A thrips attractant and a method for preparing an attractant core using the same as a core material and a trapping device

Abstract

The present invention discloses a thrips attractant, which is prepared by mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of (1-20):(1-20):(1-10):(1-20):(1-20). The present invention also discloses a method for preparing an attractant core using the thrips attractant as a core material, first dissolving the thrips attractant in n-hexane, and then slowly releasing it in a carrier, to obtain. The present invention also discloses a thrips combined trapping device, which is obtained by hanging an attractant core prepared with the thrips attractant core material on a blue sticky insect board or a yellow sticky insect board. The thrips attractant of the present invention has the advantages of simple use method, stable product performance, multiple attractant types and environmental protection, greatly reduces the use of chemical pesticides, effectively controls the harm of thrips pests to vegetables, fruits or flowers, and opens up new ideas for the comprehensive prevention and control technology of thrips pests.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural pest control, and more particularly to a thrips attractant, a method for preparing an attractant core using the thrips attractant as a core material, and a trapping device. Background Art

[0002] Thrips is a general term for insects of the order Thysanoptera. They are tiny and often hide in flowers and young leaves to cause harm. At present, more than 7,700 species of thrips have been recorded worldwide, with more than 570 species in China (Majid et al., 2011; Mound et al., 2014). So far, more than 2,000 species of Thripidae have been recorded worldwide, belonging to about 276 genera in 4 subfamilies, with 79 genera and 315 species in China (Hu Qingling, 2013). Thrips have strong reproductive capacity, serious generation overlap, and high frequency of outbreaks. Global trade and climate warming have accelerated the spread and damage of thrips pests. Thrips pests have gradually become one of the most important agricultural pest groups in the world. They can spread a variety of viruses including the thrips virus, and are also important invasive alien pests that cause disasters.

[0003] Studies have found that insect pheromones, as a non-chemical control measure, can not only be used to monitor pest population dynamics, but can also be combined with other prevention and control measures to improve pest control effectiveness, which is beneficial to delaying the evolution of insect resistance to chemical pesticides. At the same time, it can also protect natural enemy species and their population sizes in nature, which is beneficial to the sustainable management of agricultural pests.

[0004] In recent years, patents related to thrips attraction have also appeared one after another. For example, the invention patent with application number 201510806166.7 discloses a method for controlling sugarcane thrips, in which a glue bait made of cyantraniliprole, attractant and cyclodextrin is evenly spread on the heart leaves of sugarcane, but it causes pollution to the environment. The invention patent with application number 201410604939.9 discloses an agent for attracting western flower thrips, including (R)-lavender acetate, and styryl (S)-2-methylbutyrate or an analog of styryl (S)-2-methylbutyrate. The invention patent with application number 201310417293.9 discloses an attractant for thrips, which is composed of methyl anthranilate, geraniol, plant essence and alcohol mixture.

[0005] At the same time, there are also related patents that place the pheromone attractant core of thrips insects on an insect trap made of sticky glue and a substrate. For example, the invention patent with application number 200910227375.0 discloses a pure natural plant insecticide made from natural plants to control thrips pests, and the invention patents with application numbers 200820008447.3, 200420027355.1 and 201020020566.8 all design sticky insect traps based on the thrips' tendency to color to trap and kill pests. However, relying on color to trap and kill thrips has the problem of insufficient attraction and poor effect.

[0006] In addition, due to the wide variety of thrips species, the attractant composed of chemical components disclosed in the invention patent with application number 201110101489.8 is only effective against some thrips, but has poor effects on other thrips.

[0007] In summary, there are currently no effective green control measures against thrips, and the damage is still very serious.

[0008] Therefore, how to use attractant pheromones to effectively and comprehensively control thrips is an urgent problem that technicians in this field need to solve. Summary of the invention

[0009] In view of this, the object of the present invention is to provide a thrips attractant and a method for preparing an attractant core using the thrips attractant as a core material and a trapping device, so as to solve the deficiencies in the prior art.

[0010] In order to achieve the above object, the present invention adopts the following technical solution:

[0011] A thrips attractant is prepared by mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of (1-20):(1-20):(1-10):(1-20):(1-20).

[0012] Furthermore, the above-mentioned thrips attractant is prepared by mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of (1-10):(1-10):(1-5):(1-10):(1-10).

[0013] Furthermore, the above-mentioned thrips attractant is prepared by mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:5:5.

[0014] A further beneficial effect of the above is that methyl benzoate and phenylacetaldehyde are aroma substances, which have a good attracting effect on thrips pests such as yellow-breasted thrips, tobacco thrips, New Zealand flower thistle, western flower thrips and color thrips.

[0015] 3-Hydroxybenzaldehyde is a benzene ring substance and also an aroma substance. It has a certain attracting effect on flower thrips and common giant thrips.

[0016] Cis-3-hexenal is a green leaf odor and a host volatile with a high content. It has a certain attraction to western flower thrips and has a certain synergistic effect on methyl benzoate and 3-hydroxybenzaldehyde.

[0017] Geranyl linalool belongs to the terpene class and has a good attracting effect on thrips pests such as western flower thrips, palm thrips, flower thrips, common giant thrips and yellow-breasted thrips.

[0018] A method for preparing an attractant core I using the above-mentioned thrips attractant as a core material, firstly mixing the thrips attractant, an amphiphilic block copolymer and deionized water in a mass ratio of (1-5):(5-25):(5-10) to prepare micelles, and then loading the mixture into a 5mL centrifuge tube to obtain an attractant core I.

[0019] Further, the method specifically includes the following steps:

[0020] (1) dissolving a thrips attractant in n-hexane to obtain a thrips attractant solution;

[0021] (2) mixing the thrips attractant solution with a DMF (N,N-dimethylformamide) solution of the amphiphilic block copolymer to obtain a mixed solution;

[0022] (3) adding deionized water dropwise to the mixed solution under stirring conditions, stirring, and obtaining micelles;

[0023] (4) The micelles were placed into a 5 mL centrifuge tube with a cap having several air holes with a diameter of 0.1-0.6 mm to obtain attractant core I.

[0024] A further beneficial effect of adopting the above method is that the amphiphilic block copolymer (MPEG-PCL) selected in the present invention has good biocompatibility, excellent drug permeability for small molecule drugs, strong drug loading capacity and good stability, is a biodegradable and environmentally friendly material, and in the preparation process, the amount of organic solvent used is small, thereby avoiding environmental pollution during use.

[0025] The invention prepares micelles by mixing a thrips attractant with an amphiphilic block copolymer. After the thrips attractant acts on the lipophilic end of the copolymer, the hydrophilic end self-assembles with water to form a micelle with a core-shell structure having a hydrophobic core and a hydrophilic shell. The lipophilic end can make the fat-soluble sex pheromone more easily encapsulated, thereby increasing the encapsulation rate of the sex pheromone by 20%. The thrips attractant of the invention is slowly released through the pores of the wall material, thereby achieving a long-term and stable release effect.

[0026] A method for preparing an attractant core II using the above-mentioned thrips attractant as a core material, firstly loading multi-walled carbon nanotubes into a 5mL centrifuge tube, then adding the thrips attractant, the mass ratio of the thrips attractant to the multi-walled carbon nanotubes being 1:(10-20), mixing and oscillating for 12-24 hours, and then obtaining the attractant core II.

[0027] Further, the method specifically includes the following steps:

[0028] (1) dissolving a thrips attractant in n-hexane to obtain a thrips attractant solution;

[0029] (2) placing the multi-walled carbon nanotubes into a 5 mL centrifuge tube, slowly adding the thrips attractant solution, and mixing well to obtain a mixture;

[0030] (3) Place the mixture on a constant temperature oscillator and oscillate at 20-25°C and 100-150 r / min for 12-24 hours to obtain attractant core II.

[0031] The above further beneficial effect is that, compared with conventional rubber heads and capillary attractants, the unique hollow structure of the multi-walled carbon nanotubes selected by the present invention has a larger specific surface area and a higher loading capacity. The present invention can not only make the loading capacity of the thrips attractant larger by loading the thrips attractant into the multi-walled carbon nanotubes, but also realize the slow release of the thrips attractant in the microcapsule, achieve a long-acting, sustained-release effect, and improve storage stability. Moreover, the preparation process of the microcapsule of the present invention is simple and easy, and the amount of organic solvent used in the preparation process is small, which can avoid environmental pollution during use, and is an efficient and environmentally friendly material.

[0032] A method for preparing an attractant core III using the above thrips attractant as a core material comprises first dissolving the thrips attractant in n-hexane and then adding the thrips attractant into a rubber carrier to obtain the attractant core III.

[0033] A further beneficial effect of adopting the above method is that:

[0034] A combined trapping and killing device for thrips contains the trap core I made by the above preparation method, or contains the trap core II made by the above preparation method, or contains the trap core III made by the above preparation method.

[0035] A method for preparing the above-mentioned combined trapping and killing device for thrips is provided. The trap core I prepared by the above-mentioned preparation method is hung on a blue sticky insect board or a yellow sticky insect board, or the trap core II prepared by the above-mentioned preparation method is hung on a blue sticky insect board or a yellow sticky insect board, or the trap core III prepared by the above-mentioned preparation method is hung on a blue sticky insect board or a yellow sticky insect board, so as to obtain the combined trapping and killing device for thrips.

[0036] In the combined trapping and killing device for thrips of the present invention, the blue sticky insect board and the yellow sticky insect board have the following characteristics: 1. The principle is to kill individual insects by reacting to the blue or yellow color and pheromones, so no resistance is produced, it is green and environmentally friendly, does not pollute the environment, and is the preferred product of green agriculture; 2. The specific color is adopted, and the pheromone-attracting effect on specific insects is remarkable, which can effectively reduce the insect population density, reduce the use of medicines, reduce costs, solve the problem of pesticide residues, and improve the ecological environment; 3. Imported high-viscosity waterproof glue is adopted, which does not flow at high temperatures, is resistant to sunlight and rain, is durable, is coated on both sides, and the double-sided trapping is effective for more than half a year; 4. The green barriers to international agricultural product trade are broken, the quality and safety of agricultural products and prices are improved, and agricultural income is increased; 5. The operation is convenient, it can be used after opening, and time and labor are saved.

[0037] It can be seen from the above technical solution that compared with the prior art, the beneficial effects of the present invention are as follows:

[0038] 1. The present invention uses methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde as raw materials for compounding, and the prepared thrips attractant has high efficacy and long lasting effect; after the thrips attractant is made into an attractant core, it is combined with a blue sticky insect board or a yellow sticky insect board to prepare a combined trapping device for trapping and killing a variety of thrips pests, and has a similar control effect to that of applying pesticides alone, and the control effect is improved by 3 to 5 times compared with the control effect of using blue sticky insect boards or yellow sticky insect boards alone.

[0039] 2. The thrips attractant of the present invention can attract a variety of thrips pests such as western flower thrips, palm thrips, flower thrips, yellow-breasted thrips, tobacco thrips and bean thrips, and has the advantages of simple use, stable product performance, multiple types of attractants and environmental protection. It greatly reduces the use of chemical pesticides, effectively controls the harm of thrips pests to vegetables, fruits or flowers, improves the green quality of agricultural products such as fruits and vegetables, is conducive to promoting the sustainable development of green products, and opens up new ideas for the comprehensive prevention and control technology of thrips pests.

[0040] 3. The thrips attractant of the present invention is not used in combination with pesticides, but it has achieved a control effect similar to that of pesticides, realizing a new breakthrough in the green control of thrips. In the later stage, the application dosage and method of use will be adapted to local conditions, and it is expected to be widely promoted and applied. DETAILED DESCRIPTION

[0041] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0042] Example 1

[0043] 1. The preparation method of the thrips combined trapping device I specifically comprises the following steps:

[0044] (1) Methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde are mixed in a mass ratio of 5:3:2:6:4 to obtain a thrips attractant;

[0045] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0046] (3) mixing the thrips attractant solution with the DMF solution of the amphiphilic block copolymer to obtain a mixed solution;

[0047] (4) under stirring conditions, adding deionized water dropwise to the mixed solution, stirring, and obtaining micelles; wherein the mass ratio of the thrips attractant, the amphiphilic block copolymer, and the deionized water is 3:20:7;

[0048] (5) The micelles were placed in a 5 mL centrifuge tube with a cover having several 0.4 mm diameter air holes to obtain attractant core I;

[0049] (6) Hang the bait core I on the blue sticky insect board to obtain the thrips combined trapping device I.

[0050] 2. The preparation method of the thrips combined trapping device II specifically comprises the following steps:

[0051] (1) Methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde are mixed in a mass ratio of 5:3:2:6:4 to obtain a thrips attractant;

[0052] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0053] (3) placing the multi-walled carbon nanotubes into a 5 mL centrifuge tube, slowly adding the thrips attractant solution, and mixing well to obtain a mixture; wherein the mass ratio of the thrips attractant to the multi-walled carbon nanotubes is 1:15;

[0054] (4) The mixture was placed on a constant temperature oscillator and oscillated at 22°C and 120 r / min for 18 h to obtain the bait core II;

[0055] (5) Hang the bait core II on the blue sticky insect board to obtain the thrips combined trapping device II.

[0056] 3. The preparation method of the thrips combined trapping device III specifically comprises the following steps:

[0057] (1) Methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde are mixed in a mass ratio of 5:3:2:6:4 to obtain a thrips attractant;

[0058] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0059] (3) adding the thrips attractant solution into the rubber carrier, and after the n-hexane is completely volatilized, the attractant core III is obtained;

[0060] (5) Hang the bait core III on the blue sticky insect board to obtain the thrips combined trapping device III.

[0061] Example 2

[0062] 1. The preparation method of the thrips combined trapping device I specifically comprises the following steps:

[0063] (1) Methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde are mixed in a mass ratio of 5:3:2:6:4 to obtain a thrips attractant;

[0064] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0065] (3) mixing the thrips attractant solution with the DMF solution of the amphiphilic block copolymer to obtain a mixed solution;

[0066] (4) under stirring conditions, adding deionized water dropwise to the mixed solution, stirring, and obtaining micelles; wherein the mass ratio of the thrips attractant, the amphiphilic block copolymer, and the deionized water is 3:20:7;

[0067] (5) The micelles were placed in a 5 mL centrifuge tube with a cover having several 0.4 mm diameter air holes to obtain attractant core I;

[0068] (6) Hang the bait core I on the yellow sticky insect board to obtain the thrips combined trapping device I.

[0069] 2. The preparation method of the thrips combined trapping device II specifically comprises the following steps:

[0070] (1) Methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde are mixed in a mass ratio of 5:3:2:6:4 to obtain a thrips attractant;

[0071] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0072] (3) placing the multi-walled carbon nanotubes into a 5 mL centrifuge tube, slowly adding the thrips attractant solution, and mixing well to obtain a mixture; wherein the mass ratio of the thrips attractant to the multi-walled carbon nanotubes is 1:15;

[0073] (4) The mixture was placed on a constant temperature oscillator and oscillated at 22°C and 120 r / min for 18 h to obtain the bait core II;

[0074] (5) Hang the bait core II on the yellow sticky insect board to obtain the thrips combined trapping device II.

[0075] 3. The preparation method of the thrips combined trapping device III specifically comprises the following steps:

[0076] (1) Methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde are mixed in a mass ratio of 5:3:2:6:4 to obtain a thrips attractant;

[0077] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0078] (3) adding the thrips attractant solution into the rubber carrier, and after the n-hexane is completely volatilized, the attractant core III is obtained;

[0079] (5) Hang the bait core III on the yellow sticky insect board to obtain the thrips combined trapping device III.

[0080] Example 3

[0081] 1. The preparation method of the thrips combined trapping device I specifically comprises the following steps:

[0082] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:5:5 to obtain a thrips attractant;

[0083] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0084] (3) mixing the thrips attractant solution with the DMF solution of the amphiphilic block copolymer to obtain a mixed solution;

[0085] (4) under stirring conditions, adding deionized water dropwise to the mixed solution, stirring, and obtaining micelles; wherein the mass ratio of the thrips attractant, the amphiphilic block copolymer, and the deionized water is 3:20:7;

[0086] (5) The micelles were placed in a 5 mL centrifuge tube with a cover having several 0.4 mm diameter air holes to obtain attractant core I;

[0087] (6) Hang the bait core I on the blue sticky insect board to obtain the thrips combined trapping device I.

[0088] 2. The preparation method of the thrips combined trapping device II specifically comprises the following steps:

[0089] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:5:5 to obtain a thrips attractant;

[0090] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0091] (3) placing the multi-walled carbon nanotubes into a 5 mL centrifuge tube, slowly adding the thrips attractant solution, and mixing well to obtain a mixture; wherein the mass ratio of the thrips attractant to the multi-walled carbon nanotubes is 1:15;

[0092] (4) The mixture was placed on a constant temperature oscillator and oscillated at 22°C and 120 r / min for 18 h to obtain the bait core II;

[0093] (5) Hang the bait core II on the blue sticky insect board to obtain the thrips combined trapping device II.

[0094] 3. The preparation method of the thrips combined trapping device III specifically comprises the following steps:

[0095] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:5:5 to obtain a thrips attractant;

[0096] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0097] (3) adding the thrips attractant solution into the rubber carrier, and after the n-hexane is completely volatilized, the attractant core III is obtained;

[0098] (5) Hang the bait core III on the blue sticky insect board to obtain the thrips combined trapping device III.

[0099] Example 4

[0100] 1. The preparation method of the thrips combined trapping device I specifically comprises the following steps:

[0101] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:5:5 to obtain a thrips attractant;

[0102] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0103] (3) mixing the thrips attractant solution with the DMF solution of the amphiphilic block copolymer to obtain a mixed solution;

[0104] (4) under stirring conditions, adding deionized water dropwise to the mixed solution, stirring, and obtaining micelles; wherein the mass ratio of the thrips attractant, the amphiphilic block copolymer, and the deionized water is 3:20:7;

[0105] (5) The micelles were placed in a 5 mL centrifuge tube with a cover having several 0.4 mm diameter air holes to obtain attractant core I;

[0106] (6) Hang the bait core I on the yellow sticky insect board to obtain the thrips combined trapping device I.

[0107] 2. The preparation method of the thrips combined trapping device II specifically comprises the following steps:

[0108] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:5:5 to obtain a thrips attractant;

[0109] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0110] (3) placing the multi-walled carbon nanotubes into a 5 mL centrifuge tube, slowly adding the thrips attractant solution, and mixing well to obtain a mixture; wherein the mass ratio of the thrips attractant to the multi-walled carbon nanotubes is 1:15;

[0111] (4) The mixture was placed on a constant temperature oscillator and oscillated at 22°C and 120 r / min for 18 h to obtain the bait core II;

[0112] (5) Hang the bait core II on the yellow sticky insect board to obtain the thrips combined trapping device II.

[0113] 3. The preparation method of the thrips combined trapping device III specifically comprises the following steps:

[0114] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:5:5 to obtain a thrips attractant;

[0115] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0116] (3) adding the thrips attractant solution into the rubber carrier, and after the n-hexane is completely volatilized, the attractant core III is obtained;

[0117] (5) Hang the bait core III on the yellow sticky insect board to obtain the thrips combined trapping device III.

[0118] Example 5

[0119] 1. The preparation method of the thrips combined trapping device I specifically comprises the following steps:

[0120] (1) methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde are mixed in a mass ratio of 6:5:1:3:5 to obtain a thrips attractant;

[0121] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0122] (3) mixing the thrips attractant solution with the DMF solution of the amphiphilic block copolymer to obtain a mixed solution;

[0123] (4) under stirring conditions, adding deionized water dropwise to the mixed solution, stirring, and obtaining micelles; wherein the mass ratio of the thrips attractant, the amphiphilic block copolymer, and the deionized water is 3:20:7;

[0124] (5) The micelles were placed in a 5 mL centrifuge tube with a cover having several 0.4 mm diameter air holes to obtain attractant core I;

[0125] (6) Hang the bait core I on the blue sticky insect board to obtain the thrips combined trapping device I.

[0126] 2. The preparation method of the thrips combined trapping device II specifically comprises the following steps:

[0127] (1) methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde are mixed in a mass ratio of 6:5:1:3:5 to obtain a thrips attractant;

[0128] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0129] (3) placing the multi-walled carbon nanotubes into a 5 mL centrifuge tube, slowly adding the thrips attractant solution, and mixing well to obtain a mixture; wherein the mass ratio of the thrips attractant to the multi-walled carbon nanotubes is 1:15;

[0130] (4) The mixture was placed on a constant temperature oscillator and oscillated at 22°C and 120 r / min for 18 h to obtain the bait core II;

[0131] (5) Hang the bait core II on the blue sticky insect board to obtain the thrips combined trapping device II.

[0132] 3. The preparation method of the thrips combined trapping device III specifically comprises the following steps:

[0133] (1) methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde are mixed in a mass ratio of 6:5:1:3:5 to obtain a thrips attractant;

[0134] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0135] (3) adding the thrips attractant solution into the rubber carrier, and after the n-hexane is completely volatilized, the attractant core III is obtained;

[0136] (5) Hang the bait core III on the blue sticky insect board to obtain the thrips combined trapping device III.

[0137] Example 6

[0138] 1. The preparation method of the thrips combined trapping device I specifically comprises the following steps:

[0139] (1) methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde are mixed in a mass ratio of 6:5:1:3:5 to obtain a thrips attractant;

[0140] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0141] (3) mixing the thrips attractant solution with the DMF solution of the amphiphilic block copolymer to obtain a mixed solution;

[0142] (4) under stirring conditions, adding deionized water dropwise to the mixed solution, stirring, and obtaining micelles; wherein the mass ratio of the thrips attractant, the amphiphilic block copolymer, and the deionized water is 3:20:7;

[0143] (5) The micelles were placed in a 5 mL centrifuge tube with a cover having several 0.4 mm diameter air holes to obtain attractant core I;

[0144] (6) Hang the bait core I on the yellow sticky insect board to obtain the thrips combined trapping device I.

[0145] 2. The preparation method of the thrips combined trapping device II specifically comprises the following steps:

[0146] (1) methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde are mixed in a mass ratio of 6:5:1:3:5 to obtain a thrips attractant;

[0147] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0148] (3) placing the multi-walled carbon nanotubes into a 5 mL centrifuge tube, slowly adding the thrips attractant solution, and mixing well to obtain a mixture; wherein the mass ratio of the thrips attractant to the multi-walled carbon nanotubes is 1:15;

[0149] (4) The mixture was placed on a constant temperature oscillator and oscillated at 22°C and 120 r / min for 18 h to obtain the bait core II;

[0150] (5) Hang the bait core II on the yellow sticky insect board to obtain the thrips combined trapping device II.

[0151] 3. The preparation method of the thrips combined trapping device III specifically comprises the following steps:

[0152] (1) methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde are mixed in a mass ratio of 6:5:1:3:5 to obtain a thrips attractant;

[0153] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0154] (3) adding the thrips attractant solution into the rubber carrier, and after the n-hexane is completely volatilized, the attractant core III is obtained;

[0155] (5) Hang the bait core III on the yellow sticky insect board to obtain the thrips combined trapping device III.

[0156] Example 7

[0157] 1. The preparation method of the thrips combined trapping device I specifically comprises the following steps:

[0158] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 6:3:1:5:5 to obtain a thrips attractant;

[0159] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0160] (3) mixing the thrips attractant solution with the DMF solution of the amphiphilic block copolymer to obtain a mixed solution;

[0161] (4) under stirring conditions, adding deionized water dropwise to the mixed solution, stirring, and obtaining micelles; wherein the mass ratio of the thrips attractant, the amphiphilic block copolymer, and the deionized water is 3:20:7;

[0162] (5) The micelles were placed in a 5 mL centrifuge tube with a cover having several 0.4 mm diameter air holes to obtain attractant core I;

[0163] (6) Hang the bait core I on the blue sticky insect board to obtain the thrips combined trapping device I.

[0164] 2. The preparation method of the thrips combined trapping device II specifically comprises the following steps:

[0165] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 6:3:1:5:5 to obtain a thrips attractant;

[0166] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0167] (3) placing the multi-walled carbon nanotubes into a 5 mL centrifuge tube, slowly adding the thrips attractant solution, and mixing well to obtain a mixture; wherein the mass ratio of the thrips attractant to the multi-walled carbon nanotubes is 1:15;

[0168] (4) The mixture was placed on a constant temperature oscillator and oscillated at 22°C and 120 r / min for 18 h to obtain the bait core II;

[0169] (5) Hang the bait core II on the blue sticky insect board to obtain the thrips combined trapping device II.

[0170] 3. The preparation method of the thrips combined trapping device III specifically comprises the following steps:

[0171] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 6:3:1:5:5 to obtain a thrips attractant;

[0172] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0173] (3) adding the thrips attractant solution into the rubber carrier, and after the n-hexane is completely volatilized, the attractant core III is obtained;

[0174] (5) Hang the bait core III on the blue sticky insect board to obtain the thrips combined trapping device III.

[0175] Example 8

[0176] 1. The preparation method of the thrips combined trapping device I specifically comprises the following steps:

[0177] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 6:3:1:5:5 to obtain a thrips attractant;

[0178] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0179] (3) mixing the thrips attractant solution with the DMF solution of the amphiphilic block copolymer to obtain a mixed solution;

[0180] (4) under stirring conditions, adding deionized water dropwise to the mixed solution, stirring, and obtaining micelles; wherein the mass ratio of the thrips attractant, the amphiphilic block copolymer, and the deionized water is 3:20:7;

[0181] (5) The micelles were placed in a 5 mL centrifuge tube with a cover having several 0.4 mm diameter air holes to obtain attractant core I;

[0182] (6) Hang the bait core I on the yellow sticky insect board to obtain the thrips combined trapping device I.

[0183] 2. The preparation method of the thrips combined trapping device II specifically comprises the following steps:

[0184] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 6:3:1:5:5 to obtain a thrips attractant;

[0185] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0186] (3) placing the multi-walled carbon nanotubes into a 5 mL centrifuge tube, slowly adding the thrips attractant solution, and mixing well to obtain a mixture; wherein the mass ratio of the thrips attractant to the multi-walled carbon nanotubes is 1:15;

[0187] (4) The mixture was placed on a constant temperature oscillator and oscillated at 22°C and 120 r / min for 18 h to obtain the bait core II;

[0188] (5) Hang the bait core II on the yellow sticky insect board to obtain the thrips combined trapping device II.

[0189] 3. The preparation method of the thrips combined trapping device III specifically comprises the following steps:

[0190] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 6:3:1:5:5 to obtain a thrips attractant;

[0191] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0192] (3) adding the thrips attractant solution into the rubber carrier, and after the n-hexane is completely volatilized, the attractant core III is obtained;

[0193] (5) Hang the bait core III on the yellow sticky insect board to obtain the thrips combined trapping device III.

[0194] Example 9

[0195] 1. The preparation method of the thrips combined trapping device I specifically comprises the following steps:

[0196] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:6:4 to obtain a thrips attractant;

[0197] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0198] (3) mixing the thrips attractant solution with the DMF solution of the amphiphilic block copolymer to obtain a mixed solution;

[0199] (4) under stirring conditions, adding deionized water dropwise to the mixed solution, stirring, and obtaining micelles; wherein the mass ratio of the thrips attractant, the amphiphilic block copolymer, and the deionized water is 3:20:7;

[0200] (5) The micelles were placed in a 5 mL centrifuge tube with a cover having several 0.4 mm diameter air holes to obtain attractant core I;

[0201] (6) Hang the bait core I on the blue sticky insect board to obtain the thrips combined trapping device I.

[0202] 2. The preparation method of the thrips combined trapping device II specifically comprises the following steps:

[0203] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:6:4 to obtain a thrips attractant;

[0204] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0205] (3) placing the multi-walled carbon nanotubes into a 5 mL centrifuge tube, slowly adding the thrips attractant solution, and mixing well to obtain a mixture; wherein the mass ratio of the thrips attractant to the multi-walled carbon nanotubes is 1:15;

[0206] (4) The mixture was placed on a constant temperature oscillator and oscillated at 22°C and 120 r / min for 18 h to obtain the bait core II;

[0207] (5) Hang the bait core II on the blue sticky insect board to obtain the thrips combined trapping device II.

[0208] 3. The preparation method of the thrips combined trapping device III specifically comprises the following steps:

[0209] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:6:4 to obtain a thrips attractant;

[0210] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0211] (3) adding the thrips attractant solution into the rubber carrier, and after the n-hexane is completely volatilized, the attractant core III is obtained;

[0212] (5) Hang the bait core III on the blue sticky insect board to obtain the thrips combined trapping device III.

[0213] Example 10

[0214] 1. The preparation method of the thrips combined trapping device I specifically comprises the following steps:

[0215] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:6:4 to obtain a thrips attractant;

[0216] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0217] (3) mixing the thrips attractant solution with the DMF solution of the amphiphilic block copolymer to obtain a mixed solution;

[0218] (4) under stirring conditions, adding deionized water dropwise to the mixed solution, stirring, and obtaining micelles; wherein the mass ratio of the thrips attractant, the amphiphilic block copolymer, and the deionized water is 3:20:7;

[0219] (5) The micelles were placed in a 5 mL centrifuge tube with a cover having several 0.4 mm diameter air holes to obtain attractant core I;

[0220] (6) Hang the bait core I on the yellow sticky insect board to obtain the thrips combined trapping device I.

[0221] 2. The preparation method of the thrips combined trapping device II specifically comprises the following steps:

[0222] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:6:4 to obtain a thrips attractant;

[0223] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0224] (3) placing the multi-walled carbon nanotubes into a 5 mL centrifuge tube, slowly adding the thrips attractant solution, and mixing well to obtain a mixture; wherein the mass ratio of the thrips attractant to the multi-walled carbon nanotubes is 1:15;

[0225] (4) The mixture was placed on a constant temperature oscillator and oscillated at 22°C and 120 r / min for 18 h to obtain the bait core II;

[0226] (5) Hang the bait core II on the yellow sticky insect board to obtain the thrips combined trapping device II.

[0227] 3. The preparation method of the thrips combined trapping device III specifically comprises the following steps:

[0228] (1) mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:6:4 to obtain a thrips attractant;

[0229] (2) dissolving the thrips attractant in n-hexane to obtain a thrips attractant solution;

[0230] (3) adding the thrips attractant solution into the rubber carrier, and after the n-hexane is completely volatilized, the attractant core III is obtained;

[0231] (5) Hang the bait core III on the yellow sticky insect board to obtain the thrips combined trapping device III.

[0232] Performance Testing

[0233] Take the thrips combined trapping device I (thrips attractant + amphiphilic block copolymer micelle + blue sticky insect board or yellow sticky insect board), thrips combined trapping device II (thrips attractant + multi-walled carbon nanotube microcapsule + blue sticky insect board or yellow sticky insect board) and thrips combined trapping device III (thrips attractant + rubber carrier + blue sticky insect board or yellow sticky insect board) prepared in Examples 1-10, and use a single blue sticky insect board as a control group 1 and a single yellow sticky insect board as a control group 2, and hang them in a strawberry greenhouse with thrips, 10 cm above the top of the plant, and the board surface is set perpendicular to the furrow surface. Count the data every 3 days, and count the total amount of thrips attracted for 15 days.

[0234] The test results of the mass ratio of thrips attractants in different groups and the total amount of 15-day attractants are shown in Table 1.

[0235] Table 1 The attracting effect of thrips attractants with different mass ratios on thrips in facility agriculture applications

[0236]

[0237]

[0238] As shown in Table 1, the thrips attractants prepared by the above five formulations of the present invention have an increase of 3 to 5 times in the amount of thrips attracted compared to the use of blue sticky insect boards or yellow sticky insect boards alone. Among them, Examples 3 and 4 (the mass ratio of methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool, and phenylacetaldehyde is 5:4:1:5:5) have the best thrips attracting effect, and the amount of thrips attracted by the blue sticky insect board is significantly higher than that of the yellow sticky insect board.

[0239] The above experiments show that the thrips attractant prepared by compounding methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde as raw materials has high efficacy and long lasting effect; the combined trapping device prepared by combining the attractant with a blue sticky insect board or a yellow sticky insect board after being made into an attractant core is used to trap and kill a variety of thrips pests, and has a similar control effect to that of applying pesticides alone, and is 3 to 5 times higher than the control effect of using blue sticky insect boards or yellow sticky insect boards alone.

[0240] At the same time, the present invention further verifies that the microcapsules of the amphiphilic block copolymer micelles and multi-walled carbon nanotubes have a good sustained-release effect as insect pheromone carriers.

[0241] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A thrips attractant, characterized in that: The product is prepared by mixing methyl benzoate, 3-hydroxybenzaldehyde, cis-3-hexenal, geranyl linalool and phenylacetaldehyde in a mass ratio of 5:4:1:5:

5.

2. A method for preparing an attractant core I using the thrips attractant according to claim 1 as a core material, characterized in that: The specific steps include: (1) dissolving a thrips attractant in n-hexane to obtain a thrips attractant solution; (2) mixing the thrips attractant solution with the DMF solution of the amphiphilic block copolymer to obtain a mixed solution; (3) adding deionized water dropwise to the mixed solution under stirring conditions, stirring, and obtaining micelles; Wherein, the mass ratio of the thrips attractant, the amphiphilic block copolymer and deionized water is (1-5):(5-25):(5-10); (4) The micelles are placed into a 5 mL centrifuge tube having a cover with a plurality of air holes with a diameter of 0.1-0.6 mm, thereby obtaining the attractant core I.

3. A method for preparing an attractant core II using the thrips attractant according to claim 1 as a core material, characterized in that: The specific steps include: (1) dissolving a thrips attractant in n-hexane to obtain a thrips attractant solution; (2) placing the multi-walled carbon nanotubes into a 5 mL centrifuge tube, slowly adding a thrips attractant solution, wherein the mass ratio of the thrips attractant to the multi-walled carbon nanotubes is 1:(10-20), and mixing evenly to obtain a mixture; (3) The mixture is placed on a constant temperature oscillator and oscillated at 20-25°C and 100-150 r / min for 12-24 hours to obtain the attractant core II.

4. A method for preparing an attractant core III using the thrips attractant according to claim 1 as a core material, characterized in that: Firstly, the thrips attractant is dissolved in n-hexane, and then added into a rubber carrier to obtain the attractant core III.

5. A thrips combined trapping device, characterized in that: Contains the attractant core I prepared by the preparation method as claimed in claim 2, or contains the attractant core II prepared by the preparation method as claimed in claim 3, or contains the attractant core III prepared by the preparation method as claimed in claim 4.

6. A method for preparing the thrips combined trapping device as claimed in claim 5, characterized in that: The combined trapping and killing device for thrips is obtained by hanging the trap core I prepared by the preparation method as described in claim 2 on a blue sticky insect board or a yellow sticky insect board, or hanging the trap core II prepared by the preparation method as described in claim 3 on a blue sticky insect board or a yellow sticky insect board, or hanging the trap core III prepared by the preparation method as described in claim 4 on a blue sticky insect board or a yellow sticky insect board.

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