An enzyme immunoassay analyzer
By setting up additional components and guide rails inside the shell of the enzyme immunoassay device, the full mechanical operation is achieved, and the problem of insufficient accuracy in the concentration, reaction time and reagent addition during the enzyme immunoassay process in the prior art is solved, and the accuracy of experimental data and the safety of reagents are improved.
Patent Information
- Application Number
- CN202310214381.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-02-28
- Publication Date
- 2025-06-03
- Estimated Expiration
- 2043-02-28
AI Technical Summary
The existing enzyme immunoassays have problems with insufficient concentration, reaction time and reagent addition during the enzyme immunotherapy process, which leads to excessive gap in experimental data and the external operating environment is prone to contamination of reagents.
An enzyme immunoassay device was designed to achieve full mechanical operation by setting up additional components and guide rails inside the housing. The addition components are programmed to absorb appropriate amounts of reagent from different raw material tubes, and drive the moving disk and kit to move to the detection module to detect the absorbance value.
By placing all the pretreatment process of the enzyme immunoassay process inside the shell, the whole process is mechanically operated, which reduces artificial errors, improves data accuracy, and reduces the risk of reagent contamination.
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Figure CN116068168B_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the field of enzyme-linked immunosorbent assay, and specifically relates to an enzyme immunoassay analyzer. Background Art
[0002] Enzyme-linked immunosorbent assay, abbreviated as enzyme immunoassay, uses the principle of antibody-enzyme complex binding and then detects by color development. It is widely used in clinical diagnosis of hospitals at all levels, microbiology, immunology, pathology, biology, pharmacology, animal husbandry, and agricultural science and other fields.
[0003] Analytical instruments for enzyme immunoassay are commonly found in laboratories. They use optical elements inside to detect the light absorption of the samples placed and record the data, and then obtain the chemical properties of the samples through later data analysis.
[0004] Existing enzyme immunoassay analyzers are often used in the final detection stage. The products that have completed processing, cultivation, and reaction are directly detected for their light absorption values through the detection module. However, the enzyme immunoassay process takes a long time and is generally manually operated by researchers, which easily causes inaccuracies in concentration, reaction time, and the amount of reagent added, resulting in large differences in subsequent experimental data, and the external operating environment is also likely to contaminate the reagents.
[0005] Therefore, the present invention provides an enzyme immunoassay analyzer. Summary of the Invention
[0006] In order to make up for the deficiencies of the existing technology and solve at least one of the technical problems proposed in the background art.
[0007] The technical solution adopted by the present invention to solve its technical problems is as follows: An enzyme immunoassay analyzer of the present invention includes a housing. The inside of the housing is hollow. A second flip cover is rotatably connected to the front end of the housing. Two symmetrically arranged guide rails are fixedly connected to the inside of the housing. A moving disk is slidably connected between the two guide rails. Two electric wheels are fixedly connected to one end of the moving disk away from the second flip cover. A reagent kit is arranged on the top surface of the moving disk. A detection module is fixedly connected to the top end inside the housing. A display screen is fixedly connected to the top of the housing. The display screen is communicatively connected to the detection module. An adding component is arranged inside the housing. The adding component is used to add reaction reagents to the reagent kit. Multiple groups of raw material tubes are arranged on one side of the guide rail. The adding component is set by a program to suck an appropriate amount of reagent from different raw material tubes each time and put it into the reagent kit for cultivation. After the cultivation is completed, the moving disk and the reagent kit are driven by the guide rail and the electric wheels to move under the detection module to detect the light absorption value. Through this setting, the pretreatment process of the enzyme immunoassay analysis process is all placed inside the housing, and the whole process is mechanically operated, which not only facilitates the overall analysis process, but also makes the data more accurate and reduces the errors caused by humans.
[0008] Preferably, the adding component includes a moving seat, the top end of the moving seat is fixedly connected with an electric telescopic rod, the bottom end of the moving seat is fixedly connected with a plurality of absorption tubes, the inner side of the moving seat is fixedly connected with a negative pressure pump and a hair dryer, both the negative pressure pump and the hair dryer are communicated with the absorption tubes, the top end of the moving seat is fixedly connected with a moving module, the inner top end of the housing is fixedly connected with a moving track, the moving module is slidably clamped with the moving track, the moving seat is driven to move by the moving module, the lifting of the moving seat is controlled by the electric telescopic rod, the raw materials in the raw material tube are equally absorbed by the negative pressure generated by the negative pressure pump and transferred to the reagent kit. Through this setting, the effect of equally adding raw materials is achieved.
[0009] Preferably, a raw material tray is fixedly connected to one side of the guide rail, a plurality of holes are formed in the top surface of the raw material tray, a plurality of rubber tubes are placed in the holes, the rubber tubes are arranged in a shape with a wider top and a narrower bottom, and the rubber tubes are vertically permeable. Through this setting, only by pre-placing rubber tubes with the same number as the number of experimental groups, the same number of groups of raw materials can be accurately absorbed without excessive absorption, and the raw materials will be sucked into the rubber tubes without touching the absorption tubes, so that the raw materials will not be contaminated with each other through the absorption tubes. After one use, the rubber tubes are blown off by the hair dryer in the moving seat, and new rubber tubes are taken.
[0010] Preferably, the raw material tube includes a fixed sleeve, the fixed sleeve is fixedly connected with the housing, the top surface of the fixed sleeve is open, one end of the fixed sleeve passes through the housing and leaks outside, an irregular test tube is arranged inside the fixed sleeve, and a through hole is formed in the outer side of the irregular test tube. The fixed sleeve and the irregular test tube are separately arranged, which is convenient for the taking and replacement of the irregular test tube. The setting of the outer through hole allows the rubber tube to be inserted from the outside of the lying irregular test tube and absorb the raw materials.
[0011] Preferably, an anti-misoperation ring is fixedly connected to the connection part between the fixed sleeve and the housing, a notch is formed at the top end of the anti-misoperation ring, a docking plug is fixedly connected to the end of the irregular test tube, a one-way piece is arranged at the through hole of the irregular test tube, the one-way piece is located inside the irregular test tube, the one-way piece is made of an elastic material, and one long side of the one-way piece is fixedly connected with the inner wall of the irregular test tube. Through this setting, not only the volatilization of the raw materials is reduced, but also when the irregular test tube is taken out, it can be normally erected without liquid leakage. At the same time, when the rubber tube presses down the one-way piece, the bonding force between the rubber tube and the absorption tube can also be strengthened.
[0012] Preferably, a flip cover one is rotatably connected to one side of the housing, and a recovery drawer is slidably connected to the inner side of the housing. The recovery drawer is located at the rear end of the inner side of the housing. After the rubber tube is used up, the moving seat is moved onto the recovery drawer, and the hair dryer is started to blow the rubber tube into the recovery drawer, thus achieving the effect of recovering the rubber tube.
[0013] Preferably, the middle part of the movable disk is arranged in the air. A rotating disk is arranged in the middle of the movable disk. One end of the rotating disk is rotatably connected to the movable disk, and the other end of the rotating disk is fixedly connected to a servo motor. The output end of the servo motor is fixedly connected to the movable disk. A water absorption pad is arranged below the movable disk. The whole movable disk is driven by an electric wheel to move, replacing the shaking effect, so as to remove excess residual liquid. Through this setting, the operating system of the device is more comprehensive. The setting of the water absorption pad prevents the residual liquid from polluting the device.
[0014] Preferably, two insertion grooves are formed in the inner wall of the movable disk. Spring rods are fixedly connected to both ends of the reagent kit. The spring rod is composed of a telescopic rod and a spring. The reagent kit has only two states, upward and downward. In cooperation with the setting of the spring rod, whenever the reagent kit rotates upward or downward, the spring rod will be squeezed and then pop out, so as to enter the insertion groove to ensure the stable state of the reagent kit.
[0015] Preferably, two rotating wheels are rotatably connected to the inner side of the outer shell. The two rotating wheels are arranged in parallel. The two ends of the water absorption pad are respectively rotatably connected to the ends of the two rotating wheels. A plurality of friction pads are fixedly connected to the outer side of the spring rod. A transmission belt is sleeved between the two rotating wheels. Two expansion frames are also rotatably connected to the inner side of the outer shell. The two expansion frames are in active contact with the bottom surface of the water absorption pad. Through this setting, not only the continuous renewal of the water absorption pad is realized, but also no new power source is required for the device and no excess energy is consumed.
[0016] Preferably, a sealing pad is arranged between the detection module and the reagent kit. A connecting frame is arranged on the inner side of the outer shell. A lifting module is fixedly connected between the connecting frame and the outer shell. The connecting frame includes four cross bars. The sealing pad is fixedly connected to the outer sides of two of the cross bars. A driving motor is fixedly connected to the outer side of one of the cross bars. An extension rod is fixedly connected between the driving motor and the outer shell. Two suction fans are fixedly connected to the bottom surface of the rotating disk. Two air suction holes are formed in the top surface of the rotating disk. The air suction holes are communicated with the suction fans. An adsorption slit penetrating from the top surface to the bottom surface is formed at the edge of the top surface of the reagent kit, so that the reagent in the reagent kit can be isolated from the outside for accurate reaction, further improving the accuracy of the data.
[0017] The beneficial effects of the present invention are as follows:
[0018] 1. For the enzyme immunoassay analyzer of the present invention, through the setting of the adding component, the adding component is set by a program. Each time, an appropriate amount of reagent is sucked from different raw material tubes and put into the reagent kit for cultivation. After the cultivation is completed, the movable disk and the reagent kit are driven by a guide rail and an electric wheel to move under the detection module to detect the absorbance value. Through this setting, the pretreatment process of the enzyme immunoassay process is all placed inside the outer shell, and the whole process is mechanically operated, which not only facilitates the overall analysis process, but also makes the data more accurate and reduces the error caused by humans.
[0019] 2. The enzyme immunoassay analyzer of the present invention, through the settings of the raw material tray and the rubber tube, before absorbing the raw materials, the moving seat is first moved above the raw material tray. As the moving seat descends, the absorption tube will insert into the top of the rubber tube. With its upper-wide and lower-narrow setting, the rubber tube will temporarily sleeve under the absorption tube. After having the rubber tube, the bottom of the absorption tube will grow a section, so that the raw materials can be normally absorbed. Without the rubber tube, the height is not enough to absorb the raw materials. Through this setting, only need to pre-place the same number of rubber tubes as the number of experimental groups, and the raw materials of the same number of groups can be accurately absorbed, without redundant absorption, and the raw materials will be sucked into the rubber tube and will not touch the absorption tube, so that the raw materials will not be contaminated with each other through the absorption tube. When the use is over once, the rubber tube is blown off through the hair dryer in the moving seat, and a new rubber tube is taken. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] The present invention will be further described below in conjunction with the accompanying drawings.
[0021] Figure 1 is the three-dimensional view of the present invention;
[0022] Figure 2 is the cross-sectional view of the present invention;
[0023] Figure 3 is the three-dimensional view of the guide rail and the reagent kit in the present invention;
[0024] Figure 4 is the three-dimensional view of the moving disk and the reagent kit in the present invention;
[0025] Figure 5 is the three-dimensional view of the raw material tube in the present invention;
[0026] Figure 6 is the three-dimensional view of the sealing pad and the water absorption pad in the present invention;
[0027] Figure 7 is the three-dimensional view of the moving seat in the present invention;
[0028] In the figure: 1, outer shell; 2, first flip cover; 3, second flip cover; 4, display screen; 5, raw material tube; 6, raw material tray; 7, recycling drawer; 8, moving seat; 9, detection module; 10, sealing pad; 11, reagent kit; 12, guide rail; 14, moving disk; 15, electric wheel; 16, water absorption pad; 17, transmission belt; 18, rotating wheel; 19, spring rod; 20, friction pad; 21, rotating disk; 22, air suction hole; 23, fixed sleeve; 24, anti-fooling ring; 25, special-shaped test tube; 26, one-way piece; 27, adsorption slit; 28, connecting frame; 29, expansion frame; 30, electric telescopic rod; 31, moving module; 32, absorption tube. DETAILED DESCRIPTION OF THE INVENTION
[0029] In order to make the technical means, creative features, achieved purposes and effects realized by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.
[0030] Embodiment 1
[0031] As Figures 1 to 3 shown, an enzyme immunoassay analyzer according to an embodiment of the present invention includes a housing 1, the inside of the housing 1 is hollow, a second flip cover 3 is rotatably connected to the front end of the housing 1, two symmetrically arranged guide rails 12 are fixedly connected inside the housing 1, a moving disk 14 is slidably connected between the two guide rails 12, two electric wheels 15 are fixedly connected to one end of the moving disk 14 away from the second flip cover 3, a reagent kit 11 is arranged on the top surface of the moving disk 14, a detection module 9 is fixedly connected to the top end inside the housing 1, a display screen 4 is fixedly connected to the top end of the housing 1, the display screen 4 is communicatively connected to the detection module 9, an adding component is arranged inside the housing 1, and the adding component is used to add reaction reagents to the reagent kit 11. A plurality of raw material tubes 5 are arranged on one side of the guide rail 12. During operation, existing enzyme immunoassay analyzers are often used in the final detection stage to directly detect the absorbance value of products that have been processed, cultured and reacted through the detection module 9. However, the enzyme immunoassay process takes a long time and is generally manually operated by scientific researchers, which is likely to cause inaccuracies in concentration, reaction time, and added reagent dosage, resulting in large differences in subsequent experimental data. Moreover, the external operating environment is also likely to contaminate the reagents. Through the setting of the adding component, the adding component is set by a program to absorb an appropriate amount of reagent from different raw material tubes 5 into the reagent kit 11 for culturing each time. After the culturing is completed, the moving disk 14 and the reagent kit 11 are driven by the guide rail 12 and the electric wheels 15 to move under the detection module 9 to detect the absorbance value. Through this setting, the pretreatment process of the enzyme immunoassay process is all placed inside the housing 1, and the whole process is mechanically operated, which not only facilitates the overall analysis process, but also makes the data more accurate and reduces the errors caused by humans.
[0032] As Figure 7 shown, the adding component includes a moving seat 8, an electric telescopic rod 30 is fixedly connected to the top end of the moving seat 8, a plurality of absorption tubes 32 are fixedly connected to the bottom end of the moving seat 8, a negative pressure pump and a blower are fixedly connected inside the moving seat 8, the negative pressure pump and the blower are both communicated with the absorption tubes 32, a moving module 31 is fixedly connected to the top end of the moving seat 8, a moving rail is fixedly connected to the top end inside the housing 1, and the moving module 31 is slidably clamped with the moving rail. During operation, the moving seat 8 is driven by the moving module 31, the lifting of the moving seat 8 is controlled by the electric telescopic rod 30, and the raw materials in the raw material tube 5 are equally absorbed by the negative pressure generated by the negative pressure pump and transferred to the reagent kit 11. Through this setting, the effect of equally adding raw materials is achieved.
[0033] AsFigures 2 to 3 As shown, a raw material tray 6 is fixedly connected to one side of the guide rail 12. A plurality of holes are formed in the top surface of the raw material tray 6, and a plurality of rubber tubes are placed in the holes. The rubber tubes are arranged in a shape with a wider top and a narrower bottom, and the rubber tubes are vertically permeable. During work, in the process of enzyme immunoassay, generally a blank control group and multiple concentration gradient groups are required as experimental references. However, the number of control groups used in each experiment is different, while the number of absorption tubes 32 is fixed, making it difficult to accurately match. Through the setting of the raw material tray 6 and the rubber tubes, before absorbing the raw materials, first move the moving seat 8 above the raw material tray 6. As the moving seat 8 descends, the absorption tube 32 will insert into the top of the rubber tube. With its wider top and narrower bottom setting, the rubber tube will temporarily sleeve under the absorption tube 32. With the rubber tube, the bottom of the absorption tube 32 will grow a section, so that the raw materials can be normally absorbed. Without the rubber tube, the height is not enough to absorb the raw materials. Through this setting, just pre-place the same number of rubber tubes as the number of experimental groups, and the raw materials of the same number of groups can be accurately absorbed without excess absorption. Moreover, the raw materials will be sucked into the rubber tubes and will not touch the absorption tube 32, so that the raw materials will not be contaminated with each other through the absorption tube 32. When one use is over, the rubber tube is blown off by the hair dryer in the moving seat 8, and a new rubber tube is taken.
[0034] As Figures 2 to 5 As shown, the raw material tube 5 includes a fixed sleeve 23. The fixed sleeve 23 is fixedly connected to the outer shell 1. The top surface of the fixed sleeve 23 is open. One end of the fixed sleeve 23 passes through the outer shell 1 and leaks outside. An irregular test tube 25 is arranged inside the fixed sleeve 23, and a through hole is formed in the outer side of the irregular test tube 25. During work, the fixed sleeve 23 and the irregular test tube 25 are separately arranged, which is convenient for taking and replacing the irregular test tube 25. With the through hole on the outer side, the rubber tube can be inserted from the outside of the lying irregular test tube 25 and absorb the raw materials.
[0035] As Figures 2 to 5As shown, a foolproof ring 24 is fixedly connected to the connection part between the fixed sleeve 23 and the outer shell 1. A notch is provided at the top of the foolproof ring 24. A docking plug is fixedly connected to the end of the special-shaped test tube 25. A one-way piece 26 is arranged at the through port of the special-shaped test tube 25. The one-way piece 26 is located inside the special-shaped test tube 25. The one-way piece 26 is made of an elastic material. One long side of the one-way piece 26 is fixedly connected to the inner wall of the special-shaped test tube 25. During operation, the raw materials are prone to volatilization when left open for a long time. Through the setting of the one-way piece 26, when the rubber tube sinks, it will push the one-way piece 26 downward. With its elastic material and the setting of single-side fixation, the one-way piece 26 is pressed down and bent, allowing the rubber tube to enter normally. When the external pressure disappears, the one-way piece 26 will rebound under its own elastic force and thus close. Through this setting, not only is the volatilization of raw materials reduced, but also when the special-shaped test tube 25 is taken out, it can stand upright normally without leakage. At the same time, when the rubber tube presses down the one-way piece 26, the bonding force between the rubber tube and the absorption tube 32 can also be strengthened.
[0036] As Figures 1 to 2 shown, a flip cover one 2 is rotatably connected to one side of the outer shell 1. A recycling drawer 7 is slidably connected to the inner side of the outer shell 1. The recycling drawer 7 is located at the rear end inside the outer shell 1. During operation, when the rubber tube is used up, the moving seat 8 is moved onto the recycling drawer 7, and the hair dryer is started to blow the rubber tube into the recycling drawer 7, thus achieving the effect of recycling the rubber tube.
[0037] As Figures 3 to 4 shown, the middle part of the moving disk 14 is arranged in the air. A rotating disk 21 is arranged in the middle of the moving disk 14. One end of the rotating disk 21 is rotatably connected to the moving disk 14. The other end of the rotating disk 21 is fixedly connected to a servo motor. The output end of the servo motor is fixedly connected to the moving disk 14. A water absorption pad 16 is arranged below the moving disk 14. During operation, in the process of enzyme immunoassay, every time a raw material is added, after the reaction time, it is necessary to remove the excess stock solution. Manual operation is generally to turn over the reagent kit 11 and place a water absorption pad 16 below, and remove the excess liquid by shaking the reagent kit 11. Initially, the base has high viscosity and the base will not fall off during shaking. In this device, the servo motor drives the rotating disk 21 to rotate, making the reagent kit 11 face downward. Then, the electric wheel 15 drives the entire moving disk 14 to move, replacing the shaking effect, so as to remove the excess residual liquid. Through this setting, the operating system of the device is more comprehensive. The setting of the water absorption pad 16 makes the residual liquid not pollute the device.
[0038] As Figures 3 to 4As shown in the figure, two insertion slots are provided on the inner wall of the moving disk 14. Both ends of the reagent kit 11 are fixedly connected with spring rods 19. The spring rods 19 are composed of telescopic rods and springs. During operation, the reagent kit 11 has only two states, upward and downward. With the arrangement of the spring rods 19, whenever the reagent kit 11 rotates upward or downward, the spring rods 19 will be squeezed and then ejected, so as to enter the insertion slots, ensuring the stable state of the reagent kit 11.
[0039] As Figures 3 to 6 shown in the figure, two rotating wheels 18 are rotatably connected to the inner side of the outer shell 1. The two rotating wheels 18 are arranged in parallel. Both ends of the water absorption pad 16 are respectively rotatably connected to the ends of the two rotating wheels 18. A plurality of friction pads 20 are fixedly connected to the outer side of the spring rod 19. A transmission belt 17 is sleeved between the two rotating wheels 18. Two expansion frames 29 are also rotatably connected to the inner side of the outer shell 1. The two expansion frames 29 are movably attached to the bottom surface of the water absorption pad 16. During operation, each time the reagent kit 11 is inverted on the water absorption pad 16, the water absorption pad 16 needs to be replaced with a new one to ensure the water absorption effect. Through the arrangement of the transmission belt 17, whenever the reagent kit 11 rotates, the reagent kit 11 will rub against the surface of the water absorption pad 16, thereby pushing the water absorption pad 16 to move, so that one of the rotating wheels 18 rotates to spit out a new water absorption pad 16, and the transmission belt 17 will drive the other rotating wheel 18 to rotate to wind up the water absorption pad 16. Through this setting, not only the continuous update of the water absorption pad 16 is realized, but also no new power source is required for the device and no extra energy is consumed.
[0040] Embodiment 2
[0041] As Figures 3 to 6 shown in the figure, compared with Embodiment 1, another implementation manner of the present invention is: a sealing pad 10 is arranged between the detection module 9 and the reagent kit 11. A connecting frame 28 is arranged on the inner side of the outer shell 1. A lifting module is fixedly connected between the connecting frame 28 and the outer shell 1. The connecting frame 28 includes four cross bars. The sealing pad 10 is fixedly connected to the outer sides of two of the cross bars. A driving motor is fixedly connected to the outer side of one of the cross bars. An extension rod is fixedly connected between the driving motor and the outer shell 1. Two suction fans are fixedly connected to the bottom surface of the rotating disk 21. Two air suction holes 22 are opened on the top surface of the rotating disk 21. The air suction holes 22 are communicated with the suction fans. An adsorption slit 27 penetrating from the top surface to the bottom surface is opened at the edge of the top surface of the reagent kit 11. During operation, when adding a new reagent to the reagent kit 11, it generally needs to react for 30 minutes to 1 hour. This process is preferably closed. Through the arrangement of the sealing pad 10, when a new reagent is added, the moving disk 14 is moved to below the sealing pad 10, and the lifting module drives the sealing pad 10 to descend as a whole and cover the top of the reagent kit 11. The suction fans are started to generate negative pressure, and the negative pressure sucks the sealing pad 10 through the adsorption slit 27, so that the reagent in the reagent kit 11 can be isolated from the outside for accurate reaction, further improving the accuracy of the data.
[0042] During operation, existing enzyme immunoassay analyzers are often used in the final detection stage to directly detect the absorbance value of products that have completed processing, culturing, and reaction through the detection module 9. However, the enzyme immunoassay process takes a long time and is generally manually operated by researchers, which easily leads to inaccurate concentration, reaction time, and reagent addition amount, resulting in a large gap in subsequent experimental data. Moreover, the external operating environment is likely to contaminate the reagents. Through the setting of the adding component, the adding component is set by a program to draw an appropriate amount of reagent from different raw material tubes 5 into the reagent kit 11 for culturing each time. After the culturing is completed, the moving disk 14 and the reagent kit 11 are driven by the guide rail 12 and the electric wheel 15 to move under the detection module 9 to detect the absorbance value. Through this setting, the pretreatment process of the enzyme immunoassay process is entirely placed inside the housing 1, and the whole process is mechanically operated, which not only facilitates the overall analysis process but also makes the data more accurate and reduces the errors caused by humans; the moving module 31 drives the moving seat 8 to move, the lifting of the moving seat 8 is controlled by the electric telescopic rod 30, and a negative pressure is generated by the negative pressure pump to absorb the raw materials in the raw material tube 5 equally and transfer them to the reagent kit 11. Through this setting, the effect of equally adding raw materials is achieved; in the enzyme immunoassay process, generally a blank control group and multiple concentration gradient groups are required as experimental references. However, the number of control groups used in each experiment is different, and the number of absorption tubes 32 is fixed, making it difficult to match precisely. Through the setting of the raw material disk 6 and the rubber tube, before absorbing the raw materials, first let the moving seat 8 move above the raw material disk 6. As the moving seat 8 descends, the absorption tube 32 will insert into the top of the rubber tube. With its upper-wide and lower-narrow setting, the rubber tube will temporarily sleeve under the absorption tube 32. After having the rubber tube, the bottom of the absorption tube 32 will grow a section, so that the raw materials can be normally absorbed. Without the rubber tube, the height is not enough to absorb the raw materials. Through this setting, only need to pre-place the same number of rubber tubes as the number of experimental groups, and the raw materials of the same number of groups can be accurately absorbed without excess absorption. Moreover, the raw materials will be inhaled into the rubber tube and will not touch the absorption tube 32, so that the raw materials will not be contaminated with each other through the absorption tube 32. When the use is over once, the rubber tube is blown off by the hair dryer in the moving seat 8, and a new rubber tube is taken; the fixed sleeve 23 and the special-shaped test tube 25 are separately set, which is convenient for the taking and replacement of the special-shaped test tube 25. The setting of the outer through hole allows the rubber tube to be inserted from the outside of the lying special-shaped test tube 25 and absorb the raw materials; the raw materials are easily volatilized when exposed for a long time. Through the setting of the one-way piece 26, when the rubber tube sinks, it will push the one-way piece 26 downward. With its elastic material and one-sided fixed setting, the one-way piece 26 is pressed and bent downward, so that the rubber tube can normally enter it. When the external pressure disappears, the one-way piece 26 will rebound under its own elastic force and close. Through this setting, not only the volatilization of the raw materials is reduced, but also when the special-shaped test tube 25 is taken out, it can be normally erected without leakage. At the same time, when the rubber tube presses the one-way piece 26, the bonding force between the rubber tube and the absorption tube 32 can also be strengthened;After the use of the rubber hose is completed, move the movable seat 8 onto the recycling drawer 7, and start the hair dryer to blow the rubber hose into the recycling drawer 7, thus achieving the effect of recycling the rubber hose; during the enzyme immunoassay process, every time a raw material is added, after the reaction time, it is necessary to remove the excess stock solution. Manual operation is generally to turn the reagent kit 11 over and place an absorbent pad 16 below, and remove the excess liquid by shaking the reagent kit 11. Initially, the base has a high viscosity and the base will not fall off during shaking. In this device, the rotating disk 21 is driven by a servo motor to rotate, making the reagent kit 11 face downwards, and then the moving disk 14 is driven by the electric wheel 15 to move as a whole, replacing the shaking effect, thereby removing the excess residual liquid. Through this setting, the operating system of the device is made more comprehensive, and the setting of the absorbent pad 16 prevents the residual liquid from contaminating the device; the reagent kit 11 has only two states, up and down. With the setting of the spring rod 19, every time the reagent kit 11 rotates to the up or down position, the spring rod 19 will be squeezed and then pop out, thus entering the insertion slot to ensure the stable state of the reagent kit 11; every time it is inverted on the absorbent pad 16, the absorbent pad 16 needs to be replaced with a new one to ensure the water absorption effect. Through the setting of the transmission belt 17, every time the reagent kit 11 rotates, the reagent kit 11 will rub against the surface of the absorbent pad 16, thereby pushing the absorbent pad 16 to move, causing one of the rotating wheels 18 to rotate and spit out a new absorbent pad 16, and the transmission belt 17 will drive the other rotating wheel 18 to rotate and wind up the absorbent pad 16. Through this setting, not only the continuous update of the absorbent pad 16 is achieved, but also no new power source for the device is required, nor is any excess energy consumed.
[0043] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.
Claims
1. An enzyme immunoassay analyzer, characterized in that: it includes a housing (1), the inside of the housing (1) is hollow, a second flip cover (3) is rotatably connected to the front end of the housing (1), two symmetrically arranged guide rails (12) are fixedly connected to the inside of the housing (1), a moving disk (14) is slidably connected between the two guide rails (12), two electric wheels (15) are fixedly connected to one end of the moving disk (14) away from the second flip cover (3), a reagent kit (11) is arranged on the top surface of the moving disk (14), a detection module (9) is fixedly connected to the top end inside the housing (1), a display screen (4) is fixedly connected to the top end of the housing (1), the display screen (4) is communicatively connected to the detection module (9), an adding component is arranged inside the housing (1), the adding component is used to add reaction reagents to the reagent kit (11), and multiple groups of raw material tubes (5) are arranged on one side of the guide rail (12); the adding component includes a moving seat (8), an electric telescopic rod (30) is fixedly connected to the top end of the moving seat (8), multiple suction tubes (32) are fixedly connected to the bottom end of the moving seat (8), a negative pressure pump and a blower are fixedly connected to the inside of the moving seat (8), the negative pressure pump and the blower are both communicated with the suction tubes (32), a moving module (31) is fixedly connected to the top end of the moving seat (8), a moving rail is fixedly connected to the top end inside the housing (1), and the moving module (31) is slidably clamped with the moving rail; a raw material disk (6) is fixedly connected to one side of the guide rail (12), multiple holes are formed in the top surface of the raw material disk (6), multiple rubber tubes are placed in the holes, the rubber tubes are arranged in a shape with a wider top and a narrower bottom, and the rubber tubes are vertically through; the raw material tube (5) includes a fixing sleeve (23), the fixing sleeve (23) is fixedly connected to the housing (1), the top surface of the fixing sleeve (23) is open, one end of the fixing sleeve (23) passes through the housing (1) and leaks outside, a special-shaped test tube (25) is arranged inside the fixing sleeve (23), and a through port is formed in the outer side of the special-shaped test tube (25); a fool-proof ring (24) is fixedly connected to the connection part of the fixing sleeve (23) and the housing (1), a notch is formed in the top end of the fool-proof ring (24), a docking plug is fixedly connected to the end of the special-shaped test tube (25), a one-way piece (26) is arranged at the through port of the special-shaped test tube (25), the one-way piece (26) is located inside the special-shaped test tube (25), the one-way piece (26) is made of an elastic material, and one long side of the one-way piece (26) is fixedly connected to the inner wall of the special-shaped test tube (25).
2. The enzyme immunoassay analyzer according to claim 1, characterized in that: a first flip cover (2) is rotatably connected to one side of the housing (1), a recycling drawer (7) is slidably connected to the inside of the housing (1), and the recycling drawer (7) is located at the rear end inside the housing (1).
3. The enzyme immunoassay analyzer according to claim 2, characterized in that: The middle part of the movable disk (14) is arranged in the air. A rotating disk (21) is arranged in the middle part of the movable disk (14). One end of the rotating disk (21) is rotatably connected to the movable disk (14), and the other end of the rotating disk (21) is fixedly connected with a servo motor. The output end of the servo motor is fixedly connected to the movable disk (14). A water absorption pad (16) is arranged below the movable disk (14).
4. An enzyme immunoassay analyzer according to claim 3, characterized in that: Two insertion grooves are formed in the inner wall of the movable disk (14). Spring rods (19) are fixedly connected to both ends of the reagent kit (11). The spring rod (19) is composed of a telescopic rod and a spring.
5. An enzyme immunoassay analyzer according to claim 4, characterized in that: Two rotating wheels (18) are rotatably connected to the inner side of the housing (1). The two rotating wheels (18) are arranged in parallel. The two ends of the water absorption pad (16) are respectively rotatably connected to the ends of the two rotating wheels (18). A plurality of friction pads (20) are fixedly connected to the outer side of the spring rod (19). A transmission belt (17) is sleeved between the two rotating wheels (18). Two extension frames (29) are also rotatably connected to the inner side of the housing (1). The two extension frames (29) are in active contact with the bottom surface of the water absorption pad (16).
6. An enzyme immunoassay analyzer according to claim 5, characterized in that: A sealing pad (10) is arranged between the detection module (9) and the reagent kit (11). A connecting frame (28) is arranged on the inner side of the housing (1). A lifting module is fixedly connected between the connecting frame (28) and the housing (1). The connecting frame (28) includes four cross bars. The sealing pad (10) is fixedly connected to the outer sides of two of the cross bars. A driving motor is fixedly connected to the outer side of one of the cross bars. An extension rod is fixedly connected between the driving motor and the housing (1). Two suction fans are fixedly connected to the bottom surface of the rotating disk (21). Two air suction holes (22) are formed in the top surface of the rotating disk (21). The air suction holes (22) are communicated with the suction fans. An adsorption slit (27) penetrating through to the bottom surface is formed in the top surface of the reagent kit (11) near the edge.
Citation Information
Patent Citations
Semi-automatic immunofluorescence analyzer
CN111487402A