A PRP multilayer separation and extraction test tube

Abstract

The present invention discloses a PRP multi-layer separation and extraction test tube, comprising: a test tube, an upper filter, and an extractor; the upper filter is sleeved on the test tube, the inner wall of the test tube is provided with a straw extending to the middle space, and the extractor is connected to the straw; the bottom of the middle section of the test tube and the bottom of the upper filter are provided with filter screens that can be freely superimposed; the present invention divides the space of the entire test tube into three spaces of upper, middle and lower in a two-layer filter screen mode, and the permeable molecular size of the filter screen is determined by actual application. In actual clinical practice, the upper filter screen ensures that PRP is completely permeable, and the lower filter screen ensures that PRP cannot be permeable, thereby realizing external physical filtration of PRP. Compared with the simple centrifugal form, the substances in the upper and lower layers of PRP will not be excessively integrated into the middle PRP, thereby ensuring the purity of PRP extraction.

Description

Technical Field

[0001] The invention belongs to the technical field of PRP separation, and in particular relates to a PRP multi-layer separation and extraction test tube. Background Art

[0002] PRP stands for Platelet-Rich Plasma (PRP), which is a platelet concentrate extracted from autologous peripheral blood by centrifugation. It contains high concentrations of platelets, white blood cells and fibrin. Platelets can secrete a variety of growth factors after activation, white blood cells can prevent infection, and fibrin can build the three-dimensional structure required for tissue repair locally. The concentrated PRP component not only provides "concentrated nutrition" for tissue repair, but also builds a better repair environment for tissue repair, which can promote and accelerate the repair of bone tissue and soft tissue.

[0003] Moreover, all prepared PRPs are in liquid form. Liquid PRP is mainly used for joint cavity or local injection. Liquid PRP can form gel-like PRP after being activated by thrombin or calcium. The gel can cover the wound surface or fill the tissue that needs to be repaired.

[0004] The biggest problem with PRP treatment is the separation and extraction of PRP. Blood is collected in blood collection tubes in most clinics now, and then the blood in the tubes is centrifuged. PRP is distributed in the middle layer. After complete precipitation, a syringe is directly inserted into the middle layer to extract PRP. However, in this process, whether it is due to human factors or incomplete centrifugal separation, the extracted PRP will eventually be of insufficient purity or mixed with other substances, affecting subsequent disease treatment. High-purity separation and extraction has become a major problem that needs to be overcome.

[0005] In order to overcome the above problems, various structures that help purification and separation are designed in the prior art, such as the Chinese patent with the authorization publication number CN114405069A, which discloses a PRP extraction device, which extracts PRP through an extraction tube, without the need to insert a long needle tube for extraction, reducing the interference of human factors, and the extracted PRP has a higher purity and is more stable. At the same time, the end cover seals the connector to ensure the stability of the extraction tube during the centrifugation process. However, this prior art only improves the extraction environment of PRP. In actual use, it only avoids the long needle insertion and absorption process, and does not solve the problem of PRP extraction purity from the actual centrifugal stratification.

[0006] Therefore, there is a need for a separation and extraction device that can solve the purification problem essentially from the centrifugal stratification and avoid the insertion of a long needle for aspiration, or an extraction and separation tube combined with centrifugation. Summary of the invention

[0007] In order to solve the above technical problems, the present invention provides a PRP multi-layer separation and extraction test tube, in which a fixed extraction pipe is arranged in the test tube to avoid contamination from the upper clear liquid when extracting PRP; at the same time, the test tube is provided with a multi-layer filter screen that can be freely combined to ensure that each layer of material is accurately separated during the centrifugation process.

[0008] In order to achieve the above technical effects, the present invention is implemented through the following technical solutions: a PRP multi-layer separation and extraction test tube, comprising: a test tube, an upper filter, and an extractor;

[0009] The upper filter is sleeved on the test tube, the inner wall of the test tube is provided with a straw extending to the middle space, and the extractor is connected to the straw;

[0010] The bottom of the middle section of the test tube and the bottom of the upper filter are provided with filter screens that can be stacked freely;

[0011] Furthermore, the middle section of the test tube and the bottom of the upper filter are fixedly provided with a base filter that completely covers the cross section thereof, and the base filter is overlapped with an adjustment filter; the adjustment filter is completely in contact with the base filter; the upper filter is sleeved above the middle section of the test tube;

[0012] Furthermore, the base filter screen has a protrusion on its periphery, and the adjustment filter screen is clipped onto the protrusion of the base filter screen;

[0013] Furthermore, the outer periphery of the adjusting filter is provided with a protrusion for clamping the upper adjusting filter;

[0014] Furthermore, a pull bar is fixedly provided on the outer periphery of the adjusting filter screen, and a limit block is fixedly provided on the top of the pull bar, and the pull bar is hung on the side wall of the test tube or the side wall of the upper filter through the limit block;

[0015] Furthermore, on the inner wall of the test tube and the side wall of the upper filter, reeds distributed along the side wall are fixedly provided, and the pull bar of the adjustment filter is clamped on the inner side of the reed;

[0016] Furthermore, the extractor comprises a main body conduit for extraction, a squeezing balloon is fixedly provided on the main body conduit, and one-way valves for flowing to the outside are fixedly provided at both ends of the squeezing balloon.

[0017] The beneficial effects of the present invention are:

[0018] Firstly, the present invention divides the space of the entire test tube into three spaces, namely, upper, middle and lower spaces, in a two-layer filter mode, and the molecular size of the permeable filter is determined by actual application. In actual clinical practice, the upper filter ensures that PRP is completely permeable, while the lower filter ensures that PRP cannot pass through, thereby realizing external physical filtration of PRP. Compared with simple centrifugation, the substances in the upper and lower layers of PRP will not be excessively integrated into the PRP in the middle, thereby ensuring the purity of PRP extraction.

[0019] Secondly, the device adds an adjustable filter in the form of filter filtration and centrifugation. In actual use, the molecular pass rate standards are set after different filters are snapped together. According to the size of the molecules to be filtered, the filters can be freely closed, so that the size of the filtered molecules can be adjusted to achieve precise filtration.

[0020] Another difference from the prior art is that in the present invention, a straw extending to the middle section is provided on the side wall of the test tube, and the tip of the straw is actually configured to just reach the middle PRP layer, which is convenient for direct PRP and avoids cross contamination caused by inserting a long needle from the supernatant, thereby improving the purity of PRP extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for describing the embodiments are briefly introduced below.

[0022] Figure 1 It is a schematic diagram of the overall structure of a PRP multi-layer separation and extraction test tube;

[0023] Figure 2 It is a schematic diagram of the internal structure of a PRP multi-layer separation and extraction test tube;

[0024] Figure 3 It is a schematic diagram of the internal structure of the upper filter of a PRP multi-layer separation and extraction test tube;

[0025] Figure 4 It is a schematic diagram of a test tube and its accessory structure of a PRP multi-layer separation and extraction test tube;

[0026] Figure 5 It is a schematic diagram of the upper filter and its attached structure of a PRP multi-layer separation and extraction test tube;

[0027] Figure 6 The present invention is a schematic diagram of an extractor of a PRP multi-layer separation and extraction test tube and its accessory structure.

[0028] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0029] 1-test tube, 11-reed, 12-base filter, 13-adjusting filter, 14-pull rod, 15-straw, 16-limiting block, 2-upper filter, 21-column, 211-hook, 3-extractor, 31-main catheter, 32-extrusion balloon, 33-check valve. DETAILED DESCRIPTION

[0030] The present invention provides a PRP multi-layer separation and extraction test tube, comprising: a test tube 1, an upper filter 2, and an extractor 3;

[0031] The upper filter 2 is sleeved on the test tube 1, and a straw 15 extending to the middle space is provided on the inner wall of the test tube 1, and the extractor 3 is connected to the straw 15. Through the cooperation of the extractor 3 and the straw 15, the PRP in the middle part can be directly sucked, avoiding the problem of the suction pipeline intervening from the upper clear liquid. Compared with the traditional suction form, there is no cross-contamination problem, which improves the purity of PRP extraction; the bottom of the middle section of the test tube 1 and the bottom of the upper filter 2 are provided with freely stackable filter screens, and the extraction purity of PRP is improved by constructing a basic layered filter screen, so that the substances in the upper and lower layers will not excessively escape into the middle PRP, avoiding pollution of PRP; at the same time, the present invention adopts the form of superimposed filter screens to change the molecular diameter size that the filter screen can pass through, further control the entry of impurities into the PRP, and ensure its separation purity.

[0032] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention; the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0033] Example 1

[0034] Based on the above, this embodiment provides a preferred embodiment of the filter arrangement of the PRP multi-layer separation and extraction test tube 1;

[0035] In this embodiment, a base filter 12 is fixedly arranged at the middle section of the test tube 1 and the bottom of the upper filter 2 to completely cover the cross section thereof, and an adjustment filter 13 is overlapped on the base filter 12, and the adjustment filter 13 is in full contact with the base filter 12; the two base filters 12 constitute a space for the existence of the middle PRP, and the upper and lower layers of substances are isolated in the form of a filter during the centrifugation process. Of course, in this embodiment, the upper base filter 12 allows the PRP to pass through, but the lower base filter 12 does not allow the PRP to pass through;

[0036] Secondly, in the present embodiment, the upper filter 2 is sleeved on the middle section of the test tube 1; this is the basic condition for the normal operation of the upper filter 2. The filter molecular size of the upper filter 2 is adjusted by superimposing an adjustment filter 13 of specified specifications on the base filter 12; the adjustment of the filter molecular size of the lower base filter 12 is achieved by repeatedly superimposing the adjustment filter 13 on the base filter 12.

[0037] Example 2

[0038] Based on the above-mentioned embodiment 1, this embodiment provides a preferred implementation method in which the base filter 12 and the adjustment filter 13 overlap and lock with each other;

[0039] In this embodiment, a protrusion is provided on the outer periphery of the base filter 12, and the adjusting filter 13 is snapped into the raised inner circle of the base filter 12; as for the adjustment of the filter filtration, in this embodiment, a protrusion for snapping into the upper adjusting filter 13 is also provided on the outer periphery of the adjusting filter 13, that is, the adjusting filter 13 is added when necessary to reduce the size of the molecules passing through the filtration.

[0040] Example 3

[0041] Based on the above-mentioned embodiment 2, in order to solve the problem of convenient replacement and stacking of the adjustable filter 13, in this embodiment, a pull strip 14 is fixedly provided on the outer periphery of the adjustable filter 13, and a limit block 16 is also fixedly provided on the top of the pull strip 14, which is hung on the side wall of the test tube 1 or the side wall of the upper filter 2 through the limit block 16; in actual use, the pull strip 14 can be directly held to realize the operation of the adjustable filter 13, including overlapping and clamping it together, or taking out the adjustable filter 13 for replacement.

[0042] Example 4

[0043] Based on the above-mentioned embodiment 3, in order to improve the stability of the adjusting filter 13 during the centrifugation process; in this embodiment, on the inner wall of the test tube 1 and the side wall of the upper filter 2, a spring sheet 11 distributed along the side wall is fixed, and the pull rod 14 of the adjusting filter 13 is clamped on the inner side of the reed sheet 11, and the pull rod 14 is tightened to the inner wall of the test tube 1 or the inner wall of the upper filter 2 through the spring sheet 11 to avoid the adjusting filter 13 being dislocated due to the gravity of the pull rod 14 during the centrifugation process.

[0044] Example 5

[0045] In order to realize the active extraction of PRP in vitro, this embodiment provides a preferred form of an extractor 3 to suck the PRP in the middle layer from the suction tube 15;

[0046] The extractor 3 of this embodiment includes a main catheter 31 for extraction, on which a squeezing balloon 32 is fixedly provided, and both ends of the squeezing balloon 32 are fixedly provided with a one-way valve 33 for outward flow. When in use, the main catheter 31 is firstly buckled onto the straw 15 to compress the squeezing balloon 32 to create a negative pressure environment inside the straw 15. Under the action of the one-way valve 33, the squeezing balloon 32 can only absorb PRP from one direction of the straw 15 but cannot absorb air from the outside, thereby ensuring the stable extraction of PRP.

[0047] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to only the specific implementation methods described.

Claims

1. A PRP multilayer separation and extraction test tube, characterized in that: include: Test tubes, upper filters, extractors; The upper filter is sleeved on the test tube, the inner wall of the test tube is provided with a straw extending to the middle space, and the extractor is connected to the straw; The bottom of the middle section of the test tube and the bottom of the upper filter are provided with filter screens that can be stacked freely; The middle section of the test tube and the bottom of the upper filter are fixed with a base filter that completely covers the cross section, and the base filter is overlapped and engaged with an adjustment filter; the adjustment filter is in full contact with the base filter; the upper filter is sleeved above the middle section of the test tube; A pull bar is fixedly provided on the outer periphery of the adjusting filter screen, and a limit block is fixedly provided on the top of the pull bar, which is hung on the side wall of the test tube or the side wall of the upper filter through the limit block; On the inner wall of the test tube and the side wall of the upper filter, reeds distributed along the side wall are fixedly provided, and the pull strips of the adjusting filter screen are clamped on the inner side of the reeds.

2. A PRP multilayer separation and extraction test tube according to claim 1, characterized in that: The outer periphery of the base filter is provided with a protrusion, and the regulating filter is clamped on the protrusion of the base filter.

3. A PRP multilayer separation and extraction test tube according to claim 1, characterized in that: The outer periphery of the regulating filter is provided with a protrusion for clamping the upper regulating filter.

4. A PRP multilayer separation and extraction test tube according to claim 1, characterized in that: The extractor comprises a main body conduit for extraction, a squeezing balloon is fixedly arranged on the main body conduit, and one-way valves for flowing outward are fixedly arranged at both ends of the squeezing balloon.

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