A method for increasing the content of plant active selenium in selenium rape stems and its application

Abstract

The present invention provides a method for increasing the content of plant active selenium in selenium-rich rape shoots, belonging to the technical field of microbial fermentation. The main steps are as follows: Inoculate Leuconostoc mesenteroides subsp. mesenteroides or Lactobacillus plantarum into an MRS medium containing pectic acid and succinic acid and culture for 36 - 72 h. Then evenly sprinkle the bacterial liquid on the selenium-rich rape shoots after blanching treatment for aerobic fermentation, dry and crush after heat treatment. Finally, add the dried selenium-rich dehydrated rape shoots to water, a fermentation carbon source and yeast for fermentation for 3 - 6 h to obtain selenium-rich rape shoots rich in plant active selenium. The present invention uses two common microorganisms, lactic acid bacteria and yeast, and improves the content of plant active selenium in selenium-rich rape shoots through semi-solid fermentation and liquid fermentation methods.

Description

Technical Field

[0001] The present invention belongs to the field of microbial applications, and specifically relates to a method for increasing the content of plant active selenium in selenium-rich rape shoots and its application in fruit and vegetable juices. Background Art

[0002] Rape is one of the four traditional oil crops in China and also one of the four major oil crops in the world. In China, the rape industry can be developed from south to north and from east to west. Nowadays, in addition to being used for oil extraction, rape seedlings or rape shoots can be eaten, and rape flowers can also be used for viewing and have various important values. The "oil-vegetable dual-purpose" rape new variety - Zhongyouza 19 developed by the Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences. Rape shoots are bright green in color, crispy and tender in taste, rich in nutrients, containing trace elements such as carotene, vitamin C, selenium, calcium, etc., and have the effects of reducing blood lipid, detoxifying and detumescence, and strengthening the body.

[0003] Selenium is one of the essential trace elements for the human body. Lack of selenium will cause many negative effects such as decreased human immunity and malnutrition. Selenium in nature is generally divided into two types: plant active selenium and inorganic selenium. The main selenium that can be absorbed and utilized by the human body is plant active selenium, while inorganic selenium has greater toxicity and is not easily absorbed and utilized. Plant active selenium refers to that crops convert inorganic selenium into plant active selenium through photosynthesis and biological transformation in the crop body, and exists in the form of selenomethionine in the crop body. Plant active selenium has high biological activity, can meet the efficient absorption and metabolism of selenium by the human body, and the absorption rate by the human body is as high as over 99%. Plant active selenium combines with heavy metals and toxic organic compounds in the human body and excretes harmful substances through human metabolic channels such as sweat, respiration, excretion, etc., thus playing a protective role in human health. Summary of the Invention

[0004] The purpose of the present invention is to solve the problem that the content of plant active selenium in selenium-rich rape shoots is not high. Through microbial fermentation, part of the inorganic selenium in selenium-rich rape shoots is converted into plant active selenium, so as to increase the content of plant active selenium and promote the absorption and utilization of selenium in the human body.

[0005] A method for increasing the content of plant active selenium in selenium-rich rape shoots, comprising the following steps:

[0006] ① Heat blanch fresh selenium-rich rape shoots with an aqueous solution containing CaCl2;

[0007] ② Inoculate Leuconostoc mesenteroides subsp. mesenteroides or Lactobacillus plantarum into an MRS medium containing pectic acid and succinic acid and culture for 36 - 72 h, then evenly sprinkle the bacterial liquid on the selenium-rich rape shoots after heat blanching treatment, and perform aerobic fermentation at 37 ± 2 °C for 4 - 24 h to obtain fermented selenium-rich rape shoots;

[0008] ③ After heat treatment at 70-80 °C, adjust the temperature to 40-50 °C and dry until the moisture content is less than 8%.

[0009] ④ Add the dried selenium-rich dehydrated rape stems to water, fermentation carbon source and yeast, and ferment at 25-30 °C for 3-6 h. After fermentation, perform sterilization treatment to obtain rape stems rich in plant active selenium.

[0010] Among them, the preservation number of Leuconostoc mesenteroides subsp. mesenteroides is: CGMCC 1.3919, and the preservation number of Lactobacillus plantarum is: CGMCC 1.20.

[0011] Among them, the concentration of the aqueous solution of CaCl2 is 0.01-0.1%.

[0012] Among them, the mass-volume fraction of pectic acid and succinic acid is 0.1-1% of the final concentration of the whole culture medium.

[0013] Among them, heat treatment is performed at 70-80 °C for 1-3 min.

[0014] Among them, the fermentation carbon source includes sucrose and glucose.

[0015] Among them, the mass-volume ratio of the dried selenium-rich dehydrated rape stems to water is 1:1-1:4.

[0016] Among them, the addition amount of yeast is 0.2-2%, and the addition amount of fermentation carbon source is 0.3-4%.

[0017] . Among them, the yeast includes Angel yeast and Saccharomyces cerevisiae.

[0018] Beneficial effects

[0019] According to previous experimental studies, yeast has a certain selenium-accumulating ability. However, generally, yeast cannot obtain enough inorganic selenium from nature to become selenium-rich yeast. In the present invention, under the condition of using an acidic culture medium, after fermentation by Leuconostoc mesenteroides subsp. mesenteroides and Lactobacillus plantarum, the inorganic selenium in selenium-rich rape stems is converted into selenium that can be utilized by yeast. The specific components formed by the fermentation of these two lactic acid bacteria still need to be further verified by experiments. However, through the detection results, it can be found that after fermenting selenium-rich rape stems with Leuconostoc mesenteroides subsp. mesenteroides and Lactobacillus plantarum and then fermenting with Angel yeast and Saccharomyces cerevisiae, the content of plant active selenium has increased several times. Thus, it can be seen that the fermentation by lactic acid bacteria improves the utilization of selenium in selenium-rich rape stems by yeast, thereby increasing the content of plant active selenium in selenium-rich rape stems.

[0020] According to the detection results, the plant active selenium in the selenium-enriched rape bolts of the present invention is mainly selenomethionine. After fermentation by Leuconostoc mesenteroides subsp. mesenteroides and Lactobacillus plantarum and then treated with yeast, the content of plant active selenium has been significantly improved. However, Pediococcus pentosaceus and Streptococcus thermophilus, which are also lactic acid bacteria, have no effect. Generally speaking, Angel yeast has a better improvement effect than Saccharomyces cerevisiae. Without fermentation by Leuconostoc mesenteroides subsp. mesenteroides and Lactobacillus plantarum and then yeast fermentation, the content of plant active selenium has not been significantly improved. The best fermentation is the combination of Leuconostoc mesenteroides subsp. mesenteroides and Angel yeast. After fermentation, the plant active selenium in the dehydrated selenium-enriched rape bolts accounts for 84.84±0.37% of the total selenium content, showing a significant increase. Detailed implementation mode

[0021] In this example, Lactobacillus plantarum was isolated from the China General Microbiological Culture Collection Center, CGMCC 1.3919, and its Latin scientific name Lactobacillus plantarum , Leuconostoc mesenteroides subsp. mesenteroides was isolated from the China General Microbiological Culture Collection Center, CGMCC 1.20, and its Latin scientific name Leuconostoc mesenteroides subsp. Mensenteroides , Pediococcus pentosaceus was purchased from Shaanxi Senyuan Biotechnology Co., Ltd., and Streptococcus thermophilus was isolated from the China General Microbiological Culture Collection Center, CGMCC 1.1864, and its Latin scientific name Streptococcus thermophilus . Yeast 1 is the commercially available Angel yeast, and yeast 2 is Saccharomyces cerevisiae, which was isolated from the China General Microbiological Culture Collection Center, CGMCC 2.3871, and its Latin scientific name Saccharomyces cerevisiae .

[0022] The determination method of the content of plant active selenium (organic selenium) in the rape bolts of the present invention is based on the agricultural industry standard NY / T 3556-2020 of the People's Republic of China, and the pretreatment method has been improved.

[0023] The specific pretreatment method is as follows: Weigh 0.1 - 0.5 g (accurate to 0.001 g) of the sample to be tested, place it in a 15 mL centrifuge tube, add 5 mL of water and 20 mg of protease XIV, vortex for 30 s, perform ultrasonic bath at 37 °C for 3 h, and shake several times. Let it stand for 10 min, centrifuge at 9000 r / min at 4 °C for 10 min, take the supernatant and filter it through a 0.22 μm filter membrane for testing. At the same time, perform a blank test, store it in a 4 °C refrigerator before loading the machine, and complete the determination within 5 h. Use liquid chromatography inductively coupled plasma mass spectrometry to determine the selenium species, and the specific method can be seen in NY / T 3556-2020.

[0024] Example 1

[0025] This embodiment provides a method for increasing the content of plant active selenium in selenium-rich rape shoots. Among them, the selenium rape shoots are selected from the "oil-vegetable dual-purpose" Zhongyouza 19 rape shoots in the same plot in 2022, and the moisture content of the rape shoots is reduced to less than 8% under the condition of 40-50 °C to obtain selenium-rich dehydrated rape shoots. After testing, the total selenium content of the selenium-rich dehydrated rape shoots is 0.1629±0.016 mg / kg, and the content of plant active selenium is 0.0912±0.021 mg / kg, accounting for 55.99±0.17% of the total selenium content.

[0026] (1) Prepare a mixed blanching protective solution. In the blanching protective solution, the content of CaCl2 is 0.05%.

[0027] (2) Control the water temperature in the fruit and vegetable blanching machine between 80-90 °C. Then, select selenium-rich rape shoots with the same length, tender, free of pests and diseases, and mechanical damage, and put them into the blanching protective solution. Blanch them in the blanching pot of the fruit and vegetable blanching machine for 30-60 seconds to ensure that the selenium-rich rape shoots are evenly heated during the blanching process. After blanching, transfer the selenium-rich rape shoots to the cooling pot of the fruit and vegetable blanching machine, and use the bubble water cooling method. After cooling for 2-5 minutes, take out the selenium-rich rape shoots to obtain the blanched selenium-rich rape shoots.

[0028] (3) Inoculate Leuconostoc mesenteroides subsp. mesenteroides CGMCC 1.20 onto MRS medium, and incubate it at a constant temperature of 37 °C for 24-36 h for activation culture. After activation, dilute it with physiological saline to obtain the activated Leuconostoc mesenteroides subsp. mesenteroides.

[0029] (4) Preparation of selenium-protecting liquid MRS medium: Weigh 10 g of peptone, 10 g of beef extract, 5 g of yeast extract, 2 g of dipotassium hydrogen phosphate, 2 g of diammonium citrate, 5 g of sodium acetate, 20 g of glucose, 1 ml of Tween 80, 0.5 g of magnesium sulfate, 0.25 g of manganese sulfate, 2 g of pectic acid, and 3 g of succinic acid, and make up the volume to 1 L in a volumetric flask. Adjust the pH value to 6.2-6.4, and sterilize it under high pressure (101 Kpa, 121 °C) for 15 min to obtain the selenium-protecting liquid MRS medium.

[0030] (5) Inoculate the activated Leuconostoc mesenteroides subsp. mesenteroides into the selenium-protecting liquid MRS medium, and carry out static liquid culture for 36-72 h until the turbidity OD 600 of the culture solution is between 3.0 and 3.5 to obtain the fermentation broth of Leuconostoc mesenteroides subsp. mesenteroides.

[0031] (6) Evenly sprinkle the fermentation broth of Leuconostoc mesenteroides subsp. mesenteroides prepared in step (5) on the blanched selenium-rich rape shoots, and carry out aerobic fermentation at 37±2 °C for 24 h to obtain the fermented selenium-rich rape shoots.

[0032] Soak the selenium-rich rape bolts in clean water, and then place the rape bolts in an oven and treat them at 75 °C for 2 min to quickly kill the Leuconostoc mesenteroides subsp. mesenteroides remaining on the surface of the selenium-rich rape bolts. Subsequently, adjust the temperature to 45 °C and continue drying until the moisture content is less than 8%. Measure the plant active selenium to obtain selenium-rich dehydrated rape bolts.

[0033] (8)Crush the dried selenium-rich dehydrated rape bolts, mix them with water at a material-liquid ratio of 1:2 (m / V), add 1-4% sucrose according to the mass-volume ratio, homogenize and mix, then inoculate 1% Angel yeast by mass-volume ratio, and ferment at 30 °C for 3 h. After fermentation, sterilize and dry to a water content of 8%, measure the content of plant active selenium in it, and obtain selenium-rich rape bolts rich in plant active selenium.

[0034] Comparative experiment

[0035] The applicant also used Lactobacillus plantarum, Pediococcus pentosaceus, and Streptococcus thermophilus. The specific operation method was the same as that in Example 1, only replacing the fermenting microorganisms with an equal amount of Lactobacillus plantarum, Pediococcus pentosaceus, and Streptococcus thermophilus. During the fermentation process, take out a part of the selenium-rich rape bolts every 4 h, measure and dry them to 8%, and measure the total selenium and plant active selenium contents after dehydration. After the fermentation of the above four strains is completed, perform a drying treatment. Then divide the four portions of fermented rape bolts and one portion of unfermented rape bolts into two portions. One portion is inoculated with Angel yeast according to the method in Example 1, and the other portion is inoculated with Saccharomyces cerevisiae of CGMCC 2.3871, and perform aerobic fermentation at 30 °C for 3 h.

[0036] Content of plant active selenium in selenium-rich rape bolts with different fermentation times

[0037]

[0038] According to the test results, the plant active selenium of the present invention is mainly selenomethionine. It can be seen that after fermentation by Leuconostoc mesenteroides subsp. mesenteroides and Lactobacillus plantarum and then treated with Saccharomyces cerevisiae, the content of plant active selenium has been significantly improved. However, Pediococcus pentosaceus and Streptococcus thermophilus, which are also lactic acid bacteria, have no effect. Generally speaking, the improvement effect of Angel yeast is better than that of Saccharomyces cerevisiae. Without fermentation by Leuconostoc mesenteroides subsp. mesenteroides and Lactobacillus plantarum and then fermentation with yeast, the content of plant active selenium has not been significantly improved. The best fermentation is the combination of Leuconostoc mesenteroides subsp. mesenteroides and Angel yeast. After the fermentation is completed, the plant active selenium in the dehydrated selenium-rich rape bolts accounts for 84.84 ± 0.37% of the total selenium content, showing a significant improvement.

[0039] Example 2

[0040] This example provides a method for increasing the content of plant active selenium in selenium-rich rape bolts. The specific method is as follows.

[0041] (1)Prepare a mixed blanching protective solution. In the blanching protective solution, the content of CaCl2 is 0.1%.

[0042] (2)Control the water temperature in the fruit and vegetable blanching machine between 80 - 90 °C. Then put the selenium-rich rape bolts that are of the same length, tender, free from pests and diseases, and mechanical damage into the blanching protective solution and blanch them in the blanching pot of the fruit and vegetable blanching machine for 30 - 60 s, ensuring that the selenium-rich rape bolts are evenly heated during the blanching process. After blanching, transfer the selenium-rich rape bolts to the cooling pot of the fruit and vegetable blanching machine and cool them by the bubble water cooling method. After cooling for 2 - 5 min, take out the selenium-rich rape bolts to obtain the blanched selenium-rich rape bolts.

[0043] (3)Preparation of selenium-protecting liquid MRS medium: According to the proportion of 10 g of peptone, 10 g of beef extract, 5 g of yeast extract, 2 g of dipotassium hydrogen phosphate, 2 g of diammonium citrate, 5 g of sodium acetate, 20 g of glucose, 1 mL of Tween 80, 0.5 g of magnesium sulfate, 0.25 g of manganese sulfate, 1 g of pectic acid, and 1 g of succinic acid, make up the volume to 1 L in a volumetric flask, adjust the pH value to 6.2 - 6.4, and autoclave (101 Kpa, 121 °C) for 15 min to obtain the selenium-protecting liquid MRS medium.

[0044] (4)After activating the Lactobacillus plantarum CGMCC 1.3919, inoculate it into the selenium-protecting liquid MRS medium and statically cultivate it in liquid for 36 - 72 h until the turbidity OD 600 of the culture solution is between 3.0 - 3.5 to obtain the Lactobacillus plantarum fermentation broth.

[0045] (5)Evenly sprinkle the Lactobacillus plantarum fermentation broth prepared in step (4) on the blanched selenium-rich rape bolts and conduct aerobic fermentation at 37 ± 2 °C for 4 h to obtain the fermented selenium-rich rape bolts.

[0046] (6)Soak the selenium-rich rape bolts in water and put the rape bolts into an oven and treat them at 80 °C for 1 min to quickly kill the residual Lactobacillus plantarum on the surface of the selenium-rich rape bolts. Then, adjust the temperature to 50 °C and continue drying to obtain the selenium-rich dehydrated rape bolts.

[0047] (7)Crush the dried selenium-rich dehydrated rape bolts, mix them with water according to the solid-liquid ratio of 1:4 (m / V), add 4% sucrose according to the mass-volume ratio, homogenize and mix, then inoculate 2% Saccharomyces cerevisiae according to the mass-volume ratio, and ferment at 28 °C for 6 h. After fermentation, sterilize and dry to a water content of 8% to obtain the selenium-rich rape bolts rich in plant active selenium.

[0048] Example 3

[0049] This example provides a method for increasing the content of plant active selenium in selenium-rich rape bolts. The specific method is as follows.

[0050] (1)Prepare a mixed blanching protective solution. In the blanching protective solution, the content of CaCl2 is 0.01%.

[0051] (2)Control the water temperature in the fruit and vegetable blanching machine between 80 - 90 °C. Then put the selenium-rich rape stems that are of the same length, fresh, free from pests and diseases, and mechanical damage into the blanching protective solution and blanch them in the blanching pot of the fruit and vegetable blanching machine for 30 - 60 s, ensuring that the selenium-rich rape stems are evenly heated during the blanching process. After blanching, transfer the selenium-rich rape stems to the cooling pot of the fruit and vegetable blanching machine and cool them by the bubble water cooling method. After cooling for 2 - 5 min, take out the selenium-rich rape stems to obtain the blanched selenium-rich rape stems.

[0052] (3)Preparation of selenium-protecting liquid MRS medium: According to the proportion of 10 g of peptone, 10 g of beef extract, 5 g of yeast extract, 2 g of dipotassium hydrogen phosphate, 2 g of diammonium citrate, 5 g of sodium acetate, 20 g of glucose, 1 mL of Tween 80, 0.5 g of magnesium sulfate, 0.25 g of manganese sulfate, 10 g of pectic acid, and 10 g of succinic acid, make up the volume to 1 L in a volumetric flask, adjust the pH value to 6.2 - 6.4, and autoclave (101 Kpa, 121 °C) for 15 min to obtain the selenium-protecting liquid MRS medium.

[0053] (4)After activating Lactobacillus plantarum CGMCC 1.3919, inoculate it into the selenium-protecting liquid MRS medium and statically cultivate it in liquid for 36 - 72 h until the turbidity OD 600 is between 3.0 - 3.5 to obtain the Lactobacillus plantarum fermentation broth.

[0054] (5)Evenly sprinkle the Lactobacillus plantarum fermentation broth prepared in step (4) on the blanched selenium-rich rape stems and aerobically ferment them at 37 ± 2 °C for 24 h to obtain the fermented selenium-rich rape stems.

[0055] (6)Soak the selenium-rich rape stems in water and put the rape stems into an oven and treat them at 70 °C for 3 min to quickly kill the remaining Lactobacillus plantarum on the surface of the selenium-rich rape stems. Then, adjust the temperature to 40 °C and dry them at 40 °C to obtain selenium-rich dehydrated rape stems.

[0056] (7)Crush the dried selenium-rich dehydrated rape stems, mix them with water according to the solid-liquid ratio of 1:1 (m / V), add 0.3% glucose according to the mass-volume ratio, homogenize and mix, then inoculate 0.2% Saccharomyces cerevisiae according to the mass-volume ratio, and ferment at 25 °C for 6 h. After fermentation, sterilize to obtain selenium-rich rape stems rich in plant active selenium.

[0057] Example 4

[0058] This example provides a method for preparing selenium-rich rape stem fruit and vegetable juice rich in plant active selenium, and the specific method is as follows.

[0059] (1) Prepare a mixed blanching protective solution. In the blanching protective solution, the content of CaCl2 is 0.05%.

[0060] (2) Control the water temperature in the fruit and vegetable blanching machine between 80 - 90 °C. Then put the selenium-rich rape bolts that are of the same length, tender, free of pests and diseases, and free of mechanical damage into the blanching protective solution, and blanch them in the blanching pot of the fruit and vegetable blanching machine for 30 - 60 s to ensure uniform heating of the selenium-rich rape bolts during the blanching process. After blanching, transfer the selenium-rich rape bolts to the cooling pot of the fruit and vegetable blanching machine, and use the bubble water cooling method. After cooling for 2 - 5 min, take out the selenium-rich rape bolts to obtain the blanched selenium-rich rape bolts.

[0061] (3) Preparation of selenium-protecting liquid MRS medium: Weigh 10 g of peptone, 10 g of beef extract, 5 g of yeast extract, 2 g of dipotassium hydrogen phosphate, 2 g of diammonium citrate, 5 g of sodium acetate, 20 g of glucose, 1 mL of Tween 80, 0.5 g of magnesium sulfate, 0.25 g of manganese sulfate, 4 g of pectic acid, and 2 g of succinic acid, and make up the volume to 1 L in a volumetric flask. Adjust the pH value to 6.2 - 6.4, and autoclave (101 Kpa, 121 °C) for 15 min to obtain the selenium-protecting liquid MRS medium.

[0062] (4) Activate Leuconostoc mesenteroides subsp. mesenteroides CGMCC 1.20 and inoculate it into the selenium-protecting liquid MRS medium, and statically culture it in liquid for 36 - 72 h until the turbidity OD 600 of the culture solution is between 3.0 - 3.5 to obtain the fermentation broth of Leuconostoc mesenteroides subsp. mesenteroides.

[0063] (5) Evenly sprinkle the fermentation broth of Leuconostoc mesenteroides subsp. mesenteroides prepared in step (4) on the blanched selenium-rich rape bolts, and carry out aerobic fermentation at 37 ± 2 °C for 18 h to obtain the fermented selenium-rich rape bolts.

[0064] (6) Soak the selenium-rich rape bolts in water, and put the rape bolts into an oven and treat them at 72 °C for 2 min to quickly kill the remaining Leuconostoc mesenteroides subsp. mesenteroides on the surface of the selenium-rich rape bolts. Then, adjust the temperature to 48 °C and dry them at 48 °C to obtain selenium-rich dehydrated rape bolts.

[0065] (7) Crush the dried selenium-rich dehydrated rape bolts, mix them with water according to the solid-liquid ratio of 1:3 (m / V), add 1% glucose according to the mass-volume ratio, homogenize and mix, then inoculate 0.5% Angel yeast according to the mass-volume ratio, and ferment at 28 °C for 3 h. After fermentation, carry out high-pressure sterilization in a clockwise direction at 121 °C to obtain selenium-rich rape bolts rich in plant active selenium.

[0066] (8) Place selenium-rich rape bolts containing plant active selenium into a fruit and vegetable juicer, add water to juice, and heat the obtained rape bolt juice at 70 °C, adjust the sugar-acid ratio, and mix it with concentrated orange juice, apple juice, or carrot juice, etc., to obtain a mixed fruit and vegetable juice rich in plant active selenium.

[0067] . After testing, the content of plant active selenium in rape bolts treated by this method increased by 28.85 ± 1.18% compared with those untreated.

Claims

1. A method for increasing the content of plant active selenium in selenium rape shoots, characterized in that: The method for increasing the content of plant active selenium in selenium rape bolts comprises the following steps: ① Heat blanch the fresh selenium rape bolts with an aqueous solution containing CaCl2; ② Inoculate Leuconostoc mesenteroides subsp. mesenteroides or Lactobacillus plantarum into an MRS medium containing pectic acid and succinic acid and culture for 36 - 72 h. Then evenly sprinkle the bacterial liquid on the heat-blanched selenium rape bolts and perform aerobic fermentation at 37 ± 2 °C for 4 - 24 h to obtain fermented selenium rape bolts. The preservation number of Leuconostoc mesenteroides subsp. mesenteroides is: CGMCC 1.20, and the preservation number of Lactobacillus plantarum is: CGMCC 1.3919. The mass-volume fraction of pectic acid and succinic acid is 0.1 - 1% of the final concentration of the whole medium; ③ After heat treatment at 70 - 80 °C for 1 - 3 min, adjust the temperature to 40 - 50 °C and dry until the moisture content is less than 8%; ④ Add water, fermentable carbon source and yeast to the pulverized and dried selenium-rich dehydrated rape bolts, place them at 25 - 30 °C for fermentation for 3 - 6 h, and perform sterilization treatment after fermentation is completed to obtain rape bolts rich in plant active selenium.

2. The method for increasing the content of plant active selenium in selenium rape stems according to claim 1, wherein: The concentration of the aqueous solution of CaCl2 is 0.01 - 0.1%.

3. The method for increasing the content of plant active selenium in selenium rape bolts according to claim 1, characterized in that: The fermentable carbon source includes sucrose and glucose.

4. The method for increasing the content of plant active selenium in selenium rape bolts according to claim 1, characterized in that: The mass-volume ratio of the dried selenium-rich dehydrated rape bolts to water in the mixture is 1:1 - 1:

4.

5. The method for increasing the content of plant active selenium in selenium rape bolts according to claim 1, characterized in that: The addition amount of the yeast is 0.2 - 2%, and the addition amount of the fermentable carbon source is 0.3 - 4%.

6. The method for increasing the content of plant active selenium in selenium rape shoots according to claim 1, characterized in that: The yeast is Angel yeast or Saccharomyces cerevisiae.

7. Use of the method for increasing the content of plant active selenium in selenium rape stems according to claim 1, characterized in that: It is used for preparing rape bolt fruit and vegetable juice rich in plant active selenium.

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