A composition for improving anti-aging and firming effects and a preparation method thereof

Abstract

The application discloses a composition for improving anti-aging and firming effects and a preparation method thereof, and belongs to the technical field of cosmetics. The composition for improving anti-aging and firming effects comprises the following raw materials in parts by mass: galactomyces ferment l filtrate 20-60 parts, nicotinamide adenine dinucleotide 0.1-5 parts, myristyl nicotinate 0.5-5 parts, chrysanthemum coronarium leaf extract 0.2-4.5 parts, lens culinaris seed extract 0.1-5.5 parts and chrysanthemum coronarium extract 0.3-5 parts. The composition for improving anti-aging and firming effects has significant firming and anti-aging effects and is overall mild and non-irritating.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a composition that enhances anti-aging and firming effects and its preparation method. Background Technology

[0002] With changing modern lifestyles and increasing environmental pressures, skin health issues are receiving growing attention. Skin aging is a natural physiological process, but factors such as UV exposure, environmental pollution, and stress accelerate this process, leading to problems like sagging skin, wrinkles, and age spots. Furthermore, modern consumers have increasingly higher demands for the efficacy and safety of skincare products, expecting highly effective anti-aging and firming effects through scientifically formulated products.

[0003] Against this backdrop, developing a skincare product with comprehensive anti-aging and firming effects is particularly important. Summary of the Invention

[0004] The purpose of this invention is to provide a composition that enhances anti-aging and firming effects, and a method for preparing the same.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] In a first aspect, the present invention provides a composition for enhancing anti-aging and firming effects, the composition comprising the following raw materials in parts by weight:

[0007] Filtrate of galactosomal yeast fermentation products: 20-60 parts;

[0008] Nicotinamide adenine dinucleotide: 0.1-5 parts;

[0009] Myristyl nicotinate: 0.5-5 parts;

[0010] Artichoke leaf extract: 0.2-4.5 parts;

[0011] Lentil seed extract: 0.1-5.5 parts;

[0012] Golden chamomile extract: 0.3-5 parts.

[0013] This study found that the combined use of the above six ingredients has a synergistic effect, resulting in excellent firming and anti-aging effects.

[0014] As a preferred embodiment of the composition for enhancing anti-aging and firming effects according to the present invention, the composition for enhancing anti-aging and firming effects comprises the following raw materials in parts by weight:

[0015] Filtrate of galactosomal yeast fermentation products: 40-60 parts;

[0016] Nicotinamide adenine dinucleotide: 0.1-4 parts;

[0017] Myristyl nicotinate: 0.5-4 parts;

[0018] Artichoke leaf extract: 2-4.5 parts;

[0019] Lentil seed extract: 3-5.5 parts;

[0020] Golden chamomile extract: 2-5 parts.

[0021] In the most preferred embodiment of the composition for enhancing anti-aging and firming effects according to the present invention, the composition for enhancing anti-aging and firming effects comprises the following raw materials in parts by weight:

[0022] Filtrate of galactosomal yeast fermentation products: 60 portions;

[0023] Nicotinamide adenine dinucleotide: 4 parts;

[0024] Myristyl nicotinate: 4 parts;

[0025] Artichoke leaf extract: 2 parts;

[0026] Lentil seed extract: 3 parts;

[0027] Golden chamomile extract: 2 parts.

[0028] The present invention has found that the mass fraction of each raw material in a composition that enhances anti-aging and firming effects will affect the performance of the product. When the mass fraction of the raw materials is further selected within the above range (including the endpoint value), the resulting product can more significantly improve skin firmness and anti-aging effects.

[0029] Secondly, the present invention provides an essence that enhances anti-aging and firming effects, the essence containing the composition for enhancing anti-aging and firming effects described in the first aspect.

[0030] As an embodiment of the serum described in this invention, the serum comprises the following components by weight percentage:

[0031] Compositions that enhance anti-aging and firming effects: 1%-3%;

[0032] Dipropylene glycol: 50%-80%;

[0033] PEG-40 hydrogenated castor oil: 0.5%-3%;

[0034] Replenish with ultrapure water to 100%.

[0035] In a third aspect, the present invention provides a method for preparing the serum with enhanced anti-aging and firming effects as described in the second aspect, the preparation method specifically comprising the following steps:

[0036] S1: Mix 1 / 2 ultrapure water and dipropylene glycol evenly to obtain mixture A;

[0037] S2: Mix the composition that enhances anti-aging and firming effects, PEG-40 hydrogenated castor oil, and the remaining ultrapure water evenly to obtain mixture B;

[0038] S3: Mix mixture A and mixture B, homogenize for 4-6 hours to obtain an essence that enhances anti-aging and firming effects.

[0039] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0040] This invention provides a composition and its preparation method for enhancing anti-aging and firming effects. The six components in this composition work synergistically to achieve enhanced skin anti-aging and firming effects while remaining gentle and non-irritating. Specifically, the filtrate of *Galactomyces* fermentation product exhibits significant antioxidant and anti-inflammatory activity. It can inhibit oxidative stress caused by pro-inflammatory cytokines by activating the antioxidant system, thereby reducing free radical damage to skin cells and delaying skin aging. Furthermore, various bioactive components in the fermentation product can promote skin cell metabolism and regeneration, enhancing the skin's self-repair ability. Nicotinamide adenine dinucleotide (NADP) is a key factor in cellular redox processes, participating in cellular energy metabolism and DNA repair. During anti-aging, it can maintain normal cell function and slow down cellular aging caused by insufficient energy metabolism. In addition, NADP can activate the Sirtuins family protein signaling pathway, thereby slowing down cellular aging. Myristyl nicotinic acid ester is broken down into nicotinic acid in the skin, which can significantly stimulate cell regeneration and… The stratum corneum is renewed, thereby reducing wrinkles and achieving an anti-aging effect. Artichoke leaf extract is rich in active substances, some of which can effectively inhibit NF-κB activation, thus reducing inflammatory responses. Many active substances, such as polyphenols and ketones, have strong antioxidant properties, effectively scavenging free radicals and reducing skin damage caused by oxidative stress. In addition, polyphenols can promote collagen synthesis, enhancing skin elasticity and firmness. Lentil seed extract can significantly promote fibroblast activity, thereby improving skin firmness and elasticity. Golden chamomile extract contains various triterpenoids with significant anti-inflammatory effects, relieving skin sensitivity and inflammation. Furthermore, the flavonoids in golden chamomile extract have a certain inhibitory effect on melanocytes.

[0041] Therefore, nicotinamide adenine dinucleotide can delay aging through energy metabolism and DNA repair; galactosomal yeast ferment filtrate, artichoke leaf extract, and golden chamomile extract can reduce cell damage through antioxidant and anti-inflammatory effects. The four work synergistically to provide more comprehensive protection for skin cells; lentil seed extract and artichoke leaf extract can promote collagen regeneration, while myristyl nicotinate indirectly enhances collagen renewal by promoting cell regeneration. The three work synergistically to significantly improve skin firmness. These six ingredients work from multiple aspects, including antioxidant, anti-inflammatory, cell regeneration promotion, and collagen synthesis enhancement, improving the skin's anti-aging ability and firming effect through multi-target intervention. Detailed Implementation

[0042] To better understand the present invention, the present invention will be further described below with reference to specific embodiments. The terms used in "composition" and "example" are for describing specific implementation schemes and do not constitute a limitation on the scope of protection of the present invention.

[0043] Unless otherwise specified, experimental methods in the following examples are generally performed under standard conditions or as recommended by the manufacturer. Unless otherwise stated, all measurements are by percentage and parts by mass.

[0044] Some of the raw materials and their sources are as follows:

[0045] Galactosomalidia fermentation product filtrate: purchased from Guangzhou Mingtu Biotechnology Co., Ltd.

[0046] Nicotinamide adenine dinucleotide: purchased from Zhejiang Invody Biotechnology Co., Ltd.

[0047] Myristyl nicotinate: purchased from Shanghai Keqin Technology Co., Ltd.

[0048] Artichoke leaf extract: purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd., product number: L2-SCB2730.

[0049] Soldier bean seed extract: purchased from Shaanxi Mirkang Biotechnology Co., Ltd., product number: 2020060502.

[0050] Golden Chamomile Extract: Purchased from Xi'an Qiuhe Biotechnology Co., Ltd., product number: QH2024151-1.

[0051] Dipropylene glycol: purchased from Dow Chemical Company.

[0052] PEG-40 hydrogenated castor oil: purchased from BASF SE.

[0053] Unless otherwise specified, all water used in this invention is ultrapure water; other raw materials, test reagents, and cells are commercially available.

[0054] The mass fractions of each raw material in a composition for enhancing anti-aging and firming effects are shown in Table 1.

[0055] Table 1. Mass ratio of each raw material in a composition that enhances anti-aging and firming effects.

[0056]

[0057] The components and mass percentages of an essence that enhances anti-aging and firming effects are shown in Table 2.

[0058] Table 2. Components and weight percentage of a serum that enhances anti-aging and firming effects.

[0059]

[0060] The preparation methods in Examples 1-5 are as follows:

[0061] S1: Mix 1 / 2 ultrapure water and dipropylene glycol evenly to obtain mixture A;

[0062] S2: Mix the composition that enhances anti-aging and firming effects, PEG-40 hydrogenated castor oil, and the remaining ultrapure water evenly to obtain mixture B;

[0063] S3: Mix mixture A and mixture B, homogenize for 5 hours to obtain an essence that enhances anti-aging and firming effects.

[0064] Experiment 1: Firming and Anti-wrinkle Efficacy Test

[0065] Cell: Human keratinocytes (HKC).

[0066] Test substances: Compositions 1-3, Compositions ①-⑥.

[0067] Test substance concentration: Prepare a 20% (m / v) solution of the test substance using a 60 v / v ethanol aqueous solution, and then dilute it to 2% with cell culture medium at a volume ratio of 1:10. Prepare fresh before use.

[0068] Positive sample: Vitamin E.

[0069] Positive sample concentration: Dilute vitamin E to a 5% (m / v) solution using 60 v / v ethanol aqueous solution, and then dilute it to 0.05% with cell culture medium at a volume ratio of 1:100. Prepare fresh each time.

[0070] Experimental method: Refer to T / SHRH 032-2020 "Test method for firming and anti-wrinkle efficacy of cosmetics - in vitro test method for inhibition of reactive oxygen species (ROS) in keratinocytes".

[0071] See Table 3 for specific results, where X is the mean and SD is the standard deviation.

[0072] Table 3. Results of Firming and Anti-wrinkle Efficacy Tests

[0073]

[0074]

[0075] Note: "#" indicates that compared with composition 1, p < 0.05, which is significant.

[0076] As shown in Table 3, compositions 1-3 have significant firming and anti-wrinkle effects. Comparing composition 1 with compositions ①-⑤, it is evident that the galactosomal yeast fermentation product filtrate exhibits a significant synergistic effect with nicotinamide adenine dinucleotide, myristyl nicotinate, artichoke leaf extract, lentil seed extract, and golden chamomile extract. Comparing composition 1 with composition ⑥, it is clear that the synergistic effect of galactosomal yeast fermentation product filtrate, nicotinamide adenine dinucleotide, myristyl nicotinate, artichoke leaf extract, lentil seed extract, and golden chamomile extract only exists within a specific dosage range.

[0077] Experiment 2: Antioxidant Test

[0078] Preparation of DPPH solution: Dissolve 0.002g of DPPH in 50mL of 60v / v% ethanol aqueous solution to prepare a 0.1mM DPPH solution, and store it protected from light.

[0079] Sample preparation: Prepare 2wt% sample solutions of compositions 1-3 and compositions ①-⑥ using 60v / v% ethanol aqueous solution and store in the dark.

[0080] Positive control group preparation: Prepare a 0.1wt% Vc solution using a 60v / v% ethanol aqueous solution and store it protected from light.

[0081] Experimental methods: Sample groups, positive groups, blank groups, and control groups were added to different wells of a 96-well plate; among which:

[0082] Sample group: 100 μL sample solution + 100 μL DPPH solution (3 wells per concentration);

[0083] Blank group: 100 μL of sample solution + 100 μL of 60 v / v% ethanol aqueous solution (3 wells per concentration);

[0084] Positive group: 100 μL of vitamin C solution + 100 μL of 60 v / v ethanol aqueous solution (3 wells per concentration);

[0085] Control group: 100 μL DPPH solution + 100 μL 60 v / v ethanol aqueous solution.

[0086] After preparing each group of test samples, incubate them at room temperature in the dark for 30 min, and then measure the absorbance at 517 nm. Take the mean value and calculate the DPPH scavenging rate (X±SD) of each concentration of sample using the following formula, where X is the mean value and SD is the standard deviation.

[0087] Clearance rate = (1 - (A sample - A blank) / A control) * 100%;

[0088] Where A represents the absorbance of the sample group or positive group; A represents the absorbance of the blank group; and A represents the absorbance of the control group.

[0089] The results are shown in Table 4.

[0090] Table 4 Results of Antioxidant Test

[0091] experimental group Clearance rate / % (X ± SD) Composition 1 89.54±1.90％ Composition 2 86.76±4.14％ Composition 3 89.14±3.89％ Composition ① 68.29±7.45％# Composition ② 63.97±5.36％# Composition ③ 61.11±2.16％# Composition ④ 65.77±9.97％# Composition ⑤ 60.02±5.95％# Composition ⑥ 73.06±1.16％# positive sample 100％

[0092] "#" indicates that compared with composition 1, p < 0.05, which is significant.

[0093] As shown in Table 4, compositions 1-3 have significant firming and anti-wrinkle effects. Comparing composition 1 with compositions ①-⑤, it is evident that the galactosomal yeast fermentation product filtrate exhibits a significant synergistic effect with nicotinamide adenine dinucleotide, myristyl nicotinate, artichoke leaf extract, lentil seed extract, and golden chamomile extract. Comparing composition 1 with composition ⑥, it is clear that the synergistic effect of galactosomal yeast fermentation product filtrate, nicotinamide adenine dinucleotide, myristyl nicotinate, artichoke leaf extract, lentil seed extract, and golden chamomile extract only exists within a specific dosage range.

[0094] Test 3: Safety Test

[0095] The present invention experimentally verifies the biosafety of Examples 1-5.

[0096] The irritation of cosmetics was evaluated using the 2015 Cosmetic Safety Technical Specifications as a reference standard. The test method was a skin patch test, and 50 people aged 16-65 years were randomly distributed to participate in the test.

[0097] Test method: Place the test substance into a patch applicator at a dosage of 0.020-0.025 g. Cover the patch applicator containing the test substance onto the back or flexor side of the forearm of the subject with non-irritating adhesive tape. Gently press with the palm of your hand to ensure even adhesion to the skin surface. Leave on for 24 hours. Remove the patch applicator and observe the skin reaction 30 minutes after the pressure mark disappears. If the result is negative, observe again at 24 hours and 48 hours after the patch test.

[0098] Evaluation criteria:

[0099] Grade 0: Negative reaction;

[0100] Grade 1: Suspicious reaction, with only slight erythema;

[0101] Grade 2: Weak positive reaction, erythema, infiltration, edema, and possible papules;

[0102] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area;

[0103] Grade 4: Extremely positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and reaction extending beyond the test area.

[0104] Test results: All subjects had negative skin reactions.

[0105] The embodiments described above are some, but not all, embodiments of the present invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

Claims

1. A composition for enhancing anti-aging and firming effects, characterized in that, Composed of the following raw materials by weight: Filtrate of galactosomal yeast fermentation products: 20-60 parts; Nicotinamide adenine dinucleotide: 0.1-5 parts; Myristyl nicotinate: 0.5-5 parts; Artichoke leaf extract: 0.2-4.5 parts; Lentil seed extract: 0.1-5.5 parts; Golden chamomile extract: 0.3-5 parts.

2. The composition according to claim 1, characterized in that, Composed of the following raw materials by weight: Filtrate of galactosomal yeast fermentation products: 40-60 parts; Nicotinamide adenine dinucleotide: 0.1-4 parts; Myristyl nicotinate: 0.5-4 parts; Artichoke leaf extract: 2-4.5 parts; Lentil seed extract: 3-5.5 parts; Golden chamomile extract: 2-5 parts.

3. The composition according to claim 1, characterized in that, Composed of the following raw materials by weight: Filtrate of galactosomal yeast fermentation products: 60 portions; Nicotinamide adenine dinucleotide: 4 parts; Myristyl nicotinate: 4 parts; Artichoke leaf extract: 2 parts; Lentil seed extract: 3 parts; Golden chamomile extract: 2 parts.

4. A serum that enhances anti-aging and firming effects, characterized in that, A composition comprising any one of claims 1-3 that enhances anti-aging and firming effects.

5. The essence according to claim 4, characterized in that, It contains the following components by weight percentage: Compositions that enhance anti-aging and firming effects: 1%-3%; Dipropylene glycol: 50%-80%; PEG-40 hydrogenated castor oil: 0.5%-3%; Replenish with ultrapure water to 100%.

6. A method for preparing the essence as described in claim 5, characterized in that, Includes the following steps: S1: Mix 1 / 2 ultrapure water and dipropylene glycol evenly to obtain mixture A; S2: Mix the composition that enhances anti-aging and firming effects, PEG-40 hydrogenated castor oil, and the remaining ultrapure water evenly to obtain mixture B; S3: Mix mixture A and mixture B, homogenize for 4-6 hours to obtain an essence that enhances anti-aging and firming effects.

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