Multifunctional paper-based microfluidic chip based on bulk acoustic wave and preparation method and application thereof

Abstract

The application discloses a multifunctional paper-based microfluidic chip based on bulk acoustic waves and a preparation method and application thereof, and belongs to the technical field of paper-based microfluidic chips.The paper-based microfluidic chip is provided with a piezoelectric ceramic sheet and a functional area on the surface of a substrate covered by a hydrophobic material.When the amplitude of vibration of the piezoelectric ceramic sheet exceeds a certain threshold value, the vibration will cause a Faraday wave in the liquid, and the Faraday wave has a very strong mass transfer function, so that the liquid can move violently and mix fully.When the amplitude of vibration is insufficient to cause the Faraday wave, the liquid will not move violently, but the deformation of the substrate caused by the vibration will make the insoluble particles move to the nodes of the vibration, thereby generating an enrichment effect, so that the paper-based microfluidic chip has a wide application prospect in mixing, colorimetric detection and enrichment of small particles.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of paper-based microfluidic chip, and particularly relates to a multifunctional paper-based microfluidic chip based on bulk acoustic wave and a preparation method and application thereof. BACKGROUND

[0002] Since the first paper-based microfluidic chip was used for colorimetric detection in 2007, paper-based microfluidic chips have attracted great interest from researchers in various fields. Paper has many advantages, such as low cost, simple operation, disposable use and easy-to-read results, which make it a good analysis matrix, especially for point-of-care (POC) with broad application prospects.

[0003] Despite a large number of research papers, many thorny defects have not been solved and hinder the development of paper-based microfluidic. For example, the coffee ring effect causes poor uniformity of colorimetric detection on paper substrates, hindering the quantitative analysis of reagent concentration. In addition, due to its fragile nature, it is often difficult to directly perform sample processing, such as enrichment, on paper substrates. In addition, due to its non-uniform nature, it is difficult to achieve specific functions on paper, such as uniform mixing. In order to solve these problems, various strategies have been proposed. For example, in order to improve the uniformity of colorimetric detection, researchers have chosen specific types of paper or used silica nanoparticles to modify the surface of the paper. However, the specific selection compromises the universality and availability of paper, and the use of silica modification introduces excess reagents, increasing complexity and reducing reliability. In terms of sample enrichment, some researchers use electrical methods to concentrate sample ions. However, these methods need to consider the electrical properties of the sample, greatly limiting the scope of application. In order to achieve good mixing on paper, the most commonly used method is a multi-layered origami structure or a complex geometric design, such as designing a tortuous channel. However, the manufacture of these devices is more complex, requiring precise stacking of different layers or fine design of the maze path. In addition, although there are various strategies for mixing, the mixing performance of existing research cannot be called "uniform", and the edge of the end region always tends to show unmixed color. Although researchers have conducted a large amount of research on paper-based microfluidic, the complex strategies proposed are to solve a single problem in the field, and devices that achieve multiple functions through a simple strategy are very rare. SUMMARY

[0004] In order to overcome the shortcomings of the prior art described above, the purpose of the present application is to provide a multifunctional paper-based microfluidic chip based on bulk acoustic wave and a preparation method and application thereof, which solves the technical problems that existing paper-based microfluidic chips cannot achieve multifunctional applications.

[0005] In order to achieve the above purpose, the technical scheme adopted by the present application is as follows:

[0006] The application discloses a preparation method of a multifunctional paper-based microfluidic chip based on bulk acoustic waves.

[0007] The un-solidified hydrophobic material is evenly applied on the upper surface of the substrate to form a hydrophobic material covered substrate; then a template paper base is placed on one side of the upper surface of the hydrophobic material covered substrate and pressed, so that the upper surface of the hydrophobic material covered substrate forms a recess in the shape of the template paper base, to obtain a functional area; then the hydrophobic material is solidified by pretreatment, and a piezoelectric ceramic sheet is attached to the other side of the upper surface of the hydrophobic material covered substrate, and the template paper base is removed, to obtain a multifunctional paper-based microfluidic chip based on bulk acoustic waves.

[0008] Further, the substrate is a glass sheet, a silicon sheet or a PC plastic; the test paper base is placed in the functional area; and the shape of the piezoelectric ceramic sheet includes but is not limited to a circle, a square, a ring, a needle and a column.

[0009] Further, the pretreatment is performed by a method that can solidify the hydrophobic material, including but not limited to heating and drying or ultraviolet irradiation.

[0010] Further, the hydrophobic material is polydimethylsiloxane, wax or polystyrene.

[0011] Further, the shape of the template paper base includes a rectangle, a circle, a ring, a tree shape or a star shape.

[0012] The application further discloses a multifunctional paper-based microfluidic chip based on bulk acoustic waves prepared by the preparation method.

[0013] The application further discloses application of the multifunctional paper-based microfluidic chip based on bulk acoustic waves, and the multifunctional paper-based microfluidic chip based on bulk acoustic waves is used for mixing or colorimetric detection.

[0014] Further, when used for mixing or colorimetric detection, the voltage applied to the piezoelectric ceramic sheet can cause a Faraday wave caused by mass transfer of a solution.

[0015] The application further discloses application of the multifunctional paper-based microfluidic chip based on bulk acoustic waves, and the multifunctional paper-based microfluidic chip based on bulk acoustic waves is used for micro-particle enrichment.

[0016] Further, the voltage applied to the piezoelectric ceramic sheet is not more than a threshold value that can cause a Faraday wave caused by mass transfer of a solution.

[0017] Compared with the prior art, the application has the following beneficial effects:

[0018] The application discloses a preparation method of a multifunctional paper-based microfluidic chip based on bulk acoustic waves, and the chip is characterized in that a piezoelectric ceramic sheet and a functional area are arranged on a substrate surface covered by a hydrophobic material. Since additional acoustic energy is introduced, and the acoustic waves are independent of the electromagnetic and optical properties of the medium, the chip has extremely strong versatility. The chip can produce extremely strong effects without depending on the type of the paper-based substrate or introducing redundant reagents for modification, and the problems of technical complexity and limited applicability in the prior art are overcome. The preparation method is simple, direct and low in cost. Compared with the manufacturing of other paper-based microfluidic chips, for example, a paper folding method and a wax spraying method, the method does not require accurate calibration or stacking, and does not require expensive equipment and consumables such as a wax printer or photoresist.

[0019] The application further discloses applications of the multifunctional paper-based microfluidic chip based on bulk acoustic waves in mixing, colorimetric detection and micro-particle enrichment. When the piezoelectric ceramic sheet is used, if the vibration amplitude of the piezoelectric ceramic sheet exceeds a certain threshold value, the vibration can cause the liquid to generate a Faraday wave, and the Faraday wave has extremely strong mass transfer function, so that the liquid can be moved and mixed sufficiently. If the vibration amplitude is insufficient to cause the Faraday wave, the liquid will not be moved violently, but the deformation of the substrate caused by the vibration can make the insoluble particles move to the nodes of the vibration, thereby producing enrichment effect. Compared with the prior art, the application introduces a simple strategy to realize multiple functions, and the effect of each function is very significant, and the application has high flexibility and strong performance. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 FIG. 1 is a structural schematic diagram of the multifunctional paper-based microfluidic chip based on bulk acoustic waves;

[0021] Figure 2 FIG. 2 is a physical diagram of the multifunctional paper-based microfluidic chip based on bulk acoustic waves covered by polydimethylsiloxane (PDMS) and having a circular template paper-based substrate (without placing a test paper-based substrate);

[0022] Figure 3 FIG. 3 is a physical diagram of the multifunctional paper-based microfluidic chip based on bulk acoustic waves covered by polydimethylsiloxane (PDMS) and having a tree-shaped template paper-based substrate (without placing a test paper-based substrate);

[0023] Figure 4 FIG. 4 is a high-speed camera shot of the multifunctional paper-based microfluidic chip based on bulk acoustic waves; Figure 2 FIG. 5 is a Faraday wave caused by the multifunctional paper-based microfluidic chip based on bulk acoustic waves when the chip is running;

[0024] Figure 5 FIG. 6 is a high-speed camera shot of the multifunctional paper-based microfluidic chip based on bulk acoustic waves; Figure 3 FIG. 7 is a Faraday wave caused by the multifunctional paper-based microfluidic chip based on bulk acoustic waves when the chip is running;

[0025] Figure 6The experimental result graph for realizing the uniform glucose colorimetric detection function of the multifunctional paper-based microfluidic chip based on the body acoustic wave of the application is shown in the figure;

[0026] Figure 7 The experimental result graph for realizing the micro-particle (brass powder, pure copper powder, diamond powder, yeast) enrichment function of the multifunctional paper-based microfluidic chip based on the body acoustic wave of the application is shown in the figure;

[0027] Figure 8 The experimental result graph for realizing the uniform mixing function of the multifunctional paper-based microfluidic chip based on the body acoustic wave of the application is shown in the figure;

[0028] Among them: 1-hydrophobic material covered substrate; 2-piezoelectric ceramic sheet; 3-functional area; a-sound wave; b-no sound wave. DETAILED DESCRIPTION

[0029] In order to make the person in the art better understand the application scheme, the technical scheme in the embodiment of the application will be described clearly and completely in the following with the aid of the drawings in the embodiment of the application. Obviously, the described embodiment is only a part of the embodiment of the application, not all. Based on the embodiment in the application, all other embodiments obtained by the person skilled in the art without creative labor should belong to the scope of the application.

[0030] It should be noted that the terms "first", "second" and the like in the specification and claims of the application and the above-mentioned drawings are used to distinguish similar objects, and do not necessarily describe a specific order or sequence. It should be understood that the data thus used can be interchanged under appropriate circumstances, so that the embodiments of the application described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion, for example, a process, method, system, product or device including a series of steps or units does not necessarily limit to those steps or units clearly listed, but can include other steps or units not clearly listed or inherent to these processes, methods, products or devices.

[0031] The application will be described in further detail below with reference to the drawings:

[0032] As Figure 1 shown, the application discloses a multifunctional paper-based microfluidic chip based on body acoustic wave, including a hydrophobic material covered substrate 1, a functional area 3 and a piezoelectric ceramic sheet 2 are arranged on both sides of the upper surface of the hydrophobic material covered substrate 1, when running, and the test paper base with the same shape as the template paper base will be put into the pit of the functional area 3.

[0033] The hydrophobic material covering the substrate includes but is not limited to polydimethylsiloxane (PDMS), wax, polystyrene (PS) and the like; the shape of the paper base placed in the functional area includes but is not limited to rectangle, circle, ring and star.

[0034] The liquid amount of the multifunctional paper-based microfluidic chip based on bulk acoustic wave is more than the absorption amount of the paper base but less than the limit of the hydrophobic boundary; when mixing or colorimetric detection and other applications requiring solution mass transfer are performed, the voltage of the piezoelectric ceramic sheet is high enough to cause Faraday wave; when enrichment and other applications related to insoluble particles are performed, the voltage of the piezoelectric ceramic sheet is less than the threshold value of causing Faraday wave.

[0035] Figure 2 As shown in the figure, a glass substrate is covered with polydimethylsiloxane (PDMS) to form a hydrophobic material covered substrate 1, and a piezoelectric ceramic sheet 2 is arranged, and a circular recess is formed in the circular template paper base in the functional area 3, thereby forming a multifunctional paper-based microfluidic chip based on bulk acoustic wave; during operation of the chip, a test paper base with the same shape as the template paper base is placed in the recess in the functional area 3.

[0036] Figure 3 As shown in the figure, a glass substrate is covered with polydimethylsiloxane (PDMS) to form a hydrophobic material covered substrate 1, and a piezoelectric ceramic sheet 2 is arranged, and a tree-shaped recess is formed in the tree-shaped template paper base in the functional area 3, thereby forming a multifunctional paper-based microfluidic chip based on bulk acoustic wave.

[0037] Figure 4 As shown in the figure, a glass substrate is covered with polydimethylsiloxane (PDMS) to form a hydrophobic material covered substrate 1, and a piezoelectric ceramic sheet 2 is arranged, and a tree-shaped recess is formed in the tree-shaped template paper base in the functional area 3, thereby forming a multifunctional paper-based microfluidic chip based on bulk acoustic wave. Figure 2 As shown in the figure, a glass substrate is covered with polydimethylsiloxane (PDMS) to form a hydrophobic material covered substrate 1, and a piezoelectric ceramic sheet 2 is arranged, and a tree-shaped recess is formed in the tree-shaped template paper base in the functional area 3, thereby forming a multifunctional paper-based microfluidic chip based on bulk acoustic wave. Figure 4 As shown in the figure, a glass substrate is covered with polydimethylsiloxane (PDMS) to form a hydrophobic material covered substrate 1, and a piezoelectric ceramic sheet 2 is arranged, and a tree-shaped recess is formed in the tree-shaped template paper base in the functional area 3, thereby forming a multifunctional paper-based microfluidic chip based on bulk acoustic wave.

[0038] Figure 5 As shown in the figure, a glass substrate is covered with polydimethylsiloxane (PDMS) to form a hydrophobic material covered substrate 1, and a piezoelectric ceramic sheet 2 is arranged, and a tree-shaped recess is formed in the tree-shaped template paper base in the functional area 3, thereby forming a multifunctional paper-based microfluidic chip based on bulk acoustic wave. Figure 3 As shown in the figure, a glass substrate is covered with polydimethylsiloxane (PDMS) to form a hydrophobic material covered substrate 1, and a piezoelectric ceramic sheet 2 is arranged, and a tree-shaped recess is formed in the tree-shaped template paper base in the functional area 3, thereby forming a multifunctional paper-based microfluidic chip based on bulk acoustic wave.

[0039] Figure 6 As shown in the figure, the experimental results of the multifunctional paper-based microfluidic chip based on bulk acoustic wave of the present application realizing uniform glucose colorimetric detection function, since the Faraday wave has strong mass transport function, the color product of the color reaction is quickly and uniformly transported to every corner of the paper base during operation of the chip, thereby realizing the uniform colorimetric detection function, and the effect is remarkable compared with the colorimetric result without acoustic wave vibration.

[0040] Figure 7 The experimental result diagram of the present application based on the multifunctional paper-based microfluidic chip of bulk acoustic wave to realize the enrichment function of small particles (brass powder, pure copper powder, diamond powder, yeast) is shown, when the voltage of the piezoelectric ceramic sheet is less than the threshold value of generating Faraday wave, the liquid will not flow violently, but the particles sinking on the surface of the paper base will be gathered to the node of vibration, and the larger the particle size, the shorter the gathering time, and the better the effect.

[0041] Figure 8 The experimental result diagram of the present application based on the multifunctional paper-based microfluidic chip of bulk acoustic wave to realize the uniform mixing function is shown, since the Faraday wave has strong material transport function, the two kinds of liquid flowing into the chip at the inlet are uniformly mixed at the intersection, and the effect is remarkable compared with the mixing result without acoustic wave vibration.

[0042] The above content only illustrates the technical idea of the present application, and cannot limit the protection scope of the present application, any modification made according to the technical idea of the present application on the basis of the technical scheme falls within the protection scope of the claims of the present application.

Claims

1. A method for fabricating a multifunctional paper-based microfluidic chip based on bulk acoustic waves, characterized in that, Includes the following steps: Uncured hydrophobic material is evenly coated on the upper surface of the substrate to form a substrate covered with hydrophobic material (1). Subsequently, a template paper base is placed on one side of the upper surface of the substrate (1) covered with hydrophobic material and pressed, so that a pit in the shape of the template paper base is formed on the upper surface of the substrate (1) covered with hydrophobic material, and a functional area (3) is obtained; then, after pretreatment to solidify the hydrophobic material, a piezoelectric ceramic sheet (2) is attached to the other side of the upper surface of the substrate (1) covered with hydrophobic material, and the template paper base is removed to obtain a multifunctional paper-based microfluidic chip based on bulk acoustic waves.

2. The method for fabricating a multifunctional paper-based microfluidic chip based on bulk acoustic waves according to claim 1, characterized in that, The substrate is a glass sheet, a silicon wafer or PC plastic; a test paper base is placed in the functional area (3); the piezoelectric ceramic sheet is circular, square, ring-shaped, needle-shaped or columnar.

3. The method for fabricating a multifunctional paper-based microfluidic chip based on bulk acoustic waves according to claim 1, characterized in that, The pretreatment method is either heating and drying or ultraviolet light irradiation.

4. The method for fabricating a multifunctional paper-based microfluidic chip based on bulk acoustic waves according to claim 1, characterized in that, The hydrophobic material is polydimethylsiloxane, wax, or polystyrene.

5. The method for fabricating a multifunctional paper-based microfluidic chip based on bulk acoustic waves according to claim 1, characterized in that, The shape of the template paper base includes rectangle, circle, ring, tree shape or star shape.

6. A multifunctional paper-based microfluidic chip based on bulk acoustic waves, characterized in that, The multifunctional paper-based microfluidic chip based on bulk acoustic waves, as described in any one of claims 1 to 5, was prepared.

7. The application of the multifunctional paper-based microfluidic chip based on bulk acoustic waves as described in claim 6, characterized in that, The multifunctional paper-based microfluidic chip based on bulk acoustic waves is used for mixing or colorimetric detection.

8. The application of a multifunctional paper-based microfluidic chip based on bulk acoustic waves according to claim 7, characterized in that, When used for mixing or colorimetric detection, the voltage applied to the piezoelectric ceramic sheet (2) is such that the mass transfer of the solution induced a Faraday wave.

9. The application of the multifunctional paper-based microfluidic chip based on bulk acoustic waves as described in claim 6, characterized in that, The multifunctional paper-based microfluidic chip based on bulk acoustic waves is used for microparticle enrichment.

10. The application of the multifunctional paper-based microfluidic chip based on bulk acoustic waves as described in claim 9, characterized in that, When used for enriching insoluble particles, the voltage applied to the piezoelectric ceramic sheet (2) does not exceed the threshold that would cause Faraday waves to be generated by the mass transfer of the solution.

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