A processing technology for low nucleic acid adsorption centrifuge tube

Abstract

The present invention discloses a processing technology for a centrifuge tube with low nucleic acid adsorption, aiming to provide a centrifuge tube processing technology that is processed separately and then shaped in a mold, and has a good low nucleic acid adsorption effect. The key points of the technical solution are to use a prepared PP material and add a masterbatch with a low adsorption function to the PP material in a molten state, and use the masterbatch with a low adsorption function as a molding element of the low adsorption function. After being melted at a high temperature and mixed evenly, the uniformity is higher. The molding surface of the mold is further mirror-finished, thereby increasing the surface flatness of the centrifuge tube when it is molded in the mold, thereby improving the low adsorption effect. The present invention is applicable to the field of medical equipment technology.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and more specifically, to a processing technology for a nucleic acid low-adsorption centrifuge tube. Background Art

[0002] Nowadays, centrifuge tubes are widely used, especially in life sciences, such as biochemistry, molecular biology, cell biology, microbiology, etc.

[0003] In education and scientific experiments, centrifuge tubes are widely used for sample preservation, chemical reactions, biological extraction and separation, strain preservation, cell and microbial culture, genetic material amplification, etc.

[0004] In hospitals, centrifuge tubes are also widely used for the preservation and analysis of biological and chemical samples.

[0005] Traditionally, low nucleic acid adsorption centrifuge tubes are prepared by surface treating the formed centrifuge tubes. However, due to the shape limitations of the centrifuge tubes themselves, the degree of surface treatment may vary, which may affect the low nucleic acid adsorption effect of the centrifuge tubes. Summary of the Invention

[0006] In view of the shortcomings of the existing technology, the purpose of the present invention is to provide a centrifuge tube processing technology with separate processing, subsequent shaping in a mold and good low nucleic acid adsorption effect.

[0007] To achieve the above-mentioned object, the present invention provides the following technical solution: A process for producing a low-adsorption centrifuge tube for nucleic acid, comprising the following steps: S1, preparing PP material, adding a masterbatch with a low-adsorption function to the molten PP material, melting at high temperature, and mixing uniformly;

[0008] S2. Mirror-finish the molding surface of the mold, configure the punch structure to have multiple outer expansion plates, and install a driving cylinder inside the punch structure for driving the reciprocating motion of each outer expansion plate;

[0009] S3, weaving a first glass fiber and a second glass fiber together to form a barrier layer structure, impregnating the barrier layer with a low-adsorption treatment solution and then drying the mixture, and mixing a portion of the melted PP material with the barrier layer structure to form a combined barrier layer;

[0010] S4. Pre-treating the surface of the combined barrier layer by first cleaning the centrifuge tube to remove oil and organic impurities, and then performing a hydroxylation treatment on the surface;

[0011] S5, treating the surface of the combined barrier layer with a coating, dissolving the modified silane coupling agent in methanol, coating the surface of the combined barrier layer that has been subjected to the hydroxylation treatment, drying it, and then heat-treating it to obtain the coated combined barrier layer;

[0012] S6. The specific operation of the hydroxylation treatment in the surface pretreatment of the combined barrier layer is to prepare a 2.5-3 wt% benzophenone acetone solution, add the combined barrier layer, immerse for 20-25 minutes, take out and irradiate under ultraviolet light for 35-60 seconds;

[0013] S7. Use a robot to put the combined barrier layer on the male mold structure, with the mesh structure side of the barrier layer facing the male mold, and close the upper and lower molds;

[0014] S8, then injecting the mixed molten PP material into the mold and waiting for molding;

[0015] S9. Gradually cool the test mold and press the barrier layer structure into the PP material by controlling the driving cylinder, and the driving cylinder drives the outer expansion sheet to a distance between 0.1mm and 0.3mm;

[0016] S10, further cooling the mold to obtain a finished centrifuge tube.

[0017] The present invention is further configured as follows: the low adsorption treatment solution is prepared by mixing the following components in parts by weight: water, 60-65 parts; aqueous inorganic resin, 35-40 parts; polyethylene glycol, 20-25 parts; sodium lauryl sulfate, 10-12 parts; nanographite, 8-10 parts; and nano titanium dioxide, 6-8 parts.

[0018] The present invention is further configured as follows: the immersion speed of the combined layer structure in the chemical low adsorption treatment solution is 0.5-1m / min; the drying temperature after immersion is 220-260°C, and the time is 30-50 minutes; the silane coupling agent is trimethoxysilane coupling agent, methyltrimethoxysilane coupling agent or methyltriethoxysilane.

[0019] The present invention is further configured such that the ratio between the outer diameter of the outer expansion piece in the expanded state and the outer diameter of the outer expansion piece in the original state is between 1.1 and 1.3.

[0020] The present invention is further configured such that: the mesh density of the mesh structure of the barrier layer is between 1000 mesh and 1200 mesh, and the ratio between the thickness of the mesh structure layer and the thickness of the PP material layer of the barrier layer is between 0.8 and 1.

[0021] The present invention is further configured as follows: a specific method for combining the combined barrier layer and the molten PP material in the mold is as follows: S70, after the combined barrier layer is put on the male mold structure by a robot, the male mold is heated;

[0022] S71, monitoring the melting state of the combined barrier layer in real time, and heating the combined barrier layer until the temperature is between 50° C. and 80° C.;

[0023] S72, injecting the molten PP material into the molding cavity of the mold;

[0024] S73, pressurize the molding cavity after the material is injected, control the pressure in the molding cavity between 30kPa-35kPa, and control the time of the filling stage between 38s-45s.

[0025] S74, maintaining the pressure in the molding cavity, wherein the duration of the pressure holding stage is controlled between 320s and 400s, and the pressure in the cavity is controlled between 30kPa and 35kPa during the pressure holding stage;

[0026] S75, the time of the pressure relief stage is controlled within 10-20s, and the time of the cooling stage is controlled between 250s-300s;

[0027] S76, completing the combination step.

[0028] By adopting the above technical solution, the beneficial effects are as follows: 1. By using a preparatory PP material and adding a masterbatch with a low adsorption function to the PP material in a molten state, the masterbatch with a low adsorption function is used as a molding element of the low adsorption function. After being melted at a high temperature and mixed evenly, the uniformity is higher. The molding surface of the mold is further mirror-finished, which increases the surface flatness of the centrifuge tube when it is molded in the mold, thereby improving the low adsorption effect;

[0029] 2. The barrier layer structure is formed by weaving a mixture of the first glass fiber and the second glass fiber, and then impregnated with a low-adsorption treatment solution and then dried. Part of the molten PP material is mixed with the barrier layer structure to form a combined barrier layer. With the above-mentioned structural arrangement, the barrier layer structure serves as a structural carrier of the PP material. After the barrier layer is formed, a separate low-adsorption surface treatment is performed on the barrier layer, thereby increasing the comprehensiveness of the low-adsorption surface treatment.

[0030] 3. The barrier layer after low-adsorption surface treatment is re-molded with the PP material in the mold to improve the low-adsorption capacity of the centrifuge tube after molding. It is highly practical. The split treatment and then integration through the mold achieve the low-adsorption effect of the centrifuge tube as a whole, and the low-adsorption area covers a wide range.

[0031] 4. In the processing technology of the present invention, in order to ensure that the combined barrier layer and the PP material in the molten state can be well integrated in the mold, the convex mold is heated to achieve a good bonding effect, and then the pressure is maintained after pressurization to ensure that the combined barrier layer can be fully integrated and has strong stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The present invention is a flowchart of a processing method for a low-adsorption centrifuge tube for nucleic acids. DETAILED DESCRIPTION

[0033] Reference Figure 1 The processing technology embodiment of a low nucleic acid adsorption centrifuge tube of the present invention is further described.

[0034] For ease of explanation, spatial relative terms such as "upper", "lower", "left", and "right" are used in the embodiments to illustrate the relationship between one element or feature shown in the figures and another element or feature. It should be understood that, in addition to the orientation shown in the figures, spatial terms are intended to include different orientations of the device in use or operation. For example, if the device in the figure is inverted, the element described as being "under" other elements or features will be positioned "above" other elements or features. Therefore, the exemplary term "under" can include both upper and lower orientations. The device can be positioned in other ways (rotated 90 degrees or in other orientations), and the spatial relative descriptions used here can be interpreted accordingly.

[0035] Moreover, relational terms such as “first” and “second” are merely used to distinguish one component from another having the same name, but do not necessarily require or imply any actual relationship or order between these components.

[0036] A process for producing a nucleic acid low-adsorption centrifuge tube comprises the following steps: S1, preparing PP material, adding a masterbatch with a low-adsorption function to the molten PP material, melting the material at high temperature, and mixing the material uniformly;

[0037] S2. Mirror-finish the molding surface of the mold, configure the punch structure to have multiple outer expansion plates, and install a driving cylinder inside the punch structure for driving the reciprocating motion of each outer expansion plate;

[0038] By using a preparatory PP material and adding a masterbatch with a low adsorption function into the molten PP material, the masterbatch with a low adsorption function is used as a molding element of the low adsorption function. After being melted at a high temperature and mixed evenly, the uniformity is higher. The molding surface of the mold is further mirror-finished, which increases the surface flatness of the centrifuge tube when it is molded in the mold, thereby improving the low adsorption effect.

[0039] S3, weaving a first glass fiber and a second glass fiber together to form a barrier layer structure, impregnating the barrier layer with a low-adsorption treatment solution and then drying the mixture, and mixing a portion of the melted PP material with the barrier layer structure to form a combined barrier layer;

[0040] The barrier layer structure is formed by weaving a mixture of a first glass fiber and a second glass fiber, and then impregnating the mixture with a low-adsorption treatment solution and drying the mixture. A partially molten PP material is mixed with the barrier layer structure to form a combined barrier layer. With the above-mentioned structural arrangement, the barrier layer structure serves as a structural carrier of the PP material. After the barrier layer is formed, a separate low-adsorption surface treatment is performed on the barrier layer, thereby increasing the comprehensiveness of the low-adsorption surface treatment.

[0041] S4. Pre-treating the surface of the combined barrier layer by first cleaning the centrifuge tube to remove oil and organic impurities, and then performing a hydroxylation treatment on the surface;

[0042] S5, treating the surface of the combined barrier layer with a coating, dissolving the modified silane coupling agent in methanol, coating the surface of the combined barrier layer that has been subjected to the hydroxylation treatment, drying it, and then heat-treating it to obtain the coated combined barrier layer;

[0043] S6. The specific operation of the hydroxylation treatment in the surface pretreatment of the combined barrier layer is to prepare a 2.5-3 wt% benzophenone acetone solution, add the combined barrier layer, immerse for 20-25 minutes, take out and irradiate under ultraviolet light for 35-60 seconds;

[0044] S7. Use a robot to put the combined barrier layer on the male mold structure, with the mesh structure side of the barrier layer facing the male mold, and close the upper and lower molds;

[0045] S8. The mixed molten PP material is then injected into a mold for molding, and the barrier layer that has undergone low-adsorption surface treatment is molded again with the PP material in the mold to improve the low-adsorption capacity of the centrifuge tube after molding. This method is highly practical. The split treatment is then integrated through the mold to achieve a low-adsorption effect for the entire centrifuge tube, and the low-adsorption area covers a wide range.

[0046] S9. The test mold is gradually cooled, and the barrier layer structure is pressed into the PP material by controlling the driving cylinder, and the distance of the driving cylinder driving the outer expansion sheet is between 0.1mm and 0.3mm. The above steps are performed to achieve a full combination of the barrier layer structure and the PP material in the mold, thereby improving the good bonding effect between the two and having strong practicality.

[0047] S10, further cooling the mold to obtain a finished centrifuge tube.

[0048] The present invention is further configured such that the low adsorption treatment solution is prepared by mixing the following components in parts by weight: water, 60-65 parts; aqueous inorganic resin, 35-40 parts; polyethylene glycol, 20-25 parts; sodium lauryl sulfate, 10-12 parts; nanographite, 8-10 parts; and nano titanium dioxide, 6-8 parts.

[0049] The present invention is further configured such that the immersion speed of the combined layer structure in the chemical low adsorption treatment solution is 0.5-1 m / min; the drying temperature after immersion is 220-260°C, and the time is 30-50 minutes; the silane coupling agent is trimethoxysilane coupling agent, methyltrimethoxysilane coupling agent or methyltriethoxysilane.

[0050] By adopting the above-mentioned structural setting, the mesh structure of the barrier layer is subjected to low-adsorption treatment, thereby achieving an overall low-adsorption effect. Moreover, the mesh density of the mesh structure of the barrier layer is between 1000 mesh and 1200 mesh. By controlling the mesh density of the mesh structure of the barrier layer, the integrity between the mesh structure and the PP material is ensured, thereby increasing the overall low-absorption effect. Moreover, the ratio between the thickness of the mesh structure layer and the thickness of the PP material layer of the barrier layer is between 0.8 and 1, thereby ensuring good integrity in the mold and strong practicality.

[0051] The present invention is further configured such that the ratio between the outer diameter of the outer expansion piece in the expanded state and the outer diameter of the outer expansion piece in the original state is between 1.1 and 1.3.

[0052] The present invention is further configured such that a specific method for combining the combined barrier layer and the molten PP material in the mold is as follows: S70, after the combined barrier layer is put on the male mold structure by a robot, the male mold is heated;

[0053] S71, monitoring the melting state of the combined barrier layer in real time, and heating the combined barrier layer until the temperature is between 50° C. and 80° C.;

[0054] S72, injecting the molten PP material into the molding cavity of the mold;

[0055] S73, pressurize the molding cavity after the material is injected, control the pressure in the molding cavity between 30kPa-35kPa, and control the time of the filling stage between 38s-45s.

[0056] S74, maintaining the pressure in the molding cavity, wherein the duration of the pressure holding stage is controlled between 320s and 400s, and the pressure in the cavity is controlled between 30kPa and 35kPa during the pressure holding stage;

[0057] S75, the time of the pressure relief stage is controlled within 10-20s, and the time of the cooling stage is controlled between 250s-300s;

[0058] S76, completing the combination step.

[0059] In the processing technology of the present invention, in order to ensure that the combined barrier layer and the PP material in the molten state can be well fused in the mold, the punch is heated to achieve a good bonding effect, and then the pressure is maintained after filling and pressing to ensure that the combined barrier layer can be fully bonded and has strong stability.

[0060] In an embodiment of the present invention, a method for preparing a water-based inorganic resin includes: step S1, preparing raw materials by weight: potassium silicate, 60 parts; silica sol, 20 parts; lithium silicate, 5 parts; silane coupling agent, 4 parts; styrene acrylic emulsion, 10 parts; magnesium chloride, 2 parts; disodium ethylenediaminetetraacetic acid, which is 4 times the weight of magnesium chloride; deionized water, 20 parts; the potassium silicate modulus is 3.3-3.5, and the silicon dioxide content is 19-21wt%; the silicon dioxide content in the silica sol is 25-26wt%, and the sodium oxide content is 0.2-0.4wt%; the lithium silicate modulus is 4.7-4.9, and the silicon dioxide content is 19-21wt%; the solid content of the styrene acrylic emulsion is 46-48wt%;

[0061] Step S2: first add deionized water into the dispersion kettle, adjust the speed to 700 r / min, then add potassium silicate, silica sol and lithium silicate, and stir continuously for 5 minutes;

[0062] Step S3: Finally, the silane coupling agent was added dropwise within 20 minutes, the rotation speed was adjusted to 500 r / min, and the mixture was stirred continuously for 1.5 hours, then allowed to stand for 30 hours, and the remaining raw materials were added, stirred continuously for 50 minutes, and filtered to obtain the product.

[0063] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Common changes and substitutions made by those skilled in the art within the scope of the technical solution of the present invention should be included in the protection scope of the present invention.

Claims

1. A processing technology for a nucleic acid low adsorption centrifuge tube, characterized in that: The following steps are included: S1. Prepare materials: Prepare PP material, add masterbatch with low adsorption function into the molten PP material, melt at high temperature and mix evenly; S2. Mirror treatment of the mold: Mirror treatment is performed on the molding surface of the mold, and the punch structure is set as a multi-outer expansion plate structure, and a driving cylinder for driving the reciprocating motion of each outer expansion plate is installed in the punch structure; S3, barrier layer structure formation: the first glass fiber and the second glass fiber are mixed and woven to form a barrier layer structure, which is then impregnated with a low-adsorption treatment solution and then dried. Part of the molten PP material is mixed with the barrier layer structure to form a combined barrier layer; S4. Preliminary surface treatment of the combined barrier layer: performing surface pretreatment on the combined barrier layer, first cleaning the surface of the combined barrier layer to remove oil and organic impurities, and then performing hydroxylation treatment on the surface; S5. Surface hydroxylation treatment of the combined barrier layer: Specifically, prepare a 2.5-3 wt% benzophenone acetone solution, add the combined barrier layer, immerse for 20-25 minutes, take out and irradiate under ultraviolet light for 35 seconds to 60 seconds; S6. Surface coating treatment of the combined barrier layer: dissolving the modified silane coupling agent in methanol, coating the surface of the combined barrier layer after the hydroxylation treatment, drying it, and then heat-treating it to obtain the coated combined barrier layer; S7. Loading the combined barrier layer: The combined barrier layer is placed on the punch structure by a robot, with the mesh structure side of the barrier layer facing the punch, and the upper and lower molds are closed; The specific combination method between the combined barrier layer and the molten PP material in the mold is as follows: S70, after the combined barrier layer is put on the male mold structure by a robot, the male mold is heated; S71, monitoring the melting state of the combined barrier layer in real time, and heating the combined barrier layer until the temperature is between 50° C. and 80° C.; S72, injecting the molten PP material into the molding cavity of the mold; S73, pressurizing the molding cavity after the injected material, controlling the pressure in the molding cavity to be between 30kPa-35kPa, and controlling the time of the filling stage to be between 38s-45s; S74, maintaining the pressure in the molding cavity, wherein the duration of the pressure holding stage is controlled between 320s and 400s, and the pressure in the cavity is controlled between 30kPa and 35kPa during the pressure holding stage; S75, the time of the pressure relief stage is controlled within 10-20s, and the time of the cooling stage is controlled between 250s-300s; S76, completing the combination step; S8, injection molding: inject the mixed molten PP material into the mold and wait for molding; S9, cooling extrusion: gradually cool the test mold, and press the barrier layer structure into the PP material by controlling the driving cylinder, and the driving cylinder drives the outer expansion sheet to a distance between 0.1mm and 0.3mm; S10, cooling completed: the mold is further cooled to obtain a finished centrifuge tube.

2. The processing technology of a nucleic acid low adsorption centrifuge tube according to claim 1, characterized in that: The low adsorption treatment solution is prepared by mixing the following components in parts by weight: water, 60-65 parts; aqueous inorganic resin, 35-40 parts; polyethylene glycol, 20-25 parts; sodium lauryl sulfate, 10-12 parts; nanographite, 8-10 parts; nano titanium dioxide, 6-8 parts, The preparation method of the water-based inorganic resin comprises: step S1, preparing raw materials by weight: potassium silicate, 60 parts; silica sol, 20 parts; lithium silicate, 5 parts; silicon coupling agent, 4 parts; styrene acrylic emulsion, 10 parts; magnesium chloride. 2 parts: disodium ethylenediaminetetraacetic acid, 4 times the weight of magnesium chloride; deionized water, 20 parts; the potassium silicate modulus is 3.3-3.5, the silica content is 19-21wt%; the silica content in the silica sol is 25-26w%, and the sodium oxide content is 0.2-0.4wt%; the lithium silicate modulus is 4.7-4.9, the silica content is 19-21wt%; the styrene acrylic emulsion solid content is 46-48wt%; Step S2, first deionized water was added to the dispersion vessel, the speed was adjusted to 700r / min, and then potassium silicate, silica sol and lithium silicate were added, and stirring was continued for 5 minutes; Step S3: Finally, the silane coupling agent was added dropwise within 20 minutes, the rotation speed was adjusted to 500 r / min, and the mixture was stirred continuously for 1.5 hours, then allowed to stand for 30 hours, and the remaining raw materials were added, stirred continuously for 50 minutes, and filtered to obtain the product.

3. The processing technology of a nucleic acid low adsorption centrifuge tube according to claim 2, characterized in that: The barrier layer structure is immersed in the chemical low adsorption treatment solution at a speed of 0.5-1 m / min; the drying temperature after immersion is 220-260° C., and the drying time is 30-50 minutes; the silane coupling agent is trimethoxysilane coupling agent, methyltrimethoxysilane coupling agent or methyltriethoxysilane.

4. The processing technology of a nucleic acid low adsorption centrifuge tube according to claim 1, characterized in that: The ratio between the outer diameter of the outer expansion piece in the expanded state and the outer diameter of the outer expansion piece in the original state is between 1.1 and 1.

3.

5. The processing technology of a nucleic acid low adsorption centrifuge tube according to claim 1, characterized in that: The mesh density of the network structure of the barrier layer is between 1000 mesh and 1200 mesh, and the ratio between the thickness of the network structure layer and the thickness of the PP material layer of the barrier layer is between 0.8 and 1.

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