A kind of insecticidal composition containing fluorine oxazole amide

Abstract

The present invention provides a fluorine-containing oxazolidinone insecticide composition, the insecticide composition comprises a first active ingredient, fluorine oxazolidinone, a second active ingredient, fluorine benzoate, wherein the mass ratio of the first active ingredient to the second active ingredient is 20:1-1:50; the mass percentage of the sum of the weight of the first active ingredient and the second active ingredient in the insecticide composition to the total weight of the insecticide composition is 1% to 90%, and the dosage form is a suspension, a water-dispersible granule, a dispersible oil suspension, a wettable powder, an aqueous emulsion, a microemulsion, a suspoemulsion, etc. The insecticide composition has a certain synergistic effect on agricultural pests such as yellow striped flea beetles, whiteflies, thrips, etc., and in a specific agricultural practice process, it can effectively delay the generation of pest resistance, reduce the amount of medicine used, reduce costs, and protect the environment.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide compounding, and in particular relates to an insecticide composition containing fluoxetine. Background Art

[0002] Fluxametamide, English name: fluxametamide, is an isoxazole insecticide developed by Nissan Chemical Co., Ltd. Its mechanism of action is a γ-aminobutyric acid (GABA) gated chloride channel allosteric modulator. It is mainly used in crops such as vegetables, fruit trees, cotton, and tea trees to control pests and mites such as thrips, whiteflies, leaf miners, beetles, red spiders, and rust mites.

[0003] Flunicamid, English name: flonicamid, is a pyridineamide insecticide discovered by Ishihara Sangyo Co., Ltd. of Japan. Flubendiamide is a highly effective and selective insecticide with good systemic absorption, strong penetration and long lasting effect. It can be used for fruit trees, cereals, potatoes, rice, cotton, vegetables, beans, melons, eggplants, tea trees, stone fruits, sunflowers, tomatoes and greenhouse ornamental plants to control piercing-sucking mouthparts pests such as cotton aphid (Aphis gossypii), potato aphid (Aphis nasturtii), whitefly (Bemisia spp.), plantain aphid (Dysahis plantaginea), false-eyed green leafhopper (Empoaska onukii), peach aphid (Myzus persicae), brown planthopper (Nilaparvata lugens), small yellow thrips (Scirtothrips dorsalis), wheat aphid (Sitobion avenae), thrips and greenhouse whitefly (Trialeurodes vaporariorum). Among them, it has excellent control effect on aphids and shows high toxicity.

[0004] In the process of chemical pesticide control, long-term use of a single agent can easily cause pests to develop resistance. If the dosage is increased, it is easy to cause a vicious cycle of higher resistance and greater dosage. Therefore, compound agents are an effective way to solve the problem of resistance. Compound agents that can produce synergistic effects can not only delay the development of pest resistance, but also improve prevention effectiveness, reduce dosage, and reduce costs. Summary of the invention

[0005] The object of the present invention is to provide an insecticidal composition containing fluoxetine for preventing and controlling agricultural pests.

[0006] To achieve the above object, the technical solution of the present invention is as follows:

[0007] An insecticidal composition containing fluoxetine amide, characterized in that the composition contains two active ingredients, the first active ingredient is fluoxetine amide, and the second active ingredient is fluphenazine amide;

[0008] The weight ratio of the first active ingredient to the second active ingredient is 20:1-1:50.

[0009] The insecticidal composition containing fluoxetine amide of the present invention can be formulated into various dosage forms permitted in agriculture according to methods known to those skilled in the art.

[0010] The insecticidal composition containing fluoxetine amide of the present invention can be prepared into various dosage forms for agricultural application. In addition to effective active ingredients, it also includes various auxiliary ingredients that are allowed and acceptable in different pesticide dosage forms.

[0011] The auxiliary ingredients include, but are not limited to, emulsifiers, dispersants, wetting agents, solvents, antifreeze agents, defoamers, thickeners, disintegrants, synergists, preservatives and fillers, etc., which are all commonly used or permitted ingredients in pesticide formulations and are not particularly limited. The specific ingredients and amounts are determined through experiments based on the formulation requirements.

[0012] The emulsifier may be any emulsifier known in the field of pesticide formulations, and may be one or more of phenylethylphenol polyoxyethylene polyoxypropylene ether, alkylaryl polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ether, fatty alcohol polyoxyethylene ether, glycerol laurate polyoxyethylene ether, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol polyoxypropylene polyoxyethylene ether, styrylphenyl polyoxyethylene ether, alkylphenol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, fatty alcohol polyoxyethylene ether sulfonate, alkylphenol polyoxyethylene formaldehyde condensate, calcium dodecyl sulfonate, alkylphenol polyoxyethylene ether succinic acid monoester sulfonate, alkylphenol polyoxyethylene phosphate, and alkylphenol polyoxyethylene ether sulfonated succinate.

[0013] The dispersant may be any of various dispersants known in the field of pesticide formulations, and may be one or more of alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether sulfate, phenethylphenol polyoxyethylene ether phosphate, polyoxyethylene polyoxypropylene ether block copolymer, sodium lignin sulfonate, sodium methylene dinaphthalene sulfonate (dispersant NNO), calcium lignin sulfonate, polycarboxylate, sodium methylene bisnaphthalene sulfonate, sodium methylene bismethylnaphthalene sulfonate, polyvinyl pyrrolidone, alkylamido taurate, sodium salt of alkylnaphthalene sulfonic acid polycondensate, and sodium methylene bisnaphthalene sulfonate formaldehyde condensate.

[0014] The wetting agent can be various wetting agents known in the field of pesticide formulations, and can be one or more of sodium alkylnaphthalene sulfonate, sodium dodecyl sulfate, sodium secondary alkyl sulfate, sodium dodecylbenzene sulfonate, fatty alcohol polyglycol ether sulfate, fatty alcohol polyoxyethylene ether, nonylphenol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, alkylnaphthalene formaldehyde condensate, alkylnaphthalene sulfonate and other wetting agents.

[0015] The solvent may be any solvent known in the field of pesticide formulations, and may be one or more of water, methanol, ethanol, N-methylpyrrolidone, N,N-dimethylformamide, N,N-dimethylacetamide, tetrahydrofurfuryl alcohol, toluene, xylene, dimethyl sulfoxide, tetrahydrofuran, cyclohexanone, n-butanol, isopropanol, solvent oil No. 100, No. 150, No. 180, and No. 200.

[0016] The antifreeze agent may be any antifreeze agent known in the field of pesticide formulations, and the antifreeze agent may be one or more of ethylene glycol, propylene glycol, glycerol, diethylene glycol, urea, and inorganic salts.

[0017] The defoamer may be any defoamer known in the field of pesticide formulations, and may be one or more of silicone compounds, epoxy soybean oil, emulsified silicone oil, and fatty alcohol.

[0018] The thickener may be any thickener known in the field of pesticide formulations, and may be one or more of xanthan gum, polyvinyl alcohol, sodium alginate, gum arabic, and magnesium aluminum silicate.

[0019] The disintegrant may be any disintegrant known in the field of pesticide formulations, and may be one or more of ammonium sulfate, sodium carboxymethyl starch, urea, low-substituted hydroxypropyl cellulose, cross-linked sodium carboxymethyl cellulose, cross-linked polyvinylpyrrolidone, sodium alginate, sodium bicarbonate, and sodium chloride.

[0020] The carrier can be various carriers known in the field of pesticide formulation, and can be one or more of glucose, white carbon black, kaolin, diatomaceous earth, light calcium carbonate, heavy calcium carbonate, attapulgite, soluble starch, urea, maltose, sucrose, citric acid, potassium carbonate, sodium carbonate, and anhydrous sodium sulfate.

[0021] The preservative may be any preservative known in the field of pesticide formulations, and the preservative may be one or more of kasone, sodium benzoate, benzoic acid, potassium sorbate, and formaldehyde.

[0022] The deionized water is industrial deionized water.

[0023] The beneficial effects achieved by the present invention are:

[0024] 1. The present invention discloses an insecticidal composition containing fluorine-containing oxazole amide, wherein the two effective active ingredients have different action mechanisms and can effectively delay the development of insecticide resistance after compounding.

[0025] 2. The insecticidal composition containing fluorine oxazole amide of the present invention can expand the insecticidal spectrum after the two active ingredients are compounded.

[0026] 3. The insecticidal composition containing fluorine oxazolidinone of the present invention has a synergistic effect after the two active ingredients are compounded, which can improve the prevention effect, reduce the amount of pesticide used, and reduce the cost. DETAILED DESCRIPTION

[0027] The combined effect of the mixture of fluoxetine amide and flubendiamide is illustrated below through indoor toxicity assay.

[0028] 1. Determination of the combined effects of fluoxetine and flonicamid on the yellow flea beetle

[0029] The test method uses the leaf immersion method. First, the original drug organic solvent is prepared into 5 series of concentrations of liquid for use, and each concentration is repeated 4 times. Select green vegetable leaves of the same size in the field, then wash them with clean water, wrap the base with absorbent cotton to keep them moist, immerse them in the liquid for 10 seconds, dry them, and put them into wide-mouth bottles, with 2 leaves in each bottle. Inoculate 20 healthy yellow striped flea beetles with consistent growth status into each bottle and seal them with non-woven fabric. Finally, place them in a constant temperature incubator with a temperature of 26±1℃, a relative humidity of 60-70%, and a light L:D of 16h:8h for cultivation. After 24 days, check the number of dead insects in each treatment, and calculate the post-treatment effect-corrected mortality rate (control mortality rate <5%, no correction is required; control mortality rate is between 5% and 20%, correction is required; control mortality rate >20%, the test needs to be repeated).

[0030] Mortality rate (%) = number of dead insects / total number of insects treated × 100

[0031] Corrected mortality rate (%) = (treatment mortality rate - blank control mortality rate) / (1 - blank control mortality rate) × 100

[0032] The co-toxicity coefficient method is adopted to evaluate the combined effect of the fluorine-containing oxazolidinone insecticide composition of the present invention on the yellow striped flea beetle. A co-toxicity coefficient CTC>120 indicates a synergistic effect, a co-toxicity coefficient between 80≤CTC≤120 indicates an additive effect, and a co-toxicity coefficient <80 indicates an antagonistic effect.

[0033] Table 1 The results of toxicity test of the mixture of fluoxathiapiprolin and flonicamid against the yellow striped flea beetle

[0034]

[0035] 2. Determination of the combined effect of fluoxetine amide and flonicamid on whiteflies

[0036] The test method adopts the spray method. First, the original drug organic solvent is prepared into 5 series of concentration solutions for standby use, and the spray pressure of the potter spray tower is adjusted to 1.47×10 5 Pa, the spray head was first cleaned twice with acetone, and then cleaned twice with distilled water for later use. Use a brush to select 10 whiteflies of the same physiological state and put them into a culture dish, and then place the culture dish on the bottom plate of the potter spray tower for spraying. The spray volume is 1 ml. After the liquid settles for 1 minute, the test insects are taken out and cultured in a constant temperature incubator at a temperature of 26±1℃, a humidity of 70%, and a light cycle of L:D=(16:8)h for breeding. Each treatment was repeated 4 times, and the clean water treatment was set as a blank control. After 48 hours, the number of dead insects was checked, and the total number of insects and the number of dead insects were recorded. The insect body was moved with a brush, and the test insect was considered dead if it was completely motionless.

[0037] The mortality calculation method and the combined effect evaluation method are the same as those in Specific Example 1.

[0038] Table 2 The results of toxicity test of fluoxetine amide and fluphenazine amide mixed to whitefly

[0039]

[0040] 3. Determination of the combined effect of fluoxetine and flonicamid on thrips

[0041] The test method adopts the leaf tube drug film method. First, fill the centrifuge tube with 1.5ml of the drug solution to be tested, one concentration per tube, and repeat each concentration 4 times. Then, place the centrifuge tube filled with the drug solution for 4 hours, pour out the drug solution, dry it, and then use scissors to cut a small hole with a diameter of about 3mm at the bottom of the centrifuge tube. Set the clean water treatment as the blank control.

[0042] Take fresh and clean cabbage leaves, use a hole puncher to make leaf discs with a diameter of 1 cm, then soak the cabbage leaf discs in each concentration of drug solution for 10 seconds, then place them at room temperature to dry naturally, and finally use tweezers to put the leaf discs and filter paper pieces of the same size into centrifuge tubes with the corresponding drug concentration, one piece per tube, to form leaf tube drug films.

[0043] Finally, 20 healthy thrips test insects of uniform size were inoculated and placed in a constant temperature box for breeding at a temperature of 26±1°C, a humidity of 65% to 70%, and a light cycle of L:D=(16:8)h. The death of the test insects was checked after 48h.

[0044] The mortality calculation method and the combined effect evaluation method are the same as those in Specific Example 1.

[0045] Table 3 The results of toxicity test of fluoxetine amide and fluphenazine amide mixed to thrips

[0046]

[0047] It can be seen from Tables 1, 2 and 3 that the combination of fluoxetine and flubendiamide in the range of 20:1-1:50 shows a certain synergistic effect on yellow flea beetles, whiteflies and thrips, especially in the range of 1:2-1:4, the synergistic effect is most significant.

[0048] The present invention will be further described below in conjunction with the formulation examples.

[0049] Preparation Example 1

[0050] Fluoxathiapiprolin 10%, flonicamid 40%, sodium lignin sulfonate 6%, sodium dibutylnaphthalene sulfonate 5%, GY-D06 6%, sodium dodecyl sulfate 5%, light calcium added to 100% by weight. The above raw materials are subjected to the conventional method for preparing water-dispersible granules, i.e., mixing, ultrafine airflow pulverization, mixing, and granulation steps to prepare 50% fluoxathiapiprolin·fluonicamid water-dispersible granules.

[0051] Preparation Example 2

[0052] Fluoxazolidinone 10%, flonicamid 20%, alkylphenol formaldehyde resin polyoxyethylene ether 5%, alkylnaphthalene formaldehyde condensate sodium sulfonate 5%, alkyl sodium sulfate 3%, potassium sorbate 3%, polyethylene glycol 2%, bentonite 2%, octanol 2%, deionized water is added to 100% by weight. The above raw materials are mixed, dispersed at high speed shear for 30 minutes, and sanded to a particle size of D using a sand mill. 90 When the particles are smaller than 10 μm, a 30% fluoxathiapiprole·flubenzuronide suspension is prepared.

[0053] Preparation Example 3

[0054] Weigh 5% fluoxazolidinone, 10% flonicamid, 6% lakai powder, 5% polyoxyethylene polyoxypropylene ether block copolymer, 5% sodium alkyl sulfate, 2% carboxymethyl cellulose, 1% potassium sorbate, 1% isothiazolinone, 2% butanol, and add deionized water to 100% by weight. The above raw materials are mixed, dispersed at high speed shear for 30 minutes, and sanded to a particle size D using a sand mill. 90 When the particles are smaller than 10 μm, a 15% fluoxathiapiprole·flubenzuronide suspension is prepared.

[0055] Biological Example 1: Field efficacy test for controlling greenhouse whitefly.

[0056] In 2022, the inventor conducted field efficacy tests on Formulation Example 1, Formulation Example 2, Formulation Example 3 and a control agent for controlling greenhouse whiteflies. The test crop was tomato, and the control effect of the agent on whiteflies and its safety on tomatoes were verified.

[0057] Experimental method: The plots were randomly arranged in groups, with each plot measuring 20 m 2 Each treatment was repeated 4 times, with a total of 6 treatments and 24 plots. Isolation devices were set between each plot, and the control was treated with clean water. When the occurrence of whitefly adults was large, a backpack electric sprayer was used for spraying.

[0058] Survey method: Survey the insect population base before spraying, and survey once at 1, 3, and 7 days after spraying, for a total of 4 surveys. Sampling was conducted at 5 points in each plot, with 2 plants at each point, for a total of 10 seedlings. Each plant had 2 leaves in the upper, middle, and lower layers, and the number of adults on each leaf was surveyed and calculated to calculate the insect population reduction rate and control effect.

[0059] Calculation method: Insect population reduction rate (%) = (insect population base before medication - insect population base after medication) / insect population base before medication × 100

[0060] Control effect (%) = (insecticidal reduction rate in treated area - insecticidal reduction rate in control area) / (1 - insecticidal reduction rate in control area) × 100

[0061] Table 4 Results of field efficacy test on whitefly

[0062]

[0063] The effects of each formulation example and the control agent on the prevention and control of cucumber aphids are shown in Table 4. One day after the application, the control effect of each treatment reached more than 88%, and the control agent control effects were 81.24% and 83.34% respectively; three days after the application, the control effect of each treatment reached more than 89%, and the control agent control effects were 82.35% and 84.38% respectively; seven days after the application, the control effect of each treatment reached more than 92%, with the highest being 95.57%, and the control agent control effects were 85.22% and 86.35% respectively. It can be seen that the control effect of each treatment 1 day, 3 days, and 7 days after the application was higher than that of the control agent, indicating that the composition preparation of the present invention has good quick-acting effect and long lasting effect during field application.

[0064] The results of the safety investigation during the trial showed that the treatments and control agents did not cause phytotoxicity to tomatoes, and no effects on other organisms such as non-target organisms or beneficial organisms were observed.

Claims

1. An insecticidal composition containing fluorine-containing oxazole amide, characterized in that: The insecticide composition comprises two active ingredients, wherein the first active ingredient is flubendiamide, and the second active ingredient is flubendiamide, and the weight ratio of the first active ingredient to the second active ingredient is 20:1-1:

40.

2. The insecticidal composition containing fluorine-containing oxazole amide according to claim 1, characterized in that: The mass percentage of the sum of the weight of the first active ingredient and the second active ingredient in the insecticide composition is 1% to 90% of the total weight of the insecticide composition, and the remainder is agriculturally acceptable carriers and adjuvants.

3. The insecticidal composition containing fluorine-containing oxazole amide according to claim 1, characterized in that: The preparation form of the insecticide composition is suspension, water dispersible granule, dispersible oil suspension, wettable powder, aqueous emulsion, microemulsion and suspoemulsion.

4. Use of the insecticidal composition containing fluorine oxazole amide according to claim 1 in controlling agricultural pests.

5. The use according to claim 4, characterized in that: The agricultural pests are yellow striped flea beetle, whitefly and thrips.

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