Methods to inhibit the production of zearalenone by Fusarium graminearum in agricultural products

Abstract

This application discloses a method for inhibiting the production of zearalenone by Fusarium graminearum in agricultural products. The method involves obtaining a sample to be treated, wherein the sample is an agricultural product containing Fusarium graminearum. The sample is then mixed with a photosensitizer solution to obtain a mixed sample. This mixed sample is then incubated in the dark to obtain an incubated sample. Finally, the incubated sample is irradiated with a blue light source to obtain the target sample. According to the technical solution of this application, a photodynamic method combining a photosensitizer solution and a blue light source is used to inhibit the production of zearalenone by Fusarium graminearum in agricultural products. Compared with biological methods mainly using unstable biological enzymes in related technologies, this method effectively improves the inhibition of zearalenone production by Fusarium graminearum in agricultural products.

Description

Technical Field

[0001] This application relates to, but is not limited to, the field of mycotoxin control technology, and in particular to a method for inhibiting the production of zearalenone by Fusarium graminearum in agricultural products. Background Technology

[0002] *Fusarium graminearum* is the dominant species in the *Fusarium* genus that produces mycotoxins, and zearalenone is a mycotoxin produced by *Fusarium* species, and it is one of the most common mycotoxins found in food ingredients and their byproducts. Zearalenone produced by *Fusarium graminearum* causes significant contamination of grains such as corn, wheat, rice, barley, millet, and oats. Furthermore, zearalenone has estrogenic effects, which can induce hyperestrogenosis in poultry and livestock, causing related reproductive dysfunction and even death, seriously affecting food quality and safety. Related technologies typically employ biological methods, primarily using enzyme preparations, to inhibit zearalenone in *Fusarium graminearum*, but the unstable activity of these enzymes leads to unsatisfactory toxin inhibition effects. Summary of the Invention

[0003] This application provides a method for inhibiting the production of zearalenone by Fusarium graminearum in agricultural products, which can effectively inhibit the production of zearalenone by Fusarium graminearum in agricultural products.

[0004] In a first aspect, embodiments of this application provide a method for inhibiting the production of zearalenone by Fusarium graminearum in agricultural products, comprising:

[0005] Obtain a sample to be processed, wherein the sample to be processed is an agricultural product containing Fusarium graminearum;

[0006] The sample to be treated is mixed with a photosensitizer solution to obtain a mixed sample;

[0007] The mixed sample was incubated in the dark to obtain an incubated sample;

[0008] The incubated sample was irradiated with a blue light source to obtain the target sample.

[0009] The method for inhibiting the production of zearalenone by Fusarium graminearum in agricultural products according to the first aspect of this application has at least the following beneficial effects: A sample to be treated is obtained, wherein the sample to be treated is an agricultural product containing Fusarium graminearum; the sample to be treated is then mixed with a photosensitizer solution to obtain a mixed sample; the mixed sample is then incubated in the dark to obtain an incubated sample; and the incubated sample is then irradiated with a blue light source to obtain the target sample. According to the technical solution of this application, the photodynamic method combining a photosensitizer solution and a blue light source is used to inhibit the production of zearalenone by Fusarium graminearum in agricultural products. Compared with biological methods mainly using unstable biological enzymes in related technologies, this method can effectively improve the effect of inhibiting the production of zearalenone by Fusarium graminearum in agricultural products.

[0010] According to some embodiments of the first aspect of this application, the sample to be processed is obtained by the following method:

[0011] Obtain initial Fusarium graminearum inoculum;

[0012] Based on the target absorbance, the initial Fusarium graminearum bacterial solution was diluted with a dilution solution to obtain the target bacterial solution;

[0013] The target bacterial solution is mixed with the agricultural product to obtain the sample to be treated.

[0014] According to some embodiments of the first aspect of this application, the initial Fusarium graminearum inoculum solution is obtained by the following method:

[0015] Obtain Fusarium graminearum;

[0016] The Fusarium graminearum was inoculated into a microbial culture medium to obtain the culture solution;

[0017] The bacterial culture solution is statically cultured according to the preset bacterial culture time and preset first temperature value to obtain the initial Fusarium graminearum bacterial culture solution.

[0018] According to some embodiments of the first aspect of this application, the photosensitizer solution is obtained by the following method:

[0019] Obtain the photosensitizer to be treated;

[0020] An anhydrous ethanol was used to mix the photosensitizer to be treated to obtain an initial photosensitizer solution;

[0021] The temperature of the initial photosensitizer solution is adjusted based on a preset second temperature.

[0022] The temperature of the initial photosensitizer solution is adjusted to meet a preset second temperature value to obtain the photosensitizer solution.

[0023] According to some embodiments of the first aspect of this application, the step of irradiating the incubated sample with a blue light source to obtain the target sample includes:

[0024] The incubated sample was placed inside a shielded light box;

[0025] The incubation sample is irradiated with a blue light source inside the shielded light box to obtain the target sample.

[0026] According to some embodiments of the first aspect of this application, the photosensitizer to be treated is curcumin.

[0027] According to some embodiments of the first aspect of this application, the irradiation time of the incubated sample using a blue light source is 30 min to 120 min.

[0028] According to some embodiments of the first aspect of this application, the incubation time for incubating the mixed sample in the dark is 20 min to 50 min.

[0029] According to some embodiments of the first aspect of this application, the photosensitizer concentration in the mixed sample is from 50 μmol / L to 200 μmol / L.

[0030] According to some embodiments of the first aspect of this application, the wavelength of the blue light source is 440 nm to 460 nm. Attached Figure Description

[0031] The accompanying drawings are provided to further understand the technical solutions of the present invention and constitute a part of the specification. They are used together with the embodiments of the present invention to explain the technical solutions of the present invention, and do not constitute a limitation on the technical solutions of the present invention.

[0032] Figure 1 This is a flowchart of the steps of a method for inhibiting the production of zearalenone by Fusarium graminearum in agricultural products according to an embodiment of this application;

[0033] Figure 2 This is a flowchart of the steps of a method for obtaining a sample to be processed according to another embodiment of this application;

[0034] Figure 3 This is a flowchart of the steps for obtaining initial Fusarium graminearum inoculum according to another embodiment of this application;

[0035] Figure 4 This is a flowchart of a method for obtaining a photosensitizer solution according to another embodiment of this application;

[0036] Figure 5 This is a flowchart of the steps for obtaining a target sample according to another embodiment of this application;

[0037] Figure 6 This is a comparison diagram of the effect of different irradiation durations on the colony diameter of Fusarium graminearum provided in another embodiment of this application;

[0038] Figure 7 This is a line graph showing the effect of different irradiation durations on the colony diameter of Fusarium graminearum, provided in another embodiment of this application.

[0039] Figure 8 This is a comparison diagram of the effect of different photosensitizer concentrations on the colony diameter of Fusarium graminearum provided in another embodiment of this application;

[0040] Figure 9 This is a line graph showing the effect of different photosensitizer concentrations on the colony diameter of Fusarium graminearum, provided in another embodiment of this application.

[0041] Figure 10 This is a bar chart showing the content of zearalenone in zearalenone under different treatment conditions provided in another embodiment of this application. Detailed Implementation

[0042] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application.

[0043] It is understandable that although functional modules are divided in the device schematic diagram and a logical order is shown in the flowchart, in some cases, the steps shown or described may be performed in a different order than the module division in the device or the order in the flowchart. The terms "first," "second," etc., in the specification, claims, or the aforementioned drawings are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence.

[0044] This application provides a method for inhibiting the production of zearalenone by Fusarium graminearum in agricultural products. The method involves obtaining a sample to be treated, wherein the sample is an agricultural product infected with Fusarium graminearum. The sample is then mixed with a photosensitizer solution to obtain a mixed sample. This mixed sample is then incubated in the dark to obtain an incubated sample. Finally, the incubated sample is irradiated with a blue light source to obtain the target sample. According to the technical solution of this application, a photodynamic method combining a photosensitizer solution and a blue light source is used to inhibit the production of zearalenone by Fusarium graminearum in agricultural products. Compared with biological methods mainly using unstable biological enzymes in related technologies, this method effectively improves the inhibition of zearalenone production by Fusarium graminearum in agricultural products.

[0045] The embodiments of this application will be further described below with reference to the accompanying drawings.

[0046] Reference Figure 1 , Figure 1 This is a flowchart illustrating the steps of a method for inhibiting the production of zearalenone by Fusarium graminearum in agricultural products, according to an embodiment of this application. The method includes, but is not limited to, the following steps:

[0047] Step S110: Obtain the sample to be processed, which is an agricultural product containing Fusarium graminearum;

[0048] Step S120: Mix the sample to be treated with the photosensitizer solution to obtain a mixed sample;

[0049] Step S130: Incubate the mixed sample in the dark to obtain an incubated sample;

[0050] Step S140: Irradiate the incubated sample with a blue light source to obtain the target sample.

[0051] It should be noted that this application does not limit the specific method of obtaining the sample to be processed. It can be prepared using Fusarium graminearum and agricultural products, or it can be obtained directly from readily available agricultural products containing Fusarium graminearum. The embodiments of this application also do not limit the specific type of photosensitizer solution; it can be a curcumin solution or a curcumin-bridged porphyrin photosensitizer solution.

[0052] It is understandable that irradiating the incubated sample with a blue light source excites the photosensitizer within the sample. The excited photosensitizer then transfers energy to the surrounding oxygen, generating singlet oxygen. Since singlet oxygen is a highly reactive oxygen species with strong oxidizing properties, it can damage the macromolecular structure of cells, leading to cell necrosis and apoptosis. Therefore, irradiating the incubated sample with a blue light source can inhibit the growth of *Fusarium graminearum* and the production of zearalenone by *Fusarium graminearum*.

[0053] Understandably, agricultural products containing *Fusarium graminearum* are obtained as samples to be treated. These samples are then mixed with a photosensitizer solution to obtain a mixed sample, which enhances the photosensitivity of the mixed sample. The mixed sample is then incubated in the dark to obtain an incubated sample, allowing for better binding of *Fusarium graminearum* in the mixed sample with the photosensitizer in the photosensitizer solution. Finally, the incubated sample is irradiated with a blue light source to inhibit the growth of *Fusarium graminearum* and the production of zearalenone by *Fusarium graminearum*, resulting in a highly safe target sample. According to the technical solution of this application, the photodynamic method combining a photosensitizer solution and a blue light source effectively inhibits the production of zearalenone by *Fusarium graminearum* in agricultural products. Compared to biological methods in related technologies that primarily use unstable biological enzymes, this method significantly improves the inhibition of zearalenone production by *Fusarium graminearum* in agricultural products.

[0054] Additionally, refer to Figure 2In one embodiment, Figure 1 The sample to be processed in step S110 of the illustrated embodiment can be obtained through the following method steps:

[0055] Step S210: Obtain the initial Fusarium graminearum inoculum solution;

[0056] Step S220: Based on the target absorbance, the initial Fusarium graminearum culture is diluted with a dilution solution to obtain the target culture.

[0057] Step S230: Mix the target bacterial solution with agricultural products to obtain the sample to be treated.

[0058] It should be noted that the embodiments of this application do not limit the specific value of the target absorbance; it can be 0.6 or 0.8. It is understood that when the absorbance of the initial *Fusarium graminearum* bacterial solution is 0.6, it indicates that the *Fusarium graminearum* in the initial bacterial solution is in the logarithmic growth phase, that is, the *Fusarium graminearum* is in a state of vigorous growth.

[0059] In addition, it should be noted that the embodiments of this application do not limit the specific type of dilution solution; it can be 0.9% physiological saline or distilled water.

[0060] Understandably, the initial Fusarium graminearum bacterial solution is obtained, and based on the target absorbance, it is diluted with a dilution solution to obtain the target bacterial solution. This ensures that the Fusarium graminearum in the target bacterial solution is in a vigorous growth state, so as to facilitate subsequent observation of the effect of inhibiting the production of zearalenone by Fusarium graminearum. The target bacterial solution is then mixed with agricultural products to obtain the sample to be treated.

[0061] Additionally, refer to Figure 3 In one embodiment, Figure 2 The initial Fusarium graminearum culture in step S210 of the illustrated embodiment can be obtained through the following method steps:

[0062] Step S310: Obtain Fusarium graminearum;

[0063] Step S320: Inoculate Fusarium graminearum into a microbial culture medium to obtain the culture solution;

[0064] Step S330: According to the preset bacterial culture time and preset first temperature value, the bacterial culture to be cultured is allowed to stand still to obtain the initial Fusarium graminearum bacterial culture.

[0065] It should be noted that this application does not limit the specific type of microbial culture medium. It can be potato dextrose broth (PDB), or a microbial culture medium composed of 20% potato dextrose agar, 1L potato juice, 20g glucose, 3g potassium dihydrogen phosphate, 4.7g magnesium sulfate, 1.5g water, trace amounts of thiamine, and 15g agar. This application also does not limit the specific values ​​of the preset bacterial culture time and the preset first temperature. The preset bacterial culture time can be 24h to 48h, and the preset first temperature can be 37℃.

[0066] It is understandable that Fusarium graminearum can be Fusarium graminearum stored at -80℃. Fusarium graminearum stored at -80℃ has a higher survival rate of mycelium and conidia, and better culture characteristics and sporulation ability.

[0067] It is understandable that inoculating Fusarium graminearum into a microbial culture medium provides the nutrients required for its maintenance and growth, resulting in a culture solution. Then, according to a preset culture time and a preset first temperature value, the culture solution is allowed to stand for a period of time to obtain an initial Fusarium graminearum culture solution, which can improve the stability of Fusarium graminearum growth.

[0068] Additionally, refer to Figure 4 In one embodiment, Figure 1 The photosensitizer solution in step S120 of the illustrated embodiment can be obtained by the following method steps:

[0069] Step S410: Obtain the photosensitizer to be treated;

[0070] Step S420: Mix anhydrous ethanol with the photosensitizer to be treated to obtain an initial photosensitizer solution;

[0071] Step S430: Based on the preset second temperature, adjust the temperature of the initial photosensitizer solution to meet the preset second temperature value, and obtain the photosensitizer solution.

[0072] It should be noted that this application does not limit the specific type of photosensitizer to be treated; it can be curcumin or curcumin-bridged porphyrin photosensitizer. This application also does not limit the specific value of the preset second temperature; it can be -4℃. It is understood that a photosensitizer solution at -4℃ is easier to preserve and ensures the performance of the photosensitizer solution.

[0073] It is understandable that the obtained photosensitizer solution should be stored away from light to prevent the photosensitizer solution from undergoing chemical and / or physical reactions when exposed to light, thus ensuring the performance of the photosensitizer solution.

[0074] It is understandable that mixing anhydrous ethanol with the photosensitizer to be treated allows the photosensitizer to be fully dissolved in the anhydrous ethanol, resulting in an initial photosensitizer solution. Subsequently, based on a preset second temperature, the temperature of the initial photosensitizer solution is adjusted to meet the preset second temperature value, thus obtaining a photosensitizer solution. This avoids chemical and / or physical reactions in the photosensitizer solution, ensuring its performance and improving the efficiency of inhibiting the production of zearalenone by Fusarium graminearum in agricultural products.

[0075] Additionally, refer to Figure 5 In one embodiment, Figure 1 Step S140 in the illustrated embodiment also includes, but is not limited to, the following steps:

[0076] Step S510: Place the incubation sample inside a shielded light box;

[0077] Step S520: Irradiate the incubated sample with a blue light source inside a shielded light box to obtain the target sample.

[0078] It should be noted that this application does not limit the specific type of shielded light box; it can be a metal sealed light box or an electromagnetic shielded light box.

[0079] It is understandable that placing the incubation sample inside a shielded light box and irradiating it with a blue light source inside the box can prevent other light sources from irradiating the sample, thereby improving the efficiency of obtaining the target sample.

[0080] In one embodiment, the photosensitizer to be treated is curcumin.

[0081] It is understandable that curcumin, a polyphenolic compound extracted from the rhizome of the turmeric plant (Zingiberaceae family), is a natural food additive and photosensitizer. It boasts advantages such as wide availability, low cost, non-toxicity, and no pollution, as well as antibacterial and antitumor functions. Using curcumin as a photosensitizer can effectively reduce the cost of inhibiting the production of zearalenone by Fusarium graminearum in agricultural products, while avoiding contamination and ensuring the safety of agricultural products.

[0082] In one embodiment, the irradiation time of the incubated sample using a blue light source is 30 min to 120 min.

[0083] It is understandable that irradiating the incubated samples with a blue light source for 30 to 120 minutes can effectively inhibit the growth of Fusarium graminearum and the production of zearalenone by Fusarium graminearum. More preferably, the irradiation time of the incubated samples with a blue light source can be 60 to 120 minutes.

[0084] Understandably, given a photosensitizer concentration (Curcunim) of 100 μmol / L in the mixed sample and an incubation time of 30 min in the dark, the growth of *Fusarium graminearum* varies with different blue light irradiation durations. (Refer to...) Figure 6 When the samples were irradiated with blue light for 0 min, 30 min, 60 min, 90 min, and 120 min, the colony diameters of *Fusarium graminearum* in the samples were not the same. Multiple samples obtained with different irradiation times were placed in a constant temperature incubator for cultivation. At a cultivation time of 72 h, the colony diameter of *Fusarium graminearum* with an irradiation time of 30 min was 16.8 mm, with 16.1 mm for 60 min, 12.3 mm for 90 min, and only 10.5 mm for 120 min. This indicates that photosensitizer-mediated photodynamic inactivation (PDI), i.e., the photodynamic method combining photosensitizer solution and blue light source, can effectively inhibit the growth of *Fusarium graminearum*.

[0085] Additionally, refer to Figure 6 and Figure 7 With a photosensitizer concentration of 0 μmol / L in the mixed sample and an incubation time of 30 min in the dark, followed by irradiation with a blue light source for 0 min, the resulting sample was placed in a constant temperature incubator for 72 h. The diameter of the *Fusarium graminearum* colony was greater than 40 mm. Similarly, with a photosensitizer concentration of 100 μmol / L in the mixed sample and an incubation time of 30 min in the dark, followed by irradiation with a blue light source for 0 min, the resulting sample was placed in a constant temperature incubator for 72 h. The diameter of the *Fusarium graminearum* colony was greater than 40 mm. The methods described above—such as directly irradiating agricultural products infected with Fusarium graminearum using a blue light source without mixing the sample to be treated with the photosensitizer solution, and irradiating a mixed sample of the sample to be treated with the photosensitizer solution but without using a blue light source—have extremely low effects in inhibiting the growth of Fusarium graminearum. According to the technical solution of this application, the photodynamic method combining the photosensitizer solution and a blue light source can effectively inhibit the growth of Fusarium graminearum.

[0086] In one embodiment, the incubation time for the mixed sample in the dark is 20 min to 50 min.

[0087] It is understood that incubating the mixed sample in the dark for 20 to 50 minutes allows for better binding of *Fusarium graminearum* in the mixed sample with the photosensitizer in the photosensitizer solution, thereby improving the efficiency of inhibiting the production of zearalenone by *Fusarium graminearum* in agricultural products. Preferably, the incubation time for the mixed sample in the dark can be 25 to 45 minutes. More preferably, the incubation time for the mixed sample in the dark can be 30 minutes.

[0088] In one embodiment, the photosensitizer concentration in the mixed sample is from 50 μmol / L to 200 μmol / L.

[0089] It is understood that a photosensitizer concentration of 50 μmol / L to 200 μmol / L in the mixed sample can effectively inhibit the production of zearalenone by Fusarium graminearum in agricultural products. More preferably, the photosensitizer concentration in the mixed sample can be 50 μmol / L to 200 μmol / L.

[0090] Understandably, referring to Figure 8 and Figure 9 When the mixed sample was incubated in the dark for 60 min and then irradiated with a blue light source for 60 min, the colony diameter of Fusarium graminearum in the samples with photosensitizer concentrations of 0 μmol / L, 50 μmol / L, 70 μmol / L, 100 μmol / L and 150 μmol / L were not the same. Multiple samples obtained with different photosensitizer concentrations were placed in a constant temperature incubator for cultivation. After 72 hours of cultivation, the colony diameter of *Fusarium graminearum* in the mixed sample with a photosensitizer concentration of 50 μmol / L was 26.5 mm, the colony diameter of *Fusarium graminearum* in the mixed sample with a photosensitizer concentration of 70 μmol / L was 25.7 mm, and the colony diameter of *Fusarium graminearum* in the mixed sample with a photosensitizer concentration of 100 μmol / L was 14.2 mm, indicating a high degree of inhibition of *Fusarium graminearum* growth. The colony diameter of *Fusarium graminearum* in the mixed sample with a photosensitizer concentration of 150 μmol / L was only 3.8 mm. This demonstrates that photosensitizer-mediated photodynamic sterilization, i.e., the photodynamic method combining photosensitizer solution and blue light source, can effectively inhibit the growth of *Fusarium graminearum*.

[0091] In one embodiment, the wavelength of the blue light source is 440 nm to 460 nm.

[0092] Understandably, blue light sources with wavelengths of 440nm to 460nm are high-energy rays capable of penetrating the cell membrane and nucleus of *Fusarium graminearum*, disrupting DNA bonds and rendering the fungus inactive. This inhibits the growth of *Fusarium graminearum* and the production of zearalenone, ensuring the safety of agricultural products. Blue light sources can also have wavelengths of 430nm to 440nm or 460nm to 480nm, which will not be discussed further here.

[0093] It is understandable that the light energy density of a blue light source is 6 J / cm². 2 Up to 50J / cm 2 This ensures more precise blue light illumination and guarantees the effectiveness of the blue light source. Preferably, the light energy density of the blue light source can be 9.36 J / cm². 2 Up to 50.6 J / cm 2 More preferably, the light energy density of the blue light source can be 12.48 J / cm². 2 Up to 43.0 J / cm 2 .

[0094] Understandably, the distance between the blue light source and the incubated sample is 10cm to avoid the blue light source being too close and affecting the original flavor and color of the agricultural products.

[0095] It is understandable that the blue light source can be an LED blue light source. LED blue light sources have advantages such as low power consumption, long service life, energy saving and good applicability. Using LED blue light sources can reduce the cost of inhibiting the production of zearalenone by Fusarium graminearum in agricultural products.

[0096] In one embodiment, sterile corn was mixed with Fusarium graminearum inoculum and treated, then divided into three groups. Group 1: photosensitizer concentration of 200 μmol / L, incubation in darkness for 30 min, and irradiation with a blue light source for 0 min; Group 2: photosensitizer concentration of 0 μmol / L, incubation in darkness for 30 min, and irradiation with a blue light source for 60 min; Group 3: photosensitizer concentrations of 50 μmol / L, 100 μmol / L, 150 μmol / L, and 200 μmol / L, respectively, incubation in darkness for 30 min, and irradiation with a blue light source for 60 min. The light energy density of the blue light source was 43.0 J / cm². 2 The samples obtained from each group were drained and placed in a constant temperature incubator at 25℃ for 10 days.

[0097] The processed corn was pulverized to obtain corn flour, which was then sieved through a 60-mesh sieve. 2.00 g of the sieved corn flour was weighed and placed in a 50 mL centrifuge tube. 2.00 mL of deionized water was added, and the mixture was vortexed and allowed to stand for 1 hour. Then, 4.00 mL of acetonitrile solution containing 1% acetic acid was added, the mixture was shaken for 2 minutes, and sonicated for 30 minutes. After sonication, the mixture was centrifuged at 5000 rpm for 5 minutes, and the supernatant was filtered through a membrane for liquid chromatography analysis. (Refer to...) Figure 10 The results showed that the ZEN content in maize treated in group 1 reached 1.15 mg / kg, the ZEN content in group 2 reached 1.12 mg / kg, and in all three groups, the ZEN content gradually decreased with increasing photosensitizer concentration. When the photosensitizer concentration was greater than 100 μmol / L, ZEN toxin was not detected in any of the maize samples. Therefore, the photodynamic method combining photosensitizer solution and blue light source can effectively inhibit the production of ZEN by Fusarium graminearum.

[0098] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0099] The above is a detailed description of the preferred embodiments of this application. However, this application is not limited to the above embodiments. Those skilled in the art can make various equivalent modifications or substitutions without departing from the spirit of this application. All such equivalent modifications or substitutions are included within the scope defined by the claims of this application.

Claims

1. A method of inhibiting the production of zearalenone by Fusarium graminearum in an agricultural product, characterized by, The application relates to a method for treating Fusarium graminearum in agricultural products. The method comprises the following steps: acquiring a to-be-treated sample, the to-be-treated sample being an agricultural product with Fusarium graminearum, wherein the Fusarium graminearum is derived from a target bacterial liquid, and the absorbance of the target bacterial liquid is 0.6; mixing the to-be-treated sample with a photosensitizer solution to obtain a mixed sample, the photosensitizer solution being prepared by dissolving curcumin in anhydrous ethanol and being stored at-4 DEG C, and the concentration of the photosensitizer in the mixed sample being 50 mu mol / L to 200 mu mol / L; The incubated sample is placed in a shielded light box, and irradiated with blue light having a wavelength of 440 to 460 nm and a light energy density of 6-50 The incubated sample is irradiated with a blue light source to obtain a target sample.

2. The method of inhibiting the production of zearalenone by Fusarium graminearum in an agricultural product of claim 1, wherein, incubating the mixed sample in the dark for 20 to 50 minutes to obtain an incubated sample; the to-be-treated sample is obtained by the following method: acquiring an initial Fusarium graminearum bacterial liquid; based on a target absorbance, diluting the initial Fusarium graminearum bacterial liquid with a dilution solution to obtain a target bacterial liquid; 3. The method for inhibiting the production of zearalenone by Fusarium graminearum in an agricultural product according to claim 2, characterized by, mixing the target bacterial liquid with the agricultural product to obtain the to-be-treated sample. The initial Fusarium graminearum bacterial liquid is obtained by the following method: acquiring Fusarium graminearum; inoculating the Fusarium graminearum into a microbial culture medium to obtain a to-be-cultured bacterial liquid; 4. The method for inhibiting the production of zearalenone by Fusarium graminearum in an agricultural product according to claim 1, characterized by, according to a preset bacterial liquid culture duration and a preset first temperature value, incubating the to-be-cultured bacterial liquid to obtain the initial Fusarium graminearum bacterial liquid. The photosensitizer solution is obtained by the following method: acquiring a to-be-treated photosensitizer; mixing anhydrous ethanol with the to-be-treated photosensitizer to obtain an initial photosensitizer solution; adjusting the temperature of the initial photosensitizer solution based on a preset second temperature, 5. The method for inhibiting the production of zearalenone by Fusarium graminearum in an agricultural product according to claim 4, characterized by, adjusting the temperature value of the initial photosensitizer solution to meet the preset second temperature value to obtain the photosensitizer solution.

6. The method for inhibiting the production of zearalenone by Fusarium graminearum in an agricultural product according to claim 1, characterized by, The to-be-treated photosensitizer is curcumin. The irradiation duration of the incubated sample by the blue light source is 30 min to 120 min.

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