A reagent and kit for quantitative detection of egg white allergen-specific IgE
By selecting specific biotin labeling methods and compounding ratios for different allergenic proteins and optimizing the composition of the preservation solution, the problem of inconsistency between egg white allergen detection results and clinical results has been solved, achieving higher detection accuracy and sensitivity.
CN116243004BActive Publication Date: 2026-06-16SUZHOU HAOOUBO BIOPHARML CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SUZHOU HAOOUBO BIOPHARML CO LTD
- Filing Date
- 2022-12-26
- Publication Date
- 2026-06-16
AI Technical Summary
⚠Technical Problem
The existing egg white allergen magnetic particle chemiluminescence reagent kits do not yield ideal results in terms of consistency with clinical findings, resulting in insufficient detection accuracy and sensitivity.
⚗Method used
By selecting different biotin labeling methods for different allergenic proteins and optimizing their compounding ratio, a mixed antigen reagent for multiple allergenic proteins is formed. Combined with a specific preservation solution, the consistency between test results and clinical symptoms is improved.
🎯Benefits of technology
It improves the accuracy and sensitivity of test results, reduces batch-to-batch variability, makes test results closer to clinical results, and enhances batch-to-batch consistency.
✦ Generated by Eureka AI based on patent content.
Abstract
The application provides a reagent and kit for quantitatively detecting chicken egg protein allergen specific IgE. The kit comprises biotin-labeled chicken egg protein allergen and a preservative solution, the biotin-labeled chicken egg protein allergen comprises Sulfo-NHS-biotin-labeled ovomucin, Sulfo-NHS-LC-biotin-labeled ovalbumin, Sulfo-NHS-biotin-labeled ovotransferrin and Sulfo-NHS-PEG4-biotin-labeled lysozyme with a mass ratio of (0.5-1):1:(0.5-1):0.5, and the preservative solution is a phosphate buffer solution containing 1-2 wt% BSA, 1-3 wt% glycerol and 2-5 wt% mannitol. The reagent has higher detection rate, detection sensitivity and reliability, the detection result has higher consistency with clinical symptoms, and the batch consistency of the finished product is better.
Need to check novelty before this filing date? Find Prior Art