A method of mango seed storage

Abstract

This invention provides a method for storing mango seeds, belonging to the field of seed preservation technology. The method involves cutting off mango fruits with embryos, along with their pedicels and branches, 77-84 days after fruit set. A mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is sprayed on the fruit, pedicels, and branches. After drying, styrene-butadiene latex is applied to the pedicels and branches. Once the latex dries, a mixture of guanidine-copper acetate and benzoyl-pyraclostrobin is sprayed on the fruit, pedicels, and branches again. After drying, the mango fruits with petioles and branches are placed in a sealed box and stored at 10-14°C. The mango variety used is the Golden Phoenix mango. This invention increases the storage period of Golden Phoenix mango seeds from 1.5 months to 5 months, which is of great significance for the genomics and cell biology research of Golden Phoenix mangoes.

Description

Technical Field

[0001] This invention belongs to the field of seed preservation technology, and in particular relates to a method for storing mango seeds. Background Technology

[0002] Mango is an important tropical fruit tree, and its seeds are notoriously difficult to store. While most angiosperm seeds can remain viable for years or even decades under dry, cool, and natural conditions after harvesting, mango seeds quickly lose their viability when stored naturally. Our previous research found that mature Golden Phoenix mango seeds, when stored naturally, exhibited a significantly reduced germination rate after 5 days, only about half after 7 days, and nearly zero germination after 9 days, essentially losing their viability.

[0003] Golden Phoenix mango is one of the representative varieties of mangoes produced in Yunnan Province. Its fruit is of moderate size, beautiful shape, and vibrant color. It is characterized by low fiber content, no pronounced pine aroma, and abundant juice, making it very popular with local consumers. Fruit farmers also cultivate Golden Phoenix as their primary variety. Due to the superior economic traits and large planting area of ​​Golden Phoenix mangoes, many agricultural researchers have conducted systematic studies on it in recent years, especially in genomics and cell biology. This research requires a large number of viable, readily germinating Golden Phoenix mango seeds for seedling cultivation, and the collection of samples from young leaves, tender stems, and root tip meristems for further analysis.

[0004] Currently, for seeds of commonly used rootstock varieties such as Tainong and Xiaoji mango, producers generally adopt the method of sowing immediately after harvesting, rarely storing them. Studies have found that storing mango seeds using methods such as moist coconut coir or moist fine sand can maintain seed viability for about 3 months. However, due to the varietal characteristics of the Golden Phoenix mango, the publicly reported methods such as using moist coconut coir or moist fine sand can only maintain seed viability for about 1.5 months, which is far from meeting the requirements of scientific research.

[0005] For the reasons mentioned above, developing a method for storing Golden Phoenix mango seeds is of great significance for ensuring the sampling of seedlings for scientific research and promoting systematic research on the genomics and cell biology of Golden Phoenix mango. Summary of the Invention

[0006] In view of this, the purpose of the present invention is to provide a method for storing mango seeds, which can effectively extend the storage period of Golden Phoenix mango seeds.

[0007] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0008] A method for storing mango seeds includes the following steps: 77-84 days after mango fruit set, cut off the mango fruit with embryo along with the fruit stalk and branch, spray with a mixture of pyraclostrobin + high-efficiency cyhalothrin + chlorothalonil, let it dry, then apply styrene-butadiene latex to the surface of the fruit stalk and branch, after the latex dries, spray the surface of the fruit, fruit stalk and branch again with a mixture of guanidine·copper acetate + benzoyl·pyraclostrobin, and after drying, place the mango fruit with petiole and branch in a sealed box and store it at 10-14℃.

[0009] The mango in question is the Golden Phoenix mango.

[0010] Preferably, the mixture of pyraclostrobin + lambda-cyhalothrin + chlorothalonil is prepared as follows: pyraclostrobin is a suspension with 30% active ingredient diluted 300-400 times with water; lambda-cyhalothrin is a microcapsule suspension with 10% active ingredient diluted 150-200 times with water; and chlorothalonil is a suspension with 40% active ingredient diluted 100-150 times with water.

[0011] Preferably, the mixture of guanidine·copper acetate + benzoyl·pyrazole ester is prepared as follows: guanidine·copper acetate is a wettable powder with 20% active ingredient, diluted 50-80 times with water; benzoyl·pyrazole ester is a suspension with 32% active ingredient, diluted 200-300 times with water.

[0012] Preferably, the styrene-butadiene latex has a solid content of 50% and a viscosity of 1300-2000 mPa·s.

[0013] Preferably, the main body of the sealed box is divided into an upper storage space and a lower water-proof space by a perforated partition. In the storage space, reduced iron powder is placed on the partition, then a gauze layer is laid on top, and then mango fruits with petioles and branches are placed on it. The fruits are covered with a gauze layer, and then coconut shell activated carbon is placed on the gauze layer.

[0014] Preferably, 10-15g of chlorine dioxide effervescent tablets are placed in the water-proof space at the bottom of the sealed box.

[0015] Preferably, the sealed box is a foam sealed box, with foam blocks placed at the four corners of the water-proof space, and the partition is a foam board placed on the foam blocks. The foam board has 4-9 holes with a hole diameter of 1-1.5cm.

[0016] Preferably, the reduced iron powder and coconut shell activated carbon in the sealed box should be replaced once a month, and the moisture under the partition should be removed.

[0017] Preferably, when the Golden Phoenix mango is in bloom, cut off the top 2 / 3 of the inflorescence, leaving the small flowers at the base of the inflorescence that bloom at the same time, and hang the tags during the peak flowering period. After flowering and fruit setting, manage it as usual, and remove the non-embryo fruits 40-45 days after fruit setting.

[0018] Preferably, after the mango fruit is cut off along with the stem and branch, the top of the fruit is placed upwards and left for 1-1.5 hours until the secretion at the cut stop, then a mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is sprayed.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] This invention provides a method for storing mango seeds, which stores Golden Phoenix mango seeds with the fruit attached, increasing the storage period of Golden Phoenix mango seeds from 1.5 months to about 5 months. This is of great significance for ensuring sampling for scientific seedling cultivation and promoting genomics and cell biology research on Golden Phoenix mangoes. The facilities and equipment used in this invention are common, the operation steps are simple, and it is easy to use. Detailed Implementation

[0021] This invention provides a method for storing mango seeds, comprising the following steps: 77-84 days after fruit set, mango fruits with embryos are cut off along with their stems and branches. A mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is sprayed on the fruit, stems, and branches. After drying, styrene-butadiene latex is applied to the surface of the stems and branches. After the latex dries, a mixture of guanidine-copper acetate and benzoyl-pyraclostrobin is sprayed on the surface of the fruit, stems, and branches again. After drying, the mango fruits with stems and branches are placed in a sealed box and stored at 10-14℃. The mango is a Golden Phoenix mango.

[0022] Golden Phoenix mango seeds are notoriously inactive; mature seeds quickly lose their viability after just nine days of storage under natural conditions. This invention addresses this issue by storing the mango seeds with the fruit intact. The mango fruit is cut off along with its stem and branch, preventing moisture loss and extending its shelf life. The seeds are encased in the fruit pulp, mimicking the seed's natural growth environment within the fruit, thus preventing rapid moisture loss and maintaining seed viability in a near-dormant state. Furthermore, by controlling the harvesting time, germination is inhibited during storage, effectively extending the storage time of the Golden Phoenix mango seeds.

[0023] In this invention, the top two-thirds of the inflorescence of the Golden Phoenix mango is cut off when it blooms, leaving the small flowers at the base of the inflorescence that bloom at the same time. The inflorescence is then tagged during peak bloom. After flowering and fruit set, routine management is performed. 40-45 days after fruit set, fruits without embryos are removed. This invention promptly removes excess inflorescences and fruits without embryos, improving the nutrient utilization rate of the mango tree, increasing the fruit set rate, and ensuring that all harvested mangoes contain highly active seeds.

[0024] In this invention, mangoes with embryos are cut off along with their pedicels and branches 77-84 days after fruit set. Preferably, the mangoes with embryos are cut off along with their pedicels and branches 79-82 days after fruit set, with the branches retained to a length of 20-25 cm. The research in this invention has found that mango seeds are prone to visible germination in mature fruit, and the length of the mango's growth period affects the seed's dehydration sensitivity, thus significantly impacting the storage time of mango seeds. The method provided by this invention, by controlling the harvesting time of mangoes, ensures that the mango seeds accumulate sufficient nutrients and have high activity, while preventing germination during subsequent storage, thereby extending the storage time of mango seeds.

[0025] In this invention, after the mango fruit is cut off along with the stem and branch, the top of the fruit is placed upwards and left for 1-1.5 hours until the secretion at the cut stop, a mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is sprayed.

[0026] In this invention, after the mango fruit, along with its stem and branch, is cut off, the leaves on the stem and branch are removed. The fruit is then placed on a wire mesh with the stem and branch passing through the mesh downwards and the fruit tip upwards. The wire mesh has a mesh diameter of 2-4 cm. If the mesh is too small, the stem and branch will not easily pass through; if the mesh is too large, the fruit may get stuck or even fall off. After 1-1.5 hours, once the secretions at the cut have stopped, a mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is sprayed using a small sprayer until the surface of the fruit, stem, and branch is completely moistened and begins to drip. A second spray is applied after half an hour.

[0027] In this invention, the mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is prepared as follows: pyraclostrobin (30% active ingredient) is diluted 300-400 times with water; lambda-cyhalothrin (10% active ingredient) is diluted 150-200 times with water; and chlorothalonil (40% active ingredient) is diluted 100-150 times with water. Preferably, the mixture is prepared as follows: pyraclostrobin (30% active ingredient) is diluted 340-360 times with water; lambda-cyhalothrin (10% active ingredient) is diluted 170-190 times with water; and chlorothalonil (40% active ingredient) is diluted 120-130 times with water. In this invention, the concentrations of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil are the concentrations in the mixed pyraclostrobin + lambda-cyhalothrin + chlorothalonil mixture.

[0028] Mangoes are typical tropical fruits, highly susceptible to infection by various pathogens and microorganisms, and prone to spoilage and decay due to their poor storage properties. This invention, through spraying a mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil, effectively inhibits the infection of mangoes by pathogens and microorganisms, suppresses the hatching of fruit fly eggs beneath the mango peel, prevents fruit rot, extends the storage time of mangoes, and provides a favorable environment for the preservation of mango seeds.

[0029] In this invention, the styrene-butadiene latex has a solid content of 50% and a viscosity of 1300-2000 mPa·s, preferably 1500-1800 mPa·s. This invention uses styrene-butadiene latex to coat the surface of the mango fruit, including the stem and branches, forming an insulating layer that prevents moisture loss and extends the storage time of the mango seeds.

[0030] As one feasible method, this invention uses a brush to apply styrene-butadiene latex evenly to the surface of the fruit, its pedicel, and branches, avoiding brushing the fruit itself. After the styrene-butadiene latex on the pedicel and branches dries, a mixture of guanidine-copper acetate and benzoyl-pyrazole ester is sprayed onto the fruit, pedicel, and branches until the surface is completely wetted and begins to drip. The fruit, pedicel, and branches are then allowed to air dry in the shade until they are essentially dry.

[0031] In this invention, the guanidine-copper acetate + benzyl-pyrazole ester mixture is prepared as follows: a wettable powder containing 20% ​​guanidine-copper acetate as the active ingredient is diluted 50-80 times with water; a suspension containing 32% benzoyl-pyrazole ester as the active ingredient is diluted 200-300 times with water. Preferably, a wettable powder containing 20% ​​guanidine-copper acetate as the active ingredient is diluted 60-70 times with water; and a suspension containing 32% benzoyl-pyrazole ester as the active ingredient is diluted 230-260 times with water. In this invention, the concentrations of guanidine-copper acetate and benzyl-pyrazole ester are defined as their concentrations in the mixed guanidine-copper acetate + benzyl-pyrazole ester mixture.

[0032] Copper acetate and guanidine can kill viruses attached to the surface of mangoes, while benzyl benzoate and pyrazole ester can kill bacteria and fungi on the surface of mangoes. This invention, by spraying a mixture of copper acetate and benzyl benzoate and pyrazole ester, can inhibit viral and microbial infection of mangoes and prevent fruit rot.

[0033] In this invention, the treated mango fruits with petioles and branches are placed in a sealed box and refrigerated at 10-14℃. Preferably, the mango fruits with petioles and branches are placed in a sealed box and refrigerated at 12-13℃. The research conducted in this invention found that excessively high or low storage temperatures of mango fruits can lead to rapid seed deactivation. The storage temperature provided by this invention allows Golden Phoenix mango seeds to be stored for approximately 5 months.

[0034] In this invention, the main body of the sealed box is divided into an upper storage space and a lower water-proof space by a perforated partition. In the storage space, reduced iron powder is placed on the partition, then a gauze layer is laid on top, and then mango fruits with petioles and branches are placed on top. The fruits are covered with a gauze layer, and then coconut shell activated carbon is placed on the gauze layer.

[0035] The sealed box described in this invention is sterilized before use. As one feasible method, this invention involves spraying the foam box twice with 75% alcohol evenly, with an interval of 15 minutes.

[0036] This invention involves placing 20-30g of reduced iron powder wrapped in a microporous nylon membrane on a partition, then covering it with a 0.3-0.5cm thick layer of gauze. Mango fruits with petioles and branches are then placed on top of the gauze, separated by sterile paper. No more than three layers of fruits are arranged. The fruits are then covered with another 0.3-0.5cm thick layer of gauze, and finally, 30-50g of coconut shell activated carbon wrapped in a microporous nylon membrane is placed on top of the gauze. As one feasible implementation, the microporous nylon membrane used in this invention has a pore size of 10-15μm.

[0037] In this invention, 10-15g of chlorine dioxide effervescent tablets are placed in the water-proof space at the bottom of the sealed box.

[0038] In this invention, the sealed box is a foam sealed box, with a length, width, and height of 50cm and a thickness of 2.5cm. Foam blocks are placed at the four corners of the water-proof space, and the partition is a foam board placed on the foam blocks. The foam board has 4-9 holes with a hole diameter of 1-1.5cm. In this invention, the foam blocks placed at the four corners of the water-proof space are square foam blocks with a diameter of 1-2cm. This invention uses a perforated partition to divide the sealed box into upper and lower layers, increasing air permeability and allowing the moisture lost from the mango fruit to accumulate in the lower water-proof layer, preventing the mango from directly contacting water and causing it to rot and decay.

[0039] In this invention, the reduced iron powder and coconut shell activated carbon in the sealed box are replaced once a month, and the moisture under the partition is removed. By placing the reduced iron powder under the mango fruit, this invention can effectively absorb moisture, oxygen, and other waste gases, and the storage condition of the mango can be determined by observing the state of the reduced iron powder.

[0040] In this invention, before sowing mango seeds, branches and pulp are removed, and the seed coat is peeled off for conventional sowing.

[0041] Unless otherwise specified, the reagents, consumables, etc. involved in this invention are all commercially available. Unless otherwise specified, they are generally used under conventional conditions or as recommended by the company.

[0042] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0043] Example 1

[0044] When the Golden Phoenix mango is in bloom, cut off the top 2 / 3 of the inflorescence, leaving the small flowers at the base of the inflorescence that bloom at the same time. Then, hang a tag during the peak flowering period and manage it as usual after flowering and fruit setting.

[0045] Remove the non-embryo fruits 40 days after fruit set, and cut off the embryo fruits along with the fruit stalks and branches 77 days after fruit set, keeping the branches 25cm long.

[0046] Remove the leaves from the fruit stalks and branches. Place the fruit on a wire mesh with the stalks and branches passing through the mesh downwards and the fruit tops upwards. Leave it for 1.5 hours until the secretions from the cut stop. Then, use a small sprayer to spray a mixture of pyraclostrobin (30% active ingredient suspension diluted 300 times with water), lambda-cyhalothrin (10% active ingredient microcapsule suspension diluted 150 times with water), and chlorothalonil (40% active ingredient suspension diluted 100 times with water) (the concentration of each component is the final concentration after mixing). Spray evenly until the surface of the fruit, stalks, and branches is completely wet and begins to drip. Spray again after half an hour.

[0047] Dip a brush into styrene-butadiene latex (50% solids content, 1500 mPa·s viscosity) and apply it evenly to the surface of the fruit, stem, and branches, but do not brush the fruit. After the styrene-butadiene latex on the stem and branches is dry, spray the fruit, stem, and branches with a mixture of guanidine copper acetate (20% active ingredient wettable powder diluted 50 times with water) and benzyl pyrazole ester (32% active ingredient suspension diluted 200 times with water) (the concentration of each component is the final concentration after mixing). Spray evenly until the surface of the fruit, stem, and branches is completely wet and begins to drip water. Let it air dry in the shade until the fruit, stem, and branches are basically dry.

[0048] Prepare a foam sealing box with dimensions of 50cm x ...

[0049] Place the sealed box in a refrigerator at 14°C. Replace the reduced iron powder and coconut shell activated carbon inside the sealed box once a month, and remove the moisture from under the foam board inside the sealed box.

[0050] After 5 months of storage, the fruit was removed, branches and pulp were removed, the seed coat was peeled off, and conventional sowing was carried out. Based on the germination rate of the Golden Phoenix mango seeds after sowing, the seed viability was determined to be 100%, which can meet the requirements of scientific research seedling cultivation and sampling.

[0051] Example 2

[0052] When the Golden Phoenix mango is in bloom, cut off the top 2 / 3 of the inflorescence, leaving the small flowers at the base of the inflorescence that bloom at the same time. Then, hang a tag during the peak flowering period and manage it as usual after flowering and fruit setting.

[0053] Remove the non-embryo fruits 45 days after fruit set, and cut off the embryo fruits along with the fruit stalks and branches 84 days after fruit set, keeping the branches 20cm long.

[0054] Remove the leaves from the fruit stalks and branches. Place the fruit on a wire mesh with the stalks and branches passing through the mesh downwards and the fruit tops upwards. Leave it for 1.2 hours until the secretions from the cut stop. Then, use a small sprayer to spray a mixture of pyraclostrobin (30% active ingredient suspension diluted 400 times with water), lambda-cyhalothrin (10% active ingredient microcapsule suspension diluted 200 times with water), and chlorothalonil (40% active ingredient suspension diluted 150 times with water) (the concentrations of each component are the final mixed concentrations). Spray evenly until the surface of the fruit, stalks, and branches is completely wet and begins to drip. Spray again after half an hour.

[0055] Dip a brush into styrene-butadiene latex (50% solids content, 2000 mPa·s viscosity) and apply it evenly to the surface of the fruit, stem, and branches, but do not brush the fruit. After the styrene-butadiene latex on the stem and branches is dry, spray the fruit, stem, and branches with a mixture of guanidine copper acetate (20% active ingredient wettable powder diluted 80 times with water) and benzyl pyrazole ester (32% active ingredient suspension diluted 300 times with water) (the concentration of each component is the final concentration after mixing). Spray evenly until the surface of the fruit, stem, and branches is completely wet and begins to drip water. Let it air dry in the shade until the fruit, stem, and branches are basically dry.

[0056] Prepare a foam sealing box with dimensions of 50cm x ...

[0057] Place the sealed box in a refrigerator at 13°C. Replace the reduced iron powder and coconut shell activated carbon inside the sealed box once a month, and remove the moisture from under the foam board inside the sealed box.

[0058] After 5 months of storage, the fruit was removed, branches and pulp were removed, the seed coat was peeled off, and conventional sowing was carried out. Based on the germination rate of the Golden Phoenix mango seeds after sowing, the seed viability was determined to be 95%, which can meet the requirements for scientific research seedling cultivation and sampling.

[0059] Example 3

[0060] When the Golden Phoenix mango is in bloom, cut off the top 2 / 3 of the inflorescence, leaving the small flowers at the base of the inflorescence that bloom at the same time. Then, hang a tag during the peak flowering period and manage it as usual after flowering and fruit setting.

[0061] Remove the non-embryo fruits 42 days after fruit set, and cut off the embryo fruits along with the fruit stalks and branches 80 days after fruit set, keeping the branches 22cm in length.

[0062] Remove the leaves from the fruit stalks and branches. Place the fruit on a wire mesh with the stalks and branches passing through the mesh downwards and the fruit tops upwards. Leave it for 1.3 hours until the secretions from the cut stop. Then, use a small sprayer to spray a mixture of pyraclostrobin (30% active ingredient suspension diluted 350 times with water), lambda-cyhalothrin (10% active ingredient microcapsule suspension diluted 180 times with water), and chlorothalonil (40% active ingredient suspension diluted 120 times with water). Spray evenly until the surface of the fruit, stalks, and branches is completely wet and begins to drip. Spray again after half an hour.

[0063] Dip a brush into styrene-butadiene latex (50% solids content, 1800 mPa·s viscosity) and apply it evenly to the surface of the fruit, stem, and branches, but do not brush the fruit. After the styrene-butadiene latex on the stem and branches is dry, spray the fruit, stem, and branches with a mixture of guanidine copper acetate (20% active ingredient wettable powder diluted 60 times with water) and benzyl pyrazole ester (32% active ingredient suspension diluted 250 times with water) (the concentration of each component is the final concentration after mixing). Spray evenly until the surface of the fruit, stem, and branches is completely wet and begins to drip water. Let it air dry in the shade until the fruit, stem, and branches are basically dry.

[0064] Prepare a foam sealing box with dimensions of 50cm x ...

[0065] Place the sealed box in a refrigerator at 12°C. Replace the reduced iron powder and coconut shell activated carbon inside the sealed box once a month, and remove the moisture from under the foam board inside the sealed box.

[0066] After 5 months of storage, the fruit was removed, branches and pulp were removed, the seed coat was peeled off, and conventional sowing was carried out. Based on the germination rate of the Golden Phoenix mango seeds after sowing, the seed viability was still 98%, which can meet the requirements of scientific research seedling cultivation and sampling.

[0067] Comparative Example 1

[0068] When the Golden Phoenix mango is in bloom, cut off the top 2 / 3 of the inflorescence, leaving the small flowers at the base of the inflorescence that bloom at the same time. Then, hang a tag during the peak flowering period and manage it as usual after flowering and fruit setting.

[0069] Remove the non-embryo fruits 40 days after fruit set, and cut off the embryo fruits along with the fruit stalks and branches 77 days after fruit set, keeping the branches 25cm long.

[0070] Remove the pulp from the obtained mango fruit and store the fresh seeds in a foam box filled with moist coconut coir and granular activated carbon (the volume ratio of moist coconut coir to granular activated carbon is 1:1, and then mix the two together). Place the foam box in a refrigerator at 14°C for cold storage.

[0071] In this comparative example, the mango pulp was removed, and the seeds were stored directly. Compared to Example 1, the seeds lost moisture more quickly. After 2 months of storage, the seed viability was 45%, after 3 months it was 15%, and after 5 months it was 0%. It is evident that the method provided by this invention significantly extends the storage time of Golden Phoenix mango seeds.

[0072] Comparative Example 2

[0073] The difference between this comparative example and Example 1 is that the non-embryo fruits were removed 40 days after fruit set, and the embryo fruits were cut off along with the fruit stalks and branches 91 days after fruit set, leaving only the fruit stalks and not the branches.

[0074] After 5 months of storage, the seed viability was 88%. In this comparative example, the mango fruit growth period was longer than in Example 1, and the mango seed germination was more pronounced. Furthermore, due to the absence of branches, there was greater sap loss and water loss at the fruit stalk, which promoted fruit ripening and resulted in a partial decrease in seed viability.

[0075] Comparative Example 3

[0076] The difference between this comparative example and Example 1 is that the non-embryo fruits were removed 40 days after fruit set, and the embryo fruits were cut off along with the fruit stalks and branches 105 days after fruit set, retaining only the fruit stalks and not the branches.

[0077] After 5 months of storage, the seed viability was 75%. In this comparative example, the mango fruit was harvested 105 days after fruit set, when the seed germination rate was further enhanced. During storage, the seed metabolism was vigorous and respiration was increased, leading to a decrease in seed viability after 5 months of storage.

[0078] Comparative Example 4

[0079] The difference between this comparative example and Example 1 is that the mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil was not sprayed.

[0080] After 5 months of storage, the seed viability was 45%. During the storage process, hatched fruit flies were observed, causing some fruits and seeds to rot, and the viability of the remaining seeds also decreased significantly.

[0081] Comparative Example 5

[0082] The difference between this comparative example and Example 1 is that the phrase "spraying a mixture of pyraclostrobin (30% active ingredient suspension diluted 300 times with water) + lambda-cyhalothrin (10% active ingredient microcapsule suspension diluted 150 times with water) + chlorothalonil (40% active ingredient suspension diluted 100 times with water)" in Example 1 is changed to "spraying a mixture of pyraclostrobin (50% active ingredient dispersant diluted 500 times with water) + trichlorfon (90% active ingredient soluble powder diluted 500 times with water) + thiophanate-methyl (80% active ingredient wettable powder diluted 300 times with water)" in Example 1 using a small sprayer.

[0083] After 5 months of storage, the seed viability was 82%, which is not as good as the seed preservation effect in Example 1.

[0084] Comparative Example 6

[0085] The difference between this comparative example and Example 1 is that styrene-butadiene latex was not applied to the surface of the fruit stalk and branches, nor was a mixture of guanidine-copper acetate + benzoyl-pyrazole ester sprayed onto the surface of the fruit, fruit stalk and branches.

[0086] After 5 months of storage, the seed viability was 50%. During the storage process, the fruit lost moisture rapidly, resulting in wilting of the mango fruit, accompanied by microbial infection, which led to the rotting of some fruit and seeds, and a decrease in seed viability.

[0087] Comparative Example 7

[0088] The difference between this comparative example and Example 1 is that the foam board used as a partition in the sealed box was not perforated.

[0089] After 5 months of storage, the seed viability was 72%. During the storage process, obvious water accumulation appeared at the bottom of the mango fruit, and a few fruits and seeds rotted, resulting in a decrease in mango seed viability.

[0090] Comparative Example 8

[0091] The difference between this comparative example and Example 1 is that 15g of chlorine dioxide effervescent tablets were not placed in the middle of the bottom of the sealed box.

[0092] After 5 months of storage, the seed viability was 86%.

[0093] Comparative Example 9

[0094] The difference between this comparative example and Example 1 is that the operation of "replacing the reduced iron powder and coconut shell activated carbon in the sealed box once a month and removing the moisture under the foam board in the sealed box" was not performed.

[0095] After 5 months of storage, the seed viability was 40%. During the storage process, the high moisture content inside the sealed box caused some fruits and seeds to rot.

[0096] Comparative Example 10

[0097] The difference between this comparative example and Example 1 is that it was stored at 18°C.

[0098] After 5 months of storage, some fruits were severely wilted, and the seed viability was 20%.

[0099] Comparative Example 11

[0100] The difference between this comparative example and Example 1 is that it was stored at 4°C.

[0101] After 5 months of storage, the seed viability was 0.

[0102] Comparative Example 12

[0103] The difference between this comparative example and Example 1 is that styrene-butadiene latex was first applied to the surface of the fruit stalk and branches. After the latex dried, a mixture of methyl methacrylate, copper acetate, benzyl methacrylate, and pyraclostrobin was sprayed onto the surface of the fruit, fruit stalk, and branches. After drying, a mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil was sprayed.

[0104] After 5 months of storage, the seed viability was 63%. Because a protective layer was first applied to the fruit stalks and branches, the subsequent spraying of a mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil could not effectively kill the unhatched fruit fly eggs under the epidermis of the fruit stalks and branches. During the storage process, hatched fruit flies were observed, causing some fruits and seeds to rot, and the viability of the remaining seeds also decreased significantly.

[0105] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method of storing mango seeds, characterized by, The process includes the following steps: 77-84 days after mango fruit set, cut off the mango fruit with embryo along with the fruit stalk and branch. Spray with a mixture of pyraclostrobin + high-efficiency cyhalothrin + chlorothalonil. After drying, apply styrene-butadiene latex to the surface of the fruit stalk and branch. After the latex dries, spray the fruit, fruit stalk and branch surface again with a mixture of guanidine copper acetate + benzoyl pyraclostrobin. After drying, place the mango fruit with petioles and branches in a sealed box and refrigerate at 10-14℃. The mango in question is the Golden Phoenix mango; The main body of the sealed box is divided into an upper storage space and a lower water-proof space by a perforated partition. In the storage space, reduced iron powder is placed on the partition, then a gauze layer is laid on top, and then mango fruits with petioles and branches are placed on top. The fruits are covered with a gauze layer, and then coconut shell activated carbon is placed on the gauze layer. Place 10-15g of chlorine dioxide effervescent tablets in the water-proof space at the bottom of the sealed box; The sealed box is a foam sealed box, with foam blocks placed at the four corners of the water-proof space. The partition is a foam board placed on the foam blocks, and the foam board has 4-9 holes with a hole diameter of 1-1.5cm. The mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is prepared as follows: pyraclostrobin is a suspension with 30% active ingredient, diluted 300-400 times with water; lambda-cyhalothrin is a microcapsule suspension with 10% active ingredient, diluted 150-200 times with water; and chlorothalonil is a suspension with 40% active ingredient, diluted 100-150 times with water. The aforementioned guanidine·copper acetate + benzyl·pyrazole ester mixture is prepared as follows: guanidine·copper acetate is a wettable powder with 20% active ingredient, diluted 50-80 times with water; benzyl·pyrazole ester is a suspension with 32% active ingredient, diluted 200-300 times with water. The styrene-butadiene latex has a solid content of 50% and a viscosity of 1300-2000 mPa·s.

2. The mango seed storage method according to claim 1, characterized in that, Replace the reduced iron powder and coconut shell activated carbon in the sealed box once a month, and remove the moisture under the partition.

3. The mango seed storage method according to claim 1, characterized in that, When the Golden Phoenix mango is in bloom, cut off the top 2 / 3 of the inflorescence, leaving the small flowers at the base of the inflorescence that bloom at the same time. Hang the tags during the peak flowering period. After flowering and fruit setting, manage it as usual. Remove the non-embryo fruits 40-45 days after fruit setting.

4. The mango seed storage method according to claim 1, characterized in that, After the mango fruit is cut off along with the stem and branch, with the top of the fruit facing upwards, leave it for 1-1.5 hours until the secretion at the cut stop, then spray with a mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil.