Biological agent for preventing and treating mango powdery mildew, preparation method and application thereof

Abstract

The application discloses a biological agent for preventing and treating mango powdery mildew, a preparation method and use method thereof, and the preparation method of the biological agent for preventing and treating mango powdery mildew comprises the following steps: inoculating activated Paenibacillus chibensis seed liquid into a plant alcohol extract, and carrying out fermentation culture under the condition that the temperature is 30-34 DEG C and the rotating speed is 150-180 r / min until more than 95% of bacteria in the fermentation liquor produce spores, and the number of the spores is greater than 1*10 9 CFU / mL. The biological agent for preventing and treating mango powdery mildew is prepared by fermenting Paenibacillus chibensis in a plant alcohol extract, the preparation method is simple, raw materials are green and environment-friendly, the production cost is low, the mango powdery mildew can be prevented and treated efficiently, and the planting environment is not polluted.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological control. More particularly, the present application relates to a biological preparation for preventing and treating mango powdery mildew, and a preparation method and application thereof. BACKGROUND

[0002] Mango has a sweet smell and is rich in sugar, vitamins and proteins. The fructose and glucose components in mango can be quickly absorbed by the small intestine of the human body, helping the human body recover physical strength and reduce the risk of low blood sugar. Mango is a high-fiber food that contains dietary fiber that can promote bowel movements and prevent constipation. In addition, vitamin A in mango can maintain the integrity of the skin and mucous membrane, and vitamin C can inhibit melanin synthesis, thereby having the effect of whitening and nourishing the skin. It can be seen that mango is a fruit with very high nutritional value.

[0003] Mango is a common fruit in tropical and south subtropical regions, and it is not resistant to low temperature and grows in areas with an average annual temperature of more than 22℃. China is the second largest mango producing country in the world, and Guangxi is the main mango producing area in China. The mango planting area in Guangxi reaches 1.54 million mu, the yield reaches 800,000 tons, and the fresh fruit output value reaches 5.1 billion yuan, accounting for about 30% of the country. There are nearly 400,000 households and 1.6 million people engaged in mango and related industries in Guangxi, and the yield ranks first in the country. Mango is an important economic crop in Guangxi.

[0004] Mango powdery mildew is a disease caused by Oidium mangiferae and occurs on mango. It mainly harms inflorescences, young leaves and young fruits, and the diseased part turns brown with white powdery small spots scattered on it. After expansion, they merge into a pale mycelium layer. After the inflorescences are infected, the flowers do not open and the diseased flowers fall off. Some flowers pollinate and set fruit, but the fruit falls off when it grows to the size of a bean. The diseased branches and flower stalks turn black, and the young leaves develop a white powdery mycelium layer on the back of the leaves.

[0005] Temperature conditions are the main factors affecting the prevalence of mango powdery mildew. When the monthly average temperature is 21-22℃, it is most suitable for the occurrence of mango powdery mildew, and a relative humidity of about 70% is more conducive to the occurrence of mango powdery mildew. During the "back to the south" period from March to April every year in Guangxi, both the temperature and the air humidity meet the best conditions for the prevalence of mango powdery mildew. This is also an important period for the formation of flowers and fruits of mango in Guangxi. Once mango is infected with this disease, it will cause great losses. Mango powdery mildew is also one of the main diseases of mango in Guangxi.

[0006] At present, the common treatment for mango powdery mildew is to use chemical agents such as methylthiophanate, fenpropimorph, and nitromite to prevent and control it. However, these chemical agents have high toxicity and can kill various organisms indiscriminately, including some beneficial animals such as frogs, bees and earthworms. The decrease of beneficial animals will lead to an increase in the number of pests, thereby affecting agricultural production.

[0007] In recent years, the national agricultural department also found that the harm caused by high dependence on pesticides and fertilizers, and attaches great importance to the research and popularization of the technology of reducing the use of pesticides and fertilizers. Therefore, for the important economic crops of mango in Guangxi, it is of great significance to develop a kind of green, non-toxic and efficient biological agent for preventing and treating mango powdery mildew, which is of great significance to the green planting of mango. SUMMARY

[0008] An object of the present application is to solve at least the above problems and provide at least the advantages to be described later.

[0009] Another object of the present application is to provide a biological agent for preventing and treating mango powdery mildew and its preparation method and use method, wherein the biological agent for preventing and treating mango powdery mildew is prepared by fermentation of the Bacillus cereus in the plant alcohol extract, the preparation method is simple, the raw materials are green and environmentally friendly, has the advantages of low production cost, high efficiency in preventing and treating mango powdery mildew, and no pollution to the planting environment.

[0010] In order to achieve these objects and other advantages according to the present application, a preparation method of a biological agent for preventing and treating mango powdery mildew is provided, comprising the following steps: inoculating activated Bacillus cereus seed liquid into plant alcohol extract, and carrying out fermentation culture under the condition that the temperature is 30-34℃ and the rotation speed is 150-180r / min, until more than 95% of the bacteria in the fermentation broth produce spores, and the number of spores is greater than 1×10 9 CFU / mL.

[0011] Preferably, the plant alcohol extract is a sugarcane residue alcohol extract.

[0012] Preferably, the preparation method of the sugarcane residue alcohol extract comprises the following steps:

[0013] Step 1, high-pressure steam treatment of sugarcane residue;

[0014] Step 2, adding 1-2 times the volume of boiling water to the sugarcane residue after high-pressure steam treatment, stirring into paste, and quickly freezing, taking out after freezing, and thawing at room temperature;

[0015] Step 3, mixing the freeze-thawed paste with ethanol solution, immersing at room temperature, filtering, repeating the operation of immersion, filtration and filtration three times, combining the three filtrates, and then performing vacuum distillation to remove ethanol to obtain the sugarcane residue alcohol extract.

[0016] Preferably, the Bacillus cereus seed liquid is inoculated into the plant alcohol extract at an inoculation amount of 5%.

[0017] Preferably, the Paenibacillus kribbensis is Paenibacillus kribbensis TRCC82001, with the preservation number of CGMCC No.7996.

[0018] Preferably, in step 1, before high-pressure steam treatment, the bagasse is cleaned and dried, and crushed into a powder with a particle size of 50-100 mesh to obtain a bagasse powder.

[0019] Preferably, in step 1, the high-pressure steam treatment is specifically: spraying the bagasse powder with high-pressure steam with a temperature of 220.75 DEG C and a pressure of 2.25 Mpa; while tumbling the bagasse powder to make it fully contact with the high-pressure steam; and the treatment time is 5 min.

[0020] Preferably, in step 2, the freezing temperature is -25 DEG C, and the freezing time is 24 h; in step 3, the mass fraction of the ethanol solution is 95%, the mass ratio of the freeze-thawed paste to the ethanol solution is 1:3, and the extraction time is 3 h.

[0021] The application also provides a biological agent for preventing and treating mango powdery mildew, which is prepared according to the preparation method of the biological agent for preventing and treating mango powdery mildew according to any one of claims 1-8.

[0022] The application also provides a use method of the biological agent for preventing and treating mango powdery mildew, which is diluted by 200-400 times and sprayed on the leaves of mango trees.

[0023] The application at least has the following beneficial effects:

[0024] The application uses Paenibacillus kribbensis to ferment in a plant alcohol extract to prepare a biological agent for preventing and treating mango powdery mildew, which has the advantages of simple preparation method, green and environmentally-friendly raw materials, low production cost, high efficiency in preventing and treating mango powdery mildew, and no pollution to the planting environment.

[0025] Other advantages, objects, and features of the application will be in part apparent and in part pointed out below. DETAILED DESCRIPTION

[0026] The application will be further described in detail below with reference to the examples, so that those skilled in the art can implement the application according to the description.

[0027] It should be noted that, in the following implementation, the experimental methods are conventional methods unless otherwise specified, and the reagents and materials can be obtained from commercial channels unless otherwise specified.

[0028] The application provides a preparation method of a biological agent for preventing and treating mango powdery mildew, and comprises the following steps: inoculating activated Paenibacillus kribbensis seed liquid into a plant alcohol extract, and performing fermentation culture under the condition that the temperature is 30-34 DEG C and the rotating speed is 150-180 r / min until more than 95% of the bacteria in the fermentation liquor produce spores, and the number of the spores is greater than 1*10 9 CFU / mL, sealing the tank, and obtaining the biological agent; wherein the air input amount during the fermentation culture is 1 / 20 of the volume of the fermentation tank.

[0029] In the technical scheme, the biological agent for preventing and treating mango powdery mildew is prepared by fermenting Paenibacillus kribbensis in a plant alcohol extract, and has the advantages of simple preparation method, green and environment-friendly raw material, low production cost, high efficiency in preventing and treating mango powdery mildew, and no pollution to the planting environment.

[0030] In another technical scheme, the plant alcohol extract is a sugarcane residue alcohol extract; the use of the sugarcane residue alcohol extract as the raw material can reduce the production cost and improve the resource utilization rate while ensuring the prevention and treatment effect of the biological agent.

[0031] In another technical scheme, the preparation method of the sugarcane residue alcohol extract comprises the following steps:

[0032] Step 1, performing high-pressure steam treatment on the sugarcane residue;

[0033] Step 2, adding 1-2 times the volume of boiling water into the sugarcane residue after the high-pressure steam treatment, stirring into a paste, and obtaining the paste; and quickly freezing, taking out after freezing, and thawing at room temperature;

[0034] Step 3, mixing the paste after freezing and thawing with an ethanol solution, performing extraction at room temperature, and repeatedly performing extraction, filtration and extraction, filtration and extraction three times; combining the three filtrates, and performing vacuum distillation to remove the ethanol, and obtaining the sugarcane residue alcohol extract.

[0035] Before the alcohol extraction, the sugarcane residue is subjected to high-pressure steam treatment and freezing and thawing to break the wall of the sugarcane residue, instead of using a special wall-breaking device, so that the method is simple, the wall-breaking effect is ensured, the equipment investment is reduced, the production cost is further reduced, and the energy consumption is reduced.

[0036] In another technical scheme, the Paenibacillus kribbensis seed liquid is inoculated into the plant alcohol extract at an inoculation amount of 5%.

[0037] In another technical scheme, the Paenibacillus kribbensis is Paenibacillus kribbensis (TRCC82001), and the preservation number is CGMCC No.7996.

[0038] In another technical solution, in step 1, before high-pressure steam treatment, the bagasse is cleaned and dried, and is crushed into a powder with a mesh size of 50-100, to obtain a bagasse powder; the quality of the bagasse extract is improved, and the effect of high-pressure steam treatment and freeze-thawing of the bagasse is ensured.

[0039] In another technical solution, in step 1, the high-pressure steam treatment specifically comprises: spraying the bagasse powder with high-pressure steam with a temperature of 220.75 DEG C and a pressure of 2.25 Mpa; and tumbling the bagasse powder to ensure that it is in full contact with the high-pressure steam; and the treatment time is 5 min.

[0040] In another technical solution, in step 2, the freezing temperature is -25 DEG C, and the freezing time is 24 h; in step 3, the mass fraction of the ethanol solution is 95%, the mass ratio of the freeze-thawed paste to the ethanol solution is 1:3, and the extraction time is 3 h.

[0041] The application further provides a biological agent for preventing and treating mango powdery mildew, which is prepared according to the preparation method of the biological agent for preventing and treating mango powdery mildew.

[0042] The application further provides a use method of the biological agent for preventing and treating mango powdery mildew.

[0043] In a non-epidemic season of mango powdery mildew, the biological agent and water are mixed uniformly at a volume ratio of 1:400, and after dilution, the mango tree leaf surface is sprayed, and prevention is performed in advance; the use amount of the mixed solution per mu is 200-300 kg, and spraying is performed once a month;

[0044] In an epidemic season of mango powdery mildew, when mango powdery mildew does not occur, the biological agent and water are mixed uniformly at a volume ratio of 1:400, and after dilution, the mango tree leaf surface is sprayed; the use amount of the mixed solution per mu is 200-300 kg, and spraying is performed twice a month;

[0045] When mango powdery mildew occurs, the biological agent and water are mixed uniformly at a volume ratio of 1:200, and after dilution, the mango tree leaf surface is sprayed; the use amount of the mixed solution per mu is 200-300 kg, and spraying is performed once every 3 days, and spraying is continuously performed for 3-5 times; after the powdery mildew is improved, the biological agent and water are mixed uniformly at a volume ratio of 1:300, and after dilution, the mango tree leaf surface is sprayed; the use amount of the mixed solution per mu is 200-300 kg, and spraying is performed twice a month.

[0046] <Embodiment 1>

[0047] A preparation method of a biological agent for preventing and treating mango powdery mildew comprises the following steps:

[0048] Step one, clean and dry the bagasse, and crush it into 50-100 mesh powder, get the bagasse powder;

[0049] Step two, use the steam generator to spray high pressure steam on the bagasse powder, the temperature is 220.75℃, the pressure is 2.25Mpa; at the same time, use the machine to turn and toss the bagasse powder, so that it fully contacts with the high pressure steam; the treatment time is 5min;

[0050] Step three, add equal volume of boiling water to the bagasse powder treated by high pressure steam, stir into paste, get the paste; and quickly freeze it under the condition of-25℃ for 24h, take it out after freezing, thaw it at room temperature;

[0051] Step four, mix the freeze-thawed paste with 95% ethanol solution according to the mass ratio of 1:3, immerse it at room temperature for 3h, filter, repeat the operation of immersion, filtration three times, combine the three filtrates, then distill under reduced pressure to remove ethanol, get the bagasse alcohol extract;

[0052] Step five, add 3 times volume of water to the bagasse alcohol extract, stir evenly; then inoculate the activated Paenibacillus kribbensis seed liquid into the diluted bagasse alcohol extract with 5% inoculation amount, pour the inoculated bagasse alcohol extract into the fermentation tank, under the condition of temperature 30℃, rotation speed 150r / min, introduce air to carry out fermentation culture, the air introduction amount is 1 / 20 of the volume of the fermentation tank, until more than 95% of the bacteria in the fermentation broth produce spores, and the number of spores is greater than 1×10 9 CFU / mL, seal the tank, get the biological preparation; wherein, the Paenibacillus kribbensis is provided by South China Agricultural University, the name is Paenibacillus kribbensis TRCC82001, the preservation number is CGMCC No.7996;

[0053] A use method of a biological preparation for preventing and treating mango powdery mildew, specifically:

[0054] In the season when mango powdery mildew does not prevail, mix the biological preparation and water uniformly according to the volume ratio of 1:400, dilute and then spray the mixture on the mango tree leaves, prevent in advance, the use amount of the mixture per mu is 200kg, spray once a month;

[0055] In the season when mango powdery mildew prevails, when mango powdery mildew does not occur, mix the biological preparation and water uniformly according to the volume ratio of 1:400, dilute and then spray the mixture on the mango tree leaves, the use amount of the mixture per mu is 200kg, spray twice a month;

[0056] When mango powdery mildew occurs, the biological agent and water are mixed uniformly at a volume ratio of 1:200, and after dilution, the mango tree leaf surface is sprayed, the use amount of the mixed solution per mu is 200 kg, and spraying is performed every 3 days for 3 times; after the powdery mildew is improved, the biological agent and water are mixed uniformly at a volume ratio of 1:300, and after dilution, the mango tree leaf surface is sprayed, the use amount of the mixed solution per mu is 200 kg, and spraying is performed twice a month.

[0057] <Embodiment 2>

[0058] A preparation method of a biological agent for preventing and treating mango powdery mildew, comprising the following steps:

[0059] Step one, the bagasse is cleaned and dried, and is crushed into a powder of 50-100 mesh to obtain a bagasse powder;

[0060] Step two, the bagasse powder is sprayed with high-pressure steam with a temperature of 220.75℃ and a pressure of 2.25Mpa by using a steam generator; at the same time, the bagasse powder is turned over by using a machine to make it fully contact with the high-pressure steam; the treatment time is 5min;

[0061] Step three, 2 times of boiling water are added to the bagasse powder treated by the high-pressure steam, and the mixture is stirred into a paste to obtain a paste; the paste is quickly frozen at a temperature of-25℃ for 24h, and after being taken out, the paste is thawed at room temperature;

[0062] Step four, the freeze-thawed paste is mixed with an ethanol solution with a mass fraction of 95% at a mass ratio of 1:3, and is immersed at room temperature for 3h; the immersion is repeated three times, and the filtrates are combined; the combined filtrates are subjected to reduced pressure distillation to remove the ethanol to obtain a bagasse alcohol extract;

[0063] Step five, 3 times of water are added to the bagasse alcohol extract and stirred uniformly; activated Paenibacillus kribbensis seed liquid is inoculated into the diluted bagasse alcohol extract at an inoculation amount of 5%, and the inoculated bagasse alcohol extract is poured into a fermentation tank; air is introduced into the fermentation tank at a temperature of 34℃ and a rotation speed of 180r / min, and the air introduction amount is 1 / 20 of the volume of the fermentation tank; until more than 95% of the bacteria in the fermentation broth produce spores, and the number of the spores is greater than 1×10 9 CFU / mL, the fermentation tank is sealed to obtain the biological agent; wherein, the Paenibacillus kribbensis is provided by South China Agricultural University, and the name is Paenibacillus kribbensis TRCC82001, and the preservation number is CGMCC No.7996;

[0064] A use method of a biological agent for preventing and treating mango powdery mildew, specifically:

[0065] In the non-epidemic season of mango powdery mildew, the biological agent and water are mixed uniformly at a volume ratio of 1:400, and after dilution, the mango tree leaf surface is sprayed, and the prevention is advanced, the use amount of mixed solution per mu is 300 kg, and it is sprayed once a month;

[0066] In the epidemic season of mango powdery mildew, when the mango powdery mildew does not occur, the biological agent and water are mixed uniformly at a volume ratio of 1:400, and after dilution, the mango tree leaf surface is sprayed, the use amount of mixed solution per mu is 300 kg, and it is sprayed twice a month;

[0067] When the mango powdery mildew occurs, the biological agent and water are mixed uniformly at a volume ratio of 1:200, and after dilution, the mango tree leaf surface is sprayed, the use amount of mixed solution per mu is 300 kg, and it is sprayed once every 3 days for 5 times; after the powdery mildew is improved, the biological agent and water are mixed uniformly at a volume ratio of 1:300, and after dilution, the mango tree leaf surface is sprayed, the use amount of mixed solution per mu is 300 kg, and it is sprayed twice a month.

[0068] <Comparative Example 1>

[0069] A preparation method of a biological agent for preventing and treating mango powdery mildew, which is different from example 2 in that steps one to three are not passed through, and then in step four, the bagasse is directly mixed with the ethanol solution for alcohol extraction; other conditions and parameters are the same as example 2.

[0070] <Comparative Example 2>

[0071] A preparation method of a biological agent for preventing and treating mango powdery mildew, which is different from example 2 in that steps two to three are not passed through, and then in step four, the bagasse powder is mixed with the ethanol solution for alcohol extraction; other conditions and parameters are the same as example 2.

[0072] <Comparative Example 3>

[0073] A preparation method of a biological agent for preventing and treating mango powdery mildew, which is different from example 2 in that step two is not passed through, and then in step four, the frozen and thawed paste is mixed with the ethanol solution for alcohol extraction; other conditions and parameters are the same as example 2.

[0074] <Comparative Example 4>

[0075] A preparation method of a biological agent for preventing and treating mango powdery mildew, which is different from example 2 in that step three is not passed through, and then in step four, the bagasse powder treated by high-pressure steam is mixed with the ethanol solution for alcohol extraction; other conditions and parameters are the same as example 2.

[0076] <Comparative Example 5>

[0077] A preparation method of a biological agent for preventing and treating mango powdery mildew, different from example 2 in that the bagasse is not subjected to steps 1-3, but is subjected to wall breaking by a special wall breaking device after being washed and dried, and then in step 4, the bagasse subjected to the special wall breaking device is mixed with an ethanol solution for alcohol extraction; other conditions and parameters are the same as in example 2.

[0078] The fermentation time of examples 1-2 and comparative examples 1-5 is recorded, the fermentation time being the duration from the start of fermentation to the fermentation liquid reaching the required duration, and from the fermentation time of 20h, the fermentation liquid is detected once every 2h to determine whether it meets the requirements, thereby determining the fermentation time of each example and each comparative example; the fermentation time of examples 1-2 is 24h, and the fermentation time of comparative examples 1-5 is 60h, 54h, 40h, 44h and 26h, respectively;

[0079] Compared with comparative examples 1-4, the fermentation time of example 2 is significantly shortened, and the bagasse of the present application is subjected to crushing, high-pressure steam treatment and freeze-thawing in sequence before alcohol extraction, achieving good wall breaking of the bagasse and significantly shortening the fermentation time;

[0080] Compared with comparative example 5, the fermentation time of example 2 is slightly shortened, and the bagasse of the present application is subjected to crushing, high-pressure steam treatment and freeze-thawing in sequence before alcohol extraction, which does not have a worse wall breaking effect than using a special wall breaking device, and the method is simple, which can reduce equipment investment, reduce production cost and reduce energy consumption while ensuring the wall breaking effect.

[0081] <Field efficacy test>

[0082] Test agent: biological agent prepared in examples 1-2;

[0083] Control agent: 20% triadimefon emulsion (Difenoconazole, Sichuan Guoguang Agricultural Chemical Co., Ltd.);

[0084] Test site: three mango test sites were selected, test site 1: Baise Mango Plantation in Tianyang County, Baise City, Guangxi (Baise); test site 2: Guiqi Mango Base in Long'an, Guangxi (Long'an); test site 3: Baila Mango Industry Base in Linfen Village, Beijiang Township, Ningming County, Chongzuo City, Guangxi (Chongzuo);

[0085] Test method:

[0086] Test point 1 (Baise): In late February 2022, three plots of 2 mu of mango land were selected as test plots in Baise Mango Orchard in Tianyang District, Baise City, Guangxi. The mango varieties in each test plot were the same, the planting dates were the same, and the growth conditions and fertilizer and water management were consistent. Test group 1, test group 2 and control group used the biological agents prepared in Examples 1-2 and the control agent to prevent and control mango powdery mildew in the test plots, respectively. Conventional management was used in the field.

[0087] Test point 2 (Long'an): In early March 2022, three plots of 2.5 mu of mango land were selected as test plots in Guangxi Long'an Guiqi Mango Base. The mango varieties in each test plot were the same, the planting dates were the same, and the growth conditions and fertilizer and water management were consistent. Test group 1, test group 2 and control group used the biological agents prepared in Examples 1-2 and the control agent to prevent and control mango powdery mildew in the test plots, respectively. Conventional management was used in the field.

[0088] Test point 3 (Chongzuo): In early March 2022, three plots of 2 mu of mango land were selected as test plots in Bailing Mango Industry Base in Linfen Village, Beijiang Township, Ningming County, Chongzuo City, Guangxi. The mango varieties in each test plot were the same, the planting dates were the same, and the growth conditions and fertilizer and water management were consistent. Test group 1, test group 2 and control group used the biological agents prepared in Examples 1-2 and the control agent to prevent and control mango powdery mildew in the test plots, respectively. Conventional management was used in the field.

[0089] The use method of the biological agents prepared in Examples 1-2 was carried out according to the use method of Examples 1-2; the use method of the control agent was as follows: in the non-epidemic season of mango powdery mildew, 20% triadimefon emulsion (Fungin) was diluted 1500 times, and the mango tree foliage was sprayed, the dosage of diluent per mu was 300 kg, and spraying was carried out once a month; in the epidemic season of mango powdery mildew, when mango powdery mildew did not occur, 20% triadimefon emulsion (Fungin) was diluted 1500 times, and the mango tree foliage was sprayed, the dosage of diluent per mu was 300 kg, and spraying was carried out twice a month; when mango powdery mildew occurred, 20% triadimefon emulsion (Fungin) was diluted 800 times, and the mango tree foliage was sprayed, the dosage of diluent per mu was 300 kg, and spraying was carried out once every 3 days for 5 times; after the powdery mildew improved, 20% triadimefon emulsion (Fungin) was diluted 1000 times, and the mango tree foliage was sprayed, the dosage of diluent per mu was 300 kg, and spraying was carried out twice a month;

[0090] During the epidemic (back to the south) season of powdery mildew at the three test points, test group 1 and test group 2 of each test point did not have mango trees with powdery mildew, while the control group had a large area of powdery mildew; it is shown that the biological agents prepared in Examples 1-2 have good prevention and control effect on mango powdery mildew.

[0091] In the mango harvest season, each test point respectively statistics test group 1, test group 2 and control group each mu for the prevention and treatment of mango powdery mildew cost (yuan / mu) and the average mu yield of mango (kg / mu), the statistical results are shown in table 1;

[0092] Table 1 statistical results

[0093]

[0094] As shown in table 1, in the three test points, compared with the control group, the prevention and treatment cost of test group 1 and test group 2 per mu for mango powdery mildew is significantly reduced, the biological agent prepared by examples 1-2 is used to prevent and treat mango powdery mildew, and in the mango powdery mildew epidemic season, the mango tree does not occur powdery mildew, the spraying frequency is reduced, the amount of biological agent and labor cost are reduced, and the prevention and treatment cost can be significantly reduced; Moreover, compared with the control group, the average mu yield of test group 1 and test group 2 is significantly improved, the biological agent prepared by examples 1-2 has good prevention and treatment effect on mango powdery mildew, the mango tree does not occur powdery mildew, and the yield of mango can be effectively improved;

[0095] The biological agent for preventing and treating mango powdery mildew is prepared by fermentation of the Klebsiella sp. in the alcohol extract of sugarcane residue, the preparation method is simple, the raw materials are green and environmentally friendly, the mango powdery mildew can be efficiently prevented and treated, the production cost is low, the planting environment is not polluted, the sugarcane residue is subjected to smashing, high-pressure steam treatment and freeze-thawing in sequence before alcohol extraction, the sugarcane residue is broken, the method is simple, the breaking effect is guaranteed, the equipment investment is reduced, the production cost is further reduced, and the energy consumption is reduced.

[0096] Although the embodiments of the present application have been disclosed as above, it is not limited to the application listed in the specification and embodiments, and can be fully applied to various fields suitable for the present application, and other modifications can be easily realized by those skilled in the art, therefore, the present application is not limited to specific details, but is limited to the general concept defined by the claims and equivalent scope.

Claims

1. A method for preparing a biological agent for controlling powdery mildew of mango, characterized in that, The method comprises the following steps: The activated Paenibacillus chibensis seed liquid is inoculated into the plant alcohol extract, and is subjected to fermentation culture under the condition that the temperature is 30-34 ℃ and the rotating speed is 150-180 r / min, until more than 95% of the bacteria in the fermentation liquid produce spores, and the number of the spores is greater than 1×10 9 CFU / mL. 9 CFU / mL. The plant alcohol extract is a bagasse alcohol extract; The preparation method of the bagasse alcohol extract comprises the following steps: Step 1, high-pressure steam treatment is performed on the bagasse; Step 2, 1-2 times volume of boiling water is added to the bagasse after high-pressure steam treatment, and the mixture is stirred into a paste, and the paste is quickly frozen, and then taken out and thawed at room temperature; Step 3, the frozen-thawed paste is mixed with an ethanol solution, and then immersed at room temperature, filtered, and the operation of immersion and filtration is repeated three times, and then the three filtrates are combined, and then subjected to reduced-pressure distillation to remove ethanol, thereby obtaining the bagasse alcohol extract; In step 2, the freezing temperature is-25 DEG C, and the freezing time is 24 hours; in step 3, the mass fraction of the ethanol solution is 95%, the mass ratio of the frozen-thawed paste to the ethanol solution is 1:3, and the immersion time is 3 hours.

2. The method of claim 1, wherein the biological agent is prepared by the steps of: The Paenibacillus kribbensis seed liquid is inoculated into the plant alcohol extract at an inoculation amount of 5%.

3. The method for preparing the biological agent for controlling mango powdery mildew as described in claim 1, characterized in that, The Paenibacillus chibensis is Paenibacillus chibensis (ATCC® 29743®) Paenibacillus kribbensis ) TRCC82001, with the preservation number of CGMCC No. 7996.

4. The method for preparing the biological agent for controlling mango powdery mildew as described in claim 1, characterized in that, In step 1, before high-pressure steam treatment, the bagasse is cleaned and dried, and then crushed into a powder with a particle size of 50-100 mesh, thereby obtaining a bagasse powder.

5. The method for preparing the biological agent for controlling mango powdery mildew as described in claim 4, characterized in that, In step 1, the high-pressure steam treatment is specifically as follows: the bagasse powder is sprayed with high-pressure steam with a temperature of 220.75 DEG C and a pressure of 2.25 Mpa, and the bagasse powder is simultaneously turned over to make it fully contact with the high-pressure steam, and the treatment time is 5 minutes.

6. A biological preparation for controlling powdery mildew of mango characterized in that, The biological agent for preventing and treating mango powdery mildew is prepared by the method according to any one of claims 1-5.

7. The method of using the biological agent for controlling powdery mildew of mango as claimed in claim 6, wherein, The biological agent is diluted by 200-400 times, and then sprayed on the leaves of mango trees.

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