Dehydrogenase mutant, gene, engineering bacterium and application
By directionally modifying key sites of Nocardia dehydrogenase, a highly active and specific dehydrogenase mutant was constructed, solving the problems of low conversion rate and slow reaction rate in the dehydrogenation reaction of anecoxal acetate, and realizing the efficient and environmentally friendly preparation of anecoxal acetate dehydrogenates.
Patent Information
- Application Number
- CN202610011045.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-06
- Publication Date
- 2026-04-03
AI Technical Summary
Existing technologies for the dehydrogenation of acetic acid anocorta suffer from low conversion rates, slow reaction rates, and numerous side reactions. In particular, when using Nocardia, problems such as foaming and contamination by other microorganisms arise. Furthermore, traditional methods require emulsification with organic solvents, which leads to significant environmental pressure.
By directionally modifying KstD dehydrogenase from Nocardia and mutating its key sites, a highly active and specific dehydrogenase mutant was constructed for the preparation of anocorta acetate dehydrogenase, avoiding the use of organic solvents and simplifying the process.
It achieves high conversion rate (over 98.5%) and rapid reaction (no more than 28 hours) of arnecrostat dehydrogenase, with no hydrolysis side reaction, simplifying the process and reducing environmental pressure.