Automated low temperature magnetic separation and washing apparatus and method

Abstract

The application provides an automatic low-temperature magnetic separation and washing device and method, the device comprising a frame body, a sample rack, a stirring device, a magnetic adsorption module, a constant temperature cooling system, a washing system and a control module arranged on the frame body. The device can complete the low-temperature incubation, stirring, magnetic adsorption separation and waste liquid extraction of magnetic reagents in biochemical detection through automatic control, the constant temperature cooling system guarantees the whole process in a constant low-temperature environment, realizes the automatic magnetic separation and washing under the condition of low constant temperature (below 8 DEG C), can simultaneously perform magnetic separation and washing on multiple samples, and is high in efficiency; the process excludes the interference of human body temperature, and can further improve the sensitivity and accuracy of detection results.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of detection devices, in particular to an automatic low-temperature magnetic separation and washing device and method. BACKGROUND

[0002] Magnetic separation is a technology for processing materials in a magnetic field, which separates target materials by using the difference in magnetic sensitivity of elements or components and an external magnetic field. Magnetic separation technology has been widely used in biochemical detection, but it is difficult to carry out large-scale batch biological detection due to the multiple magnetic separation steps and the need for repeated washing, which consumes time and effort. In addition, the magnetic separation of some biological samples needs to be carried out in a constant low-temperature environment, and manual touching of the test tube for magnetic separation and washing operation can easily affect the sample state, causing deviations in the accuracy and sensitivity of the detection results.

[0003] The inventor has previously researched a chemical luminescence instrument magnetic bead detection pretreatment step integrated mechanism (CN111766392A), in which a grabbing and injecting integrated mechanism transfers the detection cup in the incubator to a fixed magnetic washing box through mechanical claws, and after washing and extracting waste liquid, the detection cup is transported back to the incubator while clean washing agent is injected into the detection cup by the upper injecting mechanism. Although the above mechanism can perform a certain degree of automatic magnetic separation and washing, it cannot be applied to magnetic separation processing in a low-temperature environment. SUMMARY

[0004] To solve the above problems, the present application provides an automatic low-temperature magnetic separation and washing device and method, which can reduce human influence during low-temperature magnetic separation and washing, improve detection sensitivity and accuracy, and further improve detection reliability by treating waste liquid and washing pollution of magnetic separation.

[0005] To achieve the above-mentioned purposes, the present application provides the following technical solutions:

[0006] The present application provides an automatic low-temperature magnetic separation and washing device, which comprises a frame body, a sample rack, a stirring device, a magnetic adsorption module, a constant temperature cooling system, a washing system and a control module arranged on the frame body;

[0007] The sample rack is slidably connected to the frame body and can move in the horizontal direction; the sample rack comprises one or more groups of horizontally arranged sample slots;

[0008] The stirring device comprises a stirring paddle group and a stirring paddle cleaning system; the stirring paddle group is slidably connected to the frame body and comprises a plurality of horizontally arranged stirring paddles; the stirring paddle group can move in the vertical direction; the path of vertical movement of the stirring paddle group intersects the path of horizontal movement of the sample rack, and the stirring paddle group can enter the test tube loaded in the sample rack;

[0009] The magnetic adsorption module is arranged on the horizontal movement path of the sample rack;

[0010] The constant temperature cooling system is connected with the sample holder, so that the sample holder is kept in a low temperature environment of no more than 8℃.

[0011] The washing system comprises a movable waste liquid needle, a waste liquid needle cleaning pool and a cleaning system, and the waste liquid needle can be moved to draw waste liquid from a test tube loaded on the sample holder and inject cleaning liquid.

[0012] Preferably, the constant temperature cooling system is connected with the sample holder and can move horizontally together with the sample holder.

[0013] Preferably, the constant temperature cooling system comprises a semiconductor heat sink and a heat dissipation fan, and the semiconductor heat sink is connected with the heat dissipation fan and the bottom of the sample holder respectively.

[0014] Preferably, in the stirring device, each stirring paddle can work independently.

[0015] Preferably, in the stirring device, the stirring paddle cleaning system comprises a stirring paddle cleaning pool and a first cleaning pipeline.

[0016] The stirring paddle cleaning pool is arranged vertically below the stirring paddle group and horizontally below the moving path of the sample tank.

[0017] Preferably, the cleaning system in the washing system comprises a waste liquid recovery pipeline and a second cleaning pipeline.

[0018] One end of the waste liquid recovery pipeline is connected with the waste liquid needle and the waste liquid needle cleaning pool respectively, and the waste liquid in the waste liquid needle and the waste liquid needle cleaning pool can be independently discharged from the other end of the waste liquid recovery pipeline.

[0019] One end of the second cleaning pipeline is connected with the waste liquid needle and the waste liquid needle cleaning pool respectively, and cleaning liquid can be independently injected into the waste liquid needle and the waste liquid needle cleaning pool from the other end of the second cleaning pipeline.

[0020] Preferably, the device further comprises a magnetic reagent mixing device, and the magnetic reagent mixing device comprises a magnetic reagent bottle and a rotating driving structure, and the rotating driving structure can rotate the magnetic reagent bottle to mix the magnetic reagent.

[0021] Preferably, the device further comprises a reagent filling system, and the reagent filling system comprises a movable reagent filling needle and a plurality of reagent bottles, and the movable reagent filling needle can suck reagent from the reagent bottles and fill the reagent into a sample test tube loaded on the sample holder.

[0022] Preferably, the control module comprises a sample holder horizontal movement control module, a stirring device control module, a washing system control module and a constant temperature cooling device control module.

[0023] The application also provides a method for automatic low-temperature magnetic separation and washing, which is realized by using the device.

[0024] S1, the sample tube is placed in the sample holder, the magnetic reagent is added, and the constant temperature cooling system incubates the sample tube;

[0025] S2, the sample holder is driven to move horizontally to the vertical bottom of the stirring paddle group, the stirring paddle group is lowered vertically to stir, and after the stirring is completed, the stirring paddle group is raised and the sample holder is moved horizontally to the magnetic adsorption module to perform magnetic adsorption;

[0026] The stirring paddle group is lowered vertically again, and the stirring paddle cleaning system is used for cleaning;

[0027] S3, after the magnetic adsorption is completed, the waste liquid needle is moved to the sample tube to extract the waste liquid, the waste liquid needle is moved to the waste liquid needle cleaning pool to clean the inner wall and the outer wall after the waste liquid is extracted; the above steps are repeated until the waste liquid in each sample tube is extracted;

[0028] S4, the cleaning reagent is added to the sample tube after the waste liquid is extracted, and steps S2 and S3 are repeated until the washing is completed.

[0029] Compared with the prior art, the application has the following advantages:

[0030] 1、The automatic low-temperature magnetic separation and washing device provided by the application can complete the functions of magnetic reagent low-temperature incubation, stirring, magnetic adsorption separation and waste liquid extraction in biochemical detection through full-automatic control, and the constant temperature cooling system ensures that the whole process is in a continuous and constant low-temperature environment, realizes automatic magnetic separation and washing under low-temperature constant temperature (below 8℃), and can simultaneously perform magnetic separation and washing on multiple samples, which is high in efficiency; the process excludes manual body temperature interference, and can further improve the sensitivity and accuracy of the detection result.

[0031] 2、The automatic low-temperature magnetic separation and washing device provided by the application has a reasonable structure, the sample holder can move horizontally, the magnetic adsorption module, the stirring device and the washing system are distributed on the horizontal movement path of the sample holder, which effectively reduces the use of mechanical arms in the magnetic separation and washing process, multiple sample tubes can be simultaneously subjected to magnetic separation, stirring and other operations, which is higher in efficiency; in addition, the device avoids the use of mechanical arms to grab and move the sample tubes, reduces the risk of sample tube falling off, not only reduces the control difficulty of the whole automatic biochemical detector and saves space, but also reduces the risk of sample tube falling off caused by the movement of the mechanical arms and improves the detection accuracy.

[0032] 3、The automatic low-temperature magnetic separation and washing device provided by the application comprises a plurality of liftable stirring paddles and a cleaning system of the stirring paddles, the stirring and mixing are more efficient than conventional devices, cross contamination is prevented through the cleaning system, and the magnetic separation efficiency is further improved.

[0033] 4、The automatic low-temperature separation and washing device provided by the application, wherein the washing system comprises a movable waste liquid needle, a waste liquid needle cleaning pool and a cleaning system, the waste liquid needle can be cleaned by the cleaning system in the waste liquid pool, the waste liquid needle can be completely cleaned after each time of waste liquid extraction, cross contamination is prevented, and the device is more convenient and fast.

[0034] 5、In some preferred schemes of the application, the magnetic reagent mixing device is further included, the device can continuously rotate the reagent bottle loaded with the magnetic reagent, the stratification or uneven phenomenon caused by the weight of the magnetic reagent is avoided, the concentration of the magnetic reagent is more accurate when the magnetic reagent is taken, and the detection accuracy is improved. BRIEF DESCRIPTION OF DRAWINGS

[0035] Figure 1 It is a schematic diagram of the overall structure of the automatic low-temperature magnetic separation and washing device described in the application.

[0036] Figure 2 It is an exploded view of the automatic low-temperature magnetic separation and washing device described in the application.

[0037] Figure 3 It is a schematic diagram of the structure distribution of the automatic low-temperature magnetic separation and washing device described in the application without a sample tank.

[0038] Figure 4 It is a schematic diagram of the structure of the magnetic reagent mixing device described in the application.

[0039] In the drawings, 100 is a frame body, 200 is a sample frame, 300 is a stirring device, 400 is a magnetic adsorption module, 500 is a constant-temperature cooling system, 600 is a washing system, 110 is a side frame body, 120 is a bottom plate, 210 is a sample tank, 220 is a horizontal sliding rail, 230 is a horizontal driving motor, 310 is a stirring paddle group, 311 is a stirring paddle motor, 312 is a stirring paddle, 320 is a vertical driving motor, 330 is a stirring paddle cleaning pool, 410 is a magnet, 510 is a semiconductor heat sink, 520 is a cooling fan, 610 is a waste liquid needle, 620 is a waste liquid needle cleaning pool, 630 is a first waste liquid needle motor, 640 is a second waste liquid needle motor, 710 is a magnetic reagent bottle, 721 is a rotating driving motor, and 722 is a belt transmission mechanism. DETAILED DESCRIPTION

[0040] The technical solutions provided by the application will be described in detail below in combination with embodiments, but they cannot be understood as limitations on the protection scope of the application.

[0041] In the description of the present application, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "head", "tail", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application.

[0042] Figure 1 、 2 The specific structure of the automatic low-temperature magnetic separation and washing device of the present application is shown, which comprises a frame body 100, the frame body 100 comprises a side frame body 110 and a bottom plate 120 perpendicular to each other, a sample rack 200, a stirring device 300, a magnetic adsorption module 400, a constant temperature cooling system 500, a washing system 600 and a control module are installed on the frame body; the frame body 100 can be detachably connected with the above-mentioned structure, or can be integrally formed. In the present application, the frame body 100 is used to carry and connect various structures, the sample rack 200 can load sample test tubes and move in the horizontal direction as needed, the stirring device 300 can stir and mix the substances in the sample test tubes, the magnetic adsorption module 400 is used to provide an external magnetic field during magnetic separation, the constant temperature cooling system 500 is used to maintain a low-temperature environment for the sample test tubes during magnetic separation and washing in the device, the washing system 600 is used to remove waste liquid and inject cleaning liquid during magnetic separation and washing of the sample test tubes, and the control module is used to control the automatic operation of various structures. The mutual cooperation between the structures of the present application can realize rapid, efficient and automatic multi-sample magnetic separation and washing operation, and the low-temperature environment can be maintained throughout the processing process, thereby improving the detection accuracy. In some preferred embodiments of the present application, the automatic low-temperature magnetic separation and washing device of the present application can further comprise a magnetic reagent mixing device and / or a reagent filling system; the magnetic reagent mixing device 700 is used to carry magnetic reagents and maintain uniform concentration, thereby improving the accuracy of magnetic reagent filling and further improving the detection accuracy; the reagent filling system can realize automatic reagent filling, thereby improving the multi-sample detection effect and further reducing the error of manual operation.

[0043] As Figure 1 、 2As shown, the sample rack 200 of the present invention is slidably connected to the base plate 120 of the frame via a horizontal slide rail 220, and the sample rack 200 is moved horizontally on the horizontal slide rail 220 by a horizontal drive motor 230. The sample rack 200 consists of a set of eight horizontally arranged sample slots, each of which can accommodate one sample tube. Those skilled in the art should understand that the accompanying drawings of the present invention are merely exemplary structures. The horizontal slide rail 220 of the present invention can also be set on the side frame 110; the sample slots 210 of the sample rack 200 can be one or more rows, such as two, three, or four rows; the number of sample slots in each row of the sample rack 200 can be any integer number, such as 2, 3, 5, 10, or 12; when there are multiple rows of sample slots 210, the horizontal slide rail 220 and the horizontal drive motor 230 can also be one or more.

[0044] like Figure 1 , 2 As shown in Figure 3, the constant temperature cooling system 500 of the present invention includes a semiconductor heat sink 510 and a cooling fan 520. Both the cooling fan 520 and the sample holder 200 are mounted on the semiconductor heat sink 510, which is mounted on a horizontal slide rail 220. Those skilled in the art should understand that the accompanying drawings of the present invention are merely exemplary structures. The constant temperature cooling system 500 and / or the sample holder 200 of the present invention can also be directly connected to the frame; the semiconductor heat sink 510 can also be located on the side of the sample holder, cover the sample holder, or be entirely or partially composed of the semiconductor heat sink 510; the semiconductor heat sink 510 can be made of a material with high specific heat capacity and good thermal conductivity, such as aluminum alloy, which can effectively transfer heat between the sample holder and the cooling fan 520; the cooling fan 520 can be one or more, for example, one, two, four, etc. The constant temperature cooling system 500 described in this invention can maintain a low temperature environment below 8°C within the sample holder (for example, in cell detection, the ambient temperature often needs to be controlled at 2-8°C). The specific temperature of the low temperature environment can be achieved by adjusting the speed and on / off frequency of the cooling fan 520. The constant temperature cooling system described in this invention can achieve a continuous and stable specified low temperature environment in no more than 30 minutes.

[0045] like Figure 1 , 2As shown in FIGS. 1, 2 and 3, the magnetic adsorption module 400 of the present application comprises a plurality of magnets 410, the magnetic adsorption module 400 is arranged on the side frame body 110 and located on a side wall that can be passed through when the sample rack 200 moves horizontally, and the sample rack 200 can be moved to the position where the magnetic adsorption module 400 is arranged when external magnetic field is needed for magnetic separation. It should be understood by those skilled in the art that the structure in the drawings of the present application is only exemplary. The magnets in the magnetic adsorption module 400 of the present application can also be replaced by a magnetic field generating device, and the magnetic adsorption module 400 can also simultaneously comprise a magnet and a magnetic field generating device; those skilled in the art can also adjust the position of the magnetic adsorption module 400 according to actual needs, for example, placed at the bottom of the horizontal movement path of the sample rack 200, the magnetic adsorption module 400 of the present application is arranged on one side of the horizontal movement path of the sample rack 200 in order to make the substances collected by magnetic separation adhere to the side wall of the sample tube, and facilitate the subsequent recovery of waste liquid.

[0046] As Figure 1 , 2As shown, the stirring device 300 of the present application comprises a stirring paddle group, a vertical driving motor 320, a vertical slide rail 340 and a stirring paddle cleaning system comprising a stirring paddle cleaning pool 330 and a first cleaning pipeline; the stirring paddle group comprises 8 stirring paddles 312 arranged horizontally in equal number to the sample slots 210, each of which is provided with a stirring paddle motor 311 so that the opening and closing, rotation speed, direction, etc. of each stirring paddle can be controlled individually; the vertical slide rail 340 is arranged vertically on the side frame body, and the vertical driving motor 320 can drive the stirring paddle group to move up and down along the vertical slide rail 340, the path of vertical movement of the stirring paddle group intersecting the path of horizontal movement of the sample holder; the stirring paddles 312 in the stirring paddle group move vertically downward, and when the sample holder moves to the position below the stirring paddle group, the opening of the corresponding sample slot 210 is directly below the stirring paddles 312, the bottom of the sample slot 210 is provided with the stirring paddle cleaning pool 330, and after the sample holder 200 is removed, the stirring paddle group can be further moved downward to the stirring paddle cleaning pool 330 for cleaning the stirring paddles. In the present application, the stirring paddle cleaning pool 330 is provided with a plurality of stirring paddle cleaning grooves corresponding to the positions and number of the stirring paddles, so as to independently clean each stirring paddle and prevent cross contamination; the stirring paddle cleaning pool 330 is usually arranged on the bottom plate 120. In the present application, the first cleaning pipeline has the functions of conveying liquid into the stirring paddle cleaning pool and discharging waste liquid after cleaning the stirring paddles; the first cleaning pipeline comprises a first liquid inlet pipe and a first liquid outlet pipe, one end of the first liquid inlet pipe is connected with the stirring paddle cleaning pool, the stirring paddle cleaning liquid enters and is conveyed into the stirring paddle cleaning pool through the other end of the first liquid inlet pipe, and one end of the first liquid outlet pipe is connected with the stirring paddle cleaning pool, and the waste liquid after cleaning the stirring paddles is discharged from the other end of the first liquid outlet pipe. Those skilled in the art should understand that the structure in the drawings of the present application is only exemplary. The stirring paddle group of the present application can comprise one or more groups of horizontally arranged stirring paddles 312, for example, one group, two groups, three groups or five groups, which can be the same as or different from the number of horizontally arranged groups of sample slots; the stirring paddle motor 311 can be arranged at other positions such as the side or the bottom in addition to the top end.

[0047] As Figure 1 , 2As shown, the washing system 600 comprises a movable waste needle 610, a waste needle cleaning pool 620, a waste needle first motor 630, a waste needle second motor 640, and a cleaning system comprising a waste recovery pipeline and a second cleaning pipeline; the waste needle 610 is hollow, liquid enters and exits from the waste needle; the waste needle first motor 630 can drive the waste needle to move in the vertical direction, the waste needle second motor 640 can drive the waste needle to move in the horizontal direction, the waste needle first motor 630 and the waste needle second motor 640 cooperate to make the waste needle move in a vertical plane intersecting with the horizontal movement path of the sample holder, the waste needle cleaning pool 620 is arranged at a position of the plane, and the waste needle can be moved to the waste needle cleaning pool 620 for internal and external cleaning; one end of the waste recovery pipeline is connected with the waste needle 610 and the waste needle cleaning pool 620 respectively, and the waste liquid in the waste needle 610 and the waste needle cleaning pool 620 can be independently discharged from the other end of the waste recovery pipeline; one end of the second cleaning pipeline is connected with the waste needle 610 and the waste needle cleaning pool 620 respectively, and the cleaning liquid can be independently injected into the waste needle 620 and the waste needle cleaning pool 620 from the other end of the second cleaning pipeline. Those skilled in the art should understand that the structure in the drawings of the present application is only exemplary. The waste needle cleaning pool 620 described in the present application can be one or more, for example, two, three, five, etc.; the waste needle first motor 630 and the waste needle second motor 640 described can also be combined into one motor or other braking structure.

[0048] In some preferred embodiments of the present application, the device described in the present application further comprises a magnetic reagent mixing device, such as Figure 4 As shown, the magnetic reagent mixing device comprises a magnetic reagent bottle 710 and a rotating driving structure, which can make the magnetic reagent bottle rotate to mix the magnetic reagent. In a specific embodiment, the rotating driving structure comprises a rotating driving motor 721 and a belt driving structure 722, which are respectively connected with the rotating motor 721 and the bottom of the magnetic reagent bottle 710, so as to make the magnetic reagent bottle 710 rotate and mix. In the present application, the magnetic reagent comprises but is not limited to a reagent containing magnetic beads. In the present application, the magnetic reagent mixing device can be independently arranged outside the rack body 100, or can be arranged above the rack body 100 for convenient use.

[0049] In some preferred embodiments of the present application, the device described in the present application further comprises a reagent filling system, which comprises a movable reagent filling needle and a plurality of reagent bottles, and the movable reagent filling needle can suck the reagent from the reagent bottles and fill it into the sample test tube loaded on the sample holder. In a specific embodiment of the present application, the reagent filling system is similar to the structure of the washing system; in another specific embodiment of the present application, the reagent filling needle is moved by a mechanical arm in the reagent filling system, which expands the arrangement position possibility of the reagent bottles and / or the magnetic reagent mixing device.

[0050] The control module of the present application preferably comprises a sample rack horizontal movement control module, a stirring device control module, a washing system control module and a constant temperature cooling device control module. The control module of the present application can control the device to complete the automatic low-temperature magnetic separation washing described in the following method:

[0051] A method of automatic low-temperature magnetic separation washing, comprising the following steps:

[0052] S1, a plurality of sample tubes containing samples to be tested are placed in the sample rack 200, magnetic reagents are added according to the requirements of the detection method, and the constant temperature cooling system cools the sample tubes according to the pre-set cooling temperature, and the samples to be tested are incubated at low temperature for a period of time;

[0053] S2, after the low-temperature incubation is completed, the sample rack 200 is controlled to move horizontally along the horizontal slide rail 220 to be directly below the stirring paddle group, the stirring paddle group is lowered vertically, the parameters of each stirring paddle are set as needed and stirring is started, after completion, the stirring paddle group is raised and the sample rack 200 is moved horizontally to the magnetic adsorption module 400 for magnetic adsorption, and the magnetic separation is completed;

[0054] The stirring paddle group after stirring is lowered vertically again to the lower stirring paddle cleaning pool 330, and cleaning is performed by using the stirring paddle cleaning system;

[0055] S3, after the magnetic adsorption is completed, the waste liquid needle is moved to the sample tube to extract the waste liquid, and the waste liquid needle after extracting the waste liquid is moved to the waste liquid needle cleaning pool for inner wall and outer wall cleaning; the above steps are repeated until the waste liquid in each sample tube is extracted.

[0056] S4, washing reagents are added to the sample tube after extracting the waste liquid, and steps S2 and S3 are repeated until the washing is completed.

[0057] In the present application, if the detection method does not require washing or redissolving the sample after magnetic adsorption, step S4 is omitted. In some specific embodiments of the present application, step S4 adds washing reagents to the sample tube through the waste liquid needle, and at this time the waste liquid needle is also connected with a sample cleaning pipeline.

[0058] The above only describes the preferred embodiments of the present application, and it should be noted that for those skilled in the art, without departing from the principles of the present application, a number of improvements and refinements can be made, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. An automated low temperature magnetic separation washing device, characterized in that, The device comprises a frame, a sample rack, a stirring device, a magnetic adsorption module, a constant temperature cooling system, a washing system and a control module arranged on the frame; The sample rack is slidably connected with the frame and can move in the horizontal direction; the sample rack comprises one or more groups of sample slots arranged horizontally; The stirring device comprises a stirring paddle group and a stirring paddle cleaning system; the stirring paddle group is slidably connected with the frame and comprises a plurality of stirring paddles arranged horizontally; the stirring paddle group can move in the vertical direction; the path of the stirring paddle group moving in the vertical direction intersects with the path of the sample rack moving in the horizontal direction; the stirring paddle group can enter the test tube loaded on the sample rack; the stirring paddle cleaning system comprises a stirring paddle cleaning pool and a first cleaning pipeline; The stirring paddle cleaning pool is arranged vertically below the stirring paddle group and below the path of the sample slots moving in the horizontal direction; The magnetic adsorption module is arranged on the path of the sample rack moving in the horizontal direction; The constant temperature cooling system is connected with the sample rack to maintain a low temperature environment of not more than 8℃ for the sample rack; The washing system comprises a movable waste liquid needle, a waste liquid needle cleaning pool and a cleaning system; the waste liquid needle can move into the test tube loaded on the sample rack to extract waste liquid and inject cleaning liquid.

2. The automated low-temperature magnetic separation washing device according to claim 1, characterized in that, The constant temperature cooling system is connected with the sample rack and can move in the horizontal direction together with the sample rack.

3. The automated low-temperature magnetic separation washing device according to claim 1 or 2, characterized in that, The constant temperature cooling system comprises a semiconductor heat sink and a heat dissipation fan; the semiconductor heat sink is connected with the heat dissipation fan and the bottom of the sample rack respectively.

4. The automated low-temperature magnetic separation washing device according to claim 1, characterized in that, The cleaning system in the washing system comprises a waste liquid recovery pipeline and a second cleaning pipeline; One end of the waste liquid recovery pipeline is connected with the waste liquid needle and the waste liquid needle cleaning pool respectively; the waste liquid in the waste liquid needle and the waste liquid needle cleaning pool can be independently discharged from the other end of the waste liquid recovery pipeline; One end of the second cleaning pipeline is connected with the waste liquid needle and the waste liquid needle cleaning pool respectively; cleaning liquid can be independently injected into the waste liquid needle and the waste liquid needle cleaning pool from the other end of the second cleaning pipeline.

5. The automated low-temperature magnetic separation washing device of claim 1, wherein, The device further comprises a magnetic reagent mixing device; the magnetic reagent mixing device comprises a magnetic reagent bottle and a rotating driving structure; the rotating driving structure can rotate the magnetic reagent bottle to mix the magnetic reagent.

6. The automated low-temperature magnetic separation washing device according to claim 1 or 5, characterized in that, The device further comprises a reagent filling system; the reagent filling system comprises a movable reagent filling needle and a plurality of reagent bottles; the movable reagent filling needle can suck reagent from the reagent bottles and fill the reagent into the sample test tube loaded on the sample rack.

7. The automated low-temperature magnetic separation washing device of claim 1, wherein, The control module comprises a sample rack horizontal movement control module, a stirring device control module, a washing system control module and a constant temperature cooling device control module.

8. A method of automated low temperature magnetic separation washing, characterized in that, The device is realized by using any one of claims 1-7 and comprises the following steps: S1, placing a sample test tube into the sample rack, adding a magnetic reagent and incubating the sample test tube by the constant temperature cooling system; S2, driving the sample rack to move horizontally to the vertical bottom of the stirring paddle group, vertically lowering the stirring paddle group to stir, lifting the stirring paddle group after the stirring is completed and moving the sample rack horizontally to the magnetic adsorption module to perform magnetic adsorption; The stirring paddle group is vertically lowered again to perform cleaning by using the stirring paddle cleaning system; S3, after the magnetic adsorption is completed, the waste liquid needle is controlled to move to the sample test tube to extract the waste liquid, and the waste liquid needle moves to the waste liquid needle cleaning tank for inner wall and outer wall cleaning; the above steps are repeated until the waste liquid in each sample test tube is extracted; S4, adding a cleaning reagent to the sample test tube after the waste liquid is extracted, repeating steps S2 and S3 until the washing is completed.

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