Magnetic nanomedia magneto-thermo-acoustic multi-field coupling neuromodulation method and device

Abstract

The application provides a kind of magnetic nanometer medium magnetoacoustic multi-field coupling nerve regulation method and device, magnetic field is generated by coil, magnetic nanometer medium located in brain function area is excited, magnetic field excitation acts on magnetic nanometer medium, causes thermal expansion, magnetoacoustic or magnetic force, through the potential change of ultrasound, magnetic force and further generation caused by magnetoacoustic and electromagnetic excitation, the regulation of neuron is realized;The magnetic, thermal, force, acoustic, electric coupling physical effects generated by electromagnetic nanometer medium of biological target are used, membrane depolarization is triggered, the biological effects of intracellular calcium ion inflow of neuron are induced, the intracellular calcium signal conduction is regulated to affect synaptic plasticity and control neural behavior, and then precise controllable regulation of excitability of brain area neuron subgroup is realized.The magnetic nanometer medium magnetoacoustic multi-field coupling nerve regulation device using the method of the application is composed of multi-mode electromagnetic excitation unit, coil unit with focusable electromagnetic field distribution, coupling conversion unit and test unit.

Description

Magnetic Nanomaterial Magnetothermal-Acoustic Multi-Field Coupled Neuromodulation Method and Device Technical Field

[0001] This invention belongs to the field of neuromodulation technology, specifically relating to a method and device for multi-field coupling of magnetic nanomaterials with magnetothermal acoustics. Background Technology

[0002] With the rise and development of neuroscience and cognitive science, research on human-centered cognitive and intelligent activities has entered a new stage of development. The nervous system controls overall human health and physical capabilities; enhancing the efficiency of the nervous system can improve learning efficiency, reduce fatigue, and enhance alertness, perception, and decision-making abilities. Due to global population growth and the increasing severity of aging, neurological diseases have become one of the leading causes of disability and death worldwide. Regulating emotions, motivation, and cognitive recognition through neuromodulation is an important direction in neuromodulation technology. How to conduct in-depth and precise regulation of the nervous system has immense theoretical value and practical significance for neuroenhancement and treatment.

[0003] Traditional deep brain stimulation (DBS) is achieved through the implantation of permanent electrodes, a invasive technique. Second-generation neuromodulation techniques, relying on stimulation methods such as sound and electromagnetic induction, offer new pathways for nervous system regulation, yielding a series of research results and showing greater promise for clinical applications. Currently, commonly used non-invasive brain stimulation methods include transcranial direct current stimulation (TCD), transcranial magnetic stimulation (TMS), and transcranial focused ultrasound (TUS).

[0004] The above methods represent significant breakthroughs in the field of non-invasive brain stimulation. However, due to tissue absorption and dispersion, limitations remain in spatial resolution, penetration depth, and targeting ability. Therefore, it is of great significance to achieve specific targeting of deep brain stimulation and to solve the problems of low precision and insufficient neural activation efficiency in neuroelectromagnetic modulation stimulation.

[0005] With the development and application of various types of targeted nanoparticle formulations, the biological application research of nanoparticle materials has become a cutting-edge hot topic in the fields of nanobiology and nanomedicine. With the interdisciplinary integration of brain science and nanotechnology, the application of neuroactive nanoparticles in brain neuromodulation research has proven to have significant practical value in areas such as enhancing human function and regulating neurological diseases. Currently reported nanoparticle neuromodulation technologies are all techniques that stimulate nanoparticles in a single field to produce biological effects, and they still have significant shortcomings in terms of stimulation efficiency and precise regulation. This invention proposes a magnetic nanomedia magnetothermal-acoustic multi-field coupling neuromodulation method. Through the coupling physical effects of electromagnetic, thermal, mechanical, and ultrasonic forces, this method is not merely a simple superposition of multiple physical effects. The magnetic nanomedia magnetothermal-acoustic multi-field coupling neuromodulation method is a non-invasive, targeted neurostimulation technique. By applying external stimulation, it utilizes the magnetic, thermal, mechanical, and acoustic physical effects generated by electromagnetic nanoparticles at biological targets to induce membrane depolarization, induce calcium ion influx in neurons, and regulate intracellular calcium signal transduction, affecting synaptic plasticity and controlling neural behavior, thereby achieving precise and controllable regulation of the excitability of neuronal subsets in brain regions. It can regulate emotions, cognition, thinking and reasoning abilities, promote the extraordinary performance of physical, intellectual and psychological qualities, and achieve the effects of nerve enhancement and treatment, and enhancement of individual functions and abilities.

[0006] By utilizing the multi-field coupling physical effect and the further biological effect induced by magnetic nanomaterials under electromagnetic excitation, high-precision targeted modulation of neurons can be achieved. This can overcome the shortcomings of existing single-physical-field non-invasive neurostimulation in terms of spatial resolution, stimulation penetration depth, and stimulation precision, and provide a new approach for minimally invasive, non-contact, and precise electromagnetic modulation of brain nerves. It has great application prospects in disease treatment and brain functional network research. Summary of the Invention

[0007] To address the aforementioned technical problems, this invention provides a magnetic nanomaterial magnetothermal-acoustic multi-field coupling neuromodulation method and device to achieve high-precision targeted regulation of neurons, realize specific targeting of deep brain stimulation, and solve the problems of low precision and insufficient nerve activation efficiency in neuroelectromagnetic modulation stimulation. It can compensate for the shortcomings of existing non-invasive neurostimulation in terms of spatial resolution, stimulation penetration depth, and stimulation precision. This invention proposes a magnetic nanomaterial magnetothermal-acoustic multi-field coupling neuromodulation method and device, providing a new approach to minimally invasive, non-contact, and highly effective precise electromagnetic modulation of brain nerves.

[0008] This invention utilizes electromagnetic excitation of magnetic nanomaterials to generate thermal, mechanical, ultrasonic, and electrical potential signals. These signals are then applied to biological targets through a multi-physics field coupling effect involving electromagnetics, temperature, force, and sound, producing biological effects and enabling precise and controllable regulation of the excitability of neuronal subpopulations in brain regions. By employing efficient and biosafe magnetic nanomaterials, multi-physics field focusing, and highly efficient electromagnetic field excitation technology and equipment, this invention achieves non-invasive, high-precision control of brain nerve function and corresponding neural behavior, providing a new approach to non-contact, precise brain nerve regulation.

[0009] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0010] A magnetic nanomaterial magnetothermal-acoustic multi-field coupling neural modulation method is proposed. This method utilizes an external excitation coil to generate a pulsed magnetic field, which excites the magnetic nanomaterial located at a biological target site in a brain functional area. The magnetic field excitation acts on the magnetic nanomaterial, inducing thermal expansion, magnetothermal-acoustic or magnetic force. Through the ultrasound, magnetic force, and further potential changes caused by magnetothermal, magnetothermal-acoustic, and electromagnetic excitation, neuronal modulation is achieved. Furthermore, the method utilizes the magnetic, thermal, mechanical, acoustic, and electrical coupling physical effects generated by the electromagnetic nanomaterial at the biological target site to induce membrane depolarization and induce calcium ion influx in neurons, thereby regulating intracellular calcium signal transduction, influencing synaptic plasticity, and controlling neural behavior. Ultimately, this achieves precise and controllable regulation of the excitability of neuronal subsets in brain regions.

[0011] Furthermore, the magnetic nanomediator is a magnetic nanoparticle or a magnetic nanoparticle molecular probe prepared from magnetic nanoparticles. The magnetic nanoparticle molecular probe is composed of magnetic nanoparticles, a carrier, a ligand, or an antibody. The magnetic nanoparticles include Fe3O4, cobalt-iron-manganese-iron, spherical, and polyhedral magnetic nanoparticles, which are used to enhance the physical effect of electromagnetic field excitation of the target region. The ligand or antibody is used to bind to the receptor highly expressed in the target region. The electromagnetic and acoustic response characteristics of the magnetic nanoparticles, the thickness and concentration characteristics of the external modification all affect the physical effect produced by electromagnetic excitation of the magnetic nanomediator.

[0012] Furthermore, by exciting the magnetic nanomedium with an electromagnetic field, the physical effects of the magnetic nanoparticles under electromagnetic field excitation, including magnetocaloric, magnetocaloric-acoustic, and magnetic effects, as well as the induced potential, are utilized to generate biological effects, namely, activating the thermosensitive capsaicin receptor TRPV1 in neurons and inducing the release of calcium ions (Ca) in neurons. 2+Influx of ions leads to an increase in intracellular cation concentration, causing corresponding physiological changes. This study analyzes the effects of the coupled physical effects of different concentrations of magnetic nanoparticles under external excitation on ion channel gating characteristics and synaptic plasticity, establishing the relationship between physical and biological effects. Through calcium indicators and membrane potential observation, the flow of calcium ions inside and outside the cell is controlled, achieving intracellular ion control. By establishing the relationship between physical and biological effects, and combining it with electromagnetic excitation characteristics, the study establishes the relationship between the biological effects induced by magnetic nanoparticles under external excitation and neural regulation. Furthermore, it establishes the relationship between exogenous excitation characteristics, magnetic nanoparticle characteristic parameters, and neural regulation, establishing a spatiotemporal dose-response relationship for activating neural function, thus forming precise neural regulation.

[0013] This invention also provides a magnetic nanomaterial magnetocaloric multi-field coupling neural modulation device for implementing a magnetic nanomaterial magnetocaloric multi-field coupling neural modulation method. The device includes a multi-mode electromagnetic excitation unit, a coil unit capable of focusing electromagnetic field distribution, a coupling conversion unit, and a testing unit. The multi-mode electromagnetic excitation unit generates alternating excitation, modulated signal alternating pulse excitation, and single-pulse excitation with a certain repetition frequency. This unit provides high-power excitation to the coil load. The coupling conversion unit converts electromagnetic excitation to magnetocaloric, magnetocaloric, and magnetic forces. The testing unit tests the multi-mode electromagnetic excitation unit, the electromagnetic field distribution, the physical effects generated by the electromagnetic excitation coupling conversion, and the... The biological effect generated in one step can focus the electromagnetic field distribution of the coil unit as the load of the multi-mode electromagnetic excitation unit and generate electromagnetic fields with different distributions; the output of the multi-mode electromagnetic excitation unit is connected to the input of the coupling conversion unit, and the output of the coupling conversion unit is connected to the input of the test unit; the multi-mode electromagnetic excitation unit outputs a current excitation signal to the coupling conversion unit, the coupling conversion unit outputs a signal to the test unit, and the multi-mode electromagnetic excitation unit simultaneously outputs a current signal to the input of the test unit. The test unit outputs a signal to the input of the multi-mode electromagnetic excitation unit according to the tested current parameters to adjust the drive signal of the multi-mode electromagnetic excitation unit.

[0014] Furthermore, the multi-mode electromagnetic excitation unit consists of a drive signal generator, a power amplifier unit, and an excitation coil matching module; the output terminal of the drive signal generator is connected to the input terminal of the power amplifier unit, and the output terminal of the power amplifier unit is connected to the input terminal of the excitation coil matching module; the drive signal generator outputs a signal to the power amplifier unit, the power amplifier unit outputs a signal to the excitation coil matching module, and the excitation current is applied to the excitation coil through the excitation coil matching module.

[0015] Furthermore, the structure of the coil unit capable of focusing electromagnetic field distribution is a solenoid coil, a planar coil, an H-type coil, or an array coil. The electromagnetic field distribution characteristics are adjusted according to different coil structures, number of turns, diameter, and wire diameter parameters to generate the required focused electromagnetic field distribution.

[0016] Furthermore, the coupling conversion unit consists of an excitation coil, a magnetic nanomedium, a calcium indicator, and a head-controlled target body. The magnetic nanomedium and the calcium indicator are located in the head-controlled target area. The excitation coil is close to the target area. When the multi-mode electromagnetic excitation unit excites the electromagnetic field through the excitation coil, it generates magnetothermal, magnetothermal acoustic, and magnetic force signals, thereby stimulating biological effects, inducing calcium ion influx, leading to an increase in intracellular cation concentration, and the calcium indicator shows a change in fluorescence intensity.

[0017] Furthermore, the testing unit includes an excitation source operating status parameter acquisition module, an excitation current detection module, an electromagnetic field tester, a temperature detection module, a magnetothermal-acoustic signal detection module, a micro-magnetic force detection module, a fluorescence imaging system, and a water cooling system. The excitation source operating status parameter acquisition module outputs a signal to the input terminal of the multi-mode electromagnetic excitation unit based on the current parameters tested by the excitation current detection module, adjusting the drive signal of the multi-mode electromagnetic excitation unit. The excitation current detection module is used to detect the current signal generated by the multi-mode electromagnetic excitation unit through the excitation coil. The electromagnetic field tester tests the electromagnetic field distribution characteristics generated near the excitation coil. The temperature detection module is used to detect the temperature of the head and the magnetic nanomaterial. The magnetothermal-acoustic signal detection module is used to detect the magnetothermal-acoustic signal generated by the excitation coil exciting the magnetic nanomaterial. The micro-magnetic force detection module is used to detect the magnetic force signal generated by the excitation coil exciting the magnetic nanomaterial. The fluorescence imaging system is used to observe the change in fluorescence intensity caused by calcium ion influx. The water cooling system is used to cool the multi-mode electromagnetic excitation unit and the coil unit.

[0018] Beneficial effects:

[0019] The magnetic nanomaterial magnetocaloric multi-field coupled neural modulation method and device of the present invention does not utilize the acoustic waves excited by the instantaneous thermal expansion generated by electromagnetic excitation to invert the electrical parameters of the medium; that is, it does not use the conversion of instantaneous thermal expansion to acoustic signals for inversion while ignoring thermal conduction. The magnetocaloric, magnetocaloric acoustic, and magnetic physical effects excited by the present invention act directly on the biological target area. That is, it utilizes the magnetocaloric and thermal conduction properties to directly induce biological effects in the target area. Its principle differs from the magnetocaloric acoustic imaging method. Due to the different principles and the different physical effects produced, the excitation modes and characteristics used are different to produce better biological effects: alternating excitation with a high repetition frequency (400kHz~550kHz) and alternating pulse excitation of modulation signals are more conducive to the control of the magnetocaloric effect; single pulse excitation with a certain repetition frequency is more conducive to the control of the magnetic effect; all of the above excitation methods are conducive to the control of the magnetocaloric acoustic effect.

[0020] The magnetic nanomaterial magnetothermal-acoustic multi-field coupling neural modulation method and device of the present invention utilizes the physical effect of magnetic nanomaterials under electromagnetic excitation to act on biological targets, which can achieve high-precision targeted modulation of neurons. It can make up for the shortcomings of existing single physical field non-invasive neural stimulation in terms of spatial resolution, stimulation penetration depth and stimulation precision, and provide a new idea for minimally invasive, non-contact and precise electromagnetic modulation of brain nerves. It has great application prospects in disease treatment and brain functional network research. Attached Figure Description

[0021] Figure 1 is a schematic diagram of the magnetic nanomaterial magnetothermal acoustic multi-field coupling neural modulation method of the present invention.

[0022] Figure 2 is a schematic diagram of biological effects and precise neural modulation methods;

[0023] Figure 3 is a schematic diagram of an embodiment of a magnetic nanomaterial magnetothermal-acoustic multi-field coupled neural modulation device. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention. Furthermore, the technical features involved in the various embodiments of this invention described below can be combined with each other as long as they do not conflict with each other.

[0025] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0026] This invention utilizes electromagnetic excitation of magnetic nanomaterials to generate thermal, mechanical, ultrasonic, and electrical potential signals. Through the multi-physics coupling effect of electromagnetic-temperature-force-sound, these signals act on biological targets, producing biological effects and enabling precise and controllable regulation of the excitability of neuronal subpopulations in brain regions. By employing efficient and biosafe magnetic nanomaterials, multi-physics focusing, and efficient electromagnetic field excitation technology and equipment, non-invasive and highly precise control of brain nerve function and corresponding neural behavior can be achieved, providing a new approach to non-contact, precise brain nerve regulation.

[0027] As shown in Figure 1, this invention utilizes an external excitation coil to generate a pulsed magnetic field, which excites the magnetic nanomaterials located at biological target sites in brain functional areas. The magnetic field excitation acts on the magnetic nanomaterials, inducing thermal expansion, magnetothermal acoustics, or magnetic force. Through the ultrasound, magnetic force, and further potential changes caused by magnetothermal, magnetothermal acoustics, and electromagnetic excitation, the regulation of neurons is achieved. The magnetic, thermal, mechanical, acoustic, and electrical coupling physical effects generated by the electromagnetic nanomaterials at the biological target sites induce membrane depolarization and induce calcium ion influx in neurons, regulating intracellular calcium signal transduction, thereby affecting synaptic plasticity and controlling neural behavior, and thus achieving precise and controllable regulation of the excitability of neuronal subpopulations in brain regions. Specifically, when the magnetothermal effect causes the temperature T of the target area to reach 43°C, most of the TRPV1 channels open, and the resulting calcium ion influx is sufficient to trigger neuronal activity.

[0028] Figure 2 illustrates the biological effects and precise neuromodulation methods. By exciting magnetic nanomaterials with an electromagnetic field, the physical effects of the magnetic nanoparticles under electromagnetic field excitation—including magnetocaloric, magnetocaloric-acoustic, and magnetic force effects, as well as the induced potential—are utilized to generate biological effects, such as activating the neuronal thermosensitive capsaicin receptor TRPV1 and inducing the release of calcium ions (Ca) into neurons. 2+ Influx of ions leads to an increase in intracellular cation concentration, causing corresponding physiological changes. This study analyzes the effects of the coupled physical effects of different concentrations of magnetic nanoparticles under external excitation on ion channel gating characteristics and synaptic plasticity, establishing the relationship between physical and biological effects. Through calcium indicators and membrane potential observation, the flow of calcium ions inside and outside the cell is controlled, achieving intracellular ion control. By establishing the relationship between physical and biological effects, and combining it with electromagnetic excitation characteristics, the study establishes the relationship between the biological effects induced by magnetic nanoparticles under external excitation and neural regulation. Furthermore, it establishes the relationship between exogenous excitation characteristics, magnetic nanoparticle characteristic parameters, and neural regulation, establishing a spatiotemporal dose-response relationship for activating neural function, thus forming precise neural regulation.

[0029] The performance of magnetic nanomaterials in activating neural function through magnetothermal-acoustic multi-field coupling depends largely on the properties of the magnetic nanomaterials and their coupling efficiency with an external magnetic field. Ultimately, it manifests in the activation efficacy of the magnetic field-induced physical effects of the magnetic nanomaterials on biological effects. Ion channels, as targets of nanomaterials in the action of neurons, participate in the regulation of important physiological processes such as hormone secretion, neurotransmitter release, signal transduction, neuronal electrical activity, synaptic signal transmission, and cell growth.

[0030] Figure 3 shows an embodiment of the magnetic nanomaterial magnetothermal-acoustic multi-field coupled neural modulation device of the present invention. As shown in Figure 3, the modulation device includes a multi-mode electromagnetic excitation unit, a coil unit that can focus electromagnetic field distribution, a coupling conversion unit, and a testing unit.

[0031] The multi-mode electromagnetic excitation unit generates alternating excitation, modulated signal alternating pulse excitation, and single pulse excitation with a certain repetition frequency. The multi-mode electromagnetic excitation unit provides high-power excitation to the coil load. The coupling conversion unit converts electromagnetic excitation to magnetocaloric, magnetocaloric acoustic, and magnetic excitation. The testing unit tests the multi-mode electromagnetic excitation unit, electromagnetic field distribution, the physical effects generated by electromagnetic excitation coupling conversion, and further biological effects. A coil unit capable of focusing electromagnetic field distribution serves as the load for the multi-mode electromagnetic excitation unit and generates electromagnetic fields with different distributions. The output of the multi-mode electromagnetic excitation unit is connected to the input of the coupling conversion unit, and the output of the coupling conversion unit is connected to the input of the testing unit. The multi-mode electromagnetic excitation unit outputs a current excitation signal to the coupling conversion unit, and the coupling conversion unit outputs a signal to the testing unit. Simultaneously, the multi-mode electromagnetic excitation unit outputs a current signal to the input of the testing unit. The testing unit outputs a signal to the input of the multi-mode electromagnetic excitation unit based on the tested current parameters, adjusting the driving signal of the multi-mode electromagnetic excitation unit.

[0032] The multi-mode electromagnetic excitation unit consists of a drive signal generator, a power amplifier unit, and an excitation coil matching module. The output terminal of the drive signal generator is connected to the input terminal of the power amplifier unit, and the output terminal of the power amplifier unit is connected to the input terminal of the excitation coil matching module. The drive signal generator outputs a signal to the power amplifier unit, and the power amplifier unit outputs a signal to the excitation coil matching module. Through the excitation coil matching module, the excitation current is applied to the excitation coil.

[0033] The structure of the coil unit that can focus electromagnetic field distribution is a solenoid coil, a planar coil, an H-type coil, or an array coil. The electromagnetic field distribution characteristics are adjusted according to different coil structures, number of turns, diameter, and wire diameter parameters to generate the required focused electromagnetic field distribution.

[0034] The coupling conversion unit consists of an excitation coil, a magnetic nanomedium, a calcium indicator, and a head-controlled target. The magnetic nanomedium and the calcium indicator are located in the head-controlled target area. The excitation coil is close to the target area. When the multi-mode electromagnetic excitation unit excites the electromagnetic field through the excitation coil, it generates magnetothermal, magnetothermal acoustic, and magnetic force signals, thereby stimulating biological effects, inducing calcium ion influx, leading to an increase in intracellular cation concentration, and the calcium indicator shows a change in fluorescence intensity.

[0035] The testing unit includes an excitation source operating status parameter acquisition module, an excitation current detection module, an electromagnetic field tester, a temperature detection module, a magnetothermal-acoustic signal detection module, a micro-magnetic force detection module, a fluorescence imaging system, and a water cooling system. The excitation source operating status parameter acquisition module outputs a signal to the input of the multi-mode electromagnetic excitation unit based on the current parameters measured by the excitation current detection module, adjusting the drive signal of the multi-mode electromagnetic excitation unit. The excitation current detection module detects the current signal generated by the multi-mode electromagnetic excitation unit through the excitation coil. The electromagnetic field tester measures the electromagnetic field distribution characteristics near the excitation coil. The temperature detection module detects the temperature of the head and the magnetic nanomaterial. The magnetothermal-acoustic signal detection module detects the magnetothermal-acoustic signal generated by the excitation coil exciting the magnetic nanomaterial. The micro-magnetic force detection module detects the magnetic force signal generated by the excitation coil exciting the magnetic nanomaterial. The fluorescence imaging system observes the changes in fluorescence intensity caused by calcium ion influx. The water cooling system cools the multi-mode electromagnetic excitation unit and the coil unit.

[0036] The magnetic nanomedia used in the magnetic nanomedia magnetothermal-acoustic multi-field coupling neural modulation method of the present invention are magnetic nanoparticles or magnetic nanoparticle molecular probes prepared from magnetic nanoparticles. The magnetic nanoparticle molecular probes are composed of magnetic nanoparticles, carriers, ligands or antibodies. The magnetic nanoparticles include Fe3O4, cobalt-iron-manganese-iron, spherical, and polyhedral magnetic nanoparticles, which are used to enhance the physical effect of electromagnetic field excitation of the target region. The ligands or antibodies are used to bind to the receptors highly expressed in the target region. The electromagnetic and acoustic response characteristics of the magnetic nanoparticles, the thickness and concentration characteristics of the external modification all affect the physical effect produced by electromagnetic excitation of the magnetic nanomedia.

[0037] Those skilled in the art will readily understand that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A magnetic nanomaterial magnetothermal-acoustic multi-field coupled neural modulation device, characterized in that, This system utilizes an externally applied excitation coil to generate a pulsed magnetic field, which excites magnetic nanomaterials located at biological target sites in brain functional areas. The magnetic field excitation acts on the magnetic nanomaterials, inducing thermal expansion, magnetothermal-acoustic or magnetic force. Through the ultrasound, magnetic force, and further potential changes caused by magnetothermal, magnetothermal-acoustic, and electromagnetic excitation, neuronal regulation is achieved. The system also utilizes the magnetic, thermal, mechanical, acoustic, and electrical coupling physical effects generated by the electromagnetic nanomaterials at the biological target sites to induce membrane depolarization and induce calcium ion influx in neurons, regulating intracellular calcium signal transduction and thus affecting synaptic plasticity and controlling neural behavior. This allows for precise and controllable regulation of the excitability of neuronal subpopulations in brain regions. The system includes a multi-mode electromagnetic excitation unit, a coil unit capable of focusing electromagnetic field distribution, a coupling conversion unit, and a testing unit. The multi-mode electromagnetic excitation unit generates alternating excitation, modulated signal alternating pulse excitation, and single-pulse excitation at a certain repetition frequency. The unit is used to provide high-power excitation for the coil load. The coupling conversion unit realizes the conversion of electromagnetic excitation to magnetocaloric, magnetocaloric acoustic, and magnetic forces. The test unit is used to test the multi-mode electromagnetic excitation unit, electromagnetic field distribution, physical effects generated by electromagnetic excitation coupling conversion, and further biological effects. The coil unit that can focus the electromagnetic field distribution is used as the load of the multi-mode electromagnetic excitation unit and to generate electromagnetic fields with different morphological distributions. The output terminal of the multi-mode electromagnetic excitation unit is connected to the input terminal of the coupling conversion unit, and the output terminal of the coupling conversion unit is connected to the input terminal of the test unit. The multi-mode electromagnetic excitation unit outputs a current excitation signal to the coupling conversion unit, the coupling conversion unit outputs a signal to the test unit, and the multi-mode electromagnetic excitation unit simultaneously outputs a current signal to the input terminal of the test unit. The test unit outputs a signal to the input terminal of the multi-mode electromagnetic excitation unit according to the tested current parameters to adjust the drive signal of the multi-mode electromagnetic excitation unit.

2. The magnetic nanomaterial magnetothermal-acoustic multi-field coupled neural modulation device according to claim 1, characterized in that: The multi-mode electromagnetic excitation unit consists of a drive signal generator, a power amplifier unit, and an excitation coil matching module. The output terminal of the drive signal generator is connected to the input terminal of the power amplifier unit, and the output terminal of the power amplifier unit is connected to the input terminal of the excitation coil matching module. The drive signal generator outputs a signal to the power amplifier unit, and the power amplifier unit outputs a signal to the excitation coil matching module. Through the excitation coil matching module, the excitation current is applied to the excitation coil.

3. The magnetic nanomaterial magnetothermal-acoustic multi-field coupled neural modulation device according to claim 1, characterized in that: The structure of the coil unit that can focus electromagnetic field distribution is a solenoid coil, a planar coil, an H-type coil, or an array coil. The electromagnetic field distribution characteristics are adjusted according to different coil structures, number of turns, diameter, and wire diameter parameters to generate the required focused electromagnetic field distribution.

4. The magnetic nanomaterial magnetothermal-acoustic multi-field coupled neural modulation device according to claim 1, characterized in that: The coupling conversion unit consists of an excitation coil, a magnetic nanomedium, a calcium indicator, and a head-controlled target. The magnetic nanomedium and the calcium indicator are located in the head-controlled target area. The excitation coil is close to the target area. When the multi-mode electromagnetic excitation unit excites the electromagnetic field through the excitation coil, it generates magnetothermal, magnetothermal acoustic, and magnetic force signals, thereby stimulating biological effects, inducing calcium ion influx, leading to an increase in intracellular cation concentration, and the calcium indicator shows a change in fluorescence intensity.

5. The magnetic nanomaterial magnetothermal-acoustic multi-field coupled neural modulation device according to claim 1, characterized in that: The testing unit includes an excitation source operating status parameter acquisition module, an excitation current detection module, an electromagnetic field tester, a temperature detection module, a magnetothermal-acoustic signal detection module, a micro-magnetic force detection module, a fluorescence imaging system, and a water cooling system. The excitation source operating status parameter acquisition module outputs a signal to the input of the multi-mode electromagnetic excitation unit based on the current parameters measured by the excitation current detection module, adjusting the drive signal of the multi-mode electromagnetic excitation unit. The excitation current detection module detects the current signal generated by the multi-mode electromagnetic excitation unit through the excitation coil. The electromagnetic field tester measures the electromagnetic field distribution characteristics near the excitation coil. The temperature detection module detects the temperature of the head and the magnetic nanomaterial. The magnetothermal-acoustic signal detection module detects the magnetothermal-acoustic signal generated by the excitation coil exciting the magnetic nanomaterial. The micro-magnetic force detection module detects the magnetic force signal generated by the excitation coil exciting the magnetic nanomaterial. The fluorescence imaging system observes the changes in fluorescence intensity caused by calcium ion influx. The water cooling system cools the multi-mode electromagnetic excitation unit and the coil unit.

6. The magnetic nanomaterial magnetothermal-acoustic multi-field coupled neural modulation device according to claim 1, characterized in that: The magnetic nanomediator is a magnetic nanoparticle or a magnetic nanoparticle molecular probe prepared from magnetic nanoparticles. The magnetic nanoparticle molecular probe is composed of magnetic nanoparticles, a carrier, ligands or antibodies. The magnetic nanoparticles include Fe3O4, cobalt-iron-manganese-iron, spherical, and polyhedral magnetic nanoparticles, which are used to enhance the physical effect of electromagnetic field excitation of the target region. The ligands or antibodies are used to bind to the receptors highly expressed in the target region. The electromagnetic and acoustic response characteristics of the magnetic nanoparticles, the thickness and concentration characteristics of the external modification all affect the physical effect produced by electromagnetic excitation of the magnetic nanomediator.

7. The magnetic nanomaterial magnetothermal-acoustic multi-field coupled neural modulation device according to claim 1, characterized in that: By exciting magnetic nanomaterials with an electromagnetic field, the physical effects of the magnetic nanoparticles under electromagnetic excitation, including magnetocaloric, magnetocaloric-acoustic, and magnetic force effects, as well as the induced potential, are utilized to generate biological effects. Specifically, this involves activating the thermosensitive capsaicin receptor TRPV1 in neurons and inducing the release of calcium ions (Ca) into neurons. 2+ Influx of ions leads to an increase in intracellular cation concentration, causing corresponding physiological changes. This study analyzes the effects of the coupled physical effects of different concentrations of magnetic nanoparticles under external excitation on ion channel gating characteristics and synaptic plasticity, establishing the relationship between physical and biological effects. Through calcium indicators and membrane potential observation, the flow of calcium ions inside and outside the cell is controlled, achieving intracellular ion control. By establishing the relationship between physical and biological effects, and combining it with electromagnetic excitation characteristics, the study establishes the relationship between the biological effects induced by magnetic nanoparticles under external excitation and neural regulation. Furthermore, it establishes the relationship between exogenous excitation characteristics, magnetic nanoparticle characteristic parameters, and neural regulation, establishing a spatiotemporal dose-response relationship for activating neural function, thus forming precise neural regulation.

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