A fermentation composition, its preparation method and applications

Abstract

The present invention discloses a fermentation composition, a preparation method thereof and an application, relating to the technical field of cosmetics. The present invention provides a fermentation composition, comprising the following components in parts by weight: 50-70 parts of lysate of bifidobacterium fermentate, 10-30 parts of lysate of lactobacillus fermentate, 10-20 parts of N-acetylglucosamine, and 1-10 parts of glycogen. The present invention provides a fermentation composition, by using the compounding of specific components and controlling the parts by weight of each component in the composition, enabling the components to cooperate with each other and play a synergistic role, comprehensively repairing damaged skin through multiple mechanisms targeting different targets, filling and repairing damaged and aging skin, achieving the purpose of delaying skin aging, with remarkable effects and mild action.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a fermentation composition, a preparation method thereof and an application thereof. Background Art

[0002] Harmful substances such as inhalable particulate matter, sulfur dioxide, nitrogen oxides, carbon monoxide, ozone, etc. in the air not only endanger the health of the human respiratory tract, but also easily cause facial skin to become red and allergic. This situation occurs because the skin surface layer has lost its normal protective function, resulting in the loss of skin moisture content, thus causing skin dryness, flaking or itching. At this time, harmful substances in the air, germs on the skin surface, allergens such as pollen, etc. are easily introduced into the skin through the damaged skin barrier, thereby triggering further skin inflammatory reactions.

[0003] There are quite a number of reasons for skin aging. The subtle effects caused by environmental stress on the skin every day will soon become apparent. When the extreme stress caused by factors such as excessive sun exposure, low temperature, wind, air pollution and emotional fluctuations is superimposed on the damage suffered by the skin, the situation will deteriorate further. Sooner or later, the skin appearance will reflect the consequences of these effects. First, there will be redness or extreme dryness, and then fine lines, wrinkles or skin discoloration can be seen. Fortunately, the skin can detect these problems and take actions to repair the damage suffered. However, this is a race against time. As long as the skin can regenerate in time, the chance of repair is very high. Excessive and continuous exposure to stress will cause the release of free radicals, thereby damaging the skin. At this stage, long-term obvious damage will be inevitable.

[0004] In view of the above problems, there are relatively few anti-pollution cosmetics on the market at present. Coupled with the effects caused by some external factors such as sunburn and after surgery, the protection and repair of damaged skin have become problems that more and more people are eager to solve. Patent CN201910202428.7 provides a lotion for repairing the skin cutin. Although the lotion can inhibit facial skin fungal infections, has an anti-acne effect and can further repair the skin, its formula contains hormones and can only play a role in temporarily relieving skin inflammation, and does not fundamentally solve the problem. In view of the defects of the above-mentioned skin repair products, developing a fermentation composition with mild action, capable of comprehensively repairing the skin and free of hormones is the current research focus. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a fermentation composition, a preparation method thereof and an application thereof.

[0006] To achieve the above object, the technical solution adopted by the present invention is as follows: A fermentation composition, comprising the following components in parts by weight: 50-70 parts of lysate of bifida ferment lysate, 10-30 parts of lysate of lactobacillus ferment, 10-20 parts of N-acetylglucosamine, and 1-10 parts of glycogen.

[0007] Lysate of bifida ferment lysate - is an extract of bifidobacterium, containing metabolites, cytoplasmic components, cell wall components and polysaccharide complexes. This active substance obtained by means of biotechnology can specifically support the skin's own protection and repair mechanisms, and thus can combat premature skin aging. It can restore the balance of the proportions of various mediators, which is very important for the regulation of the immune system. In this way, damaged cells are repaired, keeping the skin looking young and vibrant. Lysate of lactobacillus ferment - contains polypeptide compounds such as lactacin with antibacterial effects, is a natural antibacterial agent, has a sedative and bactericidal effect on the skin, and at the same time adheres to epidermal cells to form a barrier. It is rich in lactic acid and sodium lactate, which are the main water-soluble acids in the natural moisturizing factor (NMF) of the human body, have strong hygroscopicity, effectively enhance the hydration of the stratum corneum, and prevent skin water loss. It contains beneficial skin care ingredients such as amino acids, small peptides, polysaccharides, and vitamin B group, can strengthen the metabolism of the stratum corneum, remove cellular free radicals, inhibit lipid peroxidation, activate the body's repair performance, inhibit the activity of cellular tyrosinase, and has the functions of repair, whitening, and antioxidant. N-acetylglucosamine (NAG) - is the basic constituent unit of many important polysaccharides (such as hyaluronic acid) and glycoproteins inside and outside biological cells, and is an important prerequisite for the synthesis of bifidogenic factors. These substances play important physiological functions such as support, protection, barrier, immunity, and information transmission inside and outside cells. Glycogen - replenishes the glycogen in the skin that decreases with aging, promotes ATP generation and increases cell energy. It promotes the skin to produce hyaluronic acid and ceramides by itself, improves skin moisture, wrinkles and pores. It participates in the expression of antioxidant proteins and inhibits the accumulation of ROS caused by UV and the like.

[0008] Through the synergy of specific lysate of bifida ferment lysate, lysate of lactobacillus ferment, N-acetylglucosamine, and glycogen, the present invention obtains a fermentation composition. Under the selection of the above specific parts by weight, they cooperate with each other and play a synergistic role, repair damaged skin through multiple mechanisms targeting different targets, fill and repair the damaged and aged skin, and can achieve the purpose of delaying skin aging, with remarkable effects and mild actions.

[0009] Preferably, the fermentation composition comprises the following components in parts by weight: 55-65 parts of lysate of bifidobacterium fermentation product, 15-25 parts of lysate of lactobacillus fermentation, 12-17 parts of N-acetylchitosan, and 3-8 parts of glycogen. Further preferably, the fermentation composition comprises the following components in parts by weight: 60 parts of lysate of bifidobacterium fermentation product, 20 parts of lysate of lactobacillus fermentation, 15 parts of N-acetylchitosan, and 5 parts of glycogen.

[0010] After a large number of experimental explorations, the inventors found that when the four components of lysate of bifidobacterium fermentation product, lysate of lactobacillus fermentation, N-acetylchitosan, and glycogen are selected within the above specific parts by weight, the effect of repairing the skin is better. When there are 60 parts of lysate of bifidobacterium fermentation product, 20 parts of lysate of lactobacillus fermentation, 15 parts of N-acetylchitosan, and 5 parts of glycogen, the repair effect reaches the best.

[0011] In addition, the present invention provides the application of the fermentation composition in cosmetics. The cosmetics are one of lotion, cream, emulsion, and essence.

[0012] Furthermore, the present invention provides a cosmetic, which is a lotion and comprises the following components in weight percentages: 1-10% of fermentation composition, 0.5-1% of skin conditioner, 0.1-0.5% of pH regulator, and the balance of water.

[0013] Preferably, the skin conditioner is at least one of 1,2-hexanediol, p-hydroxyacetophenone, sodium chloride, and potassium chloride, and the pH regulator is at least one of arginine, citric acid, sodium citrate, disodium hydrogen phosphate dodecahydrate, and potassium dihydrogen phosphate.

[0014] Preferably, the cosmetic is a lotion and comprises the following components in weight percentages: 6.25% of fermentation composition, 0.75% of sodium chloride, 0.019% of potassium chloride, 0.336% of disodium hydrogen phosphate dodecahydrate, 0.022% of potassium dihydrogen phosphate, and 92.623% of water.

[0015] In addition, the present invention provides a preparation method of the cosmetic, which comprises the following steps:

[0016] (1) Mix the skin conditioner and water, heat to 80-85 °C, disperse evenly, and then cool to 40-45 °C to obtain a mixed solution A;

[0017] (2) Add the pH regulator to the mixed solution A prepared in step (1), mix evenly to obtain a mixed solution B;

[0018] (3) Add the fermentation composition to the mixed solution B prepared in step (2), and mix to obtain the cosmetic.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention provides a fermentation composition. By using a specific component compounding and controlling the weight parts of each component in the composition, the components cooperate with each other to exert a synergistic effect, comprehensively repair damaged skin through multiple mechanisms for different targets, fill and repair the damaged and aging skin, and achieve the purpose of delaying skin aging, with remarkable effects and mild actions. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a morphological result diagram of the cytotoxicity test of Sample 1;

[0021] Figure 2 It is a test result diagram of tissue morphology;

[0022] Figure 3 It is a test result diagram of the barrier-related protein LOR. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with the drawings and specific embodiments. In the embodiments, unless otherwise specified, the experimental methods used are all conventional methods, and the materials, reagents, etc. used, unless otherwise specified, can all be obtained from commercial channels.

[0024] Specifically, the raw materials used in the present invention are as follows:

[0025] Lyophilized product of Saccharomyces boulardii fermentation product, manufacturer Huzhou Ketin Biotechnology Co., Ltd., model BFL;

[0026] Lyophilized product of Lactobacillus fermentation, manufacturer Huzhou Ketin Biotechnology Co., Ltd.;

[0027] N-acetyl-D-glucosamine, manufacturer Jiangsu Aoxin Bioengineering Co., Ltd.;

[0028] Glycogen, manufacturer GLICO NUTRITION CO., LTD., model BIOGLYCOGEN;

[0029] Hydrolyzed yeast, manufacturer KATAKURA INDUSTRIES Co., Ltd., model LEVURE GL;

[0030] Extract of Lactobacillus / soybean fermentation product, manufacturer Technoble Co., Ltd., model PESTICIDE-FREE SOYMILK-FF;

[0031] Chitosan, manufacturer Tai'an Jiangzhou Biotechnology Co., Ltd.;

[0032] Chitosan, manufacturer: Jiangsu Pulesi Biotechnology Co., Ltd.;

[0033] The present invention sets up examples and comparative examples. The weight ratio relationships of the lysate of Saccharomyces boulardii fermentation product, the lysate of Lactobacillus fermentation, N-acetylglucosamine, and glycogen in the fermentation compositions of the examples and comparative examples are shown in Tables 1 and 2.

[0034] The softeners of the examples and comparative examples contain the fermentation compositions described in Tables 1 and 2, and include the following components in weight percentages: fermentation composition 6.25%, sodium chloride 0.75%, potassium chloride 0.019%, disodium hydrogen phosphate dodecahydrate 0.336%; potassium dihydrogen phosphate 0.022%, water 92.623%.

[0035] The preparation method of the softeners of the examples and comparative examples includes the following steps:

[0036] (1) Mix the skin conditioner and water, heat to 80 - 85°C, disperse evenly, and then cool to 40 - 45°C to obtain a mixed solution A;

[0037] (2) Add the pH regulator to the mixed solution A prepared in step (1), mix evenly to obtain a mixed solution B;

[0038] (3) Add the fermentation composition to the mixed solution B prepared in step (2), and mix to obtain the softener.

[0039] Table 1

[0040]

[0041] Table 2

[0042]

[0043] Performance Test 1 Soothing Effect Test

[0044] Test subjects: 110 volunteers aged 25 - 45 with skin inflammation such as peeling, erythema, and itching were randomly divided into 11 groups, with 10 people in each group.

[0045] Test process: The test time was 30 days. It was applied once in the morning and once in the evening every day. The products prepared in Examples 1 - 5 and Comparative Examples 1 - 6 of the present invention were applied at 0.05 mg / cm 2 , massaged until absorbed. Other products were not used during this period. At the same time, a blank group (without applying any product) was set up, and the skin conditions were observed 7 days, 14 days, and 30 days after use.

[0046] Test results: The skin conditions were divided into 4 levels: severe erythema eczema (9 - 10 points), moderate erythema eczema (6 - 8 points), mild erythema eczema (3 - 5 points), and normal (1 - 2 points).

[0047] The test results are shown in Table 3.

[0048] Table 3 Test Results of Soothing Effect

[0049] Group 0 days 7 days 14 days 30 days Example 1 9 2 - - Example 2 9 3 2 - Example 3 9 4 2 - Example 4 9 5 4 2 Example 5 9 5 4 1 Comparative Example 1 9 6 5 3 Comparative Example 2 9 8 6 5 Comparative Example 3 9 8 6 5 Comparative Example 4 9 7 5 4 Comparative Example 5 9 6 5 3 Comparative Example 6 9 7 5 3 Blank group 9 9 9 10

[0050] As can be seen from the above table, the soft skin lotion prepared in Examples 1-5 of the present invention has changed from severe erythema eczema to mild after 7 days of use. Moreover, the weight parts of the lysate of Saccharomyces boulardii fermentation product, the lysate of Lactobacillus fermentation, N-acetylglucosamine, and glycogen have a great influence on the soothing and repairing effect of the prepared fermentation composition. When the lysate of Saccharomyces boulardii fermentation product is 60 parts, the lysate of Lactobacillus fermentation is 20 parts, N-acetylglucosamine is 15 parts, and glycogen is 5 parts, the repair effect reaches the best, and it returns to normal only after 7 days.

[0051] For the soft skin lotion prepared in Comparative Examples 1-6, although it also has a certain effect in terms of use effect, generally speaking, the effect is slow to appear, and mild erythema eczema still appears after one month of use.

[0052] Performance Test 2

[0053] The fermentation compositions obtained by naming Example 1 as Lyfida2.0 and Comparative Example 1 as Lyfida1.0 were sent to Shaanxi Boxi General Testing Technology Co., Ltd. (CMA (Certificate of Qualification for Inspection and Testing Institutions)) Certificate No.: 202714340013 for maintenance efficacy testing.

[0054] Maintenance efficacy test. [[ID=2E]]

[0055] Detection model: SLS-stimulated epidermal model EpiKutis; When the skin contacts some test substances with strong irritation or ultraviolet rays, etc., it will cause clinical acute damage to the skin barrier, resulting in skin dryness and erythema. The anionic surfactant sodium lauryl sulfate (SLS) has amphiphilic (hydrophilic and lipophilic) characteristics. After contacting the skin at a relatively high concentration, it can damage the skin barrier and further damage living cells, especially the lipid components and cell membranes in the barrier. In this experiment, the in vitro reconstituted epidermal model EpiKutis was used as the research object, and an injury model was established by SLS stimulation, and then the product was treated. The changes in tissue morphology, the content of the barrier-related protein LOR, and the epidermal water loss rate were targeted to detect the repair efficacy of cosmetic raw materials.

[0056] Detection indicators: Tissue morphology; Barrier-related protein: Content of the cornified envelope protein LOR; Change in epidermal water loss rate TEWL.

[0057] Tissue morphology - Tissue morphology is the analysis of the microscopic physiological structure of tissues after HE staining. Through histological morphology of the model, the changes in the skin barrier under different treatment conditions can be observed. After stimulation with SLS, the stratum corneum of the epidermal model EpiKutis becomes loose, and the number of living cells in the viable cell layer decreases significantly and is arranged disorderly. However, the application of the sample can make the stratum corneum regain its tightness and increase in thickness; the number of living cells in the viable cell layer increases significantly, the thickness increases, and the cell arrangement is more orderly. The epidermal tissue resumes its original morphology, indicating that the sample has a certain repair effect.

[0058] Loricrin (LOR) - Loricrin (LOR) is a key component in the CE assembly process, accounting for approximately 80% of the CE composition content, and plays a crucial role in strengthening the skin barrier. An increase in its expression can enhance the skin's permeability barrier. The application of the sample increases the expression level of LOR and strengthens the skin barrier structure, indicating that the sample has a certain repair effect.

[0059] Change in transepidermal water loss (TEWL) - Transepidermal water loss (TEWL), also known as percutaneous water loss, refers to the rate of water loss through the epidermis per unit time. The larger the TEWL, the more water is lost per unit time, the weaker the skin's moisturizing ability, and the weaker the skin barrier protection ability.

[0060] Detection methods: Tissue morphology - H&E, Loricrin (LOR) - IF, Change in transepidermal water loss (TEWL) - Test with TW24 instrument.

[0061] Test process: Set up a blank control group (BC), a negative control group (NC), a positive control group (PC), and a sample group (Sample).

[0062] The blank control group (BC) is not treated with anything.

[0063] The negative control group (NC) is induced with 0.1% SLS.

[0064] The positive control group (PC) is given a sample concentration of 50 μM and induced with 0.1% SLS.

[0065] Sample group 1 is Lyfida 2.0 and is induced with 0.1% SLS.

[0066] Sample group 2 is Lyfida 1.0 and is induced with 0.1% SLS.

[0067] Test results: As Figure 2-3 shown.

[0068] The test results of tissue morphology are as Figure 2As shown, compared with the BC group, the living cell layer of the epidermal model in the NC group became thinner (arrow), and vacuolation occurred (circle), indicating that the SLS stimulation condition was effective. Compared with the NC group, the living cell layer of the model in the PC group thickened, and cell damage was significantly improved, indicating that the positive control test was effective this time. Compared with the NC group, the damage in samples 1 (Lyfida 2.0)-6.25% and samples 2 (Lyfida 1.0)-6.25% was significantly improved. The application of the samples restored the tightness of the stratum corneum and increased its thickness; the number of living cells in the living cell layer increased significantly, the thickness increased, and the cell arrangement became more orderly.

[0069] The test results of the barrier-related protein LOR are as Figure 3 shown in Table 4. Compared with the BC group, the LOR content in the NC group decreased significantly, indicating that the SLS stimulation condition was effective. Compared with the NC group, the LOR content in the PC group increased significantly, indicating that the positive control test was effective this time. Compared with the NC group, the LOR content in the sample 1 (Lyfid a 2.0)-6.25% group increased significantly. Sample 1 significantly improved the model damage. The application of the sample increased the fluorescence distribution area significantly, the fluorescence intensity increased, and the LOR protein content increased significantly (P<0.01). Figure 2 The original figure was in color, and the whitened part showed the original fluorescence distribution.

[0070] Table 4

[0071]

[0072] Table 5

[0073]

[0074]

[0075] The test results of the change in transepidermal water loss rate (TEWL) are shown in Table 5. Compared with the BC group, the TEWL value in the NC group increased significantly, indicating that the SLS stimulation condition was effective this time. Compared with the NC group, the TEWL value in the PC group decreased significantly, indicating that the positive control test was effective. Compared with the NC group, the TEWL values in the sample 1 (Lyfida 2.0)-6.25% and sample 2 (Lyfida 1.0)-6.25% groups decreased significantly.

[0076] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A fermentation composition, characterized in that The invention is composed of the following components in parts by weight: 60 parts of bifid yeast fermentation lysate, 20 parts of lactobacillus fermentation lysate, 15 parts of acetyl glucosamine and 5 parts of glycogen.

2. Use of the fermentation composition according to claim 1 in preparing cosmetics.

3. A cosmetic, characterized in that: The cosmetic is a toner, comprising the following components in percentage by weight: 1-10% of the fermented composition of claim 1, 0.5-1% of a skin conditioner, 0.1-0.5% of a pH regulator, and the balance being water.

4. The cosmetic according to claim 3, wherein The skin conditioning agent is at least one of 1,2-hexanediol, p-hydroxyacetophenone, sodium chloride, and potassium chloride; the pH regulator is at least one of arginine, citric acid, sodium citrate, disodium hydrogen phosphate dodecahydrate, and potassium dihydrogen phosphate.

5. The cosmetic according to claim 4, wherein The cosmetic is a toner, comprising the following components in percentage by weight: The fermentation composition of claim 1 comprises 6.25%, 0.75% sodium chloride, 0.019% potassium chloride, 0.336% disodium hydrogen phosphate dodecahydrate, 0.022% potassium dihydrogen phosphate, and 92.623% water.

6. A method for preparing a cosmetic according to any one of claims 3 to 5, characterized in that: The steps include: (1) Mix the skin conditioner and water, heat to 80-85°C, disperse evenly, and then cool to 40-45°C to obtain a mixed solution A; (2) adding a pH regulator to the mixed solution A prepared in step (1), mixing the mixture evenly, and obtaining a mixed solution B; (3) Adding the fermentation composition to the mixed solution B prepared in step (2), and mixing to obtain the cosmetic.

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