A method for improving the survival rate of seedling grafting and promoting well-developed roots

Abstract

A method for improving the survival rate of seedling grafting and promoting well-developed roots, comprising the following steps: (1) selecting budstock seedlings; (2) pre-treating the budstock seedlings; (3) pre-treating the elite mother trees and selecting grafting buds; (4) grafting; (5) managing the grafted seedlings. Before grafting the budstock seedlings and before taking grafting buds from the elite mother trees, they are pre-treated with the budstock seedling pre-treatment solution and the elite mother tree pre-treatment solution respectively. After grafting, a grafting healing agent is coated around the grafting incision. After one month of grafting, a root-promoting fertilizer is applied to the roots. When raising seedlings by the method of the present invention, the seedling age is smaller, the survival rate is over 95%, the emergence time is short, and the macadamia grafted seedlings grow vigorously and have good growth potential during subsequent field planting.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural planting, and particularly relates to a method for improving the grafting survival rate of seedlings and promoting well-developed roots. Background Art

[0002] Macadamia nut alias: Queensland chestnut, Australian walnut, Hawaiian nut, Queensland nut, is a tree nut native to Australia. Macadamia nut belongs to evergreen arbor, dicotyledon. The tree crown is tall and large, with 3 - 4 leaves whorled, lanceolate, leathery, smooth, and with spiny serrations on the edge. The raceme is axillary, the flower is cream-colored, the fruit is spherical, the pericarp is leathery, the endocarp is hard, and the kernel is cream-colored to light brown. It is suitable to grow in areas with mild, humid climate and little wind. Macadamia nut has the characteristics of high oil content, rich nutrition, unique flavor, etc. The pulp is crispy, smooth and delicious, with a sweet and greasy milk fragrance, and enjoys the reputation of "the king of nuts in the world". It has preventive effects on human aging, diabetes, cancer, cardiovascular diseases, etc. Macadamia nut is the second largest agriculture and forestry industry after the walnut industry. In the process of seedling cultivation, two different methods of cutting seedling cultivation and grafting seedling cultivation can be used for macadamia nut.

[0003] Currently, for grafting seedling cultivation, the mainly selected seedling age is about 12 months, and the rootstock seedlings with a root diameter of 0.8 - 1.2 cm are used for grafting. Affected by various factors such as season, weather, thin subcutaneous cambium, and grafting techniques, the survival rate of grafting seedling cultivation is very low, only about 50%, and the seedling cultivation time is more than one and a half years. This has the problems of long seedling cultivation time and low seedling cultivation efficiency. Summary of the Invention

[0004] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a method for improving the grafting survival rate of seedlings and promoting well-developed roots, in which the stem diameter of the bud rootstock seedling reaches more than 0.3 cm, the seedling cultivation time is short, the survival rate is high, and the seedling cultivation efficiency is high.

[0005] In order to achieve the above purpose, the technical scheme adopted by the present invention is as follows: A method for improving the grafting survival rate of seedlings and promoting well-developed roots, comprising the following steps:

[0006] (1) Selecting bud rootstock seedlings: Selecting healthy macadamia nut seedlings with a stem diameter of 0.3 cm - 1.2 cm as bud rootstock seedlings;

[0007] (2) Pretreatment of bud rootstock seedlings: 5 - 7 days before grafting, applying 80 - 100 g / plant of the pretreatment solution for bud rootstock seedlings to the roots of the bud rootstock seedlings; The pretreatment solution for bud rootstock seedlings is calculated by weight parts and includes: 1000 parts of biogas slurry, 0.2 - 0.3 parts of 1.8 wt% sodium nitrophenolate aqueous solution, 3 - 5 parts of sugar alcohol boron, 35 - 38 parts of potassium dihydrogen phosphate, and 5 - 7 parts of ammonium molybdate;

[0008] (3)Pre-treatment of elite mother trees and selection of grafting buds: Select elite mother trees of Macadamia integrifolia without diseases and pests. Three to five days before grafting, spray the whole tree with the pre-treatment solution for elite mother trees, and then select non-lignified tender buds as grafting buds; the pre-treatment solution for elite mother trees is obtained by diluting the pre-treatment solution for budstock seedlings by 10 to 15 times;

[0009] (4)Grafting: Graft the grafting buds onto the budstock seedlings described in step (2), apply a grafting healing agent around the grafting incision, and then wrap and fix the grafting incision with a clean plastic film to obtain Macadamia integrifolia grafted seedlings; the grafting healing agent, by weight, includes: 1000 parts of erythromycin ointment, 80 to 90 parts of sulfur powder, 6 to 8 parts of amino acid trace element chelate, 0.1 to 0.2 parts of indoleacetic acid, and 0.2 to 0.3 parts of gibberellin;

[0010] The amino acid trace element chelate, by weight, includes: 45 to 50 parts of amino acid chelated iron, 35 to 40 parts of amino acid chelated copper, 25 to 30 parts of amino acid chelated magnesium, and 15 to 20 parts of amino acid chelated zinc;

[0011] (5)Management of grafted seedlings:

[0012] 5.1) Light management: Build a shading net with a shading rate of 60% to 65% above the seedbed of Macadamia integrifolia grafted seedlings;

[0013] 5.2) Temperature and humidity control: Cover a layer of sawdust powder with a thickness of 4 to 5 cm on the seedbed of Macadamia integrifolia grafted seedlings, control the temperature of the seedbed of Macadamia integrifolia grafted seedlings at 18 to 26 °C, and the humidity at 85% to 90%;

[0014] 5.3) Fertilization: Apply root-promoting fertilizer one month after grafting, and the fertilization depth is 10 to 15 cm; the root-promoting fertilizer, by weight, includes: 100 to 120 parts of decomposed poultry and livestock manure, 10 to 13 parts of agricultural amino acid raw powder, 8 to 10 parts of potassium dihydrogen phosphate, 3 to 5 parts of amino acid trace element chelate, and 0.1 to 0.2 parts of acephate.

[0015] Further, six to seven days before grafting in step (2), apply 90 to 100 g / plant of the pre-treatment solution for budstock seedlings to the roots of the budstock seedlings; the pre-treatment solution for budstock seedlings, by weight, includes: 1000 parts of biogas slurry, 0.25 to 0.3 parts of 1.8 wt% sodium nitrophenolate aqueous solution, 4 to 5 parts of mannitol boron, 36 to 38 parts of potassium dihydrogen phosphate, and 6 to 7 parts of ammonium molybdate.

[0016] Further, the pre-treatment solution for budstock seedlings also includes 4 to 6 parts by weight of manganese sulfate.

[0017] Further, in the step (3), select high-quality mother trees of Macadamia integrifolia without diseases and pests. Four to five days before grafting, spray the pretreatment liquid for high-quality mother trees on the whole tree to make the leaves and stems of the high-quality mother trees of Macadamia integrifolia fully moist, and then select non-lignified tender buds as grafting buds; the pretreatment liquid for high-quality mother trees is obtained by diluting the pretreatment liquid for budstock seedlings by 12 to 15 times.

[0018] Further, the grafting healing agent, by weight, comprises: 1000 parts of erythromycin ointment, 85 to 90 parts of sulfur powder, 7 to 8 parts of amino acid trace element chelate, 0.15 to 0.2 part of indoleacetic acid, and 0.25 to 0.3 part of gibberellin;

[0019] The amino acid trace element chelate, by weight, comprises: 47 to 50 parts of amino acid chelated iron, 36 to 40 parts of amino acid chelated copper, 28 to 30 parts of amino acid chelated magnesium, and 18 to 20 parts of amino acid chelated zinc.

[0020] Further, in the step 5.2), cover a layer of sawdust powder with a thickness of 4.5 to 5 cm on the seedbed of Macadamia integrifolia grafted seedlings, control the temperature of the seedbed of Macadamia integrifolia grafted seedlings at 20 to 26 °C, and control the humidity at 87 to 90%.

[0021] Further, in the step 5.3), apply 120 to 150 g / plant of root-promoting fertilizer.

[0022] Further, the root-promoting fertilizer in the step 5.3), by weight, comprises: 110 to 120 parts of decomposed livestock and poultry manure, 11 to 13 parts of agricultural amino acid raw powder, 9 to 10 parts of potassium dihydrogen phosphate, 4 to 5 parts of amino acid trace element chelate, and 0.15 to 0.2 part of acephate.

[0023] Further, 10 to 15 days after applying the root-promoting fertilizer in the step 5.3), apply a peat soil mixture by root application, and the root application depth is 5 to 8 cm; the peat soil mixture, by weight, comprises: 100 parts of peat soil, 25 to 30 parts of zeolite powder, and 30 to 35 parts of edible mushroom residue; the dosage of the peat soil mixture is 350 to 400 g / plant.

[0024] Further, after applying the peat soil mixture by root application in the step 5.3), apply orange peel water by root application.

[0025] A method for improving the grafting survival rate of seedlings and promoting the development of developed root systems. Before grafting the budstock seedlings and before taking grafting buds from the elite mother trees, they are pretreated with a budstock seedling pretreatment solution and an elite mother tree pretreatment solution respectively. Compound sodium nitrophenolate is added to the pretreatment solution as a powerful cell activator. After contacting with the budstock seedlings and the elite mother trees, it can quickly penetrate into the plant body, promote the protoplasm flow of cells, improve cell vitality, help absorb potassium, molybdenum in the pretreatment solution and major and trace elements in the soil, and effectively improve the grafting survival rate of Macadamia integrifolia seedlings; apply a grafting healing agent around the grafting incision. Erythromycin ointment and sulfur powder in the grafting healing agent are used to prevent bacterial infection in the air. Added indoleacetic acid and gibberellin are used as exogenous hormones to stimulate the cells near the grafting incision to accelerate division, accelerate the incision healing, and the amino acid trace element chelate effectively supplements elements such as iron, iron, magnesium, and zinc, promotes the growth of callus, and effectively improves the grafting survival rate of Macadamia integrifolia seedlings; apply a root-promoting fertilizer to the roots after a full month of grafting, continuously supplement organic matter, amino acids, potassium, iron, iron, magnesium, zinc and other elements, effectively promote the root growth of the budstock seedlings, greatly increase the content of flavonoids and polyphenols in the roots of the budstock seedlings, effectively improve the grafting survival rate of Macadamia integrifolia seedlings, and at the same time effectively promote the development of the root systems of Macadamia integrifolia seedlings, thereby effectively driving the vigorous growth of Macadamia integrifolia grafted seedlings; using the method of the present invention for seedling cultivation, the seedling age is smaller, the survival rate is over 95%, the emergence time is short, and the seedlings can be outplanted half a year after grafting. The outplanted seedlings have developed root systems, and the content of various flavonoid components and polyphenol components in the roots has increased significantly. When the Macadamia integrifolia grafted seedlings are planted in the field later, they grow stronger and have better growth vigor.

[0026] A method for improving the grafting survival rate of seedlings and promoting the development of developed root systems. The budstock seedling pretreatment solution further includes manganese sulfate to supplement manganese elements, which can regulate the oxidation-reduction reaction in the budstock seedlings, is beneficial to the synthesis of amino acids and proteins, promotes root growth, thickens the root and stem of the budstock seedlings, promotes the development of the root systems of Macadamia integrifolia seedlings, and at the same time improves the grafting survival rate of Macadamia integrifolia seedlings; apply a peat soil mixture to the roots. The cooperation of peat soil, zeolite powder and edible mushroom residues can loosen the soil texture and increase the soil organic matter content at the same time, which can effectively promote the root growth of the budstock seedlings; peat soil is slightly acidic, and applying orange peel water with weak acidity can not only supplement the nutrient elements required for the growth of the budstock seedlings, but also effectively control pests, and at the same time can increase the acid-aluminum tolerance of the budstock seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is the upper part of the detection result diagram of the root component content before grafting the budstock seedlings in Example 1;

[0028] Figure 2 It is the lower part of the detection result diagram of the root component content before grafting the budstock seedlings in Example 1;

[0029] Figure 3For the detection result diagram of the root system component content when the budstock seedlings are outplanted in Example 1 above;

[0030] Figure 4 For the detection result diagram of the root system component content when the budstock seedlings are outplanted in Example 1 below. Detailed implementation method

[0031] The following examples can help those skilled in the art to understand the present invention more comprehensively, but the present invention cannot be limited in any way.

[0032] A method for improving the grafting survival rate of seedlings and promoting the development of the root system according to the present invention. The seeds of the budstock seedlings are selected from healthy Macadamia integrifolia plants that are strong in growth, healthy and disease-free, and have high and stable yields. The variety of the budstock seedlings and the variety of the elite mother trees can be selected according to actual needs. The following examples give the selection and cultivation of several common varieties, and the variety ranges of the budstock seedlings and the elite mother trees should not be limited thereby.

[0033] A method for improving the grafting survival rate of seedlings and promoting the development of the root system according to the present invention. The preparation method of the 1.8wt% sodium nitrophenolate aqueous solution is as follows: Weigh 1.8 g of sodium nitrophenolate technical powder, and then add 98.2 g of water and dilute evenly.

[0034] A method for improving the grafting survival rate of seedlings and promoting the development of the root system according to the present invention. The preparation method of the orange peel water is as follows: Take fresh orange peels and cut them into pieces. Add 1000 g of clear water to every 50 g of fresh orange peels and soak for 24 hours.

[0035] A method for improving the grafting survival rate of seedlings and promoting the development of the root system according to the present invention. The composition ratio of the amino acid trace element chelate in the root-promoting fertilizer is the same as that of the amino acid trace element chelate in the grafting healing agent. The amino acid chelated iron, amino acid chelated copper, amino acid chelated magnesium, and amino acid chelated zinc in the amino acid trace element chelate can all be purchased on the market.

[0036] Example 1

[0037] A method for improving the grafting survival rate of seedlings and promoting the development of the root system, comprising the following steps:

[0038] (1) Selecting budstock seedlings: Select healthy Macadamia integrifolia seedlings with a stem diameter of 0.3 cm as budstock seedlings; the variety of the budstock seedlings is No. 695;

[0039] (2) Pretreatment of budstock seedlings: 5 days before grafting, apply 80 g / plant of the budstock seedling pretreatment solution to the roots of the budstock seedlings; the budstock seedling pretreatment solution is calculated by weight and includes: 1000 parts of biogas slurry, 0.2 part of 1.8wt% sodium nitrophenolate aqueous solution, 3 parts of sorbitol boron, 35 parts of potassium dihydrogen phosphate, 5 parts of ammonium molybdate, and 4 parts of manganese sulfate;

[0040] (3)Pre-treatment of elite mother trees and selection of grafting buds: Select elite mother trees of Macadamia integrifolia without diseases and pests. The variety of the elite mother trees is H2. Three days before grafting, spray the pre-treatment solution for elite mother trees on the whole tree to make the leaves and stems of the elite mother trees of Macadamia integrifolia fully moist. Then select non-lignified tender buds as grafting buds. After the grafting buds are intercepted, they should be sprayed with cold water to keep them moist, and at the same time, shade treatment should be carried out to avoid wilting. The pre-treatment solution for elite mother trees is obtained by diluting the pre-treatment solution for rootstock seedlings by 10 times.

[0041] (4)Grafting: Within one month before grafting, sufficient fertilizers should be applied to the rootstock seedlings, and weeding and pest control should be actively carried out. Water should be thoroughly watered three days before grafting. Graft the grafting buds onto the rootstock seedlings described in step (2), apply grafting healing agent around the grafting incision, and then wrap and fix the grafting incision with clean plastic film to obtain Macadamia integrifolia grafted seedlings. The grafting healing agent, by weight, includes: 1000 parts of erythromycin ointment, 80 parts of sulfur powder, 6 parts of amino acid trace element chelate, 0.1 part of indoleacetic acid, and 0.2 part of gibberellin.

[0042] The amino acid trace element chelate, by weight, includes: 45 parts of amino acid chelated iron, 35 parts of amino acid chelated copper, 25 parts of amino acid chelated magnesium, and 15 parts of amino acid chelated zinc.

[0043] (5)Management of grafted seedlings:

[0044] 5.1)Light management: Build a shading net with a shading rate of 60% above the seedbed of Macadamia integrifolia grafted seedlings.

[0045] 5.2)Temperature and humidity control: Cover a layer of sawdust powder with a thickness of 4 cm on the seedbed of Macadamia integrifolia grafted seedlings, control the temperature of the seedbed of Macadamia integrifolia grafted seedlings at 18 °C, and control the humidity at 85%.

[0046] 5.3)Fertilization: Apply 120 g / plant of root-promoting fertilizer to the roots one month after grafting, and the fertilization depth is 10 cm. Ten days after applying the root-promoting fertilizer, apply a mixture of peat soil, and the root application depth is 5 cm. After applying the mixture of peat soil, apply orange peel water. The mixture of peat soil, by weight, includes: 100 parts of peat soil, 25 parts of zeolite powder, and 30 parts of edible mushroom residue. The dosage of the mixture of peat soil is 350 g / plant. The root-promoting fertilizer, by weight, includes: 100 parts of decomposed livestock manure, 10 parts of agricultural amino acid raw powder, 8 parts of potassium dihydrogen phosphate, 3 parts of amino acid trace element chelate, and 0.1 part of acephate.

[0047] Example 2

[0048] A method for improving the grafting survival rate of seedlings and promoting the development of root systems, including the following steps:

[0049] (1)Selecting the budstock seedlings: Select healthy Macadamia integrifolia seedlings with a stem diameter of 1.2 cm as the budstock seedlings; the variety of the budstock seedlings is Guire No. 1;

[0050] (2)Pre-treatment of the budstock seedlings: Seven days before grafting, apply 100 g / plant of the pre-treatment solution for budstock seedlings to the roots of the budstock seedlings; the pre-treatment solution for budstock seedlings, by weight, includes: 1000 parts of biogas slurry, 0.3 part of 1.8 wt% sodium nitrophenolate aqueous solution, 5 parts of mannitol boron, 38 parts of potassium dihydrogen phosphate, 7 parts of ammonium molybdate, and 6 parts of manganese sulfate;

[0051] (3)Pre-treatment of the elite mother trees and selection of grafting buds: Select elite Macadamia integrifolia mother trees without diseases and pests. The variety of the elite mother trees is OC. Five days before grafting, spray the whole tree with the pre-treatment solution for elite mother trees to make the leaves and stems of the elite Macadamia integrifolia mother trees fully moist. Then select non-lignified tender buds as grafting buds. After the grafting buds are intercepted, spray them with cold water to keep them moist, and at the same time, carry out shading treatment to avoid wilting; the pre-treatment solution for elite mother trees is obtained by diluting the pre-treatment solution for budstock seedlings by 15 times;

[0052] (4)Grafting: One month before grafting, sufficient fertilizers need to be applied to the budstock seedlings, and weeding and pest control work should be actively carried out. Water should be fully irrigated three days before grafting; Graft the grafting buds onto the budstock seedlings described in step (2), apply the grafting healing agent around the grafting incision, and then wrap and fix the grafting incision with a clean plastic film to obtain Macadamia integrifolia grafted seedlings; the grafting healing agent, by weight, includes: 1000 parts of erythromycin ointment, 90 parts of sulfur powder, 8 parts of amino acid trace element chelate, 0.2 part of indoleacetic acid, and 0.3 part of gibberellin;

[0053] The amino acid trace element chelate, by weight, includes: 50 parts of amino acid chelated iron, 40 parts of amino acid chelated copper, 30 parts of amino acid chelated magnesium, and 20 parts of amino acid chelated zinc;

[0054] (5)Management of grafted seedlings:

[0055] 5.1)Light management: Build a shading net with a shading degree of 65% above the seedbed of Macadamia integrifolia grafted seedlings;

[0056] 5.2)Temperature and humidity control: Cover a layer of sawdust powder with a thickness of 5 cm on the seedbed of Macadamia integrifolia grafted seedlings, control the temperature of the seedbed of Macadamia integrifolia grafted seedlings at 26 °C, and control the humidity at 90%;

[0057] 5.3) Fertilization: After one full month of grafting, apply 150 g of root-promoting fertilizer per plant to the roots, with the fertilization depth at 15 cm; 15 days after applying the root-promoting fertilizer, apply a peat soil mixture to the roots, with the fertilization depth at 8 cm; after applying the peat soil mixture, apply orange peel water to the roots; the peat soil mixture, by weight, includes: 100 parts of peat soil, 30 parts of zeolite powder, and 35 parts of edible mushroom residue; the dosage of the peat soil mixture is 400 g per plant; the root-promoting fertilizer, by weight, includes: 120 parts of decomposed livestock manure, 13 parts of agricultural amino acid raw powder, 10 parts of potassium dihydrogen phosphate, 5 parts of amino acid trace element chelate, and 0.2 part of acephate.

[0058] Example 3

[0059] A method for improving the grafting survival rate of seedlings and promoting root development, comprising the following steps:

[0060] (1) Selecting budstock seedlings: Select healthy macadamia nut seedlings with a stem diameter of 0.5 cm as budstock seedlings; the variety of the budstock seedlings is No. A16;

[0061] (2) Pretreatment of budstock seedlings: 6 days before grafting, apply 90 g of pretreatment liquid for budstock seedlings per plant to the roots of the budstock seedlings; the pretreatment liquid for budstock seedlings, by weight, includes: 1000 parts of biogas slurry, 0.25 part of 1.8 wt% sodium nitrophenolate aqueous solution, 4 parts of sorbitol boron, 36 parts of potassium dihydrogen phosphate, 6 parts of ammonium molybdate, and 5 parts of manganese sulfate;

[0062] (3) Pretreatment of elite mother trees and selection of grafting buds: Select healthy macadamia nut elite mother trees without diseases and pests. The variety of the elite mother trees is No. OC. 4 days before grafting, spray the pretreatment liquid for elite mother trees all over the tree to make the leaves and stems of the macadamia nut elite mother trees fully wet. Then select non-lignified tender buds as grafting buds. After the grafting buds are intercepted, spray them with cold water to keep them wet, and at the same time, perform shading treatment to avoid wilting; the pretreatment liquid for elite mother trees is obtained by diluting the pretreatment liquid for budstock seedlings by 12 times;

[0063] (4) Grafting: Within 1 month before grafting, sufficient fertilizer needs to be applied to the budstock seedlings, and weeding and pest control should be actively carried out. Water should be fully irrigated three days before grafting; graft the grafting buds onto the budstock seedlings described in step (2), apply grafting healing agent around the grafting incision, and then wrap and fix the grafting incision with clean plastic film to obtain macadamia nut grafted seedlings; the grafting healing agent, by weight, includes: 1000 parts of erythromycin ointment, 85 parts of sulfur powder, 7 parts of amino acid trace element chelate, 0.15 part of indoleacetic acid, and 0.25 part of gibberellin;

[0064] The amino acid trace element chelate, by weight, includes: 47 parts of amino acid chelated iron, 36 parts of amino acid chelated copper, 28 parts of amino acid chelated magnesium, and 18 parts of amino acid chelated zinc;

[0065] (5)Graft seedling management:

[0066] 5.1) Light management: Build a shading net with a shading degree of 62% above the seedbed of Macadamia integrifolia grafted seedlings.

[0067] 5.2) Temperature and humidity control: Cover a layer of sawdust powder with a thickness of 4.5 cm on the seedbed of Macadamia integrifolia grafted seedlings, control the temperature of the seedbed of Macadamia integrifolia grafted seedlings at 20 °C, and control the humidity at 87%.

[0068] 5.3) Fertilization: Apply 130 g / plant of root-promoting fertilizer to the roots 1 month after grafting, and the fertilization depth is 12 cm; 13 days after applying the root-promoting fertilizer to the roots, apply a mixture of peat soil to the roots, and the root application depth is 6 cm; after applying the mixture of peat soil to the roots, apply orange peel water; the mixture of peat soil includes, by weight: 100 parts of peat soil, 27 parts of zeolite powder, and 32 parts of edible mushroom residue; the dosage of the mixture of peat soil is 370 g / plant; the root-promoting fertilizer includes, by weight: 110 parts of decomposed livestock manure, 11 parts of agricultural amino acid raw powder, 9 parts of potassium dihydrogen phosphate, 4 parts of amino acid trace element chelate, and 0.15 part of acephate.

[0069] Example 4

[0070] A method for improving the grafting survival rate of seedlings and promoting the development of developed roots, comprising the following steps:

[0071] (1)Selecting budstock seedlings: Select healthy Macadamia integrifolia seedlings with a stem diameter of 0.5 cm as budstock seedlings; the variety of the budstock seedlings is No. A16.

[0072] (2)Pre-treatment of budstock seedlings: 6 days before grafting, apply 90 g / plant of pre-treatment liquid for budstock seedlings to the roots of the budstock seedlings; the pre-treatment liquid for budstock seedlings includes, by weight: 1000 parts of biogas slurry, 0.25 part of 1.8 wt% sodium nitrophenolate aqueous solution, 4 parts of mannitol boron, 36 parts of potassium dihydrogen phosphate, and 6 parts of ammonium molybdate.

[0073] (3)Pre-treatment of elite mother trees and selection of grafting buds: Select elite mother trees of Macadamia integrifolia without diseases and pests. The variety of the elite mother trees is No. OC. 4 days before grafting, spray the whole tree with the pre-treatment liquid for elite mother trees to make the leaves and stem surfaces of the elite mother trees of Macadamia integrifolia fully wet. Then select non-lignified tender buds as grafting buds. After the grafting buds are intercepted, they should be sprayed with cold water to keep them moist, and at the same time, they should be shaded to avoid wilting; the pre-treatment liquid for elite mother trees is obtained by diluting the pre-treatment liquid for budstock seedlings by 12 times.

[0074] (4) Grafting: Within 1 month before grafting, sufficient fertilizers should be applied to the budstock seedlings, and weeding and pest control should be actively carried out. Water should be thoroughly irrigated three days before grafting. Graft the grafting bud onto the budstock seedlings described in step (2), apply a grafting healing agent around the grafting incision, and then wrap and fix the grafting incision with a clean plastic film to obtain Macadamia integrifolia grafted seedlings. The grafting healing agent, by weight, includes: 1000 parts of erythromycin ointment, 85 parts of sulfur powder, 7 parts of amino acid trace element chelate, 0.15 parts of indoleacetic acid, and 0.25 parts of gibberellin;

[0075] The amino acid trace element chelate, by weight, includes: 47 parts of amino acid chelated iron, 36 parts of amino acid chelated copper, 28 parts of amino acid chelated magnesium, and 18 parts of amino acid chelated zinc;

[0076] (5) Management of grafted seedlings:

[0077] 5.1) Light management: Build a shading net with a shading rate of 62% above the seedbed of Macadamia integrifolia grafted seedlings;

[0078] 5.2) Temperature and humidity control: Cover a layer of sawdust powder with a thickness of 4.5 cm on the seedbed of Macadamia integrifolia grafted seedlings, control the temperature of the seedbed of Macadamia integrifolia grafted seedlings at 20 °C, and the humidity at 87%;

[0079] 5.3) Fertilization: Apply 130 g / plant of root-promoting fertilizer to the roots 1 month after grafting, and the fertilization depth is 12 cm; 13 days after applying the root-promoting fertilizer, apply a peat soil mixture to the roots, and the root application depth is 6 cm; after applying the peat soil mixture, apply orange peel water to the roots. The peat soil mixture, by weight, includes: 100 parts of peat soil, 27 parts of zeolite powder, and 32 parts of edible mushroom residue; the dosage of the peat soil mixture is 370 g / plant. The root-promoting fertilizer, by weight, includes: 110 parts of decomposed livestock manure, 11 parts of agricultural amino acid raw powder, 9 parts of potassium dihydrogen phosphate, 4 parts of amino acid trace element chelate, and 0.15 parts of acephate.

[0080] Example 5

[0081] A method for improving the grafting survival rate of seedlings and promoting well-developed roots, comprising the following steps:

[0082] (1) Selecting budstock seedlings: Select healthy Macadamia integrifolia seedlings with a stem diameter of 0.5 cm as budstock seedlings; the variety of the budstock seedlings is No. A16;

[0083] (2) Pretreatment of budstock seedlings: 6 days before grafting, apply 90 g / plant of budstock seedling pretreatment liquid to the roots of the budstock seedlings. The budstock seedling pretreatment liquid, by weight, includes: 1000 parts of biogas slurry, 0.25 parts of 1.8 wt% sodium nitrophenolate aqueous solution, 4 parts of sugar alcohol boron, 36 parts of potassium dihydrogen phosphate, 6 parts of ammonium molybdate, and 5 parts of manganese sulfate;

[0084] (3)Pre-treatment of elite mother trees and selection of grafting buds: Select elite mother trees of Macadamia integrifolia without diseases and pests. The variety of the elite mother trees is the OC variety. Four days before grafting, spray the pre-treatment solution for elite mother trees on the whole tree to make the leaves and stems of the elite mother trees of Macadamia integrifolia fully moist. Then select non-lignified tender buds as grafting buds. After the grafting buds are intercepted, spray them with cold water to keep them moist, and at the same time, carry out shading treatment to avoid wilting; the pre-treatment solution for elite mother trees is obtained by diluting the pre-treatment solution for rootstock seedlings by 12 times;

[0085] (4)Grafting: One month before grafting, sufficient fertilizers need to be applied to the rootstock seedlings, and weeding and pest control work should be actively carried out. Water should be fully irrigated three days before grafting; Graft the grafting buds onto the rootstock seedlings described in step (2), apply grafting healing agent around the grafting incision, and then wrap and fix the grafting incision with clean plastic film to obtain Macadamia integrifolia grafted seedlings; The grafting healing agent, by weight, includes: 1000 parts of erythromycin ointment, 85 parts of sulfur powder, 7 parts of amino acid trace element chelate, 0.15 parts of indoleacetic acid, and 0.25 parts of gibberellin;

[0086] The amino acid trace element chelate, by weight, includes: 47 parts of amino acid chelated iron, 36 parts of amino acid chelated copper, 28 parts of amino acid chelated magnesium, and 18 parts of amino acid chelated zinc;

[0087] (5)Management of grafted seedlings:

[0088] 5.1) Light management: Build a shading net with a shading degree of 62% above the seedbed of Macadamia integrifolia grafted seedlings;

[0089] 5.2) Temperature and humidity control: Cover a layer of sawdust powder with a thickness of 4.5 cm on the seedbed of Macadamia integrifolia grafted seedlings, control the temperature of the seedbed of Macadamia integrifolia grafted seedlings at 20 °C, and control the humidity at 87%;

[0090] 5.3) Fertilization: Apply 130 g / plant of root-promoting fertilizer to the roots one month after grafting, and the fertilization depth is 12 cm; 13 days after applying the root-promoting fertilizer to the roots, apply the peat soil mixture to the roots, and the fertilization depth is 6 cm; The peat soil mixture, by weight, includes: 100 parts of peat soil, 27 parts of zeolite powder, and 32 parts of edible mushroom residue; The dosage of the peat soil mixture is 370 g / plant; The root-promoting fertilizer, by weight, includes: 110 parts of decomposed livestock manure, 11 parts of agricultural amino acid raw powder, 9 parts of potassium dihydrogen phosphate, 4 parts of amino acid trace element chelate, and 0.15 parts of acephate.

[0091] Example 6

[0092] A method for improving the grafting survival rate of seedlings and promoting the development of developed roots, including the following steps:

[0093] (1)Select the budstock seedlings: Select healthy Macadamia integrifolia seedlings with a stem diameter of 0.5 cm as the budstock seedlings; the variety of the budstock seedlings is No. A16;

[0094] (2)Pre-treatment of the budstock seedlings: Six days before grafting, apply 90 g / plant of the pre-treatment solution for budstock seedlings to the roots of the budstock seedlings; the pre-treatment solution for budstock seedlings, by weight, includes: 1000 parts of biogas slurry, 0.25 part of 1.8 wt% sodium nitrophenolate aqueous solution, 4 parts of sorbitol boron, 36 parts of potassium dihydrogen phosphate, 6 parts of ammonium molybdate, and 5 parts of manganese sulfate;

[0095] (3)Pre-treatment of the elite mother tree and selection of the grafting buds: Select healthy Macadamia integrifolia elite mother trees without pests and diseases. The variety of the elite mother trees is No. OC. Four days before grafting, spray the whole tree with the pre-treatment solution for elite mother trees to make the leaves and stem surfaces of the Macadamia integrifolia elite mother trees fully moist. Then select non-lignified tender buds as the grafting buds. After the grafting buds are intercepted, they should be sprayed with cold water to keep them moist, and at the same time, they should be shaded to avoid wilting; the pre-treatment solution for elite mother trees is obtained by diluting the pre-treatment solution for budstock seedlings 12 times;

[0096] (4)Grafting: One month before grafting, sufficient fertilizers should be applied to the budstock seedlings, and weeding and pest control should be actively carried out. Water should be fully irrigated three days before grafting; graft the grafting buds onto the budstock seedlings described in step (2), apply the grafting healing agent around the grafting incision, and then wrap and fix the grafting incision with a clean plastic film to obtain Macadamia integrifolia grafted seedlings; the grafting healing agent, by weight, includes: 1000 parts of erythromycin ointment, 85 parts of sulfur powder, 7 parts of amino acid trace element chelate, 0.15 part of indoleacetic acid, and 0.25 part of gibberellin;

[0097] The amino acid trace element chelate, by weight, includes: 47 parts of amino acid chelated iron, 36 parts of amino acid chelated copper, 28 parts of amino acid chelated magnesium, and 18 parts of amino acid chelated zinc;

[0098] (5)Management of grafted seedlings:

[0099] 5.1)Light management: Build a shading net with a shading degree of 62% above the seedbed of Macadamia integrifolia grafted seedlings;

[0100] 5.2)Temperature and humidity control: Cover a layer of sawdust powder with a thickness of 4.5 cm on the seedbed of Macadamia integrifolia grafted seedlings, control the temperature of the seedbed of Macadamia integrifolia grafted seedlings at 20 °C, and control the humidity at 87%;

[0101] 5.3)Fertilization: Apply 130 g / plant of root-promoting fertilizer to the roots one month after grafting, and the fertilization depth is 12 cm; the root-promoting fertilizer, by weight, includes: 110 parts of decomposed livestock manure, 11 parts of agricultural amino acid raw powder, 9 parts of potassium dihydrogen phosphate, 4 parts of amino acid trace element chelate, and 0.15 part of acephate.

[0102] Comparative Example 1

[0103] A method for improving the grafting survival rate of seedlings and promoting well-developed roots, comprising the following steps:

[0104] (1) Selecting budstock seedlings: Selecting healthy macadamia nut seedlings with a stem diameter of 0.5 cm as budstock seedlings; the variety of the budstock seedlings is No. A16;

[0105] (2) Pretreatment of elite mother trees and selection of grafting buds: Selecting disease- and pest-free elite macadamia nut mother trees, the variety of the elite mother trees is No. OC, selecting non-lignified tender buds as grafting buds, and after the grafting buds are intercepted, they need to be sprayed with cold water to keep them moist, and at the same time, shading treatment should be carried out to avoid wilting;

[0106] (3) Grafting: Within 1 month before grafting, it is necessary to apply sufficient fertilizer to the budstock seedlings, and actively do a good job in weeding and pest control. Three days before grafting, water should be thoroughly watered; graft the grafting buds onto the budstock seedlings described in step (1), apply a grafting healing agent around the grafting incision, and then wrap and fix the grafting incision with a clean plastic film to obtain macadamia nut grafted seedlings; the grafting healing agent is calculated by weight and includes: 1000 parts of erythromycin ointment, 85 parts of sulfur powder, 7 parts of amino acid trace element chelate, 0.15 part of indoleacetic acid, and 0.25 part of gibberellin;

[0107] The amino acid trace element chelate is calculated by weight and includes: 47 parts of amino acid chelated iron, 36 parts of amino acid chelated copper, 28 parts of amino acid chelated magnesium, and 18 parts of amino acid chelated zinc;

[0108] (4) Management of grafted seedlings:

[0109] 4.1) Light management: Build a shading net with a shading degree of 62% above the nursery bed of macadamia nut grafted seedlings;

[0110] 4.2) Temperature and humidity control: Cover a layer of sawdust powder with a thickness of 4.5 cm on the nursery bed of macadamia nut grafted seedlings, control the temperature of the nursery bed of macadamia nut grafted seedlings at 20°C, and control the humidity at 87%;

[0111] 4.3) Fertilization: Apply 130 g / plant of root-promoting fertilizer to the roots 1 month after grafting, and the fertilization depth is 12 cm; 13 days after applying the root-promoting fertilizer to the roots, apply a peat soil mixture to the roots, and the root application depth is 6 cm; after applying the peat soil mixture to the roots, apply orange peel water to the roots; the peat soil mixture is calculated by weight and includes: 100 parts of peat soil, 27 parts of zeolite powder, and 32 parts of edible mushroom residue; the dosage of the peat soil mixture is 370 g / plant; the root-promoting fertilizer is calculated by weight and includes: 110 parts of decomposed livestock manure, 11 parts of agricultural amino acid raw powder, 9 parts of potassium dihydrogen phosphate, 4 parts of amino acid trace element chelate, and 0.15 part of acephate.

[0112] Comparative Example 2

[0113] A method for improving the grafting survival rate of seedlings and promoting well-developed roots, comprising the following steps:

[0114] (1) Selecting budstock seedlings: Selecting healthy macadamia nut seedlings with a stem diameter of 0.5 cm as budstock seedlings; the variety of the budstock seedlings is No. A16;

[0115] (2) Pretreatment of budstock seedlings: 6 days before grafting, applying 90 g / plant of the budstock seedling pretreatment solution to the roots of the budstock seedlings; the budstock seedling pretreatment solution is composed of, by weight parts: 1000 parts of biogas slurry, 0.25 part of 1.8 wt% sodium nitrophenolate aqueous solution, 4 parts of sugar alcohol boron, 36 parts of potassium dihydrogen phosphate, 6 parts of ammonium molybdate, and 5 parts of manganese sulfate;

[0116] (3) Pretreatment of elite mother trees and selection of grafting buds: Selecting disease- and pest-free elite macadamia nut mother trees, the variety of the elite mother trees is No. OC. 4 days before grafting, spraying the elite mother tree pretreatment solution all over the tree to make the leaves and stem surfaces of the elite macadamia nut mother trees fully wet. Then selecting non-lignified tender buds as grafting buds. After the grafting buds are intercepted, they should be sprayed with cold water to keep them wet, and at the same time, shading treatment should be carried out to avoid wilting; the elite mother tree pretreatment solution is obtained by diluting the budstock seedling pretreatment solution 12 times;

[0117] (4) Grafting: Within 1 month before grafting, sufficient fertilizers need to be applied to the budstock seedlings, and weeding and pest control should be actively carried out. Water should be fully irrigated three days before grafting; grafting the grafting buds onto the budstock seedlings described in step (2), and then wrapping and fixing the grafting incision with clean plastic film to obtain macadamia nut grafted seedlings;

[0118] (5) Management of grafted seedlings:

[0119] 5.1) Light management: Building a shading net with a shading degree of 62% above the macadamia nut grafted seedling nursery bed;

[0120] 5.2) Temperature and humidity control: Covering a layer of sawdust powder with a thickness of 4.5 cm on the macadamia nut grafted seedling nursery bed, controlling the temperature of the macadamia nut grafted seedling nursery bed at 20 °C, and the humidity at 87%;

[0121] 5.3) Fertilization: After one month of grafting, applying 130 g / plant of root-promoting fertilizer by root application, and the fertilization depth is 12 cm; 13 days after applying the root-promoting fertilizer by root application, applying a peat soil mixture by root application, and the root application depth is 6 cm; after applying the peat soil mixture by root application, applying orange peel water by root application; the peat soil mixture is composed of, by weight parts: 100 parts of peat soil, 27 parts of zeolite powder, and 32 parts of edible mushroom residue; the dosage of the peat soil mixture is 370 g / plant; the root-promoting fertilizer is composed of, by weight parts: 110 parts of decomposed livestock manure, 11 parts of agricultural amino acid raw powder, 9 parts of potassium dihydrogen phosphate, 4 parts of amino acid trace element chelate, and 0.15 part of acephate.

[0122] Comparative Example 3

[0123] A method for improving the grafting survival rate of seedlings and promoting well-developed roots, comprising the following steps:

[0124] (1) Selecting rootstock seedlings: Select healthy Macadamia integrifolia seedlings with a stem diameter of 0.5 cm as rootstock seedlings; the variety of the rootstock seedlings is No. A16;

[0125] (2) Pretreatment of rootstock seedlings: Six days before grafting, apply 90 g / plant of the pretreatment solution for rootstock seedlings to the roots of the rootstock seedlings; the pretreatment solution for rootstock seedlings is composed of, by weight: 1000 parts of biogas slurry, 0.25 part of 1.8 wt% sodium nitrophenolate aqueous solution, 4 parts of borax in sugar alcohol, 36 parts of potassium dihydrogen phosphate, 6 parts of ammonium molybdate, and 5 parts of manganese sulfate;

[0126] (3) Pretreatment of elite mother trees and selection of grafting buds: Select elite Macadamia integrifolia mother trees without diseases and pests, the variety of the elite mother trees is No. OC. Four days before grafting, spray the pretreatment solution for elite mother trees on the whole tree to make the leaves and stem surfaces of the elite Macadamia integrifolia mother trees fully moist. Then select non-lignified tender buds as grafting buds. After the grafting buds are cut, they should be sprayed with cold water to keep them moist, and at the same time, shading treatment should be carried out to avoid wilting; the pretreatment solution for elite mother trees is obtained by diluting the pretreatment solution for rootstock seedlings by 12 times;

[0127] (4) Grafting: Within one month before grafting, sufficient fertilizers should be applied to the rootstock seedlings, and weeding and pest control should be actively carried out. Water should be fully irrigated three days before grafting; graft the grafting buds onto the rootstock seedlings described in step (2), apply a grafting healing agent around the grafting incision, and then wrap and fix the grafting incision with a clean plastic film to obtain Macadamia integrifolia grafted seedlings; the grafting healing agent is composed of, by weight: 1000 parts of erythromycin ointment, 85 parts of sulfur powder, 7 parts of amino acid trace element chelate, 0.15 part of indoleacetic acid, and 0.25 part of gibberellin;

[0128] The amino acid trace element chelate is composed of, by weight: 47 parts of amino acid chelated iron, 36 parts of amino acid chelated copper, 28 parts of amino acid chelated magnesium, and 18 parts of amino acid chelated zinc;

[0129] (5) Management of grafted seedlings:

[0130] 5.1) Light management: Build a shading net with a shading degree of 62% above the seedbed of Macadamia integrifolia grafted seedlings;

[0131] 5.2) Temperature and humidity control: Cover a layer of sawdust powder with a thickness of 4.5 cm on the seedbed of Macadamia integrifolia grafted seedlings, control the temperature of the seedbed of Macadamia integrifolia grafted seedlings at 20 °C, and control the humidity at 87%;

[0132] 5.3) Fertilization: After one month of grafting, apply 500 g / plant of decomposed pig manure to the roots, and the fertilization depth is 12 cm.

[0133] Table 1 below gives the statistical results of the grafting status of Macadamia integrifolia seedlings in Examples 1-6 and Comparative Examples 1-3.

[0134] Table 1

[0135] Serial number Survival rate of grafted seedlings / % Whether the flavonoid content in the roots increased significantly after grafting Whether the polyphenol content in the roots increased significantly after grafting Root depth of nursery stock / cm Shoot thickness / cm Example 1 96.8 Yes Yes ≥16.5 ≥1.35 Example 2 97.5 Yes Yes ≥16.3 ≥1.36 Example 3 98.4 Yes Yes ≥16.8 ≥1.38 Example 4 95.4 Yes Yes ≥15.5 ≥1.33 Example 5 95.8 Yes Yes ≥15.7 ≥1.30 Example 6 95.2 Yes Yes ≥15.0 ≥1.27 Control Example 1 73.6 No No ≥13.6 ≥1.03 Control Example 2 62.4 No No ≥14.5 ≥1.14 Control Example 3 78.9 No No ≥14.2 ≥1.18

[0136] Furthermore, comparing the changes in the contents of flavonoids and polyphenols in the roots before and after grafting in Examples 1-6 and Comparative Examples 1-3, it can be seen from the comparison that in Examples 1-6, the contents of eriodictyol glycoside and dihydroquercetin in the roots of the budstock seedlings before grafting were about 0.4 mg / kg, and the contents of components such as hesperetin, naringin, neohesperidin, rhoifolin, naringenin, luteolin, kaempferol, diosmetin, sinensetin, and nobiletin were about 0.2 mg / kg; after grafting, the contents of eriodictyol glycoside and dihydroquercetin in the roots of the budstock seedlings were 0.5 - 0.6 mg / kg, and the contents of components such as hesperetin, naringin, neohesperidin, rhoifolin, naringenin, luteolin, kaempferol, diosmetin, sinensetin, and nobiletin were 0.8 - 0.9 mg / kg; thus, in Examples 1-6, the contents of flavonoids and polyphenols in the roots increased significantly after grafting. In Comparative Examples 1-3, the contents of eriodictyol glycoside and dihydroquercetin in the roots of the budstock seedlings before and after grafting were both about 0.4 mg / kg, and the contents of components such as hesperetin, naringin, neohesperidin, rhoifolin, naringenin, luteolin, kaempferol, diosmetin, sinensetin, and nobiletin were both about 0.2 mg / kg; specifically, the detection results of the root component contents of the budstock seedlings before and after grafting in Example 1 are shown in the appendix Figures 1 - 4 as follows. Furthermore, comparing the field planting situations in Examples 1-6 and Comparative Examples 1-3, the field planting time was half a year, and the soil fertility, temperature, humidity, fertilization, and watering conditions of the planting sites were the same. Observing the growth of Macadamia integrifolia grafted seedlings, the seedling height of the Macadamia integrifolia grafted seedlings in Examples 1-3 was about 30% higher than that in Comparative Examples 1-3, and the stem diameter of the Macadamia integrifolia grafted seedlings in Examples 1-3 was about 20% thicker than that in Comparative Examples 1-3. Thus, it can be seen that using the method of the present invention for seedling raising, the subsequent growth of the seedlings is better and the seedling quality is high.

[0137] Using hydroponics to add AlCl 3 to simulate the acid-aluminum toxicity of the soil, AlCl 3The addition amount is 50 g / L. Acid-aluminum tolerance tests were carried out on the macadamia nut grafted seedlings in Examples 1-6 and Comparative Examples 1-3. The acid-aluminum tolerance of the macadamia nut grafted seedlings was judged according to whether the root growth and the growth of the above-ground part of the macadamia nut grafted seedlings were inhibited. The root growth and the growth of the above-ground part of the macadamia nut grafted seedlings in Examples 1-4 were basically not affected, while the growth of the above-ground part of the macadamia nut grafted seedlings in Examples 5-6 and Comparative Examples 1-3 was inhibited to a certain extent, and the inhibition of the roots was particularly severe.

[0138] From the above test results, it can be seen that for a method of improving the grafting survival rate of seedlings and promoting well-developed roots in the present invention, before the budstock seedlings are grafted and before the grafting buds are taken from the improved variety mother trees, they are pretreated with the budstock seedling pretreatment solution and the improved variety mother tree pretreatment solution respectively. Compound sodium nitrophenolate is added as a powerful cell activator in the pretreatment solution. After contacting with the budstock seedlings and the improved variety mother trees, it can quickly penetrate into the plant body, promote the protoplasm flow of cells, improve cell vitality, help absorb potassium and molybdenum in the pretreatment solution and major and trace elements in the soil, and effectively improve the grafting survival rate of macadamia nut seedlings; apply a grafting healing agent around the grafting incision. Erythromycin ointment and sulfur powder in the grafting healing agent are used to prevent bacterial infection in the air. Added indoleacetic acid and gibberellin are used as exogenous hormones to stimulate the cells near the grafting incision to accelerate division and accelerate the incision healing. The amino acid trace element chelate effectively supplements elements such as iron, iron, magnesium, and zinc, promotes the growth of callus, and effectively improves the grafting survival rate of macadamia nut seedlings; apply a root-promoting fertilizer to the roots after one month of grafting, continuously supplement organic matter, amino acids, potassium, iron, iron, magnesium, zinc and other elements, effectively promote the root growth of the budstock seedlings, greatly increase the content of flavonoids and polyphenols in the roots of the budstock seedlings, effectively improve the grafting survival rate of macadamia nut seedlings, and at the same time effectively promote the well-developed roots of macadamia nut seedlings, thereby effectively driving the healthy growth of macadamia nut grafted seedlings; when using the method of the present invention for seedling cultivation, the seedling age is smaller, the survival rate is more than 95%, the seedling emergence time is short, and the seedlings can be outplanted after six months of cultivation after grafting. The outplanted seedlings have well-developed roots, and the content of various flavonoid components and polyphenol components in the roots has increased significantly. When the macadamia nut grafted seedlings are planted in the field later, they grow vigorously and have good growth; the budstock seedling pretreatment solution also includes manganese sulfate to supplement manganese element, which can regulate the oxidation-reduction reaction in the budstock seedlings, is beneficial to the synthesis of amino acids and proteins, promotes root growth, makes the root and stem of the budstock seedlings thicker, promotes the well-developed roots of macadamia nut seedlings, and at the same time improves the grafting survival rate of macadamia nut seedlings; apply a mixture of peat soil to the roots. The cooperation of peat soil, zeolite powder and edible mushroom residue can loosen the soil texture and at the same time increase the organic matter content of the soil, which can effectively promote the root growth of the budstock seedlings; peat soil is slightly acidic, and adding orange peel water with weak acidity can not only supplement the nutrient elements required for the growth of the budstock seedlings, but also effectively remove insects, and at the same time can increase the acid-aluminum tolerance of the budstock seedlings.

[0139] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it based on the present invention, which will be obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection required by the present invention.

Claims

1. A method for improving the grafting survival rate of seedlings and promoting well-developed roots, characterized in that, it includes the following steps: (1) Selecting rootstock seedlings: Select healthy Macadamia integrifolia seedlings with a stem diameter of 0.3 cm to 1.2 cm as rootstock seedlings; (2) Pretreatment of rootstock seedlings: 5 to 7 days before grafting, apply 80 to 100 g / plant of the pretreatment solution for rootstock seedlings to the roots of the rootstock seedlings; the pretreatment solution for rootstock seedlings, calculated by weight, includes: 1000 parts of biogas slurry, 0.2 to 0.3 parts of 1.8 wt% sodium nitrophenolate aqueous solution, 3 to 5 parts of sorbitol boron, 35 to 38 parts of potassium dihydrogen phosphate, 5 to 7 parts of ammonium molybdate; (3) Pretreatment of elite mother trees and selection of grafting buds: Select elite Macadamia integrifolia mother trees without diseases and pests. 3 to 5 days before grafting, spray the pretreatment solution for elite mother trees on the whole tree, and then select non-lignified tender buds as grafting buds; the pretreatment solution for elite mother trees is obtained by diluting the pretreatment solution for rootstock seedlings by 10 to 15 times; (4) Grafting: Graft the grafting buds onto the rootstock seedlings described in step (2), apply a grafting healing agent around the grafting incision, and then wrap and fix the grafting incision with a clean plastic film to obtain Macadamia integrifolia grafted seedlings; the grafting healing agent, calculated by weight, includes: 1000 parts of erythromycin ointment, 80 to 90 parts of sulfur powder, 6 to 8 parts of amino acid trace element chelate, 0.1 to 0.2 parts of indoleacetic acid, 0.2 to 0.3 parts of gibberellin; The amino acid trace element chelate, calculated by weight, includes: 45 to 50 parts of amino acid chelated iron, 35 to 40 parts of amino acid chelated copper, 25 to 30 parts of amino acid chelated magnesium, 15 to 20 parts of amino acid chelated zinc; (5) Management of grafted seedlings: 5.1) Light management: Build a shading net with a shading degree of 60% to 65% above the seedbed of Macadamia integrifolia grafted seedlings; 5.2) Temperature and humidity control: Cover a layer of sawdust powder with a thickness of 4 to 5 cm on the seedbed of Macadamia integrifolia grafted seedlings, control the temperature of the seedbed of Macadamia integrifolia grafted seedlings at 18 to 26 °C, and control the humidity at 85% to 90%; 5.3) Fertilization: Apply a root-promoting fertilizer to the roots after one month of grafting, and the fertilization depth is 10 to 15 cm; the root-promoting fertilizer, calculated by weight, includes: 100 to 120 parts of decomposed livestock manure, 10 to 13 parts of agricultural amino acid raw powder, 8 to 10 parts of potassium dihydrogen phosphate, 3 to 5 parts of amino acid trace element chelate, 0.1 to 0.2 parts of acephate.

2. A method for improving the grafting survival rate of seedlings and promoting well-developed roots according to claim 1, characterized in that, 6 to 7 days before grafting in step (2), apply 90 to 100 g / plant of the pretreatment solution for rootstock seedlings to the roots of the rootstock seedlings; the pretreatment solution for rootstock seedlings, calculated by weight, includes: 1000 parts of biogas slurry, 0.25 to 0.3 parts of 1.8 wt% sodium nitrophenolate aqueous solution, 4 to 5 parts of sorbitol boron, 36 to 38 parts of potassium dihydrogen phosphate, 6 to 7 parts of ammonium molybdate.

3. A method for improving the grafting survival rate of seedlings and promoting well-developed roots according to claim 1, characterized in that, the pretreatment solution for rootstock seedlings further includes 4 to 6 parts by weight of manganese sulfate.

4. A method for improving the grafting survival rate of seedlings and promoting well-developed roots according to claim 1, characterized in that, in step (3), select disease- and pest-free excellent Macadamia integrifolia mother trees. Four to five days before grafting, spray the pretreatment liquid for excellent mother trees on the whole tree to make the leaves and stems of the excellent Macadamia integrifolia mother trees fully moist, and then select non-lignified tender buds as grafting buds; the pretreatment liquid for excellent mother trees is obtained by diluting the pretreatment liquid for budded rootstocks by 12 to 15 times.

5. A method for improving the grafting survival rate of seedlings and promoting well-developed roots according to claim 1, characterized in that, the grafting healing agent, by weight, comprises: 1000 parts of erythromycin ointment, 85 to 90 parts of sulfur powder, 7 to 8 parts of amino acid trace element chelate, 0.15 to 0.2 part of indoleacetic acid, 0.25 to 0.3 part of gibberellin; the amino acid trace element chelate, by weight, comprises: 47 to 50 parts of amino acid chelated iron, 36 to 40 parts of amino acid chelated copper, 28 to 30 parts of amino acid chelated magnesium, 18 to 20 parts of amino acid chelated zinc.

6. A method for improving the grafting survival rate of seedlings and promoting well-developed roots according to claim 1, characterized in that, in step 5.2), cover a layer of sawdust powder with a thickness of 4.5 to 5 cm on the seedling bed of grafted Macadamia integrifolia, control the temperature of the seedling bed of grafted Macadamia integrifolia at 20 to 26 °C, and control the humidity at 87 to 90%.

7. A method for improving the grafting survival rate of seedlings and promoting well-developed roots according to claim 1, characterized in that, in step 5.3), apply 120 to 150 g / plant of root-promoting fertilizer.

8. A method for improving the grafting survival rate of seedlings and promoting well-developed roots according to claim 1, characterized in that, the root-promoting fertilizer in step 5.3), by weight, comprises: 110 to 120 parts of decomposed livestock and poultry manure, 11 to 13 parts of agricultural amino acid raw powder, 9 to 10 parts of potassium dihydrogen phosphate, 4 to 5 parts of amino acid trace element chelate, 0.15 to 0.2 part of acephate.

9. A method for improving the grafting survival rate of seedlings and promoting well-developed roots according to claim 1, characterized in that, 10 to 15 days after applying the root-promoting fertilizer in step 5.3), apply a peat soil mixture by root application, and the root application depth is 5 to 8 cm; the peat soil mixture, by weight, comprises: 100 parts of peat soil, 25 to 30 parts of zeolite powder, 30 to 35 parts of edible mushroom residue; the dosage of the peat soil mixture is 350 to 400 g / plant.

10. A method for improving the grafting survival rate of seedlings and promoting well-developed roots according to claim 9, characterized in that, after applying the peat soil mixture by root application in step 5.3), apply orange peel water by root application.

Images