Mri traceable cell microcarriers and methods of making same

By preparing MRI-tracing cell microcarriers that combine γ-Fe2O3 nanoparticles with silk fibroin, the problem of poor tracking effect in existing technologies has been solved, enabling precise in vivo localization and targeted therapy, and improving the survival rate and therapeutic effect of stem cells.

CN115998909BActive Publication Date: 2025-12-30SHANXI UNIV OF CHINESE MEDICINE
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Patent Information

Application Number
CN202310054972.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-02-03
Publication Date
2025-12-30
Estimated Expiration
2043-02-03

AI Technical Summary

Technical Problem

Existing microcarriers have poor in vivo tracking performance, and biofluorescent markers have drawbacks such as low fluorescence intensity and easy quenching, which affect the efficacy of stem cell therapy.

Method used

By combining γ-Fe2O3 nanoparticles with silk fibroin and cross-linking them through hydrogen bonds to form MRI-traceable cell microcarriers, and utilizing the superparamagnetism of γ-Fe2O3 and the biocompatibility of silk fibroin, combined with baicalin as a therapeutic drug, cell microcarriers that can be precisely located and traced in vivo were prepared.

Benefits of technology

It achieves good imaging and targeted therapeutic effects in vivo, improves the survival rate and therapeutic effect of stem cells, and protects neuronal function, especially in stroke.

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Abstract

The application relates to the technical field of biology, and particularly discloses a cell microcarrier capable of MRI tracing and a preparation method thereof. The preparation method of the cell microcarrier capable of MRI tracing comprises the following steps: in step one, gamma-Fe2O3 nanoparticles and water are uniformly mixed through ultrasonic mixing, silk fibroin is added and uniformly stirred, then a baicalin solution and castor oil are added and uniformly mixed and stirred, ultrasonic emulsification is carried out at 3-5 DEG C to obtain a mixed emulsion; in step two, the mixed emulsion prepared in step one is added drop by drop into castor oil, stirred for 20-35 min, then cooled, anhydrous ethanol is added, extraction is carried out, centrifugal separation is carried out, and drying is carried out to obtain the cell microcarrier. The cell microcarrier prepared in the application can be used for supporting stem cells to exert better therapeutic effects in vivo.
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