank control group 0 / Experimental Group 1 76.62 1.231 Experimental Group 2 92.35 1.201 Experimental Group 3 89.76 1.302 Experimental Group 4 87.65 1.128

[0087] As shown in Table 11, the free radical scavenging rates of the compositions of experimental groups 1-4 are significantly higher than those of the blank control group, indicating that they all have good free radical scavenging capabilities, and their antioxidant properties are also improved with the increase of component concentration.

[0088] Example 3: Anti-aging and firming effect test of the composition

[0089] The composition of Experimental Group 3 in Example 2 was added to the emulsion to prepare the following anti-aging and firming essence lotion. The formula composition of the anti-aging and firming essence lotion is shown in Table 12.

[0090] Table 12 Anti-aging Firming Essence Formula

[0091]

[0092]

[0093] The preparation method of the above-mentioned anti-aging firming essence lotion comprises the following steps:

[0094] (1) Glycerin, sodium hyaluronate, EMT-10, and 1,3-propylene glycol were mixed and dispersed, and then deionized water was added and heated to 90°C, and stirred until completely dissolved and uniformly dissolved, and kept warm for 30 minutes;

[0095] (2) Continue to add jojoba oil, polyglyceryl-10 myristate, and SEPIGEL 305, then homogenize and emulsify. After emulsification, stir and cool.

[0096] (3) Cooling to 40°C, adding trehalose, the composition of experimental group 3 (if not included), 1,2-hexanediol and p-hydroxyacetophenone (1,2-hexanediol and p-hydroxyacetophenone are mixed and heated to pre-dissolve until transparent), and stirring thoroughly to prepare the anti-aging firming essence lotion.

[0097] Skin elasticity improvement test and wrinkle improvement test

[0098] 1. Purpose of the experiment

[0099] By directly using samples on qualified consumers, we can confirm the product's effectiveness in improving skin elasticity and wrinkles. We apply samples and perform tests on the subjects' faces in a double-blind manner to evaluate the product's effectiveness in improving elasticity and wrinkles.

[0100] 2. Experimental Plan

[0101] Sixty subjects were randomly divided into two groups according to the experimental design. One group used Product A, and the other group used Product B. They applied the product to the face and eye area for four weeks, morning and evening after cleansing. Skin elasticity and wrinkle indicators were tracked and measured at weeks 0, 2, and 4. Skin elasticity data were collected three times on the cheeks each time, and the average was calculated. Images were obtained using an optical 3D image analyzer, and total wrinkle volume was determined by analysis.

[0102] 3. Test instruments: Cuto Dual MPA 580 (purchased from CK Company, Germany), VISIA-CR (purchased from CK Company, Germany).

[0103] The test results are shown in Tables 13-14 and Figure 4-7 shown.

[0104] Table 13 Test results using product A

[0105]

[0106] Table 14 Test results using product B

[0107]

[0108] Significance: ns indicates P>0.05, * indicates 0.01≤P≤0.05; ** indicates P<0.01.

[0109] The above test results show that there are significant differences between Product A and Product B in terms of their efficacy in improving skin elasticity, skin firmness, and crow's feet. By comparing the consumer usage test results of Product A and Product B, it can be seen that Product B (containing 5% of the composition of Experimental Group 3) has a significant effect of improving skin elasticity and improving wrinkles, indicating that the composition proposed by the present invention has excellent anti-aging and firming effects.

[0110] Example 3: Test of the instant brightening effect of the composition

[0111] The composition of Experimental Group 3 in Example 2 was applied to a facial mask to prepare the following anti-aging and skin-brightening essence mask. The formula composition of the anti-aging and skin-brightening essence mask is shown in Table 15.

[0112] Table 15 Anti-aging and brightening essence formula

[0113] Mask A Mask B Material Name Proportion% Proportion% water margin margin glycerin 3 3 1,3-Propanediol 5 5 Sodium hyaluronate 0.15 0.15 Carbomer 0.05 0.05 Arginine 0.04 0.04 Trehalose 2 2 Composition of experimental group 3 5 0 1,2-Hexanediol 0.5 0.5 p-Hydroxyacetophenone 0.5 0.5

[0114] The method for preparing the anti-aging and skin-lightening essence film comprises the following steps:

[0115] (1) Weigh glycerin, sodium hyaluronate, carbomer, and 1,3-propylene glycol, mix and disperse, then add deionized water and heat to 90°C, stir until completely dissolved and keep warm for 30 minutes;

[0116] (2) Stirring and cooling to 40°C, adding arginine, trehalose, the composition of experimental group 3 (if not available), 1,2-hexanediol and p-hydroxyacetophenone (1,2-hexanediol and p-hydroxyacetophenone were mixed and heated to pre-dissolve until transparent), and stirring thoroughly;

[0117] (3) Make a facial mask.

[0118] Instant brightening test

[0119] 1. Purpose of the experiment

[0120] By screening qualified consumers, we can directly use the test mask, take photos for comparison, and confirm the product's instant brightening effect on the skin.

[0121] 2. Experimental Plan

[0122] Ten consumers were screened and randomly divided into two groups. One group applied mask A and the other group applied mask B. According to the double-blind principle, the subjects applied the mask on their faces for 15 minutes, and photos were taken before, 10 minutes and 30 minutes after using the mask.

[0123] 3. Test results such as Figure 8-9 shown.

[0124] Depend on Figure 8-9 It can be seen that Mask A (containing 5% of the composition of Experimental Group 3) has a significant effect of brightening the skin tone, while Mask B (blank control group) has no significant effect of brightening the skin tone, indicating that the composition proposed in the present invention has an instant brightening effect.

[0125] While the embodiments of the present invention have been described in detail above with reference to the accompanying drawings, the present invention is not limited to the embodiments described above. Various modifications may be made within the scope of knowledge possessed by a person skilled in the art without departing from the spirit of the present invention. Furthermore, the embodiments of the present invention and the features thereof may be combined with one another unless there is a conflict.

Claims

1. A composition with anti-aging and anti-wrinkle effects, characterized in that: The composition is composed of the following components in parts by weight: 0.3-6 parts of N-acetylneuraminic acid, 0.3-6 parts of aminobutyric acid, 0.3-1.8 parts of inositol, 0.5-8 parts of nicotinamide, and 50-98 parts of water; Alternatively, the composition is composed of the following components in parts by weight: 0.3-6 parts of N-acetylneuraminic acid, 0.3-6 parts of aminobutyric acid, 0.3-1.8 parts of inositol, 0.5-8 parts of nicotinamide, 50-98 parts of water, and 20-25 parts of polyol.

2. The composition according to claim 1, characterized in that The polyol is selected from at least one of dipropylene glycol, butylene glycol, hexylene glycol, pentanediol and glycerol.

3. The method for preparing the composition according to claim 1 or 2, characterized in that: The following steps are involved: N-acetylneuraminic acid, aminobutyric acid, inositol and nicotinamide are added into water for dispersion to prepare the composition.

4. A skin care product, characterized in that: Comprising the composition according to claim 1 or 2.

5. The skin care product according to claim 4, characterized in that The skin care product further comprises at least one of a moisturizer, a preservative, an antioxidant, an emollient, and an emulsifier.

6. The skin care product according to claim 4, characterized in that The type of the skin care product is selected from lotion, emulsion, cream, essence, facial mask or eye cream.

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