Application of jasmonic acid inhibitor DIECA in promoting growth and development of plant seedlings

Abstract

The present invention discloses the use of a jasmonic acid inhibitor DIECA in promoting the growth and development of plant seedlings, and belongs to the technical field of plant growth promotion. The present invention uses the jasmonic acid inhibitor DIECA as a plant inducer to effectively improve the plant height, stem thickness, leaf area, and growth and development of tomato seedlings, and can increase the content of photosynthetic pigments in the leaves, as well as photosynthetic parameters such as the net photosynthetic rate, greatly improve the photosynthesis of tomato seedlings, and promote growth and development. DIECA treatment increases the stomatal conductance of tomato seedling leaves, increases the intercellular carbon dioxide content, thereby increasing the carbon fixation efficiency of the leaves, and increasing the net photosynthetic rate of the leaves; spraying DIECA promotes the accumulation of photosynthetic pigments, increases Fv / Fm, that is, increases the primary light energy conversion efficiency of photosystem II (PSII), thereby enhancing photosynthesis, and further improving the photosynthesis of the leaves.

Description

Technical Field

[0001] The present invention relates to the technical field of plant growth promotion, and in particular to use of a jasmonic acid inhibitor DIECA in promoting the growth and development of plant seedlings. Background Art

[0002] Tomato is a widely planted vegetable crop that can also be eaten as a fruit. It is rich in nutrients and has certain medical value. In recent years, it has become the main crop cultivated in facilities, and the total tomato output in my country ranks first in facility agriculture. Tomatoes have a unique flavor and high nutritional value. They are loved by the people and have a large market demand. However, at present, facility tomato cultivation in the north is often susceptible to conditions such as low temperature and weak light in winter and spring, which inhibits tomato photosynthesis and growth and development. In production, in order to ensure strong seedlings and tomato yield and quality, chemical fertilizers are often applied, but the frequent use of chemical fertilizers can easily destroy the soil structure and cause land acidification. Actively exploring a promoter that is thoroughly researched, harmless to the human body, easy to decompose, and has a significant effect when applied in trace amounts is of great significance and broad application prospects for tomato production.

[0003] Jasmonic acid is produced by a series of enzymatic reactions of substances such as linolenic acid. As a signal molecule in various tissues and organs of plants, it regulates the growth and development of plants and their adaptability to adverse environments. Jasmonic acid can also affect the absorption of nitrogen and phosphorus by plants and the transportation of organic matter such as glucose. In production, appropriate inhibition of the synthesis of jasmonic acid will promote the growth and development of plants, regulate the balance between plant growth and stress resistance, and obtain strong seedlings. Sodium diethyldithiocarbamate (DIECA) is a plant jasmonic acid (JA) synthesis pathway inhibitor. It shunts 13(S)-hydroperoxylinolenic acid in the JA pathway into 13-hydroxylinolenic acid, thereby sharply reducing the precursor pool, leading to cyclization and ultimately affecting JA synthesis. JA is a lipid-derived compound widely distributed in plants. As a growth regulator, it has an important regulatory effect on plant growth and development, such as plant root growth, tuber formation, flower development, and senescence. As one of the important signal pathways in tomato plants, JA has long been the focus of research on growth and development. However, the JA inhibitor, DIECA, has not been reported in promoting the growth and development of plant seedlings and photosynthesis. Summary of the invention

[0004] The present invention provides the use of a jasmonic acid inhibitor DIECA in promoting the growth and development of plant seedlings, which effectively improves the plant height, stem thickness, and leaf area of ​​tomato seedlings, promotes the growth and development of tomato seedlings, and can increase the content of photosynthetic pigments in leaves, as well as photosynthetic parameters such as net photosynthetic rate, greatly improves the photosynthesis of tomato seedlings, and promotes growth and development.

[0005] The invention provides use of a jasmonic acid inhibitor DIECA in promoting the growth and development of plant seedlings.

[0006] Preferably, the jasmonic acid inhibitor DIECA is used to promote photosynthesis in plant leaves.

[0007] Preferably, the method comprises the following steps: spraying an aqueous solution of the jasmonic acid inhibitor DIECA onto the stems or leaves of plant seedlings.

[0008] Preferably, the plant comprises tomato.

[0009] Preferably, the plant seedlings are at least at the two-leaf and one-heart stage.

[0010] Preferably, the preparation method of the jasmonic acid inhibitor DIECA aqueous solution is: adding distilled water to the jasmonic acid inhibitor to form a self-prepared mother solution, and adding distilled water to the self-prepared mother solution to make up to volume.

[0011] Preferably, the concentration of the self-prepared mother solution is 0.05-0.2 mol / L.

[0012] Preferably, the concentration of the jasmonic acid inhibitor DIECA aqueous solution is 100-200 μmol / L.

[0013] Preferably, the spraying amount is 30 to 60 L / mu.

[0014] Preferably, the spraying time is 8 to 10 am, and the spraying is continued for 5 to 7 days.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention uses the jasmonic acid inhibitor DIECA as a plant inducer for the first time, effectively improves the plant height, stem thickness, leaf area of ​​tomato seedlings, promotes the growth and development of tomato seedlings, and can increase the content of photosynthetic pigments in the leaves, as well as photosynthetic parameters such as net photosynthetic rate, greatly improves the photosynthesis of tomato seedlings, and promotes growth and development. After DIECA treatment, on the one hand, the stomatal conductance of the leaves of tomato seedlings becomes larger, the intercellular carbon dioxide content increases, thereby improving the carbon fixation efficiency of the leaves, and improving the net photosynthetic rate of the leaves; on the other hand, spraying DIECA promotes the accumulation of photosynthetic pigments, so that Fv / Fm increases, that is, the primary light energy conversion efficiency of photosystem II (PSII) increases, thereby enhancing photosynthesis, thereby further improving the photosynthesis of the leaves. The present invention provides an important theoretical basis for the research of tomato inducers, and also provides guidance for actual production, with broad market prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 1 is a comparison diagram of tomato seedling plant heights of Example 1 of the present invention and Comparative Example 1, wherein A is the tomato of Comparative Example 1; B is the tomato of Example 1. DETAILED DESCRIPTION

[0018] In order to enable those skilled in the art to better understand the technical solution of the present invention and implement it, the present invention is further described below in conjunction with specific examples, but the examples are not intended to limit the present invention. The following test methods and detection methods, unless otherwise specified, are conventional methods; the reagents and raw materials, unless otherwise specified, are commercially available.

[0019] The present invention uses tomato seedlings as test plants, and the cultivation of tomato seedlings includes the following steps:

[0020] S1, germination, first heat shock the tomato seeds in 55°C water for 5 min, and then culture them in a constant temperature incubator at 28°C without light until the seeds germinate;

[0021] S2, seedling raising, when the germination rate of seeds in the artificial climate box exceeds 80%, the germinated seeds can be transferred to the plug tray for seedling raising;

[0022] S3, transplanting, wait until the seedlings grow out in the plug tray, and then plant them until the seedlings grow two leaves and one heart.

[0023] Example 1

[0024] S1, preparing an aqueous solution of jasmonic acid inhibitor DIECA at a concentration of 100 μmol / L;

[0025] S2, spray the stems and leaves of tomato seedlings with the DIECA aqueous solution of S1 for 5 consecutive days, with a specific daily spraying amount of 60L / mu and a spraying time of 8-10 am.

[0026] Example 2

[0027] S1, preparing an aqueous solution of jasmonic acid inhibitor DIECA at a concentration of 100 μmol / L;

[0028] S2, spray the stems and leaves of tomato seedlings with the DIECA aqueous solution of S1 for 6 consecutive days, with a specific daily spraying amount of 50L / mu and a spraying time of 8-10 am.

[0029] Example 3

[0030] S1, preparing an aqueous solution of jasmonic acid inhibitor DIECA at a concentration of 150 μmol / L;

[0031] S2, spray the stems and leaves of tomato seedlings with the DIECA aqueous solution of S1 for 5 consecutive days, with a specific daily spraying amount of 40L / mu and a spraying time of 8-10 am.

[0032] Example 4

[0033] S1, preparing an aqueous solution of jasmonic acid inhibitor DIECA at a concentration of 200 μmol / L;

[0034] S2, spray the stems and leaves of tomato seedlings with the DIECA aqueous solution of S1 for 7 consecutive days, with a specific daily spraying amount of 30L / mu and a spraying time of 8-10 am.

[0035] In order to further illustrate the effect of the present invention, the present invention also sets a comparative example, as follows:

[0036] Comparative Example 1

[0037] Compared with Example 3, the difference is that the aqueous solution of DIECA, a jasmonic acid inhibitor, is replaced with distilled water.

[0038] Comparative Example 2

[0039] S1, preparing an aqueous solution of jasmonic acid inhibitor DIECA at a concentration of 50 μmol / L;

[0040] S2, spray the stems and leaves of tomato seedlings with the DIECA aqueous solution of S1 for 5 consecutive days, with a specific daily spraying amount of 40L / mu and a spraying time of 8-10 am.

[0041] The growth and photosynthetic parameters of the tomato seedlings treated in Examples 2 to 4 and Comparative Examples 1 to 2 were tested, and the results are shown in Tables 1, 2 and 3.

[0042] Table 1 Growth and development of tomato seedlings

[0043] Plant height / cm Stem diameter / cm <![CDATA[Leaf area / cm 2 > Fresh weight / g Dry weight / g Comparative Example 1 28.65±0.33b 0.464±0.01b 33.80±0.24b 15.77±0.25b 1.62±0.03b Comparative Example 2 27.98±0.23b 0.466±0.02b 33.56±0.15b 15.08±0.29b 1.60±0.01b Example 2 30.90±0.39a 0.49±0.01a 36.76±0.69a 16.99±0.16a 1.89±0.02a Example 3 31.10±0.32a 0.50±0.01a 36.80±0.50a 17.00±0.15a 1.90±0.02a Example 4 30.92±0.34a 0.51±0.01a 36.85±0.79a 17.12±0.19a 1.91±0.01a

[0044] Table 2 Photosynthetic pigments in tomato leaves

[0045]

[0046]

[0047] Table 3 Tomato leaf photosynthetic parameters

[0048]

[0049] As shown in Tables 1 to 3, under the action of DIECA, the plant height, stem diameter, leaf area, fresh weight and dry weight of tomato seedlings were significantly improved, which promoted the growth and development of seedlings. Figure 1As shown in the figure, the content of photosynthetic pigments and photosynthetic parameters in tomato leaves increased significantly. After DIECA treatment, on the one hand, the stomatal conductance of tomato seedling leaves increased, the intercellular carbon dioxide content increased, and the carbon fixation efficiency of the leaves increased, thereby increasing the net photosynthetic rate of the leaves; on the other hand, spraying DIECA promoted the accumulation of photosynthetic pigments, thereby increasing the photosynthesis of the leaves. And it increased Fv / Fm, that is, the primary light energy conversion efficiency of photosystem II (PSII) increased, thereby enhancing photosynthesis. Therefore, DIECA has a promoting effect on the growth and development and photosynthesis of tomato seedlings.

[0050] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. Use of the jasmonic acid inhibitor DIECA in promoting the growth and development of tomato seedlings, characterized in that: The application is to spray a DIECA aqueous solution with a concentration of 100-200 μmol / L on the stems or leaves of tomato seedlings at the two-leaf one-heart stage, with a spraying amount of 30-60 L / mu, a spraying time of 8-10 a.m., and continuous spraying for 5-7 days; the tomato growth and development promotion is: to increase the plant height, stem thickness, leaf area, fresh weight and dry weight of the tomato seedlings; to increase the chlorophyll and carotenoid content of tomato leaves, the chlorophyll includes chlorophyll a and chlorophyll b; to increase the net photosynthetic rate, transpiration rate, intercellular CO2 concentration and stomatal conductance of tomato leaves.

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