A method for preparing white shrimp granular biological feed fermented by Clostridium butyricum

Abstract

The invention discloses a method for preparing white shrimp granular biological feed fermented by clostridium butyricum, comprising the following steps: step 1, preparing a special formula feed; step 2, aerobic solid fermentation; step 3, anaerobic solid fermentation; step 4, packaging the granular feed after anaerobic fermentation, and storing it in sealed plastic bags according to specification requirements. The invention activates an aerobic fermentation agent, sprays it on the granular feed in an atomized manner, mixes and ferments it evenly, sprays the atomized clostridium butyricum on the surface of the fermented feed in an anaerobic mixer, and forms a completely anaerobic environment for fermentation through a vacuum filtration process, so that nutrients that are not easily absorbed in a basic feed can be decomposed and converted into small molecules such as glucose and amino acids that are easily digested and absorbed in a longer-term, stable and rapid manner, anti-nutritional factors in feed raw materials can be degraded or eliminated, the bacterial environment of the shrimp intestine can be adjusted, and the shrimp immunity can be improved.

Description

Technical Field

[0001] The invention relates to the technical field of biological fermentation, and in particular to a method for preparing white shrimp granular biological feed fermented by Clostridium butyricum. Background Art

[0002] The current method of preparing biological feed mainly uses composite bacteria (Bacillus subtilis, lactic acid bacteria, yeast, etc.) as fermentation agents, and the dosage form is mainly powder. Its main goal is to pre-digest feed raw materials or remove toxins, and at the same time obtain metabolites such as small peptides and bio-acids.

[0003] The biological feed metabolites obtained by the existing technology are single, but it is impossible to obtain butyric acid, an important intestinal repair factor and food attractant, and it is impossible to obtain live Clostridium butyricum with strong stress resistance, which requires users to add extra Clostridium butyricum when using it. However, this solution often requires harsh conditions and has low biological potency. Therefore, in order to improve the application efficiency and efficacy of Clostridium butyricum, the present invention provides a method for preparing white shrimp granular biological feed fermented by Clostridium butyricum. Summary of the invention

[0004] In view of the defects of the prior art, the purpose of the present invention is to provide a method for preparing a granular biological feed of Penaeus vannamei fermented with Clostridium butyricum to solve the problems raised in the above background technology.

[0005] The present invention solves the technical problem by adopting the following technical solution:

[0006] The present invention provides a method for preparing a white shrimp granular biological feed fermented by Clostridium butyricum, comprising the following steps:

[0007] Step 1: Preparation of special formula feed: extruding and granulating the basic feed and the nutritional factors required by the probiotics to obtain pellet feed;

[0008] Step 2: Aerobic solid fermentation: After activating the aerobic fermentation agent, spray it on the pellet feed, mix it evenly, and place the pellet feed in a constant temperature and humidity room for fermentation;

[0009] Step 3, anaerobic solid fermentation: the pellet feed that has completed aerobic fermentation in step 2 is collected and placed in an anaerobic mixer, and Clostridium butyricum activation solution is inoculated and fermented in the anaerobic mixer;

[0010] Step 4: Pack the pellet feed after anaerobic fermentation and store it in sealed plastic bags according to specification requirements.

[0011] Preferably, the nutritional factors in step one include the following raw materials in parts by weight: 8-12 parts of defatted fish meal, 10-20 parts of soybean meal, 10-20 parts of peanut meal, 4-8 parts of flour, 5-10 parts of shrimp shell powder, 3-6 parts of cuttlefish paste, 0.5-1.5 parts of grape seed meal oil, 1-3 parts of sucrose, 0.1-1 parts of monocalcium phosphate, 0.1-1 parts of monopotassium phosphate, and 0.5-1.5 parts of monopotassium sulfate.

[0012] Preferably, the expanded particle size in step 1 is 0.5-1.0 mm.

[0013] Preferably, the medium aerobic fermentation agent in step 2 is composed of Bacillus subtilis, Lactobacillus acidophilus, yeast and protease, and the mass ratio of Bacillus subtilis, Lactobacillus acidophilus, yeast and protease is 1-1.2:0.5-0.6:0.1-0.2:0.2-0.3.

[0014] Preferably, the aerobic fermentation agent activation method in step 2 comprises the following steps: adding sucrose and 30-50°C water to a strain expansion tank and stirring evenly, then adding Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease and stirring evenly and then standing for 60-120 minutes; finally adding modified nano-silicon dioxide and a loading treatment agent and stirring sufficiently to obtain; wherein the weight of the modified nano-silicon dioxide and the loading treatment agent is 5-10% and 1-5% of the total amount of Bacillus subtilis;

[0015] The modification method of modified nano silicon dioxide is as follows:

[0016] Adding nano-silicon dioxide to a 5% sodium dodecyl sulfate solution at a weight ratio of 1:5, then adding a chitosan aqueous solution having a mass fraction of 10-15% and a total amount of 2-5% of the nano-silicon dioxide, followed by adding 2-5% of hydroxyacetic acid and 1-5% of dodecylphenol, stirring sufficiently, and finally washing with water and drying to obtain modified nano-silicon dioxide;

[0017] The loading treatment agent is hydroxyapatite which is calcined at 300-320°C for 10-20min, cooled to 40-45°C, evenly dispersed in 3-5 times the mass fraction of 5% hydrochloric acid solution, and finally washed with water and dried to obtain.

[0018] Preferably, the temperature in step 2 is 30-40° C. and the fermentation time is 24-72 hours.

[0019] Preferably, the fermentation step in step three is: spraying the Clostridium butyricum activation liquid on the surface of the feed placed in an anaerobic mixer after atomization, the anaerobic mixer forms a completely anaerobic environment through a vacuum filtration process, and the product is obtained after standing solid fermentation.

[0020] Preferably, the oxygen partial pressure in the anaerobic mixer is 0.

[0021] Preferably, the temperature of the static solid fermentation is 35-45°C, and the time of the static solid fermentation is 48-96h.

[0022] Preferably, the content of Clostridium butyricum in the feed in step 4 is not less than 1 billion CFU / g.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The invention activates an aerobic fermentation agent, sprays it on a granular feed, mixes and ferments it uniformly, sprays the surface of the fermented feed with atomized Clostridium butyricum in an anaerobic mixer, and forms a completely anaerobic environment for fermentation through a vacuum filtration process. Compared with directly added free live bacteria, the invention can decompose the nutrients that are not easily absorbed in the basic feed into small molecules such as glucose and amino acids that are easily digested and absorbed in a longer-term, stable and rapid manner, degrade or eliminate anti-nutritional factors in the feed raw materials, regulate the intestinal bacterial environment of shrimps, produce a large amount of active substances during the fermentation process, and improve the immunity of shrimps to resist various bacterial diseases. The invention adds modified nano-silicon dioxide and a load treatment agent to the aerobic fermentation agent activation method, and adopts the synergistic effect of the two to enhance the fermentation effect of the product raw materials. This is because the nano-silicon dioxide has a high specific surface area. After being modified by the method of the invention, it can be synergistically matched with hydroxyapatite to enhance the effect. The porous adsorption performance of hydroxyapatite acts on the aerobic fermentation agent to promote the fermentation efficiency of the product and improve the effect of the product.

[0025] The present invention adopts a two-step method to ferment feed and prepare compound feed, which not only greatly improves the application efficiency of Clostridium butyricum in the breeding end, but also enhances the efficacy of Clostridium butyricum in the compound feed, and simplifies the process of feed preparation for farmers. DETAILED DESCRIPTION

[0026] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] The present invention of this embodiment provides a method for preparing a granular biological feed of Penaeus vannamei fermented with Clostridium butyricum, comprising the following steps:

[0028] Step 1: Preparation of special formula feed: extruding and granulating the basic feed and the nutritional factors required by the probiotics to obtain pellet feed;

[0029] Step 2: Aerobic solid fermentation: After activating the aerobic fermentation agent, spray it on the pellet feed, mix it evenly, and place the pellet feed in a constant temperature and humidity room for fermentation;

[0030] Step 3, anaerobic solid fermentation: the pellet feed that has completed aerobic fermentation in step 2 is collected and placed in an anaerobic mixer, and Clostridium butyricum activation solution is inoculated and fermented in the anaerobic mixer;

[0031] Step 4: Pack the pellet feed after anaerobic fermentation and store it in sealed plastic bags according to specification requirements.

[0032] The nutritional factors in step 1 of this embodiment include the following raw materials in parts by weight: 8-12 parts of defatted fish meal, 10-20 parts of soybean meal, 10-20 parts of peanut meal, 4-8 parts of flour, 5-10 parts of shrimp shell powder, 3-6 parts of cuttlefish paste, 0.5-1.5 parts of grape seed meal oil, 1-3 parts of sucrose, 0.1-1 parts of calcium dihydrogen phosphate, 0.1-1 parts of potassium dihydrogen phosphate, and 0.5-1.5 parts of potassium dihydrogen sulfate.

[0033] In step 1 of this embodiment, the expanded particle size is 0.5-1.0 mm.

[0034] In step 2 of this embodiment, the aerobic fermentation agent is composed of Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease, and the mass ratio of Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease is 1-1.2: 0.5-0.6: 0.1-0.2: 0.2-0.3.

[0035] The aerobic fermentation agent activation method in step 2 of this embodiment comprises the following steps: adding sucrose and 30-50° C. water to a strain expansion tank and stirring evenly, then adding Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease and stirring evenly and then standing for 60-120 minutes; finally adding modified nano-silicon dioxide and a loading treatment agent and stirring sufficiently to obtain; wherein the weight of the modified nano-silicon dioxide and the loading treatment agent is 5-10% and 1-5% of the total amount of Bacillus subtilis;

[0036] The modification method of modified nano silicon dioxide is as follows:

[0037] Adding nano-silicon dioxide to a 5% sodium dodecyl sulfate solution at a weight ratio of 1:5, then adding a chitosan aqueous solution having a mass fraction of 10-15% and a total amount of 2-5% of the nano-silicon dioxide, followed by adding 2-5% of hydroxyacetic acid and 1-5% of dodecylphenol, stirring sufficiently, and finally washing with water and drying to obtain modified nano-silicon dioxide;

[0038] The loading treatment agent is hydroxyapatite which is calcined at 300-320°C for 10-20min, cooled to 40-45°C, evenly dispersed in 3-5 times the mass fraction of 5% hydrochloric acid solution, and finally washed with water and dried to obtain.

[0039] In step 2 of this embodiment, the temperature is 30-40° C. and the fermentation time is 24-72 hours.

[0040] The fermentation step in step three of this embodiment is: spraying the Clostridium butyricum activation liquid on the surface of the feed placed in the anaerobic mixer after atomization, the anaerobic mixer forms a completely anaerobic environment through a vacuum filtration process, and the product is obtained after standing solid fermentation.

[0041] The oxygen partial pressure in the anaerobic mixer of this embodiment is 0.

[0042] The static solid fermentation temperature of this embodiment is 35-45°C, and the static solid fermentation time is 48-96h

[0043] In step 4 of this embodiment, the content of Clostridium butyricum in the feed is not less than 1 billion CFU / g.

[0044] Example 1.

[0045] The present invention of this embodiment provides a method for preparing a granular biological feed of Penaeus vannamei fermented with Clostridium butyricum, comprising the following steps:

[0046] Step 1: Preparation of special formula feed: extruding and granulating the basic feed and the nutritional factors required by the probiotics to obtain pellet feed;

[0047] Step 2: Aerobic solid fermentation: After activating the aerobic fermentation agent, spray it on the pellet feed, mix it evenly, and place the pellet feed in a constant temperature and humidity room for fermentation;

[0048] Step 3, anaerobic solid fermentation: the pellet feed that has completed aerobic fermentation in step 2 is collected and placed in an anaerobic mixer, and Clostridium butyricum activation solution is inoculated and fermented in the anaerobic mixer;

[0049] Step 4: Pack the pellet feed after anaerobic fermentation and store it in sealed plastic bags according to specification requirements.

[0050] The nutritional factors in step 1 of this embodiment include the following raw materials in parts by weight: 8 parts of defatted fish meal, 10 parts of soybean meal, 10 parts of peanut meal, 4 parts of flour, 5 parts of shrimp shell powder, 3 parts of squid paste, 0.5 parts of grape seed meal oil, 1 part of sucrose, 0.1 parts of calcium dihydrogen phosphate, 0.1 parts of potassium dihydrogen phosphate, and 0.5 parts of potassium dihydrogen sulfate.

[0051] In step 1 of this embodiment, the expanded particle size is 0.5 mm.

[0052] In step 2 of this embodiment, the aerobic fermentation agent is composed of Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease, and the mass ratio of Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease is 1:0.5:0.1:0.2.

[0053] The aerobic fermentation agent activation method in step 2 of this embodiment includes the following steps: adding sucrose and 30° C. water to a strain expansion tank and stirring evenly, then adding Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease and stirring evenly and then standing for 60 minutes; finally adding modified nano-silicon dioxide and a loading treatment agent and stirring sufficiently to obtain; wherein the weight of the modified nano-silicon dioxide and the loading treatment agent is 5% and 1% of the total amount of Bacillus subtilis;

[0054] The modification method of modified nano silicon dioxide is as follows:

[0055] Add nano-silica to a 5% sodium dodecyl sulfate solution at a weight ratio of 1:5, then add a 10% chitosan aqueous solution with a total amount of 2% of the nano-silica, then add 2% of hydroxyacetic acid and 1% dodecylphenol with a total amount of the chitosan aqueous solution, stir well, and finally wash with water and dry to obtain modified nano-silica;

[0056] The loading treatment agent is hydroxyapatite which is calcined at 300°C for 10 minutes, cooled to 40°C, evenly dispersed in a 3 times mass fraction 5% hydrochloric acid solution, and finally washed with water and dried to obtain.

[0057] In step 2 of this embodiment, the temperature is 30° C. and the fermentation time is 24 h.

[0058] The fermentation step in step three of this embodiment is: spraying the Clostridium butyricum activation liquid on the surface of the feed placed in the anaerobic mixer after atomization, the anaerobic mixer forms a completely anaerobic environment through a vacuum filtration process, and the product is obtained after standing solid fermentation.

[0059] In the anaerobic mixer of this embodiment, the oxygen partial pressure is 0, the fermentation temperature is 35° C., and the fermentation time is 48 hours.

[0060] In step 4 of this embodiment, the content of Clostridium butyricum in the feed is not less than 1 billion CFU / g.

[0061] Example 2.

[0062] The present invention of this embodiment provides a method for preparing a granular biological feed of Penaeus vannamei fermented with Clostridium butyricum, comprising the following steps:

[0063] Step 1: Preparation of special formula feed: extruding and granulating the basic feed and the nutritional factors required by the probiotics to obtain pellet feed;

[0064] Step 2: Aerobic solid fermentation: After activating the aerobic fermentation agent, spray it on the pellet feed, mix it evenly, and place the pellet feed in a constant temperature and humidity room for fermentation;

[0065] Step 3, anaerobic solid fermentation: the pellet feed that has completed aerobic fermentation in step 2 is collected and placed in an anaerobic mixer, and Clostridium butyricum activation solution is inoculated and fermented in the anaerobic mixer;

[0066] Step 4: Pack the pellet feed after anaerobic fermentation and store it in sealed plastic bags according to specification requirements.

[0067] The nutritional factors in step 1 of this embodiment include the following raw materials in parts by weight: 12 parts of defatted fish meal, 20 parts of soybean meal, 20 parts of peanut meal, 8 parts of flour, 10 parts of shrimp shell powder, 6 parts of cuttlefish paste, 1.5 parts of grape seed meal oil, 3 parts of sucrose, 1 part of calcium dihydrogen phosphate, 1 part of potassium dihydrogen phosphate, and 1.5 parts of potassium dihydrogen sulfate.

[0068] In step 1 of this embodiment, the expanded particle size is 1.0 mm.

[0069] In step 2 of this embodiment, the aerobic fermentation agent is composed of Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease, and the mass ratio of Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease is 1.2:0.6:0.2:0.3.

[0070] The aerobic fermentation agent activation method in step 2 of this embodiment includes the following steps: adding sucrose and 50° C. water to a strain expansion tank and stirring evenly, then adding Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease and stirring evenly and then standing for 120 minutes; finally adding modified nano-silicon dioxide and a loading treatment agent and stirring sufficiently to obtain; wherein the weight of the modified nano-silicon dioxide and the loading treatment agent is 10% and 5% of the total amount of Bacillus subtilis;

[0071] The modification method of modified nano silicon dioxide is as follows:

[0072] Add nano-silica to a 5% sodium dodecyl sulfate solution at a weight ratio of 1:5, then add a 15% chitosan aqueous solution with a total amount of 5% of the nano-silica, then add 5% of hydroxyacetic acid and 5% of dodecylphenol with a total amount of the chitosan aqueous solution, stir well, and finally wash with water and dry to obtain modified nano-silica;

[0073] The loading treatment agent is hydroxyapatite which is calcined at 320°C for 20 minutes, cooled to 45°C, evenly dispersed in a 5% hydrochloric acid solution with a mass fraction of 5, and finally washed with water and dried to obtain.

[0074] In step 2 of this embodiment, the temperature is 40° C. and the fermentation time is 72 h.

[0075] The fermentation step in step three of this embodiment is: spraying the Clostridium butyricum activation liquid on the surface of the feed placed in the anaerobic mixer after atomization, the anaerobic mixer forms a completely anaerobic environment through a vacuum filtration process, and the product is obtained after standing solid fermentation.

[0076] In the anaerobic mixer of this embodiment, the oxygen partial pressure is 0, the fermentation temperature is 45° C., and the fermentation time is 96 hours.

[0077] In step 4 of this embodiment, the content of Clostridium butyricum in the feed is not less than 1 billion CFU / g.

[0078] Example 3.

[0079] The present invention of this embodiment provides a method for preparing a granular biological feed of Penaeus vannamei fermented with Clostridium butyricum, comprising the following steps:

[0080] Step 1: Preparation of special formula feed: extruding and granulating the basic feed and the nutritional factors required by the probiotics to obtain pellet feed;

[0081] Step 2: Aerobic solid fermentation: After activating the aerobic fermentation agent, spray it on the pellet feed, mix it evenly, and place the pellet feed in a constant temperature and humidity room for fermentation;

[0082] Step 3, anaerobic solid fermentation: the pellet feed that has completed aerobic fermentation in step 2 is collected and placed in an anaerobic mixer, and Clostridium butyricum activation solution is inoculated and fermented in the anaerobic mixer;

[0083] Step 4: Pack the pellet feed after anaerobic fermentation and store it in sealed plastic bags according to specification requirements.

[0084] The nutritional factors in step 1 of this embodiment include the following raw materials in parts by weight: 10 parts of defatted fish meal, 15 parts of soybean meal, 15 parts of peanut meal, 6 parts of flour, 7 parts of shrimp shell powder, 4.5 parts of squid paste, 1 part of grape seed meal oil, 2 parts of sucrose, 0.55 parts of monocalcium phosphate, 0.5 parts of monopotassium phosphate, and 1 part of monopotassium sulfate.

[0085] In step 1 of this embodiment, the expanded particle size is 0.8 mm.

[0086] In step 2 of this embodiment, the aerobic fermentation agent is composed of Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease, and the mass ratio of Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease is 1.1:0.55:0.15:0.25.

[0087] The aerobic fermentation agent activation method in step 2 of this embodiment includes the following steps: adding sucrose and 40° C. water to a strain expansion tank and stirring evenly, then adding Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease and stirring evenly and then standing for 90 minutes; finally adding modified nano-silicon dioxide and a loading treatment agent and stirring sufficiently to obtain; wherein the weight of the modified nano-silicon dioxide and the loading treatment agent is 7.5% and 3.5% of the total amount of Bacillus subtilis;

[0088] The modification method of modified nano silicon dioxide is as follows:

[0089] Add nano-silica to a 5% sodium dodecyl sulfate solution at a weight ratio of 1:5, then add a 12% chitosan aqueous solution with a total amount of 3.5% of the nano-silica, then add 3.5% of hydroxyacetic acid and 3% of dodecylphenol with a total amount of the chitosan aqueous solution, stir well, and finally wash with water and dry to obtain modified nano-silica;

[0090] The loading treatment agent is hydroxyapatite which is calcined at 310°C for 15 minutes, cooled to 42°C, evenly dispersed in a 4 times mass fraction 5% hydrochloric acid solution, and finally washed with water and dried to obtain.

[0091] In step 2 of this embodiment, the temperature is 35° C. and the fermentation time is 48 h.

[0092] The fermentation step in step three of this embodiment is: spraying the Clostridium butyricum activation liquid on the surface of the feed placed in the anaerobic mixer after atomization, the anaerobic mixer forms a completely anaerobic environment through a vacuum filtration process, and the product is obtained after standing solid fermentation.

[0093] In the anaerobic mixer of this embodiment, the oxygen partial pressure is 0, the fermentation temperature is 40° C., and the fermentation time is 72 hours.

[0094] In step 4 of this embodiment, the content of Clostridium butyricum in the feed is not less than 1 billion CFU / g.

[0095] Comparative Example 1.

[0096] The difference from Example 3 is that no nutritional factors were added.

[0097] Comparative Example 2.

[0098] The difference from Example 3 is that in step 2, the aerobic fermentation agent is not activated and directly sprayed.

[0099] Comparative Example 3.

[0100] The difference from Example 3 is that in step 2, the temperature is kept at 50° C. and the fermentation time is 60 h.

[0101] Comparative Example 4.

[0102] The difference from Example 3 is that the fermentation temperature in step 3 is 30°C.

[0103] Comparative Example 5.

[0104] The difference from Example 3 is that Clostridium butyricum was not added.

[0105] Examples 1-3 and Comparative Examples 1-5 were made into granular biological feeds for performance testing;

[0106] 1800 healthy juvenile Penaeus vannamei shrimps of 15-20 days old were randomly selected and divided into 9 groups, 200 in each group, and the first 8 groups were divided into A / B / C / D / E / F / G / H, the 9th group was blank control group I, group A used the pellet feed made by embodiment 1, group B used the pellet feed made by embodiment 2, and so on, wherein group I was fed with ordinary feed, and the rearing period of 9 groups of Penaeus vannamei shrimps was 40 days, and 9 groups of Penaeus vannamei shrimps did not use drugs throughout the whole process, and other breeding conditions were the same. The mortality rate and individual average weight of each group of Penaeus vannamei shrimps were recorded after 2 months, and the results are shown in the table below.

[0107]

[0108] It can be seen from Examples 1-3 that after using the granular biological feed prepared by the present invention, the survival rate of the shrimps in Group AC was significantly higher than the mortality rate, with the highest average weight being 14.25 g and the lowest being 12.32 g, thus highlighting that the preparation method of the granular biological feed of Penaeus vannamei fermented with Clostridium butyricum of the present invention can increase the yield of shrimps;

[0109] It can be seen from Comparative Example 1 and Example 3 that the absence of nutritional factors greatly reduces the absorption of nutrients in the feed by the shrimp, reduces its own weight, and thus reduces the survival rate. Compared with the biological feed prepared by the present invention, its efficacy is significantly reduced;

[0110] It was found from Comparative Examples 2-3 that the failure to activate the aerobic fermentation agent and the increase in fermentation temperature after activation greatly reduced the activity of the bacteria in the aerobic fermentation agent, causing the concentration of metabolites such as small peptides and bio-acids in the feed to decrease, seriously affecting the digestion of the shrimp after eating, thereby reducing the average weight of the shrimp.

[0111] In addition, it can be seen from Comparative Examples 4-5 that the growth of shrimps is slow and the survival rate is reduced when Clostridium butyricum is not added during the process of making the biological feed and the fermentation temperature is lowered after Clostridium butyricum is added.

[0112] Comparative experimental example 1.

[0113] The difference from Example 3 is that no modified nano-silica or loading treatment agent is added to the aerobic fermentation agent.

[0114] Comparative Experiment Example 2

[0115] The difference from Example 3 is that the modified nano-silicon dioxide is replaced by nano-silicon dioxide.

[0116] Comparative Experiment 3

[0117] The difference from Example 3 is that no loading treatment agent was added.

[0118] Comparative Experiment Example 4

[0119] The difference from Example 3 is that no chitosan aqueous solution is added in the preparation of the modified nano-silica.

[0120]

[0121] After comparative experimental tests, it was found that the addition of modified nano-silica and loading treatment agents can achieve the effect of synergistically enhancing the product.

[0122] The innovation of the present invention is:

[0123] The invention activates an aerobic fermentation agent, sprays it on a granular feed, mixes and ferments it evenly, sprays the fermented feed with atomized Clostridium butyricum on its surface in an anaerobic mixer, and forms a completely anaerobic environment for fermentation through a vacuum filtration process. Compared with directly added free live bacteria, the invention can decompose and convert the nutrients that are not easily absorbed in the basic feed into small molecules such as glucose and amino acids that are easily digested and absorbed for a longer period of time, stably and quickly, degrade or eliminate anti-nutritional factors in the feed raw materials, regulate the intestinal bacterial environment of shrimps, generate a large amount of active substances during the fermentation process, improve the immunity of shrimps, and resist various bacterial diseases.

[0124] The present invention adopts a two-step method to ferment feed and prepare compound feed, which not only greatly improves the application efficiency of Clostridium butyricum in the breeding end, but also enhances the efficacy of Clostridium butyricum in the compound feed, and simplifies the process of feed preparation for farmers.

[0125] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

[0126] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A method for preparing a granular biological feed of Penaeus vannamei fermented with Clostridium butyricum, characterized in that: The following steps are involved: Step 1: Preparation of special formula feed: extruding and granulating the basic feed and the nutritional factors required by the probiotics to obtain pellet feed; Step 2: Aerobic solid fermentation: After activating the aerobic fermentation agent, spray it on the pellet feed, mix it evenly, and place the pellet feed in a constant temperature and humidity room for fermentation; Step 3, anaerobic solid fermentation: the pellet feed that has completed aerobic fermentation in step 2 is collected and placed in an anaerobic mixer, and Clostridium butyricum activation solution is inoculated and fermented in the anaerobic mixer; Step 4: Pack the pellet feed after anaerobic fermentation and store it in sealed plastic bags according to the specifications; The nutritional factors in step 1 include the following raw materials in parts by weight: 8-12 parts of defatted fish meal, 10-20 parts of soybean meal, 10-20 parts of peanut meal, 4-8 parts of flour, 5-10 parts of shrimp shell powder, 3-6 parts of cuttlefish paste, 0.5-1.5 parts of grape seed meal oil, 1-3 parts of sucrose, 0.1-1 parts of calcium dihydrogen phosphate, 0.1-1 parts of potassium dihydrogen phosphate, and 0.5-1.5 parts of potassium dihydrogen sulfate; The expanded particle size in step 1 is 0.5-1.0 mm; The aerobic fermentation agent in step 2 is composed of Bacillus subtilis, Lactobacillus acidophilus, yeast and protease, and the mass ratio of Bacillus subtilis, Lactobacillus acidophilus, yeast and protease is 1-1.2:0.5-0.6:0.1-0.2:0.2-0.3; The aerobic fermentation agent activation method in step 2 comprises the following steps: adding sucrose and 30-50° C. water to a strain expansion tank and stirring evenly, then adding Bacillus subtilis, Lactobacillus acidophilus, yeast, and protease and stirring evenly, and then standing for 60-120 minutes, and finally adding modified nano-silicon dioxide and a loading treatment agent and stirring sufficiently to obtain; The weight of modified nano-silica and loading treatment agent is 5-10% and 1-5% of the total amount of Bacillus subtilis; The modification method of modified nano silicon dioxide is as follows: Adding nano-silicon dioxide to a 5% sodium dodecyl sulfate solution at a weight ratio of 1:5, then adding a chitosan aqueous solution having a mass fraction of 10-15% and a total amount of 2-5% of the nano-silicon dioxide, followed by adding 2-5% of hydroxyacetic acid and 1-5% of dodecylphenol, stirring sufficiently, and finally washing with water and drying to obtain modified nano-silicon dioxide; The loading treatment agent is hydroxyapatite which is calcined at 300-320°C for 10-20min, cooled to 40-45°C, evenly dispersed in 3-5 times the mass fraction of 5% hydrochloric acid solution, and finally washed with water and dried to obtain.

2. The method for preparing a granular biological feed of Penaeus vannamei fermented with Clostridium butyricum according to claim 1, characterized in that: The temperature in step 2 is 30-40° C., and the fermentation time is 24-72 hours.

3. The method for preparing a granular biological feed of Penaeus vannamei fermented with Clostridium butyricum according to claim 1, characterized in that: The fermentation step in step three is: spraying the Clostridium butyricum activation liquid on the surface of the feed placed in the anaerobic mixer after atomization, the anaerobic mixer forms a completely anaerobic environment through a vacuum filtration process, and the product is obtained after standing solid fermentation.

4. The method for preparing a granular biological feed of Penaeus vannamei fermented with Clostridium butyricum according to claim 3, characterized in that: The oxygen partial pressure in the anaerobic mixer is 0.

5. The method for preparing a granular biological feed of Penaeus vannamei fermented with Clostridium butyricum according to claim 3, characterized in that: The fermentation temperature of the static solid fermentation is 35-45°C, and the fermentation time of the static solid fermentation is 48-96h.

6. The method for preparing a granular biological feed of Penaeus vannamei fermented with Clostridium butyricum according to claim 1, characterized in that: In the step 4, the content of Clostridium butyricum in the feed is not less than 1 billion CFU / g.

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