A method for producing PHB by fermentation of Corynebacterium glutamicum

CN116004737BActive Publication Date: 2026-03-13CATHAY BIOTECH INC +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2021-10-19
Publication Date
2026-03-13

AI Technical Summary

Technical Problem

Existing Corynebacterium glutamicum strains used as PHB-producing strains suffer from genetic instability and insufficient yield, making it difficult to effectively modify them by expressing PHB synthesis-related genes via plasmids.

Method used

The PHB synthesis genes PhaA, PhaB, and PhaC were integrated into the genome of Corynebacterium glutamicum via gene integration. The carbon-nitrogen ratio, pH value, and feeding strategy during fermentation were optimized, including controlling the carbon-nitrogen ratio at (17–53):1, preferably (23–24):1, the fermentation temperature at 20–40℃, the stirring speed at 400–800 rpm, the aeration rate at 0.3–3.0 vvm, the gradual addition of nitrogen sources such as ammonium sulfate during fermentation, and monitoring and controlling the pH value at 6.0–8.0.

Benefits of technology

It significantly increased the PHB yield of Corynebacterium glutamicum, achieving stable and efficient production.

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Abstract

This invention discloses a method for producing PHB by fermentation of Corynebacterium glutamicum. The method includes controlling the carbon-nitrogen molar ratio in the fermentation medium to (17-53):1, preferably (20-28):1, during the fermentation process by feeding supplements. This invention improves the fermentation yield of PHB by adjusting the carbon-nitrogen ratio in the fermentation medium to influence the fermentation process of PHB production by Corynebacterium glutamicum.
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Citation Information

Patent Citations

  • Construction method of synthetic path for generating glutamic acid by utilizing xylose in corynebacterium glutamicum

    CN112458108A